Q4PNI0
type: Protein
reviewed: true
created: 2011-05-31
modified: 2023-09-13
version: 38
mnemonic: BLH_UNCMB
citation: http://purl.uniprot.org/citations/16008504
citation: http://purl.uniprot.org/citations/19366683
citation: http://purl.uniprot.org/citations/20229064
recommended name: SIP2891119CF41777DB
enzyme: http://purl.uniprot.org/enzyme/1.13.11.63
organism: http://purl.uniprot.org/taxonomy/331677
annotation: SIP60B4A71EC81A3FBE
annotation: SIPB2636D5C01F33EA9
annotation: SIP9AE7B77F89250546
annotation: SIP3F1EAEA8CC9C1D5A
annotation: SIPE14ED3EBF85BBEE4
annotation: SIPB1B1C0FF2C7F9865
annotation: SIP6498E601863694AD
annotation: SIP7152A79A3564E4C4
annotation: SIP3F1385575605BEBC
annotation: SIP393CDEE4D52E7786
annotation: PRO_0000408499
annotation: SIPE96CBE0471DFE78C
annotation: SIPFCEB86DAF5076498
annotation: SIP22306EE89B37B840
annotation: SIPF36243C869A080AC
annotation: SIP1FE32CBE3D451B83
annotation: SIP074AF15CD2F86394
annotation: SIP3FD426AF9647C3AC
annotation: SIP3E0DD44E0993A018
annotation: SIP730243B5AF11DAEF
annotation: SIP1692452FEDC494E8
annotation: SIP4A6B91A6AB0E22D0
annotation: SIPB4622A16DD598CDD
annotation: SIP0D41E7588BCC59B6
annotation: SIP345A5EF97B9C5CB1
annotation: SIPA8B981E5292CA3D0
annotation: SIP60CDD4B5AD26036B
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/1003
classified with: http://purl.uniprot.org/keywords/223
classified with: http://purl.uniprot.org/keywords/408
classified with: http://purl.uniprot.org/keywords/479
classified with: http://purl.uniprot.org/keywords/1133
classified with: GO_0005886
classified with: GO_0003834
classified with: GO_0010436
classified with: GO_0005506
classified with: GO_0016121
classified with: GO_0042574
sequence: Q4PNI0-1

Q4PNI0-citation-16008504
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

Q4PNI0-citation-19366683
type: Citation Statement
scope: FUNCTION
scope: CATALYTIC ACTIVITY
scope: SUBSTRATE SPECIFICITY
scope: COFACTOR
scope: BIOPHYSICOCHEMICAL PROPERTIES
scope: SUBUNIT
scope: METAL-BINDING SITES
scope: REACTION MECHANISM
scope: MUTAGENESIS OF HIS-21; HIS-78; HIS-188 AND HIS-192

Q4PNI0-citation-20229064
type: Citation Statement
scope: BIOTECHNOLOGY

http://purl.uniprot.org/brenda/1.13.11.63
type: Resource
database: BRENDA
comment: 3186

MF_02093
type: Resource
database: HAMAP
comment: Beta_carotene_diox
signature sequence match: HAMAP MF 02093 match 1

IPR022270
type: Resource
database: InterPro
comment: Blh_diox

TIGR03753
type: Resource
database: NCBIfam
comment: blh_monoox
signature sequence match: NCBIfam TIGR03753 match 1

PF15461
type: Resource
database: Pfam
comment: BCD
signature sequence match: Pfam PF15461 match 1

SIP2891119CF41777DB
type: Structured Name
full name: Beta-carotene 15,15'-dioxygenase
ec name: 1.13.11.63

blh
type: Gene

SIP60B4A71EC81A3FBE
type: Function Annotation
comment: Catalyzes the cleavage of beta-carotene at its central double bond (15,15') to yield two molecules of all-trans-retinal. Exhibits the highest activity for beta-carotene, followed by beta-cryptoxanthin, beta-apo-4'-carotenal, alpha-carotene, and gamma-carotene in decreasing order, but has no activity on beta-apo-8'-carotenal, beta-apo-12'-carotenal, lutein, zeaxanthin, or lycopene, suggesting that the presence of one unsubstituted beta-ionone ring in a substrate with a molecular weight greater than C35 seems to be essential for enzyme activity.

SIP9AE7B77F89250546
type: Cofactor Annotation
comment: Binds 1 Fe(2+) ion per subunit.
cofactor: CHEBI_29033

SIP3F1EAEA8CC9C1D5A
type: Kinetics Annotation
comment: The kcat is 4.5-fold higher with beta-carotene than with beta-cryptoxanthin as substrate.
measured affinity: 37 uM for beta-carotene
measured affinity: 29 uM for beta-cryptoxanthin
measured affinity: 147 uM for beta-apo-4'-carotenal
measured affinity: 169 uM for alpha-carotene
measured affinity: 382 uM for gamma-carotene

SIPE14ED3EBF85BBEE4
type: PH Dependence Annotation
comment: Optimum pH is 8.0.

SIPB1B1C0FF2C7F9865
type: Temperature Dependence Annotation
comment: Optimum temperature is 40 degrees Celsius. Above this temperature, the enzyme activity decreases significantly, exhibiting 66% of the maximum activity at 50 degrees Celsius. Below 40 degrees Celsius, the enzyme activity decreases with decreasing temperature, exhibiting 15% of the maximum activity at 25 degrees Celsius. The half-lives of the enzyme at 35, 40, 45, 50, and 55 degrees Celsius are 17.6, 15.0, 12.5, 6.1, and 1.5 hours, respectively.

SIP6498E601863694AD
type: Subunit Annotation
comment: Homodimer.

SIP3F1385575605BEBC
type: Biotechnology Annotation
comment: Could be used for high retinal production.

SIP393CDEE4D52E7786
type: Similarity Annotation
comment: Belongs to the Brp/Blh beta-carotene diooxygenase family.

PRO_0000408499
type: Chain Annotation
comment: Beta-carotene 15,15'-dioxygenase
mass: 31037
range: 22856992629010478tt1tt275

22856992629010478tt1
type: Position
type: ExactPosition
position: 1
reference: Q4PNI0-1

22856992629010478tt275
type: Position
type: ExactPosition
position: 275
reference: Q4PNI0-1

SIPE96CBE0471DFE78C
type: Transmembrane Annotation
comment: Helical
range: 22856992629010478tt1tt21

22856992629010478tt21
type: Position
type: ExactPosition
position: 21
reference: Q4PNI0-1

SIPFCEB86DAF5076498
type: Transmembrane Annotation
comment: Helical
range: 22856992629010478tt36tt56

22856992629010478tt36
type: Position
type: ExactPosition
position: 36
reference: Q4PNI0-1

22856992629010478tt56
type: Position
type: ExactPosition
position: 56
reference: Q4PNI0-1

SIP22306EE89B37B840
type: Transmembrane Annotation
comment: Helical
range: 22856992629010478tt57tt77

22856992629010478tt57
type: Position
type: ExactPosition
position: 57
reference: Q4PNI0-1

22856992629010478tt77
type: Position
type: ExactPosition
position: 77
reference: Q4PNI0-1

SIPF36243C869A080AC
type: Transmembrane Annotation
comment: Helical
range: 22856992629010478tt91tt111

22856992629010478tt91
type: Position
type: ExactPosition
position: 91
reference: Q4PNI0-1

22856992629010478tt111
type: Position
type: ExactPosition
position: 111
reference: Q4PNI0-1

SIP1FE32CBE3D451B83
type: Transmembrane Annotation
comment: Helical
range: 22856992629010478tt127tt147

22856992629010478tt127
type: Position
type: ExactPosition
position: 127
reference: Q4PNI0-1

22856992629010478tt147
type: Position
type: ExactPosition
position: 147
reference: Q4PNI0-1

SIP074AF15CD2F86394
type: Transmembrane Annotation
comment: Helical
range: 22856992629010478tt160tt180

22856992629010478tt160
type: Position
type: ExactPosition
position: 160
reference: Q4PNI0-1

22856992629010478tt180
type: Position
type: ExactPosition
position: 180
reference: Q4PNI0-1

SIP3FD426AF9647C3AC
type: Transmembrane Annotation
comment: Helical
range: 22856992629010478tt210tt230

22856992629010478tt210
type: Position
type: ExactPosition
position: 210
reference: Q4PNI0-1

22856992629010478tt230
type: Position
type: ExactPosition
position: 230
reference: Q4PNI0-1

SIP3E0DD44E0993A018
type: Transmembrane Annotation
comment: Helical
range: 22856992629010478tt244tt264

22856992629010478tt244
type: Position
type: ExactPosition
position: 244
reference: Q4PNI0-1

22856992629010478tt264
type: Position
type: ExactPosition
position: 264
reference: Q4PNI0-1

22856992629010478tt78
type: Position
type: ExactPosition
position: 78
reference: Q4PNI0-1

22856992629010478tt188
type: Position
type: ExactPosition
position: 188
reference: Q4PNI0-1

22856992629010478tt192
type: Position
type: ExactPosition
position: 192
reference: Q4PNI0-1

SIP0D41E7588BCC59B6
type: Mutagenesis Annotation
comment: 3% of wild-type activity.
substitution: A
range: 22856992629010478tt21tt21

SIP345A5EF97B9C5CB1
type: Mutagenesis Annotation
comment: Loss of activity.
substitution: A
range: 22856992629010478tt78tt78

SIPA8B981E5292CA3D0
type: Mutagenesis Annotation
comment: Loss of activity.
substitution: A
range: 22856992629010478tt188tt188

SIP60CDD4B5AD26036B
type: Mutagenesis Annotation
comment: 5% of wild-type activity.
substitution: A
range: 22856992629010478tt192tt192

Q4PNI0-1
type: Simple Sequence
modified: 2005-07-19
version: 1
mass: 31037
value: MGLMLIDWCALALVVFIGLPHGALDAAISFSMISSAKRIARLAGILLIYLLLATAFFLIWYQLPAFSLLIFLLISIIHFGMADFNASPSKLKWPHIIAHGGVVTVWLPLIQKNEVTKLFSILTNGPTPILWDILLIFFLCWSIGVCLHTYETLRSKHYNIAFELIGLIFLAWYAPPLVTFATYFCFIHSRRHFSFVWKQLQHMSSKKMMIGSAIILSCTSWLIGGGIYFFLNSKMIASEAALQTVFIGLAALTVPHMILIDFIFRPHSSRIKIKN

http://purl.uniprot.org/uniprot/Q4PNI0#attribution-43EEA6850A63D0D4DC7ABC5617BA606F
creator: UniProtKB
manual: true
evidence: ECO_0000314
source: http://purl.uniprot.org/citations/19366683

http://purl.uniprot.org/uniprot/Q4PNI0#attribution-3ADDF0BE78928AA31EC8FD83D770D15E
manual: true
evidence: ECO_0000255
source: MF_02093

http://purl.uniprot.org/uniprot/Q4PNI0#attribution-8E5D3EB14D6CE043382A03174BA12552
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/19366683

http://purl.uniprot.org/uniprot/Q4PNI0#attribution-62EDBCA62470F5D49C3998D0A2AF02B3
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/20229064

http://purl.uniprot.org/uniprot/Q4PNI0#attribution-080ED3982FEC43F7C43A0689717F3D20
manual: true
evidence: ECO_0000305

http://purl.uniprot.org/uniprot/Q4PNI0#attribution-2DB4A841087ED15A1DEB1C3AB29220A1
manual: true
evidence: ECO_0000305
source: http://purl.uniprot.org/citations/19366683

A0A2I9
type: Protein
reviewed: true
created: 2018-11-07
modified: 2023-09-13
version: 46
mnemonic: CCSD_UNKP
citation: http://purl.uniprot.org/citations/17420004
citation: SIP18A8684F736C68F2
citation: SIPDDCAA58754644BAA
citation: http://purl.uniprot.org/citations/29796005
recommended name: SIPBD61EF2ADDC558DE
alternative name: SIPAC34A387899EA323
alternative name: SIPEAED42135EAD1C19
alternative name: SIP03209CE1A87669EF
enzyme: http://purl.uniprot.org/enzyme/4.1.1.-
enzyme: http://purl.uniprot.org/enzyme/4.1.1.29
organism: http://purl.uniprot.org/taxonomy/2725
annotation: SIP00F38684E636E708
annotation: SIPE0DB17256CE1F77F
annotation: SIP53E684FF49C7A3E6
annotation: SIPF76641CD6119E962
annotation: SIPE708EC6E7E6C9853
annotation: SIP4F07C29E8A4111FA
annotation: SIP02F611DEF461A530
annotation: SIP045F9D2A1EB2E342
annotation: SIPEC09F46C3DF8A8E6
annotation: PRO_0000445604
annotation: SIPAD882E2B5A52B6E7
annotation: SIP1B3EF646AB5720F7
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/210
classified with: GO_0010181
classified with: GO_0004632
classified with: GO_0004633
classified with: GO_0004782
classified with: GO_0015937
classified with: GO_0015941
sequence: A0A2I9-1

A0A2I9-citation-17420004
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
scope: FUNCTION
scope: CATALYTIC ACTIVITY

A0A2I9-citation-SIP18A8684F736C68F2
type: Citation Statement
scope: FUNCTION
scope: CATALYTIC ACTIVITY
scope: ACTIVITY REGULATION
scope: BIOPHYSICOCHEMICAL PROPERTIES
scope: MUTAGENESIS OF HIS-30 AND SER-113

A0A2I9-citation-SIPDDCAA58754644BAA
type: Citation Statement
scope: FUNCTION
scope: CATALYTIC ACTIVITY
scope: BIOPHYSICOCHEMICAL PROPERTIES

A0A2I9-citation-29796005
type: Citation Statement
scope: FUNCTION
scope: CATALYTIC ACTIVITY

http://purl.uniprot.org/brenda/4.1.1.29
type: Resource
database: BRENDA
comment: 12464

http://purl.uniprot.org/gene3d/3.40.50.10300
type: Resource
database: Gene3D
comment: CoaB-like
signature sequence match: Gene3D 3.40.50.10300 match 1

http://purl.uniprot.org/gene3d/3.40.50.1950
type: Resource
database: Gene3D
comment: Flavin prenyltransferase-like
signature sequence match: Gene3D 3.40.50.1950 match 1

MF_02225
type: Resource
database: HAMAP
comment: CoaBC
signature sequence match: HAMAP MF 02225 match 1

IPR035929
type: Resource
database: InterPro
comment: CoaB-like_sf

IPR005252
type: Resource
database: InterPro
comment: CoaBC

IPR007085
type: Resource
database: InterPro
comment: DNA/pantothenate-metab_flavo_C

IPR036551
type: Resource
database: InterPro
comment: Flavin_trans-like

IPR003382
type: Resource
database: InterPro
comment: Flavoprotein

TIGR00521
type: Resource
database: NCBIfam
comment: coaBC_dfp
signature sequence match: NCBIfam TIGR00521 match 1

PTHR14359
type: Resource
database: PANTHER
comment: HOMO-OLIGOMERIC FLAVIN CONTAINING CYS DECARBOXYLASE FAMILY
signature sequence match: PANTHER PTHR14359 match 1

PTHR14359:SF6
type: Resource
database: PANTHER
comment: PHOSPHOPANTOTHENOYLCYSTEINE DECARBOXYLASE
signature sequence match: PANTHER PTHR14359:SF6 match 1

PF04127
type: Resource
database: Pfam
comment: DFP
signature sequence match: Pfam PF04127 match 1

PF02441
type: Resource
database: Pfam
comment: Flavoprotein
signature sequence match: Pfam PF02441 match 1

SSF102645
type: Resource
database: SUPFAM
comment: CoaB-like
signature sequence match: SUPFAM SSF102645 match 1

SSF52507
type: Resource
database: SUPFAM
comment: Homo-oligomeric flavin-containing Cys decarboxylases, HFCD
signature sequence match: SUPFAM SSF52507 match 1

SIPBD61EF2ADDC558DE
type: Structured Name
full name: Cysteine/Cysteine sulfinic acid decarboxylase
ec name: 4.1.1.-
ec name: 4.1.1.29

SIPAC34A387899EA323
type: Structured Name
full name: Cysteine decarboxylase

SIPEAED42135EAD1C19
type: Structured Name
full name: L-cysteine sulfinate decarboxylase
short name: CSD

SIP03209CE1A87669EF
type: Structured Name
full name: Sulfinoalanine decarboxylase

undec1A
type: Gene

SIP00F38684E636E708
type: Function Annotation
comment: Catalyzes the decarboxylation of L-cysteine to cysteamine and of 3-sulfino-L-alanine (cysteine sulfinic acid) to hypotaurine (PubMed:17420004, Ref.2, Ref.3, PubMed:29796005). Also catalyzes the decarboxylation of various amino acids such as L-lysine, L-glutamate, L-asparaginate and L-proline (Ref.2). In vitro, shows highest activity with L-cysteine as substrate (Ref.2).

SIPF76641CD6119E962
type: Activity Regulation Annotation
comment: Slightly stimulated in the presence of 1 mM Mg(2+).

SIPE708EC6E7E6C9853
type: Kinetics Annotation
comment: kcat is 4.57 min(-1) with L-cysteine as substrate (Ref.2). kcat is 45.8 min(-1) with L-cysteine sulfinic acid as substrate (Ref.3).
measured affinity: 0.59 mM for L-cysteine
measured affinity: 1.557 mM for L-cysteine sulfinic acid

SIP4F07C29E8A4111FA
type: PH Dependence Annotation
comment: Optimum pH is 7.0.

SIP02F611DEF461A530
type: Temperature Dependence Annotation
comment: Optimum temperature is 35 degrees Celsius.

SIP045F9D2A1EB2E342
type: Similarity Annotation
comment: In the N-terminal section; belongs to the HFCD (homo-oligomeric flavin containing Cys decarboxylase) superfamily.

SIPEC09F46C3DF8A8E6
type: Similarity Annotation
comment: In the C-terminal section; belongs to the PPC synthetase family.

PRO_0000445604
type: Chain Annotation
comment: Cysteine/Cysteine sulfinic acid decarboxylase
mass: 38807
range: 18348930060859694tt1tt359

18348930060859694tt1
type: Position
type: ExactPosition
position: 1
reference: A0A2I9-1

18348930060859694tt359
type: Position
type: ExactPosition
position: 359
reference: A0A2I9-1

SIPAD882E2B5A52B6E7
type: Mutagenesis Annotation
comment: Loss of decarboxylase activity.
substitution: N
range: 18348930060859694tt30tt30

18348930060859694tt30
type: Position
type: ExactPosition
position: 30
reference: A0A2I9-1

SIP1B3EF646AB5720F7
type: Mutagenesis Annotation
comment: Loss of 80% of decarboxylase activity.
substitution: R
range: 18348930060859694tt113tt113

18348930060859694tt113
type: Position
type: ExactPosition
position: 113
reference: A0A2I9-1

A0A2I9-1
type: Simple Sequence
modified: 2006-11-14
version: 1
mass: 38807
value: MITPLTLATLSKNPILVDFFDPEDGRWNSHVDLGLWSDLYLIAPATANTIGKMAAGIADNLLLTSYLSARCPVFIAPAMDVDMLMHPATQRNLGILKSSGNHIIEPGSGELASGLTGKGRMAEPEEIVREVISFFSKKKITEKPLNGRRVFINAGPTIEPIDPVRFISNYSSGRMGIALADAAAAMGAEVTLVLGPVTLRPSSQDINVIDVRSAAEMKEASVEAFRECDIAILAAAVADFTPLTTSDKKIKRGSGEMVINLRPTEDIAAELGKMKKKNQLLVGFALETDDEITNASSKLKRKNLDMIVLNSLKDPGAGFGHETNRITIIDKSNNIDKFELKTKGEVAADIIRKILTLVH

http://purl.uniprot.org/uniprot/A0A2I9#attribution-35C77224460AFB5ED3D7AFCF2FB1E9DF
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/17420004

http://purl.uniprot.org/uniprot/A0A2I9#attribution-4AAA879AFF826AFA87C9CE3B22CF2F8B
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/29796005

http://purl.uniprot.org/uniprot/A0A2I9#attribution-D5DBEB150642C292F1740503DBC4CD7C
manual: true
evidence: ECO_0000269
source: SIP18A8684F736C68F2

http://purl.uniprot.org/uniprot/A0A2I9#attribution-5173EF971F42A47BF9ED60FF41305E9D
manual: true
evidence: ECO_0000269
source: SIPDDCAA58754644BAA

http://purl.uniprot.org/uniprot/A0A2I9#attribution-D5C625928AF66BF03EA89F033A9CC9BF
manual: true
evidence: ECO_0000303
source: http://purl.uniprot.org/citations/17420004

http://purl.uniprot.org/uniprot/A0A2I9#attribution-743AED1639A7DEA309A936B6F456B428
manual: true
evidence: ECO_0000303
source: http://purl.uniprot.org/citations/29796005

http://purl.uniprot.org/uniprot/A0A2I9#attribution-A6686D3C77C85BB0D95AA7F34940B347
manual: true
evidence: ECO_0000305

A0A0K2VM55
type: Protein
reviewed: true
created: 2017-03-15
modified: 2023-02-22
version: 17
mnemonic: CE15_UNKP
citation: SIPD9D51D0B4D0D3D6C
citation: http://purl.uniprot.org/citations/27433797
recommended name: SIPB665FA81B79FF881
enzyme: http://purl.uniprot.org/enzyme/3.1.1.-
organism: http://purl.uniprot.org/taxonomy/2725
annotation: SIP6061AF47E3E258C3
annotation: SIP8254DCD0C4AAC7F7
annotation: SIP6C9E29AF3A6A8C58
annotation: SIP5DBC958DF0FCF459
annotation: SIPC1733999A4B52CA5
annotation: SIP60AB3FEC71000362
annotation: SIP6339BCABBC9C44F2
annotation: SIP445948805FA96C44
annotation: SIP572C2F85CC7F2CF3
annotation: PRO_0000439191
annotation: SIPF5DEB725C162F9BD
annotation: SIP013BE744CDA826C3
annotation: SIP3EC7C8750BF9579D
annotation: SIP38B34D694DF42258
annotation: SIP1EA10E9D564A4BB6
annotation: SIP20D06D96C4F96DFE
annotation: SIPE5C0F5CEE364FC7C
annotation: SIP27FD0BAD6487ED4B
annotation: SIP572145B3BB81F2E6
annotation: SIP9C2DDDDC29DE0ADE
annotation: SIPC94B543EB7771CD3
annotation: SIP9EC65B7656304C11
annotation: SIPFEA7077178CF319F
annotation: SIP4733C27CAD64858D
annotation: SIP6416C2B43D2D8858
annotation: SIPD3028644D585EB79
annotation: SIPA4D716ABDBB3B14E
annotation: SIP3CA20A863C5E4239
annotation: SIPE6D0FF07093C9931
annotation: SIP78632B66EDE43E14
annotation: SIP69E1EBAEA89EDC8E
annotation: SIP913247B15BCAC42A
annotation: SIP588D7E7E71BA0356
annotation: SIPFA9C08B53494E495
annotation: SIP747F1358F9D8F6AC
annotation: SIP11B439923FEEB292
annotation: SIP4DBB09F01A546A6E
annotation: SIP0C605DFF01D73EE1
annotation: SIPF2F511F151E93151
annotation: SIPED36F6C9FA99DA47
annotation: SIP3C67CC34BE60E715
annotation: SIP7B5180E9182366AC
annotation: SIP7BE1534032D4B1FE
annotation: SIP5A5090AF49A6A4BC
annotation: SIPB2403681C93E67BA
annotation: SIP0347544128E8FDF0
annotation: SIP16F7F8E4BA653260
annotation: SIPCE73131BEF751678
annotation: SIP6F2555B04E46B0A2
annotation: SIP21134FA134E88697
annotation: SIPF79DFA6AB1586443
annotation: SIP7445D6F7D28E3A31
annotation: SIPDAD96E80BA7ABBC0
annotation: SIPCB78EFC58DD4552F
annotation: SIP54401AC16F1760C6
annotation: SIP129F67BAE8A044FB
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/2
classified with: http://purl.uniprot.org/keywords/574
classified with: http://purl.uniprot.org/keywords/624
classified with: http://purl.uniprot.org/keywords/719
classified with: http://purl.uniprot.org/keywords/732
classified with: GO_0042597
classified with: GO_0052689
classified with: GO_0000272
sequence: A0A0K2VM55-1

SIPD9D51D0B4D0D3D6C
type: Submission Citation
title: Three species of the Botryosphaeriales overlap on five unrelated trees in China, with a novel species.
author: Tian C.
author: Fan X.
date: 2014-06
submitted to: EMBL/GenBank/DDBJ

…
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

A0A0K2VM55-citation-27433797
type: Citation Statement
scope: FUNCTION
scope: ESTERASE ACTIVITY
scope: BIOPHYSICOCHEMICAL PROPERTIES
scope: SUBSTRATE SPECIFICITY
scope: COFACTOR
scope: ACTIVITY REGULATION
scope: SUBCELLULAR LOCATION
scope: 3D-STRUCTURE MODELING
scope: MUTAGENESIS OF 152-LYS-GLU-153; SER-268; CYS-291 AND HIS-409
scope: ACTIVE SITE

6EHN
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 1.90
chain sequence mapping: PDB 6EHN tt29tt427

PDB 6EHN tt29tt427
chain: A=29-427

unkp-CE15
type: Resource
database: ESTHER
comment: Glucuronoyl_esterase

http://purl.uniprot.org/gene3d/3.40.50.1820
type: Resource
database: Gene3D
comment: alpha/beta hydrolase
signature sequence match: Gene3D 3.40.50.1820 match 1

IPR029058
type: Resource
database: InterPro
comment: AB_hydrolase

SSF53474
type: Resource
database: SUPFAM
comment: alpha/beta-Hydrolases
signature sequence match: SUPFAM SSF53474 match 1

SIPB665FA81B79FF881
type: Structured Name
full name: Carbohydrate esterase MZ0003
ec name: 3.1.1.-

MZ0003
type: Gene

SIP6061AF47E3E258C3
type: Function Annotation
comment: Displays some glucuronoyl esterase activity in vitro, since it is able to hydrolyze methyl 4-O-methyl-D-glucopyranosyluronate, allyl D-glucuronate, benzyl D-glucuronate and D-glucuronic acid methyl ester. However, esters of glucuronic acid are probably not its biological substrate, as they are not present in the marine environment. Can also hydrolyze a range of other esters, including p-nitrophenyl acetate. More likely biologically-relevant substrates for MZ0003 and other marine bacterial CE15s are algal cell wall polysaccharides, as these would be readily available in this environment and could be used as energy sources.

