P30297
type: Protein
reviewed: true
created: 1993-04-01
modified: 2023-05-03
version: 82
mnemonic: 1A1D_PSES0
old mnemonic: A1AD_PSES0
citation: http://purl.uniprot.org/citations/1821764
recommended name: SIP830A7BA9C67F8261
enzyme: http://purl.uniprot.org/enzyme/3.5.99.7
organism: http://purl.uniprot.org/taxonomy/29439
annotation: SIP723D2971AFEA1B61
annotation: SIP6F7BE937D33F4BA2
annotation: SIP411E409D6AADFD1F
annotation: SIP1899C6C9068B9AAF
annotation: SIP198D276109EA4D6C
annotation: SIP71670DAD9B50DEF0
annotation: PRO_0000184503
annotation: SIPE20A4FFF47E0053A
annotation: SIPA13B80DC94896A1F
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/308
classified with: http://purl.uniprot.org/keywords/378
classified with: http://purl.uniprot.org/keywords/663
classified with: GO_0008660
classified with: GO_0030170
classified with: GO_0018871
classified with: GO_0009310
sequence: P30297-1

P30297-citation-1821764
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

cd06449
type: Resource
database: CDD
comment: ACCD
signature sequence match: CDD cd06449 match 1

MF_00807
type: Resource
database: HAMAP
comment: ACC_deaminase
signature sequence match: HAMAP MF 00807 match 1

IPR027278
type: Resource
database: InterPro
comment: ACCD_DCysDesulf

IPR005965
type: Resource
database: InterPro
comment: ACP_carboxylate_deaminase

IPR020601
type: Resource
database: InterPro
comment: ACP_carboxylate_deaminase_bac

IPR001926
type: Resource
database: InterPro
comment: TrpB-like_PALP

IPR036052
type: Resource
database: InterPro
comment: TrpB-like_PALP_sf

PTHR43780
type: Resource
database: PANTHER
comment: 1-AMINOCYCLOPROPANE-1-CARBOXYLATE DEAMINASE-RELATED
signature sequence match: PANTHER PTHR43780 match 1

PTHR43780:SF2
type: Resource
database: PANTHER
comment: 1-AMINOCYCLOPROPANE-1-CARBOXYLATE DEAMINASE-RELATED
signature sequence match: PANTHER PTHR43780:SF2 match 1

PF00291
type: Resource
database: Pfam
comment: PALP
signature sequence match: Pfam PF00291 match 1

PIRSF006278
type: Resource
database: PIRSF
comment: ACCD_DCysDesulf
signature sequence match: PIRSF PIRSF006278 match 1

SSF53686
type: Resource
database: SUPFAM
comment: Tryptophan synthase beta subunit-like PLP-dependent enzymes
signature sequence match: SUPFAM SSF53686 match 1

TIGR01274
type: Resource
database: TIGRFAMs
comment: ACC_deam
signature sequence match: TIGRFAMs TIGR01274 match 1

SIP830A7BA9C67F8261
type: Structured Name
full name: 1-aminocyclopropane-1-carboxylate deaminase
short name: ACC deaminase
short name: ACCD
ec name: 3.5.99.7

acdS
type: Gene

SIP723D2971AFEA1B61
type: Function Annotation
comment: Catalyzes a cyclopropane ring-opening reaction, the irreversible conversion of 1-aminocyclopropane-1-carboxylate (ACC) to ammonia and alpha-ketobutyrate. Allows growth on ACC as a nitrogen source.

SIP1899C6C9068B9AAF
type: Subunit Annotation
comment: Homotrimer.

SIP198D276109EA4D6C
type: Biotechnology Annotation
comment: Introduced by genetic manipulation and expressed in improved ripening tomato by Monsanto. ACC is the immediate precursor of the phytohormone ethylene which is involved in the control of ripening. ACC deaminase reduces ethylene biosynthesis and thus extends the shelf life of fruits and vegetables.

SIP71670DAD9B50DEF0
type: Similarity Annotation
comment: Belongs to the ACC deaminase/D-cysteine desulfhydrase family.

PRO_0000184503
type: Chain Annotation
comment: 1-aminocyclopropane-1-carboxylate deaminase
mass: 36873
range: 22574280230909742tt1tt338

22574280230909742tt1
type: Position
type: ExactPosition
position: 1
reference: P30297-1

22574280230909742tt338
type: Position
type: ExactPosition
position: 338
reference: P30297-1

SIPE20A4FFF47E0053A
type: Active Site Annotation
comment: Nucleophile
range: 22574280230909742tt78tt78

22574280230909742tt78
type: Position
type: ExactPosition
position: 78
reference: P30297-1

SIPA13B80DC94896A1F
type: Modified Residue Annotation
comment: N6-(pyridoxal phosphate)lysine
range: 22574280230909742tt51tt51

22574280230909742tt51
type: Position
type: ExactPosition
position: 51
reference: P30297-1

P30297-1
type: Simple Sequence
modified: 1993-04-01
version: 1
mass: 36873
value: MNLNRFERYPLTFGPSPITPLKRLSQHLGGKVELYAKREDCNSGLAFGGNKTRKLEYLIPEAIEQGCDTLVSIGGIQSNQTRQVAAVAAHLGMKCVLVQENWVNYSDAVYDRVGNIEMSRIMGADVRLDAAGFDIGIRPSWEKAMSDVVEQGGKPFPIPAGCSEHPYGGLGFVGFAEEVRQQEKELGFKFDYIVVCSVTGSTQAGMVVGFAADGRSKNVIGIDASAKPEQTKAQILRIARHTAELVELGREITEEDVVLDTRFAYPEYGLPNEGTLEAIRLCGSLEGVLTDPVYEGKSMHGMIEMVRRGEFPEGSKVLYAHLGGAPALNAYSFLFRNG

http://purl.uniprot.org/uniprot/P30297#attribution-EF49CFF7CB7DF66E7BB7BA4F7CE54F55
manual: true
evidence: ECO_0000255
source: MF_00807

Q00740
type: Protein
reviewed: true
created: 1993-04-01
modified: 2023-05-03
version: 94
mnemonic: 1A1D_PSEUD
old mnemonic: 1A1D_PSESP
citation: http://purl.uniprot.org/citations/1885510
recommended name: SIP830A7BA9C67F8261
enzyme: http://purl.uniprot.org/enzyme/3.5.99.7
organism: http://purl.uniprot.org/taxonomy/74568
annotation: SIPBC4765B974B3F2F4
annotation: SIPF1D064A8127660CA
annotation: SIP535B1DD0544D8C4F
annotation: SIP19A70272D44DBAD6
annotation: SIP18D16278380CA15B
annotation: PRO_0000184505
annotation: SIPF14BCB8942D1449A
annotation: SIP72E5392805704D97
annotation: SIPA3C40E22444300C1
annotation: SIPAE13F843055E3593
annotation: SIP0F15FC1368CCA19D
annotation: SIP54F53033774AF79D
annotation: SIP575BB5736106082A
annotation: SIP158F6D83DFC3D049
annotation: SIPA40B0C07C6744978
annotation: SIPCC4505CEAEB0B68D
annotation: SIPB740BCC687071F4E
annotation: SIPEFC3AC47E696F66C
annotation: SIP8896563A59D102E9
annotation: SIPAB2B90FA08CF5D3D
annotation: SIP541651CB96CD25A4
annotation: SIP3BF5FAFED4E12B3A
annotation: SIP749906341274A1DF
annotation: SIP68286B4EE272286A
annotation: SIP19DF85B8F8340BE6
annotation: SIP3E3CC4950BEE037B
annotation: SIP83B3F3ABDF2C0B87
annotation: SIP22D289E7DDDE169A
annotation: SIP242B6161EFDD2914
annotation: SIP52B3693305CFC9BE
annotation: SIPF88FCEE571077859
annotation: SIP48D8529E015DEC6C
annotation: SIP1F30FF5E34A4983D
annotation: SIP41380600F60D47AF
annotation: SIP603EA80AA8D3DC26
annotation: SIP22087BD08A5D55F8
annotation: SIPFE8DE9F3E4EF60CE
annotation: SIPDD27A3C1762D01DB
annotation: SIPF03510A9B0277E74
annotation: SIPD3F84C96BE4F86D1
annotation: SIPB98C8230E27E4BA6
annotation: SIPC8CA2C5970E161E1
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/2
classified with: http://purl.uniprot.org/keywords/903
classified with: http://purl.uniprot.org/keywords/378
classified with: http://purl.uniprot.org/keywords/663
classified with: GO_0008660
classified with: GO_0030170
classified with: GO_0018871
classified with: GO_0009310
sequence: Q00740-1

Q00740-citation-1885510
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
scope: PROTEIN SEQUENCE
scope: FUNCTION
scope: CATALYTIC ACTIVITY
scope: COFACTOR

1RQX
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.50
chain sequence mapping: PDB 1RQX tt1tt338

PDB 1RQX tt1tt338
chain: A/B/C/D=1-338

1TYZ
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.00
chain sequence mapping: PDB 1TYZ tt1tt338

PDB 1TYZ tt1tt338
chain: A/B/C/D=1-338

1TZ2
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.10
chain sequence mapping: PDB 1TZ2 tt1tt338

PDB 1TZ2 tt1tt338
chain: A/B/C/D=1-338

1TZJ
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 1.99
chain sequence mapping: PDB 1TZJ tt1tt338

PDB 1TZJ tt1tt338
chain: A/B/C/D=1-338

1TZK
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.00
chain sequence mapping: PDB 1TZK tt1tt338

PDB 1TZK tt1tt338
chain: A/B/C/D=1-338

1TZM
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.08
chain sequence mapping: PDB 1TZM tt1tt338

PDB 1TZM tt1tt338
chain: A/B/C/D=1-338

DB02085
type: Resource
database: DrugBank
comment: 1-Aminocyclopropanecarboxylic Acid

DB03053
type: Resource
database: DrugBank
comment: 1-Aminocyclopropylphosphonate

DB01804
type: Resource
database: DrugBank
comment: 2-Ammoniobut-3-Enoate, 2-Amino-3-Butenoate

DB04212
type: Resource
database: DrugBank
comment: 2-Iminiopropanoate

DB04553
type: Resource
database: DrugBank
comment: 2-Oxobutanoic Acid

DB01735
type: Resource
database: DrugBank
comment: 3-Chloroalaninate

http://purl.uniprot.org/brenda/3.5.99.7
type: Resource
database: BRENDA
comment: 5085

SIP830A7BA9C67F8261
type: Structured Name
full name: 1-aminocyclopropane-1-carboxylate deaminase
short name: ACC deaminase
short name: ACCD
ec name: 3.5.99.7

acdS
type: Gene

SIPBC4765B974B3F2F4
type: Function Annotation
comment: Catalyzes a cyclopropane ring-opening reaction, the irreversible conversion of 1-aminocyclopropane-1-carboxylate (ACC) to ammonia and alpha-ketobutyrate. Allows growth on ACC as a nitrogen source.

SIP19A70272D44DBAD6
type: Subunit Annotation
comment: Homotrimer.

SIP18D16278380CA15B
type: Similarity Annotation
comment: Belongs to the ACC deaminase/D-cysteine desulfhydrase family.

PRO_0000184505
type: Chain Annotation
comment: 1-aminocyclopropane-1-carboxylate deaminase
mass: 36672
range: 22852456756293678tt1tt338

22852456756293678tt1
type: Position
type: ExactPosition
position: 1
reference: Q00740-1

22852456756293678tt338
type: Position
type: ExactPosition
position: 338
reference: Q00740-1

SIPF14BCB8942D1449A
type: Active Site Annotation
comment: Nucleophile
range: 22852456756293678tt78tt78

22852456756293678tt78
type: Position
type: ExactPosition
position: 78
reference: Q00740-1

SIP72E5392805704D97
type: Modified Residue Annotation
comment: N6-(pyridoxal phosphate)lysine
range: 22852456756293678tt51tt51

22852456756293678tt51
type: Position
type: ExactPosition
position: 51
reference: Q00740-1

22852456756293678tt3
type: Position
type: ExactPosition
position: 3
reference: Q00740-1

22852456756293678tt5
type: Position
type: ExactPosition
position: 5
reference: Q00740-1

22852456756293678tt12
type: Position
type: ExactPosition
position: 12
reference: Q00740-1

22852456756293678tt15
type: Position
type: ExactPosition
position: 15
reference: Q00740-1

22852456756293678tt18
type: Position
type: ExactPosition
position: 18
reference: Q00740-1

22852456756293678tt20
type: Position
type: ExactPosition
position: 20
reference: Q00740-1

22852456756293678tt22
type: Position
type: ExactPosition
position: 22
reference: Q00740-1

22852456756293678tt27
type: Position
type: ExactPosition
position: 27
reference: Q00740-1

22852456756293678tt30
type: Position
type: ExactPosition
position: 30
reference: Q00740-1

22852456756293678tt38
type: Position
type: ExactPosition
position: 38
reference: Q00740-1

22852456756293678tt39
type: Position
type: ExactPosition
position: 39
reference: Q00740-1

22852456756293678tt41
type: Position
type: ExactPosition
position: 41
reference: Q00740-1

22852456756293678tt50
type: Position
type: ExactPosition
position: 50
reference: Q00740-1

22852456756293678tt56
type: Position
type: ExactPosition
position: 56
reference: Q00740-1

22852456756293678tt59
type: Position
type: ExactPosition
position: 59
reference: Q00740-1

22852456756293678tt64
type: Position
type: ExactPosition
position: 64
reference: Q00740-1

22852456756293678tt69
type: Position
type: ExactPosition
position: 69
reference: Q00740-1

22852456756293678tt75
type: Position
type: ExactPosition
position: 75
reference: Q00740-1

22852456756293678tt79
type: Position
type: ExactPosition
position: 79
reference: Q00740-1

22852456756293678tt91
type: Position
type: ExactPosition
position: 91
reference: Q00740-1

22852456756293678tt94
type: Position
type: ExactPosition
position: 94
reference: Q00740-1

22852456756293678tt100
type: Position
type: ExactPosition
position: 100
reference: Q00740-1

22852456756293678tt108
type: Position
type: ExactPosition
position: 108
reference: Q00740-1

22852456756293678tt112
type: Position
type: ExactPosition
position: 112
reference: Q00740-1

22852456756293678tt114
type: Position
type: ExactPosition
position: 114
reference: Q00740-1

22852456756293678tt121
type: Position
type: ExactPosition
position: 121
reference: Q00740-1

22852456756293678tt125
type: Position
type: ExactPosition
position: 125
reference: Q00740-1

22852456756293678tt128
type: Position
type: ExactPosition
position: 128
reference: Q00740-1

22852456756293678tt135
type: Position
type: ExactPosition
position: 135
reference: Q00740-1

22852456756293678tt138
type: Position
type: ExactPosition
position: 138
reference: Q00740-1

22852456756293678tt140
type: Position
type: ExactPosition
position: 140
reference: Q00740-1

22852456756293678tt150
type: Position
type: ExactPosition
position: 150
reference: Q00740-1

22852456756293678tt155
type: Position
type: ExactPosition
position: 155
reference: Q00740-1

22852456756293678tt157
type: Position
type: ExactPosition
position: 157
reference: Q00740-1

22852456756293678tt160
type: Position
type: ExactPosition
position: 160
reference: Q00740-1

22852456756293678tt162
type: Position
type: ExactPosition
position: 162
reference: Q00740-1

22852456756293678tt166
type: Position
type: ExactPosition
position: 166
reference: Q00740-1

22852456756293678tt169
type: Position
type: ExactPosition
position: 169
reference: Q00740-1

22852456756293678tt171
type: Position
type: ExactPosition
position: 171
reference: Q00740-1

22852456756293678tt186
type: Position
type: ExactPosition
position: 186
reference: Q00740-1

22852456756293678tt191
type: Position
type: ExactPosition
position: 191
reference: Q00740-1

22852456756293678tt200
type: Position
type: ExactPosition
position: 200
reference: Q00740-1

22852456756293678tt201
type: Position
type: ExactPosition
position: 201
reference: Q00740-1

22852456756293678tt211
type: Position
type: ExactPosition
position: 211
reference: Q00740-1

22852456756293678tt212
type: Position
type: ExactPosition
position: 212
reference: Q00740-1

22852456756293678tt214
type: Position
type: ExactPosition
position: 214
reference: Q00740-1

22852456756293678tt216
type: Position
type: ExactPosition
position: 216
reference: Q00740-1

22852456756293678tt218
type: Position
type: ExactPosition
position: 218
reference: Q00740-1

22852456756293678tt219
type: Position
type: ExactPosition
position: 219
reference: Q00740-1

22852456756293678tt223
type: Position
type: ExactPosition
position: 223
reference: Q00740-1

22852456756293678tt228
type: Position
type: ExactPosition
position: 228
reference: Q00740-1

22852456756293678tt246
type: Position
type: ExactPosition
position: 246
reference: Q00740-1

22852456756293678tt254
type: Position
type: ExactPosition
position: 254
reference: Q00740-1

22852456756293678tt256
type: Position
type: ExactPosition
position: 256
reference: Q00740-1

22852456756293678tt258
type: Position
type: ExactPosition
position: 258
reference: Q00740-1

22852456756293678tt260
type: Position
type: ExactPosition
position: 260
reference: Q00740-1

22852456756293678tt273
type: Position
type: ExactPosition
position: 273
reference: Q00740-1

22852456756293678tt286
type: Position
type: ExactPosition
position: 286
reference: Q00740-1

22852456756293678tt292
type: Position
type: ExactPosition
position: 292
reference: Q00740-1

22852456756293678tt294
type: Position
type: ExactPosition
position: 294
reference: Q00740-1

22852456756293678tt295
type: Position
type: ExactPosition
position: 295
reference: Q00740-1

22852456756293678tt307
type: Position
type: ExactPosition
position: 307
reference: Q00740-1

22852456756293678tt316
type: Position
type: ExactPosition
position: 316
reference: Q00740-1

22852456756293678tt321
type: Position
type: ExactPosition
position: 321
reference: Q00740-1

22852456756293678tt325
type: Position
type: ExactPosition
position: 325
reference: Q00740-1

22852456756293678tt331
type: Position
type: ExactPosition
position: 331
reference: Q00740-1

22852456756293678tt333
type: Position
type: ExactPosition
position: 333
reference: Q00740-1

22852456756293678tt335
type: Position
type: ExactPosition
position: 335
reference: Q00740-1

Q00740-1
type: Simple Sequence
modified: 1993-04-01
version: 1
mass: 36672
value: MNLQRFPRYPLTFGPTPIQPLARLSKHLGGKVHLYAKREDCNSGLAFGGNKTRKLEYLIPEALAQGCDTLVSIGGIQSNQTRQVAAVAAHLGMKCVLVQENWVNYSDAVYDRVGNIQMSRILGADVRLVPDGFDIGFRRSWEDALESVRAAGGKPYAIPAGCSDHPLGGLGFVGFAEEVRAQEAELGFKFDYVVVCSVTGSTQAGMVVGFAADGRADRVIGVDASAKPAQTREQITRIARQTAEKVGLERDIMRADVVLDERFAGPEYGLPNEGTLEAIRLCARTEGMLTDPVYEGKSMHGMIEMVRNGEFPEGSRVLYAHLGGVPALNGYSFIFRDG

http://purl.uniprot.org/uniprot/Q00740#attribution-EF49CFF7CB7DF66E7BB7BA4F7CE54F55
manual: true
evidence: ECO_0000255
source: MF_00807

http://purl.uniprot.org/uniprot/Q00740#attribution-0E16B74B1383D2C53B33CF8BBA9F02B8
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/1885510

P56653
type: Protein
reviewed: true
created: 1999-07-15
modified: 2023-05-03
version: 79
mnemonic: 4HBT_PSEUC
old mnemonic: 4HBT_PSESP
replaces: A5JTM7
citation: http://purl.uniprot.org/citations/1351742
citation: SIP1B3FB6BEB7CEBA2C
citation: http://purl.uniprot.org/citations/1610806
citation: http://purl.uniprot.org/citations/12220180
citation: http://purl.uniprot.org/citations/16962159
citation: http://purl.uniprot.org/citations/9837940
citation: http://purl.uniprot.org/citations/11997398
conflicting sequence: SIPC6EB3AC5AA69714D
recommended name: SIP4F1424A54E398A8E
enzyme: http://purl.uniprot.org/enzyme/3.1.2.23
organism: http://purl.uniprot.org/taxonomy/72586
annotation: SIP8AD48F5EAB20D83A
annotation: SIP7EC029B86F83FC6B
annotation: SIP365A9CBED74B94A1
annotation: SIP8C65E206B512DB76
annotation: SIPD187308FF169172E
annotation: SIPCBBACBE04C8801E7
annotation: SIP00E8E0121E3FE046
annotation: PRO_0000087760
annotation: SIPD65316FF484A22A0
annotation: SIP4A411906F1E3268D
annotation: SIP2C8EB2A6F8082768
annotation: SIPE376BAABB2E98989
annotation: SIPF59BCC1C6CE69682
annotation: SIPFAC0CEAE5AA03B9A
annotation: SIP10B38E7FCF2F514F
annotation: SIP82EAE4C53F0DCF84
annotation: SIPCF118F5728FB921A
annotation: SIPA7EF9EAE20498DAA
annotation: SIP9A0176507FDF4D8B
annotation: SIP3722DEE29EC1D16D
annotation: SIPAB818D637F2C130C
annotation: SIP9E917BCB46E3B4AC
annotation: SIP7BF377EEB9CF0F01
annotation: SIPB85F6FE2833CB11C
annotation: SIP2E2CD558358D0A8B
annotation: SIPD63573C3B3C8B4C9
annotation: SIP967EF7DD3DAF19E8
annotation: SIP7A12E0F23967A4B9
annotation: SIPF7BAC6DEEAD6F017
annotation: SIPA6A6F702FA30FCE2
annotation: SIPC359B66178CCA8B2
annotation: SIP3377C92C107E8F64
annotation: SIPCAA4ACB11954BC90
annotation: SIPD36C24D496365E55
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/2
classified with: http://purl.uniprot.org/keywords/378
classified with: GO_0018739
sequence: MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS

P56653-citation-1351742
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

SIP1B3FB6BEB7CEBA2C
type: Submission Citation
title: The nucleotide sequence upstream and downstream of 4-CBA-CoA ORFs in 9.5 kb Pseudomonas sp. strain CBS 3 chromosomal DNA.
author: Zhang W.
author: Dunaway-Mariano D.
date: 2007-04
submitted to: EMBL/GenBank/DDBJ

P56653-citation-SIP1B3FB6BEB7CEBA2C
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

P56653-citation-1610806
type: Citation Statement
scope: FUNCTION
scope: CATALYTIC ACTIVITY
scope: ACTIVITY REGULATION
scope: BIOPHYSICOCHEMICAL PROPERTIES
scope: PATHWAY
scope: SUBUNIT

P56653-citation-12220180
type: Citation Statement
scope: BIOPHYSICOCHEMICAL PROPERTIES
scope: MUTAGENESIS OF ASP-17; ARG-88; ARG-89; LYS-90; ARG-126 AND ARG-128

P56653-citation-16962159
type: Citation Statement
scope: BIOPHYSICOCHEMICAL PROPERTIES
scope: MUTAGENESIS OF ASP-32

P56653-citation-9837940
type: Citation Statement
scope: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS)
scope: SUBUNIT
scope: ACTIVE SITE

P56653-citation-11997398
type: Citation Statement
scope: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) OF WILD-TYPE IN COMPLEX WITH INHIBITOR AND MUTANT ASN-17 IN COMPLEX WITH SUBSTRATE
scope: SUBUNIT
scope: ACTIVE SITE

1BVQ
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.00
chain sequence mapping: PDB 1BVQ tt1tt141

PDB 1BVQ tt1tt141
chain: A=1-141

1LO7
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 1.50
chain sequence mapping: PDB 1LO7 tt1tt141

PDB 1LO7 tt1tt141
chain: A=1-141

1LO8
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 1.80
chain sequence mapping: PDB 1LO8 tt1tt141

PDB 1LO8 tt1tt141
chain: A=1-141

1LO9
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.80
chain sequence mapping: PDB 1LO9 tt1tt141

PDB 1LO9 tt1tt141
chain: A=1-141

DB01652
type: Resource
database: DrugBank
comment: 4-hydroxybenzoyl-CoA

DB04067
type: Resource
database: DrugBank
comment: 4-hydroxybenzyl coenzyme A

DB03613
type: Resource
database: DrugBank
comment: 4-hydroxyphenacyl coenzyme A

http://purl.uniprot.org/brenda/3.1.2.23
type: Resource
database: BRENDA
comment: 5085

cd00586
type: Resource
database: CDD
comment: 4HBT
signature sequence match: CDD cd00586 match 1

http://purl.uniprot.org/gene3d/3.10.129.10
type: Resource
database: Gene3D
comment: Hotdog Thioesterase
signature sequence match: Gene3D 3.10.129.10 match 1

IPR008272
type: Resource
database: InterPro
comment: HB-CoA_thioesterase_AS

IPR029069
type: Resource
database: InterPro
comment: HotDog_dom_sf

PF13279
type: Resource
database: Pfam
comment: 4HBT_2
signature sequence match: Pfam PF13279 match 1

SSF54637
type: Resource
database: SUPFAM
comment: Thioesterase/thiol ester dehydrase-isomerase
signature sequence match: SUPFAM SSF54637 match 1

PS01328
type: Resource
database: PROSITE
comment: 4HBCOA_THIOESTERASE
signature sequence match: PROSITE PS01328 match 1

SIP4F1424A54E398A8E
type: Structured Name
full name: 4-hydroxybenzoyl-CoA thioesterase
ec name: 3.1.2.23

SIP8AD48F5EAB20D83A
type: Function Annotation
comment: Hydrolyzes 4-hydroxybenzoate-CoA, and to a lesser extent benzoyl-CoA and 4-chlorobenzoate-CoA. Not active against aliphatic acyl-CoA thioesters, including palmitoyl-CoA, hexanoyl-CoA and acetyl-CoA.

SIP365A9CBED74B94A1
type: Activity Regulation Annotation
comment: Unaffected by EDTA, Mg(2+), Mn(2+), Fe(2+), Ca(2+), Co(2+) and Zn(2+).

SIP8C65E206B512DB76
type: Kinetics Annotation
measured affinity: 6 uM for 4-hydroxybenzoyl-CoA
measured affinity: 550 uM for 4-chlorobenzoyl-CoA
measured affinity: 200 uM for benzoyl-CoA
measured affinity: 270 uM for 4-hydroxybenzoyl-pantetheine
measured affinity: 56 uM for 4-methoxybenzoyl-CoA
measured affinity: 230 uM for 4-methylbenzoyl-CoA
measured affinity: 510 uM for benzoyl-CoA
measured affinity: 520 uM for 4-fluorobenzoyl-CoA
measured affinity: 300 uM for 4-trifluorobenzoyl-CoA

SIPCBBACBE04C8801E7
type: Subunit Annotation
comment: Homotetramer.

SIP00E8E0121E3FE046
type: Similarity Annotation
comment: Belongs to the 4-hydroxybenzoyl-CoA thioesterase family.

PRO_0000087760
type: Chain Annotation
comment: 4-hydroxybenzoyl-CoA thioesterase
mass: 16105
range: 22576505090814766tt1tt141

22576505090814766tt1
type: Position
type: ExactPosition
position: 1
reference: MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS

22576505090814766tt141
type: Position
type: ExactPosition
position: 141
reference: MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS

22576505090814766tt17
type: Position
type: ExactPosition
position: 17
reference: MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS

22576505090814766tt47
type: Position
type: ExactPosition
position: 47
reference: MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS

22576505090814766tt59
type: Position
type: ExactPosition
position: 59
reference: MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS

22576505090814766tt61
type: Position
type: ExactPosition
position: 61
reference: MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS

22576505090814766tt90
type: Position
type: ExactPosition
position: 90
reference: MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS

SIPF59BCC1C6CE69682
type: Mutagenesis Annotation
comment: Reduces catalytic activity. Little effect on substrate binding.
substitution: E
range: 22576505090814766tt17tt17

SIPFAC0CEAE5AA03B9A
type: Mutagenesis Annotation
comment: Drastically reduces catalytic activity. No effect on substrate binding.
substitution: N
range: 22576505090814766tt17tt17

SIP10B38E7FCF2F514F
type: Mutagenesis Annotation
comment: Drastically reduces catalytic activity.
substitution: S
range: 22576505090814766tt17tt17

SIP82EAE4C53F0DCF84
type: Mutagenesis Annotation
comment: Substrate turnover rate is decreased.
substitution: S
range: 22576505090814766tt32tt32

22576505090814766tt32
type: Position
type: ExactPosition
position: 32
reference: MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS

SIPCF118F5728FB921A
type: Mutagenesis Annotation
comment: No significant effect on catalytic activity or substrate binding.
substitution: A
range: 22576505090814766tt88tt88

22576505090814766tt88
type: Position
type: ExactPosition
position: 88
reference: MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS

SIPA7EF9EAE20498DAA
type: Mutagenesis Annotation
comment: No significant effect on catalytic activity or substrate binding.
substitution: L
range: 22576505090814766tt89tt89

22576505090814766tt89
type: Position
type: ExactPosition
position: 89
reference: MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS

SIP9A0176507FDF4D8B
type: Mutagenesis Annotation
comment: Decreases substrate binding affinity.
substitution: A
range: 22576505090814766tt90tt90

SIP3722DEE29EC1D16D
type: Mutagenesis Annotation
comment: No significant effect on catalytic activity or substrate binding.
substitution: L
range: 22576505090814766tt126tt126

22576505090814766tt126
type: Position
type: ExactPosition
position: 126
reference: MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS

SIPAB818D637F2C130C
type: Mutagenesis Annotation
comment: No significant effect on catalytic activity or substrate binding.
substitution: A
range: 22576505090814766tt128tt128

22576505090814766tt128
type: Position
type: ExactPosition
position: 128
reference: MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS

SIP9E917BCB46E3B4AC
type: Sequence Conflict Annotation
conflicting sequence: SIPC6EB3AC5AA69714D
substitution: L
range: 22576505090814766tt107tt107

22576505090814766tt107
type: Position
type: ExactPosition
position: 107
reference: MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS

22576505090814766tt4
type: Position
type: ExactPosition
position: 4
reference: MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS

22576505090814766tt10
type: Position
type: ExactPosition
position: 10
reference: MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS

22576505090814766tt13
type: Position
type: ExactPosition
position: 13
reference: MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS

22576505090814766tt15
type: Position
type: ExactPosition
position: 15
reference: MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS

22576505090814766tt20
type: Position
type: ExactPosition
position: 20
reference: MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS

22576505090814766tt22
type: Position
type: ExactPosition
position: 22
reference: MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS

22576505090814766tt25
type: Position
type: ExactPosition
position: 25
reference: MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS

22576505090814766tt41
type: Position
type: ExactPosition
position: 41
reference: MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS

22576505090814766tt54
type: Position
type: ExactPosition
position: 54
reference: MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS

22576505090814766tt56
type: Position
type: ExactPosition
position: 56
reference: MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS

22576505090814766tt58
type: Position
type: ExactPosition
position: 58
reference: MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS

22576505090814766tt68
type: Position
type: ExactPosition
position: 68
reference: MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS

22576505090814766tt77
type: Position
type: ExactPosition
position: 77
reference: MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS

22576505090814766tt87
type: Position
type: ExactPosition
position: 87
reference: MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS

22576505090814766tt101
type: Position
type: ExactPosition
position: 101
reference: MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS

22576505090814766tt123
type: Position
type: ExactPosition
position: 123
reference: MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS

22576505090814766tt130
type: Position
type: ExactPosition
position: 130
reference: MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS

22576505090814766tt134
type: Position
type: ExactPosition
position: 134
reference: MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS

22576505090814766tt140
type: Position
type: ExactPosition
position: 140
reference: MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS

substrate

MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS
type: Simple Sequence
modified: 1999-07-15
version: 1
mass: 16105

http://purl.uniprot.org/uniprot/P56653#attribution-70E422B2EA93C07430AB73C11927325A
creator: UniProtKB
manual: true
evidence: ECO_0000314
source: http://purl.uniprot.org/citations/1610806

http://purl.uniprot.org/uniprot/P56653#attribution-B62A1D4D8A501CB3F9944BCA9C033012
manual: true
evidence: ECO_0000255
source: PRU10041

http://purl.uniprot.org/uniprot/P56653#attribution-1EAF11C815DDE117761B11D6CF421F56
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/11997398

http://purl.uniprot.org/uniprot/P56653#attribution-A48373DBD8F124B96BBF17443754931F
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/12220180

http://purl.uniprot.org/uniprot/P56653#attribution-9BCC8A110D803976C46301E9195B55F9
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/1610806

http://purl.uniprot.org/uniprot/P56653#attribution-2ADFB8C22303EA635D1658F5D983D45A
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/16962159

http://purl.uniprot.org/uniprot/P56653#attribution-67C8DBA34598E7B1388A993336EF03DC
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/9837940

http://purl.uniprot.org/uniprot/P56653#attribution-080ED3982FEC43F7C43A0689717F3D20
manual: true
evidence: ECO_0000305

P49172
type: Protein
reviewed: true
created: 1996-02-01
modified: 2023-05-03
version: 96
mnemonic: 4OT_PSEUF
old mnemonic: 4OT_PSESP
old mnemonic: DMPI_PSEPU
old mnemonic: DMPI_PSESP
citation: http://purl.uniprot.org/citations/1732207
citation: http://purl.uniprot.org/citations/8547259
recommended name: SIP31C078FAE2095590
alternative name: SIPC1BAFE50128FA6FD
enzyme: http://purl.uniprot.org/enzyme/5.3.2.6
organism: http://purl.uniprot.org/taxonomy/79676
encoded in: pVI150
annotation: SIP5BFFEB0CF919391B
annotation: SIPA679D84CAD49ABE4
annotation: SIPA4B0262F890C6075
annotation: SIP868F6DE8089330A7
annotation: SIP335DB7F849FAC066
annotation: SIPA5627E035CDF8732
annotation: PRO_0000209513
annotation: SIPAE9FECDEB7E39322
annotation: SIPBBAC3140E0D4B5F5
annotation: SIP4D6E48954202BD08
annotation: SIPFBC98EDC37034F1A
annotation: SIP31751C4DF68F600A
annotation: SIP22BFFF8048F895A4
annotation: SIPB61E28D15534C79D
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/2
classified with: http://purl.uniprot.org/keywords/58
classified with: http://purl.uniprot.org/keywords/413
classified with: http://purl.uniprot.org/keywords/614
classified with: GO_0016853
classified with: GO_0042203
sequence: MPIAQLYIIEGRTDEQKETLIRQVSEAMANSLDAPLERVRVLITEMPKNHFGIGGEPASKVRR

P49172-citation-1732207
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

P49172-citation-8547259
type: Citation Statement
scope: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS)

1OTF
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 1.90
chain sequence mapping: PDB 1OTF tt2tt63

PDB 1OTF tt2tt63
chain: A/B/C/D/E/F=2-63

http://purl.uniprot.org/brenda/5.3.2.6
type: Resource
database: BRENDA
comment: 5085

cd00491
type: Resource
database: CDD
comment: 4Oxalocrotonate_Tautomerase
signature sequence match: CDD cd00491 match 1

http://purl.uniprot.org/gene3d/3.30.429.10
type: Resource
database: Gene3D
comment: Macrophage Migration Inhibitory Factor
signature sequence match: Gene3D 3.30.429.10 match 1

IPR004370
type: Resource
database: InterPro
comment: 4-oxalocrotonate_tautomerase

IPR014347
type: Resource
database: InterPro
comment: Tautomerase/MIF_sf

IPR018191
type: Resource
database: InterPro
comment: Tautomerase_Pseudo-typ

PTHR35530:SF1
type: Resource
database: PANTHER
comment: 2-HYDROXYMUCONATE TAUTOMERASE
signature sequence match: PANTHER PTHR35530:SF1 match 1

PTHR35530
type: Resource
database: PANTHER
comment: TAUTOMERASE-RELATED
signature sequence match: PANTHER PTHR35530 match 1

PF01361
type: Resource
database: Pfam
comment: Tautomerase
signature sequence match: Pfam PF01361 match 1

SSF55331
type: Resource
database: SUPFAM
comment: Tautomerase/MIF
signature sequence match: SUPFAM SSF55331 match 1

TIGR00013
type: Resource
database: TIGRFAMs
comment: taut
signature sequence match: TIGRFAMs TIGR00013 match 1

SIP31C078FAE2095590
type: Structured Name
full name: 2-hydroxymuconate tautomerase
ec name: 5.3.2.6

SIPC1BAFE50128FA6FD
type: Structured Name
full name: 4-oxalocrotonate tautomerase
short name: 4-OT

pVI150
type: Plasmid

dmpI
type: Gene

SIP5BFFEB0CF919391B
type: Function Annotation
comment: Catalyzes the ketonization of 2-hydroxymuconate stereoselectively to yield 2-oxo-3-hexenedioate.

SIP868F6DE8089330A7
type: Subunit Annotation
comment: Homohexamer.

SIP335DB7F849FAC066
type: Similarity Annotation
comment: Belongs to the 4-oxalocrotonate tautomerase family.

SIPA5627E035CDF8732
type: Initiator Methionine Annotation
comment: Removed
range: 22575418380333614tt1tt1

22575418380333614tt1
type: Position
type: ExactPosition
position: 1
reference: MPIAQLYIIEGRTDEQKETLIRQVSEAMANSLDAPLERVRVLITEMPKNHFGIGGEPASKVRR

PRO_0000209513
type: Chain Annotation
comment: 2-hydroxymuconate tautomerase
mass: 6974
range: 22575418380333614tt2tt63

22575418380333614tt2
type: Position
type: ExactPosition
position: 2
reference: MPIAQLYIIEGRTDEQKETLIRQVSEAMANSLDAPLERVRVLITEMPKNHFGIGGEPASKVRR

22575418380333614tt63
type: Position
type: ExactPosition
position: 63
reference: MPIAQLYIIEGRTDEQKETLIRQVSEAMANSLDAPLERVRVLITEMPKNHFGIGGEPASKVRR

SIPAE9FECDEB7E39322
type: Active Site Annotation
comment: Proton acceptor; via imino nitrogen
range: 22575418380333614tt2tt2

22575418380333614tt3
type: Position
type: ExactPosition
position: 3
reference: MPIAQLYIIEGRTDEQKETLIRQVSEAMANSLDAPLERVRVLITEMPKNHFGIGGEPASKVRR

22575418380333614tt10
type: Position
type: ExactPosition
position: 10
reference: MPIAQLYIIEGRTDEQKETLIRQVSEAMANSLDAPLERVRVLITEMPKNHFGIGGEPASKVRR

22575418380333614tt14
type: Position
type: ExactPosition
position: 14
reference: MPIAQLYIIEGRTDEQKETLIRQVSEAMANSLDAPLERVRVLITEMPKNHFGIGGEPASKVRR

22575418380333614tt32
type: Position
type: ExactPosition
position: 32
reference: MPIAQLYIIEGRTDEQKETLIRQVSEAMANSLDAPLERVRVLITEMPKNHFGIGGEPASKVRR

22575418380333614tt36
type: Position
type: ExactPosition
position: 36
reference: MPIAQLYIIEGRTDEQKETLIRQVSEAMANSLDAPLERVRVLITEMPKNHFGIGGEPASKVRR

22575418380333614tt38
type: Position
type: ExactPosition
position: 38
reference: MPIAQLYIIEGRTDEQKETLIRQVSEAMANSLDAPLERVRVLITEMPKNHFGIGGEPASKVRR

22575418380333614tt40
type: Position
type: ExactPosition
position: 40
reference: MPIAQLYIIEGRTDEQKETLIRQVSEAMANSLDAPLERVRVLITEMPKNHFGIGGEPASKVRR

22575418380333614tt46
type: Position
type: ExactPosition
position: 46
reference: MPIAQLYIIEGRTDEQKETLIRQVSEAMANSLDAPLERVRVLITEMPKNHFGIGGEPASKVRR

22575418380333614tt48
type: Position
type: ExactPosition
position: 48
reference: MPIAQLYIIEGRTDEQKETLIRQVSEAMANSLDAPLERVRVLITEMPKNHFGIGGEPASKVRR

22575418380333614tt50
type: Position
type: ExactPosition
position: 50
reference: MPIAQLYIIEGRTDEQKETLIRQVSEAMANSLDAPLERVRVLITEMPKNHFGIGGEPASKVRR

22575418380333614tt51
type: Position
type: ExactPosition
position: 51
reference: MPIAQLYIIEGRTDEQKETLIRQVSEAMANSLDAPLERVRVLITEMPKNHFGIGGEPASKVRR

22575418380333614tt53
type: Position
type: ExactPosition
position: 53
reference: MPIAQLYIIEGRTDEQKETLIRQVSEAMANSLDAPLERVRVLITEMPKNHFGIGGEPASKVRR

MPIAQLYIIEGRTDEQKETLIRQVSEAMANSLDAPLERVRVLITEMPKNHFGIGGEPASKVRR
type: Simple Sequence
modified: 2007-01-23
version: 2
mass: 7105

http://purl.uniprot.org/uniprot/P49172#attribution-609619A7DF3E53CE5E48EDEF8B8F9DD1
manual: true
evidence: ECO_0000250

http://purl.uniprot.org/uniprot/P49172#attribution-080ED3982FEC43F7C43A0689717F3D20
manual: true
evidence: ECO_0000305

Q52060
type: Protein
reviewed: true
created: 2011-02-08
modified: 2023-05-03
version: 101
mnemonic: ACDH_PSEUF
citation: http://purl.uniprot.org/citations/1732207
citation: http://purl.uniprot.org/citations/8419288
citation: http://purl.uniprot.org/citations/12764229
recommended name: SIPC3C24560DD252485
alternative name: SIP116DF1E75A719A69
enzyme: http://purl.uniprot.org/enzyme/1.2.1.10
organism: http://purl.uniprot.org/taxonomy/79676
encoded in: pVI150
annotation: SIP0515E0E80A6B39EB
annotation: SIPBD4C15B79DFAA551
annotation: SIPAF74C3C336094E34
annotation: SIP1D401DD7C8555AAA
annotation: SIPE79D3B1755FB87A8
annotation: SIP058ABD837794DB8F
annotation: SIPD958E4C428C4B4D9
annotation: PRO_0000404201
annotation: SIPF0C213D977B6D1AE
annotation: SIP73C2C0826E3680E3
annotation: SIPA64B65308E6E786D
annotation: SIPF6F086DE5A5172CA
annotation: SIPEE5BDBB326875295
annotation: SIP3E6FC8C376FBE3BD
annotation: SIP03805FC44CAA9BED
annotation: SIP6850177D3525EAA4
annotation: SIPC0AF7FBEA376C64B
annotation: SIP6B777DD732424B3B
annotation: SIPDDCB6268F0851E9B
annotation: SIP31709DCDE0D3A06F
annotation: SIP33E16B39E0A613DE
annotation: SIPC61139DDC156AED7
annotation: SIP48840AAEC49D9818
annotation: SIP2390A9F6180EF002
annotation: SIP9D11158043BAE0D8
annotation: SIPB2F2B626D4F9630A
annotation: SIP6EFC4BC4A2A3D3E5
annotation: SIPA11BF2F1BF5C856F
annotation: SIP62FD9B13EFE48A8C
annotation: SIP03AAA13BA2E43ADC
annotation: SIP7CF2A722485FF01D
annotation: SIPE110FDD49DCC43BF
annotation: SIP42A604BFD90A39B0
annotation: SIP918A66E9783FF804
annotation: SIPFE2AF5264E3F4A55
annotation: SIPA1168C2E352F5CCE
annotation: SIPC83113A0A78BE9F7
annotation: SIPEBED6A9513C9C001
annotation: SIPDAEEC58E55108BE0
annotation: SIP792E37F5592B2424
annotation: SIP3277BC31D8774356
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/2
classified with: http://purl.uniprot.org/keywords/58
classified with: http://purl.uniprot.org/keywords/903
classified with: http://purl.uniprot.org/keywords/520
classified with: http://purl.uniprot.org/keywords/560
classified with: http://purl.uniprot.org/keywords/614
classified with: GO_0008774
classified with: GO_0051287
classified with: GO_0050661
classified with: GO_0019439
classified with: GO_0019336
sequence: Q52060-1

Q52060-citation-1732207
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
scope: FUNCTION

CF600
type: Strain

Q52060-citation-8419288
type: Citation Statement
scope: PROTEIN SEQUENCE OF 1-6
scope: CATALYTIC ACTIVITY
scope: SUBSTRATE SPECIFICITY
scope: ACTIVITY REGULATION
scope: SUBUNIT
scope: INTERACTION WITH DMPG
scope: PH DEPENDENCE

Q52060-citation-12764229
type: Citation Statement
scope: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) IN COMPLEX WITH DMPG AND NAD
scope: SUBUNIT
scope: REACTION MECHANISM

1NVM
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 1.70
chain sequence mapping: PDB 1NVM tt1tt312
chain sequence mapping: PDB 1NVM tt1tt345

PDB 1NVM tt1tt312
chain: B/D/F/H=1-312

Q52060
type: Resource
database: IntAct
comment: 1

http://purl.uniprot.org/brenda/1.2.1.10
type: Resource
database: BRENDA
comment: 5085

http://purl.uniprot.org/gene3d/3.40.50.720
type: Resource
database: Gene3D
comment: NAD(P)-binding Rossmann-like Domain
signature sequence match: Gene3D 3.40.50.720 match 1
signature sequence match: Gene3D 3.40.50.720 match 2

MF_01657
type: Resource
database: HAMAP
comment: Ac_ald_DH_ac
signature sequence match: HAMAP MF 01657 match 1

IPR003361
type: Resource
database: InterPro
comment: Acetaldehyde_dehydrogenase

IPR015426
type: Resource
database: InterPro
comment: Acetylaldehyde_DH_C

IPR036291
type: Resource
database: InterPro
comment: NAD(P)-bd_dom_sf

IPR000534
type: Resource
database: InterPro
comment: Semialdehyde_DH_NAD-bd

PF09290
type: Resource
database: Pfam
comment: AcetDehyd-dimer
signature sequence match: Pfam PF09290 match 1

PF01118
type: Resource
database: Pfam
comment: Semialdhyde_dh
signature sequence match: Pfam PF01118 match 1

PIRSF015689
type: Resource
database: PIRSF
comment: Actaldh_dh_actl
signature sequence match: PIRSF PIRSF015689 match 1

SM00859
type: Resource
database: SMART
comment: Semialdhyde_dh
signature sequence match: SMART SM00859 match 1

SSF55347
type: Resource
database: SUPFAM
comment: Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain
signature sequence match: SUPFAM SSF55347 match 1

SSF51735
type: Resource
database: SUPFAM
comment: NAD(P)-binding Rossmann-fold domains
signature sequence match: SUPFAM SSF51735 match 1

TIGR03215
type: Resource
database: TIGRFAMs
comment: ac_ald_DH_ac
signature sequence match: TIGRFAMs TIGR03215 match 1

SIPC3C24560DD252485
type: Structured Name
full name: Acetaldehyde dehydrogenase
ec name: 1.2.1.10

SIP116DF1E75A719A69
type: Structured Name
full name: Acetaldehyde dehydrogenase [acetylating]

pVI150
type: Plasmid

dmpF
type: Gene

SIP0515E0E80A6B39EB
type: Function Annotation
comment: Catalyzes the conversion of acetaldehyde to acetyl-CoA, using NAD(+) and coenzyme A. Can also act on propanal and butanal to form propanoyl-CoA and butanoyl-CoA, respectively. Is the final enzyme in the meta-cleavage pathway for the degradation of aromatic compounds such as phenols, cresols and catechols. NADP(+) can replace NAD(+) but the rate of reaction is much slower.

