NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
D-amino Acid Aminotransferase, subunit A, domain 2
BRANCHED-CHAIN-AMINO-ACID AMINOTRANSFERASE
D-aminoacid aminotransferase-like PLP-dependent enzymes
Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine.
Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.
YVTN repeat-like/Quinoprotein amine dehydrogenase
RIBOSOME BIOGENESIS PROTEIN BOP1 BLOCK OF PROLIFERATION 1 PROTEIN
Ribosome biogenesis protein BOP1 homolog
Required for maturation of ribosomal RNAs and formation of the large ribosomal subunit.
Belongs to the WD repeat BOP1/ERB1 family.
Required for processing of 20S pre-rRNA precursor and biogenesis of 40S ribosomal subunits.
Coiled-coil domain-containing protein 42 homolog
CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED
Probable cytosolic iron-sulfur protein assembly protein CIAO1 homolog
Essential component of the cytosolic iron-sulfur (Fe/S) protein assembly machinery. Required for the maturation of extramitochondrial Fe/S proteins.
Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
S-adenosyl-L-methionine-dependent methyltransferases
Fe-S cluster assembly protein DRE2 homolog
Component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery. Required for the maturation of extramitochondrial Fe-S proteins. Part of an electron transfer chain functioning in an early step of cytosolic Fe-S biogenesis, facilitating the de novo assembly of a [4Fe-4S] cluster on the cytosolic Fe-S scaffold complex. Electrons are transferred from NADPH via a FAD- and FMN-containing diflavin oxidoreductase. Together with the diflavin oxidoreductase, also required for the assembly of the diferric tyrosyl radical cofactor of ribonucleotide reductase (RNR), probably by providing electrons for reduction during radical cofactor maturation in the catalytic small subunit.
The C-terminal domain binds 2 Fe-S clusters but is otherwise mostly in an intrinsically disordered conformation.
The N-terminal domain has structural similarity with S-adenosyl-L-methionine-dependent methyltransferases, but does not bind S-adenosyl-L-methionine. It is required for correct assembly of the 2 Fe-S clusters.
The twin Cx2C motifs are involved in the recognition by the mitochondrial MIA40-ERV1 disulfide relay system. The formation of 2 disulfide bonds in the Cx2C motifs through dithiol/disulfide exchange reactions effectively traps the protein in the mitochondrial intermembrane space.
EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT A
EUKARYOTIC TRANSLATION INITIATION FACTOR 3, THETA SUBUNIT
Eukaryotic translation initiation factor 3 subunit 10
RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex.
EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT E
Eukaryotic translation initiation factor 3 subunit 6
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.
EUKARYOTIC TRANSLATION INITIATION FACTOR 3 -RELATED
EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT H
EUKARYOTIC TRANSLATION INITIATION FACTOR 3
EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT L
2,3-diketo-5-methylthio-1-phosphopentane phosphatase
Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene).
Belongs to the HAD-like hydrolase superfamily. MasA/MtnC family.
5' to 3' exonuclease, C-terminal subdomain
Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.
Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.
Interacts with PCNA. Three molecules of FEN1 bind to one PCNA trimer with each molecule binding to one PCNA monomer. PCNA stimulates the nuclease activity without altering cleavage specificity.
Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.
Phosphorylated. Phosphorylation upon DNA damage induces relocalization to the nuclear plasma.
Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.
Solute carrier family 35 member C1 homolog
Antiporter specific for GDP-l-fucose and depending on the concomitant reverse transport of GMP. Involved in GDP-fucose import from the cytoplasm into the Golgi lumen.
Belongs to the TPT transporter family. SLC35C subfamily.
Belongs to the glycoside hydrolase-like 3 (GHL3) family.
INOSINE TRIPHOSPHATE PYROPHOSPHATASE/HAM1 PROTEIN
Non-canonical purine NTP pyrophosphatase
Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as inosine triphosphate (ITP), deoxyinosine triphosphate (dITP) or xanthosine 5'-triphosphate (XTP) to their respective monophosphate derivatives. The enzyme does not distinguish between the deoxy- and ribose forms. Probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions.
Binds 1 divalent metal cation per subunit; can use either Mg(2+) or Mn(2+).
Type I PLP-dependent aspartate aminotransferase-like (Major domain)
Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3-hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3-hydroxyanthranilic acid (3-OHAA), respectively.
WD_REPEATS_REGION DOMAIN-CONTAINING PROTEIN
Lissencephaly-1 protein (Lis-1, PAF-AH alpha) N-terminal domain
Positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus end. Required for several dynein- and microtubule-dependent processes.
Localizes to the plus end of microtubules and to the centrosome.
Dimerization mediated by the LisH domain may be required to activate dynein.
Belongs to the WD repeat LIS1/nudF family.
translation initiation factor eif-2b, domain 1
S-methyl-5-thioribose-1-phosphate isomerase
Translation initiation factor eIF-2B subunit alpha/beta/delta-like protein
Catalyzes the interconversion of methylthioribose-1-phosphate (MTR-1-P) into methylthioribulose-1-phosphate (MTRu-1-P).
Belongs to the eIF-2B alpha/beta/delta subunits family. MtnA subfamily.
Class II aldolase/adducin N-terminal domain
METHYLTHIORIBULOSE-1-PHOSPHATE DEHYDRATASE
Probable methylthioribulose-1-phosphate dehydratase
Catalyzes the dehydration of methylthioribulose-1-phosphate (MTRu-1-P) into 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P).
Belongs to the aldolase class II family. MtnB subfamily.
