http://purl.uniprot.org/uniprot/A0A482D308
type: Protein
reviewed: true
created: 2023-05-03
modified: 2023-09-13
version: 11
mnemonic: CS12F_UNCAX
citation: http://purl.uniprot.org/citations/30337455
citation: http://purl.uniprot.org/citations/31857715
citation: http://purl.uniprot.org/citations/32246713
citation: http://purl.uniprot.org/citations/33333018
recommended name: SIP9450C49E221B5D94
alternative name: SIP1618F1A50B94B15C
enzyme: http://purl.uniprot.org/enzyme/3.1.-.-
organism: http://purl.uniprot.org/taxonomy/115547
annotation: SIPD2A5D55D581D43B9
annotation: SIP5A8395BB790007BB
annotation: SIP5D1CE3B553EF5009
annotation: SIPB6682EF14B5F3D34
annotation: SIP8C98AC052F3635C6
annotation: SIPCBE08E83D611F860
annotation: SIP9708C98D5AD3819A
annotation: SIP9F20172E444061C6
annotation: SIPBCD2B014B619F0A0
annotation: SIPDC0B624B79E54E19
annotation: PRO_0000457914
annotation: SIP8953389DD23D8D8D
annotation: SIP746F5605A920BF90
annotation: SIP985C49C9DD21526F
annotation: SIP74E9F224212E3ABD
annotation: SIP4820427DE6572BDB
annotation: SIP0B392877F302ED3E
annotation: SIP7E413A5A205050F8
annotation: SIPB840C98269C2F163
annotation: SIPE6BE43D4CC359EFC
annotation: SIPC2C3DB68F395A780
annotation: SIPDAC93B0AE35C2C46
annotation: SIP2D64E61CEC4EA437
annotation: SIPD381AEFBA24CA3B4
annotation: SIP3FCB49C56E3820DE
annotation: SIP9D0FDB24E3B075E0
annotation: SIP07B5C3FEB6C2E470
annotation: SIP7EE72529358D578E
annotation: SIP7A2A97BCB9F543B3
annotation: SIP45CA301CA6A10C78
annotation: SIP46D25C170EA7D39E
annotation: SIP76943DAC08C48B07
annotation: SIPA381C8A22B753B80
annotation: SIP11E9115B8CEAA3C0
annotation: SIP4803630EF702F23A
annotation: SIPE51D6BAD7755CBD4
annotation: SIP5833B5B2F6F79FAA
annotation: SIP5E6F4841DBEA1A5D
annotation: SIPC83430F6C6A0DD07
annotation: SIPA98DB366538951BA
annotation: SIP70930C4B5827C784
annotation: SIP1A28A5B354BB419F
annotation: SIP698A046346126F94
annotation: SIP791D1140EB885A8A
annotation: SIP69B1E558A82FF4BB
annotation: SIP2054C9F798C7C535
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/2
classified with: http://purl.uniprot.org/keywords/51
classified with: http://purl.uniprot.org/keywords/238
classified with: http://purl.uniprot.org/keywords/255
classified with: http://purl.uniprot.org/keywords/479
classified with: http://purl.uniprot.org/keywords/694
classified with: http://purl.uniprot.org/keywords/862
classified with: GO_0003677
classified with: GO_0004519
classified with: GO_0046872
classified with: GO_0003723
classified with: GO_0051607
sequence: http://purl.uniprot.org/isoforms/A0A482D308-1

A0A482D308-citation-30337455
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
scope: FUNCTION
scope: CATALYTIC ACTIVITY
scope: PROBABLE ACTIVE SITES
scope: COFACTOR
scope: ACTIVITY REGULATION
scope: BIOPHYSICOCHEMICAL PROPERTIES
scope: BIOTECHNOLOGY
scope: DNA-BINDING
scope: RNA-BINDING
scope: MUTAGENESIS OF ASP-326; GLU-422; ARG-490 AND ASP-510

A0A482D308-citation-31857715
type: Citation Statement
scope: NOMENCLATURE

A0A482D308-citation-32246713
type: Citation Statement
scope: FUNCTION
scope: CATALYTIC ACTIVITY
scope: PROBABLE ACTIVE SITES
scope: COFACTOR
scope: ACTIVITY REGULATION
scope: BIOPHYSICOCHEMICAL PROPERTIES
scope: RNA-BINDING
scope: MUTAGENESIS OF ASP-326 AND ASP-510

A0A482D308-citation-33333018
type: Citation Statement
scope: STRUCTURE BY ELECTRON MICROSCOPY (3.00 ANGSTROMS) IN COMPLEX WITH GUIDE RNA; TARGET DNA AND ZINC
scope: FUNCTION
scope: REACTION MECHANISM
scope: CATALYTIC ACTIVITY
scope: COFACTOR
scope: ACTIVITY REGULATION
scope: SUBUNIT
scope: DOMAIN
scope: MUTAGENESIS OF 39-LEU--CYS-72; 118-ILE--ILE-126; ILE-118; TYR-122; ILE-126; TYR-146; MET-178; GLN-197; LYS-198; SER-286; ASP-326; 366-LEU--LYS-383 AND PHE-487

7C7L
type: Structure Resource
database: PDB
method: Electron Microscopy
resolution: 3.00
chain sequence mapping: PDB 7C7L tt1tt529

PDB 7C7L tt1tt529
chain: A/B=1-529

IPR010095
type: Resource
database: InterPro
comment: Cas12f1-like_TNB

NF040570
type: Resource
database: NCBIfam
comment: guided_TnpB
signature sequence match: NCBIfam NF040570 match 1

TIGR01766
type: Resource
database: NCBIfam
comment: IS200/IS605 family accessory protein TnpB-like domain
signature sequence match: NCBIfam TIGR01766 match 1

PF07282
type: Resource
database: Pfam
comment: OrfB_Zn_ribbon
signature sequence match: Pfam PF07282 match 1

SIP9450C49E221B5D94
type: Structured Name
full name: CRISPR-associated endodeoxyribonuclease Cas12f1
short name: Un1Cas12f1
ec name: 3.1.-.-

SIP1618F1A50B94B15C
type: Structured Name
full name: CRISPR-associated endodeoxyribonuclease Cas14a1

cas12f
type: Gene
alternative label: cas14a1

SIPD2A5D55D581D43B9
type: Function Annotation
comment: CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids (Probable). CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA), which requires a trans-encoded small RNA (tracrRNA), but not this protein (in vitro) (Probable). Upon expression in E.coli of this protein, a mini CRISPR array and the probable tracrRNA, the protein associates with both RNAs (PubMed:30337455). The mini system is not active in E.coli against phiX174 phage, nor is it active in protection against transformation by foreign plasmids (PubMed:30337455, PubMed:32246713). In vitro the purified protein-tracrRNA-crRNA complex cleaves ssDNA complementary to the crRNA; target cleavage requires both tracrRNA and crRNA, but not a protospacer adjacent motif (PAM). The tracrRNA-crRNA can be replaced by a single guide RNA (sgRNA). 2-nucleotide mismatches in the middle of the crRNA:DNA heteroduplex decrease cleavage. Cleavage occurs just downstream of the heteroduplex (PubMed:30337455). Activation of this protein results in non-specific ssDNA degradation in vitro (PubMed:30337455, PubMed:32246713). In vitro and in E.coli (coexpressed with sgRNA) has dsDNA endonuclease activity, recognizing the 5' PAM sequence TTTR; both sgRNA and a PAM are required for activity. Cleaves the target strand 24 and the nontarget strand 22 bases upstream of the PAM (respectively), resulting in 5' overhangs (PubMed:32246713, PubMed:33333018). The 2 monomers interact differently with the sgRNA and target DNA. Mutagenesis of a dimeric construct shows that one of the RuvC monomers probably cleaves both DNA strands (PubMed:33333018).

SIP5A8395BB790007BB
type: Cofactor Annotation
comment: Mg(2+) is required for dsDNA cleavage (PubMed:32246713). Mg(2+) and Mn(2+) support ssDNA cleavage equally (PubMed:30337455).
cofactor: CHEBI_18420

SIP5D1CE3B553EF5009
type: Cofactor Annotation
comment: Homodimeric Cas12f in complex with sgRNA and target DNA binds only 3 Zn(2+) ions.
cofactor: CHEBI_29105

SIPB6682EF14B5F3D34
type: Activity Regulation Annotation
comment: Target ssDNA cleavage is inhibited by EDTA (PubMed:30337455). Activity is maximal with 5-50 mM NaCl, is less efficient at higher NaCl concentrations (PubMed:32246713, PubMed:33333018).