SIPAF74C3C336094E34
type: Activity Regulation Annotation
comment: Is not activated by Mn(2+), Mg(2+), Ca(2+), Zn(2+) or Co(2+).

SIP1D401DD7C8555AAA
type: PH Dependence Annotation
comment: Activity increases gradually over the pH range 6.5-8.5.

SIP058ABD837794DB8F
type: Subunit Annotation
comment: Heterotetramer composed of two DmpG (aldolase) and two DmpF (dehydrogenase) subunits, which allows a direct channeling of acetaldehyde between the two active sites.

SIPD958E4C428C4B4D9
type: Similarity Annotation
comment: Belongs to the acetaldehyde dehydrogenase family.

PRO_0000404201
type: Chain Annotation
comment: Acetaldehyde dehydrogenase
mass: 32685
range: 22857962787057710tt1tt312

22857962787057710tt1
type: Position
type: ExactPosition
position: 1
reference: Q52060-1

22857962787057710tt312
type: Position
type: ExactPosition
position: 312
reference: Q52060-1

SIPF0C213D977B6D1AE
type: Active Site Annotation
comment: Acyl-thioester intermediate
range: 22857962787057710tt132tt132

22857962787057710tt132
type: Position
type: ExactPosition
position: 132
reference: Q52060-1

22857962787057710tt12
type: Position
type: ExactPosition
position: 12
reference: Q52060-1

22857962787057710tt15
type: Position
type: ExactPosition
position: 15
reference: Q52060-1

22857962787057710tt163
type: Position
type: ExactPosition
position: 163
reference: Q52060-1

22857962787057710tt171
type: Position
type: ExactPosition
position: 171
reference: Q52060-1

22857962787057710tt290
type: Position
type: ExactPosition
position: 290
reference: Q52060-1

22857962787057710tt5
type: Position
type: ExactPosition
position: 5
reference: Q52060-1

22857962787057710tt10
type: Position
type: ExactPosition
position: 10
reference: Q52060-1

22857962787057710tt14
type: Position
type: ExactPosition
position: 14
reference: Q52060-1

22857962787057710tt26
type: Position
type: ExactPosition
position: 26
reference: Q52060-1

22857962787057710tt28
type: Position
type: ExactPosition
position: 28
reference: Q52060-1

22857962787057710tt36
type: Position
type: ExactPosition
position: 36
reference: Q52060-1

22857962787057710tt43
type: Position
type: ExactPosition
position: 43
reference: Q52060-1

22857962787057710tt50
type: Position
type: ExactPosition
position: 50
reference: Q52060-1

22857962787057710tt55
type: Position
type: ExactPosition
position: 55
reference: Q52060-1

22857962787057710tt58
type: Position
type: ExactPosition
position: 58
reference: Q52060-1

22857962787057710tt59
type: Position
type: ExactPosition
position: 59
reference: Q52060-1

22857962787057710tt65
type: Position
type: ExactPosition
position: 65
reference: Q52060-1

22857962787057710tt67
type: Position
type: ExactPosition
position: 67
reference: Q52060-1

22857962787057710tt71
type: Position
type: ExactPosition
position: 71
reference: Q52060-1

22857962787057710tt72
type: Position
type: ExactPosition
position: 72
reference: Q52060-1

22857962787057710tt77
type: Position
type: ExactPosition
position: 77
reference: Q52060-1

22857962787057710tt81
type: Position
type: ExactPosition
position: 81
reference: Q52060-1

22857962787057710tt94
type: Position
type: ExactPosition
position: 94
reference: Q52060-1

22857962787057710tt99
type: Position
type: ExactPosition
position: 99
reference: Q52060-1

22857962787057710tt102
type: Position
type: ExactPosition
position: 102
reference: Q52060-1

22857962787057710tt114
type: Position
type: ExactPosition
position: 114
reference: Q52060-1

22857962787057710tt117
type: Position
type: ExactPosition
position: 117
reference: Q52060-1

22857962787057710tt118
type: Position
type: ExactPosition
position: 118
reference: Q52060-1

22857962787057710tt123
type: Position
type: ExactPosition
position: 123
reference: Q52060-1

22857962787057710tt125
type: Position
type: ExactPosition
position: 125
reference: Q52060-1

22857962787057710tt128
type: Position
type: ExactPosition
position: 128
reference: Q52060-1

22857962787057710tt145
type: Position
type: ExactPosition
position: 145
reference: Q52060-1

22857962787057710tt150
type: Position
type: ExactPosition
position: 150
reference: Q52060-1

22857962787057710tt160
type: Position
type: ExactPosition
position: 160
reference: Q52060-1

22857962787057710tt161
type: Position
type: ExactPosition
position: 161
reference: Q52060-1

22857962787057710tt166
type: Position
type: ExactPosition
position: 166
reference: Q52060-1

22857962787057710tt169
type: Position
type: ExactPosition
position: 169
reference: Q52060-1

22857962787057710tt172
type: Position
type: ExactPosition
position: 172
reference: Q52060-1

22857962787057710tt185
type: Position
type: ExactPosition
position: 185
reference: Q52060-1

22857962787057710tt190
type: Position
type: ExactPosition
position: 190
reference: Q52060-1

22857962787057710tt199
type: Position
type: ExactPosition
position: 199
reference: Q52060-1

22857962787057710tt207
type: Position
type: ExactPosition
position: 207
reference: Q52060-1

22857962787057710tt217
type: Position
type: ExactPosition
position: 217
reference: Q52060-1

22857962787057710tt220
type: Position
type: ExactPosition
position: 220
reference: Q52060-1

22857962787057710tt235
type: Position
type: ExactPosition
position: 235
reference: Q52060-1

22857962787057710tt241
type: Position
type: ExactPosition
position: 241
reference: Q52060-1

22857962787057710tt245
type: Position
type: ExactPosition
position: 245
reference: Q52060-1

22857962787057710tt248
type: Position
type: ExactPosition
position: 248
reference: Q52060-1

22857962787057710tt252
type: Position
type: ExactPosition
position: 252
reference: Q52060-1

22857962787057710tt258
type: Position
type: ExactPosition
position: 258
reference: Q52060-1

22857962787057710tt260
type: Position
type: ExactPosition
position: 260
reference: Q52060-1

22857962787057710tt261
type: Position
type: ExactPosition
position: 261
reference: Q52060-1

22857962787057710tt263
type: Position
type: ExactPosition
position: 263
reference: Q52060-1

22857962787057710tt264
type: Position
type: ExactPosition
position: 264
reference: Q52060-1

22857962787057710tt266
type: Position
type: ExactPosition
position: 266
reference: Q52060-1

22857962787057710tt268
type: Position
type: ExactPosition
position: 268
reference: Q52060-1

22857962787057710tt277
type: Position
type: ExactPosition
position: 277
reference: Q52060-1

22857962787057710tt282
type: Position
type: ExactPosition
position: 282
reference: Q52060-1

22857962787057710tt284
type: Position
type: ExactPosition
position: 284
reference: Q52060-1

22857962787057710tt289
type: Position
type: ExactPosition
position: 289
reference: Q52060-1

22857962787057710tt310
type: Position
type: ExactPosition
position: 310
reference: Q52060-1

Q52060-1
type: Simple Sequence
modified: 1996-11-01
version: 1
mass: 32685
value: MNQKLKVAIIGSGNIGTDLMIKVLRNAKYLEMGAMVGIDAASDGLARAQRMGVTTTYAGVEGLIKLPEFADIDFVFDATSASAHVQNEALLRQAKPGIRLIDLTPAAIGPYCVPVVNLEEHLGKLNVNMVTCGGQATIPMVAAVSRVAKVHYAEIVASISSKSAGPGTRANIDEFTETTSKAIEVIGGAAKGKAIIIMNPAEPPLIMRDTVYVLSAAADQAAVAASVAEMVQAVQAYVPGYRLKQQVQFDVIPESAPLNIPGLGRFSGLKTSVFLEVEGAAHYLPAYAGNLDIMTSAALATAERMAQSMLNA

http://purl.uniprot.org/uniprot/Q52060#attribution-44F726B6481B3054005E8DE690D0A2B6
creator: UniProtKB
manual: true
evidence: ECO_0000314
source: http://purl.uniprot.org/citations/8419288

http://purl.uniprot.org/uniprot/Q52060#attribution-A474DB4F1AC35E93635077AA1A324865
creator: UniProtKB
manual: true
evidence: ECO_0000314
source: http://purl.uniprot.org/citations/12764229

http://purl.uniprot.org/uniprot/Q52060#attribution-F248E7B692F3F3A167EED240EFCEF57F
manual: true
evidence: ECO_0000255
source: MF_01657

http://purl.uniprot.org/uniprot/Q52060#attribution-2896F89442AE0BAB4AA8721FBD8C1C43
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/12764229

http://purl.uniprot.org/uniprot/Q52060#attribution-5BE3D9FC39CCB10D8A4B1CB4C5034062
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/1732207

http://purl.uniprot.org/uniprot/Q52060#attribution-09B7B99FA8656CCA3E840206F13D3A3B
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/8419288

http://purl.uniprot.org/uniprot/Q52060#attribution-080ED3982FEC43F7C43A0689717F3D20
manual: true
evidence: ECO_0000305

Q06365
type: Protein
reviewed: true
created: 1998-12-15
modified: 2023-05-03
version: 71
mnemonic: ALYP_PSESO
citation: http://purl.uniprot.org/citations/8336113
recommended name: SIP6312F6455255EC4D
alternative name: SIP7C24EF6D04A27264
alternative name: SIPFE005BE86183C4F2
enzyme: http://purl.uniprot.org/enzyme/4.2.2.3
organism: http://purl.uniprot.org/taxonomy/86038
annotation: SIP0F9828FDDF10AC41
annotation: SIPAFBEAC7865D5EDE6
annotation: SIP1DA8836C6EB06127
annotation: PRO_0000024924
existence: Inferred from Homology Existence
classified with: http://purl.uniprot.org/keywords/456
classified with: http://purl.uniprot.org/keywords/732
classified with: GO_0045135
sequence: Q06365-1

Q06365-citation-8336113
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

PL6
type: Resource
database: CAZy
comment: Polysaccharide Lyase Family 6

cd14251
type: Resource
database: CDD
comment: PL-6
signature sequence match: CDD cd14251 match 1

http://purl.uniprot.org/gene3d/2.160.20.10
type: Resource
database: Gene3D
comment: Single-stranded right-handed beta-helix, Pectin lyase-like
signature sequence match: Gene3D 2.160.20.10 match 1

IPR012334
type: Resource
database: InterPro
comment: Pectin_lyas_fold

IPR011050
type: Resource
database: InterPro
comment: Pectin_lyase_fold/virulence

IPR039513
type: Resource
database: InterPro
comment: PL-6

PF14592
type: Resource
database: Pfam
comment: Chondroitinas_B
signature sequence match: Pfam PF14592 match 1

SSF51126
type: Resource
database: SUPFAM
comment: Pectin lyase-like
signature sequence match: SUPFAM SSF51126 match 1

SIP6312F6455255EC4D
type: Structured Name
full name: Alginate lyase
ec name: 4.2.2.3

SIP7C24EF6D04A27264
type: Structured Name
full name: Poly(beta-D-mannuronate) lyase

SIPFE005BE86183C4F2
type: Structured Name
full name: Poly(mana) alginate lyase

aly
type: Gene
alternative label: alyP

SIPAFBEAC7865D5EDE6
type: Similarity Annotation
comment: Belongs to the polysaccharide lyase 6 family.

22852482459120942tt1
type: Position
type: ExactPosition
position: 1
reference: Q06365-1

PRO_0000024924
type: Chain Annotation
comment: Alginate lyase
mass: 44512
range: 22852482459120942nbtt398

22852482459120942tt398
type: Position
type: ExactPosition
position: 398
reference: Q06365-1

Q06365-1
type: Simple Sequence
modified: 1996-11-01
version: 1
precursor: true
mass: 44512
value: MPGDKVIMKSGEWKSQFIHFKGKGTAEKPITLTAETKGSVLLTGNSNLKIDGEWLVVDGLSFKNGFSLKDDVVVFTKTTTNSRLTNTSIENYNPVDKTLDYKWVSLYGHHNRVDHCSITGKNHQGTTLVVWLDDKPNYHQIDHNYFGPRPELGANGGETIRIGTSAFSMNDSYRTVQNNIFDKCDGEVEIISIKSGFNKILNNLFYECAGTVTFRHGNNSEVSNNYFIANNVTNSGGVRIIGENQKVYGNYLYKVAGRTLRSAISVMNAYEKPALNDYWQVKNADIQNNIIVGAREAFVLGSGKDNDRTLAPDGVNISNNYIINPTTLLVTQDEPKNLKMQNNQVEGASIVTGFVKMGNDLQMSDGIWQKKTEIKKPFWLATAIGPEWKKDHRSFIFK

http://purl.uniprot.org/uniprot/Q06365#attribution-DC036A124EC8E88DF072B29F6D9BB9FE
manual: true
evidence: ECO_0000255

http://purl.uniprot.org/uniprot/Q06365#attribution-080ED3982FEC43F7C43A0689717F3D20
manual: true
evidence: ECO_0000305

P0A2Y4
type: Protein
reviewed: true
created: 2005-03-15
modified: 2023-05-03
version: 71
mnemonic: AROA_PSES2
replaces: P56952
citation: SIP35597ACFE246013D
recommended name: SIP9B7B8EEDD6AC7691
alternative name: SIP7BCBDA8035E4F966
enzyme: http://purl.uniprot.org/enzyme/2.5.1.19
organism: http://purl.uniprot.org/taxonomy/308
annotation: SIP5ED583DB6730D48C
annotation: SIP24846A69D61BE2EB
annotation: SIPD2122A9173D4BA3A
annotation: SIP613165B98C268932
annotation: SIPD854721C1B6F9840
annotation: SIP96A3AB72C8AA8395
annotation: SIPDBE294CD578ED56C
annotation: PRO_0000088280
annotation: SIP6E95F9058123A812
annotation: SIP64E8ED84288A7333
annotation: SIPA2140D079E8EAD1D
annotation: SIP0A2DE141DFBA38C9
annotation: SIP337F931FEE0963A3
annotation: SIP1B01616C5EC17239
annotation: SIP08E808DE4CC63E69
annotation: SIP5CDC350B74DB7FDB
annotation: SIPBE8410FB3EFAF6E4
annotation: SIPA8B3CA58814DC386
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/57
classified with: http://purl.uniprot.org/keywords/963
classified with: http://purl.uniprot.org/keywords/903
classified with: http://purl.uniprot.org/keywords/359
classified with: http://purl.uniprot.org/keywords/808
classified with: GO_0005737
classified with: GO_0003866
classified with: GO_0008652
classified with: GO_0009073
classified with: GO_0009423
classified with: GO_0009635
sequence: P0A2Y4-1

SIP35597ACFE246013D
type: Patent Citation
title: Glyphosate-tolerant 5-enolpyruvylshikimate-3-phosphate synthases.
author: Barry G.F.
author: Kishore G.M.
author: Padgette S.R.
author: Stallings W.C.
date: 1997-05-27

P0A2Y4-citation-SIP35597ACFE246013D
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
scope: PROTEIN SEQUENCE OF 2-16

cd01556
type: Resource
database: CDD
comment: EPSP_synthase
signature sequence match: CDD cd01556 match 1

http://purl.uniprot.org/gene3d/3.65.10.10
type: Resource
database: Gene3D
comment: Enolpyruvate transferase domain
signature sequence match: Gene3D 3.65.10.10 match 1
signature sequence match: Gene3D 3.65.10.10 match 2

MF_00210
type: Resource
database: HAMAP
comment: EPSP_synth
signature sequence match: HAMAP MF 00210 match 1

IPR001986
type: Resource
database: InterPro
comment: Enolpyruvate_Tfrase_dom

IPR036968
type: Resource
database: InterPro
comment: Enolpyruvate_Tfrase_sf

IPR006264
type: Resource
database: InterPro
comment: EPSP_synthase

IPR023193
type: Resource
database: InterPro
comment: EPSP_synthase_CS

IPR013792
type: Resource
database: InterPro
comment: RNA3'P_cycl/enolpyr_Trfase_a/b

PTHR21090
type: Resource
database: PANTHER
comment: AROM/DEHYDROQUINATE SYNTHASE
signature sequence match: PANTHER PTHR21090 match 1

PTHR21090:SF5
type: Resource
database: PANTHER
comment: PENTAFUNCTIONAL AROM POLYPEPTIDE
signature sequence match: PANTHER PTHR21090:SF5 match 1

PF00275
type: Resource
database: Pfam
comment: EPSP_synthase
signature sequence match: Pfam PF00275 match 1

PIRSF000505
type: Resource
database: PIRSF
comment: EPSPS
signature sequence match: PIRSF PIRSF000505 match 1

SSF55205
type: Resource
database: SUPFAM
comment: EPT/RTPC-like
signature sequence match: SUPFAM SSF55205 match 1

TIGR01356
type: Resource
database: TIGRFAMs
comment: aroA
signature sequence match: TIGRFAMs TIGR01356 match 1

PS00104
type: Resource
database: PROSITE
comment: EPSP_SYNTHASE_1
signature sequence match: PROSITE PS00104 match 1

PS00885
type: Resource
database: PROSITE
comment: EPSP_SYNTHASE_2
signature sequence match: PROSITE PS00885 match 1

SIP9B7B8EEDD6AC7691
type: Structured Name
full name: 3-phosphoshikimate 1-carboxyvinyltransferase
ec name: 2.5.1.19

SIP7BCBDA8035E4F966
type: Structured Name
full name: 5-enolpyruvylshikimate-3-phosphate synthase
short name: EPSP synthase
short name: EPSPS

aroA
type: Gene

SIP5ED583DB6730D48C
type: Function Annotation
comment: Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate.

SIP613165B98C268932
type: Subunit Annotation
comment: Monomer.

SIP96A3AB72C8AA8395
type: Annotation
comment: Resistant to the antibiotic glyphosate.

SIPDBE294CD578ED56C
type: Similarity Annotation
comment: Belongs to the EPSP synthase family.

PRO_0000088280
type: Chain Annotation
comment: 3-phosphoshikimate 1-carboxyvinyltransferase
mass: 47297
range: 22571054712566830tt1tt449

22571054712566830tt1
type: Position
type: ExactPosition
position: 1
reference: P0A2Y4-1

22571054712566830tt449
type: Position
type: ExactPosition
position: 449
reference: P0A2Y4-1

SIP6E95F9058123A812
type: Region Annotation
comment: Disordered
range: 22571054712566830tt1tt23

22571054712566830tt23
type: Position
type: ExactPosition
position: 23
reference: P0A2Y4-1

SIP64E8ED84288A7333
type: Region Annotation
comment: Phosphoenolpyruvate
range: 22571054712566830tt98tt101

22571054712566830tt98
type: Position
type: ExactPosition
position: 98
reference: P0A2Y4-1

22571054712566830tt101
type: Position
type: ExactPosition
position: 101
reference: P0A2Y4-1

SIPA2140D079E8EAD1D
type: Active Site Annotation
comment: Proton acceptor
range: 22571054712566830tt326tt326

22571054712566830tt326
type: Position
type: ExactPosition
position: 326
reference: P0A2Y4-1

SIP0A2DE141DFBA38C9
type: Active Site Annotation
comment: Proton donor
range: 22571054712566830tt354tt354

22571054712566830tt354
type: Position
type: ExactPosition
position: 354
reference: P0A2Y4-1

22571054712566830tt28
type: Position
type: ExactPosition
position: 28
reference: P0A2Y4-1

22571054712566830tt29
type: Position
type: ExactPosition
position: 29
reference: P0A2Y4-1

22571054712566830tt33
type: Position
type: ExactPosition
position: 33
reference: P0A2Y4-1

22571054712566830tt128
type: Position
type: ExactPosition
position: 128
reference: P0A2Y4-1

22571054712566830tt353
type: Position
type: ExactPosition
position: 353
reference: P0A2Y4-1

22571054712566830tt357
type: Position
type: ExactPosition
position: 357
reference: P0A2Y4-1

22571054712566830tt402
type: Position
type: ExactPosition
position: 402
reference: P0A2Y4-1

P0A2Y4-1
type: Simple Sequence
modified: 2005-03-15
version: 1
mass: 47297
value: MSHSASPKPATARRSEALTGEIRIPGDKSISHRSFMFGGLASGETRITGLLEGEDVINTGRAMQAMGAKIRKEGDVWIINGVGNGCLLQPEAALDFGNAGTGARLTMGLVGTYDMKTSFIGDASLSKRPMGRVLNPLREMGVQVEAADGDRMPLTLIGPKTANPITYRVPMASAQVKSAVLLAGLNTPGVTTVIEPVMTRDHTEKMLQGFGADLTVETDKDGVRHIRITGQGKLVGQTIDVPGDPSSTAFPLVAALLVEGSDVTIRNVLMNPTRTGLILTLQEMGADIEVLNARLAGGEDVADLRVRASKLKGVVVPPERAPSMIDEYPVLAIAASFAEGETVMDGLDELRVKESDRLAAVARGLEANGVDCTEGEMSLTVRGRPDGKGLGGGTVATHLDHRIAMSFLVMGLAAEKPVTVDDSNMIATSFPEFMDMMPGLGAKIELSIL

http://purl.uniprot.org/uniprot/P0A2Y4#attribution-0A561F4F067256E9CA6406750D5E4A5F
manual: true
evidence: ECO_0000255
source: MF_00210

http://purl.uniprot.org/uniprot/P0A2Y4#attribution-080ED3982FEC43F7C43A0689717F3D20
manual: true
evidence: ECO_0000305

P10182
type: Protein
reviewed: true
created: 1989-07-01
modified: 2023-05-03
version: 101
mnemonic: ASPQ_PSES7
old mnemonic: ASPQ_PSESP
citation: http://purl.uniprot.org/citations/8068664
citation: http://purl.uniprot.org/citations/619999
citation: http://purl.uniprot.org/citations/9020792
citation: http://purl.uniprot.org/citations/10684596
conflicting sequence: SIPC8BC812CE7E9D4B4
recommended name: SIPD87756D7FEBCFCFD
alternative name: SIP753887D11BCB0194
alternative name: SIP2CCF2CFF0992EA89
enzyme: http://purl.uniprot.org/enzyme/3.5.1.38
organism: http://purl.uniprot.org/taxonomy/313
annotation: SIPE8F6A7881E874169
annotation: SIPA74DBC5428ABAB2D
annotation: SIP53D6978A1B0ED1DE
annotation: SIPC45A5B8CFC10BE43
annotation: SIPF41BA78C1170023A
annotation: PRO_0000171089
annotation: SIPB65FD4840891480D
annotation: SIP01B9D40BB725A062
annotation: SIP4ADB3D389944403C
annotation: SIP0F14209CFC3A5526
annotation: SIP16B8DB373625623F
annotation: SIPE26164CA2452FD80
annotation: SIP21100A7DC69D6870
annotation: SIPA438626B6144E533
annotation: SIPA7DD44088150A537
annotation: SIP1C25B4A12D48975B
annotation: SIP2A5719BCE8512E26
annotation: SIP5A3688C84BAC259C
annotation: SIPE0387D47B484ED36
annotation: SIPD3DFB2E7484B4B3A
annotation: SIP38DF699E132F5C74
annotation: SIP69323C6D698030C1
annotation: SIPD415F3328CE7C39D
annotation: SIP0E2978DBBCA31ECF
annotation: SIPD1BF11C9C90853F4
annotation: SIP4D47111D9F9B46D6
annotation: SIP9DB6482FC3B4ACE0
annotation: SIPA9A3B7D96748433C
annotation: SIP4DAEF5E6FD9ACA47
annotation: SIPB16186B5C70BB5F2
annotation: SIPF94F3C42274E40A3
annotation: SIP4EAF1AD6AFF48347
annotation: SIP34E9AC92D3C94A73
annotation: SIP643A6E97B0AAC4C9
annotation: SIPDA1D3CC6311900BF
annotation: SIPD338AC0E970B8374
annotation: SIP9C29B5CF6F284B3A
annotation: SIP47E7B11663B43D23
annotation: SIP0109FB2D7AB3099A
annotation: SIP1B3FDB572C67F4AF
annotation: SIPD3953690E0B87EF1
annotation: SIPE847BFA255507A1C
annotation: SIP39E699654643E7CE
annotation: SIP71726FDDE3DFB30D
annotation: SIP82B0EE58F4A38362
annotation: SIPB209B8797EADC3F4
annotation: SIPFD95ACA79001C8CF
annotation: SIP9C0E073CB4E7792F
annotation: SIP34115368E348747C
annotation: SIPED3BC7643CD2CF82
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/2
classified with: http://purl.uniprot.org/keywords/903
classified with: http://purl.uniprot.org/keywords/378
classified with: http://purl.uniprot.org/keywords/574
classified with: GO_0042597
classified with: GO_0004067
classified with: GO_0050417
classified with: GO_0004359
classified with: GO_0006528
sequence: P10182-1

P10182-citation-8068664
type: Citation Statement
scope: PROTEIN SEQUENCE
scope: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS)

P10182-citation-619999
type: Citation Statement
scope: PROTEIN SEQUENCE OF 1-26

P10182-citation-9020792
type: Citation Statement
scope: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS)

P10182-citation-10684596
type: Citation Statement
scope: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 8-337 IN COMPLEXES WITH 6-DIAZO-5-OXO-L-NORLEUCINE AND 5-DIAZO-4-OXO-L-NORVALINE
scope: ACTIVE SITE
scope: SUBUNIT

1DJO
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.00
chain sequence mapping: PDB 1DJO tt8tt337

PDB 1DJO tt8tt337
chain: A/B=8-337

1DJP
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 1.90
chain sequence mapping: PDB 1DJP tt8tt337

PDB 1DJP tt8tt337
chain: A/B=8-337

3PGA
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.00
chain sequence mapping: PDB 3PGA tt8tt337

PDB 3PGA tt8tt337
chain: 1/2/3/4=8-337

4PGA
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 1.70
chain sequence mapping: PDB 4PGA tt1tt337

PDB 4PGA tt1tt337
chain: A/B=1-337

http://purl.uniprot.org/brenda/3.5.1.38
type: Resource
database: BRENDA
comment: 5085

cd00411
type: Resource
database: CDD
comment: L-asparaginase_like
signature sequence match: CDD cd00411 match 1

http://purl.uniprot.org/gene3d/3.40.50.1170
type: Resource
database: Gene3D
comment: L-asparaginase, N-terminal domain
signature sequence match: Gene3D 3.40.50.1170 match 1

IPR004550
type: Resource
database: InterPro
comment: AsnASE_II

IPR036152
type: Resource
database: InterPro
comment: Asp/glu_Ase-like_sf

IPR006034
type: Resource
database: InterPro
comment: Asparaginase/glutaminase-like

IPR020827
type: Resource
database: InterPro
comment: Asparaginase/glutaminase_AS1

IPR027475
type: Resource
database: InterPro
comment: Asparaginase/glutaminase_AS2

IPR040919
type: Resource
database: InterPro
comment: Asparaginase_C

IPR027473
type: Resource
database: InterPro
comment: L-asparaginase_C

IPR027474
type: Resource
database: InterPro
comment: L-asparaginase_N

IPR037152
type: Resource
database: InterPro
comment: L-asparaginase_N_sf

PTHR11707:SF28
type: Resource
database: PANTHER
comment: 60 KDA LYSOPHOSPHOLIPASE
signature sequence match: PANTHER PTHR11707:SF28 match 1

PTHR11707
type: Resource
database: PANTHER
comment: L-ASPARAGINASE
signature sequence match: PANTHER PTHR11707 match 1

PF00710
type: Resource
database: Pfam
comment: Asparaginase
signature sequence match: Pfam PF00710 match 1

PF17763
type: Resource
database: Pfam
comment: Asparaginase_C
signature sequence match: Pfam PF17763 match 1

PIRSF001220
type: Resource
database: PIRSF
comment: L-ASNase_gatD
signature sequence match: PIRSF PIRSF001220 match 1

PIRSF500176
type: Resource
database: PIRSF
comment: L_ASNase
signature sequence match: PIRSF PIRSF500176 match 1

PR00139
type: Resource
database: PRINTS
comment: ASNGLNASE

SM00870
type: Resource
database: SMART
comment: Asparaginase
signature sequence match: SMART SM00870 match 1

SSF53774
type: Resource
database: SUPFAM
comment: Glutaminase/Asparaginase
signature sequence match: SUPFAM SSF53774 match 1

TIGR00520
type: Resource
database: TIGRFAMs
comment: asnASE_II
signature sequence match: TIGRFAMs TIGR00520 match 1

PS00144
type: Resource
database: PROSITE
comment: ASN_GLN_ASE_1
signature sequence match: PROSITE PS00144 match 1

PS00917
type: Resource
database: PROSITE
comment: ASN_GLN_ASE_2
signature sequence match: PROSITE PS00917 match 1

PS51732
type: Resource
database: PROSITE
comment: ASN_GLN_ASE_3
signature sequence match: PROSITE PS51732 match 1

SIPC8BC812CE7E9D4B4
type: External Sequence
comment: AA sequence
citation: http://purl.uniprot.org/citations/619999

SIPD87756D7FEBCFCFD
type: Structured Name
full name: Glutaminase-asparaginase
ec name: 3.5.1.38

SIP753887D11BCB0194
type: Structured Name
full name: L-ASNase/L-GLNase

SIP2CCF2CFF0992EA89
type: Structured Name
full name: L-asparagine/L-glutamine amidohydrolase
short name: PGA

ansB
type: Gene

SIP53D6978A1B0ED1DE
type: Subunit Annotation
comment: Homotetramer.

SIPF41BA78C1170023A
type: Similarity Annotation
comment: Belongs to the asparaginase 1 family.

PRO_0000171089
type: Chain Annotation
comment: Glutaminase-asparaginase
mass: 36200
range: 22572081190810158tt1tt337

22572081190810158tt1
type: Position
type: ExactPosition
position: 1
reference: P10182-1

22572081190810158tt337
type: Position
type: ExactPosition
position: 337
reference: P10182-1

SIPB65FD4840891480D
type: Domain Extent Annotation
comment: Asparaginase/glutaminase
range: 22572081190810158tt10tt337

22572081190810158tt10
type: Position
type: ExactPosition
position: 10
reference: P10182-1

SIP01B9D40BB725A062
type: Active Site Annotation
comment: Acyl-ester intermediate
range: 22572081190810158tt20tt20

22572081190810158tt20
type: Position
type: ExactPosition
position: 20
reference: P10182-1

22572081190810158tt67
type: Position
type: ExactPosition
position: 67
reference: P10182-1

22572081190810158tt100
type: Position
type: ExactPosition
position: 100
reference: P10182-1

22572081190810158tt101
type: Position
type: ExactPosition
position: 101
reference: P10182-1

SIP16B8DB373625623F
type: Sequence Conflict Annotation
conflicting sequence: SIPC8BC812CE7E9D4B4
substitution: H
range: 22572081190810158tt7tt7

22572081190810158tt7
type: Position
type: ExactPosition
position: 7
reference: P10182-1

SIPE26164CA2452FD80
type: Sequence Conflict Annotation
conflicting sequence: SIPC8BC812CE7E9D4B4
substitution: R
range: 22572081190810158tt12tt12

22572081190810158tt12
type: Position
type: ExactPosition
position: 12
reference: P10182-1

22572081190810158tt11
type: Position
type: ExactPosition
position: 11
reference: P10182-1

22572081190810158tt19
type: Position
type: ExactPosition
position: 19
reference: P10182-1

22572081190810158tt22
type: Position
type: ExactPosition
position: 22
reference: P10182-1

22572081190810158tt26
type: Position
type: ExactPosition
position: 26
reference: P10182-1

22572081190810158tt29
type: Position
type: ExactPosition
position: 29
reference: P10182-1

22572081190810158tt31
type: Position
type: ExactPosition
position: 31
reference: P10182-1

22572081190810158tt37
type: Position
type: ExactPosition
position: 37
reference: P10182-1

22572081190810158tt41
type: Position
type: ExactPosition
position: 41
reference: P10182-1

22572081190810158tt46
type: Position
type: ExactPosition
position: 46
reference: P10182-1

22572081190810158tt51
type: Position
type: ExactPosition
position: 51
reference: P10182-1

22572081190810158tt54
type: Position
type: ExactPosition
position: 54
reference: P10182-1

22572081190810158tt56
type: Position
type: ExactPosition
position: 56
reference: P10182-1

22572081190810158tt65
type: Position
type: ExactPosition
position: 65
reference: P10182-1

22572081190810158tt69
type: Position
type: ExactPosition
position: 69
reference: P10182-1

22572081190810158tt72
type: Position
type: ExactPosition
position: 72
reference: P10182-1

22572081190810158tt87
type: Position
type: ExactPosition
position: 87
reference: P10182-1

22572081190810158tt92
type: Position
type: ExactPosition
position: 92
reference: P10182-1

22572081190810158tt97
type: Position
type: ExactPosition
position: 97
reference: P10182-1

22572081190810158tt102
type: Position
type: ExactPosition
position: 102
reference: P10182-1

22572081190810158tt103
type: Position
type: ExactPosition
position: 103
reference: P10182-1

22572081190810158tt113
type: Position
type: ExactPosition
position: 113
reference: P10182-1

22572081190810158tt120
type: Position
type: ExactPosition
position: 120
reference: P10182-1

22572081190810158tt123
type: Position
type: ExactPosition
position: 123
reference: P10182-1

22572081190810158tt136
type: Position
type: ExactPosition
position: 136
reference: P10182-1

22572081190810158tt147
type: Position
type: ExactPosition
position: 147
reference: P10182-1

22572081190810158tt150
type: Position
type: ExactPosition
position: 150
reference: P10182-1

22572081190810158tt152
type: Position
type: ExactPosition
position: 152
reference: P10182-1

22572081190810158tt157
type: Position
type: ExactPosition
position: 157
reference: P10182-1

22572081190810158tt159
type: Position
type: ExactPosition
position: 159
reference: P10182-1

22572081190810158tt164
type: Position
type: ExactPosition
position: 164
reference: P10182-1

22572081190810158tt167
type: Position
type: ExactPosition
position: 167
reference: P10182-1

22572081190810158tt168
type: Position
type: ExactPosition
position: 168
reference: P10182-1

22572081190810158tt170
type: Position
type: ExactPosition
position: 170
reference: P10182-1

22572081190810158tt171
type: Position
type: ExactPosition
position: 171
reference: P10182-1

22572081190810158tt173
type: Position
type: ExactPosition
position: 173
reference: P10182-1

22572081190810158tt175
type: Position
type: ExactPosition
position: 175
reference: P10182-1

22572081190810158tt178
type: Position
type: ExactPosition
position: 178
reference: P10182-1

22572081190810158tt182
type: Position
type: ExactPosition
position: 182
reference: P10182-1

22572081190810158tt184
type: Position
type: ExactPosition
position: 184
reference: P10182-1

22572081190810158tt189
type: Position
type: ExactPosition
position: 189
reference: P10182-1

22572081190810158tt193
type: Position
type: ExactPosition
position: 193
reference: P10182-1

22572081190810158tt196
type: Position
type: ExactPosition
position: 196
reference: P10182-1

22572081190810158tt199
type: Position
type: ExactPosition
position: 199
reference: P10182-1

22572081190810158tt201
type: Position
type: ExactPosition
position: 201
reference: P10182-1

22572081190810158tt203
type: Position
type: ExactPosition
position: 203
reference: P10182-1

22572081190810158tt208
type: Position
type: ExactPosition
position: 208
reference: P10182-1

22572081190810158tt210
type: Position
type: ExactPosition
position: 210
reference: P10182-1

22572081190810158tt215
type: Position
type: ExactPosition
position: 215
reference: P10182-1

22572081190810158tt217
type: Position
type: ExactPosition
position: 217
reference: P10182-1

22572081190810158tt224
type: Position
type: ExactPosition
position: 224
reference: P10182-1

22572081190810158tt228
type: Position
type: ExactPosition
position: 228
reference: P10182-1

22572081190810158tt236
type: Position
type: ExactPosition
position: 236
reference: P10182-1

22572081190810158tt243
type: Position
type: ExactPosition
position: 243
reference: P10182-1

22572081190810158tt247
type: Position
type: ExactPosition
position: 247
reference: P10182-1

22572081190810158tt254
type: Position
type: ExactPosition
position: 254
reference: P10182-1

22572081190810158tt255
type: Position
type: ExactPosition
position: 255
reference: P10182-1

22572081190810158tt257
type: Position
type: ExactPosition
position: 257
reference: P10182-1

22572081190810158tt261
type: Position
type: ExactPosition
position: 261
reference: P10182-1

22572081190810158tt263
type: Position
type: ExactPosition
position: 263
reference: P10182-1

22572081190810158tt264
type: Position
type: ExactPosition
position: 264
reference: P10182-1

22572081190810158tt272
type: Position
type: ExactPosition
position: 272
reference: P10182-1

22572081190810158tt276
type: Position
type: ExactPosition
position: 276
reference: P10182-1

22572081190810158tt282
type: Position
type: ExactPosition
position: 282
reference: P10182-1

22572081190810158tt291
type: Position
type: ExactPosition
position: 291
reference: P10182-1

22572081190810158tt293
type: Position
type: ExactPosition
position: 293
reference: P10182-1

22572081190810158tt297
type: Position
type: ExactPosition
position: 297
reference: P10182-1

22572081190810158tt300
type: Position
type: ExactPosition
position: 300
reference: P10182-1

22572081190810158tt310
type: Position
type: ExactPosition
position: 310
reference: P10182-1

22572081190810158tt320
type: Position
type: ExactPosition
position: 320
reference: P10182-1

22572081190810158tt321
type: Position
type: ExactPosition
position: 321
reference: P10182-1

22572081190810158tt323
type: Position
type: ExactPosition
position: 323
reference: P10182-1

22572081190810158tt327
type: Position
type: ExactPosition
position: 327
reference: P10182-1

22572081190810158tt336
type: Position
type: ExactPosition
position: 336
reference: P10182-1

substrate

P10182-1
type: Simple Sequence
modified: 1995-11-01
version: 2
mass: 36200
value: KEVENQQKLANVVILATGGTIAGAGASAANSATYQAAKVGVDKLIAGVPELADLANVRGEQVMQIASESITNDDLLKLGKRVAELADSNDVDGIVITHGTDTLEETAYFLDLTLNTDKPIVVVGSMRPGTAMSADGMLNLYNAVAVASNKDSRGKGVLVTMNDEIQSGRDVSKSINIKTEAFKSAWGPLGMVVEGKSYWFRLPAKRHTVNSEFDIKQISSLPQVDIAYSYGNVTDTAYKALAQNGAKALIHAGTGNGSVSSRLTPALQTLRKTGTQIIRSSHVNQGGFVLRNAEQPDDKNDWVVAHDLNPEKARILVELAMVKTQDSKELQRIFWEY

http://purl.uniprot.org/uniprot/P10182#attribution-609619A7DF3E53CE5E48EDEF8B8F9DD1
manual: true
evidence: ECO_0000250

http://purl.uniprot.org/uniprot/P10182#attribution-DEE029FAFEC36C9A3DB22B3BAA55AD10
manual: true
evidence: ECO_0000255
source: PRU01068

http://purl.uniprot.org/uniprot/P10182#attribution-9F7733B5CDF7129FA0002D840D9B58EF
manual: true
evidence: ECO_0000255
source: PRU10099

http://purl.uniprot.org/uniprot/P10182#attribution-95ED5DAF9A98D5C5CE5584DB38EEB18E
manual: true
evidence: ECO_0000255
source: PRU10100

http://purl.uniprot.org/uniprot/P10182#attribution-E4E7F5AAED3EB276BBF423890489D827
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/10684596

http://purl.uniprot.org/uniprot/P10182#attribution-080ED3982FEC43F7C43A0689717F3D20
manual: true
evidence: ECO_0000305

P72156
type: Protein
reviewed: true
created: 1998-07-15
modified: 2023-05-03
version: 106
mnemonic: ATZA_PSESD
replaces: Q79BR5
citation: http://purl.uniprot.org/citations/7574646
citation: http://purl.uniprot.org/citations/8759853
citation: SIP2988BEE243D011B9
citation: http://purl.uniprot.org/citations/11274097
citation: http://purl.uniprot.org/citations/12450410
citation: http://purl.uniprot.org/citations/22768133
citation: http://purl.uniprot.org/citations/25760618
recommended name: SIP5197A22A807A764D
enzyme: http://purl.uniprot.org/enzyme/3.8.1.8
organism: http://purl.uniprot.org/taxonomy/47660
encoded in: pADP-1
annotation: SIP011F74BF29D29BDF
annotation: SIP170F25BF84B96152
annotation: SIP8EC537CBF1C1A247
annotation: SIPF5C64F5FA4A155B2
annotation: SIPC4285D143205A74E
annotation: SIP3001E181FAD62FA7
annotation: SIP3CC8B0E6F054D9FF
annotation: SIP328E96E0911B4827
annotation: SIP787768F4A69E908D
annotation: SIP0EA3317268722227
annotation: PRO_0000122293
annotation: SIPC87CEB67ED9AA50B
annotation: SIPFFA9634422AB2B40
annotation: SIP3AF0CE0F327F0CC1
annotation: SIPA69E51CDA823F255
annotation: SIPA03C8F7710B05B5D
annotation: SIP93FFBA6D17840CD6
annotation: SIPD63658E27057D07C
annotation: SIP7A2EE24D16872B1A
annotation: SIPB6B0A4F1993DDA12
annotation: SIP251CCA403155BCA5
annotation: SIP91993A542CD32AEA
annotation: SIP691D21B417C39A1B
annotation: SIPBEFF4BC915D45FDE
annotation: SIP438C0D067215EC9E
annotation: SIP7C86E3A3011E2265
annotation: SIPCF476878FED4AB6F
annotation: SIPCF2770193B46FEFF
annotation: SIPFC4AA25193F80C03
annotation: SIP09001B5B2A666B3B
annotation: SIPF9DD091DF5BAE092
annotation: SIP56E5586171935816
annotation: SIPCEF959EA7B67A364
annotation: SIPFAD1F83961D96B6C
annotation: SIP53F4CC2AF6DE5BF9
annotation: SIP45A9C10BFB457986
annotation: SIPE9E77E5552A4DF2F
annotation: SIPA3422A619B3EACA7
annotation: SIP3CA400A44399BA7B
annotation: SIPE3E64B2B1EB073EF
annotation: SIP0131D6165DD5E345
annotation: SIP04755FB024F69785
annotation: SIP1BF9AD6B0FF6D2C1
annotation: SIP625749E90E2A9750
annotation: SIPC0083BF4CA441199
annotation: SIPE8EE5C13390BE567
annotation: SIP40A06661D37B0F37
annotation: SIP380D5937D9B327AC
annotation: SIPB9ECFE0C16580738
annotation: SIPD0CC4F56CE34FA7D
annotation: SIPD8206BBBCCCF36CF
annotation: SIPA62487BE5E0B569D
annotation: SIPCB003DC5ECB8F53E
annotation: SIP190CA59A9E521163
annotation: SIP59A30014AE1C8B49
annotation: SIPC367EE21D68371A2
annotation: SIP8C25A315BC40D18F
annotation: SIP12CBF7EF3053C742
annotation: SIPC44EEE88C09C16FF
annotation: SIPC62BC74EFA3E0D9A
annotation: SIPB3D401CDD7B68C32
annotation: SIPE461F58D7FE54BEF

P72156-citation-7574646
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
scope: PATHWAY

P72156-citation-8759853
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
scope: PROTEIN SEQUENCE OF 1-10
scope: FUNCTION
scope: CATALYTIC ACTIVITY
scope: BIOPHYSICOCHEMICAL PROPERTIES

SIP2988BEE243D011B9
type: Submission Citation
author: de Souza M.L.
author: Sadowsky M.J.
author: Wackett L.P.
date: 1998-10
submitted to: EMBL/GenBank/DDBJ

P72156-citation-SIP2988BEE243D011B9
type: Citation Statement
scope: SEQUENCE REVISION TO 335 AND 400-406

P72156-citation-11274097
type: Citation Statement
scope: ACTIVITY REGULATION
scope: SIMILARITY WITH TRIA

NRRL B-12227
type: Strain

P72156-citation-12450410
type: Citation Statement
scope: CHARACTERIZATION OF METAL REQUIREMENT
scope: COFACTOR

P72156-citation-22768133
type: Citation Statement
scope: FUNCTION
scope: CATALYTIC ACTIVITY
scope: MUTAGENESIS OF PHE-84; ASN-328 AND SER-331

P72156-citation-25760618
type: Citation Statement
scope: X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS) IN COMPLEX WITH IRON
scope: COFACTOR
scope: SUBUNIT

4V1X
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.20
chain sequence mapping: PDB 4V1X tt1tt474

PDB 4V1X tt1tt474
chain: A/B/C/D/E/F=1-474

4V1Y
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.80
chain sequence mapping: PDB 4V1Y tt1tt474

PDB 4V1Y tt1tt474
chain: A/B/C/D/E/F/G/H/I/J/K/L=1-474

http://purl.uniprot.org/brenda/3.8.1.8
type: Resource
database: BRENDA
comment: 5085

cd01298
type: Resource
database: CDD
comment: ATZ_TRZ_like
signature sequence match: CDD cd01298 match 1

http://purl.uniprot.org/gene3d/3.20.20.140
type: Resource
database: Gene3D
comment: Metal-dependent hydrolases
signature sequence match: Gene3D 3.20.20.140 match 1

http://purl.uniprot.org/gene3d/2.30.40.10
type: Resource
database: Gene3D
comment: Urease, subunit C, domain 1
signature sequence match: Gene3D 2.30.40.10 match 1

IPR006680
type: Resource
database: InterPro
comment: Amidohydro-rel

IPR011059
type: Resource
database: InterPro
comment: Metal-dep_hydrolase_composite

IPR032466
type: Resource
database: InterPro
comment: Metal_Hydrolase

PTHR43794
type: Resource
database: PANTHER
comment: AMINOHYDROLASE SSNA-RELATED
signature sequence match: PANTHER PTHR43794 match 1

PTHR43794:SF5
type: Resource
database: PANTHER
comment: CHLOROHYDROLASE FAMILY PROTEIN
signature sequence match: PANTHER PTHR43794:SF5 match 1

PF01979
type: Resource
database: Pfam
comment: Amidohydro_1
signature sequence match: Pfam PF01979 match 1

SSF51338
type: Resource
database: SUPFAM
comment: Composite domain of metallo-dependent hydrolases
signature sequence match: SUPFAM SSF51338 match 1
signature sequence match: SUPFAM SSF51338 match 2

SSF51556
type: Resource
database: SUPFAM
comment: Metallo-dependent hydrolases
signature sequence match: SUPFAM SSF51556 match 1

SIP5197A22A807A764D
type: Structured Name
full name: Atrazine chlorohydrolase
ec name: 3.8.1.8

pADP-1
type: Plasmid

atzA
type: Gene

SIP011F74BF29D29BDF
type: Function Annotation
comment: Hydrolytically dechlorinates atrazine to hydroxyatrazine. Dechlorinates also simazine, and desethylatrazine but is not active with terbutylazine or desethyldesisopropylatrazine (PubMed:8759853). Has no deaminase activity with melamine (PubMed:8759853, PubMed:22768133).