1,2-DIHYDROXY-3-KETO-5-METHYLTHIOPENTENE DIOXYGENASE
Acireductone dioxygenase (Fe(2+)-requiring)
Acireductone dioxygenase (Ni(2+)-requiring)
Catalyzes 2 different reactions between oxygen and the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) depending upon the metal bound in the active site. Fe-containing acireductone dioxygenase (Fe-ARD) produces formate and 2-keto-4-methylthiobutyrate (KMTB), the alpha-ketoacid precursor of methionine in the methionine recycle pathway. Ni-containing acireductone dioxygenase (Ni-ARD) produces methylthiopropionate, carbon monoxide and formate, and does not lie on the methionine recycle pathway.
Binds either 1 Fe or Ni cation per monomer. Iron-binding promotes an acireductone dioxygenase reaction producing 2-keto-4-methylthiobutyrate, while nickel-binding promotes an acireductone dioxygenase reaction producing 3-(methylsulfanyl)propanoate.
Belongs to the acireductone dioxygenase (ARD) family.
for iron-dependent acireductone dioxygenase activity
for nickel-dependent acireductone dioxygenase activity
Nitrile hydratase alpha /Thiocyanate hydrolase gamma
NHase catalyzes the hydration of various nitrile compounds to the corresponding amides. Industrial production of acrylamide is now being developed using some of the enzymes of this class (By similarity).
Oxidation on Cys-403 is essential for the activity.
Oxidation on Cys-405 stabilizes the Fe-NO ligand coordinated in the inactive form.
In the N-terminal section; belongs to the nitrile hydratase subunit alpha family.
In the C-terminal section; belongs to the nitrile hydratase subunit beta family.
P-loop containing nucleotide triphosphate hydrolases
CYTOSOLIC FE-S CLUSTER ASSEMBLY FACTOR NUBP2
NUCLEOTIDE-BINDING PROTEIN NBP35 YEAST -RELATED
P-loop containing nucleoside triphosphate hydrolases
Cytosolic Fe-S cluster assembly factor NUBP2 homolog
Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The NUBP1-NUBP2 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins.
Binds 4 [4Fe-4S] clusters per heterotetramer. Contains two stable clusters in the N-termini of NUBP1 and two labile, bridging clusters between subunits of the NUBP1-NUBP2 heterotetramer.
Heterotetramer of 2 NUBP1 and 2 NUBP2 chains.
Belongs to the Mrp/NBP35 ATP-binding proteins family. NUBP2/CFD1 subfamily.
Adenylosuccinate Synthetase, subunit A, domain 1
Adenylosuccinate Synthetase, subunit A, domain 2
Adenylosuccinate Synthetase, subunit A, domain 3
Plays an important role in the de novo pathway and in the salvage pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP (By similarity).
Belongs to the adenylosuccinate synthetase family.
Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains about 33 different proteins and 1 molecule of RNA (18S). The 60S subunit contains about 49 different proteins and 3 molecules of RNA (25S, 5.8S and 5S).
Belongs to the eukaryotic ribosomal protein eS1 family.
Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits.
Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains about 33 different proteins and 1 molecule of RNA (18S). The 60S subunit contains about 49 different proteins and 3 molecules of RNA (25S, 5.8S and 5S). Interacts with ribosomal protein S21.
Belongs to the universal ribosomal protein uS2 family.
3'-phosphate/5'-hydroxy nucleic acid ligase
Catalytic subunit of the tRNA-splicing ligase complex that acts by directly joining spliced tRNA halves to mature-sized tRNAs by incorporating the precursor-derived splice junction phosphate into the mature tRNA as a canonical 3',5'-phosphodiester. May act as an RNA ligase with broad substrate specificity, and may function toward other RNAs.
Catalytic component of the tRNA-splicing ligase complex.
Ligation probably proceeds through 3 nucleotidyl transfer steps, with 2',3'-cyclic phosphate termini being hydrolyzed to 3'-P termini in a step that precedes 3'-P activation with GMP. In the first nucleotidyl transfer step, RTCB reacts with GTP to form a covalent RTCB-histidine-GMP intermediate with release of PPi; in the second step, the GMP moiety is transferred to the RNA 3'-P; in the third step, the 5'-OH from the opposite RNA strand attacks the activated 3'-P to form a 3',5'-phosphodiester bond and release GMP.
Enhances the incorporation of serine into phosphatidylserine and sphingolipids.
NAD-dependent protein deacylase. Catalyzes the NAD-dependent hydrolysis of acyl groups from lysine residues.
Belongs to the sirtuin family. Class II subfamily.
Zinc/RING finger domain, C3HC4 (zinc finger)
STRUCTURE-SPECIFIC ENDONUCLEASE SUBUNIT SLX1
Structure-specific endonuclease subunit SLX1 homolog
Catalytic subunit of a heterodimeric structure-specific endonuclease that resolves DNA secondary structures generated during DNA repair and recombination. Has endonuclease activity towards branched DNA substrates, introducing single-strand cuts in duplex DNA close to junctions with ss-DNA.
Forms a heterodimer with a member of the SLX4 family.
Probably involved in the biogenesis of the COX complex.
UBIQUITIN-FOLD MODIFIER-CONJUGATING ENZYME 1
E2-like enzyme which forms an intermediate with UFM1 via a thioester linkage.
Belongs to the ubiquitin-conjugating enzyme family. UFC1 subfamily.
Ribosome biogenesis protein WDR12 homolog
Belongs to the WD repeat WDR12/YTM1 family.