SIP8254DCD0C4AAC7F7
type: Cofactor Annotation
comment: Does not require metal ions for activity.

SIP6C9E29AF3A6A8C58
type: Activity Regulation Annotation
comment: Is inhibited by PMSF and by NaF in vitro, which is consistent with the catalytic nucleophile being a serine.

SIP5DBC958DF0FCF459
type: Kinetics Annotation
comment: kcat is 8.9 sec(-1) with p-nitrophenyl acetate as substrate (in the absence of NaCl). kcat is 12.78 sec(-1) with p-nitrophenyl acetate as substrate (in the presence of 1M NaCl).
measured affinity: 0.71 mM for p-nitrophenyl acetate (in the absence of NaCl)
measured affinity: 0.97 mM for p-nitrophenyl acetate (in the presence of 1M NaCl)

SIPC1733999A4B52CA5
type: PH Dependence Annotation
comment: Optimum pH is 8.0. Shows more than 60% of its maximum activity in the pH range of 7.0-9.5. 20% of the activity is retained at low pH range and at pH 10.5, while no catalytic activity is found at pH 5.0 or 10.5.

SIP60AB3FEC71000362
type: Temperature Dependence Annotation
comment: Optimum temperature is 35 degrees Celsius with p-nitrophenyl acetate as substrate. Above 35 degrees Celsius, activity significantly decreases, while 30% of the maximum activity is recorded at 10 degrees Celsius.

SIP445948805FA96C44
type: Similarity Annotation
comment: Belongs to the carbohydrate esterase 15 (CE15) family.

-9218585095654255570tt1
type: Position
type: ExactPosition
position: 1
reference: A0A0K2VM55-1

-9218585095654255570tt25
type: Position
type: ExactPosition
position: 25
reference: A0A0K2VM55-1

PRO_0000439191
type: Chain Annotation
comment: Carbohydrate esterase MZ0003
mass: 45622
range: -9218585095654255570tt26tt432

-9218585095654255570tt26
type: Position
type: ExactPosition
position: 26
reference: A0A0K2VM55-1

-9218585095654255570tt432
type: Position
type: ExactPosition
position: 432
reference: A0A0K2VM55-1

SIPF5DEB725C162F9BD
type: Motif Annotation
comment: GXSYXG catalytic site motif
range: -9218585095654255570tt266tt271

-9218585095654255570tt266
type: Position
type: ExactPosition
position: 266
reference: A0A0K2VM55-1

-9218585095654255570tt271
type: Position
type: ExactPosition
position: 271
reference: A0A0K2VM55-1

SIP013BE744CDA826C3
type: Active Site Annotation
comment: Nucleophile
range: -9218585095654255570tt268tt268

-9218585095654255570tt268
type: Position
type: ExactPosition
position: 268
reference: A0A0K2VM55-1

SIP3EC7C8750BF9579D
type: Active Site Annotation
comment: Charge relay system
range: -9218585095654255570tt409tt409

-9218585095654255570tt409
type: Position
type: ExactPosition
position: 409
reference: A0A0K2VM55-1

-9218585095654255570tt272
type: Position
type: ExactPosition
position: 272
reference: A0A0K2VM55-1

-9218585095654255570tt359
type: Position
type: ExactPosition
position: 359
reference: A0A0K2VM55-1

SIP20D06D96C4F96DFE
type: Mutagenesis Annotation
comment: No effect on esterase activity.
substitution: AA
range: -9218585095654255570tt152tt153

-9218585095654255570tt152
type: Position
type: ExactPosition
position: 152
reference: A0A0K2VM55-1

-9218585095654255570tt153
type: Position
type: ExactPosition
position: 153
reference: A0A0K2VM55-1

SIPE5C0F5CEE364FC7C
type: Mutagenesis Annotation
comment: Loss of esterase activity.
substitution: A
range: -9218585095654255570tt268tt268

SIP27FD0BAD6487ED4B
type: Mutagenesis Annotation
comment: No effect on esterase activity.
substitution: A
range: -9218585095654255570tt291tt291

-9218585095654255570tt291
type: Position
type: ExactPosition
position: 291
reference: A0A0K2VM55-1

SIP572145B3BB81F2E6
type: Mutagenesis Annotation
comment: Loss of esterase activity.
substitution: A
range: -9218585095654255570tt409tt409

-9218585095654255570tt34
type: Position
type: ExactPosition
position: 34
reference: A0A0K2VM55-1

-9218585095654255570tt36
type: Position
type: ExactPosition
position: 36
reference: A0A0K2VM55-1

-9218585095654255570tt57
type: Position
type: ExactPosition
position: 57
reference: A0A0K2VM55-1

-9218585095654255570tt62
type: Position
type: ExactPosition
position: 62
reference: A0A0K2VM55-1

-9218585095654255570tt64
type: Position
type: ExactPosition
position: 64
reference: A0A0K2VM55-1

-9218585095654255570tt75
type: Position
type: ExactPosition
position: 75
reference: A0A0K2VM55-1

-9218585095654255570tt87
type: Position
type: ExactPosition
position: 87
reference: A0A0K2VM55-1

-9218585095654255570tt89
type: Position
type: ExactPosition
position: 89
reference: A0A0K2VM55-1

-9218585095654255570tt95
type: Position
type: ExactPosition
position: 95
reference: A0A0K2VM55-1

-9218585095654255570tt97
type: Position
type: ExactPosition
position: 97
reference: A0A0K2VM55-1

-9218585095654255570tt98
type: Position
type: ExactPosition
position: 98
reference: A0A0K2VM55-1

-9218585095654255570tt100
type: Position
type: ExactPosition
position: 100
reference: A0A0K2VM55-1

-9218585095654255570tt102
type: Position
type: ExactPosition
position: 102
reference: A0A0K2VM55-1

-9218585095654255570tt114
type: Position
type: ExactPosition
position: 114
reference: A0A0K2VM55-1

-9218585095654255570tt119
type: Position
type: ExactPosition
position: 119
reference: A0A0K2VM55-1

-9218585095654255570tt127
type: Position
type: ExactPosition
position: 127
reference: A0A0K2VM55-1

-9218585095654255570tt134
type: Position
type: ExactPosition
position: 134
reference: A0A0K2VM55-1

-9218585095654255570tt143
type: Position
type: ExactPosition
position: 143
reference: A0A0K2VM55-1

-9218585095654255570tt145
type: Position
type: ExactPosition
position: 145
reference: A0A0K2VM55-1

-9218585095654255570tt147
type: Position
type: ExactPosition
position: 147
reference: A0A0K2VM55-1

-9218585095654255570tt165
type: Position
type: ExactPosition
position: 165
reference: A0A0K2VM55-1

-9218585095654255570tt168
type: Position
type: ExactPosition
position: 168
reference: A0A0K2VM55-1

-9218585095654255570tt171
type: Position
type: ExactPosition
position: 171
reference: A0A0K2VM55-1

-9218585095654255570tt173
type: Position
type: ExactPosition
position: 173
reference: A0A0K2VM55-1

-9218585095654255570tt176
type: Position
type: ExactPosition
position: 176
reference: A0A0K2VM55-1

-9218585095654255570tt178
type: Position
type: ExactPosition
position: 178
reference: A0A0K2VM55-1

-9218585095654255570tt179
type: Position
type: ExactPosition
position: 179
reference: A0A0K2VM55-1

-9218585095654255570tt182
type: Position
type: ExactPosition
position: 182
reference: A0A0K2VM55-1

-9218585095654255570tt183
type: Position
type: ExactPosition
position: 183
reference: A0A0K2VM55-1

-9218585095654255570tt185
type: Position
type: ExactPosition
position: 185
reference: A0A0K2VM55-1

-9218585095654255570tt188
type: Position
type: ExactPosition
position: 188
reference: A0A0K2VM55-1

-9218585095654255570tt193
type: Position
type: ExactPosition
position: 193
reference: A0A0K2VM55-1

-9218585095654255570tt197
type: Position
type: ExactPosition
position: 197
reference: A0A0K2VM55-1

-9218585095654255570tt202
type: Position
type: ExactPosition
position: 202
reference: A0A0K2VM55-1

-9218585095654255570tt203
type: Position
type: ExactPosition
position: 203
reference: A0A0K2VM55-1

-9218585095654255570tt206
type: Position
type: ExactPosition
position: 206
reference: A0A0K2VM55-1

-9218585095654255570tt217
type: Position
type: ExactPosition
position: 217
reference: A0A0K2VM55-1

-9218585095654255570tt222
type: Position
type: ExactPosition
position: 222
reference: A0A0K2VM55-1

-9218585095654255570tt230
type: Position
type: ExactPosition
position: 230
reference: A0A0K2VM55-1

-9218585095654255570tt232
type: Position
type: ExactPosition
position: 232
reference: A0A0K2VM55-1

-9218585095654255570tt236
type: Position
type: ExactPosition
position: 236
reference: A0A0K2VM55-1

-9218585095654255570tt253
type: Position
type: ExactPosition
position: 253
reference: A0A0K2VM55-1

-9218585095654255570tt257
type: Position
type: ExactPosition
position: 257
reference: A0A0K2VM55-1

-9218585095654255570tt265
type: Position
type: ExactPosition
position: 265
reference: A0A0K2VM55-1

-9218585095654255570tt269
type: Position
type: ExactPosition
position: 269
reference: A0A0K2VM55-1

-9218585095654255570tt280
type: Position
type: ExactPosition
position: 280
reference: A0A0K2VM55-1

-9218585095654255570tt285
type: Position
type: ExactPosition
position: 285
reference: A0A0K2VM55-1

-9218585095654255570tt294
type: Position
type: ExactPosition
position: 294
reference: A0A0K2VM55-1

-9218585095654255570tt297
type: Position
type: ExactPosition
position: 297
reference: A0A0K2VM55-1

-9218585095654255570tt300
type: Position
type: ExactPosition
position: 300
reference: A0A0K2VM55-1

-9218585095654255570tt302
type: Position
type: ExactPosition
position: 302
reference: A0A0K2VM55-1

-9218585095654255570tt308
type: Position
type: ExactPosition
position: 308
reference: A0A0K2VM55-1

-9218585095654255570tt314
type: Position
type: ExactPosition
position: 314
reference: A0A0K2VM55-1

-9218585095654255570tt321
type: Position
type: ExactPosition
position: 321
reference: A0A0K2VM55-1

-9218585095654255570tt326
type: Position
type: ExactPosition
position: 326
reference: A0A0K2VM55-1

-9218585095654255570tt330
type: Position
type: ExactPosition
position: 330
reference: A0A0K2VM55-1

-9218585095654255570tt332
type: Position
type: ExactPosition
position: 332
reference: A0A0K2VM55-1

-9218585095654255570tt337
type: Position
type: ExactPosition
position: 337
reference: A0A0K2VM55-1

-9218585095654255570tt343
type: Position
type: ExactPosition
position: 343
reference: A0A0K2VM55-1

-9218585095654255570tt344
type: Position
type: ExactPosition
position: 344
reference: A0A0K2VM55-1

-9218585095654255570tt346
type: Position
type: ExactPosition
position: 346
reference: A0A0K2VM55-1

-9218585095654255570tt349
type: Position
type: ExactPosition
position: 349
reference: A0A0K2VM55-1

-9218585095654255570tt354
type: Position
type: ExactPosition
position: 354
reference: A0A0K2VM55-1

-9218585095654255570tt358
type: Position
type: ExactPosition
position: 358
reference: A0A0K2VM55-1

-9218585095654255570tt360
type: Position
type: ExactPosition
position: 360
reference: A0A0K2VM55-1

-9218585095654255570tt362
type: Position
type: ExactPosition
position: 362
reference: A0A0K2VM55-1

-9218585095654255570tt371
type: Position
type: ExactPosition
position: 371
reference: A0A0K2VM55-1

-9218585095654255570tt373
type: Position
type: ExactPosition
position: 373
reference: A0A0K2VM55-1

-9218585095654255570tt378
type: Position
type: ExactPosition
position: 378
reference: A0A0K2VM55-1

-9218585095654255570tt398
type: Position
type: ExactPosition
position: 398
reference: A0A0K2VM55-1

-9218585095654255570tt407
type: Position
type: ExactPosition
position: 407
reference: A0A0K2VM55-1

-9218585095654255570tt413
type: Position
type: ExactPosition
position: 413
reference: A0A0K2VM55-1

-9218585095654255570tt426
type: Position
type: ExactPosition
position: 426
reference: A0A0K2VM55-1

substrate

A0A0K2VM55-1
type: Simple Sequence
modified: 2015-11-11
version: 1
precursor: true
mass: 48457
value: MQRTCVLIVLIVTSTMWTPDPDVYAQPRGFNYDEAQVPKYTLPDPLVMVDGTKVTSAKQWNDKRRDEVQQLFEAYMYGKVPDGETELIFTDAKGERALGGAAIRKQVKISFGEKEDAPAMDLLIYLPADAKVRVPVFLGLNFHGNHTIHKDKEIWLTESWVRTNKKFGITKNKANELSRGVAAGRWQIEKAIAKGYGVATIYCGDIDPDFNFPSNGIQAYYYKKDQTIPEKGQWGTIAAWAFGLSCAMDYFETDTDIDHKKVAVLGHSRLGKTSLWAGAIDTRFALTISNCSGCGGAALSRRRFGETVRRINTSFPHWFCSRFHQYNDKEDKLPIDQHMLIALCAPRPVLINSATEDKWADPHGEFLAAQGADAVYRMLGTGGLDAKKWPEPNKLVKSTIGYHLRPGKHDVTARDWDVYIEFADHHMTGGAE

http://purl.uniprot.org/uniprot/A0A0K2VM55#attribution-0ADE65BAEC34C4E3B9C52C19D83B36B6
creator: UniProtKB
manual: true
evidence: ECO_0000314
source: http://purl.uniprot.org/citations/27433797

http://purl.uniprot.org/uniprot/A0A0K2VM55#attribution-150BBB5EC33E7F30BDBC03F0A2E6429D
manual: true
evidence: ECO_0000250
source: G2QJR6

http://purl.uniprot.org/uniprot/A0A0K2VM55#attribution-628A93785BCE70084CF622163024ED86
manual: true
evidence: ECO_0000255

http://purl.uniprot.org/uniprot/A0A0K2VM55#attribution-89072EC9DB8FFE2075099EB1920167E6
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/27433797

http://purl.uniprot.org/uniprot/A0A0K2VM55#attribution-095AC8C49784B7B15BB33821EA2FD490
manual: true
evidence: ECO_0000305
source: http://purl.uniprot.org/citations/27433797

http://purl.uniprot.org/uniprot/A0A0K2VM55#attribution-B3393B566875DF6862AFB8063A9C108C
manual: true
evidence: ECO_0000312
source: CDW92038.1

P56220
type: Protein
reviewed: true
created: 1998-07-15
modified: 2023-09-13
version: 111
mnemonic: DAPD_UNKP
old mnemonic: DAPD_MYCBO
citation: http://purl.uniprot.org/citations/8880935
citation: http://purl.uniprot.org/citations/19394346
citation: http://purl.uniprot.org/citations/9012664
citation: http://purl.uniprot.org/citations/9671504
citation: http://purl.uniprot.org/citations/11910040
recommended name: SIP598CA10675B5476B
alternative name: SIP655C004E95C185AF
enzyme: http://purl.uniprot.org/enzyme/2.3.1.117
organism: http://purl.uniprot.org/taxonomy/2725
annotation: SIPD888272AF3FDCC81
annotation: SIP2BD0F4554C71AB06
annotation: SIPA3DCFDD70269ACFE
annotation: SIPAD2D346A4FCE955D
annotation: SIP7EB1D8A1E4B348C5
annotation: SIP8BF56AD61EBF00A3
annotation: SIP319703FB6E85CFE8
annotation: PRO_0000196949
annotation: SIP87F17DD7993B2BE7
annotation: SIPA2123AF52F58A5EE
annotation: SIP45D350B9426C5315
annotation: SIP03D745F1A1BE1926
annotation: SIP5D6E252EC381F70D
annotation: SIPF1DEA23F707D38CB
annotation: SIPF28508CB0485429F
annotation: SIP094D580E87E6C4B8
annotation: SIPD5AC69090124733D
annotation: SIPD3F363199AE7D7E2
annotation: SIP389D2F7EEDFEB3C6
annotation: SIP8E95F1A14FA97EE1
annotation: SIP8999759E1BA879B2
annotation: SIP1BDF6046132EED59
annotation: SIPF344BDFB6EC3581A
annotation: SIPEB100716291AFB0B
annotation: SIP323A613A5F756B11
annotation: SIPD8B1C9BF15DF47AF
annotation: SIPC8148D6C57DBFE75
annotation: SIP1914F28D89C6A58C
annotation: SIP00350337A8E01A81
annotation: SIPBB7CE13BBCA4E5C3
annotation: SIPF19ED6D981BF3841
annotation: SIPE723DC80BB59F4D5
annotation: SIPCD5A436CFC57CE52
annotation: SIP4A74F654B81C504B
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/2
classified with: http://purl.uniprot.org/keywords/12
classified with: http://purl.uniprot.org/keywords/963
classified with: http://purl.uniprot.org/keywords/220
classified with: http://purl.uniprot.org/keywords/457
classified with: http://purl.uniprot.org/keywords/677
classified with: GO_0005737
classified with: GO_0008666
classified with: GO_0019877
classified with: GO_0009089
sequence: P56220-1

P56220-citation-8880935
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
scope: CRYSTALLIZATION

P56220-citation-19394346
type: Citation Statement
scope: DISCUSSION OF ORIGIN OF SEQUENCE

P56220-citation-9012664
type: Citation Statement
scope: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS)
scope: ACTIVITY REGULATION
scope: SUBUNIT

P56220-citation-9671504
type: Citation Statement
scope: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) IN COMPLEXES WITH ANALOGS SUBSTRATE

P56220-citation-11910040
type: Citation Statement
scope: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) IN COMPLEXES WITH ANALOGS SUBSTRATES

1KGQ
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.00
chain sequence mapping: PDB 1KGQ tt1tt274

PDB 1KGQ tt1tt274
chain: A=1-274

1KGT
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.30
chain sequence mapping: PDB 1KGT tt1tt274

PDB 1KGT tt1tt274
chain: A=1-274

1TDT
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.20
chain sequence mapping: PDB 1TDT tt1tt259

PDB 1TDT tt1tt259
chain: A/B/C=1-259

2TDT
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.00
chain sequence mapping: PDB 2TDT tt1tt274

PDB 2TDT tt1tt274
chain: A=1-274

3TDT
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.00
chain sequence mapping: PDB 3TDT tt1tt274

PDB 3TDT tt1tt274
chain: A=1-274

DB01992
type: Resource
database: DrugBank
comment: Coenzyme A

DB03134
type: Resource
database: DrugBank
comment: L-2-aminopimelic acid

DB01856
type: Resource
database: DrugBank
comment: Pimelic Acid

DB03905
type: Resource
database: DrugBank
comment: Succinamide-CoA

DB03699
type: Resource
database: DrugBank
comment: Succinyl-Coenzyme A

cd03350
type: Resource
database: CDD
comment: LbH_THP_succinylT
signature sequence match: CDD cd03350 match 1

http://purl.uniprot.org/gene3d/2.160.10.10
type: Resource
database: Gene3D
comment: Hexapeptide repeat proteins
signature sequence match: Gene3D 2.160.10.10 match 1

http://purl.uniprot.org/gene3d/1.10.166.10
type: Resource
database: Gene3D
comment: Tetrahydrodipicolinate-N-succinyltransferase, N-terminal domain
signature sequence match: Gene3D 1.10.166.10 match 1

MF_00811
type: Resource
database: HAMAP
comment: DapD
signature sequence match: HAMAP MF 00811 match 1

IPR005664
type: Resource
database: InterPro
comment: DapD_Trfase_Hexpep_rpt_fam

IPR001451
type: Resource
database: InterPro
comment: Hexapep

IPR018357
type: Resource
database: InterPro
comment: Hexapep_transf_CS

IPR023180
type: Resource
database: InterPro
comment: THP_succinylTrfase_dom1

IPR037133
type: Resource
database: InterPro
comment: THP_succinylTrfase_N_sf

IPR011004
type: Resource
database: InterPro
comment: Trimer_LpxA-like_sf

TIGR00965
type: Resource
database: NCBIfam
comment: dapD
signature sequence match: NCBIfam TIGR00965 match 1

PTHR19136:SF52
type: Resource
database: PANTHER
comment: 2,3,4,5-TETRAHYDROPYRIDINE-2,6-DICARBOXYLATE N-SUCCINYLTRANSFERASE
signature sequence match: PANTHER PTHR19136:SF52 match 1

PTHR19136
type: Resource
database: PANTHER
comment: MOLYBDENUM COFACTOR GUANYLYLTRANSFERASE
signature sequence match: PANTHER PTHR19136 match 1

PF14602
type: Resource
database: Pfam
comment: Hexapep_2
signature sequence match: Pfam PF14602 match 1

PF14805
type: Resource
database: Pfam
comment: THDPS_N_2
signature sequence match: Pfam PF14805 match 1

SSF51161
type: Resource
database: SUPFAM
comment: Trimeric LpxA-like enzymes
signature sequence match: SUPFAM SSF51161 match 1

PS00101
type: Resource
database: PROSITE
comment: HEXAPEP_TRANSFERASES
signature sequence match: PROSITE PS00101 match 1

SIP598CA10675B5476B
type: Structured Name
full name: 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase
ec name: 2.3.1.117

SIP655C004E95C185AF
type: Structured Name
full name: Tetrahydrodipicolinate N-succinyltransferase
short name: THDP succinyltransferase
short name: THP succinyltransferase
short name: Tetrahydropicolinate succinylase

dapD
type: Gene

SIP2BD0F4554C71AB06
type: Activity Regulation Annotation
comment: Inhibited by p-(chloromercuri)benzenesulfonic acid and cobalt.