SIP8EC537CBF1C1A247
type: Cofactor Annotation
comment: Binds 1 Fe(2+) ion per subunit (PubMed:12450410, PubMed:25760618). In vitro can also use Co(2+) or Mn(2+) (PubMed:12450410).
cofactor: CHEBI_29033

SIPF5C64F5FA4A155B2
type: Activity Regulation Annotation
comment: Atrazine dechlorination rates are reduced by aminoatrazine but dechlorination is not inhibited by either melanine or CAAT (2-chloro-4,6-diamino-s-triazine).

SIPC4285D143205A74E
type: Kinetics Annotation
comment: kcat is 11 sec(-1) with atrazine as substrate.
measured affinity: 149 uM for atrazine
measured activity: 2.6 umol/min/mg enzyme

SIP3CC8B0E6F054D9FF
type: Subunit Annotation
comment: Homohexamer; trimer of dimers.

SIP787768F4A69E908D
type: Annotation
comment: AtzA and melamine deaminase (TriA) from Acidovorax citrulli strain NRRL B-12227 are 98% identical but catalyze distinct biochemical reactions. Enzymes differ by only 9 amino acids. These 9 amino acid differences between TriA and AtzA represent a short evolutionary pathway connecting enzymes catalyzing physiologically relevant deamination and dehalogenation reactions, respectively (PubMed:11274097). Potential evolutionary trajectories between AtzA and TriA have been constructed, and catalytic activities of the intermediates along those trajectories have been characterized (PubMed:22768133).

SIP0EA3317268722227
type: Similarity Annotation
comment: Belongs to the metallo-dependent hydrolases superfamily. ATZ/TRZ family.

PRO_0000122293
type: Chain Annotation
comment: Atrazine chlorohydrolase
mass: 52486
range: 22578686850315822tt1tt474

22578686850315822tt1
type: Position
type: ExactPosition
position: 1
reference: P72156-1

22578686850315822tt474
type: Position
type: ExactPosition
position: 474
reference: P72156-1

22578686850315822tt66
type: Position
type: ExactPosition
position: 66
reference: P72156-1

22578686850315822tt68
type: Position
type: ExactPosition
position: 68
reference: P72156-1

22578686850315822tt276
type: Position
type: ExactPosition
position: 276
reference: P72156-1

22578686850315822tt327
type: Position
type: ExactPosition
position: 327
reference: P72156-1

SIPA03C8F7710B05B5D
type: Site Annotation
comment: Major determinant of atrazine specificity
range: 22578686850315822tt84tt84

22578686850315822tt84
type: Position
type: ExactPosition
position: 84
reference: P72156-1

SIP93FFBA6D17840CD6
type: Site Annotation
comment: Major determinant of atrazine specificity
range: 22578686850315822tt328tt328

22578686850315822tt328
type: Position
type: ExactPosition
position: 328
reference: P72156-1

SIPD63658E27057D07C
type: Site Annotation
comment: Major determinant of atrazine specificity
range: 22578686850315822tt331tt331

22578686850315822tt331
type: Position
type: ExactPosition
position: 331
reference: P72156-1

SIP7A2EE24D16872B1A
type: Mutagenesis Annotation
comment: 6.1-fold decrease in catalytic efficiency with atrazine. Does not confer melamine deaminase activity. 28.5-fold decrease in catalytic efficiency with atrazine and strong increase in melanine deaminase activity; when associated with D-328 and C-331.
substitution: L
range: 22578686850315822tt84tt84

SIPB6B0A4F1993DDA12
type: Mutagenesis Annotation
comment: 24.3-fold decrease in catalytic efficiency with atrazine. Does not confer melamine deaminase activity. 28.5-fold decrease in catalytic efficiency with atrazine and strong increase in melanine deaminase activity; when associated with L-84 and C-331.
substitution: D
range: 22578686850315822tt328tt328

SIP251CCA403155BCA5
type: Mutagenesis Annotation
comment: 4.6-fold decrease in catalytic efficiency with atrazine. Confers weak melamine deaminase activity. 28.5-fold decrease in catalytic efficiency with atrazine and strong increase in melanine deaminase activity; when associated with L-84 and D-328.
substitution: C
range: 22578686850315822tt331tt331

22578686850315822tt3
type: Position
type: ExactPosition
position: 3
reference: P72156-1

22578686850315822tt12
type: Position
type: ExactPosition
position: 12
reference: P72156-1

22578686850315822tt20
type: Position
type: ExactPosition
position: 20
reference: P72156-1

22578686850315822tt29
type: Position
type: ExactPosition
position: 29
reference: P72156-1

22578686850315822tt32
type: Position
type: ExactPosition
position: 32
reference: P72156-1

22578686850315822tt38
type: Position
type: ExactPosition
position: 38
reference: P72156-1

22578686850315822tt39
type: Position
type: ExactPosition
position: 39
reference: P72156-1

22578686850315822tt41
type: Position
type: ExactPosition
position: 41
reference: P72156-1

22578686850315822tt48
type: Position
type: ExactPosition
position: 48
reference: P72156-1

22578686850315822tt52
type: Position
type: ExactPosition
position: 52
reference: P72156-1

22578686850315822tt57
type: Position
type: ExactPosition
position: 57
reference: P72156-1

22578686850315822tt60
type: Position
type: ExactPosition
position: 60
reference: P72156-1

22578686850315822tt62
type: Position
type: ExactPosition
position: 62
reference: P72156-1

22578686850315822tt70
type: Position
type: ExactPosition
position: 70
reference: P72156-1

22578686850315822tt74
type: Position
type: ExactPosition
position: 74
reference: P72156-1

22578686850315822tt76
type: Position
type: ExactPosition
position: 76
reference: P72156-1

22578686850315822tt81
type: Position
type: ExactPosition
position: 81
reference: P72156-1

22578686850315822tt90
type: Position
type: ExactPosition
position: 90
reference: P72156-1

22578686850315822tt92
type: Position
type: ExactPosition
position: 92
reference: P72156-1

22578686850315822tt98
type: Position
type: ExactPosition
position: 98
reference: P72156-1

22578686850315822tt101
type: Position
type: ExactPosition
position: 101
reference: P72156-1

22578686850315822tt117
type: Position
type: ExactPosition
position: 117
reference: P72156-1

22578686850315822tt120
type: Position
type: ExactPosition
position: 120
reference: P72156-1

22578686850315822tt127
type: Position
type: ExactPosition
position: 127
reference: P72156-1

22578686850315822tt128
type: Position
type: ExactPosition
position: 128
reference: P72156-1

22578686850315822tt131
type: Position
type: ExactPosition
position: 131
reference: P72156-1

22578686850315822tt135
type: Position
type: ExactPosition
position: 135
reference: P72156-1

22578686850315822tt146
type: Position
type: ExactPosition
position: 146
reference: P72156-1

22578686850315822tt149
type: Position
type: ExactPosition
position: 149
reference: P72156-1

22578686850315822tt156
type: Position
type: ExactPosition
position: 156
reference: P72156-1

22578686850315822tt162
type: Position
type: ExactPosition
position: 162
reference: P72156-1

22578686850315822tt164
type: Position
type: ExactPosition
position: 164
reference: P72156-1

22578686850315822tt165
type: Position
type: ExactPosition
position: 165
reference: P72156-1

22578686850315822tt173
type: Position
type: ExactPosition
position: 173
reference: P72156-1

22578686850315822tt187
type: Position
type: ExactPosition
position: 187
reference: P72156-1

22578686850315822tt201
type: Position
type: ExactPosition
position: 201
reference: P72156-1

22578686850315822tt202
type: Position
type: ExactPosition
position: 202
reference: P72156-1

22578686850315822tt208
type: Position
type: ExactPosition
position: 208
reference: P72156-1

22578686850315822tt209
type: Position
type: ExactPosition
position: 209
reference: P72156-1

22578686850315822tt215
type: Position
type: ExactPosition
position: 215
reference: P72156-1

22578686850315822tt218
type: Position
type: ExactPosition
position: 218
reference: P72156-1

22578686850315822tt220
type: Position
type: ExactPosition
position: 220
reference: P72156-1

22578686850315822tt223
type: Position
type: ExactPosition
position: 223
reference: P72156-1

22578686850315822tt235
type: Position
type: ExactPosition
position: 235
reference: P72156-1

22578686850315822tt240
type: Position
type: ExactPosition
position: 240
reference: P72156-1

22578686850315822tt245
type: Position
type: ExactPosition
position: 245
reference: P72156-1

22578686850315822tt248
type: Position
type: ExactPosition
position: 248
reference: P72156-1

22578686850315822tt250
type: Position
type: ExactPosition
position: 250
reference: P72156-1

22578686850315822tt258
type: Position
type: ExactPosition
position: 258
reference: P72156-1

22578686850315822tt264
type: Position
type: ExactPosition
position: 264
reference: P72156-1

22578686850315822tt272
type: Position
type: ExactPosition
position: 272
reference: P72156-1

22578686850315822tt282
type: Position
type: ExactPosition
position: 282
reference: P72156-1

22578686850315822tt290
type: Position
type: ExactPosition
position: 290
reference: P72156-1

22578686850315822tt294
type: Position
type: ExactPosition
position: 294
reference: P72156-1

22578686850315822tt297
type: Position
type: ExactPosition
position: 297
reference: P72156-1

22578686850315822tt299
type: Position
type: ExactPosition
position: 299
reference: P72156-1

22578686850315822tt304
type: Position
type: ExactPosition
position: 304
reference: P72156-1

22578686850315822tt312
type: Position
type: ExactPosition
position: 312
reference: P72156-1

22578686850315822tt317
type: Position
type: ExactPosition
position: 317
reference: P72156-1

22578686850315822tt322
type: Position
type: ExactPosition
position: 322
reference: P72156-1

22578686850315822tt324
type: Position
type: ExactPosition
position: 324
reference: P72156-1

22578686850315822tt337
type: Position
type: ExactPosition
position: 337
reference: P72156-1

22578686850315822tt352
type: Position
type: ExactPosition
position: 352
reference: P72156-1

22578686850315822tt360
type: Position
type: ExactPosition
position: 360
reference: P72156-1

22578686850315822tt367
type: Position
type: ExactPosition
position: 367
reference: P72156-1

22578686850315822tt369
type: Position
type: ExactPosition
position: 369
reference: P72156-1

22578686850315822tt374
type: Position
type: ExactPosition
position: 374
reference: P72156-1

22578686850315822tt378
type: Position
type: ExactPosition
position: 378
reference: P72156-1

22578686850315822tt380
type: Position
type: ExactPosition
position: 380
reference: P72156-1

22578686850315822tt381
type: Position
type: ExactPosition
position: 381
reference: P72156-1

22578686850315822tt383
type: Position
type: ExactPosition
position: 383
reference: P72156-1

22578686850315822tt392
type: Position
type: ExactPosition
position: 392
reference: P72156-1

22578686850315822tt395
type: Position
type: ExactPosition
position: 395
reference: P72156-1

22578686850315822tt408
type: Position
type: ExactPosition
position: 408
reference: P72156-1

22578686850315822tt414
type: Position
type: ExactPosition
position: 414
reference: P72156-1

22578686850315822tt421
type: Position
type: ExactPosition
position: 421
reference: P72156-1

22578686850315822tt426
type: Position
type: ExactPosition
position: 426
reference: P72156-1

22578686850315822tt429
type: Position
type: ExactPosition
position: 429
reference: P72156-1

22578686850315822tt433
type: Position
type: ExactPosition
position: 433
reference: P72156-1

22578686850315822tt442
type: Position
type: ExactPosition
position: 442
reference: P72156-1

22578686850315822tt462
type: Position
type: ExactPosition
position: 462
reference: P72156-1

P72156-1
type: Simple Sequence
modified: 2000-05-30
version: 2
mass: 52486
value: MQTLSIQHGTLVTMDQYRRVLGDSWVHVQDGRIVALGVHAESVPPPADRVIDARGKVVLPGFINAHTHVNQILLRGGPSHGRQFYDWLFNVVYPGQKAMRPEDVAVAVRLYCAEAVRSGITTINENADSAIYPGNIEAAMAVYGEVGVRVVYARMFFDRMDGRIQGYVDALKARSPQVELCSIMEETAVAKDRITALSDQYHGTAGGRISVWPAPATTTAVTVEGMRWAQAFARDRAVMWTLHMAESDHDERIHGMSPAEYMECYGLLDERLQVAHCVYFDRKDVRLLHRHNVKVASQVVSNAYLGSGVAPVPEMVERGMAVGIGTDNGNSNDSVNMIGDMKFMAHIHRAVHRDADVLTPEKILEMATIDGARSLGMDHEIGSIETGKRADLILLDLRHPQTTPHHHLAATIVFQAYGNEVDTVLIDGNVVMENRRLSFLPPERELAFLEEAQSRATAILQRANMVANPAWRSL

http://purl.uniprot.org/uniprot/P72156#attribution-2DD11508BC89F7C94659EE490B228827
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/11274097

http://purl.uniprot.org/uniprot/P72156#attribution-99883FFAEA67D56A4D223A9E959D660E
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/12450410

http://purl.uniprot.org/uniprot/P72156#attribution-9AA43DBE9A548EA8FF70C5AF95E60282
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/22768133

http://purl.uniprot.org/uniprot/P72156#attribution-A1143649C8C8F0E6A7F956B73BAB790C
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/25760618

http://purl.uniprot.org/uniprot/P72156#attribution-3AA50F721E0A92F15F0F36A843ED9886
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/8759853

http://purl.uniprot.org/uniprot/P72156#attribution-8D5F6D47D3D36C76CAA34B29ADFD0065
manual: true
evidence: ECO_0000303
source: http://purl.uniprot.org/citations/7574646

http://purl.uniprot.org/uniprot/P72156#attribution-4623FFB1D61F1D4DB3A707B572C79D12
manual: true
evidence: ECO_0000303
source: http://purl.uniprot.org/citations/8759853

http://purl.uniprot.org/uniprot/P72156#attribution-FF980F6EDE09BD7F353A39E3A346AFC0
manual: true
evidence: ECO_0000305

http://purl.uniprot.org/uniprot/P72156#attribution-535D2389AF34D4F0D975020A494005A2
manual: true
evidence: ECO_0000305
source: http://purl.uniprot.org/citations/11274097

http://purl.uniprot.org/uniprot/P72156#attribution-1D593046295570CD210C2C18BB232D0F
manual: true
evidence: ECO_0000305
source: http://purl.uniprot.org/citations/22768133

http://purl.uniprot.org/uniprot/P72156#attribution-30F2C670A916A2ACBB2CE24DC6C5619D
manual: true
evidence: ECO_0000305
source: http://purl.uniprot.org/citations/7574646

http://purl.uniprot.org/uniprot/P72156#attribution-DC2713F54B4C4B8561962C8259E585F8
manual: true
evidence: ECO_0007744
source: 4V1X

http://purl.uniprot.org/uniprot/P72156#attribution-27BF8D705F624D62316475D38C403108
manual: true
evidence: ECO_0007744
source: 4V1Y

P95442
type: Protein
reviewed: true
created: 1998-07-15
modified: 2023-05-03
version: 97
mnemonic: ATZB_PSESD
citation: http://purl.uniprot.org/citations/9055410
citation: http://purl.uniprot.org/citations/17660279
recommended name: SIP32717926D7BEB120
enzyme: http://purl.uniprot.org/enzyme/3.5.4.43
organism: http://purl.uniprot.org/taxonomy/47660
encoded in: pADP-1
annotation: SIP5E83BD9BA8CC7290
annotation: SIP6AD1C425CD650303
annotation: SIPBC87BBC41E211645
annotation: SIP4A305D171AD57429
annotation: SIP6DE5E760DC1278F0
annotation: SIP688960AADBC22FC0
annotation: SIP12AF2692DF221E1F
annotation: SIPF46CF90332A215E9
annotation: SIPCC1295FFF4FE767B
annotation: PRO_0000122295
annotation: SIP0D20BC9780B36B3E
annotation: SIP6404388031C51565
annotation: SIPBDC635D605048522
annotation: SIPD634039B0AD5FF3F
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/963
classified with: http://purl.uniprot.org/keywords/378
classified with: http://purl.uniprot.org/keywords/479
classified with: http://purl.uniprot.org/keywords/614
classified with: http://purl.uniprot.org/keywords/862
classified with: GO_0005737
classified with: GO_0018763
classified with: GO_0046872
classified with: GO_0019381
sequence: P95442-1

P95442-citation-9055410
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

P95442-citation-17660279
type: Citation Statement
scope: FUNCTION
scope: CATALYTIC ACTIVITY
scope: SUBSTRATE SPECIFICITY
scope: BIOPHYSICOCHEMICAL PROPERTIES
scope: COFACTOR
scope: SUBUNIT

PTHR43794:SF11
type: Resource
database: PANTHER
comment: AMIDOHYDRO-REL DOMAIN-CONTAINING PROTEIN
signature sequence match: PANTHER PTHR43794:SF11 match 1

SIP32717926D7BEB120
type: Structured Name
full name: Hydroxydechloroatrazine ethylaminohydrolase
short name: Hydroxyatrazine hydrolase
ec name: 3.5.4.43

pADP-1
type: Plasmid

atzB
type: Gene

SIP5E83BD9BA8CC7290
type: Function Annotation
comment: Catalyzes the deamination reaction of hydroxyatrazine to N-isopropylammelide (dihydroxy-isopropyl-atrazine). The enzyme is also capable of catalyzing some dechlorinating reactions.

SIPBC87BBC41E211645
type: Cofactor Annotation
comment: Binds 1 zinc ion per subunit.
cofactor: CHEBI_29105

SIP4A305D171AD57429
type: Kinetics Annotation
measured affinity: 20 uM for hydroxyatrazine
measured affinity: 40 uM for 6-hydroxy-4-(N-isopropylamino)-2-(N-methylamino)-1,3,5-triazine
measured affinity: 29 uM for 2,4-diisopropylamino-6-hydroxy-1,3,5-triazine
measured affinity: 120 uM for 2-chloro-4-hydroxy-6-(N-isopropylamino)-1,3,5-triazine
measured affinity: 230 uM for 2-chloro-4-(N-ethylamino)-6-hydroxy-1,3,5-triazine

SIP6DE5E760DC1278F0
type: PH Dependence Annotation
comment: Optimum pH is 6.5-7.5.

SIP12AF2692DF221E1F
type: Subunit Annotation
comment: Homodimer.

SIPCC1295FFF4FE767B
type: Similarity Annotation
comment: Belongs to the metallo-dependent hydrolases superfamily. ATZ/TRZ family.

PRO_0000122295
type: Chain Annotation
comment: Hydroxydechloroatrazine ethylaminohydrolase
mass: 52113
range: 22580898808738350tt1tt481

22580898808738350tt1
type: Position
type: ExactPosition
position: 1
reference: P95442-1

22580898808738350tt481
type: Position
type: ExactPosition
position: 481
reference: P95442-1

22580898808738350tt74
type: Position
type: ExactPosition
position: 74
reference: P95442-1

22580898808738350tt76
type: Position
type: ExactPosition
position: 76
reference: P95442-1

22580898808738350tt245
type: Position
type: ExactPosition
position: 245
reference: P95442-1

22580898808738350tt331
type: Position
type: ExactPosition
position: 331
reference: P95442-1

P95442-1
type: Simple Sequence
modified: 1997-05-01
version: 1
mass: 52113
value: MTTTLYTGFHQLVTGDVAGTVLNGVDILVRDGEIIGLGPDLPRTLAPIGVGQEQGVEVVNCRGLTAYPGLINTHHHFFQAFVRNLAPLDWTQLDVLAWLRKIYPVFALVDEDCIYHSTVVSMAELIKHGCTTAFDHQYNYSRRGGPFLVDRQFDAANLLGLRFHAGRGCITLPMAEGSTIPDAMRESTDTFLADCERLVSRFHDPRPFAMQRVVVAPSSPVIAYPETFVESARLARHLGVSLHTHLGEGETPAMVARFGERSLDWCENRGFVGPDVWLAHGWEFTAADIARLAATGTGVAHCPAPVFLVGAEVTDIPAMAAAGVRVGFGVDGHASNDSSNLAECIRLAYLLQCLKASERQHPVPAPYDFLRMATQGGADCLNRPDLGALAVGRAADFFAVDLNRIEYIGANHDPRSLPAKVGFSGPVDMTVINGKVVWRNGEFPGLDEMELARAADGVFRRVIYGDPLVAALRRGTGVTPC

http://purl.uniprot.org/uniprot/P95442#attribution-609619A7DF3E53CE5E48EDEF8B8F9DD1
manual: true
evidence: ECO_0000250

http://purl.uniprot.org/uniprot/P95442#attribution-270A34BEB6EAE28CFA0D63E9FDDB9643
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/17660279

http://purl.uniprot.org/uniprot/P95442#attribution-080ED3982FEC43F7C43A0689717F3D20
manual: true
evidence: ECO_0000305

O52063
type: Protein
reviewed: true
created: 1998-07-15
modified: 2023-05-03
version: 99
mnemonic: ATZC_PSESD
citation: http://purl.uniprot.org/citations/9422605
citation: http://purl.uniprot.org/citations/12218024
citation: SIP79F1302C81D2BF93
citation: http://purl.uniprot.org/citations/26390431
recommended name: SIPCDC1461A7CD2B744
enzyme: http://purl.uniprot.org/enzyme/3.5.4.42
organism: http://purl.uniprot.org/taxonomy/47660
encoded in: pADP-1
annotation: SIP3A175567F31F5C15
annotation: SIP8CE040410D6E10E4
annotation: SIP057E5B0A883BC275
annotation: SIP87E446E52DBFACD7
annotation: SIP42ADCBCCF64BD0A0
annotation: SIP668B5D36137DADF2
annotation: SIP465880E49E8A57A8
annotation: SIPBFC15598407CD4EE
annotation: SIP842411AFB3CA9D0E
annotation: SIP32A94ED4DD3C5040
annotation: PRO_0000182707
annotation: SIPAE354FD4DF2651B6
annotation: SIPE8EFC8FB8D5EB793
annotation: SIP5CEBF65A535D1F22
annotation: SIPA83EFC197103FE81
annotation: SIP01218CD947DC3708
annotation: SIP220ECFCEB7518DD8
annotation: SIPBE63C3BD83D7ADD8
annotation: SIP6A967D49D0D1FECF
annotation: SIP3534FB9537CF31EC
annotation: SIP3CBEE7630C64E214
annotation: SIPB1D7F9F2A1886655
annotation: SIP46CE8380908D9B7D
annotation: SIP8A94E6FB2478DF76
annotation: SIPEA305495CE3F3551
annotation: SIPECD897AC7EE1E1CF
annotation: SIPF494634A375A1D88
annotation: SIP5E17DDE7CDDE32BC
annotation: SIP14B1A07EF0C00696
annotation: SIPAD989EA9F0907AEE
annotation: SIP426AC370A2A56BA1
annotation: SIP4D63BC85ECAD4EB3
annotation: SIP59D69066416C7C01
annotation: SIP255AD8627D2B2B6D
annotation: SIP8589406407A13E22
annotation: SIP5AA050283018EA8E
annotation: SIP8F9F2B57C9C48104
annotation: SIP4BC791952E836324
annotation: SIPC5D11A972DF12AD1
annotation: SIPD025850DF127C33D
annotation: SIP666DF5DD17BF5E80
annotation: SIP4E60C2BFF9966B57
annotation: SIPCE8861F80C4D92C0
annotation: SIPFF8A18FCF72A545C
annotation: SIP361D5E621E6A55FE
annotation: SIP061838E9623DA1A1
annotation: SIP49F64CD5A2352102
annotation: SIP190598563B079A3F
annotation: SIP17BCC801DB42500B
annotation: SIP7D0C32DC659CC7A8
annotation: SIP60043E047BF5DE3E
annotation: SIPD9A94630D8D5A7C9
annotation: SIP6F802B6FCE72D124
annotation: SIP23C41408631E5DAA
annotation: SIP3ADAB9B122B91513
annotation: SIP90BA18FB5862FF7A
annotation: SIPA5A4B03A3CF4F130
annotation: SIP0F8CF7F4764C8996
annotation: SIP6449625C0C460AF3
annotation: SIPE3FA42F9296A9C00
annotation: SIP4134813C48BA8CBC
annotation: SIP0389E8FCA927A0A9
annotation: SIP0E9DCC4DAEAF9628
annotation: SIP201EF76C9C1452FC
annotation: SIP46BE3368D08616DD
annotation: SIPE3000732C6EE71A2

O52063-citation-9422605
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
scope: FUNCTION
scope: CATALYTIC ACTIVITY

O52063-citation-12218024
type: Citation Statement
scope: FUNCTION
scope: COFACTOR
scope: BIOPHYSICOCHEMICAL PROPERTIES
scope: SUBUNIT
scope: ACTIVITY REGULATION
scope: EPR SPECTROSCOPY

ADP
type: Strain

SIP79F1302C81D2BF93
type: Submission Citation
title: Crystal structure of N-isopropylammelide isopropylaminohydrolase AtzC from Pseudomonas sp. strain ADP complexed with Zn.
author: Fedorov A.A.
author: Fedorov E.V.
author: Seffernick J.
author: Wackett L.P.
author: Burley S.K.
author: Almo S.C.
date: 2007-08
submitted to: PDB

O52063-citation-SIP79F1302C81D2BF93
type: Citation Statement
scope: X-RAY CRYSTALLOGRAPHY (2.24 ANGSTROMS) IN COMPLEX WITH ZINC
scope: COFACTOR

O52063-citation-26390431
type: Citation Statement
scope: X-RAY CRYSTALLOGRAPHY (1.84 ANGSTROMS) IN COMPLEX WITH ZINC AND INHIBITOR OF WILD-TYPE AND MUTANTS ALA-219; ALA-249 AND ASP-304
scope: CATALYTIC ACTIVITY
scope: SUBUNIT
scope: BIOPHYSICOCHEMICAL PROPERTIES
scope: MUTAGENESIS OF LYS-65; GLN-160; ASP-188; HIS-219; HIS-249; ASP-303; ASN-304 AND TRP-309
scope: ACTIVE SITE

2QT3
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.24
chain sequence mapping: PDB 2QT3 tt1tt403

PDB 2QT3 tt1tt403
chain: A/B=1-403

4CQB
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 1.84
chain sequence mapping: PDB 4CQB tt1tt403

PDB 4CQB tt1tt403
chain: A/B=1-403

4CQC
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.20
chain sequence mapping: PDB 4CQC tt1tt403

PDB 4CQC tt1tt403
chain: A/B=1-403

4CQD
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.25
chain sequence mapping: PDB 4CQD tt1tt403

PDB 4CQD tt1tt403
chain: A/B=1-403

5AKQ
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.60
chain sequence mapping: PDB 5AKQ tt1tt403

PDB 5AKQ tt1tt403
chain: A/B=1-403

http://purl.uniprot.org/brenda/3.5.4.42
type: Resource
database: BRENDA
comment: 5085

cd01293
type: Resource
database: CDD
comment: Bact_CD
signature sequence match: CDD cd01293 match 1

PTHR32027:SF9
type: Resource
database: PANTHER
comment: BLL3847 PROTEIN
signature sequence match: PANTHER PTHR32027:SF9 match 1

PTHR32027
type: Resource
database: PANTHER
comment: CYTOSINE DEAMINASE
signature sequence match: PANTHER PTHR32027 match 1

SIPCDC1461A7CD2B744
type: Structured Name
full name: N-isopropylammelide isopropyl amidohydrolase
ec name: 3.5.4.42

pADP-1
type: Plasmid

atzC
type: Gene

SIP3A175567F31F5C15
type: Function Annotation
comment: Transforms N-isopropylammelide to cyanuric acid and isopropylamine.

SIP057E5B0A883BC275
type: Cofactor Annotation
comment: Binds 1 Zn(2+) ion per subunit.
cofactor: CHEBI_29105

SIP87E446E52DBFACD7
type: Activity Regulation Annotation
comment: Inhibited by N-ethylammeline, N-hydroxyethylammeline, N-isopropylammeline, ammeline and 2-amino-4hydroxy-1,3,5-s-triazine.

SIP42ADCBCCF64BD0A0
type: Kinetics Annotation
comment: kcat is 178 sec(-1) for N-methylammelide. kcat is 209 sec(-1) for N-ethylammelide. kcat is 80.6 sec(-1) for N-hydroxyethylammelide. kcat is 13.3 sec(-1) for N-isopropylammelide. kcat is 98.2 sec(-1) for N-cyclopropylammelide. kcat is 0.07 sec(-1) for N-t-butylammelide. kcat is 10.7 sec(-1) for N-dimethylammelide. kcat is 1.65 sec(-1) for ammelide.
measured affinity: 817 uM for N-methylammelide
measured affinity: 308 uM for N-ethylammelide
measured affinity: 3860 uM for N-hydroxyethylammelide
measured affinity: 406 uM for N-isopropylammelide
measured affinity: 580 uM for N-cyclopropylammelide
measured affinity: 299 uM for N-t-butylammelide
measured affinity: 155 uM for N-dimethylammelide
measured affinity: 1320 uM for ammelide

SIP668B5D36137DADF2
type: PH Dependence Annotation
comment: Optimum pH is 7.25.

SIPBFC15598407CD4EE
type: Subunit Annotation
comment: Homotetramer.

SIP32A94ED4DD3C5040
type: Similarity Annotation
comment: Belongs to the metallo-dependent hydrolases superfamily. N-acyl-D-amino-acid deacylase family.

PRO_0000182707
type: Chain Annotation
comment: N-isopropylammelide isopropyl amidohydrolase
mass: 44938
range: 22295012833637166tt1tt403

22295012833637166tt1
type: Position
type: ExactPosition
position: 1
reference: O52063-1

22295012833637166tt403
type: Position
type: ExactPosition
position: 403
reference: O52063-1

SIPAE354FD4DF2651B6
type: Active Site Annotation
comment: Proton donor/acceptor
range: 22295012833637166tt249tt249

22295012833637166tt249
type: Position
type: ExactPosition
position: 249
reference: O52063-1

22295012833637166tt60
type: Position
type: ExactPosition
position: 60
reference: O52063-1

22295012833637166tt62
type: Position
type: ExactPosition
position: 62
reference: O52063-1

22295012833637166tt217
type: Position
type: ExactPosition
position: 217
reference: O52063-1

22295012833637166tt303
type: Position
type: ExactPosition
position: 303
reference: O52063-1

SIP220ECFCEB7518DD8
type: Mutagenesis Annotation
comment: 30-fold increase in kcat with ammelide as substrate.
substitution: A
range: 22295012833637166tt65tt65

22295012833637166tt65
type: Position
type: ExactPosition
position: 65
reference: O52063-1

SIPBE63C3BD83D7ADD8
type: Mutagenesis Annotation
comment: 12-fold increase in kcat with ammelide as substrate.
substitution: R
range: 22295012833637166tt65tt65

SIP6A967D49D0D1FECF
type: Mutagenesis Annotation
comment: Almost no effect.
substitution: A
substitution: E
range: 22295012833637166tt160tt160

22295012833637166tt160
type: Position
type: ExactPosition
position: 160
reference: O52063-1

SIP3534FB9537CF31EC
type: Mutagenesis Annotation
comment: 5-fold increase in kcat with ammelide as substrate.
substitution: A
range: 22295012833637166tt188tt188

22295012833637166tt188
type: Position
type: ExactPosition
position: 188
reference: O52063-1

SIP3CBEE7630C64E214
type: Mutagenesis Annotation
comment: No effect.
substitution: N
range: 22295012833637166tt188tt188

SIPB1D7F9F2A1886655
type: Mutagenesis Annotation
comment: Almost no effect.
substitution: A
range: 22295012833637166tt219tt219

22295012833637166tt219
type: Position
type: ExactPosition
position: 219
reference: O52063-1

SIP46CE8380908D9B7D
type: Mutagenesis Annotation
comment: No activity.
substitution: A
range: 22295012833637166tt249tt249

SIP8A94E6FB2478DF76
type: Mutagenesis Annotation
comment: Almost no effect.
substitution: A
substitution: N
range: 22295012833637166tt303tt303

SIPEA305495CE3F3551
type: Mutagenesis Annotation
comment: Almost no effect.
substitution: A
range: 22295012833637166tt304tt304

22295012833637166tt304
type: Position
type: ExactPosition
position: 304
reference: O52063-1

SIPECD897AC7EE1E1CF
type: Mutagenesis Annotation
comment: 7-fold increase in kcat with ammelide as substrate.
substitution: D
range: 22295012833637166tt304tt304

SIPF494634A375A1D88
type: Mutagenesis Annotation
comment: Almost no effect.
substitution: A
substitution: F
range: 22295012833637166tt309tt309

22295012833637166tt309
type: Position
type: ExactPosition
position: 309
reference: O52063-1

22295012833637166tt4
type: Position
type: ExactPosition
position: 4
reference: O52063-1

22295012833637166tt14
type: Position
type: ExactPosition
position: 14
reference: O52063-1

22295012833637166tt15
type: Position
type: ExactPosition
position: 15
reference: O52063-1

22295012833637166tt18
type: Position
type: ExactPosition
position: 18
reference: O52063-1

22295012833637166tt19
type: Position
type: ExactPosition
position: 19
reference: O52063-1

22295012833637166tt26
type: Position
type: ExactPosition
position: 26
reference: O52063-1

22295012833637166tt29
type: Position
type: ExactPosition
position: 29
reference: O52063-1

22295012833637166tt36
type: Position
type: ExactPosition
position: 36
reference: O52063-1

22295012833637166tt41
type: Position
type: ExactPosition
position: 41
reference: O52063-1

22295012833637166tt46
type: Position
type: ExactPosition
position: 46
reference: O52063-1

22295012833637166tt51
type: Position
type: ExactPosition
position: 51
reference: O52063-1

22295012833637166tt54
type: Position
type: ExactPosition
position: 54
reference: O52063-1

22295012833637166tt56
type: Position
type: ExactPosition
position: 56
reference: O52063-1

22295012833637166tt61
type: Position
type: ExactPosition
position: 61
reference: O52063-1

22295012833637166tt63
type: Position
type: ExactPosition
position: 63
reference: O52063-1

22295012833637166tt66
type: Position
type: ExactPosition
position: 66
reference: O52063-1

22295012833637166tt69
type: Position
type: ExactPosition
position: 69
reference: O52063-1

22295012833637166tt71
type: Position
type: ExactPosition
position: 71
reference: O52063-1

22295012833637166tt74
type: Position
type: ExactPosition
position: 74
reference: O52063-1

22295012833637166tt76
type: Position
type: ExactPosition
position: 76
reference: O52063-1

22295012833637166tt79
type: Position
type: ExactPosition
position: 79
reference: O52063-1

22295012833637166tt84
type: Position
type: ExactPosition
position: 84
reference: O52063-1

22295012833637166tt97
type: Position
type: ExactPosition
position: 97
reference: O52063-1

22295012833637166tt100
type: Position
type: ExactPosition
position: 100
reference: O52063-1

22295012833637166tt116
type: Position
type: ExactPosition
position: 116
reference: O52063-1

22295012833637166tt119
type: Position
type: ExactPosition
position: 119
reference: O52063-1

22295012833637166tt127
type: Position
type: ExactPosition
position: 127
reference: O52063-1

22295012833637166tt130
type: Position
type: ExactPosition
position: 130
reference: O52063-1

22295012833637166tt134
type: Position
type: ExactPosition
position: 134
reference: O52063-1

22295012833637166tt135
type: Position
type: ExactPosition
position: 135
reference: O52063-1

22295012833637166tt146
type: Position
type: ExactPosition
position: 146
reference: O52063-1

22295012833637166tt147
type: Position
type: ExactPosition
position: 147
reference: O52063-1

22295012833637166tt150
type: Position
type: ExactPosition
position: 150
reference: O52063-1

22295012833637166tt152
type: Position
type: ExactPosition
position: 152
reference: O52063-1

22295012833637166tt158
type: Position
type: ExactPosition
position: 158
reference: O52063-1

22295012833637166tt163
type: Position
type: ExactPosition
position: 163
reference: O52063-1

22295012833637166tt165
type: Position
type: ExactPosition
position: 165
reference: O52063-1

22295012833637166tt169
type: Position
type: ExactPosition
position: 169
reference: O52063-1

22295012833637166tt179
type: Position
type: ExactPosition
position: 179
reference: O52063-1

22295012833637166tt182
type: Position
type: ExactPosition
position: 182
reference: O52063-1

22295012833637166tt185
type: Position
type: ExactPosition
position: 185
reference: O52063-1

22295012833637166tt189
type: Position
type: ExactPosition
position: 189
reference: O52063-1

22295012833637166tt193
type: Position
type: ExactPosition
position: 193
reference: O52063-1

22295012833637166tt196
type: Position
type: ExactPosition
position: 196
reference: O52063-1

22295012833637166tt209
type: Position
type: ExactPosition
position: 209
reference: O52063-1

22295012833637166tt213
type: Position
type: ExactPosition
position: 213
reference: O52063-1

22295012833637166tt218
type: Position
type: ExactPosition
position: 218
reference: O52063-1

22295012833637166tt222
type: Position
type: ExactPosition
position: 222
reference: O52063-1

22295012833637166tt238
type: Position
type: ExactPosition
position: 238
reference: O52063-1

22295012833637166tt245
type: Position
type: ExactPosition
position: 245
reference: O52063-1

22295012833637166tt252
type: Position
type: ExactPosition
position: 252
reference: O52063-1

22295012833637166tt255
type: Position
type: ExactPosition
position: 255
reference: O52063-1

22295012833637166tt258
type: Position
type: ExactPosition
position: 258
reference: O52063-1

22295012833637166tt271
type: Position
type: ExactPosition
position: 271
reference: O52063-1

22295012833637166tt274
type: Position
type: ExactPosition
position: 274
reference: O52063-1

22295012833637166tt278
type: Position
type: ExactPosition
position: 278
reference: O52063-1

22295012833637166tt279
type: Position
type: ExactPosition
position: 279
reference: O52063-1

22295012833637166tt281
type: Position
type: ExactPosition
position: 281
reference: O52063-1

22295012833637166tt288
type: Position
type: ExactPosition
position: 288
reference: O52063-1

22295012833637166tt293
type: Position
type: ExactPosition
position: 293
reference: O52063-1

22295012833637166tt297
type: Position
type: ExactPosition
position: 297
reference: O52063-1

22295012833637166tt301
type: Position
type: ExactPosition
position: 301
reference: O52063-1

22295012833637166tt306
type: Position
type: ExactPosition
position: 306
reference: O52063-1

22295012833637166tt317
type: Position
type: ExactPosition
position: 317
reference: O52063-1

22295012833637166tt328
type: Position
type: ExactPosition
position: 328
reference: O52063-1

22295012833637166tt333
type: Position
type: ExactPosition
position: 333
reference: O52063-1

22295012833637166tt343
type: Position
type: ExactPosition
position: 343
reference: O52063-1

22295012833637166tt345
type: Position
type: ExactPosition
position: 345
reference: O52063-1

22295012833637166tt351
type: Position
type: ExactPosition
position: 351
reference: O52063-1

22295012833637166tt354
type: Position
type: ExactPosition
position: 354
reference: O52063-1

22295012833637166tt356
type: Position
type: ExactPosition
position: 356
reference: O52063-1

22295012833637166tt358
type: Position
type: ExactPosition
position: 358
reference: O52063-1

22295012833637166tt360
type: Position
type: ExactPosition
position: 360
reference: O52063-1

22295012833637166tt367
type: Position
type: ExactPosition
position: 367
reference: O52063-1

22295012833637166tt374
type: Position
type: ExactPosition
position: 374
reference: O52063-1

22295012833637166tt375
type: Position
type: ExactPosition
position: 375
reference: O52063-1

22295012833637166tt381
type: Position
type: ExactPosition
position: 381
reference: O52063-1

22295012833637166tt385
type: Position
type: ExactPosition
position: 385
reference: O52063-1

22295012833637166tt390
type: Position
type: ExactPosition
position: 390
reference: O52063-1

22295012833637166tt393
type: Position
type: ExactPosition
position: 393
reference: O52063-1

22295012833637166tt397
type: Position
type: ExactPosition
position: 397
reference: O52063-1

SIP3BAF37F414AD6315
subclass of: CHEBI_29105
comment: catalytic

O52063-1
type: Simple Sequence
modified: 1998-06-01
version: 1
mass: 44938
value: MSKDFDLIIRNAYLSEKDSVYDIGIVGDRIIKIEAKIEGTVKDEIDAKGNLVSPGFVDAHTHMDKSFTSTGERLPKFWSRPYTRDAAIEDGLKYYKNATHEEIKRHVIEHAHMQVLHGTLYTRTHVDVDSVAKTKAVEAVLEAKEELKDLIDIQVVAFAQSGFFVDLESESLIRKSLDMGCDLVGGVDPATRENNVEGSLDLCFKLAKEYDVDIDYHIHDIGTVGVYSINRLAQKTIENGYKGRVTTSHAWCFADAPSEWLDEAIPLYKDSGMKFVTCFSSTPPTMPVIKLLEAGINLGCASDNIRDFWVPFGNGDMVQGALIETQRLELKTNRDLGLIWKMITSEGARVLGIEKNYGIEVGKKADLVVLNSLSPQWAIIDQAKRLCVIKNGRIIVKDEVIVA

http://purl.uniprot.org/uniprot/O52063#attribution-6106178181F20F82E77565EC02D7DEC5
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/12218024

http://purl.uniprot.org/uniprot/O52063#attribution-24AD162405D61B71D978298DFBAA9839
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/26390431

http://purl.uniprot.org/uniprot/O52063#attribution-EACB006B6E84CF67E3EE63315EA671F0
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/9422605

http://purl.uniprot.org/uniprot/O52063#attribution-F2F1DDB3D0A2C82EB6522CA9C1591FA0
manual: true
evidence: ECO_0000269
source: SIP79F1302C81D2BF93

http://purl.uniprot.org/uniprot/O52063#attribution-080ED3982FEC43F7C43A0689717F3D20
manual: true
evidence: ECO_0000305

Q936X3
type: Protein
reviewed: true
created: 2012-09-05
modified: 2023-05-03
version: 67
mnemonic: ATZE_PSESD
citation: http://purl.uniprot.org/citations/11544232
citation: http://purl.uniprot.org/citations/29523689
recommended name: SIP109E9AB64C60F48E
enzyme: http://purl.uniprot.org/enzyme/3.5.1.131
organism: http://purl.uniprot.org/taxonomy/47660
encoded in: pADP-1
annotation: SIPE74301899845DD37
annotation: SIP5EAB676C5B6C0588
annotation: SIPC1352B420ED1C58D
annotation: SIP6F52A7F39A3305F7
annotation: SIPF6CF7E8A224FF544
annotation: SIP162F6967C0DF6D1A
annotation: SIP88ADDDF5CA68F6D6
annotation: PRO_0000418774
annotation: SIP2D3EDC58A108783D
annotation: SIPBEE61D631B2A850F
annotation: SIPE1537BA7D74D76C0
annotation: SIP6B54B3E5ECE8420D
annotation: SIP8ED8FD8A72B8C308
annotation: SIPEE3CAE6FECEED302
annotation: SIP92B1C72248E8D1BF
annotation: SIPE7E3791391401DC2
annotation: SIPF37759CD12C00B00
annotation: SIPBF935BC3718CFA07
annotation: SIP5117AD23A9BA07BD
annotation: SIP87605D2738717D08
annotation: SIP719FCE39FC730B7E
annotation: SIP3F60315C87EAB9E9
annotation: SIP1D7D75EC6C52BC11
annotation: SIP4F0984860212C9BE
annotation: SIP841C37FE1418813B
annotation: SIP5550A0086B823BC6
annotation: SIP8CECB2605F0B1081
annotation: SIP4F79B8BB5C94DB91
annotation: SIPFCA6C0EBD596EF4F
annotation: SIP75E4326F04C96DB9
annotation: SIP6318DF92BDC06D67
annotation: SIPEE5202EC4C7DE61E
annotation: SIP2F4C04AFB5369E1A
annotation: SIP0CB0251EF5A08173
annotation: SIPD45297413FD558AE
annotation: SIP21E686578F258184
annotation: SIP832046C59BBABF31
annotation: SIP99F1AA6C3887BDA9
annotation: SIP130990C0750590FF
annotation: SIPEC987A07804E2F71
annotation: SIP65C5B4D21C2790E6
annotation: SIPEC089C64738E62CB
annotation: SIPF45BF8355D67E9A0
annotation: SIPCB71FBD743B7F77A
annotation: SIPF15F8ADBD6901487
annotation: SIP3C4FCF08BEBDDD02
annotation: SIPD3126C2CBCC8CD88
annotation: SIPFD578710740B242B
annotation: SIP444F90A0CDF1AFDC
annotation: SIP0141A3CCD4AFDA49
annotation: SIP479DB8F60984B7AD
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/2
classified with: http://purl.uniprot.org/keywords/378
classified with: http://purl.uniprot.org/keywords/614
classified with: GO_0018750
classified with: GO_0019381
sequence: Q936X3-1

Q936X3-citation-11544232
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
scope: PATHWAY
scope: GENE NAME

ADP
type: Strain

Q936X3-citation-29523689
type: Citation Statement
scope: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) IN COMPLEX WITH ATZG
scope: FUNCTION
scope: CATALYTIC ACTIVITY
scope: BIOPHYSICOCHEMICAL PROPERTIES
scope: SUBUNIT
scope: IDENTIFICATION BY MASS SPECTROMETRY

6C62
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 1.95
chain sequence mapping: PDB 6C62 tt1tt457
chain sequence mapping: PDB 6C62 tt1tt68

PDB 6C62 tt1tt457
chain: A/B=1-457

6C6G
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.10
chain sequence mapping: PDB 6C6G tt1tt457
chain sequence mapping: PDB 6C6G tt1tt68

PDB 6C6G tt1tt457
chain: A/B=1-457

http://purl.uniprot.org/brenda/3.5.1.131
type: Resource
database: BRENDA
comment: 14885

http://purl.uniprot.org/gene3d/3.90.1300.10
type: Resource
database: Gene3D
comment: Amidase signature (AS) domain
signature sequence match: Gene3D 3.90.1300.10 match 1

IPR000120
type: Resource
database: InterPro
comment: Amidase

IPR023631
type: Resource
database: InterPro
comment: Amidase_dom

IPR036928
type: Resource
database: InterPro
comment: AS_sf

IPR014087
type: Resource
database: InterPro
comment: Carboxybiuret_hydro_AtzE

PTHR11895:SF172
type: Resource
database: PANTHER
comment: GLUTAMYL-TRNA(GLN) AMIDOTRANSFERASE
signature sequence match: PANTHER PTHR11895:SF172 match 1

PTHR11895
type: Resource
database: PANTHER
comment: TRANSAMIDASE
signature sequence match: PANTHER PTHR11895 match 1

PF01425
type: Resource
database: Pfam
comment: Amidase
signature sequence match: Pfam PF01425 match 1

SSF75304
type: Resource
database: SUPFAM
comment: Amidase signature (AS) enzymes
signature sequence match: SUPFAM SSF75304 match 1

TIGR02715
type: Resource
database: TIGRFAMs
comment: amido_AtzE
signature sequence match: TIGRFAMs TIGR02715 match 1

SIP109E9AB64C60F48E
type: Structured Name
full name: 1-carboxybiuret hydrolase subunit AtzE
ec name: 3.5.1.131

pADP-1
type: Plasmid

atzE
type: Gene

SIPE74301899845DD37
type: Function Annotation
comment: Hydrolyzes 1-carboxybiuret to urea-1,3-dicarboxylate and NH(3).