SIP8C98AC052F3635C6
type: Temperature Dependence Annotation
comment: Optimum temperature for dsDNA cleavage is 46 degrees Celsius and occurs between 37-52 degrees Celsius, no cleavage at 55 degrees Celsius (PubMed:32246713). Optimum temperature for ssDNA cleavage is 45-55 degrees Celsius (PubMed:30337455).

SIPCBE08E83D611F860
type: Subunit Annotation
comment: An asymmetric homodimer. Guide RNA is probably required for dimerization.

SIP9708C98D5AD3819A
type: Domain Annotation
comment: Has an asymmetric bilobed structure, with a recognition (REC) lobe and nuclease (NUC) lobe; the sgRNA:target DNA is bound between the 2 lobes. The REC lobe (residues 1-312) is formed by the WED, ZF and REC domains, while the NUC lobe (residues 321-529) is formed by the RuvC and TNB domains. Has a split RuvC-like domain with the nuclease active sites.

SIP9F20172E444061C6
type: Biotechnology Annotation
comment: The requirement for a perfect match in the middle of the heteroduplex, joined with its lack of a PAM, allows its use in detection of single-nucleotide polymorphisms (a DETECTR assay). The tracrRNA-crRNA can be replaced by a single guide RNA (sgRNA) (PubMed:30337455). Its inability to protect against plasmid transformation suggests there are elements missing in E.coli which prevents its use in more classic genome editing (PubMed:32246713).

SIPBCD2B014B619F0A0
type: Annotation
comment: Part of a type V-F CRISPR-Cas system.

SIPDC0B624B79E54E19
type: Similarity Annotation
comment: Belongs to the CRISPR-associated endonuclease Cas12f family.

PRO_0000457914
type: Chain Annotation
comment: CRISPR-associated endodeoxyribonuclease Cas12f1
mass: 61517
range: -9218568810373570514tt1tt529

-9218568810373570514tt1
type: Position
type: ExactPosition
position: 1
reference: http://purl.uniprot.org/isoforms/A0A482D308-1

-9218568810373570514tt529
type: Position
type: ExactPosition
position: 529
reference: http://purl.uniprot.org/isoforms/A0A482D308-1

SIP8953389DD23D8D8D
type: Region Annotation
comment: Zinc finger domain (ZF)
range: -9218568810373570514tt1tt95

-9218568810373570514tt95
type: Position
type: ExactPosition
position: 95
reference: http://purl.uniprot.org/isoforms/A0A482D308-1

SIP746F5605A920BF90
type: Region Annotation
comment: Recognition domain (REC)
range: -9218568810373570514tt96tt192

-9218568810373570514tt96
type: Position
type: ExactPosition
position: 96
reference: http://purl.uniprot.org/isoforms/A0A482D308-1

-9218568810373570514tt192
type: Position
type: ExactPosition
position: 192
reference: http://purl.uniprot.org/isoforms/A0A482D308-1

SIP985C49C9DD21526F
type: Region Annotation
comment: Wedge domain (WED)
range: -9218568810373570514tt193tt312

-9218568810373570514tt193
type: Position
type: ExactPosition
position: 193
reference: http://purl.uniprot.org/isoforms/A0A482D308-1

-9218568810373570514tt312
type: Position
type: ExactPosition
position: 312
reference: http://purl.uniprot.org/isoforms/A0A482D308-1

SIP74E9F224212E3ABD
type: Region Annotation
comment: Linker
range: -9218568810373570514tt313tt321

-9218568810373570514tt313
type: Position
type: ExactPosition
position: 313
reference: http://purl.uniprot.org/isoforms/A0A482D308-1

-9218568810373570514tt321
type: Position
type: ExactPosition
position: 321
reference: http://purl.uniprot.org/isoforms/A0A482D308-1

SIP4820427DE6572BDB
type: Region Annotation
comment: RuvC-I
range: -9218568810373570514tt322tt473

-9218568810373570514tt322
type: Position
type: ExactPosition
position: 322
reference: http://purl.uniprot.org/isoforms/A0A482D308-1

-9218568810373570514tt473
type: Position
type: ExactPosition
position: 473
reference: http://purl.uniprot.org/isoforms/A0A482D308-1

SIP0B392877F302ED3E
type: Region Annotation
comment: Target nucleic acid-binding (TNB)
range: -9218568810373570514tt474tt508

-9218568810373570514tt474
type: Position
type: ExactPosition
position: 474
reference: http://purl.uniprot.org/isoforms/A0A482D308-1

-9218568810373570514tt508
type: Position
type: ExactPosition
position: 508
reference: http://purl.uniprot.org/isoforms/A0A482D308-1

SIP7E413A5A205050F8
type: Region Annotation
comment: RuvC-II
range: -9218568810373570514tt509tt529

-9218568810373570514tt509
type: Position
type: ExactPosition
position: 509
reference: http://purl.uniprot.org/isoforms/A0A482D308-1

-9218568810373570514tt326
type: Position
type: ExactPosition
position: 326
reference: http://purl.uniprot.org/isoforms/A0A482D308-1

-9218568810373570514tt422
type: Position
type: ExactPosition
position: 422
reference: http://purl.uniprot.org/isoforms/A0A482D308-1

-9218568810373570514tt490
type: Position
type: ExactPosition
position: 490
reference: http://purl.uniprot.org/isoforms/A0A482D308-1

-9218568810373570514tt510
type: Position
type: ExactPosition
position: 510
reference: http://purl.uniprot.org/isoforms/A0A482D308-1

-9218568810373570514tt50
type: Position
type: ExactPosition
position: 50
reference: http://purl.uniprot.org/isoforms/A0A482D308-1

-9218568810373570514tt53
type: Position
type: ExactPosition
position: 53
reference: http://purl.uniprot.org/isoforms/A0A482D308-1

-9218568810373570514tt69
type: Position
type: ExactPosition
position: 69
reference: http://purl.uniprot.org/isoforms/A0A482D308-1

-9218568810373570514tt72
type: Position
type: ExactPosition
position: 72
reference: http://purl.uniprot.org/isoforms/A0A482D308-1

-9218568810373570514tt475
type: Position
type: ExactPosition
position: 475
reference: http://purl.uniprot.org/isoforms/A0A482D308-1

-9218568810373570514tt478
type: Position
type: ExactPosition
position: 478
reference: http://purl.uniprot.org/isoforms/A0A482D308-1

-9218568810373570514tt500
type: Position
type: ExactPosition
position: 500
reference: http://purl.uniprot.org/isoforms/A0A482D308-1

-9218568810373570514tt503
type: Position
type: ExactPosition
position: 503
reference: http://purl.uniprot.org/isoforms/A0A482D308-1

SIP46D25C170EA7D39E
type: Mutagenesis Annotation
comment: About 15% cleavage of target dsDNA.
range: -9218568810373570514tt39tt72

-9218568810373570514tt39
type: Position
type: ExactPosition
position: 39
reference: http://purl.uniprot.org/isoforms/A0A482D308-1

SIP76943DAC08C48B07
type: Mutagenesis Annotation
comment: Loss of cleavage of target dsDNA cleavage, binds sgRNA; when associated with R-178.
substitution: RELYAEIFR
range: -9218568810373570514tt118tt126

-9218568810373570514tt118
type: Position
type: ExactPosition
position: 118
reference: http://purl.uniprot.org/isoforms/A0A482D308-1

-9218568810373570514tt126
type: Position
type: ExactPosition
position: 126
reference: http://purl.uniprot.org/isoforms/A0A482D308-1

SIPA381C8A22B753B80
type: Mutagenesis Annotation
comment: Almost complete loss of target dsDNA cleavage.
substitution: R
range: -9218568810373570514tt118tt118

SIP11E9115B8CEAA3C0
type: Mutagenesis Annotation
comment: About 80% cleavage of target dsDNA.
substitution: A
range: -9218568810373570514tt122tt122

-9218568810373570514tt122
type: Position
type: ExactPosition
position: 122
reference: http://purl.uniprot.org/isoforms/A0A482D308-1