SIPAD2D346A4FCE955D
type: Subunit Annotation
comment: Homotrimer.

SIP8BF56AD61EBF00A3
type: Similarity Annotation
comment: Belongs to the transferase hexapeptide repeat family.

SIP319703FB6E85CFE8
type: Caution Annotation
comment: Was originally thought to originate from Mycobacterium bovis (PubMed:8880935, PubMed:9012664, PubMed:9671504, PubMed:11910040). However, there is now convincing evidence that this is incorrect (PubMed:19394346). The source is unknown, but the sequence similarity suggests the protein is of enterobacterial origin.

PRO_0000196949
type: Chain Annotation
comment: 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase
mass: 29887
range: 22576505023508526tt1tt274

22576505023508526tt1
type: Position
type: ExactPosition
position: 1
reference: P56220-1

22576505023508526tt274
type: Position
type: ExactPosition
position: 274
reference: P56220-1

22576505023508526tt104
type: Position
type: ExactPosition
position: 104
reference: P56220-1

22576505023508526tt141
type: Position
type: ExactPosition
position: 141
reference: P56220-1

22576505023508526tt2
type: Position
type: ExactPosition
position: 2
reference: P56220-1

22576505023508526tt13
type: Position
type: ExactPosition
position: 13
reference: P56220-1

22576505023508526tt14
type: Position
type: ExactPosition
position: 14
reference: P56220-1

22576505023508526tt17
type: Position
type: ExactPosition
position: 17
reference: P56220-1

22576505023508526tt20
type: Position
type: ExactPosition
position: 20
reference: P56220-1

22576505023508526tt22
type: Position
type: ExactPosition
position: 22
reference: P56220-1

22576505023508526tt25
type: Position
type: ExactPosition
position: 25
reference: P56220-1

22576505023508526tt39
type: Position
type: ExactPosition
position: 39
reference: P56220-1

22576505023508526tt45
type: Position
type: ExactPosition
position: 45
reference: P56220-1

22576505023508526tt49
type: Position
type: ExactPosition
position: 49
reference: P56220-1

22576505023508526tt52
type: Position
type: ExactPosition
position: 52
reference: P56220-1

22576505023508526tt55
type: Position
type: ExactPosition
position: 55
reference: P56220-1

22576505023508526tt57
type: Position
type: ExactPosition
position: 57
reference: P56220-1

22576505023508526tt69
type: Position
type: ExactPosition
position: 69
reference: P56220-1

22576505023508526tt73
type: Position
type: ExactPosition
position: 73
reference: P56220-1

22576505023508526tt76
type: Position
type: ExactPosition
position: 76
reference: P56220-1

22576505023508526tt78
type: Position
type: ExactPosition
position: 78
reference: P56220-1

22576505023508526tt86
type: Position
type: ExactPosition
position: 86
reference: P56220-1

22576505023508526tt89
type: Position
type: ExactPosition
position: 89
reference: P56220-1

22576505023508526tt92
type: Position
type: ExactPosition
position: 92
reference: P56220-1

22576505023508526tt95
type: Position
type: ExactPosition
position: 95
reference: P56220-1

22576505023508526tt101
type: Position
type: ExactPosition
position: 101
reference: P56220-1

22576505023508526tt110
type: Position
type: ExactPosition
position: 110
reference: P56220-1

22576505023508526tt112
type: Position
type: ExactPosition
position: 112
reference: P56220-1

22576505023508526tt125
type: Position
type: ExactPosition
position: 125
reference: P56220-1

22576505023508526tt128
type: Position
type: ExactPosition
position: 128
reference: P56220-1

22576505023508526tt144
type: Position
type: ExactPosition
position: 144
reference: P56220-1

22576505023508526tt146
type: Position
type: ExactPosition
position: 146
reference: P56220-1

22576505023508526tt168
type: Position
type: ExactPosition
position: 168
reference: P56220-1

22576505023508526tt171
type: Position
type: ExactPosition
position: 171
reference: P56220-1

22576505023508526tt214
type: Position
type: ExactPosition
position: 214
reference: P56220-1

22576505023508526tt216
type: Position
type: ExactPosition
position: 216
reference: P56220-1

22576505023508526tt217
type: Position
type: ExactPosition
position: 217
reference: P56220-1

22576505023508526tt219
type: Position
type: ExactPosition
position: 219
reference: P56220-1

22576505023508526tt221
type: Position
type: ExactPosition
position: 221
reference: P56220-1

22576505023508526tt223
type: Position
type: ExactPosition
position: 223
reference: P56220-1

22576505023508526tt225
type: Position
type: ExactPosition
position: 225
reference: P56220-1

22576505023508526tt227
type: Position
type: ExactPosition
position: 227
reference: P56220-1

22576505023508526tt231
type: Position
type: ExactPosition
position: 231
reference: P56220-1

22576505023508526tt239
type: Position
type: ExactPosition
position: 239
reference: P56220-1

22576505023508526tt241
type: Position
type: ExactPosition
position: 241
reference: P56220-1

22576505023508526tt244
type: Position
type: ExactPosition
position: 244
reference: P56220-1

22576505023508526tt246
type: Position
type: ExactPosition
position: 246
reference: P56220-1

22576505023508526tt255
type: Position
type: ExactPosition
position: 255
reference: P56220-1

22576505023508526tt258
type: Position
type: ExactPosition
position: 258
reference: P56220-1

22576505023508526tt264
type: Position
type: ExactPosition
position: 264
reference: P56220-1

22576505023508526tt268
type: Position
type: ExactPosition
position: 268
reference: P56220-1

22576505023508526tt270
type: Position
type: ExactPosition
position: 270
reference: P56220-1

substrate

P56220-1
type: Simple Sequence
modified: 1998-12-15
version: 2
mass: 29887
value: MQQLQNVIESAFERRADITPANVDTVTREAVNQVIGLLDSGALRVAEKIDGQWVTHQWLKKAVLLSFRINDNKVMDGAETRYYDKVPMKFADYDEARFQKEGFRVVPPATVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSGGVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYLGQSTRIYDRETGEIHYGRVPAGSVVVSGNLPSKDGSYSLYCAVIVKKVDAKTRGKVGINELLRTID

http://purl.uniprot.org/uniprot/P56220#attribution-0F001B0237AE47E5270F7E9AC6FC1A67
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/9012664

http://purl.uniprot.org/uniprot/P56220#attribution-080ED3982FEC43F7C43A0689717F3D20
manual: true
evidence: ECO_0000305

http://purl.uniprot.org/uniprot/P56220#attribution-4ED4AAE678810D4BFAB6A47905195E03
manual: true
evidence: ECO_0000305
source: http://purl.uniprot.org/citations/19394346

P14657
type: Protein
reviewed: true
created: 1990-04-01
modified: 2023-05-03
version: 89
mnemonic: DHE4_UNKP
citation: http://purl.uniprot.org/citations/2690019
recommended name: SIP59B6866FF99DC8BD
enzyme: http://purl.uniprot.org/enzyme/1.4.1.4
organism: http://purl.uniprot.org/taxonomy/2725
annotation: SIP0A805C62C0C30875
annotation: SIP5265497B6AE54CEB
annotation: SIP0563F016D4B7E9C6
annotation: SIP591A009E34BFC887
annotation: SIP92D00B025FA4F16A
annotation: PRO_0000182778
annotation: SIP4C131DF27CBD53AE
annotation: SIPE526E11C3F04FCB8
annotation: SIPCB01B01E246EA0D1
annotation: SIPE47846577D203917
annotation: SIP79656B5AD1963053
annotation: SIPB851AF34841CE747
annotation: SIP7E4C28EA8741F1D3
annotation: SIPA1570E6B80D57190
annotation: SIP10E26E00D5658088
existence: Inferred from Homology Existence
classified with: http://purl.uniprot.org/keywords/521
classified with: http://purl.uniprot.org/keywords/560
classified with: GO_0005737
classified with: GO_0004354
classified with: GO_0006537
sequence: P14657-1

P14657-citation-2690019
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

cd05313
type: Resource
database: CDD
comment: NAD_bind_2_Glu_DH
signature sequence match: CDD cd05313 match 1

http://purl.uniprot.org/gene3d/1.10.285.10
type: Resource
database: Gene3D
comment: Glutamate Dehydrogenase, chain A, domain 3
signature sequence match: Gene3D 1.10.285.10 match 1
signature sequence match: Gene3D 1.10.285.10 match 2

http://purl.uniprot.org/gene3d/3.40.50.10860
type: Resource
database: Gene3D
comment: Leucine Dehydrogenase, chain A, domain 1
signature sequence match: Gene3D 3.40.50.10860 match 1

http://purl.uniprot.org/gene3d/3.40.50.720
type: Resource
database: Gene3D
comment: NAD(P)-binding Rossmann-like Domain
signature sequence match: Gene3D 3.40.50.720 match 1

IPR046346
type: Resource
database: InterPro
comment: Aminoacid_DH-like_N_sf

IPR006095
type: Resource
database: InterPro
comment: Glu/Leu/Phe/Val/Trp_DH

IPR006096
type: Resource
database: InterPro
comment: Glu/Leu/Phe/Val/Trp_DH_C

IPR006097
type: Resource
database: InterPro
comment: Glu/Leu/Phe/Val/Trp_DH_dimer

IPR033524
type: Resource
database: InterPro
comment: Glu/Leu/Phe/Val_DH_AS

IPR014362
type: Resource
database: InterPro
comment: Glu_DH

IPR036291
type: Resource
database: InterPro
comment: NAD(P)-bd_dom_sf

IPR033922
type: Resource
database: InterPro
comment: NAD_bind_Glu_DH

PTHR43571
type: Resource
database: PANTHER
comment: NADP-SPECIFIC GLUTAMATE DEHYDROGENASE 1-RELATED
signature sequence match: PANTHER PTHR43571 match 1

PTHR43571:SF1
type: Resource
database: PANTHER
comment: NADP-SPECIFIC GLUTAMATE DEHYDROGENASE 1-RELATED
signature sequence match: PANTHER PTHR43571:SF1 match 1

PF00208
type: Resource
database: Pfam
comment: ELFV_dehydrog
signature sequence match: Pfam PF00208 match 1

PF02812
type: Resource
database: Pfam
comment: ELFV_dehydrog_N
signature sequence match: Pfam PF02812 match 1

PIRSF000185
type: Resource
database: PIRSF
comment: Glu_DH
signature sequence match: PIRSF PIRSF000185 match 1

PR00082
type: Resource
database: PRINTS
comment: GLFDHDRGNASE

SM00839
type: Resource
database: SMART
comment: ELFV_dehydrog
signature sequence match: SMART SM00839 match 1

SSF53223
type: Resource
database: SUPFAM
comment: Aminoacid dehydrogenase-like, N-terminal domain
signature sequence match: SUPFAM SSF53223 match 1

SSF51735
type: Resource
database: SUPFAM
comment: NAD(P)-binding Rossmann-fold domains
signature sequence match: SUPFAM SSF51735 match 1

PS00074
type: Resource
database: PROSITE
comment: GLFV_DEHYDROGENASE
signature sequence match: PROSITE PS00074 match 1

SIP59B6866FF99DC8BD
type: Structured Name
full name: NADP-specific glutamate dehydrogenase
short name: NADP-GDH
ec name: 1.4.1.4

gdhA
type: Gene

SIP0A805C62C0C30875
type: Function Annotation
comment: Catalyzes the reversible oxidative deamination of glutamate to a-ketoglutarate and ammonia.

SIP0563F016D4B7E9C6
type: Subunit Annotation
comment: Homohexamer.

SIP591A009E34BFC887
type: Similarity Annotation
comment: Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

SIP92D00B025FA4F16A
type: Caution Annotation
comment: This sequence originates from an organism contaminating a Chlorella sorokiniana culture, probably a bacterium.

PRO_0000182778
type: Chain Annotation
comment: NADP-specific glutamate dehydrogenase
mass: 48490
range: 22572098454370094tt1tt446

22572098454370094tt1
type: Position
type: ExactPosition
position: 1
reference: P14657-1

22572098454370094tt446
type: Position
type: ExactPosition
position: 446
reference: P14657-1

SIP4C131DF27CBD53AE
type: Active Site Annotation
comment: Proton donor
range: 22572098454370094tt128tt128

22572098454370094tt128
type: Position
type: ExactPosition
position: 128
reference: P14657-1

22572098454370094tt92
type: Position
type: ExactPosition
position: 92
reference: P14657-1

22572098454370094tt113
type: Position
type: ExactPosition
position: 113
reference: P14657-1

22572098454370094tt116
type: Position
type: ExactPosition
position: 116
reference: P14657-1

22572098454370094tt167
type: Position
type: ExactPosition
position: 167
reference: P14657-1

22572098454370094tt211
type: Position
type: ExactPosition
position: 211
reference: P14657-1

22572098454370094tt242
type: Position
type: ExactPosition
position: 242
reference: P14657-1

22572098454370094tt379
type: Position
type: ExactPosition
position: 379
reference: P14657-1

SIP10E26E00D5658088
type: Site Annotation
comment: Important for catalysis
range: 22572098454370094tt168tt168

22572098454370094tt168
type: Position
type: ExactPosition
position: 168
reference: P14657-1

substrate

P14657-1
type: Simple Sequence
modified: 1990-04-01
version: 1
mass: 48490
value: MEYAVSVESFLSSLQRHNPHQPEYLQAVREVFTSLWPFIERNPAYREQALLERLVEPERIIQFRVSWVDDRGQVQVNRAFRVQFNSAIGPYKGGMRFHPSVNLSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSQGRIMRFCQALMTELYRHLGPDTDVPAGDIGDGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYFTDAMLQRHGLGFEGMRVAVSGSGNVAQYTIEKALELDARVITVSDSGGTLVDEDGFTTEKLAHLAEIKNQRYGRVADYAAERGLTYLAGQQPWNVPVDIALPCATQNELDLEAARVIRNGVKAVAEGANMPTTIQATDAFLDAGVLFAPGKAANAAGLATSGLEMAQNAARIGWRAEKVDVRLQHIMADIHHACVEYGGEGKQTHYVHGANIAGFVKVAEAMLAQGVL

http://purl.uniprot.org/uniprot/P14657#attribution-68097C5C9D39FEEE8BE013645ACBBDD4
creator: UniProtKB
manual: true
evidence: ECO_0000250

http://purl.uniprot.org/uniprot/P14657#attribution-609619A7DF3E53CE5E48EDEF8B8F9DD1
manual: true
evidence: ECO_0000250

http://purl.uniprot.org/uniprot/P14657#attribution-BADDF98EA065AD20B73080651D54FDD4
manual: true
evidence: ECO_0000255
source: PRU10011

http://purl.uniprot.org/uniprot/P14657#attribution-080ED3982FEC43F7C43A0689717F3D20
manual: true
evidence: ECO_0000305

A0A2P1BT06
type: Protein
reviewed: true
created: 2019-09-18
modified: 2023-02-22
version: 15
mnemonic: FCS1_UNKP
citation: SIP9F31B81774686579
citation: http://purl.uniprot.org/citations/30802241
recommended name: SIP4AEA1F3F550DA6A4
alternative name: SIP31BED4F6CB87E751
alternative name: SIP3E71F6F65FF5D677
enzyme: http://purl.uniprot.org/enzyme/6.2.1.34
organism: http://purl.uniprot.org/taxonomy/2725
annotation: SIP1DEF6DC2942F965A
annotation: SIPB783D7CD2AC30925
annotation: SIP164B609AA98F93A8
annotation: SIP4CA9A63F9A5ED47F
annotation: SIP180EAA4941F7316D
annotation: SIPB1C34FC1E87E6E7F
annotation: SIPDE9DF2F1FCF6A7FF
annotation: SIP04987992B7912EFD
annotation: SIPCC3C5AC4274CAE36
annotation: PRO_0000447891
annotation: SIPA670299FF9D8ED6D
annotation: SIP8C733385CCD566BA
annotation: SIPA60C2326B8633CE6
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/67
classified with: http://purl.uniprot.org/keywords/436
classified with: GO_0005524
classified with: GO_0046872
classified with: GO_0050563
sequence: A0A2P1BT06-1

SIP9F31B81774686579
type: Submission Citation
title: Uncultured organism feruloyl coenzyme A synthetase (fcs) gene.
author: Tomazetto G.
date: 2018-03
submitted to: EMBL/GenBank/DDBJ

…
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

A0A2P1BT06-citation-30802241
type: Citation Statement
scope: FUNCTION
scope: CATALYTIC ACTIVITY
scope: BIOPHYSICOCHEMICAL PROPERTIES
scope: SUBUNIT
scope: ATP-BINDING SITE
scope: BIOTECHNOLOGY

http://purl.uniprot.org/gene3d/3.30.1490.20
type: Resource
database: Gene3D
comment: ATP-grasp fold, A domain
signature sequence match: Gene3D 3.30.1490.20 match 1

http://purl.uniprot.org/gene3d/3.30.470.20
type: Resource
database: Gene3D
comment: ATP-grasp fold, B domain
signature sequence match: Gene3D 3.30.470.20 match 1

http://purl.uniprot.org/gene3d/3.40.50.261
type: Resource
database: Gene3D
comment: Succinyl-CoA synthetase domains
signature sequence match: Gene3D 3.40.50.261 match 1
signature sequence match: Gene3D 3.40.50.261 match 2

IPR011761
type: Resource
database: InterPro
comment: ATP-grasp

IPR013815
type: Resource
database: InterPro
comment: ATP_grasp_subdomain_1

IPR003781
type: Resource
database: InterPro
comment: CoA-bd

IPR032875
type: Resource
database: InterPro
comment: Succ_CoA_lig_flav_dom

IPR016102
type: Resource
database: InterPro
comment: Succinyl-CoA_synth-like

PTHR43334
type: Resource
database: PANTHER
comment: ACETATE--COA LIGASE [ADP-FORMING]
signature sequence match: PANTHER PTHR43334 match 1

PTHR43334:SF1
type: Resource
database: PANTHER
comment: ACETATE--COA LIGASE [ADP-FORMING]
signature sequence match: PANTHER PTHR43334:SF1 match 1

PF13549
type: Resource
database: Pfam
comment: ATP-grasp_5
signature sequence match: Pfam PF13549 match 1

PF13380
type: Resource
database: Pfam
comment: CoA_binding_2
signature sequence match: Pfam PF13380 match 1

PF13607
type: Resource
database: Pfam
comment: Succ_CoA_lig
signature sequence match: Pfam PF13607 match 1

SM00881
type: Resource
database: SMART
comment: CoA_binding
signature sequence match: SMART SM00881 match 1

SSF56059
type: Resource
database: SUPFAM
comment: Glutathione synthetase ATP-binding domain-like
signature sequence match: SUPFAM SSF56059 match 1

SSF52210
type: Resource
database: SUPFAM
comment: Succinyl-CoA synthetase domains
signature sequence match: SUPFAM SSF52210 match 1
signature sequence match: SUPFAM SSF52210 match 2

PS50975
type: Resource
database: PROSITE
comment: ATP_GRASP
signature sequence match: PROSITE PS50975 match 1

SIP4AEA1F3F550DA6A4
type: Structured Name
full name: Trans-feruloyl-CoA synthase FCS1
ec name: 6.2.1.34

SIP31BED4F6CB87E751
type: Structured Name
full name: Feruloyl coenzyme A synthetase

SIP3E71F6F65FF5D677
type: Structured Name
full name: Trans-feruloyl-CoA synthetase

FCS1
type: Gene
alternative label: Fcs

SIP1DEF6DC2942F965A
type: Function Annotation
comment: Catalyzes the formation of feruloyl-CoA, ADP and phosphate from ferulate, CoA and ATP.

SIP164B609AA98F93A8
type: Kinetics Annotation
measured affinity: 0.1 mM for ferulate

SIP4CA9A63F9A5ED47F
type: PH Dependence Annotation
comment: Optimum pH is 7.8.

SIP180EAA4941F7316D
type: Temperature Dependence Annotation
comment: Optimum temperature is 37 degrees Celsius.

SIPB1C34FC1E87E6E7F
type: Subunit Annotation
comment: Homodimer.

SIPDE9DF2F1FCF6A7FF
type: Biotechnology Annotation
comment: Catalyzes the activation of ferulate via CoA-thioesterification, a first enzymatic step for conversion of the lignin-derived ferulate into high value chemicals, such as vanillin and bioplastics.

SIP04987992B7912EFD
type: Similarity Annotation
comment: In the N-terminal section; belongs to the acetate CoA ligase alpha subunit family.

SIPCC3C5AC4274CAE36
type: Similarity Annotation
comment: In the C-terminal section; belongs to the acetate CoA ligase beta subunit family.