SIPC1352B420ED1C58D
type: Kinetics Annotation
comment: kcat is 15.5 sec(-1) with 1-carboxybiuret as substrate. kcat is 9.4 sec(-1) with 1-nitrobiuret as substrate. kcat is 7 sec(-1) with 1-carboxymalonamide as substrate. kcat is 19 sec(-1) with succinamate as substrate.
measured affinity: 63 uM for 1-carboxybiuret
measured affinity: 785 uM for 1-nitrobiuret
measured affinity: 167 uM for 1-carboxymalonamide
measured affinity: 523 uM for succinamate

SIPF6CF7E8A224FF544
type: Subunit Annotation
comment: Heterotetramer consisting of 2 AtzE and 2 AtzG subunits.

SIP162F6967C0DF6D1A
type: Similarity Annotation
comment: Belongs to the amidase family.

SIP88ADDDF5CA68F6D6
type: Caution Annotation
comment: Was originally thought to use biuret as substrate (PubMed:11544232). But new evidence has shown that 1-carboxybiuret is the real substrate. The error occurred since 1-carboxybiuret hydrolyzes spontaneously to biuret (PubMed:29523689).

PRO_0000418774
type: Chain Annotation
comment: 1-carboxybiuret hydrolase subunit AtzE
mass: 48120
range: 22862365231428398tt1tt457

22862365231428398tt1
type: Position
type: ExactPosition
position: 1
reference: Q936X3-1

22862365231428398tt457
type: Position
type: ExactPosition
position: 457
reference: Q936X3-1

SIP2D3EDC58A108783D
type: Active Site Annotation
comment: Charge relay system
range: 22862365231428398tt74tt74

22862365231428398tt74
type: Position
type: ExactPosition
position: 74
reference: Q936X3-1

SIPBEE61D631B2A850F
type: Active Site Annotation
comment: Charge relay system
range: 22862365231428398tt150tt150

22862365231428398tt150
type: Position
type: ExactPosition
position: 150
reference: Q936X3-1

SIPE1537BA7D74D76C0
type: Active Site Annotation
comment: Acyl-ester intermediate
range: 22862365231428398tt174tt174

22862365231428398tt174
type: Position
type: ExactPosition
position: 174
reference: Q936X3-1

22862365231428398tt3
type: Position
type: ExactPosition
position: 3
reference: Q936X3-1

22862365231428398tt11
type: Position
type: ExactPosition
position: 11
reference: Q936X3-1

22862365231428398tt17
type: Position
type: ExactPosition
position: 17
reference: Q936X3-1

22862365231428398tt35
type: Position
type: ExactPosition
position: 35
reference: Q936X3-1

22862365231428398tt38
type: Position
type: ExactPosition
position: 38
reference: Q936X3-1

22862365231428398tt41
type: Position
type: ExactPosition
position: 41
reference: Q936X3-1

22862365231428398tt43
type: Position
type: ExactPosition
position: 43
reference: Q936X3-1

22862365231428398tt58
type: Position
type: ExactPosition
position: 58
reference: Q936X3-1

22862365231428398tt65
type: Position
type: ExactPosition
position: 65
reference: Q936X3-1

22862365231428398tt68
type: Position
type: ExactPosition
position: 68
reference: Q936X3-1

22862365231428398tt70
type: Position
type: ExactPosition
position: 70
reference: Q936X3-1

22862365231428398tt90
type: Position
type: ExactPosition
position: 90
reference: Q936X3-1

22862365231428398tt93
type: Position
type: ExactPosition
position: 93
reference: Q936X3-1

22862365231428398tt102
type: Position
type: ExactPosition
position: 102
reference: Q936X3-1

22862365231428398tt109
type: Position
type: ExactPosition
position: 109
reference: Q936X3-1

22862365231428398tt113
type: Position
type: ExactPosition
position: 113
reference: Q936X3-1

22862365231428398tt118
type: Position
type: ExactPosition
position: 118
reference: Q936X3-1

22862365231428398tt122
type: Position
type: ExactPosition
position: 122
reference: Q936X3-1

22862365231428398tt124
type: Position
type: ExactPosition
position: 124
reference: Q936X3-1

22862365231428398tt132
type: Position
type: ExactPosition
position: 132
reference: Q936X3-1

22862365231428398tt134
type: Position
type: ExactPosition
position: 134
reference: Q936X3-1

22862365231428398tt142
type: Position
type: ExactPosition
position: 142
reference: Q936X3-1

22862365231428398tt146
type: Position
type: ExactPosition
position: 146
reference: Q936X3-1

22862365231428398tt149
type: Position
type: ExactPosition
position: 149
reference: Q936X3-1

22862365231428398tt151
type: Position
type: ExactPosition
position: 151
reference: Q936X3-1

22862365231428398tt152
type: Position
type: ExactPosition
position: 152
reference: Q936X3-1

22862365231428398tt159
type: Position
type: ExactPosition
position: 159
reference: Q936X3-1

22862365231428398tt164
type: Position
type: ExactPosition
position: 164
reference: Q936X3-1

22862365231428398tt173
type: Position
type: ExactPosition
position: 173
reference: Q936X3-1

22862365231428398tt176
type: Position
type: ExactPosition
position: 176
reference: Q936X3-1

22862365231428398tt182
type: Position
type: ExactPosition
position: 182
reference: Q936X3-1

22862365231428398tt185
type: Position
type: ExactPosition
position: 185
reference: Q936X3-1

22862365231428398tt188
type: Position
type: ExactPosition
position: 188
reference: Q936X3-1

22862365231428398tt204
type: Position
type: ExactPosition
position: 204
reference: Q936X3-1

22862365231428398tt206
type: Position
type: ExactPosition
position: 206
reference: Q936X3-1

22862365231428398tt208
type: Position
type: ExactPosition
position: 208
reference: Q936X3-1

22862365231428398tt215
type: Position
type: ExactPosition
position: 215
reference: Q936X3-1

22862365231428398tt216
type: Position
type: ExactPosition
position: 216
reference: Q936X3-1

22862365231428398tt226
type: Position
type: ExactPosition
position: 226
reference: Q936X3-1

22862365231428398tt245
type: Position
type: ExactPosition
position: 245
reference: Q936X3-1

22862365231428398tt247
type: Position
type: ExactPosition
position: 247
reference: Q936X3-1

22862365231428398tt257
type: Position
type: ExactPosition
position: 257
reference: Q936X3-1

22862365231428398tt260
type: Position
type: ExactPosition
position: 260
reference: Q936X3-1

22862365231428398tt263
type: Position
type: ExactPosition
position: 263
reference: Q936X3-1

22862365231428398tt266
type: Position
type: ExactPosition
position: 266
reference: Q936X3-1

22862365231428398tt270
type: Position
type: ExactPosition
position: 270
reference: Q936X3-1

22862365231428398tt283
type: Position
type: ExactPosition
position: 283
reference: Q936X3-1

22862365231428398tt294
type: Position
type: ExactPosition
position: 294
reference: Q936X3-1

22862365231428398tt312
type: Position
type: ExactPosition
position: 312
reference: Q936X3-1

22862365231428398tt314
type: Position
type: ExactPosition
position: 314
reference: Q936X3-1

22862365231428398tt319
type: Position
type: ExactPosition
position: 319
reference: Q936X3-1

22862365231428398tt321
type: Position
type: ExactPosition
position: 321
reference: Q936X3-1

22862365231428398tt323
type: Position
type: ExactPosition
position: 323
reference: Q936X3-1

22862365231428398tt326
type: Position
type: ExactPosition
position: 326
reference: Q936X3-1

22862365231428398tt328
type: Position
type: ExactPosition
position: 328
reference: Q936X3-1

22862365231428398tt329
type: Position
type: ExactPosition
position: 329
reference: Q936X3-1

22862365231428398tt336
type: Position
type: ExactPosition
position: 336
reference: Q936X3-1

22862365231428398tt340
type: Position
type: ExactPosition
position: 340
reference: Q936X3-1

22862365231428398tt360
type: Position
type: ExactPosition
position: 360
reference: Q936X3-1

22862365231428398tt365
type: Position
type: ExactPosition
position: 365
reference: Q936X3-1

22862365231428398tt375
type: Position
type: ExactPosition
position: 375
reference: Q936X3-1

22862365231428398tt383
type: Position
type: ExactPosition
position: 383
reference: Q936X3-1

22862365231428398tt386
type: Position
type: ExactPosition
position: 386
reference: Q936X3-1

22862365231428398tt389
type: Position
type: ExactPosition
position: 389
reference: Q936X3-1

22862365231428398tt392
type: Position
type: ExactPosition
position: 392
reference: Q936X3-1

22862365231428398tt393
type: Position
type: ExactPosition
position: 393
reference: Q936X3-1

22862365231428398tt396
type: Position
type: ExactPosition
position: 396
reference: Q936X3-1

22862365231428398tt397
type: Position
type: ExactPosition
position: 397
reference: Q936X3-1

22862365231428398tt401
type: Position
type: ExactPosition
position: 401
reference: Q936X3-1

22862365231428398tt402
type: Position
type: ExactPosition
position: 402
reference: Q936X3-1

22862365231428398tt407
type: Position
type: ExactPosition
position: 407
reference: Q936X3-1

22862365231428398tt411
type: Position
type: ExactPosition
position: 411
reference: Q936X3-1

22862365231428398tt416
type: Position
type: ExactPosition
position: 416
reference: Q936X3-1

22862365231428398tt419
type: Position
type: ExactPosition
position: 419
reference: Q936X3-1

22862365231428398tt421
type: Position
type: ExactPosition
position: 421
reference: Q936X3-1

22862365231428398tt426
type: Position
type: ExactPosition
position: 426
reference: Q936X3-1

22862365231428398tt432
type: Position
type: ExactPosition
position: 432
reference: Q936X3-1

22862365231428398tt437
type: Position
type: ExactPosition
position: 437
reference: Q936X3-1

22862365231428398tt449
type: Position
type: ExactPosition
position: 449
reference: Q936X3-1

Q936X3-1
type: Simple Sequence
modified: 2001-12-01
version: 1
mass: 48120
value: MKTVEIIEGIASGRTSARDVCEEALATIGATDGLINAFTCRTVERARAEADAIDVRRARGEVLPPLAGLPYAVKNLFDIEGVTTLAGSKINRTLPPARADAVLVQRLKAAGAVLLGGLNMDEFAYGFTTENTHYGPTRNPHDTGRIAGGSSGGSGAAIAAGQVPLSLGSDTNGSIRVPASLCGVWGLKPTFGRLSRRGTYPFVHSIDHLGPLADSVEGLALAYDAMQGPDPLDPGCSASRIQPSVPVLSQGIAGLRIGVLGGWFRDNAGPAARAAVDVAALTLGASEVVMWPDAEIGRAAAFVITASEGGCLHLDDLRIRPQDFEPLSVDRFISGVLQPVAWYLRAQRFRRVYRDKVNALFRDWDILIAPATPISAPAIGTEWIEVNGTRHPCRPAMGLLTQPVSFAGCPVVAAPTWPGENDGMPIGVQLIAAPWNESLCLRAGKVLQDTGIARLKC

http://purl.uniprot.org/uniprot/Q936X3#attribution-F833C1DC6A67BEA500B005E8186B310A
creator: UniProtKB
manual: true
evidence: ECO_0000314
source: http://purl.uniprot.org/citations/11544232

http://purl.uniprot.org/uniprot/Q936X3#attribution-609619A7DF3E53CE5E48EDEF8B8F9DD1
manual: true
evidence: ECO_0000250

http://purl.uniprot.org/uniprot/Q936X3#attribution-60DD9D825BC595EE6491C75276A8F18E
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/11544232

http://purl.uniprot.org/uniprot/Q936X3#attribution-4E5BA653D210D9C410C6E2FF0E41147C
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/29523689

http://purl.uniprot.org/uniprot/Q936X3#attribution-412053D666D0C4FBB10EDE706B3285C9
manual: true
evidence: ECO_0000303
source: http://purl.uniprot.org/citations/11544232

http://purl.uniprot.org/uniprot/Q936X3#attribution-06C22B0C4D2C125B5133AEE93D47AD35
manual: true
evidence: ECO_0000303
source: http://purl.uniprot.org/citations/29523689

http://purl.uniprot.org/uniprot/Q936X3#attribution-080ED3982FEC43F7C43A0689717F3D20
manual: true
evidence: ECO_0000305

Q936X2
type: Protein
reviewed: true
created: 2012-09-05
modified: 2023-05-03
version: 76
mnemonic: ATZF_PSESD
citation: http://purl.uniprot.org/citations/11544232
citation: http://purl.uniprot.org/citations/15901697
recommended name: SIPD60574C252D61C71
enzyme: http://purl.uniprot.org/enzyme/3.5.1.54
organism: http://purl.uniprot.org/taxonomy/47660
encoded in: pADP-1
annotation: SIP3B7D5E122F4975DB
annotation: SIPA6EFA585FD0FE8CB
annotation: SIP3D9B68649F5F0201
annotation: SIPB3AC7A049DDBD2D7
annotation: SIP82DBD7572F4933FB
annotation: SIP5FC5C495A0425C28
annotation: SIP6AFF8567A144AD65
annotation: SIP5CDA4A9D4C3B5EF7
annotation: PRO_0000418775
annotation: SIP8E53FBE07AEF13F1
annotation: SIP311D68D32AA27758
annotation: SIPEA654C12476533CF
annotation: SIP5B49F312A7E7DA1D
annotation: SIPAFB4097438AC6DC4
annotation: SIP291966198726F6D8
annotation: SIP1851C9F8040D6B7D
annotation: SIPB5557BA13A44B2D6
annotation: SIP32F32B75AA8A1A6C
annotation: SIP3AF477A59D4998D0
annotation: SIPE16F9EBA1B877F5B
annotation: SIPCFD56536483213E1
annotation: SIP986BBE5738847ECF
annotation: SIP634E408AA601B87A
annotation: SIPE37DDCAF8CA11C6B
annotation: SIPB4E81732C6EC2EE9
annotation: SIP81D7884AE7E89D21
annotation: SIP3650A4DDC25AF952
annotation: SIP804B7E74D210006B
annotation: SIP988AACAFDBBFA8CC
annotation: SIP12C763327284DC6E
annotation: SIPB410B45AAE50E463
annotation: SIP6C5CF4F1E2D5A389
annotation: SIPBE0A7231F71BF38E
annotation: SIP0E4EB352AB03791B
annotation: SIP4D52DD8B51B589B5
annotation: SIPE3E7A17021BBBDDB
annotation: SIP0D68E60DD539455F
annotation: SIP9484C8E3818AAE62
annotation: SIP95EED7D6F5BBE9B8
annotation: SIP90422C4209D22AF2
annotation: SIP7D0A6C0D87C0E4C3
annotation: SIP95AEAE164ED3696F
annotation: SIPAA99626F5F7CAD79
annotation: SIP6799E1E010DB133F
annotation: SIPDE5E1953955B99C5
annotation: SIPE78A19FAFB59FED6
annotation: SIP22ED2CCF0F335F87
annotation: SIP3696FBF005EB54F8
annotation: SIP37243131CE4917E2
annotation: SIPCEBB4E9C31D8DB6C
annotation: SIPAADA3454C7AF4DAB
annotation: SIP9934BC0BA2A5FDA1
annotation: SIP0F62726BC891E558
annotation: SIP5B666BCA167E03F2
annotation: SIPE73EE9FA9FDC2C7C
annotation: SIP764F27885F9F6A1B
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/2
classified with: http://purl.uniprot.org/keywords/378
classified with: http://purl.uniprot.org/keywords/614
classified with: GO_0004039
classified with: GO_0019381
sequence: Q936X2-1

Q936X2-citation-11544232
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
scope: FUNCTION
scope: CATALYTIC ACTIVITY
scope: PATHWAY
scope: GENE NAME

ADP
type: Strain

Q936X2-citation-15901697
type: Citation Statement
scope: FUNCTION
scope: CATALYTIC ACTIVITY
scope: ACTIVITY REGULATION
scope: BIOPHYSICOCHEMICAL PROPERTIES
scope: PATHWAY
scope: SUBUNIT
scope: MUTAGENESIS OF LYS-91; SER-165 AND SER-189

4CP8
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.50
chain sequence mapping: PDB 4CP8 tt1tt467

PDB 4CP8 tt1tt467
chain: A/B/C/D/E/F=1-467

http://purl.uniprot.org/brenda/3.5.1.54
type: Resource
database: BRENDA
comment: 5085

http://purl.uniprot.org/gene3d/3.10.490.10
type: Resource
database: Gene3D
comment: Gamma-glutamyl cyclotransferase-like
signature sequence match: Gene3D 3.10.490.10 match 1

IPR014085
type: Resource
database: InterPro
comment: Allophanate_hydrolase

PTHR11895:SF169
type: Resource
database: PANTHER
comment: GLUTAMYL-TRNA(GLN) AMIDOTRANSFERASE
signature sequence match: PANTHER PTHR11895:SF169 match 1

TIGR02713
type: Resource
database: TIGRFAMs
comment: allophanate_hyd
signature sequence match: TIGRFAMs TIGR02713 match 1

SIPD60574C252D61C71
type: Structured Name
full name: Allophanate hydrolase
ec name: 3.5.1.54

pADP-1
type: Plasmid

atzF
type: Gene

SIP3B7D5E122F4975DB
type: Function Annotation
comment: Hydrolyzes allophanate to NH(3) and CO(2). Can also use malonamate, but with much lower efficiency.

SIP3D9B68649F5F0201
type: Activity Regulation Annotation
comment: Inhibited, in a time-dependent manner, by phenyl phosphorodiamidate, which forms a covalently modified Ser-189.

SIPB3AC7A049DDBD2D7
type: Kinetics Annotation
comment: kcat is 16.4 sec(-1) for allophanate. kcat is 0.1 sec(-1) for malonamate.
measured affinity: 1.5 mM for allophanate
measured affinity: 5.3 mM for malonamate

SIP82DBD7572F4933FB
type: PH Dependence Annotation
comment: Optimum pH is 9.5.

SIP6AFF8567A144AD65
type: Subunit Annotation
comment: Homotetramer.

SIP5CDA4A9D4C3B5EF7
type: Similarity Annotation
comment: Belongs to the amidase family.

PRO_0000418775
type: Chain Annotation
comment: Allophanate hydrolase
mass: 64060
range: 22862365231428142tt1tt605

22862365231428142tt1
type: Position
type: ExactPosition
position: 1
reference: Q936X2-1

22862365231428142tt605
type: Position
type: ExactPosition
position: 605
reference: Q936X2-1

SIP8E53FBE07AEF13F1
type: Active Site Annotation
comment: Charge relay system
range: 22862365231428142tt91tt91

22862365231428142tt91
type: Position
type: ExactPosition
position: 91
reference: Q936X2-1

SIP311D68D32AA27758
type: Active Site Annotation
comment: Charge relay system
range: 22862365231428142tt165tt165

22862365231428142tt165
type: Position
type: ExactPosition
position: 165
reference: Q936X2-1

SIPEA654C12476533CF
type: Active Site Annotation
comment: Acyl-ester intermediate
range: 22862365231428142tt189tt189

22862365231428142tt189
type: Position
type: ExactPosition
position: 189
reference: Q936X2-1

SIP5B49F312A7E7DA1D
type: Mutagenesis Annotation
comment: Loss of activity.
substitution: A
range: 22862365231428142tt91tt91

SIPAFB4097438AC6DC4
type: Mutagenesis Annotation
comment: Loss of activity.
substitution: A
range: 22862365231428142tt165tt165

SIP291966198726F6D8
type: Mutagenesis Annotation
comment: Loss of activity.
substitution: A
range: 22862365231428142tt189tt189

22862365231428142tt22
type: Position
type: ExactPosition
position: 22
reference: Q936X2-1

22862365231428142tt30
type: Position
type: ExactPosition
position: 30
reference: Q936X2-1

22862365231428142tt35
type: Position
type: ExactPosition
position: 35
reference: Q936X2-1

22862365231428142tt48
type: Position
type: ExactPosition
position: 48
reference: Q936X2-1

22862365231428142tt52
type: Position
type: ExactPosition
position: 52
reference: Q936X2-1

22862365231428142tt54
type: Position
type: ExactPosition
position: 54
reference: Q936X2-1

22862365231428142tt55
type: Position
type: ExactPosition
position: 55
reference: Q936X2-1

22862365231428142tt58
type: Position
type: ExactPosition
position: 58
reference: Q936X2-1

22862365231428142tt61
type: Position
type: ExactPosition
position: 61
reference: Q936X2-1

22862365231428142tt76
type: Position
type: ExactPosition
position: 76
reference: Q936X2-1

22862365231428142tt82
type: Position
type: ExactPosition
position: 82
reference: Q936X2-1

22862365231428142tt85
type: Position
type: ExactPosition
position: 85
reference: Q936X2-1

22862365231428142tt87
type: Position
type: ExactPosition
position: 87
reference: Q936X2-1

22862365231428142tt117
type: Position
type: ExactPosition
position: 117
reference: Q936X2-1

22862365231428142tt124
type: Position
type: ExactPosition
position: 124
reference: Q936X2-1

22862365231428142tt128
type: Position
type: ExactPosition
position: 128
reference: Q936X2-1

22862365231428142tt133
type: Position
type: ExactPosition
position: 133
reference: Q936X2-1

22862365231428142tt137
type: Position
type: ExactPosition
position: 137
reference: Q936X2-1

22862365231428142tt139
type: Position
type: ExactPosition
position: 139
reference: Q936X2-1

22862365231428142tt142
type: Position
type: ExactPosition
position: 142
reference: Q936X2-1

22862365231428142tt144
type: Position
type: ExactPosition
position: 144
reference: Q936X2-1

22862365231428142tt164
type: Position
type: ExactPosition
position: 164
reference: Q936X2-1

22862365231428142tt166
type: Position
type: ExactPosition
position: 166
reference: Q936X2-1

22862365231428142tt167
type: Position
type: ExactPosition
position: 167
reference: Q936X2-1

22862365231428142tt174
type: Position
type: ExactPosition
position: 174
reference: Q936X2-1

22862365231428142tt179
type: Position
type: ExactPosition
position: 179
reference: Q936X2-1

22862365231428142tt188
type: Position
type: ExactPosition
position: 188
reference: Q936X2-1

22862365231428142tt191
type: Position
type: ExactPosition
position: 191
reference: Q936X2-1

22862365231428142tt192
type: Position
type: ExactPosition
position: 192
reference: Q936X2-1

22862365231428142tt196
type: Position
type: ExactPosition
position: 196
reference: Q936X2-1

22862365231428142tt200
type: Position
type: ExactPosition
position: 200
reference: Q936X2-1

22862365231428142tt203
type: Position
type: ExactPosition
position: 203
reference: Q936X2-1

22862365231428142tt206
type: Position
type: ExactPosition
position: 206
reference: Q936X2-1

22862365231428142tt208
type: Position
type: ExactPosition
position: 208
reference: Q936X2-1

22862365231428142tt219
type: Position
type: ExactPosition
position: 219
reference: Q936X2-1

22862365231428142tt221
type: Position
type: ExactPosition
position: 221
reference: Q936X2-1

22862365231428142tt223
type: Position
type: ExactPosition
position: 223
reference: Q936X2-1

22862365231428142tt230
type: Position
type: ExactPosition
position: 230
reference: Q936X2-1

22862365231428142tt231
type: Position
type: ExactPosition
position: 231
reference: Q936X2-1

22862365231428142tt241
type: Position
type: ExactPosition
position: 241
reference: Q936X2-1

22862365231428142tt256
type: Position
type: ExactPosition
position: 256
reference: Q936X2-1

22862365231428142tt259
type: Position
type: ExactPosition
position: 259
reference: Q936X2-1

22862365231428142tt266
type: Position
type: ExactPosition
position: 266
reference: Q936X2-1

22862365231428142tt273
type: Position
type: ExactPosition
position: 273
reference: Q936X2-1

22862365231428142tt275
type: Position
type: ExactPosition
position: 275
reference: Q936X2-1

22862365231428142tt277
type: Position
type: ExactPosition
position: 277
reference: Q936X2-1

22862365231428142tt284
type: Position
type: ExactPosition
position: 284
reference: Q936X2-1

22862365231428142tt299
type: Position
type: ExactPosition
position: 299
reference: Q936X2-1

22862365231428142tt302
type: Position
type: ExactPosition
position: 302
reference: Q936X2-1

22862365231428142tt307
type: Position
type: ExactPosition
position: 307
reference: Q936X2-1

22862365231428142tt310
type: Position
type: ExactPosition
position: 310
reference: Q936X2-1

22862365231428142tt321
type: Position
type: ExactPosition
position: 321
reference: Q936X2-1

22862365231428142tt323
type: Position
type: ExactPosition
position: 323
reference: Q936X2-1

22862365231428142tt325
type: Position
type: ExactPosition
position: 325
reference: Q936X2-1

22862365231428142tt326
type: Position
type: ExactPosition
position: 326
reference: Q936X2-1

22862365231428142tt331
type: Position
type: ExactPosition
position: 331
reference: Q936X2-1

22862365231428142tt333
type: Position
type: ExactPosition
position: 333
reference: Q936X2-1

22862365231428142tt338
type: Position
type: ExactPosition
position: 338
reference: Q936X2-1

22862365231428142tt340
type: Position
type: ExactPosition
position: 340
reference: Q936X2-1

22862365231428142tt342
type: Position
type: ExactPosition
position: 342
reference: Q936X2-1

22862365231428142tt345
type: Position
type: ExactPosition
position: 345
reference: Q936X2-1

22862365231428142tt352
type: Position
type: ExactPosition
position: 352
reference: Q936X2-1

22862365231428142tt353
type: Position
type: ExactPosition
position: 353
reference: Q936X2-1

22862365231428142tt356
type: Position
type: ExactPosition
position: 356
reference: Q936X2-1

22862365231428142tt359
type: Position
type: ExactPosition
position: 359
reference: Q936X2-1

22862365231428142tt380
type: Position
type: ExactPosition
position: 380
reference: Q936X2-1

22862365231428142tt384
type: Position
type: ExactPosition
position: 384
reference: Q936X2-1

22862365231428142tt390
type: Position
type: ExactPosition
position: 390
reference: Q936X2-1

22862365231428142tt397
type: Position
type: ExactPosition
position: 397
reference: Q936X2-1

22862365231428142tt402
type: Position
type: ExactPosition
position: 402
reference: Q936X2-1

22862365231428142tt406
type: Position
type: ExactPosition
position: 406
reference: Q936X2-1

22862365231428142tt411
type: Position
type: ExactPosition
position: 411
reference: Q936X2-1

22862365231428142tt412
type: Position
type: ExactPosition
position: 412
reference: Q936X2-1

22862365231428142tt422
type: Position
type: ExactPosition
position: 422
reference: Q936X2-1

22862365231428142tt423
type: Position
type: ExactPosition
position: 423
reference: Q936X2-1

22862365231428142tt432
type: Position
type: ExactPosition
position: 432
reference: Q936X2-1

22862365231428142tt438
type: Position
type: ExactPosition
position: 438
reference: Q936X2-1

22862365231428142tt446
type: Position
type: ExactPosition
position: 446
reference: Q936X2-1

22862365231428142tt450
type: Position
type: ExactPosition
position: 450
reference: Q936X2-1

22862365231428142tt463
type: Position
type: ExactPosition
position: 463
reference: Q936X2-1

Q936X2-1
type: Simple Sequence
modified: 2001-12-01
version: 1
mass: 64060
value: MNDRAPHPERSGRVTPDHLTDLASYQAAYAAGTDAADVISDLYARIKEDGENPIWISLLPLESALAMLADAQQRKDKGEALPLFGIPFGVKDNIDVAGLPTTAGCTGFARTPRQHAFVVQRLVDAGAIPIGKTNLDQFATGLNGTRTPFGIPRCVFNENYVSGGSSSGSAVAVANGTVPFSLGTDTAGSGRIPAAFNNLVGLKPTKGLFSGSGLVPAARSLDCISVLAHTVDDALAVARVAAGYDADDAFSRKAGAAALTEKSWPRRFNFGVPAAEHRQFFGDAEAEALFNKAVRKLEEMGGTCISFDYTPFRQAAELLYAGPWVAERLAAIESLADEHPEVLHPVVRDIILSAKRMSAVDTFNGIYRLADLVRAAESTWEKIDVMLLPTAPTIYTVEDMLADPVRLNSNLGFYTNFVNLMDLSAIAVPAGFRTNGLPFGVTFIGRAFEDGAIASLGKAFVEHDLAKGNAATAAPPKDTVAIAVVGAHLSDQPLNHQLTESGGKLRATTRTAPGYALYALRDATPAKPGMLRDQNAVGSIEVEIWDLPVAGFGAFVSEIPAPLGIGTITLEDGSHVKGFLCEPHAIETALDITHYGGWRAYLAAQ

http://purl.uniprot.org/uniprot/Q936X2#attribution-A7C8C4200E1431F3213D30EEB14A48B2
creator: UniProtKB
manual: true
evidence: ECO_0000314
source: http://purl.uniprot.org/citations/15901697

http://purl.uniprot.org/uniprot/Q936X2#attribution-60DD9D825BC595EE6491C75276A8F18E
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/11544232

http://purl.uniprot.org/uniprot/Q936X2#attribution-2E602EBB8AF0A174F7D72BF750DAC2ED
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/15901697

http://purl.uniprot.org/uniprot/Q936X2#attribution-080ED3982FEC43F7C43A0689717F3D20
manual: true
evidence: ECO_0000305

http://purl.uniprot.org/uniprot/A0A384E126
type: Protein
reviewed: true
created: 2019-05-08
modified: 2023-05-03
version: 13
mnemonic: ATZG_PSESD
citation: http://purl.uniprot.org/citations/11544232
citation: http://purl.uniprot.org/citations/29523689
recommended name: SIP96C9830794073F0D
organism: http://purl.uniprot.org/taxonomy/47660
encoded in: pADP-1
annotation: SIPD02960A83F0F486A
annotation: SIP2C1009F1F5CC520A
annotation: SIP3F30783742B97FFB
annotation: PRO_0000447020
annotation: SIP524F5FD3B4577684
annotation: SIPE41C2653E2555620
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/2
classified with: http://purl.uniprot.org/keywords/614
classified with: GO_0019381
sequence: MTETEIFAYIEAASIAIGIPLEPARARAVAHHFSRTALLAEMLESVPLSPESELAEIYRPAPFPAEDI

A0A384E126-citation-11544232
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

ADP
type: Strain

A0A384E126-citation-29523689
type: Citation Statement
scope: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) IN COMPLEX WITH ATZE
scope: FUNCTION
scope: SUBUNIT
scope: IDENTIFICATION BY MASS SPECTROMETRY

PDB 6C62 tt1tt68
chain: C/D=1-68

PDB 6C6G tt1tt68
chain: C/D=1-68

IPR025148
type: Resource
database: InterPro
comment: AtzG-like

PF13318
type: Resource
database: Pfam
comment: AtzG-like
signature sequence match: Pfam PF13318 match 1

SIP96C9830794073F0D
type: Structured Name
full name: 1-carboxybiuret hydrolase subunit AtzG

pADP-1
type: Plasmid

atzG
type: Gene

SIPD02960A83F0F486A
type: Function Annotation
comment: Important for the activity of the AtzE subunit of 1-carboxybiuret hydrolase.

SIP3F30783742B97FFB
type: Subunit Annotation
comment: Heterotetramer consisting of 2 AtzE and 2 AtzG subunits.

PRO_0000447020
type: Chain Annotation
comment: 1-carboxybiuret hydrolase subunit AtzG
mass: 7422
range: -9218573199765104594tt1tt68

-9218573199765104594tt1
type: Position
type: ExactPosition
position: 1
reference: MTETEIFAYIEAASIAIGIPLEPARARAVAHHFSRTALLAEMLESVPLSPESELAEIYRPAPFPAEDI

-9218573199765104594tt68
type: Position
type: ExactPosition
position: 68
reference: MTETEIFAYIEAASIAIGIPLEPARARAVAHHFSRTALLAEMLESVPLSPESELAEIYRPAPFPAEDI

-9218573199765104594tt3
type: Position
type: ExactPosition
position: 3
reference: MTETEIFAYIEAASIAIGIPLEPARARAVAHHFSRTALLAEMLESVPLSPESELAEIYRPAPFPAEDI

-9218573199765104594tt17
type: Position
type: ExactPosition
position: 17
reference: MTETEIFAYIEAASIAIGIPLEPARARAVAHHFSRTALLAEMLESVPLSPESELAEIYRPAPFPAEDI

-9218573199765104594tt23
type: Position
type: ExactPosition
position: 23
reference: MTETEIFAYIEAASIAIGIPLEPARARAVAHHFSRTALLAEMLESVPLSPESELAEIYRPAPFPAEDI

-9218573199765104594tt44
type: Position
type: ExactPosition
position: 44
reference: MTETEIFAYIEAASIAIGIPLEPARARAVAHHFSRTALLAEMLESVPLSPESELAEIYRPAPFPAEDI

MTETEIFAYIEAASIAIGIPLEPARARAVAHHFSRTALLAEMLESVPLSPESELAEIYRPAPFPAEDI
type: Simple Sequence
modified: 2018-11-07
version: 1
mass: 7422

http://purl.uniprot.org/uniprot/A0A384E126#attribution-5D65A5BAC6DA9301E58C61A4C95046B6
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/29523689

http://purl.uniprot.org/uniprot/A0A384E126#attribution-06C22B0C4D2C125B5133AEE93D47AD35
manual: true
evidence: ECO_0000303
source: http://purl.uniprot.org/citations/29523689

http://purl.uniprot.org/uniprot/A0A384E126#attribution-07DBB181669244651E55C97D23DE5458
manual: true
evidence: ECO_0000305
source: http://purl.uniprot.org/citations/11544232

http://purl.uniprot.org/uniprot/A0A384E126#attribution-77ECFAC2859A85F2E200356A2B9DEF62
manual: true
evidence: ECO_0000312
source: 6C62

P80193
type: Protein
reviewed: true
created: 1993-04-01
modified: 2023-05-03
version: 85
mnemonic: BODG_PSESK
citation: http://purl.uniprot.org/citations/8504802
recommended name: SIP65CBB0DC505D6F22
alternative name: SIPB838CAC2D7DD63E1
alternative name: SIP232E50EC1518A8CA
enzyme: http://purl.uniprot.org/enzyme/1.14.11.1
organism: http://purl.uniprot.org/taxonomy/29440
annotation: SIPC05048A9ED1D16E4
annotation: SIP7F97848D00AD0F15
annotation: SIPE2493CA6E408B349
annotation: SIPFC995A8DA840EDAF
annotation: SIP8372E74C0666A623
annotation: SIPE1D16FD6C2D6E574
annotation: SIPF542588C00EF86D0
annotation: SIPEF00E12F2F52AD89
annotation: PRO_0000207089
annotation: SIP0922A8F93DA4F423
annotation: SIP96E55547C6B71ADF
annotation: SIP3AF6721E4200CC14
annotation: SIP6D9AAC385E93E829
annotation: SIP8CBED19C41AAAD56
annotation: SIP86165586DBBAB96B
annotation: SIPE0FF3748D000E8D5
annotation: SIP8845258F8C5396FD
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/124
classified with: http://purl.uniprot.org/keywords/963
classified with: http://purl.uniprot.org/keywords/223
classified with: http://purl.uniprot.org/keywords/903
classified with: http://purl.uniprot.org/keywords/408
classified with: http://purl.uniprot.org/keywords/479
classified with: http://purl.uniprot.org/keywords/862
classified with: GO_0005737
classified with: GO_0008336
classified with: GO_0005506
classified with: GO_0045329
sequence: P80193-1

P80193-citation-8504802
type: Citation Statement
scope: PROTEIN SEQUENCE

http://purl.uniprot.org/brenda/1.14.11.1
type: Resource
database: BRENDA
comment: 5085

cd00250
type: Resource
database: CDD
comment: CAS_like
signature sequence match: CDD cd00250 match 1

http://purl.uniprot.org/gene3d/3.60.130.10
type: Resource
database: Gene3D
comment: Clavaminate synthase-like
signature sequence match: Gene3D 3.60.130.10 match 1

IPR012775
type: Resource
database: InterPro
comment: GBBH

IPR010376
type: Resource
database: InterPro
comment: GBBH-like_N

IPR038492
type: Resource
database: InterPro
comment: GBBH-like_N_sf

IPR042098
type: Resource
database: InterPro
comment: TauD-like_sf

IPR003819
type: Resource
database: InterPro
comment: TauD/TfdA-like

PTHR10696
type: Resource
database: PANTHER
comment: GAMMA-BUTYROBETAINE HYDROXYLASE-RELATED
signature sequence match: PANTHER PTHR10696 match 1

PTHR10696:SF51
type: Resource
database: PANTHER
comment: TRIMETHYLLYSINE DIOXYGENASE, MITOCHONDRIAL
signature sequence match: PANTHER PTHR10696:SF51 match 1

PF06155
type: Resource
database: Pfam
comment: GBBH-like_N
signature sequence match: Pfam PF06155 match 1

PF02668
type: Resource
database: Pfam
comment: TauD
signature sequence match: Pfam PF02668 match 1

SSF51197
type: Resource
database: SUPFAM
comment: Clavaminate synthase-like
signature sequence match: SUPFAM SSF51197 match 1

TIGR02409
type: Resource
database: TIGRFAMs
comment: carnitine_bodg
signature sequence match: TIGRFAMs TIGR02409 match 1

SIP65CBB0DC505D6F22
type: Structured Name
full name: Gamma-butyrobetaine dioxygenase
ec name: 1.14.11.1

SIPB838CAC2D7DD63E1
type: Structured Name
full name: Gamma-butyrobetaine hydroxylase
short name: Gamma-BBH

SIP232E50EC1518A8CA
type: Structured Name
full name: Gamma-butyrobetaine,2-oxoglutarate dioxygenase

SIPC05048A9ED1D16E4
type: Function Annotation
comment: Catalyzes the formation of L-carnitine from gamma-butyrobetaine.

SIPE2493CA6E408B349
type: Cofactor Annotation
comment: Binds 1 Fe(2+) ion per subunit.
cofactor: CHEBI_29033

SIPE1D16FD6C2D6E574
type: Subunit Annotation
comment: Homodimer.

SIPEF00E12F2F52AD89
type: Similarity Annotation
comment: Belongs to the gamma-BBH/TMLD family.