SIP4803630EF702F23A
type: Mutagenesis Annotation
comment: About 60% cleavage of target dsDNA.
substitution: R
range: -9218568810373570514tt126tt126

SIPE51D6BAD7755CBD4
type: Mutagenesis Annotation
comment: No cleavage of target dsDNA cleavage.
substitution: A
range: -9218568810373570514tt146tt146

-9218568810373570514tt146
type: Position
type: ExactPosition
position: 146
reference: http://purl.uniprot.org/isoforms/A0A482D308-1

SIP5833B5B2F6F79FAA
type: Mutagenesis Annotation
comment: About 40% cleavage of target dsDNA, loss of cleavage, binds sgRNA; when associated with 118-R--R-126.
substitution: R
range: -9218568810373570514tt178tt178

-9218568810373570514tt178
type: Position
type: ExactPosition
position: 178
reference: http://purl.uniprot.org/isoforms/A0A482D308-1

SIP5E6F4841DBEA1A5D
type: Mutagenesis Annotation
comment: About 30% cleavage of target dsDNA.
substitution: A
range: -9218568810373570514tt197tt197

-9218568810373570514tt197
type: Position
type: ExactPosition
position: 197
reference: http://purl.uniprot.org/isoforms/A0A482D308-1

SIPC83430F6C6A0DD07
type: Mutagenesis Annotation
comment: About 8% cleavage of target dsDNA.
substitution: A
range: -9218568810373570514tt198tt198

-9218568810373570514tt198
type: Position
type: ExactPosition
position: 198
reference: http://purl.uniprot.org/isoforms/A0A482D308-1

SIPA98DB366538951BA
type: Mutagenesis Annotation
comment: Nearly wild-type cleavage of target dsDNA.
substitution: A
range: -9218568810373570514tt286tt286

-9218568810373570514tt286
type: Position
type: ExactPosition
position: 286
reference: http://purl.uniprot.org/isoforms/A0A482D308-1

SIP70930C4B5827C784
type: Mutagenesis Annotation
comment: No cleavage of target ssDNA. No cleavage of supercoiled target dsDNA.
substitution: A
range: -9218568810373570514tt326tt326

SIP1A28A5B354BB419F
type: Mutagenesis Annotation
comment: No cleavage of target dsDNA cleavage.
range: -9218568810373570514tt366tt383

-9218568810373570514tt366
type: Position
type: ExactPosition
position: 366
reference: http://purl.uniprot.org/isoforms/A0A482D308-1

-9218568810373570514tt383
type: Position
type: ExactPosition
position: 383
reference: http://purl.uniprot.org/isoforms/A0A482D308-1

SIP698A046346126F94
type: Mutagenesis Annotation
comment: No cleavage of target ssDNA.
substitution: A
range: -9218568810373570514tt422tt422

SIP791D1140EB885A8A
type: Mutagenesis Annotation
comment: About 60% cleavage of target dsDNA.
substitution: A
range: -9218568810373570514tt487tt487

-9218568810373570514tt487
type: Position
type: ExactPosition
position: 487
reference: http://purl.uniprot.org/isoforms/A0A482D308-1

SIP69B1E558A82FF4BB
type: Mutagenesis Annotation
comment: No cleavage of target ssDNA.
substitution: A
range: -9218568810373570514tt490tt490

SIP2054C9F798C7C535
type: Mutagenesis Annotation
comment: No cleavage of target ssDNA. No cleavage of supercoiled target dsDNA.
substitution: A
range: -9218568810373570514tt510tt510

1
subclass of: CHEBI_29105

2
subclass of: CHEBI_29105

http://purl.uniprot.org/isoforms/A0A482D308-1
type: Simple Sequence
modified: 2019-06-05
version: 1
mass: 61517
value: MAKNTITKTLKLRIVRPYNSAEVEKIVADEKNNREKIALEKNKDKVKEACSKHLKVAAYCTTQVERNACLFCKARKLDDKFYQKLRGQFPDAVFWQEISEIFRQLQKQAAEIYNQSLIELYYEIFIKGKGIANASSVEHYLSDVCYTRAAELFKNAAIASGLRSKIKSNFRLKELKNMKSGLPTTKSDNFPIPLVKQKGGQYTGFEISNHNSDFIIKIPFGRWQVKKEIDKYRPWEKFDFEQVQKSPKPISLLLSTQRRKRNKGWSKDEGTEAEIKKVMNGDYQTSYIEVKRGSKIGEKSAWMLNLSIDVPKIDKGVDPSIIGGIDVGVKSPLVCAINNAFSRYSISDNDLFHFNKKMFARRRILLKKNRHKRAGHGAKNKLKPITILTEKSERFRKKLIERWACEIADFFIKNKVGTVQMENLESMKRKEDSYFNIRLRGFWPYAEMQNKIEFKLKQYGIEIRKVAPNNTSKTCSKCGHLNNYFNFEYRKKNKFPHFKCEKCNFKENADYNAALNISNPKLKSTKEEP

http://purl.uniprot.org/uniprot/A0A482D308#attribution-63EBFA6888CCE9223A3BDC8B15283BB2
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/30337455

http://purl.uniprot.org/uniprot/A0A482D308#attribution-E9DF6F5BDB34333E658E0645D83A1589
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/32246713

http://purl.uniprot.org/uniprot/A0A482D308#attribution-E7DB46EEFBE53ADB5F8226D0D92212EF
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/33333018

http://purl.uniprot.org/uniprot/A0A482D308#attribution-74410C1CA83B0AFC3F906C1E5AC670F2
manual: true
evidence: ECO_0000303
source: http://purl.uniprot.org/citations/30337455

http://purl.uniprot.org/uniprot/A0A482D308#attribution-EED15768E057F2989E614460008AC62E
manual: true
evidence: ECO_0000303
source: http://purl.uniprot.org/citations/31857715

http://purl.uniprot.org/uniprot/A0A482D308#attribution-231C280F16F856186A6A2EC2C9B17AAB
manual: true
evidence: ECO_0000303
source: http://purl.uniprot.org/citations/32246713

http://purl.uniprot.org/uniprot/A0A482D308#attribution-F17018E68EA8D3F3DE00C95AD2074FFC
manual: true
evidence: ECO_0000305
source: http://purl.uniprot.org/citations/30337455

http://purl.uniprot.org/uniprot/A0A482D308#attribution-2F824B1B815B1B497245CC914B90C309
manual: true
evidence: ECO_0000305
source: http://purl.uniprot.org/citations/32246713

http://purl.uniprot.org/uniprot/A0A482D308#attribution-479FE137FA2D6028C8E3CD39C132D197
manual: true
evidence: ECO_0000305
source: http://purl.uniprot.org/citations/33333018

http://purl.uniprot.org/uniprot/A0A482D308#attribution-CE9BB7E32C2B7BB9C11EB6D43C4FCAFF
manual: true
evidence: ECO_0000312
source: QBM01166.1

http://purl.uniprot.org/uniprot/A0A482D308#attribution-227C1FE8210ECC2C900B545A01E5D04A
manual: true
evidence: ECO_0000312
source: 7C7L

http://purl.uniprot.org/uniprot/A0A482D308#attribution-1AD853C526D2D9F3AD948E9CB94A3834
manual: true
evidence: ECO_0007744
source: 7C7L