PRO_0000447891
type: Chain Annotation
comment: Trans-feruloyl-CoA synthase FCS1
mass: 74923
range: -9218575961593735122tt1tt707

-9218575961593735122tt1
type: Position
type: ExactPosition
position: 1
reference: A0A2P1BT06-1

-9218575961593735122tt707
type: Position
type: ExactPosition
position: 707
reference: A0A2P1BT06-1

SIPA670299FF9D8ED6D
type: Domain Extent Annotation
comment: ATP-grasp
range: -9218575961593735122tt498tt549

-9218575961593735122tt498
type: Position
type: ExactPosition
position: 498
reference: A0A2P1BT06-1

-9218575961593735122tt549
type: Position
type: ExactPosition
position: 549
reference: A0A2P1BT06-1

-9218575961593735122tt267
type: Position
type: ExactPosition
position: 267
reference: A0A2P1BT06-1

-9218575961593735122tt524
type: Position
type: ExactPosition
position: 524
reference: A0A2P1BT06-1

-9218575961593735122tt535
type: Position
type: ExactPosition
position: 535
reference: A0A2P1BT06-1

A0A2P1BT06-1
type: Simple Sequence
modified: 2018-05-23
version: 1
mass: 74923
value: MGERRFSNQQIDRLLRPKSVAVIGASDRKGALGATLLNNLVQYEFSGDIYPVNPKRDELLGLKVYHEVAELPEGIDCAVLAIPRPFVIDTVRQLAQRGCGAVVIYSAGFSEAGEEGMKDQLELAAIAAEYGMVIEGPNCLGCTNYVERVPLTFVETNMQTPPKGTRAVGIASQSGALAAVLATALHPRGLYVSSSVSTGNEAASGVEDYVEWLVDDEDTHVIAMYVESLRRPKAFIAAARRAHAAGKPIVMLHPGKSNKAQESAATHTGAMAGDYALMKTKLAREGVIFADTLEELADITEIALRCRALPGANMAVLGESGALRGLAFDIAEDIGLDLIHLDDDNSPALRAILPDFVPVSNPTDITALGLSEPEIYTKVLTALLEDERIGSVVASIIQSDPITSGIKFPHIIKVLDGGTFAKPLVFAGVDEGATVPKEYIDGLRKVGIPWFPSTERAYRAIARLADLSKRDLADNSGDPIVVPGLDAVSGVVPEYKAKELLRPLGIAFPPSQFAANAEAAAAAARAIGYPVVMKAQAAALGHKSDAGGVILNLKTDDEVRDAFARIYGNVEAYDRSIALDGVLIEKMGKMGTEMIVGAKNDPQWGPVVLAGFGGVTAEILKDVKLFTPEMDAAAVQRGLLELKQAPILKGYRGAPALDVAALAELIVQIGRVMAGNPSIREIDLNPVIIHPAGEGVAALDALMLVER

http://purl.uniprot.org/uniprot/A0A2P1BT06#attribution-CADB3F4FCCBCCABEB5D59191FE5F0AB3
manual: true
evidence: ECO_0000255
source: PRU00409

http://purl.uniprot.org/uniprot/A0A2P1BT06#attribution-AB8F7AA1DDFECCAEF3BF04D372845E1D
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/30802241

http://purl.uniprot.org/uniprot/A0A2P1BT06#attribution-3A0209FA179807DE75E3F19FC972564B
manual: true
evidence: ECO_0000303
source: http://purl.uniprot.org/citations/30802241

http://purl.uniprot.org/uniprot/A0A2P1BT06#attribution-57502D81BF90080BC7D76B7DA183E484
manual: true
evidence: ECO_0000303
source: SIP9F31B81774686579

http://purl.uniprot.org/uniprot/A0A2P1BT06#attribution-28AEECC9A05986C7F1A958E1FB525076
manual: true
evidence: ECO_0000305

http://purl.uniprot.org/uniprot/A0A2P1BT06#attribution-42D17A80E91394E6AD57701E570DD209
manual: true
evidence: ECO_0000305
source: http://purl.uniprot.org/citations/30802241

P0DV52
type: Protein
reviewed: true
created: 2022-05-25
modified: 2023-06-28
version: 5
mnemonic: GSDM_UNKP
citation: SIP9A1699B052A88F3B
citation: http://purl.uniprot.org/citations/35025633
recommended name: SIP4C68E5175B98EBE9
alternative name: SIP9621075F5A8DB360
component: SIPF57019A32C91DDF2
organism: http://purl.uniprot.org/taxonomy/2725
annotation: SIP724CC0286E82E0C5
annotation: SIPA10FEB131CD8D150
annotation: SIPB5F2B2991C6AF33D
annotation: SIP3870DBEC9560AC26
annotation: SIPFE0627739E027951
annotation: SIP49948B1AF8AED43B
annotation: SIP52058931E0746FEF
annotation: SIP2E81F547D5C8F4A0
annotation: SIPA6BD792A9AF80B5D
annotation: SIPF71197FE1FA142EC
annotation: SIPB679298A9BEE0D14
annotation: PRO_0000455574
annotation: PRO_0000455575
annotation: SIP5D24DFA1AE9ED9CB
annotation: SIPBC5EE298D1CF30EE
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/51
classified with: http://purl.uniprot.org/keywords/1003
classified with: http://purl.uniprot.org/keywords/963
classified with: http://purl.uniprot.org/keywords/1134
classified with: GO_0005737
classified with: GO_0005886
classified with: GO_0051607
sequence: P0DV52-1

SIP9A1699B052A88F3B
type: Submission Citation
title: Saline water microbial communities from Organic Lake, Antarctica - #987.
group: DOE Joint Genome Institute
date: 2019-01
submitted to: EMBL/GenBank/DDBJ

P0DV52-citation-SIP9A1699B052A88F3B
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]

P0DV52-citation-35025633
type: Citation Statement
scope: IDENTIFICATION
scope: FUNCTION
scope: SUBUNIT
scope: SUBCELLULAR LOCATION
scope: MASS SPECTROMETRY

SIP4C68E5175B98EBE9
type: Structured Name
full name: Gasdermin bGSDM
short name: bGSDM

SIP9621075F5A8DB360
type: Structured Name
full name: Bacterial gasdermin

SIPD5C453838954D691
type: Structured Name
full name: Gasdermin bGSDM, N-terminus

http://purl.uniprot.org/uniprot/P0DV52#gene-MD5E8EE61631722F3EC1F447CC5D21B6086
type: Gene
orf name: Ga0307981_100051429

SIP724CC0286E82E0C5
type: Function Annotation
comment: Precursor of a pore-forming protein involved in defense against bacteriophages (By similarity). Cleavage of this precursor by its dedicated protease releases the active moiety (gasdermin bGSDM, N-terminus) which inserts into membranes, forming pores and triggering cell death (PubMed:35025633). Expression of bGSDM and the neighboring protease gene (Ga0307981_100051430) is highly toxic in E.coli (PubMed:35025633).
sequence: PRO_0000455574

SIPA10FEB131CD8D150
type: Function Annotation
comment: Pore-forming protein that causes membrane permeabilization via a pyroptosis-like activity. This is the active form which makes ring-like pores with an interior pore diameter of 130-190 Angstroms, when integrated in liposomes.
sequence: PRO_0000455575

SIPB5F2B2991C6AF33D
type: Activity Regulation Annotation
comment: The full-length protein before cleavage is inactive: intramolecular interactions between the N-terminal domain and the C-terminal region mediate autoinhibition. The pyroptosis-like-inducing activity is carried by the released N-terminal domain (Gasdermin bGSDM, N-terminus).
sequence: PRO_0000455574

SIP3870DBEC9560AC26
type: Subunit Annotation
comment: Monomer.
sequence: PRO_0000455574

SIPFE0627739E027951
type: Subunit Annotation
comment: Forms large, probably homooligomeric ring-shaped pores when inserted in membranes.
sequence: PRO_0000455575

SIP52058931E0746FEF
type: Subcellular Location Annotation
comment: Upon proteolysis the protein forms membrane-associated puncta.
located in: SIPF1DB216A3393B2A4
sequence: PRO_0000455575

SIP2E81F547D5C8F4A0
type: Domain Annotation
comment: The N-terminus has marked structural similarity to the mammalian gasdermin N-terminal domain. The C-terminal region wraps around the twisted beta sheet core, probably stabilizing the inactive state.

SIPA6BD792A9AF80B5D
type: Mass Spectrometry Annotation
comment: Gasdermin bGSDM, N-terminus.
measured value: 28280.51
method: ESI

SIPF71197FE1FA142EC
type: Annotation
comment: Might be a Bacteriodetes scaffold.

SIPB679298A9BEE0D14
type: Similarity Annotation
comment: Belongs to the bacterial gasdermin family.

PRO_0000455574
type: Chain Annotation
comment: Gasdermin bGSDM
mass: 30335
range: 22571068199088686tt1tt266

22571068199088686tt1
type: Position
type: ExactPosition
position: 1
reference: P0DV52-1

22571068199088686tt266
type: Position
type: ExactPosition
position: 266
reference: P0DV52-1

PRO_0000455575
type: Chain Annotation
comment: Gasdermin bGSDM, N-terminus
mass: 28325
range: 22571068199088686tt1tt247

22571068199088686tt247
type: Position
type: ExactPosition
position: 247
reference: P0DV52-1

SIP5D24DFA1AE9ED9CB
type: Region Annotation
comment: C-terminal region
range: 22571068199088686tt248tt266

22571068199088686tt248
type: Position
type: ExactPosition
position: 248
reference: P0DV52-1

SIPBC5EE298D1CF30EE
type: Site Annotation
comment: Cleavage; by protease Ga0307981_100051430
range: 22571068199088686tt247tt248

P0DV52-1
type: Simple Sequence
modified: 2022-05-25
version: 1
mass: 30335
value: MIKYLQSHLEEQGYLFVTLPKPDLAPLQLLTEYKGHLEEYDGSLLDLFEPDGSPFPIRDRQLPNFSGQQLLQTDWSAGADLLHGLFKLFQQKEDKLKASLSGMKGLVLSFAYENIEEERVSEQALDNFLAGAMPKKEGFQRSVERLQDGELYVLTSVMRSNQFTVTIDCQREDQGKLEAAVAEIVDAHASIERKQSNSFSLQTEGEQAFVFACRAAQVLYNKKQWFQFWKKDKDGFRIEKREGMVVRGEEDFSVQPLQAPSGLLKL

http://purl.uniprot.org/uniprot/P0DV52#attribution-7682AEFBFEDB411AD88031430E651267
manual: true
evidence: ECO_0000250
source: A0A0S2DNG5

http://purl.uniprot.org/uniprot/P0DV52#attribution-AFA976D000EE968FDE301F833667B23F
manual: true
evidence: ECO_0000250
source: P0DV48

http://purl.uniprot.org/uniprot/P0DV52#attribution-0B651BC8E59F675277B344ADBF95177B
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/35025633

http://purl.uniprot.org/uniprot/P0DV52#attribution-4E01FC9EB5FA03BE1E86EE922952056B
manual: true
evidence: ECO_0000303
source: http://purl.uniprot.org/citations/35025633

http://purl.uniprot.org/uniprot/P0DV52#attribution-E3E4B13BE0C82296A2F42D68997EDEFB
manual: true
evidence: ECO_0000305

http://purl.uniprot.org/uniprot/P0DV52#attribution-D292D60BC11E5752911F750BA0BED40A
manual: true
evidence: ECO_0000305
source: http://purl.uniprot.org/citations/35025633

P0CI72
type: Protein
reviewed: true
created: 2011-01-11
modified: 2023-02-22
version: 27
mnemonic: IXPDE_UNKP
citation: http://purl.uniprot.org/citations/20415463
recommended name: SIPC97D68350897696B
enzyme: http://purl.uniprot.org/enzyme/3.5.4.11
organism: http://purl.uniprot.org/taxonomy/2725
annotation: SIPF739305C676C3343
annotation: SIP3033C7EEEC9FDE38
annotation: SIP6BDE397DCD028D1A
annotation: SIP1ED0DBA3A82A3574
annotation: PRO_0000403657
annotation: SIPAFA69E933A6CB805
annotation: SIP8DE7078EBDB29CD1
annotation: SIP1C5BE6A2867EB4F9
annotation: SIP18138FA219615C83
annotation: SIPE81AF846C50486E4
annotation: SIP719F45E41E9922AC
annotation: SIPFA763F4266F71D6A
annotation: SIP8D00EA74A7ED3F6E
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/2
classified with: http://purl.uniprot.org/keywords/378
classified with: http://purl.uniprot.org/keywords/479
classified with: http://purl.uniprot.org/keywords/862
classified with: GO_0046872
classified with: GO_0050228
sequence: P0CI72-1

P0CI72-citation-20415463
type: Citation Statement
scope: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) IN COMPLEX WITH ZINC IONS
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
scope: CATALYTIC ACTIVITY
scope: COFACTOR
scope: BIOPHYSICOCHEMICAL PROPERTIES

2PAJ
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.70
chain sequence mapping: PDB 2PAJ tt1tt482

PDB 2PAJ tt1tt482
chain: A=1-482

http://purl.uniprot.org/brenda/3.5.4.11
type: Resource
database: BRENDA
comment: 12464

cd01298
type: Resource
database: CDD
comment: ATZ_TRZ_like
signature sequence match: CDD cd01298 match 1

http://purl.uniprot.org/gene3d/3.20.20.140
type: Resource
database: Gene3D
comment: Metal-dependent hydrolases
signature sequence match: Gene3D 3.20.20.140 match 1

http://purl.uniprot.org/gene3d/2.30.40.10
type: Resource
database: Gene3D
comment: Urease, subunit C, domain 1
signature sequence match: Gene3D 2.30.40.10 match 1
signature sequence match: Gene3D 2.30.40.10 match 2

IPR006680
type: Resource
database: InterPro
comment: Amidohydro-rel

IPR011059
type: Resource
database: InterPro
comment: Metal-dep_hydrolase_composite

IPR032466
type: Resource
database: InterPro
comment: Metal_Hydrolase

PTHR43794:SF11
type: Resource
database: PANTHER
comment: AMIDOHYDRO-REL DOMAIN-CONTAINING PROTEIN
signature sequence match: PANTHER PTHR43794:SF11 match 1

PTHR43794
type: Resource
database: PANTHER
comment: AMINOHYDROLASE SSNA-RELATED
signature sequence match: PANTHER PTHR43794 match 1

PF01979
type: Resource
database: Pfam
comment: Amidohydro_1
signature sequence match: Pfam PF01979 match 1

SSF51338
type: Resource
database: SUPFAM
comment: Composite domain of metallo-dependent hydrolases
signature sequence match: SUPFAM SSF51338 match 1

SSF51556
type: Resource
database: SUPFAM
comment: Metallo-dependent hydrolases
signature sequence match: SUPFAM SSF51556 match 1

SIPC97D68350897696B
type: Structured Name
full name: Isoxanthopterin deaminase
ec name: 3.5.4.11

SIP3033C7EEEC9FDE38
type: Cofactor Annotation
comment: Binds 1 zinc ion per subunit.
cofactor: CHEBI_29105

SIP6BDE397DCD028D1A
type: Kinetics Annotation
measured affinity: 8 uM for isoxanthopterin

SIP1ED0DBA3A82A3574
type: Similarity Annotation
comment: Belongs to the metallo-dependent hydrolases superfamily. ATZ/TRZ family.

PRO_0000403657
type: Chain Annotation
comment: Isoxanthopterin deaminase
mass: 51752
range: 22571063686148654tt1tt482

22571063686148654tt1
type: Position
type: ExactPosition
position: 1
reference: P0CI72-1

22571063686148654tt482
type: Position
type: ExactPosition
position: 482
reference: P0CI72-1

22571063686148654tt74
type: Position
type: ExactPosition
position: 74
reference: P0CI72-1

22571063686148654tt76
type: Position
type: ExactPosition
position: 76
reference: P0CI72-1

22571063686148654tt79
type: Position
type: ExactPosition
position: 79
reference: P0CI72-1

22571063686148654tt246
type: Position
type: ExactPosition
position: 246
reference: P0CI72-1

22571063686148654tt249
type: Position
type: ExactPosition
position: 249
reference: P0CI72-1

22571063686148654tt283
type: Position
type: ExactPosition
position: 283
reference: P0CI72-1

22571063686148654tt334
type: Position
type: ExactPosition
position: 334
reference: P0CI72-1

substrate

P0CI72-1
type: Simple Sequence
modified: 2011-01-11
version: 1
mass: 51752
value: MTTYDTQPSTLIRNAAAIMTGGRGTADDPSRVPGPDIRIVGDTIDAIGALAPRPGETIVDATDCVIYPAWVNTHHHLFQSLLKGEPAGLDATLTPWLAATPYRFRALFDERRFRLAARIGLIELARSGCATVADHNYVYYPGMPFDSSAILFEEAEKLGLRFVLLRGGATQTRQLEADLPTALRPETLDAYVADIERLAARYHDASPRAMRRVVMAPTTVLYSISPREMRETAAVARRLGLRMHSHLSETVGYQDSAYSMYGKSPVAFCGEHDWLGSDVWYAHLVKVDADEIALLAQTGTGVAHCPQSNGRLGSGICPVREMADAGVPVSIGVDGAASNEAADMISEVHMTWLAQRARLGMLAQPAYRGGSFEGGAGAASIAEVIHWGTAGGARVMGLDEVGKVAVGYAADIAVYRLDDPRYFGLHDPAIGPVASGGRPSVMALFSAGKRVVVDDLIEGVDIKELGGEARRVVRELLREVVV

http://purl.uniprot.org/uniprot/P0CI72#attribution-609619A7DF3E53CE5E48EDEF8B8F9DD1
manual: true
evidence: ECO_0000250

http://purl.uniprot.org/uniprot/P0CI72#attribution-D48F7DB54269566FBE08FD53FCF322E6
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/20415463

http://purl.uniprot.org/uniprot/P0CI72#attribution-080ED3982FEC43F7C43A0689717F3D20
manual: true
evidence: ECO_0000305

Q6RWK4
type: Protein
reviewed: true
created: 2013-05-01
modified: 2023-02-22
version: 45
mnemonic: NITR2_UNKP
citation: http://purl.uniprot.org/citations/15066841
citation: http://purl.uniprot.org/citations/12148986
recommended name: SIPF704FEA34C90239E
alternative name: SIP37B52E5BEE552DBE
enzyme: http://purl.uniprot.org/enzyme/3.5.5.1
organism: http://purl.uniprot.org/taxonomy/2725
annotation: SIP4A9AE8CF626FEE40
annotation: SIP15B3D60A6F2E0290
annotation: SIP926AEAEAFF11C449
annotation: PRO_0000422218
annotation: SIP54CAA9552DD4CB5B
annotation: SIP2FE167B904585D72
annotation: SIP019526BCEF7A03CC
annotation: SIP21E66FBF39DA6822
existence: Inferred from Homology Existence
classified with: http://purl.uniprot.org/keywords/378
classified with: GO_0080061
classified with: GO_0006807
sequence: Q6RWK4-1

Q6RWK4-citation-15066841
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

Q6RWK4-citation-12148986
type: Citation Statement
scope: FUNCTION

cd07564
type: Resource
database: CDD
comment: nitrilases_CHs
signature sequence match: CDD cd07564 match 1

http://purl.uniprot.org/gene3d/3.60.110.10
type: Resource
database: Gene3D
comment: Carbon-nitrogen hydrolase
signature sequence match: Gene3D 3.60.110.10 match 1

IPR003010
type: Resource
database: InterPro
comment: C-N_Hydrolase

IPR036526
type: Resource
database: InterPro
comment: C-N_Hydrolase_sf

IPR000132
type: Resource
database: InterPro
comment: Nitrilase/CN_hydratase_CS

IPR044149
type: Resource
database: InterPro
comment: Nitrilases_CHs

PTHR46044:SF1
type: Resource
database: PANTHER
comment: CN HYDROLASE DOMAIN-CONTAINING PROTEIN
signature sequence match: PANTHER PTHR46044:SF1 match 1

PTHR46044
type: Resource
database: PANTHER
comment: NITRILASE
signature sequence match: PANTHER PTHR46044 match 1

PF00795
type: Resource
database: Pfam
comment: CN_hydrolase
signature sequence match: Pfam PF00795 match 1

SSF56317
type: Resource
database: SUPFAM
comment: Carbon-nitrogen hydrolase
signature sequence match: SUPFAM SSF56317 match 1

PS50263
type: Resource
database: PROSITE
comment: CN_HYDROLASE
signature sequence match: PROSITE PS50263 match 1

PS00920
type: Resource
database: PROSITE
comment: NITRIL_CHT_1
signature sequence match: PROSITE PS00920 match 1

SIPF704FEA34C90239E
type: Structured Name
full name: Nitrilase 2
ec name: 3.5.5.1

SIP37B52E5BEE552DBE
type: Structured Name
full name: Nitrilase II

http://purl.uniprot.org/uniprot/Q6RWK4#gene-MD533CDB4C98051F2033FBB03AE7673E6CC
type: Gene
orf name: BD5070

SIP4A9AE8CF626FEE40
type: Function Annotation
comment: Nitrilases catalyze the mild hydrolytic conversion of organonitriles directly to the corresponding carboxylic acids. Catalyzes the production of aryllactic acid derivatives. Mediates the hydrolysis of cyanohydrin to (S)-phenyllactic acid.

SIP926AEAEAFF11C449
type: Similarity Annotation
comment: Belongs to the carbon-nitrogen hydrolase superfamily. Nitrilase family.