PRO_0000207089
type: Chain Annotation
comment: Gamma-butyrobetaine dioxygenase
mass: 43322
range: 22579777772270382tt1tt383

22579777772270382tt1
type: Position
type: ExactPosition
position: 1
reference: P80193-1

22579777772270382tt383
type: Position
type: ExactPosition
position: 383
reference: P80193-1

22579777772270382tt46
type: Position
type: ExactPosition
position: 46
reference: P80193-1

22579777772270382tt48
type: Position
type: ExactPosition
position: 48
reference: P80193-1

22579777772270382tt51
type: Position
type: ExactPosition
position: 51
reference: P80193-1

22579777772270382tt91
type: Position
type: ExactPosition
position: 91
reference: P80193-1

22579777772270382tt209
type: Position
type: ExactPosition
position: 209
reference: P80193-1

22579777772270382tt211
type: Position
type: ExactPosition
position: 211
reference: P80193-1

22579777772270382tt350
type: Position
type: ExactPosition
position: 350
reference: P80193-1

SIP8845258F8C5396FD
type: Natural Variant Annotation
comment: In 50% of the chains.
range: 22579777772270382tt1tt1

SIP39D6F52CB3C50ABA
subclass of: CHEBI_24875
comment: catalytic

P80193-1
type: Simple Sequence
modified: 1993-04-01
version: 1
mass: 43322
value: NAIADYRTFPLISPLASAASFASGVSVTWADGRVSPFHNLWLRDNCPCGDCVYEVTREQVFLVADVPEDIQVQAVTIGDDGRLVVQWDDGHASAYHPGWLRAHAYDAQSLAEREAARPHKHRWMQGLSLPVYDHGAVMQDDDTLLEWLLAVRDVGLTQLHGVPTEPGALIPLAKRISFIRESNFGVLFDVRSKADADSNAYTAFNLPLHTDLPTRELQPGLQFLHCLVNDATGGNSTFVDGFAIAEALRIEAPAAYRLLCETPVEFRNKDRHSDYRCTAPVIALDSSGEVREIRLANFLRAPFQMDAQRMPDYYLAYRRFIQMTREPRFCFTRRLEAGQLWCFDNRRVLHARDAFDPASGDRHFQGCYVDRDELLSRILVLQR

http://purl.uniprot.org/uniprot/P80193#attribution-609619A7DF3E53CE5E48EDEF8B8F9DD1
manual: true
evidence: ECO_0000250

http://purl.uniprot.org/uniprot/P80193#attribution-080ED3982FEC43F7C43A0689717F3D20
manual: true
evidence: ECO_0000305

Q52438
type: Protein
reviewed: true
created: 1998-07-15
modified: 2023-05-03
version: 90
mnemonic: BPHA1_PSES1
old mnemonic: BPA1_PSES1
citation: http://purl.uniprot.org/citations/8048958
recommended name: SIPA2AEEEBA7DB312AD
alternative name: SIP06137D0A1F2452C9
enzyme: http://purl.uniprot.org/enzyme/1.14.12.18
organism: http://purl.uniprot.org/taxonomy/307
annotation: SIP01D03B8AA712D150
annotation: SIPF2D101D838EC6979
annotation: SIPE759C12E3B16DA7B
annotation: SIP0A0CF39F0ADC5130
annotation: SIPE2F6CE910E998203
annotation: SIPDCDFEE77EA44CC72
annotation: PRO_0000085049
annotation: SIP4C2D4E8BCB3C0DCD
annotation: SIPE3E4022FF86D9789
annotation: SIP7C14B195638DDE23
annotation: SIP6519F34676AA2DD3
annotation: SIP4A57C31E2A622D51
annotation: SIPA1403E4761424108
annotation: SIP867CB5A57F56DEF9
existence: Inferred from Homology Existence
classified with: http://purl.uniprot.org/keywords/1
classified with: http://purl.uniprot.org/keywords/58
classified with: http://purl.uniprot.org/keywords/223
classified with: http://purl.uniprot.org/keywords/520
classified with: GO_0051537
classified with: GO_0018687
classified with: GO_0005506
classified with: GO_0019439
sequence: Q52438-1

Q52438-citation-8048958
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

http://purl.uniprot.org/brenda/1.14.12.18
type: Resource
database: BRENDA
comment: 5085

cd08881
type: Resource
database: CDD
comment: RHO_alpha_C_NDO-like
signature sequence match: CDD cd08881 match 1

cd03472
type: Resource
database: CDD
comment: Rieske_RO_Alpha_BPDO_like
signature sequence match: CDD cd03472 match 1

http://purl.uniprot.org/gene3d/2.102.10.10
type: Resource
database: Gene3D
comment: Rieske [2Fe-2S] iron-sulphur domain
signature sequence match: Gene3D 2.102.10.10 match 1

IPR043266
type: Resource
database: InterPro
comment: RHO_NdoB-like_C

IPR017941
type: Resource
database: InterPro
comment: Rieske_2Fe-2S

IPR036922
type: Resource
database: InterPro
comment: Rieske_2Fe-2S_sf

IPR015881
type: Resource
database: InterPro
comment: Ring-hydroxy_dOase_2Fe2S_BS

IPR015879
type: Resource
database: InterPro
comment: Ring_hydroxy_dOase_asu_C_dom

IPR001663
type: Resource
database: InterPro
comment: Rng_hydr_dOase-A

PTHR43756:SF1
type: Resource
database: PANTHER
comment: 3-PHENYLPROPIONATE/CINNAMIC ACID DIOXYGENASE SUBUNIT ALPHA
signature sequence match: PANTHER PTHR43756:SF1 match 1

PTHR43756
type: Resource
database: PANTHER
comment: CHOLINE MONOOXYGENASE, CHLOROPLASTIC
signature sequence match: PANTHER PTHR43756 match 1

PF00355
type: Resource
database: Pfam
comment: Rieske
signature sequence match: Pfam PF00355 match 1

PF00848
type: Resource
database: Pfam
comment: Ring_hydroxyl_A
signature sequence match: Pfam PF00848 match 1

PR00090
type: Resource
database: PRINTS
comment: RNGDIOXGNASE

SSF55961
type: Resource
database: SUPFAM
comment: Bet v1-like
signature sequence match: SUPFAM SSF55961 match 1

SSF50022
type: Resource
database: SUPFAM
comment: ISP domain
signature sequence match: SUPFAM SSF50022 match 1

PS51296
type: Resource
database: PROSITE
comment: RIESKE
signature sequence match: PROSITE PS51296 match 1

PS00570
type: Resource
database: PROSITE
comment: RING_HYDROXYL_ALPHA
signature sequence match: PROSITE PS00570 match 1

SIPA2AEEEBA7DB312AD
type: Structured Name
full name: Biphenyl dioxygenase subunit alpha
ec name: 1.14.12.18

SIP06137D0A1F2452C9
type: Structured Name
full name: Biphenyl 2,3-dioxygenase

bphA1
type: Gene

SIPF2D101D838EC6979
type: Cofactor Annotation
comment: Binds 1 [2Fe-2S] cluster per subunit.
cofactor: CHEBI_190135

SIPE759C12E3B16DA7B
type: Cofactor Annotation
comment: Binds 1 Fe cation per subunit.
cofactor: CHEBI_24875

SIPE2F6CE910E998203
type: Subunit Annotation
comment: Heterohexamer consisting of 3 BphA1 subunits and 3 BphA2 subunits. A ferredoxin (BphA3) and a ferredoxin reductase (BphA4) must be present to obtain activity (By similarity).

SIPDCDFEE77EA44CC72
type: Similarity Annotation
comment: Belongs to the bacterial ring-hydroxylating dioxygenase alpha subunit family.

PRO_0000085049
type: Chain Annotation
comment: Biphenyl dioxygenase subunit alpha
mass: 51876
range: 22857962853972014tt1tt458

22857962853972014tt1
type: Position
type: ExactPosition
position: 1
reference: Q52438-1

22857962853972014tt458
type: Position
type: ExactPosition
position: 458
reference: Q52438-1

SIP4C2D4E8BCB3C0DCD
type: Domain Extent Annotation
comment: Rieske
range: 22857962853972014tt59tt157

22857962853972014tt59
type: Position
type: ExactPosition
position: 59
reference: Q52438-1

22857962853972014tt157
type: Position
type: ExactPosition
position: 157
reference: Q52438-1

22857962853972014tt101
type: Position
type: ExactPosition
position: 101
reference: Q52438-1

22857962853972014tt103
type: Position
type: ExactPosition
position: 103
reference: Q52438-1

22857962853972014tt121
type: Position
type: ExactPosition
position: 121
reference: Q52438-1

22857962853972014tt124
type: Position
type: ExactPosition
position: 124
reference: Q52438-1

22857962853972014tt234
type: Position
type: ExactPosition
position: 234
reference: Q52438-1

22857962853972014tt240
type: Position
type: ExactPosition
position: 240
reference: Q52438-1

Q52438-1
type: Simple Sequence
modified: 1996-11-01
version: 1
mass: 51876
value: MGIYSEREVQAVPMTFKRRWTDEAIRALVDQDKGLIDPRIYADQDLYEIELERIFARSWLLLGHEAHIPKTGDYLTTYMGEDPVIMVRQKDGSIKVFLNQCRHRGMRICRSDAGNAKAFTCTYHGWAYDIAGNLVNVPYEKEAFCDKKEGDCGFDKADWGPLQARVETYKGLIFANWDAEAPDLKTYLSDAMPYMDVMLDRTEAGTTVVGGMQKWVIPCNWKFAAEQFCSDMYHAGTMAHLSGVLSSLPPEMDLTQVQMSKNGSQFRAAWGGHGSGWFINDAAILMAVMGPKITQYWTQGPAAEKAAKRLNQMPTQTMFGQHMTVFPTCSFLPGINTIRSWHPRGPNEVECGPSWSSMPMRPEDIKEEFRRQNIRTFNAGGTFEQDDGENWVEIQRGLRGHKAKSAPLCAQMGLNVPNKSNPDFPGKTAYVYAEEAARGMYHHWARMMSEPNWETLKP

http://purl.uniprot.org/uniprot/Q52438#attribution-609619A7DF3E53CE5E48EDEF8B8F9DD1
manual: true
evidence: ECO_0000250

http://purl.uniprot.org/uniprot/Q52438#attribution-FDD5DF45D0B154FC8B4221196591E241
manual: true
evidence: ECO_0000255
source: PRU00628

http://purl.uniprot.org/uniprot/Q52438#attribution-080ED3982FEC43F7C43A0689717F3D20
manual: true
evidence: ECO_0000305

Q52439
type: Protein
reviewed: true
created: 1998-07-15
modified: 2023-05-03
version: 72
mnemonic: BPHA2_PSES1
old mnemonic: BPA2_PSES1
citation: http://purl.uniprot.org/citations/8048958
recommended name: SIP4AF980A9BED57661
alternative name: SIP06137D0A1F2452C9
enzyme: http://purl.uniprot.org/enzyme/1.14.12.18
organism: http://purl.uniprot.org/taxonomy/307
annotation: SIPE3928CAC77EF96C8
annotation: SIP0A3519AD0B7C950C
annotation: SIP21E62A48F23C5235
annotation: SIP6E724EBA10764501
annotation: SIPB098E2E3119F06A8
annotation: PRO_0000085070
existence: Inferred from Homology Existence
classified with: http://purl.uniprot.org/keywords/58
classified with: http://purl.uniprot.org/keywords/223
classified with: http://purl.uniprot.org/keywords/520
classified with: GO_0018687
classified with: GO_0019439
classified with: GO_0071704
sequence: MTRVAMAQKTAELIQEFAWPAQPVSPQLQHEVEQFYYREAQLLDHHNYNAWFDLLEQDIHYWMPIRTTRIKRENDKEYIPAGANAHFDENHETMRGRIRQRTSELNWAEDPLSRTRHIVSNVIVRETDTPATLEVASAFLVYRNRLERQVDIFAGERRDVLRRANNGLGFKIARRTILIDQSTILANNLSVFF

Q52439-citation-8048958
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

cd00667
type: Resource
database: CDD
comment: ring_hydroxylating_dioxygenases_beta
signature sequence match: CDD cd00667 match 1

IPR032710
type: Resource
database: InterPro
comment: NTF2-like_dom_sf

IPR000391
type: Resource
database: InterPro
comment: Rng_hydr_dOase-bsu

PTHR41534:SF2
type: Resource
database: PANTHER
comment: 3-PHENYLPROPIONATE/CINNAMIC ACID DIOXYGENASE SUBUNIT BETA
signature sequence match: PANTHER PTHR41534:SF2 match 1

PTHR41534
type: Resource
database: PANTHER
comment: BLR3401 PROTEIN
signature sequence match: PANTHER PTHR41534 match 1

PF00866
type: Resource
database: Pfam
comment: Ring_hydroxyl_B
signature sequence match: Pfam PF00866 match 1

SSF54427
type: Resource
database: SUPFAM
comment: NTF2-like
signature sequence match: SUPFAM SSF54427 match 1

SIP4AF980A9BED57661
type: Structured Name
full name: Biphenyl dioxygenase subunit beta
ec name: 1.14.12.18

SIP06137D0A1F2452C9
type: Structured Name
full name: Biphenyl 2,3-dioxygenase

bphA2
type: Gene

SIPE3928CAC77EF96C8
type: Function Annotation
comment: The beta subunit may be responsible for the substrate specificity of the enzyme.

SIP6E724EBA10764501
type: Subunit Annotation
comment: Heterohexamer consisting of 3 BphA1 subunits and 3 BphA2 subunits. A ferredoxin (BphA3) and a ferredoxin reductase (BphA4) must be present to obtain activity.

SIPB098E2E3119F06A8
type: Similarity Annotation
comment: Belongs to the bacterial ring-hydroxylating dioxygenase beta subunit family.

PRO_0000085070
type: Chain Annotation
comment: Biphenyl dioxygenase subunit beta
mass: 22779
range: 22857962853972270tt1tt193

22857962853972270tt1
type: Position
type: ExactPosition
position: 1
reference: MTRVAMAQKTAELIQEFAWPAQPVSPQLQHEVEQFYYREAQLLDHHNYNAWFDLLEQDIHYWMPIRTTRIKRENDKEYIPAGANAHFDENHETMRGRIRQRTSELNWAEDPLSRTRHIVSNVIVRETDTPATLEVASAFLVYRNRLERQVDIFAGERRDVLRRANNGLGFKIARRTILIDQSTILANNLSVFF

22857962853972270tt193
type: Position
type: ExactPosition
position: 193
reference: MTRVAMAQKTAELIQEFAWPAQPVSPQLQHEVEQFYYREAQLLDHHNYNAWFDLLEQDIHYWMPIRTTRIKRENDKEYIPAGANAHFDENHETMRGRIRQRTSELNWAEDPLSRTRHIVSNVIVRETDTPATLEVASAFLVYRNRLERQVDIFAGERRDVLRRANNGLGFKIARRTILIDQSTILANNLSVFF

MTRVAMAQKTAELIQEFAWPAQPVSPQLQHEVEQFYYREAQLLDHHNYNAWFDLLEQDIHYWMPIRTTRIKRENDKEYIPAGANAHFDENHETMRGRIRQRTSELNWAEDPLSRTRHIVSNVIVRETDTPATLEVASAFLVYRNRLERQVDIFAGERRDVLRRANNGLGFKIARRTILIDQSTILANNLSVFF
type: Simple Sequence
modified: 1996-11-01
version: 1
mass: 22779

http://purl.uniprot.org/uniprot/Q52439#attribution-080ED3982FEC43F7C43A0689717F3D20
manual: true
evidence: ECO_0000305

Q52440
type: Protein
reviewed: true
created: 1998-07-15
modified: 2023-05-03
version: 72
mnemonic: BPHA3_PSES1
old mnemonic: BPA3_PSES1
citation: http://purl.uniprot.org/citations/8048958
recommended name: SIPA00E1541C59154DD
organism: http://purl.uniprot.org/taxonomy/307
annotation: SIP6C389D1C94C7E21E
annotation: SIPCA96F91F52CDB60B
annotation: SIP0E0BF7CF85DB62EC
annotation: PRO_0000201687
annotation: SIPE89C69134FED7653
annotation: SIPFFF74F3B327DFA80
annotation: SIPCF09F016D26AF145
annotation: SIP8787E7D4FFDAE783
annotation: SIP64B92165CF716D8A
annotation: SIP678C4DEA3AC31CAE
annotation: SIP554BC89334C3D751
annotation: SIP9D5FC3E7FB892863
annotation: SIP123EA2C8C706A571
annotation: SIPB2F4587BCAA9C4EC
annotation: SIP67F7696A73D67636
annotation: SIP397092E8DD897F38
annotation: SIPCA8F5405EC143DF8
annotation: SIP4C511418913FDCA8
annotation: SIP8E8F28FC9E2CDA45
annotation: SIP533AB6680D155864
annotation: SIP46070EF209A0256B
annotation: SIP1894208C607B51B9
annotation: SIPBC0391A287C36439
annotation: SIP7FB72DB91C81916B
annotation: SIP11F8BF43E4907102
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/1
classified with: http://purl.uniprot.org/keywords/2
classified with: http://purl.uniprot.org/keywords/58
classified with: http://purl.uniprot.org/keywords/249
classified with: GO_0051537
classified with: GO_0046872
classified with: GO_0019439
sequence: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

Q52440-citation-8048958
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

2E4P
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.00
chain sequence mapping: PDB 2E4P tt1tt109

PDB 2E4P tt1tt109
chain: A/B=1-109

2E4Q
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 1.80
chain sequence mapping: PDB 2E4Q tt1tt109

PDB 2E4Q tt1tt109
chain: A/C=1-109

2YVJ
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 1.90
chain sequence mapping: PDB 2YVJ tt1tt109

PDB 2YVJ tt1tt109
chain: B=1-109

cd03528
type: Resource
database: CDD
comment: Rieske_RO_ferredoxin
signature sequence match: CDD cd03528 match 1

PTHR21496:SF23
type: Resource
database: PANTHER
comment: 3-PHENYLPROPIONATE/CINNAMIC ACID DIOXYGENASE FERREDOXIN SUBUNIT
signature sequence match: PANTHER PTHR21496:SF23 match 1

PTHR21496
type: Resource
database: PANTHER
comment: FERREDOXIN-RELATED
signature sequence match: PANTHER PTHR21496 match 1

SIPA00E1541C59154DD
type: Structured Name
full name: Biphenyl dioxygenase ferredoxin subunit

bphA3
type: Gene

SIP6C389D1C94C7E21E
type: Function Annotation
comment: This protein seems to be a 2Fe-2S ferredoxin.

SIPCA96F91F52CDB60B
type: Subunit Annotation
comment: This dioxygenase system consists of four proteins: the two subunits of the hydroxylase component (BphA1 and BphA2), a ferredoxin (BphA3) and a ferredoxin reductase (BphA4).

SIP0E0BF7CF85DB62EC
type: Similarity Annotation
comment: Belongs to the bacterial ring-hydroxylating dioxygenase ferredoxin component family.

PRO_0000201687
type: Chain Annotation
comment: Biphenyl dioxygenase ferredoxin subunit
mass: 11913
range: 22857962854035502tt1tt109

22857962854035502tt1
type: Position
type: ExactPosition
position: 1
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt109
type: Position
type: ExactPosition
position: 109
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

SIPE89C69134FED7653
type: Domain Extent Annotation
comment: Rieske
range: 22857962854035502tt4tt100

22857962854035502tt4
type: Position
type: ExactPosition
position: 4
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt100
type: Position
type: ExactPosition
position: 100
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt43
type: Position
type: ExactPosition
position: 43
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt45
type: Position
type: ExactPosition
position: 45
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt63
type: Position
type: ExactPosition
position: 63
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt66
type: Position
type: ExactPosition
position: 66
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt8
type: Position
type: ExactPosition
position: 8
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt9
type: Position
type: ExactPosition
position: 9
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt11
type: Position
type: ExactPosition
position: 11
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt17
type: Position
type: ExactPosition
position: 17
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt22
type: Position
type: ExactPosition
position: 22
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt25
type: Position
type: ExactPosition
position: 25
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt32
type: Position
type: ExactPosition
position: 32
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt35
type: Position
type: ExactPosition
position: 35
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt42
type: Position
type: ExactPosition
position: 42
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt44
type: Position
type: ExactPosition
position: 44
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt46
type: Position
type: ExactPosition
position: 46
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt51
type: Position
type: ExactPosition
position: 51
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt53
type: Position
type: ExactPosition
position: 53
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt55
type: Position
type: ExactPosition
position: 55
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt57
type: Position
type: ExactPosition
position: 57
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt60
type: Position
type: ExactPosition
position: 60
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt62
type: Position
type: ExactPosition
position: 62
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt64
type: Position
type: ExactPosition
position: 64
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt69
type: Position
type: ExactPosition
position: 69
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt71
type: Position
type: ExactPosition
position: 71
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt72
type: Position
type: ExactPosition
position: 72
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt74
type: Position
type: ExactPosition
position: 74
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt77
type: Position
type: ExactPosition
position: 77
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt81
type: Position
type: ExactPosition
position: 81
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt91
type: Position
type: ExactPosition
position: 91
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt94
type: Position
type: ExactPosition
position: 94
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt97
type: Position
type: ExactPosition
position: 97
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt102
type: Position
type: ExactPosition
position: 102
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt104
type: Position
type: ExactPosition
position: 104
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA
type: Simple Sequence
modified: 1996-11-01
version: 1
mass: 11913

http://purl.uniprot.org/uniprot/Q52440#attribution-FDD5DF45D0B154FC8B4221196591E241
manual: true
evidence: ECO_0000255
source: PRU00628

http://purl.uniprot.org/uniprot/Q52440#attribution-080ED3982FEC43F7C43A0689717F3D20
manual: true
evidence: ECO_0000305

P50206
type: Protein
reviewed: true
created: 1996-10-01
modified: 2023-05-03
version: 86
mnemonic: BPHB_PSES1
citation: http://purl.uniprot.org/citations/8048958
recommended name: SIP4758C8A635B7CAA5
alternative name: SIP6524A4412653F9C4
alternative name: SIP129ACE5A97F83313
alternative name: SIPE426E9E77F06E505
alternative name: SIPCE90861E864593F0
enzyme: http://purl.uniprot.org/enzyme/1.3.1.56
organism: http://purl.uniprot.org/taxonomy/307
annotation: SIP1BC43856A96AA0F5
annotation: SIPE1E78113522DDC9B
annotation: SIP7559DB2C2ACF8539
annotation: PRO_0000054534
annotation: SIP8FE670C5D012F316
annotation: SIP6B057CC6B9CB1972
annotation: SIPE48136005C37490C
existence: Inferred from Homology Existence
classified with: http://purl.uniprot.org/keywords/58
classified with: http://purl.uniprot.org/keywords/520
classified with: http://purl.uniprot.org/keywords/560
classified with: GO_0018509
classified with: GO_0019439
sequence: P50206-1

P50206-citation-8048958
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

cd05348
type: Resource
database: CDD
comment: BphB-like_SDR_c
signature sequence match: CDD cd05348 match 1

IPR017711
type: Resource
database: InterPro
comment: BphB_TodD

IPR020904
type: Resource
database: InterPro
comment: Sc_DH/Rdtase_CS

IPR002347
type: Resource
database: InterPro
comment: SDR_fam

PTHR43008
type: Resource
database: PANTHER
comment: BENZIL REDUCTASE
signature sequence match: PANTHER PTHR43008 match 1

PTHR43008:SF4
type: Resource
database: PANTHER
comment: CHAIN DEHYDROGENASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G08710)-RELATED
signature sequence match: PANTHER PTHR43008:SF4 match 1

PF00106
type: Resource
database: Pfam
comment: adh_short
signature sequence match: Pfam PF00106 match 1

PR00081
type: Resource
database: PRINTS
comment: GDHRDH

PR00080
type: Resource
database: PRINTS
comment: SDRFAMILY

TIGR03325
type: Resource
database: TIGRFAMs
comment: BphB_TodD
signature sequence match: TIGRFAMs TIGR03325 match 1

PS00061
type: Resource
database: PROSITE
comment: ADH_SHORT
signature sequence match: PROSITE PS00061 match 1

SIP4758C8A635B7CAA5
type: Structured Name
full name: Cis-2,3-dihydrobiphenyl-2,3-diol dehydrogenase
ec name: 1.3.1.56

SIP6524A4412653F9C4
type: Structured Name
full name: 2,3-dihydro-2,3-dihydroxybiphenyl dehydrogenase

SIP129ACE5A97F83313
type: Structured Name
full name: 2,3-dihydroxy-4-phenylhexa-4,6-diene dehydrogenase

SIPE426E9E77F06E505
type: Structured Name
full name: Biphenyl-2,3-dihydro-2,3-diol dehydrogenase

SIPCE90861E864593F0
type: Structured Name
full name: Biphenyl-cis-diol dehydrogenase

bphB
type: Gene

SIP7559DB2C2ACF8539
type: Similarity Annotation
comment: Belongs to the short-chain dehydrogenases/reductases (SDR) family.

PRO_0000054534
type: Chain Annotation
comment: Cis-2,3-dihydrobiphenyl-2,3-diol dehydrogenase
mass: 28933
range: 22576479253575214tt1tt276

22576479253575214tt1
type: Position
type: ExactPosition
position: 1
reference: P50206-1

22576479253575214tt276
type: Position
type: ExactPosition
position: 276
reference: P50206-1

SIP8FE670C5D012F316
type: Active Site Annotation
comment: Proton acceptor
range: 22576479253575214tt155tt155

22576479253575214tt155
type: Position
type: ExactPosition
position: 155
reference: P50206-1

22576479253575214tt9
type: Position
type: ExactPosition
position: 9
reference: P50206-1

22576479253575214tt33
type: Position
type: ExactPosition
position: 33
reference: P50206-1

22576479253575214tt142
type: Position
type: ExactPosition
position: 142
reference: P50206-1

substrate

P50206-1
type: Simple Sequence
modified: 1996-10-01
version: 1
mass: 28933
value: MQLNNEVALVTGGGSGLGRAIVDRFVAEGARVAVLDKSAARLQELQAAHGAKVLGIEGDVRVLADHQKAARECVAAFGKIDCLIPNAGIWDYSMPLVDIPDDRIDAAFDEVFHINVKGYLLAVKACLPALVQSRGSVVFTISNAGFYPNGGGPLYTGAKHAVVGMVRELAYELAPHVRVNGVAPGGMSTDLRGPASLGMANQAISSVPLGEMLTSVLPVGRMPVRAEYTGAYVFFATRGDTFPTTGALLNHDGGMGVRGFFEATGGKDLPQKLRLS

http://purl.uniprot.org/uniprot/P50206#attribution-609619A7DF3E53CE5E48EDEF8B8F9DD1
manual: true
evidence: ECO_0000250

http://purl.uniprot.org/uniprot/P50206#attribution-71E089CAE4A445B16847142C8E7D2A2D
manual: true
evidence: ECO_0000255
source: PRU10001

http://purl.uniprot.org/uniprot/P50206#attribution-080ED3982FEC43F7C43A0689717F3D20
manual: true
evidence: ECO_0000305

P17297
type: Protein
reviewed: true
created: 1990-08-01
modified: 2023-05-03
version: 117
mnemonic: BPHC_PSES1
old mnemonic: BPHC_PSESP
replaces: Q52441
citation: http://purl.uniprot.org/citations/2540155
citation: http://purl.uniprot.org/citations/8048958
citation: http://purl.uniprot.org/citations/8636975
citation: http://purl.uniprot.org/citations/11293547
citation: http://purl.uniprot.org/citations/12206778
recommended name: SIP71D827B41363451F
alternative name: SIP82DDA4E41BF35265
alternative name: SIP42943C32D384CDA0
enzyme: http://purl.uniprot.org/enzyme/1.13.11.39
organism: http://purl.uniprot.org/taxonomy/307
annotation: SIP4C01CD3067F28F43
annotation: SIP44DE6E27C604F3C0
annotation: SIPA2A011D1315505F2
annotation: SIP37888890934B62BA
annotation: SIPEACD4BF77725A69C
annotation: SIPD982965DDE65EF9B
annotation: PRO_0000085035
annotation: SIPB8B251CFF7781ADD
annotation: SIPCEEAEFF4C862277B
annotation: SIP62E56634E65711A3
annotation: SIP23F664AD50DB553F
annotation: SIPA41099D39791EBFC
annotation: SIPCAF30E78E9294106
annotation: SIPC0E28F2EFEEE8AB6
annotation: SIP9037F526D86BA860
annotation: SIPB84CCC9583E3DE37
annotation: SIP3A80E32663117B63
annotation: SIP8A9D8E212ABB06E8
annotation: SIPDE70A6757ED17E41
annotation: SIP4D3B13AF750B0978
annotation: SIP025A1DF7F71400C5
annotation: SIP738FDFA608D24B2B
annotation: SIP6C701C3C191877EB
annotation: SIPA881074BF2A2A9D6
annotation: SIP9D79EF8629ED5343
annotation: SIPD60420294A121A55
annotation: SIP8DB59AFF4BEBBDA7
annotation: SIP10992BAB4E0DDF87
annotation: SIPAC63ACD1BBE786F3
annotation: SIP9F4B85A36FEBC8F9
annotation: SIPBD54CE11515BDDB9
annotation: SIP55B1CE0DDCD3E15B
annotation: SIPD73053F79A56CCF9
annotation: SIPC2173FE5C21CBE3D
annotation: SIP42595BA7DC04C308
annotation: SIPC15F53AA8E91CACF
annotation: SIP5041678A22D501C6
annotation: SIPE5AA963D4D6CEAD4
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/2
classified with: http://purl.uniprot.org/keywords/58
classified with: http://purl.uniprot.org/keywords/223
classified with: http://purl.uniprot.org/keywords/408
classified with: http://purl.uniprot.org/keywords/479
classified with: http://purl.uniprot.org/keywords/677
classified with: GO_0018583
classified with: GO_0008198
classified with: GO_0019439
classified with: GO_0042178
sequence: P17297-1

P17297-citation-2540155
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

P17297-citation-8048958
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-16

P17297-citation-8636975
type: Citation Statement
scope: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS)

P17297-citation-11293547
type: Citation Statement
scope: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS)

P17297-citation-12206778
type: Citation Statement
scope: X-RAY CRYSTALLOGRAPHY (1.45 ANGSTROMS) OF NATIVE ENZYME; SUBSTRATE COMPLEXES AND H145A MUTANT

A32312
type: Resource
database: PIR
comment: DAPSPC

1DHY
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.30
chain sequence mapping: PDB 1DHY tt2tt293

PDB 1DHY tt2tt293
chain: A=2-293

1EIL
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.00
chain sequence mapping: PDB 1EIL tt2tt293

PDB 1EIL tt2tt293
chain: A=2-293

1EIQ
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.00
chain sequence mapping: PDB 1EIQ tt2tt293

PDB 1EIQ tt2tt293
chain: A=2-293

1EIR
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.00
chain sequence mapping: PDB 1EIR tt2tt293

PDB 1EIR tt2tt293
chain: A=2-293

1KW3
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 1.45
chain sequence mapping: PDB 1KW3 tt2tt293

PDB 1KW3 tt2tt293
chain: B=2-293

1KW6
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 1.45
chain sequence mapping: PDB 1KW6 tt2tt293

PDB 1KW6 tt2tt293
chain: B=2-293

1KW8
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.00
chain sequence mapping: PDB 1KW8 tt2tt293

PDB 1KW8 tt2tt293
chain: B=2-293

1KW9
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 1.95
chain sequence mapping: PDB 1KW9 tt2tt293

PDB 1KW9 tt2tt293
chain: B=2-293

1KWB
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.00
chain sequence mapping: PDB 1KWB tt2tt293

PDB 1KWB tt2tt293
chain: B=2-293

1KWC
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.10
chain sequence mapping: PDB 1KWC tt2tt293

PDB 1KWC tt2tt293
chain: B=2-293

DB02923
type: Resource
database: DrugBank
comment: Biphenyl-2,3-Diol

cd07237
type: Resource
database: CDD
comment: BphC1-RGP6_C_like
signature sequence match: CDD cd07237 match 1

cd07252
type: Resource
database: CDD
comment: BphC1-RGP6_N_like
signature sequence match: CDD cd07252 match 1

http://purl.uniprot.org/gene3d/3.10.180.10
type: Resource
database: Gene3D
comment: 2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1
signature sequence match: Gene3D 3.10.180.10 match 1
signature sequence match: Gene3D 3.10.180.10 match 2

IPR017626
type: Resource
database: InterPro
comment: DiOHbiphenyl_dOase

IPR029068
type: Resource
database: InterPro
comment: Glyas_Bleomycin-R_OHBP_Dase

IPR004360
type: Resource
database: InterPro
comment: Glyas_Fos-R_dOase_dom

IPR037523
type: Resource
database: InterPro
comment: VOC

IPR000486
type: Resource
database: InterPro
comment: Xdiol_ring_cleave_dOase_1/2

PF00903
type: Resource
database: Pfam
comment: Glyoxalase
signature sequence match: Pfam PF00903 match 1

SSF54593
type: Resource
database: SUPFAM
comment: Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase
signature sequence match: SUPFAM SSF54593 match 1
signature sequence match: SUPFAM SSF54593 match 2

TIGR03213
type: Resource
database: TIGRFAMs
comment: 23dbph12diox
signature sequence match: TIGRFAMs TIGR03213 match 1

PS00082
type: Resource
database: PROSITE
comment: EXTRADIOL_DIOXYGENAS
signature sequence match: PROSITE PS00082 match 1

PS51819
type: Resource
database: PROSITE
comment: VOC
signature sequence match: PROSITE PS51819 match 1
signature sequence match: PROSITE PS51819 match 2

SIP71D827B41363451F
type: Structured Name
full name: Biphenyl-2,3-diol 1,2-dioxygenase
ec name: 1.13.11.39

SIP82DDA4E41BF35265
type: Structured Name
full name: 2,3-dihydroxybiphenyl dioxygenase
short name: DHBD

SIP42943C32D384CDA0
type: Structured Name
full name: 23OHBP oxygenase

bphC
type: Gene

SIP37888890934B62BA
type: Subunit Annotation
comment: Homooctamer.

SIPEACD4BF77725A69C
type: Similarity Annotation
comment: Belongs to the extradiol ring-cleavage dioxygenase family.

SIPD982965DDE65EF9B
type: Initiator Methionine Annotation
comment: Removed
range: 22572111272425262tt1tt1

22572111272425262tt1
type: Position
type: ExactPosition
position: 1
reference: P17297-1

PRO_0000085035
type: Chain Annotation
comment: Biphenyl-2,3-diol 1,2-dioxygenase
mass: 32114
range: 22572111272425262tt2tt293

22572111272425262tt2
type: Position
type: ExactPosition
position: 2
reference: P17297-1

22572111272425262tt293
type: Position
type: ExactPosition
position: 293
reference: P17297-1

SIPB8B251CFF7781ADD
type: Domain Extent Annotation
comment: VOC 1
range: 22572111272425262tt5tt119

22572111272425262tt5
type: Position
type: ExactPosition
position: 5
reference: P17297-1

22572111272425262tt119
type: Position
type: ExactPosition
position: 119
reference: P17297-1

SIPCEEAEFF4C862277B
type: Domain Extent Annotation
comment: VOC 2
range: 22572111272425262tt143tt265

22572111272425262tt143
type: Position
type: ExactPosition
position: 143
reference: P17297-1

22572111272425262tt265
type: Position
type: ExactPosition
position: 265
reference: P17297-1

22572111272425262tt146
type: Position
type: ExactPosition
position: 146
reference: P17297-1

22572111272425262tt210
type: Position
type: ExactPosition
position: 210
reference: P17297-1

22572111272425262tt261
type: Position
type: ExactPosition
position: 261
reference: P17297-1

22572111272425262tt14
type: Position
type: ExactPosition
position: 14
reference: P17297-1

22572111272425262tt16
type: Position
type: ExactPosition
position: 16
reference: P17297-1

22572111272425262tt25
type: Position
type: ExactPosition
position: 25
reference: P17297-1

22572111272425262tt30
type: Position
type: ExactPosition
position: 30
reference: P17297-1

22572111272425262tt35
type: Position
type: ExactPosition
position: 35
reference: P17297-1

22572111272425262tt38
type: Position
type: ExactPosition
position: 38
reference: P17297-1

22572111272425262tt47
type: Position
type: ExactPosition
position: 47
reference: P17297-1

22572111272425262tt49
type: Position
type: ExactPosition
position: 49
reference: P17297-1

22572111272425262tt54
type: Position
type: ExactPosition
position: 54
reference: P17297-1

22572111272425262tt59
type: Position
type: ExactPosition
position: 59
reference: P17297-1

22572111272425262tt66
type: Position
type: ExactPosition
position: 66
reference: P17297-1

22572111272425262tt70
type: Position
type: ExactPosition
position: 70
reference: P17297-1

22572111272425262tt83
type: Position
type: ExactPosition
position: 83
reference: P17297-1

22572111272425262tt87
type: Position
type: ExactPosition
position: 87
reference: P17297-1

22572111272425262tt89
type: Position
type: ExactPosition
position: 89
reference: P17297-1

22572111272425262tt92
type: Position
type: ExactPosition
position: 92
reference: P17297-1

22572111272425262tt98
type: Position
type: ExactPosition
position: 98
reference: P17297-1

22572111272425262tt101
type: Position
type: ExactPosition
position: 101
reference: P17297-1

22572111272425262tt107
type: Position
type: ExactPosition
position: 107
reference: P17297-1

22572111272425262tt109
type: Position
type: ExactPosition
position: 109
reference: P17297-1

22572111272425262tt111
type: Position
type: ExactPosition
position: 111
reference: P17297-1

22572111272425262tt113
type: Position
type: ExactPosition
position: 113
reference: P17297-1

22572111272425262tt118
type: Position
type: ExactPosition
position: 118
reference: P17297-1

22572111272425262tt131
type: Position
type: ExactPosition
position: 131
reference: P17297-1

22572111272425262tt133
type: Position
type: ExactPosition
position: 133
reference: P17297-1

22572111272425262tt140
type: Position
type: ExactPosition
position: 140
reference: P17297-1

22572111272425262tt142
type: Position
type: ExactPosition
position: 142
reference: P17297-1

22572111272425262tt150
type: Position
type: ExactPosition
position: 150
reference: P17297-1

22572111272425262tt154
type: Position
type: ExactPosition
position: 154
reference: P17297-1

22572111272425262tt163
type: Position
type: ExactPosition
position: 163
reference: P17297-1

22572111272425262tt168
type: Position
type: ExactPosition
position: 168
reference: P17297-1

22572111272425262tt178
type: Position
type: ExactPosition
position: 178
reference: P17297-1

22572111272425262tt181
type: Position
type: ExactPosition
position: 181
reference: P17297-1

22572111272425262tt194
type: Position
type: ExactPosition
position: 194
reference: P17297-1

22572111272425262tt196
type: Position
type: ExactPosition
position: 196
reference: P17297-1

22572111272425262tt200
type: Position
type: ExactPosition
position: 200
reference: P17297-1

22572111272425262tt205
type: Position
type: ExactPosition
position: 205
reference: P17297-1

22572111272425262tt217
type: Position
type: ExactPosition
position: 217
reference: P17297-1

22572111272425262tt218
type: Position
type: ExactPosition
position: 218
reference: P17297-1

22572111272425262tt230
type: Position
type: ExactPosition
position: 230
reference: P17297-1

22572111272425262tt238
type: Position
type: ExactPosition
position: 238
reference: P17297-1

22572111272425262tt244
type: Position
type: ExactPosition
position: 244
reference: P17297-1

22572111272425262tt247
type: Position
type: ExactPosition
position: 247
reference: P17297-1

22572111272425262tt252
type: Position
type: ExactPosition
position: 252
reference: P17297-1

22572111272425262tt259
type: Position
type: ExactPosition
position: 259
reference: P17297-1

22572111272425262tt264
type: Position
type: ExactPosition
position: 264
reference: P17297-1

22572111272425262tt277
type: Position
type: ExactPosition
position: 277
reference: P17297-1

22572111272425262tt279
type: Position
type: ExactPosition
position: 279
reference: P17297-1

22572111272425262tt281
type: Position
type: ExactPosition
position: 281
reference: P17297-1

22572111272425262tt285
type: Position
type: ExactPosition
position: 285
reference: P17297-1

P17297-1
type: Simple Sequence
modified: 2007-01-23
version: 3
mass: 32245
value: MSIERLGYLGFAVKDVPAWDHFLTKSVGLMAAGSAGDAALYRADQRAWRIAVQPGELDDLAYAGLEVDDAAALERMADKLRQAGVAFTRGDEALMQQRKVMGLLCLQDPFGLPLEIYYGPAEIFHEPFLPSAPVSGFVTGDQGIGHFVRCVPDTAKAMAFYTEVLGFVLSDIIDIQMGPETSVPAHFLHCNGRHHTIALAAFPIPKRIHHFMLQANTIDDVGYAFDRLDAAGRITSLLGRHTNDQTLSFYADTPSPMIEVEFGWGPRTVDSSWTVARHSRTAMWGHKSVRGQR

http://purl.uniprot.org/uniprot/P17297#attribution-05C16B64E576893F0FB5E6F0F0FB0983
manual: true
evidence: ECO_0000255
source: PRU01163

http://purl.uniprot.org/uniprot/P17297#attribution-080ED3982FEC43F7C43A0689717F3D20
manual: true
evidence: ECO_0000305

P17548
type: Protein
reviewed: true
created: 1990-08-01
modified: 2023-05-03
version: 81
mnemonic: BPHD_PSES1
old mnemonic: BPHD_PSESP
citation: http://purl.uniprot.org/citations/2540155
conflicting sequence: SIP6563484F39D4EAD0
recommended name: SIP626AB1CCD6EEEFD1
alternative name: SIPDA13AB3CD1959653
enzyme: http://purl.uniprot.org/enzyme/3.7.1.8
organism: http://purl.uniprot.org/taxonomy/307
annotation: SIP88AF1266A9F24A12
annotation: SIPE3E69450BAF1B28B
annotation: SIP6BE3C4C3F9858781
annotation: SIP59B5AB953DFB5240
annotation: SIP78B8FB0402779CF9
annotation: SIP9F2E2989493AA5CE
annotation: PRO_0000207053
annotation: SIPAAD9654A0CE86AD8
annotation: SIP5156ED7AA8D498FB
annotation: SIP5A0A647EB15D2D4C
annotation: SIP5A484653235C4C82
annotation: SIP4310D1022D34034C
annotation: SIPF6FA32A87B3F359C
annotation: SIPC147F76E28AE2E43
annotation: SIPA5AE0BB1B11B996B
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/58
classified with: http://purl.uniprot.org/keywords/903
classified with: http://purl.uniprot.org/keywords/378
classified with: GO_0018774
classified with: GO_0018771
classified with: GO_0019439
sequence: P17548-1

P17548-citation-2540155
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
scope: PROTEIN SEQUENCE OF 2-33
scope: FUNCTION

EMBL AAA25751.1
comment: Frameshift.

B32312
type: Resource
database: PIR
comment: ESPSSK

psesl-bphD
type: Resource
database: ESTHER
comment: Carbon-carbon_bond_hydrolase

http://purl.uniprot.org/gene3d/3.40.50.1820
type: Resource
database: Gene3D
comment: alpha/beta hydrolase
signature sequence match: Gene3D 3.40.50.1820 match 1

MF_01688
type: Resource
database: HAMAP
comment: Biphenyl_BphD
signature sequence match: HAMAP MF 01688 match 1

IPR029058
type: Resource
database: InterPro
comment: AB_hydrolase

IPR000073
type: Resource
database: InterPro
comment: AB_hydrolase_1

IPR000639
type: Resource
database: InterPro
comment: Epox_hydrolase-like

IPR017727
type: Resource
database: InterPro
comment: HOPD_hydrolase_BphD

PTHR43689:SF8
type: Resource
database: PANTHER
comment: ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN
signature sequence match: PANTHER PTHR43689:SF8 match 1

PTHR43689
type: Resource
database: PANTHER
comment: HYDROLASE
signature sequence match: PANTHER PTHR43689 match 1

PF00561
type: Resource
database: Pfam
comment: Abhydrolase_1
signature sequence match: Pfam PF00561 match 1

PR00111
type: Resource
database: PRINTS
comment: ABHYDROLASE

PR00412
type: Resource
database: PRINTS
comment: EPOXHYDRLASE

SSF53474
type: Resource
database: SUPFAM
comment: alpha/beta-Hydrolases
signature sequence match: SUPFAM SSF53474 match 1

TIGR03343
type: Resource
database: TIGRFAMs
comment: biphenyl_bphD
signature sequence match: TIGRFAMs TIGR03343 match 1

SIP626AB1CCD6EEEFD1
type: Structured Name
full name: 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase
short name: HOPDA hydrolase
ec name: 3.7.1.8

SIPDA13AB3CD1959653
type: Structured Name
full name: 2,6-dioxo-6-phenylhexa-3-enoate hydrolase

bphD
type: Gene

SIP88AF1266A9F24A12
type: Function Annotation
comment: Catalyzes an unusual C-C bond hydrolysis of 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid (HOPDA) to produce benzoic acid and 2-hydroxy-2,4-pentadienoic acid (HPD).

SIP59B5AB953DFB5240
type: Subunit Annotation
comment: Homodimer.

SIP78B8FB0402779CF9
type: Similarity Annotation
comment: Belongs to the AB hydrolase superfamily. BphD family.