C0HM55
type: Protein
reviewed: true
created: 2022-12-14
modified: 2023-06-28
version: 4
mnemonic: FSX1_UNCAX
citation: http://purl.uniprot.org/citations/35794124
recommended name: SIP70F504FE42258A4C
organism: http://purl.uniprot.org/taxonomy/115547
annotation: SIPB4849A85912CE65A
annotation: SIPBEE4B3DB0574856F
annotation: SIPA5EE2F171CEBA936
annotation: SIPA377B98080221D04
annotation: SIP293424D3BC6BB655
annotation: SIP793C40EF1D3DAEB2
annotation: SIPA1E96AF335001414
annotation: PRO_0000456978
annotation: SIP2F8AB13C17CF593D
annotation: SIPE5C8D9D8F23BAAEA
annotation: SIPFEA9C5D2E035CF2A
annotation: SIPF69763E22787327C
annotation: SIPA0C515977EB2FC02
annotation: SIPF71148A9471DD04E
annotation: SIPE8962D8CE555AF25
annotation: SIPF1F800153688CFEB
annotation: SIPAA66B1FE3A58A2C2
annotation: SIP46A7518DF0C72B29
annotation: SIPDD820D8D8673188C
annotation: SIPFE09C9813C045A99
annotation: SIP5E5031BD34788675
annotation: SIP402AF716959E1725
annotation: SIP59D4DB90CD6510E1
annotation: SIP741460D0CDCCEAFB
annotation: SIP91A563844E25FE0A
annotation: SIPB8497A63F8E043C0
annotation: SIPFBBACCE345530485
annotation: SIPB8C118A0DEFCA9B6
annotation: SIPB417AD587A6D1B45
annotation: SIP7884CAE104903A4C
annotation: SIP930528FB8B5039CA
annotation: SIP2C076AFCC9348A9E
annotation: SIP8E13F9523EA2A700
annotation: SIPBE01842AA592112F
annotation: SIP74D72AABD0FB2B30
annotation: SIP7B2D68475B16E368
annotation: SIPCD555015F86AC981
annotation: SIP2E839468D1CC1956
annotation: SIP645DA2ED51E95138
annotation: SIPFE5CEB528281938D
annotation: SIPC902E18A5242E74A
annotation: SIP319992EC82672C09
annotation: SIP9CB7CD5C63D4A1DC
annotation: SIPBEEC31BF6A1E1641
annotation: SIPC16D2E1B165750F3
annotation: SIP9D55DE22B2DAC835
annotation: SIPF5517868110075D0
annotation: SIP4F4C344E86B137ED
annotation: SIP85701BE488F9EF7C
annotation: SIPD872CE8A967E0E7E
annotation: SIPDE146790EFA09CD4
annotation: SIP6512841E750CB791
annotation: SIP6BB723C2E129B363
annotation: SIPC6B0A9A727213F9E
annotation: SIPF749DA763BB81CA8
annotation: SIPFA9A095A99C20B37
annotation: SIPD29A57608566E76D
annotation: SIP2D5B412FA472BEA6
annotation: SIP83CBAB85EDF36A1C
annotation: SIPAEB8A2DC8E1F8281
annotation: SIPD64FEFF09EB5F7D9
annotation: SIP5C270F489CD42F7F
annotation: SIP48387777B98EF478
annotation: SIPDAB55D7642624C11
annotation: SIP32133F66AF537BDD
annotation: SIP4070134695CD46DB
annotation: SIP6FACC7E641DC0775
annotation: SIPE0F5920189EEE9A1
annotation: SIPAA05DBB83B618174
annotation: SIPE58EC0889C14390C
annotation: SIP08621CFD804AE5B4

C0HM55-citation-35794124
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
scope: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 25-535
scope: FUNCTION
scope: SUBUNIT
scope: SUBCELLULAR LOCATION
scope: DOMAIN
scope: DISULFIDE BOND
scope: TOPOLOGY
scope: MUTAGENESIS OF 142-TYR--TYR-149

7P4L
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.30
chain sequence mapping: PDB 7P4L tt25tt535

PDB 7P4L tt25tt535
chain: A/B/C=25-535

1.N.9.1.1
type: Resource
database: TCDB
comment: the fusexin1 (fsx1) family

http://purl.uniprot.org/gene3d/2.60.40.10
type: Resource
database: Gene3D
comment: Immunoglobulins
signature sequence match: Gene3D 2.60.40.10 match 1

MF_00869
type: Resource
database: HAMAP
comment: Fusexin_1
signature sequence match: HAMAP MF 00869 match 1

IPR013783
type: Resource
database: InterPro
comment: Ig-like_fold

SIP70F504FE42258A4C
type: Structured Name
full name: Fusexin 1
short name: Fsx1

fsx1
type: Gene

SIPB4849A85912CE65A
type: Function Annotation
comment: Exhibits fusogenic activity (PubMed:35794124). Mediates cell-cell fusion in mammalian cells when present in both cells (bilateral fusion) (PubMed:35794124).

SIPBEE4B3DB0574856F
type: Subunit Annotation
comment: Monomer in solution, crystallizes as a trimer in high salt (2.5 M NaCl, 0.2 M CaCl(2)). The trimer is stabilized by interdomain contacts and numerous Ca(2+) and Na(+) ions.

SIPA377B98080221D04
type: Domain Annotation
comment: The extracellular N-terminus has 4 domains; the first 3 are structurally similar to fusogens from plants, C.elegans and viruses, while the fourth domain is unique to archaea. Domains I and II are discontinuous, domain IV cannot be replaced. The protein can be anchored to the membrane by either a different transmembrane helix or by a GPI-anchored construct and still directs membrane fusion. The fusion loop in domain II is stabilized by a Ca(2+) ion so that it protrudes from the molecule.

SIP293424D3BC6BB655
type: Annotation
comment: This entry represents a metagenomic sequence isolated from an uncultured archaeon from a hypersaline environment.

SIP793C40EF1D3DAEB2
type: Similarity Annotation
comment: Belongs to the HAP2/GCS1 family. Fusexin 1 subfamily.

18911910530725166tt1
type: Position
type: ExactPosition
position: 1
reference: C0HM55-1

18911910530725166tt19
type: Position
type: ExactPosition
position: 19
reference: C0HM55-1

PRO_0000456978
type: Chain Annotation
comment: Fusexin 1
mass: 65843
range: 18911910530725166tt20tt631

18911910530725166tt20
type: Position
type: ExactPosition
position: 20
reference: C0HM55-1

18911910530725166tt631
type: Position
type: ExactPosition
position: 631
reference: C0HM55-1

SIP2F8AB13C17CF593D
type: Topological Domain Annotation
comment: Extracellular
range: 18911910530725166tt20tt537

18911910530725166tt537
type: Position
type: ExactPosition
position: 537
reference: C0HM55-1

SIPE5C8D9D8F23BAAEA
type: Transmembrane Annotation
comment: Helical
range: 18911910530725166tt538tt558

18911910530725166tt538
type: Position
type: ExactPosition
position: 538
reference: C0HM55-1

18911910530725166tt558
type: Position
type: ExactPosition
position: 558
reference: C0HM55-1

SIPFEA9C5D2E035CF2A
type: Topological Domain Annotation
comment: Cytoplasmic
range: 18911910530725166tt559tt590

18911910530725166tt559
type: Position
type: ExactPosition
position: 559
reference: C0HM55-1

18911910530725166tt590
type: Position
type: ExactPosition
position: 590
reference: C0HM55-1

SIPF69763E22787327C
type: Transmembrane Annotation
comment: Helical
range: 18911910530725166tt591tt611

18911910530725166tt591
type: Position
type: ExactPosition
position: 591
reference: C0HM55-1

18911910530725166tt611
type: Position
type: ExactPosition
position: 611
reference: C0HM55-1

SIPA0C515977EB2FC02
type: Topological Domain Annotation
comment: Extracellular
range: 18911910530725166tt612tt612

18911910530725166tt612
type: Position
type: ExactPosition
position: 612
reference: C0HM55-1

SIPF71148A9471DD04E
type: Transmembrane Annotation
comment: Helical
range: 18911910530725166tt613tt630

18911910530725166tt613
type: Position
type: ExactPosition
position: 613
reference: C0HM55-1

18911910530725166tt630
type: Position
type: ExactPosition
position: 630
reference: C0HM55-1

SIPE8962D8CE555AF25
type: Topological Domain Annotation
comment: Cytoplasmic
range: 18911910530725166tt631tt631

SIPF1F800153688CFEB
type: Region Annotation
comment: Domain I N-terminus
range: 18911910530725166tt20tt90

18911910530725166tt90
type: Position
type: ExactPosition
position: 90
reference: C0HM55-1

SIPAA66B1FE3A58A2C2
type: Region Annotation
comment: Domain II N-terminus
range: 18911910530725166tt91tt170

18911910530725166tt91
type: Position
type: ExactPosition
position: 91
reference: C0HM55-1

18911910530725166tt170
type: Position
type: ExactPosition
position: 170
reference: C0HM55-1

SIP46A7518DF0C72B29
type: Region Annotation
comment: Fusion loop, required for fusogenic activity, not required for membrane surface localization
range: 18911910530725166tt143tt148

18911910530725166tt143
type: Position
type: ExactPosition
position: 143
reference: C0HM55-1