PRO_0000422218
type: Chain Annotation
comment: Nitrilase 2
mass: 34283
range: 22859200393327662tt1tt314

22859200393327662tt1
type: Position
type: ExactPosition
position: 1
reference: Q6RWK4-1

22859200393327662tt314
type: Position
type: ExactPosition
position: 314
reference: Q6RWK4-1

SIP54CAA9552DD4CB5B
type: Domain Extent Annotation
comment: CN hydrolase
range: 22859200393327662tt7tt269

22859200393327662tt7
type: Position
type: ExactPosition
position: 7
reference: Q6RWK4-1

22859200393327662tt269
type: Position
type: ExactPosition
position: 269
reference: Q6RWK4-1

SIP2FE167B904585D72
type: Active Site Annotation
comment: Proton acceptor
range: 22859200393327662tt47tt47

22859200393327662tt47
type: Position
type: ExactPosition
position: 47
reference: Q6RWK4-1

SIP019526BCEF7A03CC
type: Active Site Annotation
comment: Proton donor
range: 22859200393327662tt132tt132

22859200393327662tt132
type: Position
type: ExactPosition
position: 132
reference: Q6RWK4-1

SIP21E66FBF39DA6822
type: Active Site Annotation
comment: Nucleophile
range: 22859200393327662tt166tt166

22859200393327662tt166
type: Position
type: ExactPosition
position: 166
reference: Q6RWK4-1

Q6RWK4-1
type: Simple Sequence
modified: 2004-07-05
version: 1
mass: 34283
value: MGEFGEVTLGVAQAAPVYFDREASTEKARGLIREAGEKGVDLLAFGETWLTGYPYWKDAPWSREYNDLRARYVANGVMIPGPETDALCQAAAEAGVDVAIGVVELEPGSLSSVYCTLLFISREGEILGRHRKLKPTDSERRYWSEGDATGLRVYERPYGRLSGLNCWEHLMMLPGYALAAQGTQFHVAAWPNMASSASELLSRAYAYQAGCYVLCAGGLGPAPGELPDGIAAESLDHLTGESCIIDPWGKVIAGPVSCEETLITARVSTASIYRRKSLTDVGGHYSRPDVFRFEVDRSERPRVVFRDGDVDDRG

http://purl.uniprot.org/uniprot/Q6RWK4#attribution-FA1B07BF743C7ACC7216523C78FA15F9
manual: true
evidence: ECO_0000255
source: PRU00054

http://purl.uniprot.org/uniprot/Q6RWK4#attribution-F91C7F0AE626366E0282B9775FD023B0
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/12148986

http://purl.uniprot.org/uniprot/Q6RWK4#attribution-080ED3982FEC43F7C43A0689717F3D20
manual: true
evidence: ECO_0000305

Q6RWG0
type: Protein
reviewed: true
created: 2013-05-01
modified: 2023-02-22
version: 42
mnemonic: NITR3_UNKP
citation: http://purl.uniprot.org/citations/15066841
citation: http://purl.uniprot.org/citations/12148986
recommended name: SIP7C1946B2395BC9DC
alternative name: SIPBEFFDACC398E6748
enzyme: http://purl.uniprot.org/enzyme/3.5.5.1
organism: http://purl.uniprot.org/taxonomy/2725
annotation: SIPE6D2A5FED8BAA3CA
annotation: SIPF29025ADE79EA7B3
annotation: SIP7522CEADAB9BA0E3
annotation: PRO_0000422219
annotation: SIP7E5BC931917508FF
annotation: SIP14FC303763A9D296
annotation: SIPF8D37AB7DA971A3E
annotation: SIPC861C9028B6BDFE7
annotation: SIPF92BB1FB7A0ED6BB
existence: Inferred from Homology Existence
classified with: http://purl.uniprot.org/keywords/378
classified with: GO_0080061
classified with: GO_0006807
sequence: Q6RWG0-1

Q6RWG0-citation-15066841
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

Q6RWG0-citation-12148986
type: Citation Statement
scope: FUNCTION

SIP7C1946B2395BC9DC
type: Structured Name
full name: Nitrilase 3
ec name: 3.5.5.1

SIPBEFFDACC398E6748
type: Structured Name
full name: Nitrilase III

http://purl.uniprot.org/uniprot/Q6RWG0#gene-MD5A4ABEC0F7A46C5531945AD609D61492C
type: Gene
orf name: BD5308

SIPE6D2A5FED8BAA3CA
type: Function Annotation
comment: Nitrilases catalyze the mild hydrolytic conversion of organonitriles directly to the corresponding carboxylic acids.

SIP7522CEADAB9BA0E3
type: Similarity Annotation
comment: Belongs to the carbon-nitrogen hydrolase superfamily. Nitrilase family.

PRO_0000422219
type: Chain Annotation
comment: Nitrilase 3
mass: 35680
range: 22859200393064494tt1tt330

22859200393064494tt1
type: Position
type: ExactPosition
position: 1
reference: Q6RWG0-1

22859200393064494tt330
type: Position
type: ExactPosition
position: 330
reference: Q6RWG0-1

SIP7E5BC931917508FF
type: Domain Extent Annotation
comment: CN hydrolase
range: 22859200393064494tt4tt273

22859200393064494tt4
type: Position
type: ExactPosition
position: 4
reference: Q6RWG0-1

22859200393064494tt273
type: Position
type: ExactPosition
position: 273
reference: Q6RWG0-1

SIP14FC303763A9D296
type: Region Annotation
comment: Disordered
range: 22859200393064494tt310tt330

22859200393064494tt310
type: Position
type: ExactPosition
position: 310
reference: Q6RWG0-1

SIPF8D37AB7DA971A3E
type: Active Site Annotation
comment: Proton acceptor
range: 22859200393064494tt44tt44

22859200393064494tt44
type: Position
type: ExactPosition
position: 44
reference: Q6RWG0-1

SIPC861C9028B6BDFE7
type: Active Site Annotation
comment: Proton donor
range: 22859200393064494tt128tt128

22859200393064494tt128
type: Position
type: ExactPosition
position: 128
reference: Q6RWG0-1

SIPF92BB1FB7A0ED6BB
type: Active Site Annotation
comment: Nucleophile
range: 22859200393064494tt162tt162

22859200393064494tt162
type: Position
type: ExactPosition
position: 162
reference: Q6RWG0-1

Q6RWG0-1
type: Simple Sequence
modified: 2004-07-05
version: 1
mass: 35680
value: MKVVKAAAVQISPVLYSREATVEKVVKKIHELGQLGVQFATFPETVVPYYPYFSAVQTGIELLSGTEHLRLLDQAVTVPSPATDAIGEAARKAGMVVSIGVNERDGGTLYNTQLLFDADGTLIQRRRKITPTHFERMIWGQGDGSGLRAVDSKVGRIGQLACFEHNNPLARYALIADGEQIHSAMYPGSAFGEGFAQRMEINIRQHALESGAFVVNATAWLDADQQAQIIKDTGCGIGPISGGCFTTIVAPDGMLMAEPLRSGEGEVIVDLDFTLIDRRKMLMDSAGHYNRPELLSLMIDRTATAHVHERAAHPVSGAEQGPEDLRTPAA

http://purl.uniprot.org/uniprot/Q6RWG0#attribution-FA1B07BF743C7ACC7216523C78FA15F9
manual: true
evidence: ECO_0000255
source: PRU00054

http://purl.uniprot.org/uniprot/Q6RWG0#attribution-F91C7F0AE626366E0282B9775FD023B0
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/12148986

http://purl.uniprot.org/uniprot/Q6RWG0#attribution-080ED3982FEC43F7C43A0689717F3D20
manual: true
evidence: ECO_0000305

C1FW05
type: Protein
reviewed: true
created: 2016-11-02
modified: 2023-09-13
version: 43
mnemonic: ORD_UNKP
citation: SIPB768351CF5D342BA
citation: http://purl.uniprot.org/citations/19251850
recommended name: SIPA09CB2845A61D1FF
enzyme: http://purl.uniprot.org/enzyme/1.4.1.26
organism: http://purl.uniprot.org/taxonomy/2725
annotation: SIP24B6205582D068BE
annotation: SIP640FC4A44AA58FB9
annotation: SIP1A8F9AA6D1BC7EE5
annotation: SIPB8FC630A98021F0C
annotation: SIP1274C5A3872CAE75
annotation: SIP3F9CB77DF62FA1AF
annotation: PRO_0000438115
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/21
classified with: http://purl.uniprot.org/keywords/520
classified with: http://purl.uniprot.org/keywords/521
classified with: http://purl.uniprot.org/keywords/560
classified with: GO_0047530
classified with: GO_0008839
classified with: GO_0009089
classified with: GO_0006591
sequence: C1FW05-1

SIPB768351CF5D342BA
type: Submission Citation
group: Genoscope - CEA
date: 2008-04
submitted to: EMBL/GenBank/DDBJ

C1FW05-citation-SIPB768351CF5D342BA
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

C1FW05-citation-19251850
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
scope: FUNCTION
scope: CATALYTIC ACTIVITY
scope: BIOPHYSICOCHEMICAL PROPERTIES
scope: ACTIVITY REGULATION
scope: SUBSTRATE SPECIFICITY
scope: SUBUNIT

IPR045760
type: Resource
database: InterPro
comment: DAP_DH_C

IPR000846
type: Resource
database: InterPro
comment: DapB_N

NF040740
type: Resource
database: NCBIfam
comment: ornith_Ord
signature sequence match: NCBIfam NF040740 match 1

PF19328
type: Resource
database: Pfam
comment: DAP_DH_C
signature sequence match: Pfam PF19328 match 1

PF01113
type: Resource
database: Pfam
comment: DapB_N
signature sequence match: Pfam PF01113 match 1

SIPA09CB2845A61D1FF
type: Structured Name
full name: 2,4-diaminopentanoate dehydrogenase
short name: DAPDH
ec name: 1.4.1.26

ord
type: Gene
orf name: DIG-28672_33

SIP24B6205582D068BE
type: Function Annotation
comment: Involved in the ornithine fermentation pathway. Catalyzes the oxidative deamination of (2R,4S)-2,4-diaminopentanoate ((2R,4S)-DAP) to yield 2-amino-4-ketopentanoate (AKP). Although Ord is more efficient with (2R,4S)-DAP, the diastereoisomer (2R,4R)-DAP can also be metabolized. Ord is specific for NAD as hydrogen acceptor.

SIP1A8F9AA6D1BC7EE5
type: Activity Regulation Annotation
comment: Allosterically activated by (2R,4S)-DAP. Negatively regulated by AKP, and by both acetyl-CoA and D-alanine.

SIPB8FC630A98021F0C
type: Kinetics Annotation
comment: kcat is 34 sec(-1) with NAD as hydrogen acceptor. kcat is 5 sec(-1) with NADP as hydrogen acceptor.
measured affinity: 16 uM for NAD
measured affinity: 2560 uM for NADP

SIP1274C5A3872CAE75
type: Subunit Annotation
comment: Homodimer.

SIP3F9CB77DF62FA1AF
type: Similarity Annotation
comment: Belongs to the DapB family.

PRO_0000438115
type: Chain Annotation
comment: 2,4-diaminopentanoate dehydrogenase
mass: 36466
range: 18913001619862830tt1tt339

18913001619862830tt1
type: Position
type: ExactPosition
position: 1
reference: C1FW05-1

18913001619862830tt339
type: Position
type: ExactPosition
position: 339
reference: C1FW05-1

C1FW05-1
type: Simple Sequence
modified: 2009-05-26
version: 1
mass: 36466
value: MGKNVKTLIWGFGAMGSGMASILLEKGGYELVSVIDTHPQKAGKDVGELLGRAPLGVKVTMDHLKAFGSHPDVALIATSSFVEEVSPQIEFALENDANVITIAEEMSYPWIDSKEIAERLDALALNRGKTVLGTGINPGFVLDTLVVSLSGVCKEVRHIHAKRVNDLAPFGPTVMRTQGVGTTPEKFEEGIRSGNIVGHIGFRQSIMLIAKALGWTIEDIVEERQPIITNVRRKTNYVDVTPGNVAGCRHTARAYSCGREVIFLEHPQQVCPEAEGVRTGDYVVIDGDPPVNLRIEPEIPGGTGTMAMAVNMIPLVVNAPPGLLTMIDLPVPRSISNGF

http://purl.uniprot.org/uniprot/C1FW05#attribution-128961E6BFAD19086473A57836BCA489
creator: UniProtKB
manual: true
evidence: ECO_0000314
source: http://purl.uniprot.org/citations/19251850

http://purl.uniprot.org/uniprot/C1FW05#attribution-F163855B2EA22FED56188F604DB5772A
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/19251850

http://purl.uniprot.org/uniprot/C1FW05#attribution-BE9F2562E1D3B125A6FCB0BE7BC43D41
manual: true
evidence: ECO_0000303
source: http://purl.uniprot.org/citations/19251850

http://purl.uniprot.org/uniprot/C1FW05#attribution-7BD8113461D5AB8AC2988A6FD111FB11
manual: true
evidence: ECO_0000305

http://purl.uniprot.org/uniprot/C1FW05#attribution-E930CB4AD3D2213E7ACCD3A02D6F4367
manual: true
evidence: ECO_0000312
source: CAQ42978.1

C1FW06
type: Protein
reviewed: true
created: 2016-11-02
modified: 2023-09-13
version: 21
mnemonic: ORTA_UNKP
citation: SIPB768351CF5D342BA
citation: http://purl.uniprot.org/citations/19251850
recommended name: SIP52154FA711D10AE2
alternative name: SIPBB1888811A886BE2
enzyme: http://purl.uniprot.org/enzyme/2.3.1.263
organism: http://purl.uniprot.org/taxonomy/2725
annotation: SIP1B74019021071864
annotation: SIP86EBEFFF2546C775
annotation: SIP7C33D850B573FAAB
annotation: SIP8D55E6E2049A2033
annotation: SIP78FFED5CA5EFFC38
annotation: PRO_0000438117
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/808
classified with: GO_0016740
classified with: GO_0006591
sequence: MSEKCKKDDWVEIHYVVLEARERTGDIPEDTRKVPLECWIKGWAEKEGTVGEEVTIRTPANRYVKGTLTRINPEYTHTFGPCASELSAIGQELRATLKEGK

C1FW06-citation-SIPB768351CF5D342BA
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

C1FW06-citation-19251850
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
scope: FUNCTION
scope: CATALYTIC ACTIVITY
scope: BIOPHYSICOCHEMICAL PROPERTIES
scope: SUBSTRATE SPECIFICITY
scope: SUBUNIT

http://purl.uniprot.org/brenda/2.3.1.263
type: Resource
database: BRENDA
comment: 1522

IPR047755
type: Resource
database: InterPro
comment: OrtA

NF040739
type: Resource
database: NCBIfam
comment: ornith_OrtA
signature sequence match: NCBIfam NF040739 match 1

SIP52154FA711D10AE2
type: Structured Name
full name: 2-amino-4-ketopentanoate thiolase alpha subunit
ec name: 2.3.1.263

SIPBB1888811A886BE2
type: Structured Name
full name: AKP thiolase
short name: AKPT

ortA
type: Gene
orf name: DIG-28672_34

SIP1B74019021071864
type: Function Annotation
comment: Involved in the ornithine fermentation pathway. Catalyzes the thiolytic cleavage of 2-amino-4-ketopentanoate (AKP) with coenzyme A (CoA) to form acetyl-CoA and alanine. It is strictly specific for AKP.

SIP7C33D850B573FAAB
type: Kinetics Annotation
comment: kcat is 3.5 sec(-1) for CoA as substrate. kcat is 3.4 sec(-1) for AKP as substrate.
measured affinity: 18 uM for CoA
measured affinity: 35 uM for AKP

SIP8D55E6E2049A2033
type: Subunit Annotation
comment: Heterodimer with OrtB.

SIP78FFED5CA5EFFC38
type: Similarity Annotation
comment: Belongs to the OrtA family.

PRO_0000438117
type: Chain Annotation
comment: 2-amino-4-ketopentanoate thiolase alpha subunit
mass: 11492
range: 18913001619863086tt1tt101

18913001619863086tt1
type: Position
type: ExactPosition
position: 1
reference: MSEKCKKDDWVEIHYVVLEARERTGDIPEDTRKVPLECWIKGWAEKEGTVGEEVTIRTPANRYVKGTLTRINPEYTHTFGPCASELSAIGQELRATLKEGK

18913001619863086tt101
type: Position
type: ExactPosition
position: 101
reference: MSEKCKKDDWVEIHYVVLEARERTGDIPEDTRKVPLECWIKGWAEKEGTVGEEVTIRTPANRYVKGTLTRINPEYTHTFGPCASELSAIGQELRATLKEGK

MSEKCKKDDWVEIHYVVLEARERTGDIPEDTRKVPLECWIKGWAEKEGTVGEEVTIRTPANRYVKGTLTRINPEYTHTFGPCASELSAIGQELRATLKEGK
type: Simple Sequence
modified: 2009-05-26
version: 1
mass: 11492

http://purl.uniprot.org/uniprot/C1FW06#attribution-8D075DC4AC6AEC46D368E4EB1AC5C2A8
creator: UniProtKB
manual: true
evidence: ECO_0000304
source: http://purl.uniprot.org/citations/19251850

http://purl.uniprot.org/uniprot/C1FW06#attribution-F163855B2EA22FED56188F604DB5772A
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/19251850

http://purl.uniprot.org/uniprot/C1FW06#attribution-BE9F2562E1D3B125A6FCB0BE7BC43D41
manual: true
evidence: ECO_0000303
source: http://purl.uniprot.org/citations/19251850

http://purl.uniprot.org/uniprot/C1FW06#attribution-7BD8113461D5AB8AC2988A6FD111FB11
manual: true
evidence: ECO_0000305

http://purl.uniprot.org/uniprot/C1FW06#attribution-383A5FD5ECB23F10AC2A2C229BE49AE4
manual: true
evidence: ECO_0000305
source: http://purl.uniprot.org/citations/19251850

http://purl.uniprot.org/uniprot/C1FW06#attribution-52D8B6794DD2B2605C6E7DD30E35D7B9
manual: true
evidence: ECO_0000312
source: CAQ42979.1

C1FW07
type: Protein
reviewed: true
created: 2016-11-02
modified: 2023-09-13
version: 35
mnemonic: ORTB_UNKP
citation: SIPB768351CF5D342BA
citation: http://purl.uniprot.org/citations/19251850
recommended name: SIP2810959A7AC12E27
alternative name: SIPBB1888811A886BE2
enzyme: http://purl.uniprot.org/enzyme/2.3.1.263
organism: http://purl.uniprot.org/taxonomy/2725
annotation: SIP16AEA7548E0826AC
annotation: SIPCA94C7BB74DB9C9E
annotation: SIP8F1EE021EBB6E5E4
annotation: SIP78810BE32062F039
annotation: SIP0837DCEF8DF56EDF
annotation: SIP339D7B207952A1E9
annotation: PRO_0000438120
annotation: SIPF4C62D9B9F18296B
annotation: SIPC870517336F42E40
annotation: SIP2939CF7252B9FA0B
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/663
classified with: http://purl.uniprot.org/keywords/808
classified with: GO_0003985
classified with: GO_0006591
sequence: C1FW07-1

C1FW07-citation-SIPB768351CF5D342BA
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

C1FW07-citation-19251850
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
scope: FUNCTION
scope: CATALYTIC ACTIVITY
scope: BIOPHYSICOCHEMICAL PROPERTIES
scope: COFACTOR
scope: SUBSTRATE SPECIFICITY
scope: SUBUNIT

cd00640
type: Resource
database: CDD
comment: Trp-synth-beta_II
signature sequence match: CDD cd00640 match 1

IPR001926
type: Resource
database: InterPro
comment: TrpB-like_PALP

IPR036052
type: Resource
database: InterPro
comment: TrpB-like_PALP_sf

NF040741
type: Resource
database: NCBIfam
comment: ornith_OrtB
signature sequence match: NCBIfam NF040741 match 1

PTHR10314
type: Resource
database: PANTHER
comment: CYSTATHIONINE BETA-SYNTHASE
signature sequence match: PANTHER PTHR10314 match 1

PTHR10314:SF5
type: Resource
database: PANTHER
comment: THREONINE SYNTHASE 1, CHLOROPLASTIC
signature sequence match: PANTHER PTHR10314:SF5 match 1

PF00291
type: Resource
database: Pfam
comment: PALP
signature sequence match: Pfam PF00291 match 1

SSF53686
type: Resource
database: SUPFAM
comment: Tryptophan synthase beta subunit-like PLP-dependent enzymes
signature sequence match: SUPFAM SSF53686 match 1

SIP2810959A7AC12E27
type: Structured Name
full name: 2-amino-4-ketopentanoate thiolase beta subunit
ec name: 2.3.1.263

SIPBB1888811A886BE2
type: Structured Name
full name: AKP thiolase
short name: AKPT

ortB
type: Gene
orf name: DIG-28672_35

SIP16AEA7548E0826AC
type: Function Annotation
comment: Involved in the ornithine fermentation pathway. Catalyzes the thiolytic cleavage of 2-amino-4-ketopentanoate (AKP) with coenzyme A (CoA) to form acetyl-CoA and alanine. It is strictly specific for AKP.

SIP78810BE32062F039
type: Kinetics Annotation
comment: kcat is 3.5 sec(-1) for CoA as substrate. kcat is 3.4 sec(-1) for AKP as substrate.
measured affinity: 18 uM for CoA
measured affinity: 35 uM for AKP

SIP0837DCEF8DF56EDF
type: Subunit Annotation
comment: Heterodimer with OrtA.

SIP339D7B207952A1E9
type: Similarity Annotation
comment: Belongs to the threonine synthase family.

PRO_0000438120
type: Chain Annotation
comment: 2-amino-4-ketopentanoate thiolase beta subunit
mass: 51526
range: 18913001619863342tt1tt472

18913001619863342tt1
type: Position
type: ExactPosition
position: 1
reference: C1FW07-1

18913001619863342tt472
type: Position
type: ExactPosition
position: 472
reference: C1FW07-1

18913001619863342tt128
type: Position
type: ExactPosition
position: 128
reference: C1FW07-1

18913001619863342tt238
type: Position
type: ExactPosition
position: 238
reference: C1FW07-1

18913001619863342tt242
type: Position
type: ExactPosition
position: 242
reference: C1FW07-1

SIP2939CF7252B9FA0B
type: Modified Residue Annotation
comment: N6-(pyridoxal phosphate)lysine
range: 18913001619863342tt102tt102

18913001619863342tt102
type: Position
type: ExactPosition
position: 102
reference: C1FW07-1

C1FW07-1
type: Simple Sequence
modified: 2009-05-26
version: 1
mass: 51526
value: MAKDTSYDAVMDRRAEIMSRALGLNYDEFIISDIAFDYEGMMAKAGYSLEEVRQIQSESGVGNTPLLELRNITDLARKTSKRGFGARILIKDEAANPSGSFKDRRASVSIHHAKKLGYPGVLAATSGNYGAAVASQAAMKNLGCIIVQEVFDSRHVGQPEIIEKSRKCEAYGAEVVQLTVGPELFYVSLILLEETGYFNASLYSPFGISGVETLGYELVEQIRARYDKDPAYIVVTHAGGGNVTGTARGALKAGAKNSTIIGASVDLSGLHMASDNDFNKKSFTTGHTGFGVPFATWPDRTDVPKNAARPLRYLDRYVTVTQGEVFYVTEALAQVEGMERGPAGNTSLAAAIALARELPEDEIVVVQETEYTGAGKHPWAQLDFAKQNGIAVKRGAPKENKPGKTIVIPQNFSQITATEMDLNRMRRSYIRNALERNKVKNVTEEDIRFLAEDTKTDADFVTSVIRDLGVRL

http://purl.uniprot.org/uniprot/C1FW07#attribution-D494480899ADFC6EA181045CAB863938
creator: UniProtKB
manual: true
evidence: ECO_0000303
source: http://purl.uniprot.org/citations/19251850

http://purl.uniprot.org/uniprot/C1FW07#attribution-8D075DC4AC6AEC46D368E4EB1AC5C2A8
creator: UniProtKB
manual: true
evidence: ECO_0000304
source: http://purl.uniprot.org/citations/19251850

http://purl.uniprot.org/uniprot/C1FW07#attribution-09832D771DD146614C549A190F6BD2AF
manual: true
evidence: ECO_0000250
source: P9WG59

http://purl.uniprot.org/uniprot/C1FW07#attribution-F163855B2EA22FED56188F604DB5772A
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/19251850

http://purl.uniprot.org/uniprot/C1FW07#attribution-BE9F2562E1D3B125A6FCB0BE7BC43D41
manual: true
evidence: ECO_0000303
source: http://purl.uniprot.org/citations/19251850

http://purl.uniprot.org/uniprot/C1FW07#attribution-080ED3982FEC43F7C43A0689717F3D20
manual: true
evidence: ECO_0000305

http://purl.uniprot.org/uniprot/C1FW07#attribution-383A5FD5ECB23F10AC2A2C229BE49AE4
manual: true
evidence: ECO_0000305
source: http://purl.uniprot.org/citations/19251850

http://purl.uniprot.org/uniprot/C1FW07#attribution-40B6FA724358C288601908139E8CC42A
manual: true
evidence: ECO_0000312
source: CAQ42980.1