PRO_0000207053
type: Chain Annotation
comment: 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase
mass: 31589
range: 22572111322429486tt1tt286

22572111322429486tt1
type: Position
type: ExactPosition
position: 1
reference: P17548-1

22572111322429486tt286
type: Position
type: ExactPosition
position: 286
reference: P17548-1

SIPAAD9654A0CE86AD8
type: Active Site Annotation
comment: Proton acceptor
range: 22572111322429486tt265tt265

22572111322429486tt265
type: Position
type: ExactPosition
position: 265
reference: P17548-1

22572111322429486tt42
type: Position
type: ExactPosition
position: 42
reference: P17548-1

22572111322429486tt43
type: Position
type: ExactPosition
position: 43
reference: P17548-1

22572111322429486tt51
type: Position
type: ExactPosition
position: 51
reference: P17548-1

22572111322429486tt111
type: Position
type: ExactPosition
position: 111
reference: P17548-1

22572111322429486tt180
type: Position
type: ExactPosition
position: 180
reference: P17548-1

22572111322429486tt190
type: Position
type: ExactPosition
position: 190
reference: P17548-1

22572111322429486tt266
type: Position
type: ExactPosition
position: 266
reference: P17548-1

SIPA5AE0BB1B11B996B
type: Site Annotation
comment: Transition state stabilizer
range: 22572111322429486tt112tt112

22572111322429486tt112
type: Position
type: ExactPosition
position: 112
reference: P17548-1

substrate

P17548-1
type: Simple Sequence
modified: 2009-05-05
version: 2
mass: 31589
value: MSELNESSTSKFVTINEKGLSNFRIHLNDAGQGERVIMLHGGGPGAGGWSNYYRNIGPFVEAGYRVLLPDAPGFNKSDTVVMDEQRGLVNARSVKGMMDVLGIEKAHLVGNSMGGAGALNFALEYPERTGKLILMGPGGLGNSLFTAMPMEGIKLLFKLYAEPSLETLKQMLNVFLFDQSVITDELLQGRWANIQRNPEHLKNFILSAQKVPLSAWDVSARLGEIKAKTLVTWGRDDRFVPLDHGLKLIANMQDAHVHVFPRCAHWAQWEHADAFNRLTLDFLANG

http://purl.uniprot.org/uniprot/P17548#attribution-0EFB0BE05ED7E465A7A4A1E854B43307
manual: true
evidence: ECO_0000255
source: MF_01688

http://purl.uniprot.org/uniprot/P17548#attribution-BB9CD8943AB394A5D8BF28C170F76AB5
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/2540155

http://purl.uniprot.org/uniprot/P17548#attribution-080ED3982FEC43F7C43A0689717F3D20
manual: true
evidence: ECO_0000305

P58329
type: Protein
reviewed: true
created: 2001-10-24
modified: 2023-05-03
version: 64
mnemonic: CAH_PSESD
old mnemonic: ATZD_PSESD
replaces: G8HMW5
citation: http://purl.uniprot.org/citations/11544232
citation: http://purl.uniprot.org/citations/21959051
citation: SIP895B4FC14F3DB746
citation: http://purl.uniprot.org/citations/12788776
citation: http://purl.uniprot.org/citations/22730121
citation: http://purl.uniprot.org/citations/23651355
recommended name: SIPEDE374A95030F9B6
enzyme: http://purl.uniprot.org/enzyme/3.5.2.15
organism: http://purl.uniprot.org/taxonomy/47660
encoded in: pADP-1
annotation: SIP86DAD05AF133CB27
annotation: SIP94F74306971511DD
annotation: SIP08050515BC42D8E6
annotation: SIP2E5BF57821902E81
annotation: SIPB89262C686BFA265
annotation: SIP40773FFD95A33149
annotation: SIPF346076C4131DC27
annotation: SIP7C8B48CB26E1B56E
annotation: SIPAE3DA4E27807A1D1
annotation: PRO_0000064761
annotation: SIP73DB28C2C6755D05
annotation: SIPE38022D786933469
annotation: SIPC9C84D294AB49095
annotation: SIP866055E22BA5897C
annotation: SIPAFDED9554771AC59
annotation: SIP15EAC725B262DF03
annotation: SIPF1741A342233F7B4
annotation: SIPFFACF2803B7AEB5B
annotation: SIPDA75F7E54C394D35
annotation: SIPAC736BA209D1E8BD
annotation: SIPB6FE6B2D3133D44C
annotation: SIP2ECD2A9F0C0C1887
annotation: SIP64698905A89B203E
annotation: SIP440B59465BAB01A9
annotation: SIP7E43EAC1909F921B
annotation: SIP368A465EDA164C6A
annotation: SIP835BA78B87D49F2F
annotation: SIPDDBE36CA92923B0B
annotation: SIP12CB7A8D3F3D78D2
annotation: SIP40FF820A493BC1AC
annotation: SIPA80F80CB54E474B4
annotation: SIP5D2A06E58304EE44
annotation: SIP9E6569D9312921CF
annotation: SIP91A5D48E847D71F3
annotation: SIP1B76F31271229695
annotation: SIPDD5125F6AB4E41D8
annotation: SIPF5EA33B753993D80
annotation: SIPAAF91AA117AA6BDB
annotation: SIP53B1A113DFAA3561
annotation: SIPAC3F7863222B1860
annotation: SIPE57A610CA02579DD
annotation: SIP5CDAB54A04266D03
annotation: SIPD9446B39035C3F20
annotation: SIPC98F8A4D2087C1F5
annotation: SIP966F74E37C73A03B
annotation: SIP86D0C7D601D8F972
annotation: SIP42BC3AF07D7A60CE
annotation: SIP8457E7A452021B61
annotation: SIP65E67659920473DC
annotation: SIPA473B47A34CDD3C8
annotation: SIP60B299255EC78416
annotation: SIPB27946734A232C74
annotation: SIP787442810C810A9C
annotation: SIP3177F200DFB83496
annotation: SIP1A00321AD8A96B2B
annotation: SIP85C53E652CCA81AB
annotation: SIP9C0EB1A28177E268
annotation: SIP9664C046AF72370E
annotation: SIP6FDAABC06C20911D
annotation: SIP849DA8B7C63718DE
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/2

P58329-citation-11544232
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
scope: FUNCTION
scope: CATALYTIC ACTIVITY
scope: PATHWAY

ADP
type: Strain

P58329-citation-21959051
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

ADPE
type: Strain

SIP895B4FC14F3DB746
type: Submission Citation
title: Draft genome of the atrazine degrading bacteria Pseudomonas sp. ADP.
author: Devers-Lamrani M.
author: Spor A.
author: Mounier A.
author: Martin-Laurent F.
date: 2015-09
submitted to: EMBL/GenBank/DDBJ

P58329-citation-SIP895B4FC14F3DB746
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]

P58329-citation-12788776
type: Citation Statement
scope: FUNCTION
scope: BIOPHYSICOCHEMICAL PROPERTIES
scope: ACTIVITY REGULATION

P58329-citation-22730121
type: Citation Statement
scope: BIOPHYSICOCHEMICAL PROPERTIES

P58329-citation-23651355
type: Citation Statement
scope: X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) IN COMPLEX WITH METAL ION; SUBSTRATE AND INHIBITOR
scope: BIOPHYSICOCHEMICAL PROPERTIES
scope: SUBUNIT

4BVQ
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 1.90
chain sequence mapping: PDB 4BVQ tt1tt363

PDB 4BVQ tt1tt363
chain: A/B=1-363

4BVR
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.58
chain sequence mapping: PDB 4BVR tt1tt363

PDB 4BVR tt1tt363
chain: A/B=1-363

4BVS
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.60
chain sequence mapping: PDB 4BVS tt1tt363

PDB 4BVS tt1tt363
chain: A/B=1-363

4BVT
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 3.10
chain sequence mapping: PDB 4BVT tt1tt363

PDB 4BVT tt1tt363
chain: A/B=1-363

http://purl.uniprot.org/brenda/3.5.2.15
type: Resource
database: BRENDA
comment: 5085

http://purl.uniprot.org/gene3d/3.30.1330.160
type: Resource
database: Gene3D
comment: Cyanuric acid hydrolase/Barbituras, RU C
signature sequence match: Gene3D 3.30.1330.160 match 1

http://purl.uniprot.org/gene3d/3.30.1330.170
type: Resource
database: Gene3D
comment: Cyanuric acid hydrolase/Barbiturase, RU A
signature sequence match: Gene3D 3.30.1330.170 match 1

http://purl.uniprot.org/gene3d/3.30.1330.180
type: Resource
database: Gene3D
comment: Cyanuric acid hydrolase/Barbiturase, RU B
signature sequence match: Gene3D 3.30.1330.180 match 1

MF_01989
type: Resource
database: HAMAP
comment: Cyc_amidohydrol
signature sequence match: HAMAP MF 01989 match 1

IPR014086
type: Resource
database: InterPro
comment: AtzD/Barbiturase

IPR043008
type: Resource
database: InterPro
comment: AtzD/Barbiturase_RUA

IPR043006
type: Resource
database: InterPro
comment: AtzD/Barbiturase_RUB

IPR043007
type: Resource
database: InterPro
comment: AtzD/Barbiturase_RUC

PF09663
type: Resource
database: Pfam
comment: Amido_AtzD_TrzD
signature sequence match: Pfam PF09663 match 1

TIGR02714
type: Resource
database: TIGRFAMs
comment: amido_AtzD_TrzD
signature sequence match: TIGRFAMs TIGR02714 match 1

SIPEDE374A95030F9B6
type: Structured Name
full name: Cyanuric acid amidohydrolase
short name: CAH
ec name: 3.5.2.15

pADP-1
type: Plasmid

atzD
type: Gene
orf name: AOX63_31675

SIP86DAD05AF133CB27
type: Function Annotation
comment: Responsible for the hydrolysis of cyanuric acid, an intermediate formed during catabolism of s-triazine based compounds in herbicides such as atrazine and polymers such as melamine. Catalyzes the hydrolytic opening of the s-triazine ring of cyanuric acid (2,4,6-trihydroxy-s-triazine) to yield carbon dioxide and carboxybiuret, which spontaneously decarboxylates to biuret.

SIP08050515BC42D8E6
type: Activity Regulation Annotation
comment: Inhibited by barbituric acid.

SIP2E5BF57821902E81
type: Kinetics Annotation
comment: kcat is 73 sec(-1) with cyanuric acid as substrate (PubMed:22730121). kcat is 6.8 sec(-1) with cyanuric acid as substrate and 3.1 sec(-1) with N-methylisocyanuric acid as substrate (PubMed:12788776).
measured affinity: 23 uM for cyanuric acid
measured affinity: 57 uM for cyanuric acid
measured affinity: 71 uM for N-methylisocyanuric acid

SIPB89262C686BFA265
type: PH Dependence Annotation
comment: Optimum pH is 8.2.

SIPF346076C4131DC27
type: Subunit Annotation
comment: Homotetramer.

SIP7C8B48CB26E1B56E
type: Domain Annotation
comment: The monomer structure is formed from three repeating units (RUs) that share the same structure as one another. The monomer, the active site and substrate all possess threefold rotational symmetry, to the extent that the active site possesses three potential Ser-Lys catalytic dyads. It is possible that any or all of the three active-site serines may act as nucleophile (albeit only one can do so per catalytic cycle).

SIPAE3DA4E27807A1D1
type: Similarity Annotation
comment: Belongs to the cyclic amide hydrolase (CyAH) family.

PRO_0000064761
type: Chain Annotation
comment: Cyanuric acid amidohydrolase
mass: 38200
range: 22576513630222638tt1tt363

22576513630222638tt1
type: Position
type: ExactPosition
position: 1
reference: P58329-1

22576513630222638tt363
type: Position
type: ExactPosition
position: 363
reference: P58329-1

SIP73DB28C2C6755D05
type: Region Annotation
comment: RU A
range: 22576513630222638tt1tt104

22576513630222638tt104
type: Position
type: ExactPosition
position: 104
reference: P58329-1

SIPE38022D786933469
type: Region Annotation
comment: RU B
range: 22576513630222638tt112tt249

22576513630222638tt112
type: Position
type: ExactPosition
position: 112
reference: P58329-1

22576513630222638tt249
type: Position
type: ExactPosition
position: 249
reference: P58329-1

SIPC9C84D294AB49095
type: Region Annotation
comment: RU C
range: 22576513630222638tt255tt363

22576513630222638tt255
type: Position
type: ExactPosition
position: 255
reference: P58329-1

22576513630222638tt162
type: Position
type: ExactPosition
position: 162
reference: P58329-1

SIPAFDED9554771AC59
type: Active Site Annotation
comment: Nucleophile
range: 22576513630222638tt232tt232

22576513630222638tt232
type: Position
type: ExactPosition
position: 232
reference: P58329-1

22576513630222638tt52
type: Position
type: ExactPosition
position: 52
reference: P58329-1

22576513630222638tt83
type: Position
type: ExactPosition
position: 83
reference: P58329-1

22576513630222638tt84
type: Position
type: ExactPosition
position: 84
reference: P58329-1

22576513630222638tt194
type: Position
type: ExactPosition
position: 194
reference: P58329-1

22576513630222638tt233
type: Position
type: ExactPosition
position: 233
reference: P58329-1

22576513630222638tt297
type: Position
type: ExactPosition
position: 297
reference: P58329-1

22576513630222638tt324
type: Position
type: ExactPosition
position: 324
reference: P58329-1

22576513630222638tt343
type: Position
type: ExactPosition
position: 343
reference: P58329-1

22576513630222638tt344
type: Position
type: ExactPosition
position: 344
reference: P58329-1

22576513630222638tt346
type: Position
type: ExactPosition
position: 346
reference: P58329-1

22576513630222638tt349
type: Position
type: ExactPosition
position: 349
reference: P58329-1

22576513630222638tt350
type: Position
type: ExactPosition
position: 350
reference: P58329-1

22576513630222638tt351
type: Position
type: ExactPosition
position: 351
reference: P58329-1

22576513630222638tt354
type: Position
type: ExactPosition
position: 354
reference: P58329-1

SIPDDBE36CA92923B0B
type: Site Annotation
comment: Important for substrate specificity
range: 22576513630222638tt320tt320

22576513630222638tt320
type: Position
type: ExactPosition
position: 320
reference: P58329-1

22576513630222638tt2
type: Position
type: ExactPosition
position: 2
reference: P58329-1

22576513630222638tt10
type: Position
type: ExactPosition
position: 10
reference: P58329-1

22576513630222638tt18
type: Position
type: ExactPosition
position: 18
reference: P58329-1

22576513630222638tt25
type: Position
type: ExactPosition
position: 25
reference: P58329-1

22576513630222638tt31
type: Position
type: ExactPosition
position: 31
reference: P58329-1

22576513630222638tt33
type: Position
type: ExactPosition
position: 33
reference: P58329-1

22576513630222638tt34
type: Position
type: ExactPosition
position: 34
reference: P58329-1

22576513630222638tt43
type: Position
type: ExactPosition
position: 43
reference: P58329-1

22576513630222638tt51
type: Position
type: ExactPosition
position: 51
reference: P58329-1

22576513630222638tt67
type: Position
type: ExactPosition
position: 67
reference: P58329-1

22576513630222638tt71
type: Position
type: ExactPosition
position: 71
reference: P58329-1

22576513630222638tt77
type: Position
type: ExactPosition
position: 77
reference: P58329-1

22576513630222638tt78
type: Position
type: ExactPosition
position: 78
reference: P58329-1

22576513630222638tt93
type: Position
type: ExactPosition
position: 93
reference: P58329-1

22576513630222638tt101
type: Position
type: ExactPosition
position: 101
reference: P58329-1

22576513630222638tt119
type: Position
type: ExactPosition
position: 119
reference: P58329-1

22576513630222638tt125
type: Position
type: ExactPosition
position: 125
reference: P58329-1

22576513630222638tt127
type: Position
type: ExactPosition
position: 127
reference: P58329-1

22576513630222638tt131
type: Position
type: ExactPosition
position: 131
reference: P58329-1

22576513630222638tt148
type: Position
type: ExactPosition
position: 148
reference: P58329-1

22576513630222638tt153
type: Position
type: ExactPosition
position: 153
reference: P58329-1

22576513630222638tt155
type: Position
type: ExactPosition
position: 155
reference: P58329-1

22576513630222638tt156
type: Position
type: ExactPosition
position: 156
reference: P58329-1

22576513630222638tt163
type: Position
type: ExactPosition
position: 163
reference: P58329-1

22576513630222638tt168
type: Position
type: ExactPosition
position: 168
reference: P58329-1

22576513630222638tt176
type: Position
type: ExactPosition
position: 176
reference: P58329-1

22576513630222638tt186
type: Position
type: ExactPosition
position: 186
reference: P58329-1

22576513630222638tt204
type: Position
type: ExactPosition
position: 204
reference: P58329-1

22576513630222638tt210
type: Position
type: ExactPosition
position: 210
reference: P58329-1

22576513630222638tt212
type: Position
type: ExactPosition
position: 212
reference: P58329-1

22576513630222638tt215
type: Position
type: ExactPosition
position: 215
reference: P58329-1

22576513630222638tt217
type: Position
type: ExactPosition
position: 217
reference: P58329-1

22576513630222638tt218
type: Position
type: ExactPosition
position: 218
reference: P58329-1

22576513630222638tt220
type: Position
type: ExactPosition
position: 220
reference: P58329-1

22576513630222638tt226
type: Position
type: ExactPosition
position: 226
reference: P58329-1

22576513630222638tt241
type: Position
type: ExactPosition
position: 241
reference: P58329-1

22576513630222638tt248
type: Position
type: ExactPosition
position: 248
reference: P58329-1

22576513630222638tt254
type: Position
type: ExactPosition
position: 254
reference: P58329-1

22576513630222638tt264
type: Position
type: ExactPosition
position: 264
reference: P58329-1

22576513630222638tt268
type: Position
type: ExactPosition
position: 268
reference: P58329-1

22576513630222638tt277
type: Position
type: ExactPosition
position: 277
reference: P58329-1

22576513630222638tt283
type: Position
type: ExactPosition
position: 283
reference: P58329-1

22576513630222638tt288
type: Position
type: ExactPosition
position: 288
reference: P58329-1

22576513630222638tt289
type: Position
type: ExactPosition
position: 289
reference: P58329-1

22576513630222638tt296
type: Position
type: ExactPosition
position: 296
reference: P58329-1

22576513630222638tt302
type: Position
type: ExactPosition
position: 302
reference: P58329-1

22576513630222638tt304
type: Position
type: ExactPosition
position: 304
reference: P58329-1

22576513630222638tt311
type: Position
type: ExactPosition
position: 311
reference: P58329-1

22576513630222638tt313
type: Position
type: ExactPosition
position: 313
reference: P58329-1

22576513630222638tt315
type: Position
type: ExactPosition
position: 315
reference: P58329-1

22576513630222638tt317
type: Position
type: ExactPosition
position: 317
reference: P58329-1

22576513630222638tt319
type: Position
type: ExactPosition
position: 319
reference: P58329-1

22576513630222638tt335
type: Position
type: ExactPosition
position: 335
reference: P58329-1

22576513630222638tt336
type: Position
type: ExactPosition
position: 336
reference: P58329-1

22576513630222638tt338
type: Position
type: ExactPosition
position: 338
reference: P58329-1

22576513630222638tt340
type: Position
type: ExactPosition
position: 340
reference: P58329-1

22576513630222638tt355
type: Position
type: ExactPosition
position: 355
reference: P58329-1

22576513630222638tt362
type: Position
type: ExactPosition
position: 362
reference: P58329-1

substrate

SIP6F18390F7375B093
subclass of: CHEBI_18420
comment: structural

P58329-1
type: Simple Sequence
modified: 2001-10-24
version: 1
mass: 38200
value: MYHIDVFRIPCHSPGDTSGLEDLIETGRVAPADIVAVMGKTEGNGCVNDYTREYATAMLAACLGRHLQLPPHEVEKRVAFVMSGGTEGVLSPHHTVFARRPAIDAHRPAGKRLTLGIAFTRDFLPEEIGRHAQITETAGAVKRAMRDAGIASIDDLHFVQVKCPLLTPAKIASARSRGCAPVTTDTYESMGYSRGASALGIALATEEVPSSMLVDESVLNDWSLSSSLASASAGIELEHNVVIAIGMSEQATSELVIAHGVMSDAIDAASVRRTIESLGIRSDDEMDRIVNVFAKAEASPDGVVRGMRHTMLSDSDINSTRHARAVTGAAIASVVGHGMVYVSGGAEHQGPAGGGPFAVIARA

http://purl.uniprot.org/uniprot/P58329#attribution-22B10FAD04D218CC42BE31252EEBB443
manual: true
evidence: ECO_0000255
source: MF_01989

http://purl.uniprot.org/uniprot/P58329#attribution-C9EF48738FB9398C0F636AF39D52C894
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/11544232

http://purl.uniprot.org/uniprot/P58329#attribution-EE92DE42767327A3FBBCB724841C09BB
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/12788776

http://purl.uniprot.org/uniprot/P58329#attribution-6633777B51B7BBEA108759F4BB61836E
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/22730121

http://purl.uniprot.org/uniprot/P58329#attribution-BF6D48A5943A574F1A8DC966E9F0AE0A
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/23651355

http://purl.uniprot.org/uniprot/P58329#attribution-44E0A8E9DD77D1ED3901EC0A6FD54E00
manual: true
evidence: ECO_0000305

http://purl.uniprot.org/uniprot/P58329#attribution-23C4BB480A7D1A6DE17A60B07F9447DF
manual: true
evidence: ECO_0000305
source: http://purl.uniprot.org/citations/23651355

A5JTM6
type: Protein
reviewed: true
created: 2010-11-30
modified: 2023-05-03
version: 44
mnemonic: CBACL_PSEUC
citation: http://purl.uniprot.org/citations/1351742
citation: SIP1B3FB6BEB7CEBA2C
citation: http://purl.uniprot.org/citations/1418673
citation: http://purl.uniprot.org/citations/1610806
citation: http://purl.uniprot.org/citations/9398293
recommended name: SIPCEBE075D2BC4DB72
enzyme: http://purl.uniprot.org/enzyme/6.2.1.33
organism: http://purl.uniprot.org/taxonomy/72586
annotation: SIPFC81CD7FA98C77F7
annotation: SIP33B1984604FDD6EC
annotation: SIP127AADDBDCD6661E
annotation: SIPB5CFF32C8E8ECE24
annotation: SIP9184E5FF7F22882B
annotation: SIPB4AA2028808A396D
annotation: SIP5491808D96A1B8F6
annotation: SIP2FB97639E52C8F7A
annotation: SIPC6441625F3912602
annotation: SIP303629B1C1FA129E
annotation: PRO_0000401169
annotation: SIP51CD200FFF629255
annotation: SIPB6E13D55301BB1FD
annotation: SIP3256A3C0AD3D10C0
annotation: SIPC3B24FA3238E3BAB
annotation: SIP678C914FD30B3D9D
annotation: SIP9D8683BD357FD0F5
annotation: SIP6796498505A936BE
annotation: SIPA272E6E200A8332D
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/67
classified with: http://purl.uniprot.org/keywords/903
classified with: http://purl.uniprot.org/keywords/436
classified with: GO_0018861
classified with: GO_0005524
sequence: A5JTM6-1

A5JTM6-citation-1351742
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

A5JTM6-citation-SIP1B3FB6BEB7CEBA2C
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

A5JTM6-citation-1418673
type: Citation Statement
scope: PROTEIN SEQUENCE OF 1-16
scope: FUNCTION
scope: CATALYTIC ACTIVITY
scope: COFACTOR
scope: ACTIVITY REGULATION
scope: BIOPHYSICOCHEMICAL PROPERTIES
scope: SUBUNIT

A5JTM6-citation-1610806
type: Citation Statement
scope: FUNCTION
scope: CATALYTIC ACTIVITY
scope: COFACTOR
scope: BIOPHYSICOCHEMICAL PROPERTIES
scope: PATHWAY
scope: SUBUNIT

A5JTM6-citation-9398293
type: Citation Statement
scope: FUNCTION
scope: CATALYTIC ACTIVITY
scope: BIOPHYSICOCHEMICAL PROPERTIES
scope: MUTAGENESIS OF GLY-163; GLY-166; PRO-168; LYS-169 AND GLU-306

cd05923
type: Resource
database: CDD
comment: CBAL
signature sequence match: CDD cd05923 match 1

IPR025110
type: Resource
database: InterPro
comment: AMP-bd_C

IPR045851
type: Resource
database: InterPro
comment: AMP-bd_C_sf

IPR020845
type: Resource
database: InterPro
comment: AMP-binding_CS

IPR000873
type: Resource
database: InterPro
comment: AMP-dep_Synth/Lig_com

PTHR43201
type: Resource
database: PANTHER
comment: ACYL-COA SYNTHETASE
signature sequence match: PANTHER PTHR43201 match 1

PTHR43201:SF8
type: Resource
database: PANTHER
comment: FATTY ACID COA SYNTHETASE FAMILY
signature sequence match: PANTHER PTHR43201:SF8 match 1

PF00501
type: Resource
database: Pfam
comment: AMP-binding
signature sequence match: Pfam PF00501 match 1

PF13193
type: Resource
database: Pfam
comment: AMP-binding_C
signature sequence match: Pfam PF13193 match 1

SSF56801
type: Resource
database: SUPFAM
comment: Acetyl-CoA synthetase-like
signature sequence match: SUPFAM SSF56801 match 1

PS00455
type: Resource
database: PROSITE
comment: AMP_BINDING
signature sequence match: PROSITE PS00455 match 1

SIPCEBE075D2BC4DB72
type: Structured Name
full name: 4-chlorobenzoate--CoA ligase
short name: 4-CBA:CoA ligase
ec name: 6.2.1.33

SIPFC81CD7FA98C77F7
type: Function Annotation
comment: Catalyzes the formation of chlorobenzoyl-CoA via a 2 step reaction. First 4-chlorobenzoyl is adenylated by ATP, followed by acyl transfer from the 4-chlorobenzoyl-AMP intermediate to CoA. Benzoate, 4-bromobenzoate, 4-iodobenzoate and 4-methylbenzoate also act as substrates. Inactive towards 4-aminobenzoate, 4-hydroxybenzoate, 2-aminobenzoate, 2,3-dihydroxybenzoate, 4-coumarate and the aliphatic carboxylic acids palmate, caproate, laurate and butyrate. Negligible activity is detected when ATP is replaced by UTP, CTP or GTP as cosubstrate.

SIPB5CFF32C8E8ECE24
type: Activity Regulation Annotation
comment: Unaffected by 5,5'-dithiobis-(2-nitrobenzoic acid), 4-chloromercuribenzoate and sodium azide. Inhibited by Cu(2+), Fe(2+) and Zn(2+). Unaffected by Na(+), K(+) and Li(+).

SIP9184E5FF7F22882B
type: Kinetics Annotation
measured affinity: 8.5 uM for 4-chlorobenzoate
measured affinity: 70 uM for CoA
measured affinity: 104 uM for ATP (with magnesium as cofactor)
measured affinity: 43 uM for ATP (with manganese as cofactor)
measured affinity: 59 uM for ATP (with cobalt as cofactor)
measured affinity: 15 uM for 4-bromobenzoate
measured affinity: 17 uM for 4-iodobenzoate
measured affinity: 700 uM for benzoate
measured affinity: 130 uM for 4-methylbenzoate

SIPB4AA2028808A396D
type: PH Dependence Annotation
comment: Optimum pH is 8.4. 85% of activity remains at pH 9.0, 54% of activity remains at pH 7.0.

SIP5491808D96A1B8F6
type: Temperature Dependence Annotation
comment: Optimum temperature is 35 degrees Celsius.

SIPC6441625F3912602
type: Subunit Annotation
comment: Homodimer.

SIP303629B1C1FA129E
type: Similarity Annotation
comment: Belongs to the ATP-dependent AMP-binding enzyme family.

PRO_0000401169
type: Chain Annotation
comment: 4-chlorobenzoate--CoA ligase
mass: 57151
range: 18354466844390958tt1tt528

18354466844390958tt1
type: Position
type: ExactPosition
position: 1
reference: A5JTM6-1

18354466844390958tt528
type: Position
type: ExactPosition
position: 528
reference: A5JTM6-1

18354466844390958tt161
type: Position
type: ExactPosition
position: 161
reference: A5JTM6-1

18354466844390958tt169
type: Position
type: ExactPosition
position: 169
reference: A5JTM6-1

18354466844390958tt300
type: Position
type: ExactPosition
position: 300
reference: A5JTM6-1

18354466844390958tt305
type: Position
type: ExactPosition
position: 305
reference: A5JTM6-1

18354466844390958tt409
type: Position
type: ExactPosition
position: 409
reference: A5JTM6-1

SIPC3B24FA3238E3BAB
type: Mutagenesis Annotation
comment: Significantly inhibits the adenylation part of the reaction.
substitution: I
range: 18354466844390958tt163tt163

18354466844390958tt163
type: Position
type: ExactPosition
position: 163
reference: A5JTM6-1

SIP678C914FD30B3D9D
type: Mutagenesis Annotation
comment: Significantly inhibits the adenylation part of the reaction.
substitution: I
range: 18354466844390958tt166tt166

18354466844390958tt166
type: Position
type: ExactPosition
position: 166
reference: A5JTM6-1

SIP9D8683BD357FD0F5
type: Mutagenesis Annotation
comment: No catalytic activity.
substitution: A
range: 18354466844390958tt168tt168

18354466844390958tt168
type: Position
type: ExactPosition
position: 168
reference: A5JTM6-1

SIP6796498505A936BE
type: Mutagenesis Annotation
comment: Significantly inhibits the adenylation part of the reaction.
substitution: M
range: 18354466844390958tt169tt169

SIPA272E6E200A8332D
type: Mutagenesis Annotation
comment: Significantly inhibits the adenylation part of the reaction.
substitution: Q
range: 18354466844390958tt306tt306

18354466844390958tt306
type: Position
type: ExactPosition
position: 306
reference: A5JTM6-1

A5JTM6-1
type: Simple Sequence
modified: 2007-06-26
version: 1
mass: 57151
value: MQTVHEMLRRAVSRVPHRWAIVDAARSTFDICRTGETSRNEGSATARLWPQPARPLAVVSGNSVEAVIAVLALHRLQAVPALMNPRLKPAEISELVARGEMARAVVANDAGVMEAIRTRVPSVCVLALDDLVSGSRVPEVAGKSLPPPPCEPEQAGFVFYTSGTTGLPKGAVIPQRAAESRVLFMATQAGLRHGSHNVVLGLMPLYHTIGFFAVLVAAMAFDGTYVVVEEFDAGNVLKLIERERVTAMFATPTHLDALTTAVEQAGARLESLEHVTFAGATMPDTVLERVNRFIPGEKVNIYGTTEAMNSLYMRAVRIAGTVMRPGFFSEVRIVRVGGDVDDGCPTVKRASWRWRRRMRPFQATLTNLRLLQKSFRKAGTGRAICVRDGSGNIVVLGRVDDMIISGGENIHPSEVERILAAAPGVAEVVVIGVKDERWGQSVVACVVLQPGASASAERLDAFCRASALADFKRPRRYVFLDELPKSAMNKVLRRQLMQHVSATSSAAVVPAPAVKQRTYAPSGRAIAR

http://purl.uniprot.org/uniprot/A5JTM6#attribution-A4F426C0AE6FF0054E952F05514A7399
creator: UniProtKB
manual: true
evidence: ECO_0000314
source: http://purl.uniprot.org/citations/1418673

http://purl.uniprot.org/uniprot/A5JTM6#attribution-609619A7DF3E53CE5E48EDEF8B8F9DD1
manual: true
evidence: ECO_0000250

http://purl.uniprot.org/uniprot/A5JTM6#attribution-63354F0DE400DC8EDCB7771F87402F6C
manual: true
evidence: ECO_0000255

http://purl.uniprot.org/uniprot/A5JTM6#attribution-0AD3424D481C7FC7123BC795FCA41ABF
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/1418673

http://purl.uniprot.org/uniprot/A5JTM6#attribution-52A33D937FBDF6FE83A720940977CC24
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/1610806

http://purl.uniprot.org/uniprot/A5JTM6#attribution-999E7165EE0C6C4F71BB4CD42EF0B2B1
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/9398293

http://purl.uniprot.org/uniprot/A5JTM6#attribution-6E0F5C938A81EDA1F76E95397D236654
manual: true
evidence: ECO_0000303
source: http://purl.uniprot.org/citations/1610806

http://purl.uniprot.org/uniprot/A5JTM6#attribution-B46D5B095E4E0B3BC450257B8FD9ED78
manual: true
evidence: ECO_0000303
source: http://purl.uniprot.org/citations/9398293

http://purl.uniprot.org/uniprot/A5JTM6#attribution-DE456E336AB38AC98E24008396E8DFDF
manual: true
evidence: ECO_0000305

http://purl.uniprot.org/uniprot/A5JTM6#attribution-259347A2937235F99FCFBDB16155273F
manual: true
evidence: ECO_0000312
source: ABQ44579.1

A5JTM5
type: Protein
reviewed: true
created: 2010-11-30
modified: 2023-05-03
version: 62
mnemonic: CBADH_PSEUC
citation: http://purl.uniprot.org/citations/1351742
citation: SIP1B3FB6BEB7CEBA2C
citation: http://purl.uniprot.org/citations/7579994
citation: http://purl.uniprot.org/citations/1610806
citation: http://purl.uniprot.org/citations/8218302
citation: http://purl.uniprot.org/citations/8718880
citation: http://purl.uniprot.org/citations/9063883
citation: http://purl.uniprot.org/citations/11747444
citation: http://purl.uniprot.org/citations/12899635
citation: http://purl.uniprot.org/citations/16388585
citation: http://purl.uniprot.org/citations/8679561
citation: http://purl.uniprot.org/citations/11695894
recommended name: SIP6E422562F2191833
enzyme: http://purl.uniprot.org/enzyme/3.8.1.7
organism: http://purl.uniprot.org/taxonomy/72586
annotation: SIP13CC26A3F42300BA
annotation: SIPA7BE788A9E15E481
annotation: SIP7947C43E6F685378
annotation: SIP144C34EEA41E261C
annotation: SIP1ABB2AB519964882
annotation: SIP471147B6B6CF310F
annotation: SIPD5A25FD0081B438E
annotation: SIP8571FEB4BF148B66
annotation: SIP609DCB093442B320
annotation: SIP42DC221D75708BC0
annotation: PRO_0000401147
annotation: SIP116AE435C5011537
annotation: SIP4EEFB2C39BAB2969
annotation: SIP72250D18A1144401
annotation: SIP9960A69EAC48615F
annotation: SIPE7471EE58645A777
annotation: SIP64238AFE02999D81
annotation: SIP45C1479F2C34131B
annotation: SIP656DCB951EFC968E
annotation: SIP3441CE34937C24BE
annotation: SIP4BEA8E3DE3E3F94E
annotation: SIPEA4DB60636EB881A
annotation: SIP6FFB876436901148
annotation: SIP7D837730B37639E6
annotation: SIPB379360C4B51478D
annotation: SIPAC4B8EB7F6193159
annotation: SIP7FCF3FE724E07A19
annotation: SIPD33F937A70B5EF21
annotation: SIP61BC28D472736170
annotation: SIPDE3185066BF78A87
annotation: SIP365BD86C72979A75
annotation: SIP0E8CEF697B4DB8C8
annotation: SIP6C74DB5C8A2A9FE6
annotation: SIPE29CCDAFF9C5D68A
annotation: SIP2EF8208755A33776
annotation: SIP6E0E79F47FC5F05F
annotation: SIP94FC8F3FA108F600
annotation: SIP8C20DF1903DCDF34
annotation: SIP07E806EEEB999D3A
annotation: SIPB3D6F502CB08744D
annotation: SIPD1C431BF7CE54D0D
annotation: SIPE142112DD8EE0E2D
annotation: SIP50F0CD968E7AFAFC
annotation: SIPAA587BC6DBA4D7C9
annotation: SIP183E206360EAF090
annotation: SIPA2B724F14F4713BC
annotation: SIPD56CA2C388E5EDA9
annotation: SIPD9103B1547679BB1
annotation: SIP6ED74020467F6DEA
annotation: SIP5314A3BDE3FD6F67
annotation: SIP40D71AED75F0057D
annotation: SIP80207CF1BCB98B4D
annotation: SIP56AA8867394630F0
annotation: SIP7B1DE0A1E776A1F6
annotation: SIPD2D45012292FA539
annotation: SIP793699EEE1F0B1E8
annotation: SIP1AA9DCE5B2E2B0BD
annotation: SIP37D8C500FCCFCC74
annotation: SIP493AE5B3EEF82348

A5JTM5-citation-1351742
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

A5JTM5-citation-SIP1B3FB6BEB7CEBA2C
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

CBS-3
type: Strain

A5JTM5-citation-7579994
type: Citation Statement
scope: PROTEIN SEQUENCE OF 1-40
scope: FUNCTION
scope: CATALYTIC ACTIVITY
scope: ACTIVITY REGULATION
scope: BIOPHYSICOCHEMICAL PROPERTIES
scope: SUBUNIT
scope: INDUCTION

A5JTM5-citation-1610806
type: Citation Statement
scope: FUNCTION
scope: CATALYTIC ACTIVITY
scope: ACTIVITY REGULATION
scope: BIOPHYSICOCHEMICAL PROPERTIES
scope: PATHWAY
scope: SUBUNIT

A5JTM5-citation-8218302
type: Citation Statement
scope: BIOPHYSICOCHEMICAL PROPERTIES

A5JTM5-citation-8718880
type: Citation Statement
scope: ACTIVITY REGULATION
scope: ACTIVE SITES
scope: MUTAGENESIS OF GLU-34; GLU-43; ASP-45; ASP-46; GLU-68; HIS-90; HIS-94; ASP-123; ASP-129; TRP-137; GLU-163; GLU-175; TRP-179 AND HIS-208

A5JTM5-citation-9063883
type: Citation Statement
scope: BIOPHYSICOCHEMICAL PROPERTIES
scope: MUTAGENESIS OF PHE-64; HIS-81; PHE-82; TRP-89; HIS-90; GLY-114; TRP-137 AND ASP-145

A5JTM5-citation-11747444
type: Citation Statement
scope: BIOPHYSICOCHEMICAL PROPERTIES
scope: MUTAGENESIS OF ARG-24; PHE-64; ARG-67; GLY-113; GLY-114 AND ARG-257

A5JTM5-citation-12899635
type: Citation Statement
scope: BIOPHYSICOCHEMICAL PROPERTIES
scope: MUTAGENESIS OF GLY-63; PHE-64; TYR-65; ALA-112; GLY-113 AND GLY-115

A5JTM5-citation-16388585
type: Citation Statement
scope: BIOPHYSICOCHEMICAL PROPERTIES
scope: MUTAGENESIS OF ARG-216 AND GLU-232

A5JTM5-citation-8679561
type: Citation Statement
scope: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) OF MUTANT GLN-90 IN COMPLEX WITH 4-HYDROXYBENZOYL COENZYME A
scope: SUBUNIT
scope: ACTIVE SITE
scope: SUBSTRATE-BINDING SITES

A5JTM5-citation-11695894
type: Citation Statement
scope: X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) OF MUTANT GLN-90 IN COMPLEX WITH 4-HYDROXYBENZOYL COENZYME A
scope: BIOPHYSICOCHEMICAL PROPERTIES
scope: MUTAGENESIS OF HIS-90

1JXZ
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 1.90
chain sequence mapping: PDB 1JXZ tt1tt269

PDB 1JXZ tt1tt269
chain: A/B/C=1-269

1NZY
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 1.80
chain sequence mapping: PDB 1NZY tt1tt269

PDB 1NZY tt1tt269
chain: A/B/C=1-269

http://purl.uniprot.org/brenda/3.8.1.7
type: Resource
database: BRENDA
comment: 5085

cd06558
type: Resource
database: CDD
comment: crotonase-like
signature sequence match: CDD cd06558 match 1

http://purl.uniprot.org/gene3d/1.10.12.10
type: Resource
database: Gene3D
comment: Lyase 2-enoyl-coa Hydratase, Chain A, domain 2
signature sequence match: Gene3D 1.10.12.10 match 1

IPR029045
type: Resource
database: InterPro
comment: ClpP/crotonase-like_dom_sf

IPR018376
type: Resource
database: InterPro
comment: Enoyl-CoA_hyd/isom_CS

IPR001753
type: Resource
database: InterPro
comment: Enoyl-CoA_hydra/iso

IPR014748
type: Resource
database: InterPro
comment: Enoyl-CoA_hydra_C

PTHR43684:SF4
type: Resource
database: PANTHER
comment: ENOYL-COA HYDRATASE/ISOMERASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_1G01890)
signature sequence match: PANTHER PTHR43684:SF4 match 1

PF00378
type: Resource
database: Pfam
comment: ECH_1
signature sequence match: Pfam PF00378 match 1

SSF52096
type: Resource
database: SUPFAM
comment: ClpP/crotonase
signature sequence match: SUPFAM SSF52096 match 1

PS00166
type: Resource
database: PROSITE
comment: ENOYL_COA_HYDRATASE
signature sequence match: PROSITE PS00166 match 1

SIP6E422562F2191833
type: Structured Name
full name: 4-chlorobenzoyl coenzyme A dehalogenase
short name: 4-CBA-CoA dehalogenase
short name: 4-CBCoA dehalogenase
short name: 4-chlorobenzoyl-CoA dehalogenase
ec name: 3.8.1.7

SIP13CC26A3F42300BA
type: Function Annotation
comment: Dehalogenates 4-chlorobenzoyl-CoA, 4-iodobenzoyl-CoA and 4-bromobenzoyl-CoA, but not 4-fluorobenzoyl-CoA. Inactive towards crotonyl-CoA, alpha-methylcrotonyl-CoA and beta-methylcrotonyl-CoA.

SIP7947C43E6F685378
type: Activity Regulation Annotation
comment: Inactivated by 1 mM Ag(+) and by 5 mM Cu(2+). Partially inhibited by 5 mM Zn(2+), Mn(2+), Co(2+), Fe(2+) and Ni(2+). Unaffected by 10 mM Na(+), K(+) and Li(+) and by 0.5 mM Mg(2+), Mn(2+), Fe(2+), Ca(2+), Co(2+) and Zn(2+). Inhibited by the sulfhydryl blocking agent 5,5'-dithio-bis-(2-nitrobenzoate), SDS and N-bromosuccinimide. Unaffected by sodium azide and EDTA. Inactivated following treatment with diethyl pyrocarbonate; this inactivation is reversible by treatment with hydroxylamine.

SIP144C34EEA41E261C
type: Kinetics Annotation
measured affinity: 3.7 uM for 4-chlorobenzoyl-CoA
measured affinity: 4.2 uM for 4-bromobenzoyl-CoA
measured affinity: 6.5 uM for 4-iodobenzoyl-CoA
measured affinity: 10.4 uM for 2,4-dichlorobenzoyl-CoA
measured affinity: 42 uM for 3,4-dichlorobenzoyl-CoA
measured affinity: 30 uM for 4-chloro-2-nitrobenzoyl-CoA
measured affinity: 5.5 uM for 4-chloro-3-nitrobenzoyl-CoA
measured activity: 2.2 umol/min/mg enzyme with 4-chlorobenzoyl-CoA as substrate

SIP1ABB2AB519964882
type: PH Dependence Annotation
comment: Optimum pH is 10.0. Half maximum activity remains at pH 9.0 and 11.5. Stable from pH 6.5 to 11.0, activity is lost following 10 minutes incubation at pH 4.5 or 12.0.

SIP471147B6B6CF310F
type: Temperature Dependence Annotation
comment: Optimum temperature is 60 degrees Celsius. Retains 60% of maximum activity at 30 degrees Celsius and 65 degrees Celsius. After 15 minutes preincubation at 60 degrees Celsius 70% of the initial activity remains, 15 minutes preincubation at 65 degrees Celsius results in a total loss of activity.

SIP8571FEB4BF148B66
type: Subunit Annotation
comment: Homotetramer (PubMed:1610806). Homotetramer, homooctamer and larger multimers (PubMed:7579994). Homotrimer (PubMed:8679561).

SIP609DCB093442B320
type: Induction Annotation
comment: By 4-chlorobenzoyl-CoA, 4-iodobenzoyl-CoA or 4-bromobenzoyl-CoA.

SIP42DC221D75708BC0
type: Similarity Annotation
comment: Belongs to the enoyl-CoA hydratase/isomerase family.