18911910530725166tt148
type: Position
type: ExactPosition
position: 148
reference: C0HM55-1

SIPDD820D8D8673188C
type: Region Annotation
comment: Domain I central section
range: 18911910530725166tt171tt224

18911910530725166tt171
type: Position
type: ExactPosition
position: 171
reference: C0HM55-1

18911910530725166tt224
type: Position
type: ExactPosition
position: 224
reference: C0HM55-1

SIPFE09C9813C045A99
type: Region Annotation
comment: Domain II C-terminus
range: 18911910530725166tt225tt316

18911910530725166tt225
type: Position
type: ExactPosition
position: 225
reference: C0HM55-1

18911910530725166tt316
type: Position
type: ExactPosition
position: 316
reference: C0HM55-1

SIP5E5031BD34788675
type: Region Annotation
comment: Domain I C-terminus
range: 18911910530725166tt317tt348

18911910530725166tt317
type: Position
type: ExactPosition
position: 317
reference: C0HM55-1

18911910530725166tt348
type: Position
type: ExactPosition
position: 348
reference: C0HM55-1

SIP402AF716959E1725
type: Region Annotation
comment: Domain III
range: 18911910530725166tt349tt455

18911910530725166tt349
type: Position
type: ExactPosition
position: 349
reference: C0HM55-1

18911910530725166tt455
type: Position
type: ExactPosition
position: 455
reference: C0HM55-1

SIP59D4DB90CD6510E1
type: Region Annotation
comment: Disordered
range: 18911910530725166tt443tt467

18911910530725166tt443
type: Position
type: ExactPosition
position: 443
reference: C0HM55-1

18911910530725166tt467
type: Position
type: ExactPosition
position: 467
reference: C0HM55-1

SIP741460D0CDCCEAFB
type: Region Annotation
comment: Domain IV, required for fusogenic activity
range: 18911910530725166tt456tt509

18911910530725166tt456
type: Position
type: ExactPosition
position: 456
reference: C0HM55-1

18911910530725166tt509
type: Position
type: ExactPosition
position: 509
reference: C0HM55-1

SIP91A563844E25FE0A
type: Region Annotation
comment: Stem
range: 18911910530725166tt510tt537

18911910530725166tt510
type: Position
type: ExactPosition
position: 510
reference: C0HM55-1

SIPB8497A63F8E043C0
type: Compositional Bias Annotation
comment: Polar residues
range: 18911910530725166tt443tt458

18911910530725166tt458
type: Position
type: ExactPosition
position: 458
reference: C0HM55-1

18911910530725166tt112
type: Position
type: ExactPosition
position: 112
reference: C0HM55-1

18911910530725166tt146
type: Position
type: ExactPosition
position: 146
reference: C0HM55-1

18911910530725166tt149
type: Position
type: ExactPosition
position: 149
reference: C0HM55-1

18911910530725166tt150
type: Position
type: ExactPosition
position: 150
reference: C0HM55-1

18911910530725166tt125
type: Position
type: ExactPosition
position: 125
reference: C0HM55-1

18911910530725166tt155
type: Position
type: ExactPosition
position: 155
reference: C0HM55-1

18911910530725166tt389
type: Position
type: ExactPosition
position: 389
reference: C0HM55-1

18911910530725166tt432
type: Position
type: ExactPosition
position: 432
reference: C0HM55-1

18911910530725166tt457
type: Position
type: ExactPosition
position: 457
reference: C0HM55-1

18911910530725166tt477
type: Position
type: ExactPosition
position: 477
reference: C0HM55-1

18911910530725166tt490
type: Position
type: ExactPosition
position: 490
reference: C0HM55-1

18911910530725166tt506
type: Position
type: ExactPosition
position: 506
reference: C0HM55-1

SIP74D72AABD0FB2B30
type: Mutagenesis Annotation
comment: Loss of fusogenic activity, still found on cell surface (in mammalian cells).
substitution: AGGGGA
range: 18911910530725166tt142tt149

18911910530725166tt142
type: Position
type: ExactPosition
position: 142
reference: C0HM55-1

18911910530725166tt27
type: Position
type: ExactPosition
position: 27
reference: C0HM55-1

18911910530725166tt30
type: Position
type: ExactPosition
position: 30
reference: C0HM55-1

18911910530725166tt36
type: Position
type: ExactPosition
position: 36
reference: C0HM55-1

18911910530725166tt38
type: Position
type: ExactPosition
position: 38
reference: C0HM55-1

18911910530725166tt42
type: Position
type: ExactPosition
position: 42
reference: C0HM55-1

18911910530725166tt47
type: Position
type: ExactPosition
position: 47
reference: C0HM55-1

18911910530725166tt54
type: Position
type: ExactPosition
position: 54
reference: C0HM55-1

18911910530725166tt58
type: Position
type: ExactPosition
position: 58
reference: C0HM55-1

18911910530725166tt76
type: Position
type: ExactPosition
position: 76
reference: C0HM55-1

18911910530725166tt88
type: Position
type: ExactPosition
position: 88
reference: C0HM55-1

18911910530725166tt100
type: Position
type: ExactPosition
position: 100
reference: C0HM55-1

18911910530725166tt113
type: Position
type: ExactPosition
position: 113
reference: C0HM55-1

18911910530725166tt114
type: Position
type: ExactPosition
position: 114
reference: C0HM55-1

18911910530725166tt124
type: Position
type: ExactPosition
position: 124
reference: C0HM55-1

18911910530725166tt131
type: Position
type: ExactPosition
position: 131
reference: C0HM55-1

18911910530725166tt133
type: Position
type: ExactPosition
position: 133
reference: C0HM55-1

18911910530725166tt136
type: Position
type: ExactPosition
position: 136
reference: C0HM55-1

18911910530725166tt144
type: Position
type: ExactPosition
position: 144
reference: C0HM55-1

18911910530725166tt168
type: Position
type: ExactPosition
position: 168
reference: C0HM55-1

18911910530725166tt172
type: Position
type: ExactPosition
position: 172
reference: C0HM55-1

18911910530725166tt198
type: Position
type: ExactPosition
position: 198
reference: C0HM55-1

18911910530725166tt201
type: Position
type: ExactPosition
position: 201
reference: C0HM55-1

18911910530725166tt206
type: Position
type: ExactPosition
position: 206
reference: C0HM55-1

18911910530725166tt209
type: Position
type: ExactPosition
position: 209
reference: C0HM55-1

18911910530725166tt218
type: Position
type: ExactPosition
position: 218
reference: C0HM55-1

18911910530725166tt226
type: Position
type: ExactPosition
position: 226
reference: C0HM55-1

18911910530725166tt228
type: Position
type: ExactPosition
position: 228
reference: C0HM55-1

18911910530725166tt229
type: Position
type: ExactPosition
position: 229
reference: C0HM55-1

18911910530725166tt233
type: Position
type: ExactPosition
position: 233
reference: C0HM55-1

18911910530725166tt238
type: Position
type: ExactPosition
position: 238
reference: C0HM55-1

18911910530725166tt240
type: Position
type: ExactPosition
position: 240
reference: C0HM55-1

18911910530725166tt241
type: Position
type: ExactPosition
position: 241
reference: C0HM55-1

18911910530725166tt245
type: Position
type: ExactPosition
position: 245
reference: C0HM55-1

18911910530725166tt246
type: Position
type: ExactPosition
position: 246
reference: C0HM55-1

18911910530725166tt258
type: Position
type: ExactPosition
position: 258
reference: C0HM55-1

18911910530725166tt260
type: Position
type: ExactPosition
position: 260
reference: C0HM55-1

18911910530725166tt269
type: Position
type: ExactPosition
position: 269
reference: C0HM55-1

18911910530725166tt275
type: Position
type: ExactPosition
position: 275
reference: C0HM55-1

18911910530725166tt281
type: Position
type: ExactPosition
position: 281
reference: C0HM55-1

18911910530725166tt285
type: Position
type: ExactPosition
position: 285
reference: C0HM55-1

18911910530725166tt290
type: Position
type: ExactPosition
position: 290
reference: C0HM55-1

18911910530725166tt296
type: Position
type: ExactPosition
position: 296
reference: C0HM55-1

18911910530725166tt298
type: Position
type: ExactPosition
position: 298
reference: C0HM55-1