G9BY57
type: Protein
reviewed: true
created: 2020-08-12
modified: 2023-09-13
version: 38
mnemonic: PETH_UNKP
citation: http://purl.uniprot.org/citations/22194294
citation: http://purl.uniprot.org/citations/24593046
citation: http://purl.uniprot.org/citations/32269349
recommended name: SIP572E78689E67FC51
alternative name: SIP47D9A88D2DA5E4BB
alternative name: SIPF135F656C04DA087
enzyme: http://purl.uniprot.org/enzyme/3.1.1.74
enzyme: http://purl.uniprot.org/enzyme/3.1.1.101
organism: http://purl.uniprot.org/taxonomy/2725
annotation: SIP8767786D2E1FBEE3
annotation: SIP5344D9ABCFF31775
annotation: SIP16E6703B008E0039
annotation: SIP07F1044156226508
annotation: SIP242ED945350B82E1
annotation: SIPACAB64918B89F406
annotation: SIP92FA80026C2874D8
annotation: SIP950B82125A93E0CF
annotation: SIPA17D92B5AAE84D43
annotation: SIP2D6BFA90B46D5546
annotation: SIP0BDE4C0C2F252794
annotation: SIP43397ACB04E88200
annotation: SIP4B408D5DF023D945
annotation: SIP409544ABF2429C94
annotation: SIPB933C06775425C7B
annotation: SIP814AD8ABB34E33FE
annotation: SIP19922BC2631997E6
annotation: SIPA631CAED053DABAC
annotation: PRO_5003520086
annotation: SIP05713D927F87A5C5
annotation: SIPB03B577030D9F4AB
annotation: SIPF4E4E24616A6BA86
annotation: SIP6E01D55D30C88B60
annotation: SIPB48245FF4CD7E323
annotation: SIPC34D7A38B843D2D6
annotation: SIPFDF0F021394EDC0F
annotation: SIP5689C4EDCB20FCA7
annotation: SIP0A614554C3E0898B
annotation: SIP6F23ABBE07FD26D8
annotation: SIPEEF584A223E1B7B5
annotation: SIP226CC6C46C30386A
annotation: SIPC155C0F55C3036C6
annotation: SIP912AABEC534D3491
annotation: SIP54CE6C0EB206A7AC
annotation: SIP671229756719E262
annotation: SIP553F3FCD8ACB1643
annotation: SIP6FB8BCEBE29AF3A5
annotation: SIP4378A71EF3DE21E2
annotation: SIP26704D7D86C8DE4B
annotation: SIP157209915C8E5F26
annotation: SIPB13929C3DF5E3E95
annotation: SIP4D0DBA19FCECF719
annotation: SIP7BACCB72D46F7C20
annotation: SIP96CCC966C15C97F4
annotation: SIP0455CDB18069D5F1
annotation: SIPB36298F23D68E428
annotation: SIPBA74F15C90E57082
annotation: SIP696E5C896401E2B8
annotation: SIPA018094CEBE10C9B
annotation: SIPC252ACD8AA32EF6C
annotation: SIPA0732DB1D01B407A
annotation: SIP752E74B729CA0986
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/2
classified with: http://purl.uniprot.org/keywords/1015
classified with: http://purl.uniprot.org/keywords/964
classified with: http://purl.uniprot.org/keywords/719
classified with: http://purl.uniprot.org/keywords/732
classified with: GO_0005576
classified with: GO_0008126
classified with: GO_0050525
sequence: G9BY57-1

G9BY57-citation-22194294
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
scope: FUNCTION
scope: CATALYTIC ACTIVITY
scope: SUBSTRATE SPECIFICITY
scope: BIOPHYSICOCHEMICAL PROPERTIES
scope: SUBCELLULAR LOCATION
scope: ACTIVITY REGULATION
scope: MUTAGENESIS OF SER-165

G9BY57-citation-24593046
type: Citation Statement
scope: X-RAY CRYSTALLOGRAPHY (1.50 ANGSTROMS) OF 36-293
scope: FUNCTION
scope: CATALYTIC ACTIVITY
scope: BIOPHYSICOCHEMICAL PROPERTIES
scope: DISULFIDE BOND
scope: ACTIVE SITE

G9BY57-citation-32269349
type: Citation Statement
scope: X-RAY CRYSTALLOGRAPHY (1.10 ANGSTROMS) OF 36-293 OF MUTANTS ALA-165 AND ILE-243/CYS-238/CYS-283/GLY-127
scope: FUNCTION
scope: CATALYTIC ACTIVITY
scope: BIOTECHNOLOGY
scope: PROTEIN ENGINEERING
scope: MUTAGENESIS STUDY
scope: MUTAGENESIS OF PHE-243
scope: BIOPHYSICOCHEMICAL PROPERTIES
scope: DISULFIDE BOND
scope: ACTIVE SITE

4EB0
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 1.50
chain sequence mapping: PDB 4EB0 tt36tt293

PDB 4EB0 tt36tt293
chain: A=36-293

6THS
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 1.10
chain sequence mapping: PDB 6THS tt36tt293

PDB 6THS tt36tt293
chain: A=36-293

6THT
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 1.14
chain sequence mapping: PDB 6THT tt36tt293

PDB 6THT tt36tt293
chain: A=36-293

7VVC
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 1.82
chain sequence mapping: PDB 7VVC tt36tt293

PDB 7VVC tt36tt293
chain: A/B=36-293

7VVE
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 1.98
chain sequence mapping: PDB 7VVE tt36tt293

PDB 7VVE tt36tt293
chain: A/B=36-293

7W1N
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 1.88
chain sequence mapping: PDB 7W1N tt36tt293

PDB 7W1N tt36tt293
chain: A=36-293

7W44
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 1.85
chain sequence mapping: PDB 7W44 tt35tt293

PDB 7W44 tt35tt293
chain: A/B=35-293

7W45
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 1.94
chain sequence mapping: PDB 7W45 tt36tt293

PDB 7W45 tt36tt293
chain: A/B=36-293

9bact-g9by57
type: Resource
database: ESTHER
comment: Polyesterase-lipase-cutinase

IPR029059
type: Resource
database: InterPro
comment: AB_hydrolase_5

PTHR22946:SF9
type: Resource
database: PANTHER
comment: POLYKETIDE TRANSFERASE AF380
signature sequence match: PANTHER PTHR22946:SF9 match 1

PTHR22946
type: Resource
database: PANTHER
comment: UNCHARACTERIZED
signature sequence match: PANTHER PTHR22946 match 1

PF12695
type: Resource
database: Pfam
comment: Abhydrolase_5
signature sequence match: Pfam PF12695 match 1

SIP572E78689E67FC51
type: Structured Name
full name: Leaf-branch compost cutinase
short name: LC-cutinase
short name: LCC
ec name: 3.1.1.74

SIP47D9A88D2DA5E4BB
type: Structured Name
full name: PET-digesting enzyme

SIPF135F656C04DA087
type: Structured Name
full name: Poly(ethylene terephthalate) hydrolase
short name: PET hydrolase
short name: PETase
ec name: 3.1.1.101

SIP8767786D2E1FBEE3
type: Function Annotation
comment: Catalyzes the hydrolysis of cutin, a polyester that forms the structure of plant cuticle (PubMed:22194294). Shows esterase activity towards p-nitrophenol-linked aliphatic esters (pNP-aliphatic esters), with a preference for short-chain substrates (C4 substrate at most) (PubMed:22194294, PubMed:24593046). Cannot hydrolyze olive oil (PubMed:22194294). Is also able to degrade poly(ethylene terephthalate), the most abundant polyester plastic in the world (PubMed:22194294, PubMed:32269349). Can also depolymerize poly(epsilon-caprolactone) (PCL), a synthetic aliphatic biodegradable polyester (PubMed:22194294).

SIPA17D92B5AAE84D43
type: Activity Regulation Annotation
comment: Is inhibited in vitro by diethyl pNP-phosphate (E600), but not by EDTA.

SIP2D6BFA90B46D5546
type: Kinetics Annotation
comment: kcat is 232 sec(-1) with pNP-butanoate as substrate (at 30 degrees Celsius and pH 8.0). kcat is 343 sec(-1) with pNP-butanoate as substrate (at 50 degrees Celsius and pH 8.0). kcat is 318 sec(-1) with pNP-butanoate as substrate (at 70 degrees Celsius and pH 8.0).
measured affinity: 0.22 mM for pNP-butanoate (at 30 degrees Celsius and pH 8.0)
measured affinity: 0.21 mM for pNP-butanoate (at 50 degrees Celsius and pH 8.0)
measured affinity: 0.24 mM for pNP-butanoate (at 70 degrees Celsius and pH 8.0)

SIP0BDE4C0C2F252794
type: PH Dependence Annotation
comment: Optimum pH is 8.5 with pNP-butyrate as substrate. Shows 70% of the maximal activity at pH 7.0 and pH 9.5.

SIP43397ACB04E88200
type: Temperature Dependence Annotation
comment: Optimum temperature is 50 degrees Celsius with pNP-butanoate as substrate (PubMed:22194294, PubMed:24593046). Optimum temperature using PET as substrate is superior to 70 degrees Celsius (PubMed:22194294). Shows 70% of the maximal activity at 30 and 70 degrees Celsius with pNP-butyrate as substrate. Has half-lives of 5 hours at 50 degrees Celsius, 80 minutes at 60 degrees Celsius, 40 minutes at 70 degrees Celsius and 7 minutes at 80 degrees Celsius (PubMed:22194294). Is thermostable, with a determined melting temperature (Tm) of 84.7 degrees Celsius (PubMed:32269349).

SIP409544ABF2429C94
type: PTM Annotation
comment: The disulfide bond between Cys-275 and Cys-292 contributes not only to the thermodynamic stability but also to the kinetic stability of the enzyme.

SIPB933C06775425C7B
type: Biotechnology Annotation
comment: The engineered enzyme with mutations Ile-243/Cys-238/Cys-283/Gly-127 can hydrolyze PET in a highly efficient way, ultimately achieving, in less than 10 hours, a minimum of 90 per cent PET depolymerization into monomers, with a productivity of 16.7 grams of terephthalate per liter per hour. It was also shown that biologically recycled PET produced from enzymatically depolymerized PET waste exhibits the same properties as petrochemical PET, before being processed into bottles, thereby contributing towards the concept of a circular PET economy.

SIP814AD8ABB34E33FE
type: Annotation
comment: The gene encoding this enzyme comes from a leaf-branch compost metagenome. The source organism remains to be identified. However, it is presumably a thermophilic bacterium.

SIP19922BC2631997E6
type: Similarity Annotation
comment: Belongs to the AB hydrolase superfamily.

20047680473741102tt1
type: Position
type: ExactPosition
position: 1
reference: G9BY57-1

20047680473741102tt34
type: Position
type: ExactPosition
position: 34
reference: G9BY57-1

PRO_5003520086
type: Chain Annotation
comment: Leaf-branch compost cutinase
mass: 27902
range: 20047680473741102tt35tt293

20047680473741102tt35
type: Position
type: ExactPosition
position: 35
reference: G9BY57-1

20047680473741102tt293
type: Position
type: ExactPosition
position: 293
reference: G9BY57-1

SIP05713D927F87A5C5
type: Active Site Annotation
comment: Nucleophile
range: 20047680473741102tt165tt165

20047680473741102tt165
type: Position
type: ExactPosition
position: 165
reference: G9BY57-1

SIPB03B577030D9F4AB
type: Active Site Annotation
comment: Charge relay system
range: 20047680473741102tt210tt210

20047680473741102tt210
type: Position
type: ExactPosition
position: 210
reference: G9BY57-1

SIPF4E4E24616A6BA86
type: Active Site Annotation
comment: Charge relay system
range: 20047680473741102tt242tt242

20047680473741102tt242
type: Position
type: ExactPosition
position: 242
reference: G9BY57-1

20047680473741102tt95
type: Position
type: ExactPosition
position: 95
reference: G9BY57-1

20047680473741102tt166
type: Position
type: ExactPosition
position: 166
reference: G9BY57-1

20047680473741102tt190
type: Position
type: ExactPosition
position: 190
reference: G9BY57-1

20047680473741102tt275
type: Position
type: ExactPosition
position: 275
reference: G9BY57-1

20047680473741102tt292
type: Position
type: ExactPosition
position: 292
reference: G9BY57-1

SIP5689C4EDCB20FCA7
type: Mutagenesis Annotation
comment: Loss of catalytic activity.
substitution: A
range: 20047680473741102tt165tt165

SIP0A614554C3E0898B
type: Mutagenesis Annotation
comment: Increased PET-depolymerization activity.
substitution: I
substitution: W
range: 20047680473741102tt243tt243

20047680473741102tt243
type: Position
type: ExactPosition
position: 243
reference: G9BY57-1

20047680473741102tt48
type: Position
type: ExactPosition
position: 48
reference: G9BY57-1

20047680473741102tt51
type: Position
type: ExactPosition
position: 51
reference: G9BY57-1

20047680473741102tt55
type: Position
type: ExactPosition
position: 55
reference: G9BY57-1

20047680473741102tt63
type: Position
type: ExactPosition
position: 63
reference: G9BY57-1

20047680473741102tt65
type: Position
type: ExactPosition
position: 65
reference: G9BY57-1

20047680473741102tt67
type: Position
type: ExactPosition
position: 67
reference: G9BY57-1

20047680473741102tt69
type: Position
type: ExactPosition
position: 69
reference: G9BY57-1

20047680473741102tt71
type: Position
type: ExactPosition
position: 71
reference: G9BY57-1

20047680473741102tt74
type: Position
type: ExactPosition
position: 74
reference: G9BY57-1

20047680473741102tt81
type: Position
type: ExactPosition
position: 81
reference: G9BY57-1

20047680473741102tt86
type: Position
type: ExactPosition
position: 86
reference: G9BY57-1

20047680473741102tt92
type: Position
type: ExactPosition
position: 92
reference: G9BY57-1

20047680473741102tt99
type: Position
type: ExactPosition
position: 99
reference: G9BY57-1

20047680473741102tt102
type: Position
type: ExactPosition
position: 102
reference: G9BY57-1

20047680473741102tt103
type: Position
type: ExactPosition
position: 103
reference: G9BY57-1

20047680473741102tt111
type: Position
type: ExactPosition
position: 111
reference: G9BY57-1

20047680473741102tt115
type: Position
type: ExactPosition
position: 115
reference: G9BY57-1

20047680473741102tt119
type: Position
type: ExactPosition
position: 119
reference: G9BY57-1

20047680473741102tt128
type: Position
type: ExactPosition
position: 128
reference: G9BY57-1

20047680473741102tt144
type: Position
type: ExactPosition
position: 144
reference: G9BY57-1

20047680473741102tt148
type: Position
type: ExactPosition
position: 148
reference: G9BY57-1

20047680473741102tt151
type: Position
type: ExactPosition
position: 151
reference: G9BY57-1

20047680473741102tt154
type: Position
type: ExactPosition
position: 154
reference: G9BY57-1

20047680473741102tt164
type: Position
type: ExactPosition
position: 164
reference: G9BY57-1

20047680473741102tt177
type: Position
type: ExactPosition
position: 177
reference: G9BY57-1

20047680473741102tt184
type: Position
type: ExactPosition
position: 184
reference: G9BY57-1

20047680473741102tt188
type: Position
type: ExactPosition
position: 188
reference: G9BY57-1

20047680473741102tt202
type: Position
type: ExactPosition
position: 202
reference: G9BY57-1

20047680473741102tt207
type: Position
type: ExactPosition
position: 207
reference: G9BY57-1

20047680473741102tt211
type: Position
type: ExactPosition
position: 211
reference: G9BY57-1

20047680473741102tt213
type: Position
type: ExactPosition
position: 213
reference: G9BY57-1

20047680473741102tt215
type: Position
type: ExactPosition
position: 215
reference: G9BY57-1

20047680473741102tt218
type: Position
type: ExactPosition
position: 218
reference: G9BY57-1

20047680473741102tt219
type: Position
type: ExactPosition
position: 219
reference: G9BY57-1

20047680473741102tt225
type: Position
type: ExactPosition
position: 225
reference: G9BY57-1

20047680473741102tt232
type: Position
type: ExactPosition
position: 232
reference: G9BY57-1

20047680473741102tt237
type: Position
type: ExactPosition
position: 237
reference: G9BY57-1

20047680473741102tt244
type: Position
type: ExactPosition
position: 244
reference: G9BY57-1

20047680473741102tt246
type: Position
type: ExactPosition
position: 246
reference: G9BY57-1

20047680473741102tt250
type: Position
type: ExactPosition
position: 250
reference: G9BY57-1

20047680473741102tt264
type: Position
type: ExactPosition
position: 264
reference: G9BY57-1

20047680473741102tt268
type: Position
type: ExactPosition
position: 268
reference: G9BY57-1

20047680473741102tt273
type: Position
type: ExactPosition
position: 273
reference: G9BY57-1

20047680473741102tt282
type: Position
type: ExactPosition
position: 282
reference: G9BY57-1

20047680473741102tt287
type: Position
type: ExactPosition
position: 287
reference: G9BY57-1

20047680473741102tt290
type: Position
type: ExactPosition
position: 290
reference: G9BY57-1

G9BY57-1
type: Simple Sequence
modified: 2012-02-22
version: 1
precursor: true
mass: 31496
value: MDGVLWRVRTAALMAALLALAAWALVWASPSVEAQSNPYQRGPNPTRSALTADGPFSVATYTVSRLSVSGFGGGVIYYPTGTSLTFGGIAMSPGYTADASSLAWLGRRLASHGFVVLVINTNSRFDYPDSRASQLSAALNYLRTSSPSAVRARLDANRLAVAGHSMGGGGTLRIAEQNPSLKAAVPLTPWHTDKTFNTSVPVLIVGAEADTVAPVSQHAIPFYQNLPSTTPKVYVELDNASHFAPNSNNAAISVYTISWMKLWVDNDTRYRQFLCNVNDPALSDFRTNNRHCQ

http://purl.uniprot.org/uniprot/G9BY57#attribution-265B5DEF50FE8F8D5274B6C8A191BC69
manual: true
evidence: ECO_0000250
source: A0A0K8P6T7

http://purl.uniprot.org/uniprot/G9BY57#attribution-8186E24FBB9D658BDA342CC35AAE333E
manual: true
evidence: ECO_0000255

http://purl.uniprot.org/uniprot/G9BY57#attribution-49AD36EC2AF8EF0632F502549244B1A6
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/22194294

http://purl.uniprot.org/uniprot/G9BY57#attribution-6035D48537D2CBD66F773B19DAFEE192
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/24593046

http://purl.uniprot.org/uniprot/G9BY57#attribution-1D14BDE7C1704C33C25485054AD22E5A
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/32269349

http://purl.uniprot.org/uniprot/G9BY57#attribution-68A06656E1189A6F2EDAEE388CF264B8
manual: true
evidence: ECO_0000303
source: http://purl.uniprot.org/citations/22194294

http://purl.uniprot.org/uniprot/G9BY57#attribution-9052FA2C52C915E98B8305670DF8D77A
manual: true
evidence: ECO_0000303
source: http://purl.uniprot.org/citations/24593046

http://purl.uniprot.org/uniprot/G9BY57#attribution-8E0FECEDE65E18FF1F542F63BEC00C68
manual: true
evidence: ECO_0000305

http://purl.uniprot.org/uniprot/G9BY57#attribution-4FB0B92AE1FDC17774C3C7C57C6282A6
manual: true
evidence: ECO_0000305
source: http://purl.uniprot.org/citations/22194294

http://purl.uniprot.org/uniprot/G9BY57#attribution-F5B967B31A5F48246294339089859C29
manual: true
evidence: ECO_0000305
source: http://purl.uniprot.org/citations/24593046

http://purl.uniprot.org/uniprot/G9BY57#attribution-72E4A136CE7D26946C562D8F08E6FFA8
manual: true
evidence: ECO_0000305
source: http://purl.uniprot.org/citations/32269349

http://purl.uniprot.org/uniprot/G9BY57#attribution-1C0F7CC41E80912449805034B8AA60AC
manual: true
evidence: ECO_0007744
source: 4EB0

http://purl.uniprot.org/uniprot/G9BY57#attribution-B9F00DB277A82BC1661814CF1F72DFE2
manual: true
evidence: ECO_0007744
source: 6THS

http://purl.uniprot.org/uniprot/G9BY57#attribution-28BDA38F23B1A97C03C278B914E7E709
manual: true
evidence: ECO_0007744
source: 6THT

P85173
type: Protein
reviewed: true
created: 2007-07-10
modified: 2023-02-22
version: 48
mnemonic: PHBP_UNKP
old mnemonic: PHBP_HUMAN
citation: http://purl.uniprot.org/citations/18076037
citation: http://purl.uniprot.org/citations/16531243
recommended name: SIP0AA969B3CE7F46B9
organism: http://purl.uniprot.org/taxonomy/2725
isolated from: http://purl.uniprot.org/tissues/800
annotation: SIPC19340FA5E5FAF9F
annotation: SIP1559596C9CEC4F95
annotation: SIP113CD737FBA54AB5
annotation: SIP77C059638A5CD8D9
annotation: SIP16912D422267FF42
annotation: SIP159925FB5D60C494
annotation: SIP408213AE48529211
annotation: PRO_0000295168
annotation: SIP540A6A11EF8B6E64
annotation: SIP56D7EB9B9E1CBC9C
annotation: SIP437227260AA7273F
annotation: SIPFCF8E2A622B3272D
annotation: SIPD1592D056DA8EE4C
annotation: SIP37D1D66F8B5BA64B
annotation: SIP9E11DDA350E9E23C
annotation: SIP7ABEFE55A41A696E
annotation: SIPC0C9B5DCA50E006E
annotation: SIP3F27329C661566BF
annotation: SIPD03F34C5E08F1F11
annotation: SIP2CC0CD940D694756
annotation: SIP232F3A843A01A6EF
annotation: SIP6163FA0227DC22B9
annotation: SIPA59F7EDF1B2B236E
annotation: SIPA4E39A73B8A2E898
annotation: SIPA6426D94EBCC3478
annotation: SIP3F8521739353BD5E
annotation: SIP12D97522EFBC6755
annotation: SIPD28539336C420F5B
annotation: SIPF0DAEC0C0A6CADCA
annotation: SIP611D28259A45270B
annotation: SIPA3051F36B2E30F39
annotation: SIP3717541D4A63F227
annotation: SIP1DCC28EACA26A91D
annotation: SIPC46156DBB0AC91B7
annotation: SIP207E59A0E9E3F3FC
annotation: SIP3D667FA63E370B00
annotation: SIP7E18E47654A05644
annotation: SIPB4BE11026AA00DD6
annotation: SIP14A42FB522267FE3
annotation: SIP7512E592E12A5320
annotation: SIP98692C0205DB1800
annotation: SIPB7F029C07EE7F07B
annotation: SIP74B09E91BC67C9F4
annotation: SIP264FBA5422A5024F
annotation: SIPA92940AA7E52AD0C
annotation: SIP8764F0374358D210
annotation: SIP4016DBA62A77F74B
annotation: SIPB2B73E6B9A640EC0
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/2
classified with: http://purl.uniprot.org/keywords/903
classified with: http://purl.uniprot.org/keywords/1015
classified with: http://purl.uniprot.org/keywords/592
classified with: http://purl.uniprot.org/keywords/964
classified with: GO_0005576
classified with: GO_0006817
sequence: P85173-1

P85173-citation-18076037
type: Citation Statement
scope: PROTEIN SEQUENCE
scope: FUNCTION
scope: MASS SPECTROMETRY

Plasma
type: Tissue

P85173-citation-16531243
type: Citation Statement
scope: PROTEIN SEQUENCE OF 1-33; 38-125; 127-152; 197-230; 246-298; 313-321; 332-347 AND 356-372
scope: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS)
scope: INTERACTION WITH HUMAN PON1
scope: DISULFIDE BONDS
scope: TISSUE SPECIFICITY
scope: SUBCELLULAR LOCATION

2V3Q
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 1.89
chain sequence mapping: PDB 2V3Q tt1tt376

PDB 2V3Q tt1tt376
chain: A=1-376

3W9V
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 1.03
chain sequence mapping: PDB 3W9V tt1tt376

PDB 3W9V tt1tt376
chain: A/B=1-376

3W9W
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 1.35
chain sequence mapping: PDB 3W9W tt1tt376

PDB 3W9W tt1tt376
chain: A/B=1-376

4M1V
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 1.30
chain sequence mapping: PDB 4M1V tt1tt376

PDB 4M1V tt1tt376
chain: A=1-376

http://purl.uniprot.org/gene3d/3.40.190.10
type: Resource
database: Gene3D
comment: Periplasmic binding protein-like II
signature sequence match: Gene3D 3.40.190.10 match 1
signature sequence match: Gene3D 3.40.190.10 match 2

IPR024370
type: Resource
database: InterPro
comment: PBP_domain

PTHR42996
type: Resource
database: PANTHER
comment: PHOSPHATE-BINDING PROTEIN PSTS
signature sequence match: PANTHER PTHR42996 match 1

PTHR42996:SF1
type: Resource
database: PANTHER
comment: PHOSPHATE-BINDING PROTEIN PSTS
signature sequence match: PANTHER PTHR42996:SF1 match 1

PF12849
type: Resource
database: Pfam
comment: PBP_like_2
signature sequence match: Pfam PF12849 match 1

SSF53850
type: Resource
database: SUPFAM
comment: Periplasmic binding protein-like II
signature sequence match: SUPFAM SSF53850 match 1

SIP0AA969B3CE7F46B9
type: Structured Name
full name: Phosphate-binding protein
short name: HPBP

SIPC19340FA5E5FAF9F
type: Function Annotation
comment: Phosphate-binding protein.