PRO_0000401147
type: Chain Annotation
comment: 4-chlorobenzoyl coenzyme A dehalogenase
mass: 29802
range: 18354466844390702tt1tt269

18354466844390702tt1
type: Position
type: ExactPosition
position: 1
reference: A5JTM5-1

18354466844390702tt269
type: Position
type: ExactPosition
position: 269
reference: A5JTM5-1

SIP116AE435C5011537
type: Active Site Annotation
comment: Proton acceptor
range: 18354466844390702tt90tt90

18354466844390702tt90
type: Position
type: ExactPosition
position: 90
reference: A5JTM5-1

SIP4EEFB2C39BAB2969
type: Active Site Annotation
comment: Nucleophile
range: 18354466844390702tt145tt145

18354466844390702tt145
type: Position
type: ExactPosition
position: 145
reference: A5JTM5-1

SIP72250D18A1144401
type: Binding Site Annotation
comment: in other chain
ligand: SIP11C6EFF6FEA76027
range: 18354466844390702tt24tt24

18354466844390702tt24
type: Position
type: ExactPosition
position: 24
reference: A5JTM5-1

SIP9960A69EAC48615F
type: Binding Site Annotation
comment: in other chain
ligand: SIP11C6EFF6FEA76027
range: 18354466844390702tt62tt67

18354466844390702tt62
type: Position
type: ExactPosition
position: 62
reference: A5JTM5-1

18354466844390702tt67
type: Position
type: ExactPosition
position: 67
reference: A5JTM5-1

SIPE7471EE58645A777
type: Binding Site Annotation
comment: in other chain
ligand: SIP11C6EFF6FEA76027
range: 18354466844390702tt114tt114

18354466844390702tt114
type: Position
type: ExactPosition
position: 114
reference: A5JTM5-1

18354466844390702tt257
type: Position
type: ExactPosition
position: 257
reference: A5JTM5-1

SIP45C1479F2C34131B
type: Mutagenesis Annotation
comment: Does not strongly affect catalytic activity, but reduces substrate CoA binding.
substitution: K
substitution: L
range: 18354466844390702tt24tt24

SIP656DCB951EFC968E
type: Mutagenesis Annotation
comment: Forms inclusion bodies.
substitution: T
range: 18354466844390702tt34tt34

18354466844390702tt34
type: Position
type: ExactPosition
position: 34
reference: A5JTM5-1

SIP3441CE34937C24BE
type: Mutagenesis Annotation
comment: No effect on catalytic activity.
substitution: A
range: 18354466844390702tt43tt43

18354466844390702tt43
type: Position
type: ExactPosition
position: 43
reference: A5JTM5-1

SIP4BEA8E3DE3E3F94E
type: Mutagenesis Annotation
comment: No effect on catalytic activity.
substitution: A
range: 18354466844390702tt45tt45

18354466844390702tt45
type: Position
type: ExactPosition
position: 45
reference: A5JTM5-1

SIPEA4DB60636EB881A
type: Mutagenesis Annotation
comment: No effect on catalytic activity.
substitution: A
range: 18354466844390702tt46tt46

18354466844390702tt46
type: Position
type: ExactPosition
position: 46
reference: A5JTM5-1

SIP6FFB876436901148
type: Mutagenesis Annotation
comment: Yields insoluble protein.
substitution: A
substitution: I
substitution: P
range: 18354466844390702tt63tt63

18354466844390702tt63
type: Position
type: ExactPosition
position: 63
reference: A5JTM5-1

SIP7D837730B37639E6
type: Mutagenesis Annotation
comment: 30-fold reduction in catalytic activity, substrate benzoyl group binding is unaffected.
substitution: A
range: 18354466844390702tt64tt64

18354466844390702tt64
type: Position
type: ExactPosition
position: 64
reference: A5JTM5-1

SIPB379360C4B51478D
type: Mutagenesis Annotation
comment: Retains catalytic activity, but substrate benzoyl group binding is decreased.
substitution: L
range: 18354466844390702tt64tt64

SIPAC4B8EB7F6193159
type: Mutagenesis Annotation
comment: Severely reduces catalytic activity. Arylated intermediate does not accumulate.
substitution: P
range: 18354466844390702tt64tt64

SIP7FCF3FE724E07A19
type: Mutagenesis Annotation
comment: Catalytic activity is almost as efficient as wild type.
substitution: D
range: 18354466844390702tt65tt65

18354466844390702tt65
type: Position
type: ExactPosition
position: 65
reference: A5JTM5-1

SIPD33F937A70B5EF21
type: Mutagenesis Annotation
comment: Reduces substrate CoA binding.
substitution: K
range: 18354466844390702tt67tt67

SIP61BC28D472736170
type: Mutagenesis Annotation
comment: Forms inclusion bodies.
substitution: L
range: 18354466844390702tt67tt67

SIPDE3185066BF78A87
type: Mutagenesis Annotation
comment: No effect on catalytic activity.
substitution: T
range: 18354466844390702tt68tt68

18354466844390702tt68
type: Position
type: ExactPosition
position: 68
reference: A5JTM5-1

SIP365BD86C72979A75
type: Mutagenesis Annotation
comment: Loss of catalytic activity, substrate benzoyl group binding is not affected.
substitution: Q
range: 18354466844390702tt81tt81

18354466844390702tt81
type: Position
type: ExactPosition
position: 81
reference: A5JTM5-1

SIP0E8CEF697B4DB8C8
type: Mutagenesis Annotation
comment: Retains catalytic activity, but substrate benzoyl group binding is decreased.
substitution: L
range: 18354466844390702tt82tt82

18354466844390702tt82
type: Position
type: ExactPosition
position: 82
reference: A5JTM5-1

SIP6C74DB5C8A2A9FE6
type: Mutagenesis Annotation
comment: Retains catalytic activity, but substrate benzoyl group binding is decreased.
substitution: F
range: 18354466844390702tt89tt89

18354466844390702tt89
type: Position
type: ExactPosition
position: 89
reference: A5JTM5-1

SIPE29CCDAFF9C5D68A
type: Mutagenesis Annotation
comment: Reduced activity and substrate benzoyl group binding.
substitution: Y
range: 18354466844390702tt89tt89

SIP2EF8208755A33776
type: Mutagenesis Annotation
comment: Complete loss of catalytic activity (PubMed:8718880, PubMed:9063883). Significantly reduced activity (PubMed:11695894). Substrate binding is not significantly affected. Reduced arylated intermediate formation.
substitution: Q
range: 18354466844390702tt90tt90

SIP6E0E79F47FC5F05F
type: Mutagenesis Annotation
comment: No effect on catalytic activity.
substitution: Q
range: 18354466844390702tt94tt94

18354466844390702tt94
type: Position
type: ExactPosition
position: 94
reference: A5JTM5-1

SIP94FC8F3FA108F600
type: Mutagenesis Annotation
comment: Yields insoluble protein.
substitution: G
range: 18354466844390702tt112tt112

18354466844390702tt112
type: Position
type: ExactPosition
position: 112
reference: A5JTM5-1

SIP8C20DF1903DCDF34
type: Mutagenesis Annotation
comment: Protein precipitates upon purification.
substitution: S
range: 18354466844390702tt112tt112

SIP07E806EEEB999D3A
type: Mutagenesis Annotation
comment: Catalytic activity is almost as efficient as wild type.
substitution: V
range: 18354466844390702tt112tt112

SIPB3D6F502CB08744D
type: Mutagenesis Annotation
comment: Strongly reduced catalytic activity and substrate benzoyl group binding. Arylated intermediate does not accumulate.
substitution: A
range: 18354466844390702tt113tt113

18354466844390702tt113
type: Position
type: ExactPosition
position: 113
reference: A5JTM5-1

SIPD1C431BF7CE54D0D
type: Mutagenesis Annotation
comment: Strongly reduced catalytic activity. Arylated intermediate does not accumulate.
substitution: N
substitution: S
range: 18354466844390702tt113tt113

SIPE142112DD8EE0E2D
type: Mutagenesis Annotation
comment: Protein precipitates upon purification.
substitution: V
range: 18354466844390702tt113tt113

SIP50F0CD968E7AFAFC
type: Mutagenesis Annotation
comment: Strongly reduced catalytic activity and substrate benzoyl group binding.
substitution: A
range: 18354466844390702tt114tt114

SIPAA587BC6DBA4D7C9
type: Mutagenesis Annotation
comment: Unstable.
substitution: P
range: 18354466844390702tt114tt114

SIP183E206360EAF090
type: Mutagenesis Annotation
comment: Yields insoluble protein.
substitution: L
substitution: N
substitution: S
substitution: V
range: 18354466844390702tt115tt115

18354466844390702tt115
type: Position
type: ExactPosition
position: 115
reference: A5JTM5-1

SIPA2B724F14F4713BC
type: Mutagenesis Annotation
comment: No effect on catalytic activity.
substitution: T
range: 18354466844390702tt123tt123

18354466844390702tt123
type: Position
type: ExactPosition
position: 123
reference: A5JTM5-1

SIPD56CA2C388E5EDA9
type: Mutagenesis Annotation
comment: No effect on catalytic activity.
substitution: T
range: 18354466844390702tt129tt129

18354466844390702tt129
type: Position
type: ExactPosition
position: 129
reference: A5JTM5-1

SIPD9103B1547679BB1
type: Mutagenesis Annotation
comment: Low catalytic activity, but KM unaffected (PubMed:8718880). Retains catalytic activity, but substrate benzoyl group binding is decreased (PubMed:9063883).
substitution: F
range: 18354466844390702tt137tt137

18354466844390702tt137
type: Position
type: ExactPosition
position: 137
reference: A5JTM5-1

SIP6ED74020467F6DEA
type: Mutagenesis Annotation
comment: Complete loss of catalytic activity, but not substrate binding.
substitution: A
range: 18354466844390702tt145tt145

SIP5314A3BDE3FD6F67
type: Mutagenesis Annotation
comment: No effect on catalytic activity.
substitution: T
range: 18354466844390702tt163tt163

18354466844390702tt163
type: Position
type: ExactPosition
position: 163
reference: A5JTM5-1

SIP40D71AED75F0057D
type: Mutagenesis Annotation
comment: No effect on catalytic activity.
substitution: D
range: 18354466844390702tt175tt175

18354466844390702tt175
type: Position
type: ExactPosition
position: 175
reference: A5JTM5-1

SIP80207CF1BCB98B4D
type: Mutagenesis Annotation
comment: No effect on catalytic activity.
substitution: F
range: 18354466844390702tt179tt179

18354466844390702tt179
type: Position
type: ExactPosition
position: 179
reference: A5JTM5-1

SIP56AA8867394630F0
type: Mutagenesis Annotation
comment: No effect on catalytic activity.
substitution: Q
range: 18354466844390702tt208tt208

18354466844390702tt208
type: Position
type: ExactPosition
position: 208
reference: A5JTM5-1

SIP7B1DE0A1E776A1F6
type: Mutagenesis Annotation
comment: Yields insoluble protein.
substitution: E
substitution: K
substitution: L
range: 18354466844390702tt216tt216

18354466844390702tt216
type: Position
type: ExactPosition
position: 216
reference: A5JTM5-1

SIPD2D45012292FA539
type: Mutagenesis Annotation
comment: Yields insoluble protein.
substitution: A
substitution: N
substitution: Q
substitution: R
range: 18354466844390702tt232tt232

18354466844390702tt232
type: Position
type: ExactPosition
position: 232
reference: A5JTM5-1

SIP793699EEE1F0B1E8
type: Mutagenesis Annotation
comment: Reduced catalytic activity, increased substrate binding.
substitution: D
range: 18354466844390702tt232tt232

SIP1AA9DCE5B2E2B0BD
type: Mutagenesis Annotation
comment: Retains catalytic activity and substrate CoA binding.
substitution: K
range: 18354466844390702tt257tt257

SIP37D8C500FCCFCC74
type: Mutagenesis Annotation
comment: Significantly reduces catalytic activity and substrate CoA binding.
substitution: L
range: 18354466844390702tt257tt257

18354466844390702tt3
type: Position
type: ExactPosition
position: 3
reference: A5JTM5-1

18354466844390702tt10
type: Position
type: ExactPosition
position: 10
reference: A5JTM5-1

18354466844390702tt13
type: Position
type: ExactPosition
position: 13
reference: A5JTM5-1

18354466844390702tt18
type: Position
type: ExactPosition
position: 18
reference: A5JTM5-1

18354466844390702tt21
type: Position
type: ExactPosition
position: 21
reference: A5JTM5-1

18354466844390702tt23
type: Position
type: ExactPosition
position: 23
reference: A5JTM5-1

18354466844390702tt29
type: Position
type: ExactPosition
position: 29
reference: A5JTM5-1

18354466844390702tt44
type: Position
type: ExactPosition
position: 44
reference: A5JTM5-1

18354466844390702tt50
type: Position
type: ExactPosition
position: 50
reference: A5JTM5-1

18354466844390702tt56
type: Position
type: ExactPosition
position: 56
reference: A5JTM5-1

18354466844390702tt59
type: Position
type: ExactPosition
position: 59
reference: A5JTM5-1

18354466844390702tt61
type: Position
type: ExactPosition
position: 61
reference: A5JTM5-1

18354466844390702tt66
type: Position
type: ExactPosition
position: 66
reference: A5JTM5-1

18354466844390702tt71
type: Position
type: ExactPosition
position: 71
reference: A5JTM5-1

18354466844390702tt73
type: Position
type: ExactPosition
position: 73
reference: A5JTM5-1

18354466844390702tt74
type: Position
type: ExactPosition
position: 74
reference: A5JTM5-1

18354466844390702tt98
type: Position
type: ExactPosition
position: 98
reference: A5JTM5-1

18354466844390702tt99
type: Position
type: ExactPosition
position: 99
reference: A5JTM5-1

18354466844390702tt101
type: Position
type: ExactPosition
position: 101
reference: A5JTM5-1

18354466844390702tt103
type: Position
type: ExactPosition
position: 103
reference: A5JTM5-1

18354466844390702tt107
type: Position
type: ExactPosition
position: 107
reference: A5JTM5-1

18354466844390702tt109
type: Position
type: ExactPosition
position: 109
reference: A5JTM5-1

18354466844390702tt121
type: Position
type: ExactPosition
position: 121
reference: A5JTM5-1

18354466844390702tt122
type: Position
type: ExactPosition
position: 122
reference: A5JTM5-1

18354466844390702tt128
type: Position
type: ExactPosition
position: 128
reference: A5JTM5-1

18354466844390702tt132
type: Position
type: ExactPosition
position: 132
reference: A5JTM5-1

18354466844390702tt134
type: Position
type: ExactPosition
position: 134
reference: A5JTM5-1

18354466844390702tt140
type: Position
type: ExactPosition
position: 140
reference: A5JTM5-1

18354466844390702tt148
type: Position
type: ExactPosition
position: 148
reference: A5JTM5-1

18354466844390702tt167
type: Position
type: ExactPosition
position: 167
reference: A5JTM5-1

18354466844390702tt173
type: Position
type: ExactPosition
position: 173
reference: A5JTM5-1

18354466844390702tt178
type: Position
type: ExactPosition
position: 178
reference: A5JTM5-1

18354466844390702tt183
type: Position
type: ExactPosition
position: 183
reference: A5JTM5-1

18354466844390702tt186
type: Position
type: ExactPosition
position: 186
reference: A5JTM5-1

18354466844390702tt188
type: Position
type: ExactPosition
position: 188
reference: A5JTM5-1

18354466844390702tt204
type: Position
type: ExactPosition
position: 204
reference: A5JTM5-1

18354466844390702tt207
type: Position
type: ExactPosition
position: 207
reference: A5JTM5-1

18354466844390702tt220
type: Position
type: ExactPosition
position: 220
reference: A5JTM5-1

18354466844390702tt225
type: Position
type: ExactPosition
position: 225
reference: A5JTM5-1

18354466844390702tt241
type: Position
type: ExactPosition
position: 241
reference: A5JTM5-1

18354466844390702tt245
type: Position
type: ExactPosition
position: 245
reference: A5JTM5-1

18354466844390702tt253
type: Position
type: ExactPosition
position: 253
reference: A5JTM5-1

SIP11C6EFF6FEA76027
subclass of: substrate
comment: ligand shared between two oligomeric partners

A5JTM5-1
type: Simple Sequence
modified: 2007-06-26
version: 1
mass: 29802
value: MYEAIGHRVEDGVAEITIKLPRHRNALSVKAMQEVTDALNRAEEDDSVGAVMITGAEDAFCAGFYLREIPLDKGVAGVRDHFRIGALWWHQMIHKIIRVKRPVLAAINGVAAGGGLGISLASDMAICADSAKFVCAWHTIGIGNDTATSYSLARIVGMRRAMELMLTNRTLYPEEAKDWGLVSRVYPKDDFREVAWKVARELAAAPTHLQVMAKERFHAGWMQPVEECTEFEIQNVIASVTHPHFMPCLTEFLDGHRADRPQVELPAGV

http://purl.uniprot.org/uniprot/A5JTM5#attribution-CE2051493E3509DECD79F5306DBA6848
creator: UniProtKB
manual: true
evidence: ECO_0000314
source: http://purl.uniprot.org/citations/7579994

http://purl.uniprot.org/uniprot/A5JTM5#attribution-9BD82EDBA11B5738907FB4A9D8E9030E
manual: true
evidence: ECO_0000250
source: O85078

http://purl.uniprot.org/uniprot/A5JTM5#attribution-3A2B987566E213BD3BB7A62C079D4161
manual: true
evidence: ECO_0000255

http://purl.uniprot.org/uniprot/A5JTM5#attribution-45AA31476E7C7ED913A4042E6E389599
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/11695894

http://purl.uniprot.org/uniprot/A5JTM5#attribution-5DC17EBCAA748026D665FB659821E408
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/11747444

http://purl.uniprot.org/uniprot/A5JTM5#attribution-0E6E6D2D16F91D01BFCDE8F824FF9B2B
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/12899635

http://purl.uniprot.org/uniprot/A5JTM5#attribution-261DE5EDBF225C49BD16BC0CD1196E5E
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/1610806

http://purl.uniprot.org/uniprot/A5JTM5#attribution-6596C1435CAC61706FB12DABABDE67D9
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/16388585

http://purl.uniprot.org/uniprot/A5JTM5#attribution-469AE044B87CC99AFBA1B05092096048
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/7579994

http://purl.uniprot.org/uniprot/A5JTM5#attribution-F0CE7A4811D90900D8FA409E3E333470
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/8218302

http://purl.uniprot.org/uniprot/A5JTM5#attribution-C3026F639E55D772E54573E93D2C060D
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/8679561

http://purl.uniprot.org/uniprot/A5JTM5#attribution-A27300A0A411DC55290098FDCA48A4DC
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/8718880

http://purl.uniprot.org/uniprot/A5JTM5#attribution-184F0D2ADFF96FB359B8A514A69FB5FE
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/9063883

http://purl.uniprot.org/uniprot/A5JTM5#attribution-291C707A01E6FC55ACD081F9A059DFA0
manual: true
evidence: ECO_0000303
source: http://purl.uniprot.org/citations/1610806

http://purl.uniprot.org/uniprot/A5JTM5#attribution-726E62FBCE8F6F7C353F0EFE25D395D4
manual: true
evidence: ECO_0000303
source: http://purl.uniprot.org/citations/7579994

http://purl.uniprot.org/uniprot/A5JTM5#attribution-2516CBE0764E2D2D5023E3EC6AE5E7F5
manual: true
evidence: ECO_0000305

http://purl.uniprot.org/uniprot/A5JTM5#attribution-09FCD8A7145D57E4697C8B84F75214AE
manual: true
evidence: ECO_0000312
source: ABQ44578.1

P06621
type: Protein
reviewed: true
created: 1988-01-01
modified: 2023-05-03
version: 112
mnemonic: CBPG_PSES6
old mnemonic: CBPG_PSESP
citation: http://purl.uniprot.org/citations/6396165
citation: SIPC6E659A3A04F6A95
citation: http://purl.uniprot.org/citations/3838935
citation: http://purl.uniprot.org/citations/9083113
recommended name: SIPBB444374D5C7FFD5
alternative name: SIPEF642D078F43944A
alternative name: SIP2958E8CB51B35344
alternative name: SIP1499401E81109998
alternative name: SIPEB79CC1D3EC41D5E
enzyme: http://purl.uniprot.org/enzyme/3.4.17.11
organism: http://purl.uniprot.org/taxonomy/312
annotation: SIPD273515959700130
annotation: SIP30F0E7AA95608AE0
annotation: SIP4E0C717A9D0CD62D
annotation: SIP2496A67AF46A58E6
annotation: SIP6C3EA9352DACE190
annotation: SIPFF4F8F0BD0032EE4
annotation: SIP8B75ABBC1330277C
annotation: PRO_0000026808
annotation: SIP0D75CD1DB3338851
annotation: SIP7D33E3EF8CFB3D95
annotation: SIPC6B4E4F4572E8F08
annotation: SIP1B83275DDDA4C03C
annotation: SIP3AFBED2B897DC412
annotation: SIPDC0758F3EDF01533
annotation: SIP9252731284D9112D
annotation: SIPB55AA4DB8BD490AA
annotation: SIP4ED43848769F99E5
annotation: SIP0194D7ADFCDEC0BB
annotation: SIP7659B576B1873ADC
annotation: SIPD7E2EC991A09705B
annotation: SIP3D8EAB0AFA2979B7
annotation: SIPC7E12D7EACCBAB2E
annotation: SIP26E243AE1EC7A032
annotation: SIPA710792BD7C77827
annotation: SIP0CC58D45A14D4363
annotation: SIP4CC703E661F18795
annotation: SIP069B370002F6958C
annotation: SIP111C8251FD64E5A1
annotation: SIPE271C71F43AE3781
annotation: SIPBF98B13C060C5A33
annotation: SIP8DFD8183A71BE4C0
annotation: SIPF874AD01715E2231
annotation: SIPB258B0913C4EA772
annotation: SIP4C536A2CB0F2CF86
annotation: SIPC397C870D8871347
annotation: SIP1F920B3FFD254218
annotation: SIPE38D3EABF63BDABB
annotation: SIPD44EB46338BC56AD
annotation: SIP508F9216FF8687D7
annotation: SIP5DE150079FB788B1
annotation: SIP2CACE70A8941D16C
annotation: SIP0B9AAC7D8C885F48
annotation: SIP7C88F4D792225357
annotation: SIP1F4829D90A705466
annotation: SIP4F7F3BFDD4FF4EAB
annotation: SIP729B4B503DAA8159
annotation: SIP570D534646A6F3B7
annotation: SIP9C3F9FC5D60CA2C8
annotation: SIP3E83BFA3F7E0BF89
annotation: SIP92BB14982DDDD3FE
annotation: SIPE9BA99034F96EB71
annotation: SIP58019CE6374CD943
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/2
classified with: http://purl.uniprot.org/keywords/121
classified with: http://purl.uniprot.org/keywords/903
classified with: http://purl.uniprot.org/keywords/479
classified with: http://purl.uniprot.org/keywords/482
classified with: http://purl.uniprot.org/keywords/582
classified with: http://purl.uniprot.org/keywords/732
classified with: http://purl.uniprot.org/keywords/862
classified with: GO_0004180

P06621-citation-6396165
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
scope: PARTIAL PROTEIN SEQUENCE

P06621-citation-SIPC6E659A3A04F6A95
type: Citation Statement
scope: ERRATUM OF PUBMED:6396165

P06621-citation-3838935
type: Citation Statement
scope: CHARACTERIZATION

P06621-citation-9083113
type: Citation Statement
scope: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 23-415 IN COMPLEX WITH ZINC
scope: COFACTOR
scope: ACTIVE SITE

A24955
type: Resource
database: PIR
comment: YXPSF2

1CG2
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.50
chain sequence mapping: PDB 1CG2 tt23tt415

PDB 1CG2 tt23tt415
chain: A/B/C/D=23-415

6XJ5
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 3.11
chain sequence mapping: PDB 6XJ5 tt24tt415

PDB 6XJ5 tt24tt415
chain: A/B/C/D/E/F/G/H=24-415

7M6U
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.59
chain sequence mapping: PDB 7M6U tt25tt415

PDB 7M6U tt25tt415
chain: A/B/C/D=25-415

DB00563
type: Resource
database: DrugBank
comment: Methotrexate

http://purl.uniprot.org/brenda/3.4.17.11
type: Resource
database: BRENDA
comment: 5085

cd03885
type: Resource
database: CDD
comment: M20_CPDG2
signature sequence match: CDD cd03885 match 1

http://purl.uniprot.org/gene3d/3.40.630.10
type: Resource
database: Gene3D
comment: Zn peptidases
signature sequence match: Gene3D 3.40.630.10 match 1

IPR001261
type: Resource
database: InterPro
comment: ArgE/DapE_CS

IPR036264
type: Resource
database: InterPro
comment: Bact_exopeptidase_dim_dom

IPR017150
type: Resource
database: InterPro
comment: Pept_M20_glutamate_carboxypep

IPR002933
type: Resource
database: InterPro
comment: Peptidase_M20

IPR011650
type: Resource
database: InterPro
comment: Peptidase_M20_dimer

PTHR43808
type: Resource
database: PANTHER
comment: ACETYLORNITHINE DEACETYLASE
signature sequence match: PANTHER PTHR43808 match 1

PTHR43808:SF10
type: Resource
database: PANTHER
comment: BLL3749 PROTEIN
signature sequence match: PANTHER PTHR43808:SF10 match 1

PF07687
type: Resource
database: Pfam
comment: M20_dimer
signature sequence match: Pfam PF07687 match 1

PF01546
type: Resource
database: Pfam
comment: Peptidase_M20
signature sequence match: Pfam PF01546 match 1

PIRSF037238
type: Resource
database: PIRSF
comment: Carboxypeptidase_G2
signature sequence match: PIRSF PIRSF037238 match 1

SSF55031
type: Resource
database: SUPFAM
comment: Bacterial exopeptidase dimerisation domain
signature sequence match: SUPFAM SSF55031 match 1

SSF53187
type: Resource
database: SUPFAM
comment: Zn-dependent exopeptidases
signature sequence match: SUPFAM SSF53187 match 1

PS00758
type: Resource
database: PROSITE
comment: ARGE_DAPE_CPG2_1
signature sequence match: PROSITE PS00758 match 1

PS00759
type: Resource
database: PROSITE
comment: ARGE_DAPE_CPG2_2
signature sequence match: PROSITE PS00759 match 1

SIPBB444374D5C7FFD5
type: Structured Name
full name: Carboxypeptidase G2
short name: CPDG2
ec name: 3.4.17.11

SIPEF642D078F43944A
type: Structured Name
full name: Folate hydrolase G2

SIP2958E8CB51B35344
type: Structured Name
full name: Glutamate carboxypeptidase

SIP1499401E81109998
type: Structured Name
full name: Pteroylmonoglutamic acid hydrolase G2

SIPEB79CC1D3EC41D5E
type: Structured Name
international nonproprietary name: Glucarpidase

cpg2
type: Gene

SIPD273515959700130
type: Function Annotation
comment: Catalyzes the hydrolysis of reduced and non-reduced folates to pteroates and L-glutamate. This enzyme has a broad specificity.

SIP4E0C717A9D0CD62D
type: Cofactor Annotation
comment: Binds 2 Zn(2+) ions per subunit.
cofactor: CHEBI_29105

SIP2496A67AF46A58E6
type: Subunit Annotation
comment: Homodimer.

SIP6C3EA9352DACE190
type: Pharmaceutical Annotation
comment: Used clinically as a folate-depleting, antitumor agent.

SIPFF4F8F0BD0032EE4
type: Similarity Annotation
comment: Belongs to the peptidase M20A family.

22571007532478766tt1
type: Position
type: ExactPosition
position: 1
reference: P06621-1

22571007532478766tt22
type: Position
type: ExactPosition
position: 22
reference: P06621-1

PRO_0000026808
type: Chain Annotation
comment: Carboxypeptidase G2
mass: 41695
range: 22571007532478766tt23tt415

22571007532478766tt23
type: Position
type: ExactPosition
position: 23
reference: P06621-1

22571007532478766tt415
type: Position
type: ExactPosition
position: 415
reference: P06621-1

22571007532478766tt114
type: Position
type: ExactPosition
position: 114
reference: P06621-1

SIP7D33E3EF8CFB3D95
type: Active Site Annotation
comment: Proton acceptor
range: 22571007532478766tt175tt175

22571007532478766tt175
type: Position
type: ExactPosition
position: 175
reference: P06621-1

22571007532478766tt112
type: Position
type: ExactPosition
position: 112
reference: P06621-1

22571007532478766tt141
type: Position
type: ExactPosition
position: 141
reference: P06621-1

22571007532478766tt176
type: Position
type: ExactPosition
position: 176
reference: P06621-1

22571007532478766tt200
type: Position
type: ExactPosition
position: 200
reference: P06621-1

22571007532478766tt385
type: Position
type: ExactPosition
position: 385
reference: P06621-1

22571007532478766tt30
type: Position
type: ExactPosition
position: 30
reference: P06621-1

22571007532478766tt51
type: Position
type: ExactPosition
position: 51
reference: P06621-1

22571007532478766tt60
type: Position
type: ExactPosition
position: 60
reference: P06621-1

22571007532478766tt76
type: Position
type: ExactPosition
position: 76
reference: P06621-1

22571007532478766tt80
type: Position
type: ExactPosition
position: 80
reference: P06621-1

22571007532478766tt85
type: Position
type: ExactPosition
position: 85
reference: P06621-1

22571007532478766tt92
type: Position
type: ExactPosition
position: 92
reference: P06621-1

22571007532478766tt100
type: Position
type: ExactPosition
position: 100
reference: P06621-1

22571007532478766tt107
type: Position
type: ExactPosition
position: 107
reference: P06621-1

22571007532478766tt121
type: Position
type: ExactPosition
position: 121
reference: P06621-1

22571007532478766tt124
type: Position
type: ExactPosition
position: 124
reference: P06621-1

22571007532478766tt128
type: Position
type: ExactPosition
position: 128
reference: P06621-1

22571007532478766tt130
type: Position
type: ExactPosition
position: 130
reference: P06621-1

22571007532478766tt133
type: Position
type: ExactPosition
position: 133
reference: P06621-1

22571007532478766tt135
type: Position
type: ExactPosition
position: 135
reference: P06621-1

22571007532478766tt137
type: Position
type: ExactPosition
position: 137
reference: P06621-1

22571007532478766tt142
type: Position
type: ExactPosition
position: 142
reference: P06621-1

22571007532478766tt143
type: Position
type: ExactPosition
position: 143
reference: P06621-1

22571007532478766tt158
type: Position
type: ExactPosition
position: 158
reference: P06621-1

22571007532478766tt164
type: Position
type: ExactPosition
position: 164
reference: P06621-1

22571007532478766tt173
type: Position
type: ExactPosition
position: 173
reference: P06621-1

22571007532478766tt177
type: Position
type: ExactPosition
position: 177
reference: P06621-1

22571007532478766tt178
type: Position
type: ExactPosition
position: 178
reference: P06621-1

22571007532478766tt182
type: Position
type: ExactPosition
position: 182
reference: P06621-1

22571007532478766tt183
type: Position
type: ExactPosition
position: 183
reference: P06621-1

22571007532478766tt192
type: Position
type: ExactPosition
position: 192
reference: P06621-1

22571007532478766tt194
type: Position
type: ExactPosition
position: 194
reference: P06621-1

22571007532478766tt198
type: Position
type: ExactPosition
position: 198
reference: P06621-1

22571007532478766tt208
type: Position
type: ExactPosition
position: 208
reference: P06621-1

22571007532478766tt213
type: Position
type: ExactPosition
position: 213
reference: P06621-1

22571007532478766tt215
type: Position
type: ExactPosition
position: 215
reference: P06621-1

22571007532478766tt224
type: Position
type: ExactPosition
position: 224
reference: P06621-1

22571007532478766tt230
type: Position
type: ExactPosition
position: 230
reference: P06621-1

22571007532478766tt232
type: Position
type: ExactPosition
position: 232
reference: P06621-1

22571007532478766tt234
type: Position
type: ExactPosition
position: 234
reference: P06621-1

22571007532478766tt236
type: Position
type: ExactPosition
position: 236
reference: P06621-1

22571007532478766tt240
type: Position
type: ExactPosition
position: 240
reference: P06621-1

22571007532478766tt251
type: Position
type: ExactPosition
position: 251
reference: P06621-1

22571007532478766tt252
type: Position
type: ExactPosition
position: 252
reference: P06621-1

22571007532478766tt254
type: Position
type: ExactPosition
position: 254
reference: P06621-1

22571007532478766tt257
type: Position
type: ExactPosition
position: 257
reference: P06621-1

22571007532478766tt260
type: Position
type: ExactPosition
position: 260
reference: P06621-1

22571007532478766tt261
type: Position
type: ExactPosition
position: 261
reference: P06621-1

22571007532478766tt270
type: Position
type: ExactPosition
position: 270
reference: P06621-1

22571007532478766tt279
type: Position
type: ExactPosition
position: 279
reference: P06621-1

22571007532478766tt291
type: Position
type: ExactPosition
position: 291
reference: P06621-1

22571007532478766tt292
type: Position
type: ExactPosition
position: 292
reference: P06621-1

22571007532478766tt306
type: Position
type: ExactPosition
position: 306
reference: P06621-1

22571007532478766tt315
type: Position
type: ExactPosition
position: 315
reference: P06621-1

22571007532478766tt322
type: Position
type: ExactPosition
position: 322
reference: P06621-1

22571007532478766tt330
type: Position
type: ExactPosition
position: 330
reference: P06621-1

22571007532478766tt346
type: Position
type: ExactPosition
position: 346
reference: P06621-1

22571007532478766tt352
type: Position
type: ExactPosition
position: 352
reference: P06621-1

22571007532478766tt355
type: Position
type: ExactPosition
position: 355
reference: P06621-1

22571007532478766tt356
type: Position
type: ExactPosition
position: 356
reference: P06621-1

22571007532478766tt358
type: Position
type: ExactPosition
position: 358
reference: P06621-1

22571007532478766tt363
type: Position
type: ExactPosition
position: 363
reference: P06621-1

22571007532478766tt366
type: Position
type: ExactPosition
position: 366
reference: P06621-1

22571007532478766tt367
type: Position
type: ExactPosition
position: 367
reference: P06621-1

22571007532478766tt369
type: Position
type: ExactPosition
position: 369
reference: P06621-1

22571007532478766tt380
type: Position
type: ExactPosition
position: 380
reference: P06621-1

22571007532478766tt382
type: Position
type: ExactPosition
position: 382
reference: P06621-1

22571007532478766tt386
type: Position
type: ExactPosition
position: 386
reference: P06621-1

22571007532478766tt388
type: Position
type: ExactPosition
position: 388
reference: P06621-1

22571007532478766tt391
type: Position
type: ExactPosition
position: 391
reference: P06621-1

22571007532478766tt393
type: Position
type: ExactPosition
position: 393
reference: P06621-1

22571007532478766tt394
type: Position
type: ExactPosition
position: 394
reference: P06621-1

22571007532478766tt396
type: Position
type: ExactPosition
position: 396
reference: P06621-1

22571007532478766tt397
type: Position
type: ExactPosition
position: 397
reference: P06621-1

22571007532478766tt412
type: Position
type: ExactPosition
position: 412
reference: P06621-1

2
subclass of: CHEBI_29105

1
subclass of: CHEBI_29105

P06621-1
type: Simple Sequence
modified: 1988-01-01
version: 1
precursor: true
mass: 43932
value: MRPSIHRTAIAAVLATAFVAGTALAQKRDNVLFQAATDEQPAVIKTLEKLVNIETGTGDAEGIAAAGNFLEAELKNLGFTVTRSKSAGLVVGDNIVGKIKGRGGKNLLLMSHMDTVYLKGILAKAPFRVEGDKAYGPGIADDKGGNAVILHTLKLLKEYGVRDYGTITVLFNTDEEKGSFGSRDLIQEEAKLADYVLSFEPTSAGDEKLSLGTSGIAYVQVNITGKASHAGAAPELGVNALVEASDLVLRTMNIDDKAKNLRFNWTIAKAGNVSNIIPASATLNADVRYARNEDFDAAMKTLEERAQQKKLPEADVKVIVTRGRPAFNAGEGGKKLVDKAVAYYKEAGGTLGVEERTGGGTDAAYAALSGKPVIESLGLPGFGYHSDKAEYVDISAIPRRLYMAARLIMDLGAGK

http://purl.uniprot.org/uniprot/P06621#attribution-609619A7DF3E53CE5E48EDEF8B8F9DD1
manual: true
evidence: ECO_0000250

http://purl.uniprot.org/uniprot/P06621#attribution-308AEA4B1190EFCD85C1415B5DF7D8E4
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/9083113

http://purl.uniprot.org/uniprot/P06621#attribution-080ED3982FEC43F7C43A0689717F3D20
manual: true
evidence: ECO_0000305

P96977
type: Protein
reviewed: true
created: 1998-07-15
modified: 2023-05-03
version: 57
mnemonic: CHRR_PSEUG
old mnemonic: CHRR_PSESP
citation: SIP654B9FD9FD2CF1FF
recommended name: SIP4EF0FE8EF6F42DEE
enzyme: http://purl.uniprot.org/enzyme/1.-.-.-
organism: http://purl.uniprot.org/taxonomy/79677
annotation: SIP59E46FDF5A5A6961
annotation: SIP80640135AE99DEDC
annotation: PRO_0000205509
existence: Inferred from Homology Existence
classified with: http://purl.uniprot.org/keywords/288
classified with: http://purl.uniprot.org/keywords/560
classified with: GO_0016491
sequence: P96977-1

SIP654B9FD9FD2CF1FF
type: Submission Citation
author: Suzuki T.
date: 1996-02
submitted to: EMBL/GenBank/DDBJ

P96977-citation-SIP654B9FD9FD2CF1FF
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

cd02146
type: Resource
database: CDD
comment: NfsA-like
signature sequence match: CDD cd02146 match 1

http://purl.uniprot.org/gene3d/3.40.109.10
type: Resource
database: Gene3D
comment: NADH Oxidase
signature sequence match: Gene3D 3.40.109.10 match 1

IPR016446
type: Resource
database: InterPro
comment: Flavin_OxRdtase_Frp

IPR029479
type: Resource
database: InterPro
comment: Nitroreductase

IPR000415
type: Resource
database: InterPro
comment: Nitroreductase-like

PTHR43425:SF3
type: Resource
database: PANTHER
comment: FMN REDUCTASE (NADPH)
signature sequence match: PANTHER PTHR43425:SF3 match 1

PTHR43425
type: Resource
database: PANTHER
comment: OXYGEN-INSENSITIVE NADPH NITROREDUCTASE
signature sequence match: PANTHER PTHR43425 match 1

PF00881
type: Resource
database: Pfam
comment: Nitroreductase
signature sequence match: Pfam PF00881 match 1

PIRSF005426
type: Resource
database: PIRSF
comment: Frp
signature sequence match: PIRSF PIRSF005426 match 1

SSF55469
type: Resource
database: SUPFAM
comment: FMN-dependent nitroreductase-like
signature sequence match: SUPFAM SSF55469 match 1

SIP4EF0FE8EF6F42DEE
type: Structured Name
full name: CR(VI) reductase
ec name: 1.-.-.-

chrR
type: Gene

SIP80640135AE99DEDC
type: Similarity Annotation
comment: Belongs to the flavin oxidoreductase frp family.

PRO_0000205509
type: Chain Annotation
comment: CR(VI) reductase
mass: 27540
range: 22580903187789614tt1tt243

22580903187789614tt1
type: Position
type: ExactPosition
position: 1
reference: P96977-1

22580903187789614tt243
type: Position
type: ExactPosition
position: 243
reference: P96977-1

P96977-1
type: Simple Sequence
modified: 1997-05-01
version: 1
mass: 27540
value: MVKELLRNHSSVRIYDGNPISKEIIEELIATAQMAATSHFVQAYSVIWVTDEEKKEKLGMLSGNPRQYETSGGAFVFCVDFKRLQSAGKLEGVDIVADSAENVLVGVADVSLFAQNFVVAAESMGYGICYIGGVRNKPEEISELFNLPEYVFPLFGLTIGVPARRNEVKPRLPVAAVLHENEYNTEKYEELLPAYNDTMEAYYNNRSSNRKIDNWTKQMADFLIEQRRPHIKDFLAKKGFNWK

http://purl.uniprot.org/uniprot/P96977#attribution-609619A7DF3E53CE5E48EDEF8B8F9DD1
manual: true
evidence: ECO_0000250

http://purl.uniprot.org/uniprot/P96977#attribution-080ED3982FEC43F7C43A0689717F3D20
manual: true
evidence: ECO_0000305

P17262
type: Protein
reviewed: true
created: 1990-08-01
modified: 2023-05-03
version: 94
mnemonic: DMPB_PSEUF
old mnemonic: DMPB_PSEPU
old mnemonic: DMPB_PSESP
citation: http://purl.uniprot.org/citations/2620833
citation: http://purl.uniprot.org/citations/2194577
recommended name: SIPAE6BB3ABA6E5EDDD
alternative name: SIP1AA450B16B071666
alternative name: SIP9AD16B8F141FBA49
enzyme: http://purl.uniprot.org/enzyme/1.13.11.2
organism: http://purl.uniprot.org/taxonomy/79676
encoded in: pVI150
annotation: SIPBBB7469141831193
annotation: SIPAE0E2758BE6B33B4
annotation: SIPFE522E06418852AA
annotation: SIP45F47F4BD50DBE23
annotation: SIP220B6481128035CF
annotation: PRO_0000085029
annotation: SIP4C37FD74A90775BC
annotation: SIPBAC09F4D08B24292
annotation: SIP105913888C659B9D
annotation: SIP1103B924FC716139
annotation: SIP471A94397AB893F6
existence: Inferred from Homology Existence
classified with: http://purl.uniprot.org/keywords/58
classified with: http://purl.uniprot.org/keywords/223
classified with: http://purl.uniprot.org/keywords/408
classified with: http://purl.uniprot.org/keywords/479
classified with: http://purl.uniprot.org/keywords/614
classified with: http://purl.uniprot.org/keywords/677
classified with: GO_0018577
classified with: GO_0008198
classified with: GO_0043640
sequence: P17262-1

P17262-citation-2620833
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

P17262-citation-2194577
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 287-307

cd07243
type: Resource
database: CDD
comment: 2_3_CTD_C
signature sequence match: CDD cd07243 match 1

cd07265
type: Resource
database: CDD
comment: 2_3_CTD_N
signature sequence match: CDD cd07265 match 1

IPR017624
type: Resource
database: InterPro
comment: Catechol_2-3_dOase

TIGR03211
type: Resource
database: TIGRFAMs
comment: catechol_2_3
signature sequence match: TIGRFAMs TIGR03211 match 1

SIPAE6BB3ABA6E5EDDD
type: Structured Name
full name: Metapyrocatechase
short name: MPC
ec name: 1.13.11.2

SIP1AA450B16B071666
type: Structured Name
full name: CatO2ase

SIP9AD16B8F141FBA49
type: Structured Name
full name: Catechol 2,3-dioxygenase
short name: C23O

pVI150
type: Plasmid

dmpB
type: Gene

SIP45F47F4BD50DBE23
type: Subunit Annotation
comment: Homotetramer.

SIP220B6481128035CF
type: Similarity Annotation
comment: Belongs to the extradiol ring-cleavage dioxygenase family.

PRO_0000085029
type: Chain Annotation
comment: Metapyrocatechase
mass: 35255
range: 22572111272227374tt1tt307

22572111272227374tt1
type: Position
type: ExactPosition
position: 1
reference: P17262-1

22572111272227374tt307
type: Position
type: ExactPosition
position: 307
reference: P17262-1

SIP4C37FD74A90775BC
type: Domain Extent Annotation
comment: VOC 1
range: 22572111272227374tt7tt122

22572111272227374tt7
type: Position
type: ExactPosition
position: 7
reference: P17262-1

22572111272227374tt122
type: Position
type: ExactPosition
position: 122
reference: P17262-1

SIPBAC09F4D08B24292
type: Domain Extent Annotation
comment: VOC 2
range: 22572111272227374tt150tt269

22572111272227374tt150
type: Position
type: ExactPosition
position: 150
reference: P17262-1

22572111272227374tt269
type: Position
type: ExactPosition
position: 269
reference: P17262-1

22572111272227374tt153
type: Position
type: ExactPosition
position: 153
reference: P17262-1

22572111272227374tt214
type: Position
type: ExactPosition
position: 214
reference: P17262-1

22572111272227374tt265
type: Position
type: ExactPosition
position: 265
reference: P17262-1

P17262-1
type: Simple Sequence
modified: 1990-08-01
version: 1
mass: 35255
value: MKKGVMRPGHVQLRVLNLESALAHYRDLLGLIEMDRDEQGRVYLKAWTEVDKFSVVLREADQPGMDFMGFKVIDEDCLNRLTQDLLNYGCLIETIPAGELKGCGRRVRFQTPSGHFFELYADKEYTGKWGLEEINPEAWPRNLKGMRAVRFDHCLLYGDELQATYALFTEVLGFYLAEQVIDDDGTRVAQFLSLSTKAHDVAFIHCPEKGKFHHVSFFLETWEDVLRAADLISMTDTSIDIGPTRHGLTHGKTIYFFDPSGNRNEVFCGGDYNYQDHKPVTWLAKDLGKAIFYHDRVLNERFLTVLT

http://purl.uniprot.org/uniprot/P17262#attribution-609619A7DF3E53CE5E48EDEF8B8F9DD1
manual: true
evidence: ECO_0000250

http://purl.uniprot.org/uniprot/P17262#attribution-05C16B64E576893F0FB5E6F0F0FB0983
manual: true
evidence: ECO_0000255
source: PRU01163

http://purl.uniprot.org/uniprot/P17262#attribution-080ED3982FEC43F7C43A0689717F3D20
manual: true
evidence: ECO_0000305

P19059
type: Protein
reviewed: true
created: 1990-11-01
modified: 2023-05-03
version: 88
mnemonic: DMPC_PSEUF
old mnemonic: DMPC_PSEPU
old mnemonic: DMPC_PSESP
citation: http://purl.uniprot.org/citations/2194577
recommended name: SIPCDB369B7BAE04F28
enzyme: http://purl.uniprot.org/enzyme/1.2.1.85
organism: http://purl.uniprot.org/taxonomy/79676
encoded in: pVI150
annotation: SIP3636CD9FFF259954
annotation: SIP0737CAEFC439FD6B
annotation: SIP06928ECB92FC99CE
annotation: SIP8C59C79F418631D7
annotation: PRO_0000056588
annotation: SIP209CC18EDA800FD1
annotation: SIP95C25E518CAC995F
existence: Inferred from Homology Existence
classified with: http://purl.uniprot.org/keywords/58
classified with: http://purl.uniprot.org/keywords/520
classified with: http://purl.uniprot.org/keywords/560
classified with: http://purl.uniprot.org/keywords/614
classified with: GO_0016620
classified with: GO_0043640
sequence: P19059-1

P19059-citation-2194577
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

cd07093
type: Resource
database: CDD
comment: ALDH_F8_HMSADH
signature sequence match: CDD cd07093 match 1

IPR016161
type: Resource
database: InterPro
comment: Ald_DH/histidinol_DH

IPR016163
type: Resource
database: InterPro
comment: Ald_DH_C

IPR016160
type: Resource
database: InterPro
comment: Ald_DH_CS_CYS

IPR029510
type: Resource
database: InterPro
comment: Ald_DH_CS_GLU

IPR016162
type: Resource
database: InterPro
comment: Ald_DH_N

IPR015590
type: Resource
database: InterPro
comment: Aldehyde_DH_dom

IPR017628
type: Resource
database: InterPro
comment: OHmuconic_semiald_DH

PTHR43720
type: Resource
database: PANTHER
comment: 2-AMINOMUCONIC SEMIALDEHYDE DEHYDROGENASE
signature sequence match: PANTHER PTHR43720 match 1

PTHR43720:SF2
type: Resource
database: PANTHER
comment: 2-AMINOMUCONIC SEMIALDEHYDE DEHYDROGENASE
signature sequence match: PANTHER PTHR43720:SF2 match 1

PF00171
type: Resource
database: Pfam
comment: Aldedh
signature sequence match: Pfam PF00171 match 1

SSF53720
type: Resource
database: SUPFAM
comment: ALDH-like
signature sequence match: SUPFAM SSF53720 match 1

TIGR03216
type: Resource
database: TIGRFAMs
comment: OH_muco_semi_DH
signature sequence match: TIGRFAMs TIGR03216 match 1

PS00070
type: Resource
database: PROSITE
comment: ALDEHYDE_DEHYDR_CYS
signature sequence match: PROSITE PS00070 match 1

PS00687
type: Resource
database: PROSITE
comment: ALDEHYDE_DEHYDR_GLU
signature sequence match: PROSITE PS00687 match 1

SIPCDB369B7BAE04F28
type: Structured Name
full name: 2-hydroxymuconic semialdehyde dehydrogenase
short name: HMSD
ec name: 1.2.1.85

pVI150
type: Plasmid

dmpC
type: Gene

SIP3636CD9FFF259954
type: Function Annotation
comment: 2-hydroxymuconic acid semialdehyde can be converted to 2-hydroxypent-2,4-dienoate either directly by the action of 2-hydroxymuconic semialdehyde hydrolase (HMSH) or by the action of three sequential enzymes, the first of which is HMSD.

SIP8C59C79F418631D7
type: Similarity Annotation
comment: Belongs to the aldehyde dehydrogenase family.