18911910530725166tt304
type: Position
type: ExactPosition
position: 304
reference: C0HM55-1

18911910530725166tt308
type: Position
type: ExactPosition
position: 308
reference: C0HM55-1

18911910530725166tt313
type: Position
type: ExactPosition
position: 313
reference: C0HM55-1

18911910530725166tt315
type: Position
type: ExactPosition
position: 315
reference: C0HM55-1

18911910530725166tt319
type: Position
type: ExactPosition
position: 319
reference: C0HM55-1

18911910530725166tt325
type: Position
type: ExactPosition
position: 325
reference: C0HM55-1

18911910530725166tt333
type: Position
type: ExactPosition
position: 333
reference: C0HM55-1

18911910530725166tt340
type: Position
type: ExactPosition
position: 340
reference: C0HM55-1

18911910530725166tt344
type: Position
type: ExactPosition
position: 344
reference: C0HM55-1

18911910530725166tt350
type: Position
type: ExactPosition
position: 350
reference: C0HM55-1

18911910530725166tt357
type: Position
type: ExactPosition
position: 357
reference: C0HM55-1

18911910530725166tt366
type: Position
type: ExactPosition
position: 366
reference: C0HM55-1

18911910530725166tt374
type: Position
type: ExactPosition
position: 374
reference: C0HM55-1

18911910530725166tt376
type: Position
type: ExactPosition
position: 376
reference: C0HM55-1

18911910530725166tt378
type: Position
type: ExactPosition
position: 378
reference: C0HM55-1

18911910530725166tt380
type: Position
type: ExactPosition
position: 380
reference: C0HM55-1

18911910530725166tt393
type: Position
type: ExactPosition
position: 393
reference: C0HM55-1

18911910530725166tt395
type: Position
type: ExactPosition
position: 395
reference: C0HM55-1

18911910530725166tt400
type: Position
type: ExactPosition
position: 400
reference: C0HM55-1

18911910530725166tt404
type: Position
type: ExactPosition
position: 404
reference: C0HM55-1

18911910530725166tt409
type: Position
type: ExactPosition
position: 409
reference: C0HM55-1

18911910530725166tt418
type: Position
type: ExactPosition
position: 418
reference: C0HM55-1

18911910530725166tt426
type: Position
type: ExactPosition
position: 426
reference: C0HM55-1

18911910530725166tt437
type: Position
type: ExactPosition
position: 437
reference: C0HM55-1

18911910530725166tt442
type: Position
type: ExactPosition
position: 442
reference: C0HM55-1

18911910530725166tt452
type: Position
type: ExactPosition
position: 452
reference: C0HM55-1

18911910530725166tt462
type: Position
type: ExactPosition
position: 462
reference: C0HM55-1

18911910530725166tt466
type: Position
type: ExactPosition
position: 466
reference: C0HM55-1

18911910530725166tt472
type: Position
type: ExactPosition
position: 472
reference: C0HM55-1

18911910530725166tt484
type: Position
type: ExactPosition
position: 484
reference: C0HM55-1

18911910530725166tt489
type: Position
type: ExactPosition
position: 489
reference: C0HM55-1

18911910530725166tt495
type: Position
type: ExactPosition
position: 495
reference: C0HM55-1

18911910530725166tt498
type: Position
type: ExactPosition
position: 498
reference: C0HM55-1

18911910530725166tt504
type: Position
type: ExactPosition
position: 504
reference: C0HM55-1

18911910530725166tt507
type: Position
type: ExactPosition
position: 507
reference: C0HM55-1

C0HM55-1
type: Simple Sequence
modified: 2022-12-14
version: 1
precursor: true
mass: 67874
value: MRRAALILAFVLFIGLSSATVTSADSITYNSGTSEFFDGDVFAIEVTADQSTDEIDIYLGASELSEKTDGEVNQDLSIEFTHQDSKLKYSTSTSDELRDIVTLTTYYEDGFDTEQDAIDAIKSDCYDLNQNGNGSGRYSRYYSVTSPVYDYEIYCFQKNEKLATPAYIDNPDEIFTAKAELQAGDKTIQSATLSNGDAGDGTVTDLGDSKISWNGNLDLGASEPENSRVIALYSNDFENGWRIGNKQSYEDYKTFIGGGDAYDLLIDWQDGTYTASEVEDELVNTDANQAVEEASSSTTDLVNAKVKDSSLDTGSFVYDTPELLSYPSFTVYVDAGENGYIEVTKPTGDPDIISTSSTEIKEGDEGTVTATVENVGDGEGEFSGRLSSCGEGFSIVDDQNTKNVGAGESVTYSFDVAFSSVSSESKEISGSCTFEVNGVESSDSTSVSVTGIQQSECNPGDQRREKNENDRWEIYTCQDNGLTYEYDVTCAEDEKAVAQGDNQFSCEKQDDDSGGGDNTGSDSGLFSNLFGGSGSGDLLTQVHTALSILAGLVAGFFGYRGARWIHGETDIKGGFKLESRNVSRVKRGSPVAGIVGAVLGFVVGYGVASVFHPVVQIIVVLGIAVGLYYFR

http://purl.uniprot.org/uniprot/C0HM55#attribution-8C494B32D67E3D851C274812CC4F52C4
creator: UniProtKB
manual: true
evidence: ECO_0000314
source: http://purl.uniprot.org/citations/35794124

http://purl.uniprot.org/uniprot/C0HM55#attribution-F51652B141212960DDA1104870B52EFA
manual: true
evidence: ECO_0000255
source: MF_00869

http://purl.uniprot.org/uniprot/C0HM55#attribution-07E1EE9C59A8B868B49A3BB99410CD0E
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/35794124

http://purl.uniprot.org/uniprot/C0HM55#attribution-73A18A42957D9B14185A8F2EC821B801
manual: true
evidence: ECO_0000303
source: http://purl.uniprot.org/citations/35794124

http://purl.uniprot.org/uniprot/C0HM55#attribution-4266AF1FF19A141062E7BB05EBA5D81D
manual: true
evidence: ECO_0000305
source: http://purl.uniprot.org/citations/35794124

http://purl.uniprot.org/uniprot/C0HM55#attribution-E2A9062AB377B70C00309B4518542C2F
manual: true
evidence: ECO_0000312
source: 7P4L

http://purl.uniprot.org/uniprot/C0HM55#attribution-156EF946D28C2767063ACCAC0F1AF756
manual: true
evidence: ECO_0007744
source: 7P4L

Q64BR7
type: Protein
reviewed: true
created: 2016-07-06
modified: 2023-09-13
version: 84
mnemonic: ILVC_UNCAG
citation: http://purl.uniprot.org/citations/15353801
citation: http://purl.uniprot.org/citations/25849365
recommended name: SIPF0533357D214E145
alternative name: SIP47F07242834F7C3D
alternative name: SIP959BBCAD0EF88C41
alternative name: SIP7B7F77015CDC018C
alternative name: SIPA2A4BE1FDEA70E4B
enzyme: http://purl.uniprot.org/enzyme/1.1.1.382
organism: http://purl.uniprot.org/taxonomy/285389
annotation: SIP59C4E0647313D6F0
annotation: SIP64D47E88FC2308F3
annotation: SIP079D646D24F5BFE1
annotation: SIP2DB2AF2B894220A3
annotation: SIP8764A1A8B6BC0D0B
annotation: SIP68643DDB867D6571
annotation: SIPA592E01151D9B609
annotation: PRO_0000436836
annotation: SIPF7D43AB89E84113A
annotation: SIP1ED1DAD2ECB51E76
annotation: SIPDAD465B3478B2328
annotation: SIPAD43D84CA5668CF4
annotation: SIP7A70CED87D61CAF6
annotation: SIPBD57D051DA90E0C3
annotation: SIPEE688EC4DEFCCA26
annotation: SIPAADBF0941BB0452E
annotation: SIPC97A2F4AE925E951
annotation: SIP82AF17963B2A56D3
annotation: SIP30D6C416DD06A489
annotation: SIPADF5549DA96B67A8
annotation: SIP672B6DB238FF299D
annotation: SIP74DABFBF5E3A2D27
annotation: SIPFA1E52568596AD3C
annotation: SIP85960C408C556B1E
annotation: SIPE3B1992F689EE6C0
annotation: SIP5D28BC00AB7A8653
annotation: SIP006A730B2BC6B337
annotation: SIP40316E6BA3F149AE
annotation: SIP1633287C62DFBBF7
annotation: SIP933D09C74E90C261
annotation: SIP38FCC88AC535A74A
annotation: SIP97F97EF1CD7EC59F
annotation: SIP13997BF791D0217A
annotation: SIP7E3999C63CE638B8
annotation: SIPBA8E3F90B31CD33C
annotation: SIPFC97FBC915A15FE7
annotation: SIP89A8EBD30DD0A9D4
annotation: SIPCA9F3A8A4766E7D0
annotation: SIPC5295C19BD778395
annotation: SIPB71622F69D764883
annotation: SIP723E1F0F1C6AC17D
annotation: SIP2B4B9B9FCF65EE7C
annotation: SIP229EACC09CBDDBF3
annotation: SIP086D9A6A825BD04C
annotation: SIP174056F12BEB3E90
annotation: SIP3CFD3073C6602AD8
annotation: SIPDF2AF25C0BA8D152
annotation: SIP1C3C1D86660D2FF5
annotation: SIPDB1C14C0F37473A7
annotation: SIPABB0729AE5A0D76D
annotation: SIP64408738B9F84040
annotation: SIPCCD552699A402E5E
annotation: SIPE010AC70885BD74F
annotation: SIPF517DFA107AA5D9B
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/2
classified with: http://purl.uniprot.org/keywords/100
classified with: http://purl.uniprot.org/keywords/460
classified with: http://purl.uniprot.org/keywords/479
classified with: http://purl.uniprot.org/keywords/520
classified with: http://purl.uniprot.org/keywords/560
classified with: GO_0004455
classified with: GO_0000287
classified with: GO_0050661
classified with: GO_0009097