SIP1559596C9CEC4F95
type: Subunit Annotation
comment: Heterooligomer with human PON1.

SIP77C059638A5CD8D9
type: Tissue Specificity Annotation
comment: Found in human plasma.

SIP16912D422267FF42
type: Mass Spectrometry Annotation
measured value: 38529.11
measured error: 0.06
method: ESI

SIP159925FB5D60C494
type: Similarity Annotation
comment: Belongs to the PstS family.

SIP408213AE48529211
type: Caution Annotation
comment: Although this is said to be a human protein, it is not encoded in the human genome. It is highly similar to microbial proteins and belongs to a family of microbial proteins.

PRO_0000295168
type: Chain Annotation
comment: Phosphate-binding protein
mass: 38533
range: 22579799246975790tt1tt376

22579799246975790tt1
type: Position
type: ExactPosition
position: 1
reference: P85173-1

22579799246975790tt376
type: Position
type: ExactPosition
position: 376
reference: P85173-1

22579799246975790tt113
type: Position
type: ExactPosition
position: 113
reference: P85173-1

22579799246975790tt158
type: Position
type: ExactPosition
position: 158
reference: P85173-1

22579799246975790tt306
type: Position
type: ExactPosition
position: 306
reference: P85173-1

22579799246975790tt369
type: Position
type: ExactPosition
position: 369
reference: P85173-1

22579799246975790tt4
type: Position
type: ExactPosition
position: 4
reference: P85173-1

22579799246975790tt6
type: Position
type: ExactPosition
position: 6
reference: P85173-1

22579799246975790tt11
type: Position
type: ExactPosition
position: 11
reference: P85173-1

22579799246975790tt14
type: Position
type: ExactPosition
position: 14
reference: P85173-1

22579799246975790tt17
type: Position
type: ExactPosition
position: 17
reference: P85173-1

22579799246975790tt19
type: Position
type: ExactPosition
position: 19
reference: P85173-1

22579799246975790tt32
type: Position
type: ExactPosition
position: 32
reference: P85173-1

22579799246975790tt41
type: Position
type: ExactPosition
position: 41
reference: P85173-1

22579799246975790tt44
type: Position
type: ExactPosition
position: 44
reference: P85173-1

22579799246975790tt46
type: Position
type: ExactPosition
position: 46
reference: P85173-1

22579799246975790tt57
type: Position
type: ExactPosition
position: 57
reference: P85173-1

22579799246975790tt62
type: Position
type: ExactPosition
position: 62
reference: P85173-1

22579799246975790tt66
type: Position
type: ExactPosition
position: 66
reference: P85173-1

22579799246975790tt75
type: Position
type: ExactPosition
position: 75
reference: P85173-1

22579799246975790tt77
type: Position
type: ExactPosition
position: 77
reference: P85173-1

22579799246975790tt80
type: Position
type: ExactPosition
position: 80
reference: P85173-1

22579799246975790tt83
type: Position
type: ExactPosition
position: 83
reference: P85173-1

22579799246975790tt92
type: Position
type: ExactPosition
position: 92
reference: P85173-1

22579799246975790tt109
type: Position
type: ExactPosition
position: 109
reference: P85173-1

22579799246975790tt117
type: Position
type: ExactPosition
position: 117
reference: P85173-1

22579799246975790tt123
type: Position
type: ExactPosition
position: 123
reference: P85173-1

22579799246975790tt125
type: Position
type: ExactPosition
position: 125
reference: P85173-1

22579799246975790tt136
type: Position
type: ExactPosition
position: 136
reference: P85173-1

22579799246975790tt142
type: Position
type: ExactPosition
position: 142
reference: P85173-1

22579799246975790tt145
type: Position
type: ExactPosition
position: 145
reference: P85173-1

22579799246975790tt157
type: Position
type: ExactPosition
position: 157
reference: P85173-1

22579799246975790tt160
type: Position
type: ExactPosition
position: 160
reference: P85173-1

22579799246975790tt163
type: Position
type: ExactPosition
position: 163
reference: P85173-1

22579799246975790tt171
type: Position
type: ExactPosition
position: 171
reference: P85173-1

22579799246975790tt173
type: Position
type: ExactPosition
position: 173
reference: P85173-1

22579799246975790tt182
type: Position
type: ExactPosition
position: 182
reference: P85173-1

22579799246975790tt184
type: Position
type: ExactPosition
position: 184
reference: P85173-1

22579799246975790tt186
type: Position
type: ExactPosition
position: 186
reference: P85173-1

22579799246975790tt189
type: Position
type: ExactPosition
position: 189
reference: P85173-1

22579799246975790tt190
type: Position
type: ExactPosition
position: 190
reference: P85173-1

22579799246975790tt199
type: Position
type: ExactPosition
position: 199
reference: P85173-1

22579799246975790tt213
type: Position
type: ExactPosition
position: 213
reference: P85173-1

22579799246975790tt215
type: Position
type: ExactPosition
position: 215
reference: P85173-1

22579799246975790tt216
type: Position
type: ExactPosition
position: 216
reference: P85173-1

22579799246975790tt219
type: Position
type: ExactPosition
position: 219
reference: P85173-1

22579799246975790tt220
type: Position
type: ExactPosition
position: 220
reference: P85173-1

22579799246975790tt224
type: Position
type: ExactPosition
position: 224
reference: P85173-1

22579799246975790tt226
type: Position
type: ExactPosition
position: 226
reference: P85173-1

22579799246975790tt228
type: Position
type: ExactPosition
position: 228
reference: P85173-1

22579799246975790tt235
type: Position
type: ExactPosition
position: 235
reference: P85173-1

22579799246975790tt237
type: Position
type: ExactPosition
position: 237
reference: P85173-1

22579799246975790tt240
type: Position
type: ExactPosition
position: 240
reference: P85173-1

22579799246975790tt242
type: Position
type: ExactPosition
position: 242
reference: P85173-1

22579799246975790tt249
type: Position
type: ExactPosition
position: 249
reference: P85173-1

22579799246975790tt251
type: Position
type: ExactPosition
position: 251
reference: P85173-1

22579799246975790tt253
type: Position
type: ExactPosition
position: 253
reference: P85173-1

22579799246975790tt257
type: Position
type: ExactPosition
position: 257
reference: P85173-1

22579799246975790tt264
type: Position
type: ExactPosition
position: 264
reference: P85173-1

22579799246975790tt266
type: Position
type: ExactPosition
position: 266
reference: P85173-1

22579799246975790tt270
type: Position
type: ExactPosition
position: 270
reference: P85173-1

22579799246975790tt273
type: Position
type: ExactPosition
position: 273
reference: P85173-1

22579799246975790tt277
type: Position
type: ExactPosition
position: 277
reference: P85173-1

22579799246975790tt280
type: Position
type: ExactPosition
position: 280
reference: P85173-1

22579799246975790tt285
type: Position
type: ExactPosition
position: 285
reference: P85173-1

22579799246975790tt287
type: Position
type: ExactPosition
position: 287
reference: P85173-1

22579799246975790tt290
type: Position
type: ExactPosition
position: 290
reference: P85173-1

22579799246975790tt292
type: Position
type: ExactPosition
position: 292
reference: P85173-1

22579799246975790tt296
type: Position
type: ExactPosition
position: 296
reference: P85173-1

22579799246975790tt305
type: Position
type: ExactPosition
position: 305
reference: P85173-1

22579799246975790tt307
type: Position
type: ExactPosition
position: 307
reference: P85173-1

22579799246975790tt309
type: Position
type: ExactPosition
position: 309
reference: P85173-1

22579799246975790tt310
type: Position
type: ExactPosition
position: 310
reference: P85173-1

22579799246975790tt323
type: Position
type: ExactPosition
position: 323
reference: P85173-1

22579799246975790tt326
type: Position
type: ExactPosition
position: 326
reference: P85173-1

22579799246975790tt330
type: Position
type: ExactPosition
position: 330
reference: P85173-1

22579799246975790tt331
type: Position
type: ExactPosition
position: 331
reference: P85173-1

22579799246975790tt336
type: Position
type: ExactPosition
position: 336
reference: P85173-1

22579799246975790tt344
type: Position
type: ExactPosition
position: 344
reference: P85173-1

22579799246975790tt354
type: Position
type: ExactPosition
position: 354
reference: P85173-1

22579799246975790tt366
type: Position
type: ExactPosition
position: 366
reference: P85173-1

22579799246975790tt368
type: Position
type: ExactPosition
position: 368
reference: P85173-1

P85173-1
type: Simple Sequence
modified: 2007-07-10
version: 1
mass: 38533
value: DINGGGATLPQKLYLTPDVLTAGFAPYIGVGSGKGKIAFLENKYNQFGTDTTKNVHWAGSDSKLTATELATYAADKEPGWGKLIQVPSVATSVAIPFRKAGANAVDLSVKELCGVFSGRIADWSGITGAGRSGPIQVVYRAESSGTTELFTRFLNAKCTTEPGTFAVTTTFANSYSLGLTPLAGAVAATGSDGVMAALNDTTVAEGRITYISPDFAAPTLAGLDDATKVARVGKGVVNGVAVEGKSPAAANVSAAISVVPLPAAADRGNPDVWVPVFGATTGGGVVAYPDSGYPILGFTNLIFSQCYANATQTGQVRDFFTKHYGTSANNDAAIEANAFVPLPSNWKAAVRASFLTASNALSIGNTNVCNGKGRPQ

http://purl.uniprot.org/uniprot/P85173#attribution-275E9453EBBA3D73DFFCDE319E08C05B
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/16531243

http://purl.uniprot.org/uniprot/P85173#attribution-9299F83AF133DEECE9B8AC275695DED7
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/18076037

http://purl.uniprot.org/uniprot/P85173#attribution-5EEF8344E6EAB0F3FB1BD605DBC8D59F
manual: true
evidence: ECO_0000305

D0E8I4
type: Protein
reviewed: true
created: 2018-10-10
modified: 2023-09-13
version: 30
mnemonic: PHNY_UNCHF
citation: http://purl.uniprot.org/citations/19788654
citation: http://purl.uniprot.org/citations/22564006
recommended name: SIPCC7D8345489E67B1
alternative name: SIP7645FADD4BD69E0C
alternative name: SIP109A128467BE985F
enzyme: http://purl.uniprot.org/enzyme/1.14.11.46
organism: http://purl.uniprot.org/taxonomy/663362
annotation: SIPBC7F0A9EA87687E2
annotation: SIP72420BFA400BC999
annotation: SIP577BCDFDE1AD6B8D
annotation: SIP9724D512D51CD154
annotation: SIP34F9917FB929F116
annotation: PRO_0000445270
annotation: SIP5002D80832AB7FA6
annotation: SIP03DA33685174AE4C
annotation: SIPF7B8B0626C6543C6
annotation: SIP0A24922D0920599D
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/223
classified with: http://purl.uniprot.org/keywords/408
classified with: http://purl.uniprot.org/keywords/479
classified with: GO_0051213
classified with: GO_0046872
sequence: D0E8I4-1

D0E8I4-citation-19788654
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
scope: FUNCTION

D0E8I4-citation-22564006
type: Citation Statement
scope: FUNCTION
scope: CATALYTIC ACTIVITY
scope: ACTIVITY REGULATION
scope: COFACTOR

http://purl.uniprot.org/gene3d/2.60.120.620
type: Resource
database: Gene3D
comment: q2cbj1_9rhob like domain
signature sequence match: Gene3D 2.60.120.620 match 1

IPR008775
type: Resource
database: InterPro
comment: Phytyl_CoA_dOase-like

PTHR20883:SF48
type: Resource
database: PANTHER
comment: ECTOINE DIOXYGENASE
signature sequence match: PANTHER PTHR20883:SF48 match 1

PTHR20883
type: Resource
database: PANTHER
comment: PHYTANOYL-COA DIOXYGENASE DOMAIN CONTAINING 1
signature sequence match: PANTHER PTHR20883 match 1

PF05721
type: Resource
database: Pfam
comment: PhyH
signature sequence match: Pfam PF05721 match 1

SSF51197
type: Resource
database: SUPFAM
comment: Clavaminate synthase-like
signature sequence match: SUPFAM SSF51197 match 1

SIPCC7D8345489E67B1
type: Structured Name
full name: 2-aminoethylphosphonate dioxygenase
ec name: 1.14.11.46

SIP7645FADD4BD69E0C
type: Structured Name
full name: 2-oxoglutarate dioxygensase

SIP109A128467BE985F
type: Structured Name
full name: Alpha-ketoglutarate/Fe(II)-dependent dioxygenase PhnY

phnY
type: Gene
orf name: ALOHA_HF130_AEPn_1_05c

SIPBC7F0A9EA87687E2
type: Function Annotation
comment: Involved in the degradation of the organophosphonate 2-aminoethylphosphonic acid (2-AEP) (Probable). Catalyzes the hydroxylation of 2-aminoethylphosphonic acid to yield (2-amino-1-hydroxyethyl)phosphonic acid (PubMed:22564006).

SIP577BCDFDE1AD6B8D
type: Cofactor Annotation
comment: Binds 1 Fe(2+) ion.
cofactor: CHEBI_29033

SIP9724D512D51CD154
type: Activity Regulation Annotation
comment: Activity is enhanced by ascorbate.

SIP34F9917FB929F116
type: Similarity Annotation
comment: Belongs to the PhyH family.

PRO_0000445270
type: Chain Annotation
comment: 2-aminoethylphosphonate dioxygenase
mass: 30264
range: 19193372271522862tt1tt262

19193372271522862tt1
type: Position
type: ExactPosition
position: 1
reference: D0E8I4-1

19193372271522862tt262
type: Position
type: ExactPosition
position: 262
reference: D0E8I4-1

19193372271522862tt108
type: Position
type: ExactPosition
position: 108
reference: D0E8I4-1

19193372271522862tt118
type: Position
type: ExactPosition
position: 118
reference: D0E8I4-1

19193372271522862tt120
type: Position
type: ExactPosition
position: 120
reference: D0E8I4-1

19193372271522862tt198
type: Position
type: ExactPosition
position: 198
reference: D0E8I4-1

D0E8I4-1
type: Simple Sequence
modified: 2009-11-24
version: 1
mass: 30264
value: MSYFTQEQKTQWKDNGFVHLKGFLNEALAQDIKDWTQELYEWEEAPGKWMKYFETSSDTGERLLCRVENFIDYHKGIKGFLCGEMIYGMVSELMGEQAVLFKEKINFKYPGGAGFAYHQDAPAFTSFGQKYHITMMVSVDASNEENGCLRMAHGFSEEKTLEQEPDGTVCKKLAAKLDWRPLETGPGDLVLFNSYVPHYSEANTSDRSRRAMFITYNRLSEGEKRLDYFKDKREKFPPEAERIEGKDYSSAESLYNLGNPIK

http://purl.uniprot.org/uniprot/D0E8I4#attribution-C06FA86BEFA9CE06DEFE31912D08E4CD
manual: true
evidence: ECO_0000250
source: Q1GNW5

http://purl.uniprot.org/uniprot/D0E8I4#attribution-B9B045FEB264907B7507550445C21DA0
manual: true
evidence: ECO_0000250
source: Q2TDY4

http://purl.uniprot.org/uniprot/D0E8I4#attribution-116377C09FC58E6D64345232D44D6625
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/22564006

http://purl.uniprot.org/uniprot/D0E8I4#attribution-32714BBED54ECA152375A0DF92C00999
manual: true
evidence: ECO_0000303
source: http://purl.uniprot.org/citations/19788654

http://purl.uniprot.org/uniprot/D0E8I4#attribution-31158EFF3C787E7F4608E09A767223D6
manual: true
evidence: ECO_0000303
source: http://purl.uniprot.org/citations/22564006

http://purl.uniprot.org/uniprot/D0E8I4#attribution-D1A8DA7F3200A4C02090409D70497C9E
manual: true
evidence: ECO_0000305

http://purl.uniprot.org/uniprot/D0E8I4#attribution-DDE27361CC69342234D5508DE3D236A4
manual: true
evidence: ECO_0000305
source: http://purl.uniprot.org/citations/19788654

http://purl.uniprot.org/uniprot/D0E8I4#attribution-0A8814E56F3071977C0D21D5B4707F73
manual: true
evidence: ECO_0000312
source: ACU83549.1

D0E8I5
type: Protein
reviewed: true
created: 2018-10-10
modified: 2023-09-13
version: 49
mnemonic: PHNZ_UNCHF
citation: http://purl.uniprot.org/citations/19788654
citation: http://purl.uniprot.org/citations/22564006
citation: http://purl.uniprot.org/citations/24198335
citation: http://purl.uniprot.org/citations/24706911
recommended name: SIP1CA3C484A7AA9195
alternative name: SIP177753C01E735BBA
alternative name: SIP6022E0AE0EF81E79
enzyme: http://purl.uniprot.org/enzyme/1.13.11.78
organism: http://purl.uniprot.org/taxonomy/663362
annotation: SIP02615A52BCE7EF74
annotation: SIP6FA9D6E5B90E5633
annotation: SIP4A7A16CC21CF4404
annotation: SIPB35F72E98D595C4C
annotation: SIP6313AF1D1CC15716
annotation: SIP1080D1447CB27296
annotation: PRO_0000445271
annotation: SIPCC6B2E5F1F87F694
annotation: SIPCAD97BF5AF54EA0D
annotation: SIP0B283737A00D2C51
annotation: SIP792E3F3F85621C6D
annotation: SIP661824E82BF43B03
annotation: SIPC6E076D9F70E478F
annotation: SIP439A3122DDF49D10
annotation: SIP6D5C906124D8EE63
annotation: SIPCAB9DDEEEAD63A7E
annotation: SIP97AFF99C0B5DFB50
annotation: SIP652ABBB07FE9C5EF
annotation: SIP540F78C9F71C7F96
annotation: SIPF5CE674C208CD2C0
annotation: SIPCD34EA2FD80EBDD8
annotation: SIP7A03B246A54248E2
annotation: SIPF21D3440C3806644
annotation: SIP3EE14C9B6698ECD8
annotation: SIP00E444E40116F58F
annotation: SIP92C8F80DAA4D8A80
annotation: SIP65D14BC72B1A2B87
annotation: SIPF6D90CA679B2B5DD
annotation: SIP8C2D6C14701EB56A
annotation: SIPA483CF5A0CCF8671
annotation: SIP2797625C62BD5F11
annotation: SIP8680C6812AE1B2B9
annotation: SIPA12AB0CCCF681646
annotation: SIP7B1817ACB88B3BC3
annotation: SIP3ED9A868246B84EA
annotation: SIP4634AE3CC65BC927
annotation: SIP2244DF24E9BAD7B2
annotation: SIPFD20423FB36FCD50
annotation: SIP6CAEB9122956E3CD
annotation: SIPB46E9D0081CDF9B1
annotation: SIPB62A6DC793E1BB4D
annotation: SIPEAD5E05E483E7A53
annotation: SIPE5F91B014C9B7D67
annotation: SIP2FB10FFBB6430BA3
annotation: SIP91546459C308490D
annotation: SIPDB82023F2181E805
annotation: SIP1C68769623D75D3C
annotation: SIP6B10D6C14C90C652
annotation: SIPA03024FD1EDDA2E7
annotation: SIP4EDA809AB0B01579
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/2
classified with: http://purl.uniprot.org/keywords/378
classified with: http://purl.uniprot.org/keywords/408
classified with: http://purl.uniprot.org/keywords/479
classified with: http://purl.uniprot.org/keywords/560
classified with: GO_0016787
classified with: GO_0046872
classified with: GO_0016491
sequence: MSLSNSSKVSVLISLLEKSRDLDYIGEAINQLEHSLQCAYFAQRSGADNEMVLAALLHDLGHYCNDTSFEDMGGYGVWQHEKVGADYLRGLGFSERVACLIEGHVAAKRYLVSSKASYLKNLSDASRKTLEYQGGPMDEGERRLFEEREDFKDCLKIRAWDEKGKQTDLKVPGPEHYRKMMEEHLSENQN

D0E8I5-citation-19788654
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
scope: FUNCTION

D0E8I5-citation-22564006
type: Citation Statement
scope: FUNCTION
scope: CATALYTIC ACTIVITY
scope: COFACTOR
scope: ACTIVITY REGULATION
scope: REACTION MECHANISM

D0E8I5-citation-24198335
type: Citation Statement
scope: X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS) IN COMPLEX WITH IRON IONS AND SUBSTRATE
scope: FUNCTION
scope: CATALYTIC ACTIVITY
scope: COFACTOR
scope: SUBUNIT
scope: REACTION MECHANISM