PRO_0000056588
type: Chain Annotation
comment: 2-hydroxymuconic semialdehyde dehydrogenase
mass: 51683
range: 22572119828543790tt1tt486

22572119828543790tt1
type: Position
type: ExactPosition
position: 1
reference: P19059-1

22572119828543790tt486
type: Position
type: ExactPosition
position: 486
reference: P19059-1

22572119828543790tt254
type: Position
type: ExactPosition
position: 254
reference: P19059-1

22572119828543790tt288
type: Position
type: ExactPosition
position: 288
reference: P19059-1

P19059-1
type: Simple Sequence
modified: 1990-11-01
version: 1
mass: 51683
value: MKEIKHFINGAFVGSASGRTFEDVNPANGQVIARVHEAGRAEVDAAVQAARAALKGPWGKMSVSERAEILHRVADGITARFDEFLEAECLDTGKPKSLASHIDIPRGAANFKVFADLLKNVATEAFEMATPDGSGAINYAVRRPKGVIGVISPWNLPLLLMTWKVGPALACGNTVVVKPSEETPLTTALLGEVMQAAGVPAGVYNVVHGFGPDSAGAFLTEHPDVNAITFTGETRTGEAIMRAAAKGVRPVSFELGGKNAGIVFADCDLDKAIEGSMRSVFANGGQVCLGTERLYVERPIFDEFVARLKAGAESLVIGTPDDPQANFGPLISLQHREKVLSYYQKAVDEGATVVTGGGVPEMPAELAGGAWVQPTIWTGLADGAAVVTEEIFGPCCHIRPFDREEEAVELANSLPYGLAATIWTENTSRAHRVAGQLEAGIVWVNSWFLRDLRTAFGGSKQSGIGREGGVHSLEFYTELKNICVKL

http://purl.uniprot.org/uniprot/P19059#attribution-609619A7DF3E53CE5E48EDEF8B8F9DD1
manual: true
evidence: ECO_0000250

http://purl.uniprot.org/uniprot/P19059#attribution-080ED3982FEC43F7C43A0689717F3D20
manual: true
evidence: ECO_0000305

P19076
type: Protein
reviewed: true
created: 1990-11-01
modified: 2023-05-03
version: 81
mnemonic: DMPD_PSEUF
old mnemonic: DMPD_PSEPU
old mnemonic: DMPD_PSESP
citation: http://purl.uniprot.org/citations/2194577
recommended name: SIP46B7E04B9132AA15
alternative name: SIPD35032829507AD3E
enzyme: http://purl.uniprot.org/enzyme/3.7.1.9
organism: http://purl.uniprot.org/taxonomy/79676
encoded in: pVI150
annotation: SIPE812E4CF3AB94FC0
annotation: SIP8E879FF7B2A58D73
annotation: SIP152C40ED42944A7E
annotation: SIPF3B95C444360A96B
annotation: PRO_0000207055
annotation: SIP882D1CD1A3C5B81E
annotation: SIPBA130E1C1B88D7A5
annotation: SIPA45BF72C4857060F
annotation: SIP49CE84C1E46726B2
existence: Inferred from Homology Existence
classified with: http://purl.uniprot.org/keywords/58
classified with: http://purl.uniprot.org/keywords/614
classified with: http://purl.uniprot.org/keywords/719
classified with: GO_0018775
classified with: GO_0052689
classified with: GO_0043640
sequence: P19076-1

P19076-citation-2194577
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

psesp-dmpd
type: Resource
database: ESTHER
comment: Carbon-carbon_bond_hydrolase

PTHR43798:SF31
type: Resource
database: PANTHER
comment: AB HYDROLASE SUPERFAMILY PROTEIN YCLE
signature sequence match: PANTHER PTHR43798:SF31 match 1

PTHR43798
type: Resource
database: PANTHER
comment: MONOACYLGLYCEROL LIPASE
signature sequence match: PANTHER PTHR43798 match 1

SIP46B7E04B9132AA15
type: Structured Name
full name: 2-hydroxymuconate semialdehyde hydrolase
short name: HMSH
ec name: 3.7.1.9

SIPD35032829507AD3E
type: Structured Name
full name: 2-hydroxymuconic semialdehyde hydrolase

pVI150
type: Plasmid

dmpD
type: Gene

SIPE812E4CF3AB94FC0
type: Function Annotation
comment: Catalyzes the conversion of 2-hydroxymuconate semialdehyde to 2-hydroxypent-2,4-dienoate.

SIPF3B95C444360A96B
type: Similarity Annotation
comment: Belongs to the DmpD/TodF/XylF esterase family.

PRO_0000207055
type: Chain Annotation
comment: 2-hydroxymuconate semialdehyde hydrolase
mass: 30965
range: 22572119828674094tt1tt283

22572119828674094tt1
type: Position
type: ExactPosition
position: 1
reference: P19076-1

22572119828674094tt283
type: Position
type: ExactPosition
position: 283
reference: P19076-1

SIP882D1CD1A3C5B81E
type: Domain Extent Annotation
comment: AB hydrolase-1
range: 22572119828674094tt32tt262

22572119828674094tt32
type: Position
type: ExactPosition
position: 32
reference: P19076-1

22572119828674094tt262
type: Position
type: ExactPosition
position: 262
reference: P19076-1

22572119828674094tt107
type: Position
type: ExactPosition
position: 107
reference: P19076-1

22572119828674094tt228
type: Position
type: ExactPosition
position: 228
reference: P19076-1

22572119828674094tt256
type: Position
type: ExactPosition
position: 256
reference: P19076-1

P19076-1
type: Simple Sequence
modified: 1990-11-01
version: 1
mass: 30965
value: MNAPQNSPEIGREIIAAGIRTNLHDSGAGFPLMMIHGSGPGVTAWANWRLVMPELAKSRRVIAPDMLGFGYSERPADAQYNRDVWVDHAVGVLDALEIEQADLVGNSFGGGIALALAIRHPERVRRLVLMGSAGVSFPITEGLDAVWGYNPSFAEMRRLLDIFAFDRNLVNDELAELRYQASIRPGFHESFAAMFPAPRQRWVDGLASAEAAIRALPHETLVIHGREDQIIPLQTSLTLADWIARAQLHVFGQCGHWTQIEHAARFASLVGDFLAEADAAAIS

http://purl.uniprot.org/uniprot/P19076#attribution-609619A7DF3E53CE5E48EDEF8B8F9DD1
manual: true
evidence: ECO_0000250

http://purl.uniprot.org/uniprot/P19076#attribution-46BAF9912BAC37AEF4C6D095313B9117
manual: true
evidence: ECO_0000255

http://purl.uniprot.org/uniprot/P19076#attribution-080ED3982FEC43F7C43A0689717F3D20
manual: true
evidence: ECO_0000305

P49156
type: Protein
reviewed: true
created: 1996-02-01
modified: 2023-05-03
version: 69
mnemonic: DMPH_PSEUF
old mnemonic: DMPH_PSEPU
old mnemonic: DMPH_PSESP
citation: http://purl.uniprot.org/citations/1732207
recommended name: SIPCB758D6F9BB61D35
enzyme: http://purl.uniprot.org/enzyme/4.1.1.77
organism: http://purl.uniprot.org/taxonomy/79676
encoded in: pVI150
annotation: SIP37359C5B6B36F672
annotation: SIP10E1DB09EF6586D6
annotation: SIPB116A7EA5AF12A21
annotation: PRO_0000079939
existence: Inferred from Homology Existence
classified with: http://purl.uniprot.org/keywords/58
classified with: http://purl.uniprot.org/keywords/456
classified with: http://purl.uniprot.org/keywords/614
classified with: GO_0047437
classified with: GO_0043640
sequence: P49156-1

P49156-citation-1732207
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

http://purl.uniprot.org/gene3d/3.90.850.10
type: Resource
database: Gene3D
comment: Fumarylacetoacetase-like, C-terminal domain
signature sequence match: Gene3D 3.90.850.10 match 1

IPR017630
type: Resource
database: InterPro
comment: 4-oxalocrotonate_decarboxylase

IPR036663
type: Resource
database: InterPro
comment: Fumarylacetoacetase_C_sf

PTHR30143:SF0
type: Resource
database: PANTHER
comment: 2-KETO-4-PENTENOATE HYDRATASE
signature sequence match: PANTHER PTHR30143:SF0 match 1

PTHR30143
type: Resource
database: PANTHER
comment: ACID HYDRATASE
signature sequence match: PANTHER PTHR30143 match 1

SSF56529
type: Resource
database: SUPFAM
comment: FAH
signature sequence match: SUPFAM SSF56529 match 1

TIGR03218
type: Resource
database: TIGRFAMs
comment: catechol_dmpH
signature sequence match: TIGRFAMs TIGR03218 match 1

SIPCB758D6F9BB61D35
type: Structured Name
full name: 4-oxalocrotonate decarboxylase
short name: 4-OD
ec name: 4.1.1.77

pVI150
type: Plasmid

dmpH
type: Gene

SIPB116A7EA5AF12A21
type: Similarity Annotation
comment: Belongs to the hydratase/decarboxylase family.

PRO_0000079939
type: Chain Annotation
comment: 4-oxalocrotonate decarboxylase
mass: 28427
range: 22575418380203566tt1tt264

22575418380203566tt1
type: Position
type: ExactPosition
position: 1
reference: P49156-1

22575418380203566tt264
type: Position
type: ExactPosition
position: 264
reference: P49156-1

P49156-1
type: Simple Sequence
modified: 1996-02-01
version: 1
mass: 28427
value: MNRTLTRDQVLALAEHIENAELDVHDIPKVTNDYPDMTFADAYDVQWEIRRRKEARGNKVVGLKMGLTSWAKMAQMGVETPIYGFLVDYFSVPDGGVVDTSKLIHPKIEAEISFVTKAPLHGPGCHIGQVLAATDFVIPTVEVIDSRYENFKFDLISVVADNASSTRFITGGQMANVADLDLRTLGVVMEKNGEVVELGAGAAVLGHPASSVAMLANLLAERGEHIPAGSFIMTGGITAAVPVAPGDNITVRYQGLCSVSARFI

http://purl.uniprot.org/uniprot/P49156#attribution-080ED3982FEC43F7C43A0689717F3D20
manual: true
evidence: ECO_0000305

P19729
type: Protein
reviewed: true
created: 1991-02-01
modified: 2023-05-03
version: 67
mnemonic: DMPK_PSEUF
old mnemonic: DMPK_PSEPU
old mnemonic: DMPK_PSESP
citation: http://purl.uniprot.org/citations/2254258
citation: SIPAF0C52FCB7DC8F06
citation: http://purl.uniprot.org/citations/2254259
citation: http://purl.uniprot.org/citations/8995386
recommended name: SIPB779B97DBE2D1AD2
alternative name: SIP5C258063F6453F82
alternative name: SIP8C6A2712A0BB7C0F
organism: http://purl.uniprot.org/taxonomy/79676
encoded in: pVI150
annotation: SIP24993B045B8B4E7F
annotation: SIPEAB183E7B9F35E1C
annotation: SIPA926BF039FF2E567
annotation: SIPCBE1A11D36AC633D
annotation: SIPC3BE56A4EFAD2F03
annotation: SIPBFB9280C529364D6
annotation: PRO_0000079940
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/58
classified with: http://purl.uniprot.org/keywords/614
classified with: GO_0018662
classified with: GO_0019336
sequence: MTVTNTPTPTFDQLTRYIRVRSEPEAKFVEFDFAIGHPELFVELVLPQDAFVKFCQHNRVVAMDEAMAKAVDDDMVKWRFGDVGRRLPKDPG

P19729-citation-2254258
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
scope: FUNCTION
scope: PATHWAY
scope: DISRUPTION PHENOTYPE

CF600
type: Strain

SIPAF0C52FCB7DC8F06
type: Submission Citation
author: Takeo M.
author: Maeda Y.
author: Okada H.
author: Miyama K.
author: Mori K.
author: Ike M.
author: Fujita M.
date: 1994-03
submitted to: EMBL/GenBank/DDBJ

P19729-citation-SIPAF0C52FCB7DC8F06
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

BH
type: Strain

P19729-citation-2254259
type: Citation Statement
scope: FUNCTION

P19729-citation-8995386
type: Citation Statement
scope: FUNCTION
scope: SUBUNIT
scope: INTERACTION WITH DMPL AND DMPN
scope: MASS SPECTROMETRY

http://purl.uniprot.org/brenda/1.14.13.244
type: Resource
database: BRENDA
comment: 16277

IPR010353
type: Resource
database: InterPro
comment: DmpK

PF06099
type: Resource
database: Pfam
comment: Phenol_hyd_sub
signature sequence match: Pfam PF06099 match 1

PIRSF000039
type: Resource
database: PIRSF
comment: Phenol_monooxy_K
signature sequence match: PIRSF PIRSF000039 match 1

SIPB779B97DBE2D1AD2
type: Structured Name
full name: Phenol 2-monooxygenase, auxiliary component DmpK

SIP5C258063F6453F82
type: Structured Name
full name: Phenol 2-monooxygenase P0 component

SIP8C6A2712A0BB7C0F
type: Structured Name
full name: Phenol hydroxylase P0 protein

pVI150
type: Plasmid

dmpK
type: Gene
alternative label: pheA1

SIP24993B045B8B4E7F
type: Function Annotation
comment: DmpK is an auxiliary protein associated with the multicomponent phenol hydroxylase DmpLMNOP and it may be involved in the post-translational incorporation of iron into the oxygenase component of the phenol hydroxylase (PubMed:8995386). Required for growth on phenol but not for in vitro phenol hydroxylase activity (PubMed:2254258, PubMed:2254259).

SIPA926BF039FF2E567
type: Subunit Annotation
comment: Homotrimer or homotetramer (PubMed:8995386). Interacts with the phenol hydroxylase components DmpL (P1 component) and DmpN (P3 component) (PubMed:8995386).

SIPCBE1A11D36AC633D
type: Mass Spectrometry Annotation
measured value: 10451
method: ESI

SIPC3BE56A4EFAD2F03
type: Disruption Phenotype Annotation
comment: Cells lacking this gene cannot grow on phenol.

SIPBFB9280C529364D6
type: Initiator Methionine Annotation
comment: Removed
range: 22572119945787694tt1tt1

22572119945787694tt1
type: Position
type: ExactPosition
position: 1
reference: MTVTNTPTPTFDQLTRYIRVRSEPEAKFVEFDFAIGHPELFVELVLPQDAFVKFCQHNRVVAMDEAMAKAVDDDMVKWRFGDVGRRLPKDPG

PRO_0000079940
type: Chain Annotation
comment: Phenol 2-monooxygenase, auxiliary component DmpK
mass: 10455
range: 22572119945787694tt2tt92

22572119945787694tt2
type: Position
type: ExactPosition
position: 2
reference: MTVTNTPTPTFDQLTRYIRVRSEPEAKFVEFDFAIGHPELFVELVLPQDAFVKFCQHNRVVAMDEAMAKAVDDDMVKWRFGDVGRRLPKDPG

22572119945787694tt92
type: Position
type: ExactPosition
position: 92
reference: MTVTNTPTPTFDQLTRYIRVRSEPEAKFVEFDFAIGHPELFVELVLPQDAFVKFCQHNRVVAMDEAMAKAVDDDMVKWRFGDVGRRLPKDPG

MTVTNTPTPTFDQLTRYIRVRSEPEAKFVEFDFAIGHPELFVELVLPQDAFVKFCQHNRVVAMDEAMAKAVDDDMVKWRFGDVGRRLPKDPG
type: Simple Sequence
modified: 1991-02-01
version: 1
mass: 10586

http://purl.uniprot.org/uniprot/P19729#attribution-E7A1CB48C427DF76824484E9BB49D5AF
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/2254258

http://purl.uniprot.org/uniprot/P19729#attribution-E06BAF8C5982F03936E71B04F9F695C8
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/2254259

http://purl.uniprot.org/uniprot/P19729#attribution-07F2D3190ABD5B1838F105A1977F1CA6
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/8995386

http://purl.uniprot.org/uniprot/P19729#attribution-EAECA9725E4E460BC14B627E888B6588
manual: true
evidence: ECO_0000303
source: http://purl.uniprot.org/citations/2254258

http://purl.uniprot.org/uniprot/P19729#attribution-DA9616B946418E639F33F60BFD375F6C
manual: true
evidence: ECO_0000305

P19730
type: Protein
reviewed: true
created: 1991-02-01
modified: 2023-05-03
version: 86
mnemonic: DMPL_PSEUF
old mnemonic: DMPL_PSEPU
old mnemonic: DMPL_PSESP
citation: http://purl.uniprot.org/citations/2254258
citation: SIPAF0C52FCB7DC8F06
citation: http://purl.uniprot.org/citations/2254259
citation: http://purl.uniprot.org/citations/8995386
citation: http://purl.uniprot.org/citations/12186554
recommended name: SIPB44BFC2E312E0755
alternative name: SIP5F9FFF69C0742230
alternative name: SIPFC8AB3EBBDBB8743
enzyme: http://purl.uniprot.org/enzyme/1.14.13.244
organism: http://purl.uniprot.org/taxonomy/79676
encoded in: pVI150
annotation: SIP3BEE63CBD53D262A
annotation: SIP2F584E043A92B325
annotation: SIP4FF97CA23A9452E1
annotation: SIPCA3DD4A5EF970D63
annotation: SIP53B5B2E0DE50D2CA
annotation: SIP1A720585FC356281
annotation: SIP634A4E013FE02C79
annotation: PRO_0000079942
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/58
classified with: http://purl.uniprot.org/keywords/503
classified with: http://purl.uniprot.org/keywords/520
classified with: http://purl.uniprot.org/keywords/614
classified with: GO_0005727
classified with: GO_0018662
classified with: GO_0046191
sequence: P19730-1

P19730-citation-2254258
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
scope: FUNCTION
scope: PATHWAY
scope: DISRUPTION PHENOTYPE

CF600
type: Strain

P19730-citation-SIPAF0C52FCB7DC8F06
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

BH
type: Strain

P19730-citation-2254259
type: Citation Statement
scope: FUNCTION
scope: CATALYTIC ACTIVITY
scope: SUBUNIT

P19730-citation-8995386
type: Citation Statement
scope: INTERACTION WITH DMPK

P19730-citation-12186554
type: Citation Statement
scope: FUNCTION
scope: ACTIVITY REGULATION
scope: SUBUNIT

cd01058
type: Resource
database: CDD
comment: AAMH_B
signature sequence match: CDD cd01058 match 1

http://purl.uniprot.org/gene3d/1.10.620.20
type: Resource
database: Gene3D
comment: Ribonucleotide Reductase, subunit A
signature sequence match: Gene3D 1.10.620.20 match 1

IPR009078
type: Resource
database: InterPro
comment: Ferritin-like_SF

IPR012078
type: Resource
database: InterPro
comment: MP_mOase_hydro

IPR003430
type: Resource
database: InterPro
comment: Phenol_Hydrox

IPR012348
type: Resource
database: InterPro
comment: RNR-like

PF02332
type: Resource
database: Pfam
comment: Phenol_Hydrox
signature sequence match: Pfam PF02332 match 1

PIRSF000040
type: Resource
database: PIRSF
comment: MMOH_comp
signature sequence match: PIRSF PIRSF000040 match 1

SSF47240
type: Resource
database: SUPFAM
comment: Ferritin-like
signature sequence match: SUPFAM SSF47240 match 1

SIPB44BFC2E312E0755
type: Structured Name
full name: Phenol 2-monooxygenase, oxygenase component DmpL
ec name: 1.14.13.244

SIP5F9FFF69C0742230
type: Structured Name
full name: Phenol 2-monooxygenase P1 component

SIPFC8AB3EBBDBB8743
type: Structured Name
full name: Phenol hydroxylase P1 protein

pVI150
type: Plasmid

dmpL
type: Gene
alternative label: pheA2

SIP3BEE63CBD53D262A
type: Function Annotation
comment: Part of a multicomponent enzyme which catalyzes the degradation of phenol and some of its methylated derivatives (PubMed:2254259). DmpL, DmpN and DmpO form the oxygenase component of the complex (PubMed:12186554). Required for growth on phenol and for in vitro phenol hydroxylase activity (PubMed:2254258, PubMed:2254259).

SIP4FF97CA23A9452E1
type: Activity Regulation Annotation
comment: Requires DmpM for efficient turnover. The activity of DmpLNO oxygenase is inhibited by dithiothreitol (DTT) by a mechanism apparently involving H(2)O(2) generation.

SIP53B5B2E0DE50D2CA
type: Subunit Annotation
comment: The multicomponent enzyme phenol hydroxylase is formed by DmpL (P1 component), DmpM (P2 component), DmpN (P3 component), DmpO (P4 component) and DmpP (P5 component) (PubMed:2254259). The oxygenase component is a dimer composed of three subunits, DmpL, DmpN and DmpO (DmpLNO) (PubMed:12186554). DmpL interacts with the auxiliary protein DmpK (P0 component) (PubMed:8995386).

SIP1A720585FC356281
type: Disruption Phenotype Annotation
comment: Cells lacking this gene cannot grow on phenol.

SIP634A4E013FE02C79
type: Similarity Annotation
comment: Belongs to the TmoE/XamoE family.

PRO_0000079942
type: Chain Annotation
comment: Phenol 2-monooxygenase, oxygenase component DmpL
mass: 38208
range: 22572119945850926tt1tt331

22572119945850926tt1
type: Position
type: ExactPosition
position: 1
reference: P19730-1

22572119945850926tt331
type: Position
type: ExactPosition
position: 331
reference: P19730-1

P19730-1
type: Simple Sequence
modified: 1991-02-01
version: 1
mass: 38208
value: MSVEIKTNTVDPIRQTYGNLQRRFGDKPASRYQEASYDIEAVTNFHYRPLWDPQHELHDPTRTAIRMTDWHKVTDPRQFYYGAYVQTRARMQEATEHAYGFCEKRELLSRLPAELQAKLLRCLVPLRHAELGANMNNSSIAGDSIAATVTQMHIYQAMDRLGMGQYLSRIGLLLDGGTGEALDQAKAYWLDDPIWQGLRRYVEDSFVIRDWFELGLAQNLVLDGLLQPLMYQRFDQWLTENGGSDVAMLTEFMRDWYGESTRWVDAMFKTVLAENDANREQVQAWLEVWEPRAYEALLPLAEEATGIAALDEVRSAFATRLQKIGLKSREE

http://purl.uniprot.org/uniprot/P19730#attribution-A59DCF55C712C29915A710504C9BB97F
creator: UniProtKB
manual: true
evidence: ECO_0000314
source: http://purl.uniprot.org/citations/2254258

http://purl.uniprot.org/uniprot/P19730#attribution-5334FADD9DB3C2F83FCFA70B661C8579
creator: UniProtKB
manual: true
evidence: ECO_0000314
source: http://purl.uniprot.org/citations/225425

http://purl.uniprot.org/uniprot/P19730#attribution-003A13815F122FA327A78C944CE2B657
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/12186554

http://purl.uniprot.org/uniprot/P19730#attribution-E7A1CB48C427DF76824484E9BB49D5AF
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/2254258

http://purl.uniprot.org/uniprot/P19730#attribution-9FDC666876D0F03E2C6C87D589D6C5CB
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/2254259

http://purl.uniprot.org/uniprot/P19730#attribution-07F2D3190ABD5B1838F105A1977F1CA6
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/8995386

http://purl.uniprot.org/uniprot/P19730#attribution-EAECA9725E4E460BC14B627E888B6588
manual: true
evidence: ECO_0000303
source: http://purl.uniprot.org/citations/2254258

http://purl.uniprot.org/uniprot/P19730#attribution-DA9616B946418E639F33F60BFD375F6C
manual: true
evidence: ECO_0000305

P19731
type: Protein
reviewed: true
created: 1991-02-01
modified: 2023-05-03
version: 95
mnemonic: DMPM_PSEUF
old mnemonic: DMPM_PSEPU
old mnemonic: DMPM_PSESP
citation: http://purl.uniprot.org/citations/2254258
citation: SIPAF0C52FCB7DC8F06
citation: http://purl.uniprot.org/citations/2254259
citation: http://purl.uniprot.org/citations/10451366
citation: http://purl.uniprot.org/citations/12186554
citation: http://purl.uniprot.org/citations/9012665
recommended name: SIP4C6F8354AF4DB202
alternative name: SIPD6FEE65239B485F9
alternative name: SIP47A3ECCF22C927FE
enzyme: http://purl.uniprot.org/enzyme/1.14.13.244
organism: http://purl.uniprot.org/taxonomy/79676
encoded in: pVI150
annotation: SIPD850B5BAAFC3A9CA
annotation: SIP147D58A794748B54
annotation: SIPEE3DF021A988B232
annotation: SIP9482CC9F8F01D18A
annotation: SIP5338F299923C4E03
annotation: SIP4D67D18C5FA39DFA
annotation: SIP0C1E5BEFA12ADB0E
annotation: SIP7077523B758D4771
annotation: PRO_0000079943
annotation: SIPC00EC0FF5C5103B8
annotation: SIPDE5AA1AF972EA627
annotation: SIPEEBD73A2EB3602C8
annotation: SIP519ABBACB67D1070
annotation: SIPC1324C0EC5185481
annotation: SIP5C86DC4B4DCA2B61
annotation: SIP94E2E661C25F3E9A
annotation: SIP5C0D9C0F780D57DC
annotation: SIP99F6D38F1AEF0469
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/2
classified with: http://purl.uniprot.org/keywords/58
classified with: http://purl.uniprot.org/keywords/503
classified with: http://purl.uniprot.org/keywords/520
classified with: http://purl.uniprot.org/keywords/614
classified with: GO_0018662
classified with: GO_0019336
sequence: MSSLVYIAFQDNDNARYVVEAIIQDNPHAVVQHHPAMIRIEAEKRLEIRRETVEENLGRAWDVQEMLVDVITIGGNVDEDDDRFVLEWKN

P19731-citation-2254258
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
scope: FUNCTION
scope: PATHWAY
scope: DISRUPTION PHENOTYPE

CF600
type: Strain

P19731-citation-SIPAF0C52FCB7DC8F06
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

BH
type: Strain

P19731-citation-2254259
type: Citation Statement
scope: FUNCTION
scope: CATALYTIC ACTIVITY
scope: SUBUNIT

P19731-citation-10451366
type: Citation Statement
scope: FUNCTION
scope: SUBUNIT
scope: MASS SPECTROMETRY

P19731-citation-12186554
type: Citation Statement
scope: FUNCTION

P19731-citation-9012665
type: Citation Statement
scope: STRUCTURE BY NMR

PDB 1HQI tt1tt90
chain: A=1-90

http://purl.uniprot.org/gene3d/3.90.56.10
type: Resource
database: Gene3D
comment: Monooxygenase component MmoB/DmpM
signature sequence match: Gene3D 3.90.56.10 match 1

IPR003454
type: Resource
database: InterPro
comment: MOase_MmoB_DmpM

IPR036889
type: Resource
database: InterPro
comment: mOase_MmoB_DmpM_sf

PF02406
type: Resource
database: Pfam
comment: MmoB_DmpM
signature sequence match: Pfam PF02406 match 1

SSF56029
type: Resource
database: SUPFAM
comment: Monooxygenase (hydroxylase) regulatory protein
signature sequence match: SUPFAM SSF56029 match 1

SIP4C6F8354AF4DB202
type: Structured Name
full name: Phenol 2-monooxygenase, stimulatory component DmpM
ec name: 1.14.13.244

SIPD6FEE65239B485F9
type: Structured Name
full name: Phenol 2-monooxygenase P2 component

SIP47A3ECCF22C927FE
type: Structured Name
full name: Phenol hydroxylase P2 protein

pVI150
type: Plasmid

dmpM
type: Gene
alternative label: pheA3

SIPD850B5BAAFC3A9CA
type: Function Annotation
comment: Part of a multicomponent enzyme which catalyzes the degradation of phenol and some of its methylated derivatives (PubMed:2254259). DmpM is a regulatory subunit that stimulates the phenol hydroxylase activity of the complex (PubMed:10451366, PubMed:12186554). The steady-state rate of phenol hydroxylase turnover is dependent on the DmpM concentration, with a maximum observed rate at about 1.5 DmpM per oxygenase monomer. Higher concentrations of DmpM inhibit phenol hydroxylase activity (PubMed:12186554). May act by altering the redox potential of the oxygenase (PubMed:12186554). Required for growth on phenol and for in vitro phenol hydroxylase activity (PubMed:2254258, PubMed:2254259).

SIP9482CC9F8F01D18A
type: Subunit Annotation
comment: Active as a monomer (PubMed:10451366). Formation of dimers inactivates the protein (PubMed:10451366). The multicomponent enzyme phenol hydroxylase is formed by DmpL (P1 component), DmpM (P2 component), DmpN (P3 component), DmpO (P4 component) and DmpP (P5 component) (PubMed:2254259).

SIP5338F299923C4E03
type: Mass Spectrometry Annotation
measured value: 10361
method: ESI

SIP4D67D18C5FA39DFA
type: Disruption Phenotype Annotation
comment: Cells lacking this gene cannot grow on phenol.

SIP0C1E5BEFA12ADB0E
type: Similarity Annotation
comment: Belongs to the TmoD/XamoD family.

SIP7077523B758D4771
type: Initiator Methionine Annotation
comment: Removed
range: 22572119945851182tt1tt1

22572119945851182tt1
type: Position
type: ExactPosition
position: 1
reference: MSSLVYIAFQDNDNARYVVEAIIQDNPHAVVQHHPAMIRIEAEKRLEIRRETVEENLGRAWDVQEMLVDVITIGGNVDEDDDRFVLEWKN

PRO_0000079943
type: Chain Annotation
comment: Phenol 2-monooxygenase, stimulatory component DmpM
mass: 10360
range: 22572119945851182tt2tt90

22572119945851182tt2
type: Position
type: ExactPosition
position: 2
reference: MSSLVYIAFQDNDNARYVVEAIIQDNPHAVVQHHPAMIRIEAEKRLEIRRETVEENLGRAWDVQEMLVDVITIGGNVDEDDDRFVLEWKN

22572119945851182tt90
type: Position
type: ExactPosition
position: 90
reference: MSSLVYIAFQDNDNARYVVEAIIQDNPHAVVQHHPAMIRIEAEKRLEIRRETVEENLGRAWDVQEMLVDVITIGGNVDEDDDRFVLEWKN

22572119945851182tt8
type: Position
type: ExactPosition
position: 8
reference: MSSLVYIAFQDNDNARYVVEAIIQDNPHAVVQHHPAMIRIEAEKRLEIRRETVEENLGRAWDVQEMLVDVITIGGNVDEDDDRFVLEWKN

22572119945851182tt10
type: Position
type: ExactPosition
position: 10
reference: MSSLVYIAFQDNDNARYVVEAIIQDNPHAVVQHHPAMIRIEAEKRLEIRRETVEENLGRAWDVQEMLVDVITIGGNVDEDDDRFVLEWKN

22572119945851182tt14
type: Position
type: ExactPosition
position: 14
reference: MSSLVYIAFQDNDNARYVVEAIIQDNPHAVVQHHPAMIRIEAEKRLEIRRETVEENLGRAWDVQEMLVDVITIGGNVDEDDDRFVLEWKN

22572119945851182tt25
type: Position
type: ExactPosition
position: 25
reference: MSSLVYIAFQDNDNARYVVEAIIQDNPHAVVQHHPAMIRIEAEKRLEIRRETVEENLGRAWDVQEMLVDVITIGGNVDEDDDRFVLEWKN

22572119945851182tt26
type: Position
type: ExactPosition
position: 26
reference: MSSLVYIAFQDNDNARYVVEAIIQDNPHAVVQHHPAMIRIEAEKRLEIRRETVEENLGRAWDVQEMLVDVITIGGNVDEDDDRFVLEWKN

22572119945851182tt28
type: Position
type: ExactPosition
position: 28
reference: MSSLVYIAFQDNDNARYVVEAIIQDNPHAVVQHHPAMIRIEAEKRLEIRRETVEENLGRAWDVQEMLVDVITIGGNVDEDDDRFVLEWKN

22572119945851182tt43
type: Position
type: ExactPosition
position: 43
reference: MSSLVYIAFQDNDNARYVVEAIIQDNPHAVVQHHPAMIRIEAEKRLEIRRETVEENLGRAWDVQEMLVDVITIGGNVDEDDDRFVLEWKN

22572119945851182tt48
type: Position
type: ExactPosition
position: 48
reference: MSSLVYIAFQDNDNARYVVEAIIQDNPHAVVQHHPAMIRIEAEKRLEIRRETVEENLGRAWDVQEMLVDVITIGGNVDEDDDRFVLEWKN

22572119945851182tt50
type: Position
type: ExactPosition
position: 50
reference: MSSLVYIAFQDNDNARYVVEAIIQDNPHAVVQHHPAMIRIEAEKRLEIRRETVEENLGRAWDVQEMLVDVITIGGNVDEDDDRFVLEWKN

22572119945851182tt58
type: Position
type: ExactPosition
position: 58
reference: MSSLVYIAFQDNDNARYVVEAIIQDNPHAVVQHHPAMIRIEAEKRLEIRRETVEENLGRAWDVQEMLVDVITIGGNVDEDDDRFVLEWKN

22572119945851182tt60
type: Position
type: ExactPosition
position: 60
reference: MSSLVYIAFQDNDNARYVVEAIIQDNPHAVVQHHPAMIRIEAEKRLEIRRETVEENLGRAWDVQEMLVDVITIGGNVDEDDDRFVLEWKN

22572119945851182tt62
type: Position
type: ExactPosition
position: 62
reference: MSSLVYIAFQDNDNARYVVEAIIQDNPHAVVQHHPAMIRIEAEKRLEIRRETVEENLGRAWDVQEMLVDVITIGGNVDEDDDRFVLEWKN

22572119945851182tt63
type: Position
type: ExactPosition
position: 63
reference: MSSLVYIAFQDNDNARYVVEAIIQDNPHAVVQHHPAMIRIEAEKRLEIRRETVEENLGRAWDVQEMLVDVITIGGNVDEDDDRFVLEWKN

22572119945851182tt68
type: Position
type: ExactPosition
position: 68
reference: MSSLVYIAFQDNDNARYVVEAIIQDNPHAVVQHHPAMIRIEAEKRLEIRRETVEENLGRAWDVQEMLVDVITIGGNVDEDDDRFVLEWKN

22572119945851182tt69
type: Position
type: ExactPosition
position: 69
reference: MSSLVYIAFQDNDNARYVVEAIIQDNPHAVVQHHPAMIRIEAEKRLEIRRETVEENLGRAWDVQEMLVDVITIGGNVDEDDDRFVLEWKN

22572119945851182tt72
type: Position
type: ExactPosition
position: 72
reference: MSSLVYIAFQDNDNARYVVEAIIQDNPHAVVQHHPAMIRIEAEKRLEIRRETVEENLGRAWDVQEMLVDVITIGGNVDEDDDRFVLEWKN

22572119945851182tt84
type: Position
type: ExactPosition
position: 84
reference: MSSLVYIAFQDNDNARYVVEAIIQDNPHAVVQHHPAMIRIEAEKRLEIRRETVEENLGRAWDVQEMLVDVITIGGNVDEDDDRFVLEWKN

22572119945851182tt88
type: Position
type: ExactPosition
position: 88
reference: MSSLVYIAFQDNDNARYVVEAIIQDNPHAVVQHHPAMIRIEAEKRLEIRRETVEENLGRAWDVQEMLVDVITIGGNVDEDDDRFVLEWKN

MSSLVYIAFQDNDNARYVVEAIIQDNPHAVVQHHPAMIRIEAEKRLEIRRETVEENLGRAWDVQEMLVDVITIGGNVDEDDDRFVLEWKN
type: Simple Sequence
modified: 1991-02-01
version: 1
mass: 10491

http://purl.uniprot.org/uniprot/P19731#attribution-6A06DCE7A96DC7A0DC7960B041F107E1
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/10451366

http://purl.uniprot.org/uniprot/P19731#attribution-003A13815F122FA327A78C944CE2B657
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/12186554

http://purl.uniprot.org/uniprot/P19731#attribution-E7A1CB48C427DF76824484E9BB49D5AF
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/2254258

http://purl.uniprot.org/uniprot/P19731#attribution-9FDC666876D0F03E2C6C87D589D6C5CB
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/2254259

http://purl.uniprot.org/uniprot/P19731#attribution-EAECA9725E4E460BC14B627E888B6588
manual: true
evidence: ECO_0000303
source: http://purl.uniprot.org/citations/2254258

http://purl.uniprot.org/uniprot/P19731#attribution-DA9616B946418E639F33F60BFD375F6C
manual: true
evidence: ECO_0000305

P19732
type: Protein
reviewed: true
created: 1991-02-01
modified: 2023-05-03
version: 85
mnemonic: DMPN_PSEUF
old mnemonic: DMPN_PSEPU
old mnemonic: DMPN_PSESP
citation: http://purl.uniprot.org/citations/2254258
citation: SIPAF0C52FCB7DC8F06
citation: http://purl.uniprot.org/citations/2254259
citation: http://purl.uniprot.org/citations/8995386
citation: http://purl.uniprot.org/citations/12186554
recommended name: SIP97047AAE3676F1F4
alternative name: SIP6DD3D4EB227E1EC8
alternative name: SIP944C84C93444EFC0
enzyme: http://purl.uniprot.org/enzyme/1.14.13.244
organism: http://purl.uniprot.org/taxonomy/79676
encoded in: pVI150
annotation: SIPBA22657CAF24374D
annotation: SIP32DA4BD27BD7A062
annotation: SIPA3CD4938E91192CD
annotation: SIP7CC76701A9A8E04A
annotation: SIP1EB4D303085D4725
annotation: SIPB8D292C011AC2068
annotation: SIPE59C058C47AD1AA3
annotation: SIP495A09443387DD7C
annotation: PRO_0000079945
annotation: SIPB7D2AE229C305446
annotation: SIPF6B8E67AC2591646
annotation: SIPCD619CEAF66B6F0F
annotation: SIP3846CCD636EC5B93
annotation: SIP7D0362AD7AD12D47
annotation: SIPB302E6C722F64B06
annotation: SIPAD0F31E2F3DE7356
annotation: SIP1DCE7223C8050FC3
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/58
classified with: http://purl.uniprot.org/keywords/408
classified with: http://purl.uniprot.org/keywords/479
classified with: http://purl.uniprot.org/keywords/503
classified with: http://purl.uniprot.org/keywords/520
classified with: http://purl.uniprot.org/keywords/614
classified with: GO_0046872
classified with: GO_0018662
classified with: GO_0019336
sequence: P19732-1

P19732-citation-2254258
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
scope: FUNCTION
scope: PATHWAY
scope: DISRUPTION PHENOTYPE

CF600
type: Strain

P19732-citation-SIPAF0C52FCB7DC8F06
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

BH
type: Strain

P19732-citation-2254259
type: Citation Statement
scope: FUNCTION
scope: CATALYTIC ACTIVITY
scope: SUBUNIT

P19732-citation-8995386
type: Citation Statement
scope: INTERACTION WITH DMPK

P19732-citation-12186554
type: Citation Statement
scope: FUNCTION
scope: COFACTOR
scope: ACTIVITY REGULATION
scope: SUBUNIT

cd01057
type: Resource
database: CDD
comment: AAMH_A
signature sequence match: CDD cd01057 match 1

IPR007029
type: Resource
database: InterPro
comment: YHS_dom

PF04945
type: Resource
database: Pfam
comment: YHS
signature sequence match: Pfam PF04945 match 1

SIP97047AAE3676F1F4
type: Structured Name
full name: Phenol 2-monooxygenase, oxygenase component DmpN
ec name: 1.14.13.244

SIP6DD3D4EB227E1EC8
type: Structured Name
full name: Phenol 2-monooxygenase P3 component

SIP944C84C93444EFC0
type: Structured Name
full name: Phenol hydroxylase P3 protein

pVI150
type: Plasmid

dmpN
type: Gene
alternative label: pheA4

SIPBA22657CAF24374D
type: Function Annotation
comment: Part of a multicomponent enzyme which catalyzes the degradation of phenol and some of its methylated derivatives (PubMed:2254259). DmpL, DmpN and DmpO form the oxygenase component of the complex (PubMed:12186554). Required for growth on phenol and for in vitro phenol hydroxylase activity (PubMed:2254258, PubMed:2254259).

SIPA3CD4938E91192CD
type: Cofactor Annotation
comment: Binds 2 Fe(2+) ions per subunit.
cofactor: CHEBI_29033

SIP7CC76701A9A8E04A
type: Activity Regulation Annotation
comment: Requires DmpM for efficient turnover. The activity of DmpLNO oxygenase is inhibited by dithiothreitol (DTT) by a mechanism apparently involving H(2)O(2) generation.

SIPB8D292C011AC2068
type: Subunit Annotation
comment: The multicomponent enzyme phenol hydroxylase is formed by DmpL (P1 component), DmpM (P2 component), DmpN (P3 component), DmpO (P4 component) and DmpP (P5 component) (PubMed:2254259). The oxygenase component is a dimer composed of three subunits, DmpL, DmpN and DmpO (DmpLNO) (PubMed:12186554). DmpN interacts with the auxiliary protein DmpK (P0 component) (PubMed:8995386).

SIPE59C058C47AD1AA3
type: Disruption Phenotype Annotation
comment: Cells lacking this gene cannot grow on phenol.

SIP495A09443387DD7C
type: Similarity Annotation
comment: Belongs to the TmoA/XamoA family.

PRO_0000079945
type: Chain Annotation
comment: Phenol 2-monooxygenase, oxygenase component DmpN
mass: 60523
range: 22572119945851438tt1tt517

22572119945851438tt1
type: Position
type: ExactPosition
position: 1
reference: P19732-1

22572119945851438tt517
type: Position
type: ExactPosition
position: 517
reference: P19732-1

22572119945851438tt109
type: Position
type: ExactPosition
position: 109
reference: P19732-1

22572119945851438tt139
type: Position
type: ExactPosition
position: 139
reference: P19732-1

22572119945851438tt142
type: Position
type: ExactPosition
position: 142
reference: P19732-1

22572119945851438tt200
type: Position
type: ExactPosition
position: 200
reference: P19732-1

22572119945851438tt234
type: Position
type: ExactPosition
position: 234
reference: P19732-1

22572119945851438tt237
type: Position
type: ExactPosition
position: 237
reference: P19732-1

1
subclass of: CHEBI_24875
comment: catalytic

2
subclass of: CHEBI_24875
comment: catalytic

P19732-1
type: Simple Sequence
modified: 1991-02-01
version: 1
mass: 60523
value: MATHNKKRLNLKDKYRYLTRDLAWETTYQKKEDVFPLEHFEGIKITDWDKWEDPFRLTMDTYWKYQAEKEKKLYAIFDAFAQNNGHQNISDARYVNALKLFLTAVSPLEYQAFQGFSRVGRQFSGAGARVACQMQAIDELRHVQTQVHAMSHYNKHFDGLHDFAHMYDRVWYLSVPKSYMDDARTAGPFEFLTAVSFSFEYVLTNLLFVPFMSGAAYNGDMATVTFGFSAQSDEARHMTLGLEVIKFMLEQHEDNVPIIQRWIDKWFWRGYRLLTLIGMMMDYMLPNKVMSWSEAWGVYFEQAGGALFKDLERYGIRPPKYVEQTTIGKEHITHQVWGALYQYSKATSFHTWIPGDEELNWLSEKYPDTFDKYYRPRFEFWREQQAKGERFYNDTLPHLCQVCQLPVIFTEPDDPTKLSLRSLVHEGERYQFCSDGCCDIFKNEPVKYIQAWLPVHQIYQGNCEGGDVETVVQKYYHIKSGVDNLEYLGSPEHQRWLALKGQTPPTAAPADKSLGAA

http://purl.uniprot.org/uniprot/P19732#attribution-0068A75D15C6A9186487E8C1A7C3968B
manual: true
evidence: ECO_0000250
source: Q00456

http://purl.uniprot.org/uniprot/P19732#attribution-003A13815F122FA327A78C944CE2B657
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/12186554

http://purl.uniprot.org/uniprot/P19732#attribution-E7A1CB48C427DF76824484E9BB49D5AF
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/2254258

http://purl.uniprot.org/uniprot/P19732#attribution-9FDC666876D0F03E2C6C87D589D6C5CB
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/2254259

http://purl.uniprot.org/uniprot/P19732#attribution-07F2D3190ABD5B1838F105A1977F1CA6
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/8995386

http://purl.uniprot.org/uniprot/P19732#attribution-EAECA9725E4E460BC14B627E888B6588
manual: true
evidence: ECO_0000303
source: http://purl.uniprot.org/citations/2254258

http://purl.uniprot.org/uniprot/P19732#attribution-DA9616B946418E639F33F60BFD375F6C
manual: true
evidence: ECO_0000305

P19733
type: Protein
reviewed: true
created: 1991-02-01
modified: 2023-05-03
version: 70
mnemonic: DMPO_PSEUF
old mnemonic: DMPO_PSEPU
old mnemonic: DMPO_PSESP
citation: http://purl.uniprot.org/citations/2254258
citation: SIPAF0C52FCB7DC8F06
citation: http://purl.uniprot.org/citations/2254259
citation: http://purl.uniprot.org/citations/12186554
recommended name: SIPF9452319E7F7716D
alternative name: SIP21BAF447F2BA0ADF
alternative name: SIP4485719B7FA18D9B
enzyme: http://purl.uniprot.org/enzyme/1.14.13.244
organism: http://purl.uniprot.org/taxonomy/79676
encoded in: pVI150
annotation: SIP18768C7B0D8F39F6
annotation: SIP2B0FF55D0F7D19E4
annotation: SIPC00D490DBEBB0866
annotation: SIP8C85EE3F920F6C7A
annotation: SIPD18290192105E99A
annotation: SIPCDFFEF55997E3677
annotation: PRO_0000079946
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/58
classified with: http://purl.uniprot.org/keywords/503
classified with: http://purl.uniprot.org/keywords/520
classified with: http://purl.uniprot.org/keywords/614
classified with: GO_0018662
classified with: GO_0019336
sequence: P19733-1

P19733-citation-2254258
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
scope: FUNCTION
scope: PATHWAY
scope: DISRUPTION PHENOTYPE

CF600
type: Strain

P19733-citation-SIPAF0C52FCB7DC8F06
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

BH
type: Strain

P19733-citation-2254259
type: Citation Statement
scope: FUNCTION
scope: CATALYTIC ACTIVITY
scope: SUBUNIT

P19733-citation-12186554
type: Citation Statement
scope: FUNCTION
scope: ACTIVITY REGULATION
scope: SUBUNIT

http://purl.uniprot.org/gene3d/3.10.20.560
type: Resource
database: Gene3D
comment: Phenol hydroxylase
signature sequence match: Gene3D 3.10.20.560 match 1

IPR006756
type: Resource
database: InterPro
comment: Phenol_hydroxylase

IPR043010
type: Resource
database: InterPro
comment: Phenol_hydroxylase_sf

PF04663
type: Resource
database: Pfam
comment: Phenol_monoox
signature sequence match: Pfam PF04663 match 1

SIPF9452319E7F7716D
type: Structured Name
full name: Phenol 2-monooxygenase, oxygenase component DmpO
ec name: 1.14.13.244

SIP21BAF447F2BA0ADF
type: Structured Name
full name: Phenol 2-monooxygenase P4 component

SIP4485719B7FA18D9B
type: Structured Name
full name: Phenol hydroxylase P4 protein

pVI150
type: Plasmid