Q64BR7-citation-15353801
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

Q64BR7-citation-25849365
type: Citation Statement
scope: X-RAY CRYSTALLOGRAPHY (1.53 ANGSTROMS) IN COMPLEX WITH NAD AND MAGNESIUM IONS
scope: FUNCTION
scope: COFACTOR
scope: SUBUNIT

4XDY
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 1.53
chain sequence mapping: PDB 4XDY tt1tt332

PDB 4XDY tt1tt332
chain: A/B=1-332

http://purl.uniprot.org/brenda/1.1.1.382
type: Resource
database: BRENDA
comment: 12627

http://purl.uniprot.org/brenda/1.1.1.86
type: Resource
database: BRENDA
comment: 12627

http://purl.uniprot.org/gene3d/3.40.50.720
type: Resource
database: Gene3D
comment: NAD(P)-binding Rossmann-like Domain
signature sequence match: Gene3D 3.40.50.720 match 1

MF_00435
type: Resource
database: HAMAP
comment: IlvC
signature sequence match: HAMAP MF 00435 match 1

IPR008927
type: Resource
database: InterPro
comment: 6-PGluconate_DH-like_C_sf

IPR013023
type: Resource
database: InterPro
comment: KARI

IPR000506
type: Resource
database: InterPro
comment: KARI_C

IPR013116
type: Resource
database: InterPro
comment: KARI_N

IPR014359
type: Resource
database: InterPro
comment: KARI_prok

IPR036291
type: Resource
database: InterPro
comment: NAD(P)-bd_dom_sf

TIGR00465
type: Resource
database: NCBIfam
comment: ilvC
signature sequence match: NCBIfam TIGR00465 match 1

PTHR21371
type: Resource
database: PANTHER
comment: KETOL-ACID REDUCTOISOMERASE, MITOCHONDRIAL
signature sequence match: PANTHER PTHR21371 match 1

PTHR21371:SF1
type: Resource
database: PANTHER
comment: KETOL-ACID REDUCTOISOMERASE, MITOCHONDRIAL
signature sequence match: PANTHER PTHR21371:SF1 match 1

PF01450
type: Resource
database: Pfam
comment: IlvC
signature sequence match: Pfam PF01450 match 1

PF07991
type: Resource
database: Pfam
comment: IlvN
signature sequence match: Pfam PF07991 match 1

PIRSF000116
type: Resource
database: PIRSF
comment: IlvC_gammaproteo
signature sequence match: PIRSF PIRSF000116 match 1

SSF48179
type: Resource
database: SUPFAM
comment: 6-phosphogluconate dehydrogenase C-terminal domain-like
signature sequence match: SUPFAM SSF48179 match 1

SSF51735
type: Resource
database: SUPFAM
comment: NAD(P)-binding Rossmann-fold domains
signature sequence match: SUPFAM SSF51735 match 1

PS51851
type: Resource
database: PROSITE
comment: KARI_C
signature sequence match: PROSITE PS51851 match 1

PS51850
type: Resource
database: PROSITE
comment: KARI_N
signature sequence match: PROSITE PS51850 match 1

SIPF0533357D214E145
type: Structured Name
full name: Ketol-acid reductoisomerase (NAD(+))
short name: KARI
ec name: 1.1.1.382

SIP47F07242834F7C3D
type: Structured Name
full name: Acetohydroxy-acid isomeroreductase
short name: AHIR

SIP959BBCAD0EF88C41
type: Structured Name
full name: Alpha-keto-beta-hydroxylacyl reductoisomerase

SIP7B7F77015CDC018C
type: Structured Name
full name: Ketol-acid reductoisomerase type 1

SIPA2A4BE1FDEA70E4B
type: Structured Name
full name: Ketol-acid reductoisomerase type I

ilvC
type: Gene
orf name: GZ26G2_30

SIP59C4E0647313D6F0
type: Function Annotation
comment: Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADH to yield (R)-2,3-dihydroxy-isovalerate.

SIP079D646D24F5BFE1
type: Cofactor Annotation
comment: Binds 2 magnesium ions per subunit.
cofactor: CHEBI_18420

SIP68643DDB867D6571
type: Subunit Annotation
comment: Homodimer.

SIPA592E01151D9B609
type: Similarity Annotation
comment: Belongs to the ketol-acid reductoisomerase family.

PRO_0000436836
type: Chain Annotation
comment: Ketol-acid reductoisomerase (NAD(+))
mass: 36857
range: 22859071192446766tt1tt332

22859071192446766tt1
type: Position
type: ExactPosition
position: 1
reference: Q64BR7-1

22859071192446766tt332
type: Position
type: ExactPosition
position: 332
reference: Q64BR7-1

SIPF7D43AB89E84113A
type: Domain Extent Annotation
comment: KARI N-terminal Rossmann
range: 22859071192446766tt1tt186

22859071192446766tt186
type: Position
type: ExactPosition
position: 186
reference: Q64BR7-1

SIP1ED1DAD2ECB51E76
type: Domain Extent Annotation
comment: KARI C-terminal knotted
range: 22859071192446766tt187tt332

22859071192446766tt187
type: Position
type: ExactPosition
position: 187
reference: Q64BR7-1

22859071192446766tt112
type: Position
type: ExactPosition
position: 112
reference: Q64BR7-1

22859071192446766tt24
type: Position
type: ExactPosition
position: 24
reference: Q64BR7-1

22859071192446766tt27
type: Position
type: ExactPosition
position: 27
reference: Q64BR7-1

22859071192446766tt46
type: Position
type: ExactPosition
position: 46
reference: Q64BR7-1

22859071192446766tt55
type: Position
type: ExactPosition
position: 55
reference: Q64BR7-1

22859071192446766tt57
type: Position
type: ExactPosition
position: 57
reference: Q64BR7-1

22859071192446766tt87
type: Position
type: ExactPosition
position: 87
reference: Q64BR7-1

22859071192446766tt90
type: Position
type: ExactPosition
position: 90
reference: Q64BR7-1

22859071192446766tt138
type: Position
type: ExactPosition
position: 138
reference: Q64BR7-1

22859071192446766tt195
type: Position
type: ExactPosition
position: 195
reference: Q64BR7-1

22859071192446766tt199
type: Position
type: ExactPosition
position: 199
reference: Q64BR7-1

22859071192446766tt231
type: Position
type: ExactPosition
position: 231
reference: Q64BR7-1

22859071192446766tt235
type: Position
type: ExactPosition
position: 235
reference: Q64BR7-1

22859071192446766tt256
type: Position
type: ExactPosition
position: 256
reference: Q64BR7-1

22859071192446766tt6
type: Position
type: ExactPosition
position: 6
reference: Q64BR7-1

22859071192446766tt8
type: Position
type: ExactPosition
position: 8
reference: Q64BR7-1

22859071192446766tt12
type: Position
type: ExactPosition
position: 12
reference: Q64BR7-1

22859071192446766tt16
type: Position
type: ExactPosition
position: 16
reference: Q64BR7-1

22859071192446766tt18
type: Position
type: ExactPosition
position: 18
reference: Q64BR7-1

22859071192446766tt22
type: Position
type: ExactPosition
position: 22
reference: Q64BR7-1

22859071192446766tt26
type: Position
type: ExactPosition
position: 26
reference: Q64BR7-1

22859071192446766tt37
type: Position
type: ExactPosition
position: 37
reference: Q64BR7-1

22859071192446766tt41
type: Position
type: ExactPosition
position: 41
reference: Q64BR7-1

22859071192446766tt48
type: Position
type: ExactPosition
position: 48
reference: Q64BR7-1

22859071192446766tt50
type: Position
type: ExactPosition
position: 50
reference: Q64BR7-1

22859071192446766tt56
type: Position
type: ExactPosition
position: 56
reference: Q64BR7-1

22859071192446766tt63
type: Position
type: ExactPosition
position: 63
reference: Q64BR7-1

22859071192446766tt67
type: Position
type: ExactPosition
position: 67
reference: Q64BR7-1

22859071192446766tt70
type: Position
type: ExactPosition
position: 70
reference: Q64BR7-1

22859071192446766tt71
type: Position
type: ExactPosition
position: 71
reference: Q64BR7-1

22859071192446766tt77
type: Position
type: ExactPosition
position: 77
reference: Q64BR7-1

22859071192446766tt79
type: Position
type: ExactPosition
position: 79
reference: Q64BR7-1

22859071192446766tt83
type: Position
type: ExactPosition
position: 83
reference: Q64BR7-1

22859071192446766tt89
type: Position
type: ExactPosition
position: 89
reference: Q64BR7-1

22859071192446766tt97
type: Position
type: ExactPosition
position: 97
reference: Q64BR7-1

22859071192446766tt99
type: Position
type: ExactPosition
position: 99
reference: Q64BR7-1

22859071192446766tt101
type: Position
type: ExactPosition
position: 101
reference: Q64BR7-1

22859071192446766tt107
type: Position
type: ExactPosition
position: 107
reference: Q64BR7-1

22859071192446766tt113
type: Position
type: ExactPosition
position: 113
reference: Q64BR7-1

22859071192446766tt114
type: Position
type: ExactPosition
position: 114
reference: Q64BR7-1

22859071192446766tt117
type: Position
type: ExactPosition
position: 117
reference: Q64BR7-1

22859071192446766tt127
type: Position
type: ExactPosition
position: 127
reference: Q64BR7-1

22859071192446766tt136
type: Position
type: ExactPosition
position: 136
reference: Q64BR7-1

22859071192446766tt146
type: Position
type: ExactPosition
position: 146
reference: Q64BR7-1

22859071192446766tt153
type: Position
type: ExactPosition
position: 153
reference: Q64BR7-1

22859071192446766tt159
type: Position
type: ExactPosition
position: 159
reference: Q64BR7-1

22859071192446766tt165
type: Position
type: ExactPosition
position: 165
reference: Q64BR7-1

22859071192446766tt175
type: Position
type: ExactPosition
position: 175
reference: Q64BR7-1

22859071192446766tt178
type: Position
type: ExactPosition
position: 178
reference: Q64BR7-1

22859071192446766tt180
type: Position
type: ExactPosition
position: 180
reference: Q64BR7-1

22859071192446766tt183
type: Position
type: ExactPosition
position: 183
reference: Q64BR7-1

22859071192446766tt185
type: Position
type: ExactPosition
position: 185
reference: Q64BR7-1

22859071192446766tt188
type: Position
type: ExactPosition
position: 188
reference: Q64BR7-1

22859071192446766tt201
type: Position
type: ExactPosition
position: 201
reference: Q64BR7-1

22859071192446766tt202
type: Position
type: ExactPosition
position: 202
reference: Q64BR7-1

22859071192446766tt204
type: Position
type: ExactPosition
position: 204
reference: Q64BR7-1

22859071192446766tt205
type: Position
type: ExactPosition
position: 205
reference: Q64BR7-1

22859071192446766tt220
type: Position
type: ExactPosition
position: 220
reference: Q64BR7-1

22859071192446766tt225
type: Position
type: ExactPosition
position: 225
reference: Q64BR7-1

22859071192446766tt232
type: Position
type: ExactPosition
position: 232
reference: Q64BR7-1

22859071192446766tt234
type: Position
type: ExactPosition
position: 234
reference: Q64BR7-1

22859071192446766tt254
type: Position
type: ExactPosition
position: 254
reference: Q64BR7-1

22859071192446766tt257
type: Position
type: ExactPosition
position: 257
reference: Q64BR7-1

22859071192446766tt270
type: Position
type: ExactPosition
position: 270
reference: Q64BR7-1

22859071192446766tt273
type: Position
type: ExactPosition
position: 273
reference: Q64BR7-1

22859071192446766tt287
type: Position
type: ExactPosition
position: 287
reference: Q64BR7-1

22859071192446766tt290
type: Position
type: ExactPosition
position: 290
reference: Q64BR7-1

22859071192446766tt300
type: Position
type: ExactPosition
position: 300
reference: Q64BR7-1

22859071192446766tt304
type: Position
type: ExactPosition
position: 304
reference: Q64BR7-1

22859071192446766tt315
type: Position
type: ExactPosition
position: 315
reference: Q64BR7-1

22859071192446766tt317
type: Position
type: ExactPosition
position: 317
reference: Q64BR7-1

22859071192446766tt327
type: Position
type: ExactPosition
position: 327
reference: Q64BR7-1

1
subclass of: CHEBI_18420

2
subclass of: CHEBI_18420

substrate

Q64BR7-1
type: Simple Sequence
modified: 2004-10-25
version: 1
mass: 36857
value: MEILHDEDVDDSILRDKTIAVMGYGAQGDAQANCLKDSGINVVIGETEILGGNKNPSWEKAKEDGFEVLPIDKAAEKGDVVHILLPDEVQPAIYENQIKPQLKAGKALCFSHGFNICFKRIVPPEDVDVIMVAPKAPGTEERKAYLEGFGVPGLVAVKQNPSGEAREVALAMTKAMHWTKAGILECTFEQETYEDLFGEQCVLCGGLVELMRNGFEVLVEAGYPPEMAYFECVHEMKLIVDLVWQGGIKRMAEVISNTAEYGMWAVGHQIIGPEVKEKMKEALKRVENGEFANEWVDEYKRGIPFLKASREKMGEHQVETVGAEIRKLFAQK

http://purl.uniprot.org/uniprot/Q64BR7#attribution-46641CFDA7575ABE0D4782FFBF747EC2
manual: true
evidence: ECO_0000250
source: K4LVZ1

http://purl.uniprot.org/uniprot/Q64BR7#attribution-EFC9D6CFD275FE14F70EFB5A18A2BCD5
manual: true
evidence: ECO_0000255
source: MF_00435

http://purl.uniprot.org/uniprot/Q64BR7#attribution-051F2B6E64DE62B0815A210AF1C5CC6C
manual: true
evidence: ECO_0000255
source: PRU01197

http://purl.uniprot.org/uniprot/Q64BR7#attribution-0D76E50A9E60CC6C81DCDA26FE0CFB20
manual: true
evidence: ECO_0000255
source: PRU01198

http://purl.uniprot.org/uniprot/Q64BR7#attribution-97FF8F34B009500EB88EFFE50C0442CE
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/25849365

http://purl.uniprot.org/uniprot/Q64BR7#attribution-A15AAB1881E8FBBC523E202663C5FF5D
manual: true
evidence: ECO_0000303
source: http://purl.uniprot.org/citations/25849365

http://purl.uniprot.org/uniprot/Q64BR7#attribution-665FB7CF20E143BCC8AF01BE31ADD379
manual: true
evidence: ECO_0000312
source: AAU83160.1