D0E8I5-citation-24706911
type: Citation Statement
scope: X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS) IN COMPLEX WITH IRON IONS AND SUBSTRATE
scope: FUNCTION
scope: CATALYTIC ACTIVITY
scope: BIOPHYSICOCHEMICAL PROPERTIES
scope: MUTAGENESIS OF TYR-24; GLU-27; HIS-34; HIS-58; ASP-59; HIS-62; HIS-80; HIS-104 AND ASP-161
scope: COFACTOR
scope: SUBUNIT
scope: SUBSTRATE SPECIFICITY
scope: REACTION MECHANISM

4MLM
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 1.70
chain sequence mapping: PDB 4MLM tt1tt190

PDB 4MLM tt1tt190
chain: A/B=1-190

4MLN
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.10
chain sequence mapping: PDB 4MLN tt1tt190

PDB 4MLN tt1tt190
chain: A/B=1-190

4N6W
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 1.85
chain sequence mapping: PDB 4N6W tt1tt190

PDB 4N6W tt1tt190
chain: A=1-190

4N71
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.98
chain sequence mapping: PDB 4N71 tt1tt190

PDB 4N71 tt1tt190
chain: A/B/D/E=1-190

http://purl.uniprot.org/brenda/1.13.11.78
type: Resource
database: BRENDA
comment: 744

cd00077
type: Resource
database: CDD
comment: HDc
signature sequence match: CDD cd00077 match 1

http://purl.uniprot.org/gene3d/1.10.3210.10
type: Resource
database: Gene3D
comment: Hypothetical protein af1432
signature sequence match: Gene3D 1.10.3210.10 match 1

IPR003607
type: Resource
database: InterPro
comment: HD/PDEase_dom

IPR006674
type: Resource
database: InterPro
comment: HD_domain

IPR006675
type: Resource
database: InterPro
comment: HDIG_dom

TIGR00277
type: Resource
database: NCBIfam
comment: HDIG
signature sequence match: NCBIfam TIGR00277 match 1

PTHR40202:SF1
type: Resource
database: PANTHER
comment: HD_DOMAIN DOMAIN-CONTAINING PROTEIN
signature sequence match: PANTHER PTHR40202:SF1 match 1

PF01966
type: Resource
database: Pfam
comment: HD
signature sequence match: Pfam PF01966 match 1

SSF109604
type: Resource
database: SUPFAM
comment: HD-domain/PDEase-like
signature sequence match: SUPFAM SSF109604 match 1

SIP1CA3C484A7AA9195
type: Structured Name
full name: 2-amino-1-hydroxyethylphosphonate dioxygenase (glycine-forming)
ec name: 1.13.11.78

SIP177753C01E735BBA
type: Structured Name
full name: Di-iron oxygenase

SIP6022E0AE0EF81E79
type: Structured Name
full name: Nonheme iron-dependent oxygenase

phnZ
type: Gene
orf name: ALOHA_HF130_AEPn_1_06c

SIP02615A52BCE7EF74
type: Function Annotation
comment: Involved in the degradation of the organophosphonate 2-aminoethylphosphonic acid (2-AEP) (Probable). Catalyzes the cleavage of the carbon-phosphorus bond of (2-amino-1-hydroxyethyl)phosphonic acid to yield glycine and phosphate through an oxidative mechanism (PubMed:22564006, PubMed:24198335, PubMed:24706911). It reacts stereospecifically with the R-enantiomer of (2-amino-1-hydroxyethyl)phosphonic acid and is also able to use (R,R)-2-amino-1-hydroxypropylphosphonate as substrate (PubMed:24706911).

SIP4A7A16CC21CF4404
type: Cofactor Annotation
comment: Binds 2 iron ions per subunit. During catalysis, PhnZ uses a mixed-valent Fe(2+)/Fe(3+) cofactor.
cofactor: CHEBI_24875

SIPB35F72E98D595C4C
type: Activity Regulation Annotation
comment: Inhibited by EDTA.

SIP6313AF1D1CC15716
type: Kinetics Annotation
comment: kcat is 11 min(-1) for (R)-2-amino-1-hydroxyethylphosphonic acid as substrate. kcat is 7 min(-1) for (R,R)-2-amino-1-hydroxypropylphosphonic acid as substrate.
measured affinity: 0.16 mM for (R,R)-2-amino-1-hydroxypropylphosphonic acid
measured affinity: 0.17 mM for (R)-2-amino-1-hydroxyethylphosphonic acid

SIP1080D1447CB27296
type: Subunit Annotation
comment: Monomer.

PRO_0000445271
type: Chain Annotation
comment: 2-amino-1-hydroxyethylphosphonate dioxygenase (glycine-forming)
mass: 21586
range: 19193372271523118tt1tt190

19193372271523118tt1
type: Position
type: ExactPosition
position: 1
reference: MSLSNSSKVSVLISLLEKSRDLDYIGEAINQLEHSLQCAYFAQRSGADNEMVLAALLHDLGHYCNDTSFEDMGGYGVWQHEKVGADYLRGLGFSERVACLIEGHVAAKRYLVSSKASYLKNLSDASRKTLEYQGGPMDEGERRLFEEREDFKDCLKIRAWDEKGKQTDLKVPGPEHYRKMMEEHLSENQN

19193372271523118tt190
type: Position
type: ExactPosition
position: 190
reference: MSLSNSSKVSVLISLLEKSRDLDYIGEAINQLEHSLQCAYFAQRSGADNEMVLAALLHDLGHYCNDTSFEDMGGYGVWQHEKVGADYLRGLGFSERVACLIEGHVAAKRYLVSSKASYLKNLSDASRKTLEYQGGPMDEGERRLFEEREDFKDCLKIRAWDEKGKQTDLKVPGPEHYRKMMEEHLSENQN

19193372271523118tt24
type: Position
type: ExactPosition
position: 24
reference: MSLSNSSKVSVLISLLEKSRDLDYIGEAINQLEHSLQCAYFAQRSGADNEMVLAALLHDLGHYCNDTSFEDMGGYGVWQHEKVGADYLRGLGFSERVACLIEGHVAAKRYLVSSKASYLKNLSDASRKTLEYQGGPMDEGERRLFEEREDFKDCLKIRAWDEKGKQTDLKVPGPEHYRKMMEEHLSENQN

19193372271523118tt27
type: Position
type: ExactPosition
position: 27
reference: MSLSNSSKVSVLISLLEKSRDLDYIGEAINQLEHSLQCAYFAQRSGADNEMVLAALLHDLGHYCNDTSFEDMGGYGVWQHEKVGADYLRGLGFSERVACLIEGHVAAKRYLVSSKASYLKNLSDASRKTLEYQGGPMDEGERRLFEEREDFKDCLKIRAWDEKGKQTDLKVPGPEHYRKMMEEHLSENQN

19193372271523118tt34
type: Position
type: ExactPosition
position: 34
reference: MSLSNSSKVSVLISLLEKSRDLDYIGEAINQLEHSLQCAYFAQRSGADNEMVLAALLHDLGHYCNDTSFEDMGGYGVWQHEKVGADYLRGLGFSERVACLIEGHVAAKRYLVSSKASYLKNLSDASRKTLEYQGGPMDEGERRLFEEREDFKDCLKIRAWDEKGKQTDLKVPGPEHYRKMMEEHLSENQN

19193372271523118tt58
type: Position
type: ExactPosition
position: 58
reference: MSLSNSSKVSVLISLLEKSRDLDYIGEAINQLEHSLQCAYFAQRSGADNEMVLAALLHDLGHYCNDTSFEDMGGYGVWQHEKVGADYLRGLGFSERVACLIEGHVAAKRYLVSSKASYLKNLSDASRKTLEYQGGPMDEGERRLFEEREDFKDCLKIRAWDEKGKQTDLKVPGPEHYRKMMEEHLSENQN

19193372271523118tt59
type: Position
type: ExactPosition
position: 59
reference: MSLSNSSKVSVLISLLEKSRDLDYIGEAINQLEHSLQCAYFAQRSGADNEMVLAALLHDLGHYCNDTSFEDMGGYGVWQHEKVGADYLRGLGFSERVACLIEGHVAAKRYLVSSKASYLKNLSDASRKTLEYQGGPMDEGERRLFEEREDFKDCLKIRAWDEKGKQTDLKVPGPEHYRKMMEEHLSENQN

19193372271523118tt62
type: Position
type: ExactPosition
position: 62
reference: MSLSNSSKVSVLISLLEKSRDLDYIGEAINQLEHSLQCAYFAQRSGADNEMVLAALLHDLGHYCNDTSFEDMGGYGVWQHEKVGADYLRGLGFSERVACLIEGHVAAKRYLVSSKASYLKNLSDASRKTLEYQGGPMDEGERRLFEEREDFKDCLKIRAWDEKGKQTDLKVPGPEHYRKMMEEHLSENQN

19193372271523118tt80
type: Position
type: ExactPosition
position: 80
reference: MSLSNSSKVSVLISLLEKSRDLDYIGEAINQLEHSLQCAYFAQRSGADNEMVLAALLHDLGHYCNDTSFEDMGGYGVWQHEKVGADYLRGLGFSERVACLIEGHVAAKRYLVSSKASYLKNLSDASRKTLEYQGGPMDEGERRLFEEREDFKDCLKIRAWDEKGKQTDLKVPGPEHYRKMMEEHLSENQN

19193372271523118tt104
type: Position
type: ExactPosition
position: 104
reference: MSLSNSSKVSVLISLLEKSRDLDYIGEAINQLEHSLQCAYFAQRSGADNEMVLAALLHDLGHYCNDTSFEDMGGYGVWQHEKVGADYLRGLGFSERVACLIEGHVAAKRYLVSSKASYLKNLSDASRKTLEYQGGPMDEGERRLFEEREDFKDCLKIRAWDEKGKQTDLKVPGPEHYRKMMEEHLSENQN

19193372271523118tt108
type: Position
type: ExactPosition
position: 108
reference: MSLSNSSKVSVLISLLEKSRDLDYIGEAINQLEHSLQCAYFAQRSGADNEMVLAALLHDLGHYCNDTSFEDMGGYGVWQHEKVGADYLRGLGFSERVACLIEGHVAAKRYLVSSKASYLKNLSDASRKTLEYQGGPMDEGERRLFEEREDFKDCLKIRAWDEKGKQTDLKVPGPEHYRKMMEEHLSENQN

19193372271523118tt126
type: Position
type: ExactPosition
position: 126
reference: MSLSNSSKVSVLISLLEKSRDLDYIGEAINQLEHSLQCAYFAQRSGADNEMVLAALLHDLGHYCNDTSFEDMGGYGVWQHEKVGADYLRGLGFSERVACLIEGHVAAKRYLVSSKASYLKNLSDASRKTLEYQGGPMDEGERRLFEEREDFKDCLKIRAWDEKGKQTDLKVPGPEHYRKMMEEHLSENQN

19193372271523118tt133
type: Position
type: ExactPosition
position: 133
reference: MSLSNSSKVSVLISLLEKSRDLDYIGEAINQLEHSLQCAYFAQRSGADNEMVLAALLHDLGHYCNDTSFEDMGGYGVWQHEKVGADYLRGLGFSERVACLIEGHVAAKRYLVSSKASYLKNLSDASRKTLEYQGGPMDEGERRLFEEREDFKDCLKIRAWDEKGKQTDLKVPGPEHYRKMMEEHLSENQN

19193372271523118tt158
type: Position
type: ExactPosition
position: 158
reference: MSLSNSSKVSVLISLLEKSRDLDYIGEAINQLEHSLQCAYFAQRSGADNEMVLAALLHDLGHYCNDTSFEDMGGYGVWQHEKVGADYLRGLGFSERVACLIEGHVAAKRYLVSSKASYLKNLSDASRKTLEYQGGPMDEGERRLFEEREDFKDCLKIRAWDEKGKQTDLKVPGPEHYRKMMEEHLSENQN

19193372271523118tt161
type: Position
type: ExactPosition
position: 161
reference: MSLSNSSKVSVLISLLEKSRDLDYIGEAINQLEHSLQCAYFAQRSGADNEMVLAALLHDLGHYCNDTSFEDMGGYGVWQHEKVGADYLRGLGFSERVACLIEGHVAAKRYLVSSKASYLKNLSDASRKTLEYQGGPMDEGERRLFEEREDFKDCLKIRAWDEKGKQTDLKVPGPEHYRKMMEEHLSENQN

SIPCD34EA2FD80EBDD8
type: Site Annotation
comment: Important for stereospecificity with the R-enantiomer
range: 19193372271523118tt27tt27

SIP7A03B246A54248E2
type: Mutagenesis Annotation
comment: Slight decrease of the catalytic efficiency and of the affinity for (R)-2-amino-1-hydroxyethylphosphonic acid.
substitution: E
range: 19193372271523118tt24tt24

SIPF21D3440C3806644
type: Mutagenesis Annotation
comment: Slight decrease of the catalytic efficiency and of the affinity for (R)-2-amino-1-hydroxyethylphosphonic acid. 3-fold decrease of the Fe-enzyme molar ratios.
substitution: F
range: 19193372271523118tt24tt24

SIP3EE14C9B6698ECD8
type: Mutagenesis Annotation
comment: 4-fold decrease of the catalytic efficiency and 3-fold decrease of the affinity for (R)-2-amino-1-hydroxyethylphosphonic acid. 6-fold decrease of the Fe-enzyme molar ratios.
substitution: A
range: 19193372271523118tt27tt27

SIP00E444E40116F58F
type: Mutagenesis Annotation
comment: Loss of dioxygenase activity.
substitution: A
range: 19193372271523118tt34tt34

SIP92C8F80DAA4D8A80
type: Mutagenesis Annotation
comment: Loss of dioxygenase activity.
substitution: A
range: 19193372271523118tt58tt58

SIP65D14BC72B1A2B87
type: Mutagenesis Annotation
comment: Loss of dioxygenase activity.
substitution: A
range: 19193372271523118tt59tt59

SIPF6D90CA679B2B5DD
type: Mutagenesis Annotation
comment: 6-fold decrease of the catalytic efficiency and 2.5-fold decrease of the affinity for (R)-2-amino-1-hydroxyethylphosphonic acid.
substitution: A
range: 19193372271523118tt62tt62

SIP8C2D6C14701EB56A
type: Mutagenesis Annotation
comment: 3-fold decrease of the catalytic efficiency and 3.5-fold decrease of the affinity for (R)-2-amino-1-hydroxyethylphosphonic acid. 2.5-fold decrease of the Fe-enzyme molar ratios.
substitution: A
range: 19193372271523118tt80tt80

SIPA483CF5A0CCF8671
type: Mutagenesis Annotation
comment: Loss of dioxygenase activity.
substitution: A
range: 19193372271523118tt104tt104

SIP2797625C62BD5F11
type: Mutagenesis Annotation
comment: Loss of dioxygenase activity.
substitution: A
range: 19193372271523118tt161tt161

19193372271523118tt5
type: Position
type: ExactPosition
position: 5
reference: MSLSNSSKVSVLISLLEKSRDLDYIGEAINQLEHSLQCAYFAQRSGADNEMVLAALLHDLGHYCNDTSFEDMGGYGVWQHEKVGADYLRGLGFSERVACLIEGHVAAKRYLVSSKASYLKNLSDASRKTLEYQGGPMDEGERRLFEEREDFKDCLKIRAWDEKGKQTDLKVPGPEHYRKMMEEHLSENQN

19193372271523118tt18
type: Position
type: ExactPosition
position: 18
reference: MSLSNSSKVSVLISLLEKSRDLDYIGEAINQLEHSLQCAYFAQRSGADNEMVLAALLHDLGHYCNDTSFEDMGGYGVWQHEKVGADYLRGLGFSERVACLIEGHVAAKRYLVSSKASYLKNLSDASRKTLEYQGGPMDEGERRLFEEREDFKDCLKIRAWDEKGKQTDLKVPGPEHYRKMMEEHLSENQN

19193372271523118tt19
type: Position
type: ExactPosition
position: 19
reference: MSLSNSSKVSVLISLLEKSRDLDYIGEAINQLEHSLQCAYFAQRSGADNEMVLAALLHDLGHYCNDTSFEDMGGYGVWQHEKVGADYLRGLGFSERVACLIEGHVAAKRYLVSSKASYLKNLSDASRKTLEYQGGPMDEGERRLFEEREDFKDCLKIRAWDEKGKQTDLKVPGPEHYRKMMEEHLSENQN

19193372271523118tt21
type: Position
type: ExactPosition
position: 21
reference: MSLSNSSKVSVLISLLEKSRDLDYIGEAINQLEHSLQCAYFAQRSGADNEMVLAALLHDLGHYCNDTSFEDMGGYGVWQHEKVGADYLRGLGFSERVACLIEGHVAAKRYLVSSKASYLKNLSDASRKTLEYQGGPMDEGERRLFEEREDFKDCLKIRAWDEKGKQTDLKVPGPEHYRKMMEEHLSENQN

19193372271523118tt22
type: Position
type: ExactPosition
position: 22
reference: MSLSNSSKVSVLISLLEKSRDLDYIGEAINQLEHSLQCAYFAQRSGADNEMVLAALLHDLGHYCNDTSFEDMGGYGVWQHEKVGADYLRGLGFSERVACLIEGHVAAKRYLVSSKASYLKNLSDASRKTLEYQGGPMDEGERRLFEEREDFKDCLKIRAWDEKGKQTDLKVPGPEHYRKMMEEHLSENQN

19193372271523118tt25
type: Position
type: ExactPosition
position: 25
reference: MSLSNSSKVSVLISLLEKSRDLDYIGEAINQLEHSLQCAYFAQRSGADNEMVLAALLHDLGHYCNDTSFEDMGGYGVWQHEKVGADYLRGLGFSERVACLIEGHVAAKRYLVSSKASYLKNLSDASRKTLEYQGGPMDEGERRLFEEREDFKDCLKIRAWDEKGKQTDLKVPGPEHYRKMMEEHLSENQN

19193372271523118tt26
type: Position
type: ExactPosition
position: 26
reference: MSLSNSSKVSVLISLLEKSRDLDYIGEAINQLEHSLQCAYFAQRSGADNEMVLAALLHDLGHYCNDTSFEDMGGYGVWQHEKVGADYLRGLGFSERVACLIEGHVAAKRYLVSSKASYLKNLSDASRKTLEYQGGPMDEGERRLFEEREDFKDCLKIRAWDEKGKQTDLKVPGPEHYRKMMEEHLSENQN

19193372271523118tt28
type: Position
type: ExactPosition
position: 28
reference: MSLSNSSKVSVLISLLEKSRDLDYIGEAINQLEHSLQCAYFAQRSGADNEMVLAALLHDLGHYCNDTSFEDMGGYGVWQHEKVGADYLRGLGFSERVACLIEGHVAAKRYLVSSKASYLKNLSDASRKTLEYQGGPMDEGERRLFEEREDFKDCLKIRAWDEKGKQTDLKVPGPEHYRKMMEEHLSENQN

19193372271523118tt31
type: Position
type: ExactPosition
position: 31
reference: MSLSNSSKVSVLISLLEKSRDLDYIGEAINQLEHSLQCAYFAQRSGADNEMVLAALLHDLGHYCNDTSFEDMGGYGVWQHEKVGADYLRGLGFSERVACLIEGHVAAKRYLVSSKASYLKNLSDASRKTLEYQGGPMDEGERRLFEEREDFKDCLKIRAWDEKGKQTDLKVPGPEHYRKMMEEHLSENQN

19193372271523118tt44
type: Position
type: ExactPosition
position: 44
reference: MSLSNSSKVSVLISLLEKSRDLDYIGEAINQLEHSLQCAYFAQRSGADNEMVLAALLHDLGHYCNDTSFEDMGGYGVWQHEKVGADYLRGLGFSERVACLIEGHVAAKRYLVSSKASYLKNLSDASRKTLEYQGGPMDEGERRLFEEREDFKDCLKIRAWDEKGKQTDLKVPGPEHYRKMMEEHLSENQN

19193372271523118tt49
type: Position
type: ExactPosition
position: 49
reference: MSLSNSSKVSVLISLLEKSRDLDYIGEAINQLEHSLQCAYFAQRSGADNEMVLAALLHDLGHYCNDTSFEDMGGYGVWQHEKVGADYLRGLGFSERVACLIEGHVAAKRYLVSSKASYLKNLSDASRKTLEYQGGPMDEGERRLFEEREDFKDCLKIRAWDEKGKQTDLKVPGPEHYRKMMEEHLSENQN

19193372271523118tt57
type: Position
type: ExactPosition
position: 57
reference: MSLSNSSKVSVLISLLEKSRDLDYIGEAINQLEHSLQCAYFAQRSGADNEMVLAALLHDLGHYCNDTSFEDMGGYGVWQHEKVGADYLRGLGFSERVACLIEGHVAAKRYLVSSKASYLKNLSDASRKTLEYQGGPMDEGERRLFEEREDFKDCLKIRAWDEKGKQTDLKVPGPEHYRKMMEEHLSENQN

19193372271523118tt61
type: Position
type: ExactPosition
position: 61
reference: MSLSNSSKVSVLISLLEKSRDLDYIGEAINQLEHSLQCAYFAQRSGADNEMVLAALLHDLGHYCNDTSFEDMGGYGVWQHEKVGADYLRGLGFSERVACLIEGHVAAKRYLVSSKASYLKNLSDASRKTLEYQGGPMDEGERRLFEEREDFKDCLKIRAWDEKGKQTDLKVPGPEHYRKMMEEHLSENQN

19193372271523118tt66
type: Position
type: ExactPosition
position: 66
reference: MSLSNSSKVSVLISLLEKSRDLDYIGEAINQLEHSLQCAYFAQRSGADNEMVLAALLHDLGHYCNDTSFEDMGGYGVWQHEKVGADYLRGLGFSERVACLIEGHVAAKRYLVSSKASYLKNLSDASRKTLEYQGGPMDEGERRLFEEREDFKDCLKIRAWDEKGKQTDLKVPGPEHYRKMMEEHLSENQN

19193372271523118tt72
type: Position
type: ExactPosition
position: 72
reference: MSLSNSSKVSVLISLLEKSRDLDYIGEAINQLEHSLQCAYFAQRSGADNEMVLAALLHDLGHYCNDTSFEDMGGYGVWQHEKVGADYLRGLGFSERVACLIEGHVAAKRYLVSSKASYLKNLSDASRKTLEYQGGPMDEGERRLFEEREDFKDCLKIRAWDEKGKQTDLKVPGPEHYRKMMEEHLSENQN

19193372271523118tt75
type: Position
type: ExactPosition
position: 75
reference: MSLSNSSKVSVLISLLEKSRDLDYIGEAINQLEHSLQCAYFAQRSGADNEMVLAALLHDLGHYCNDTSFEDMGGYGVWQHEKVGADYLRGLGFSERVACLIEGHVAAKRYLVSSKASYLKNLSDASRKTLEYQGGPMDEGERRLFEEREDFKDCLKIRAWDEKGKQTDLKVPGPEHYRKMMEEHLSENQN

19193372271523118tt77
type: Position
type: ExactPosition
position: 77
reference: MSLSNSSKVSVLISLLEKSRDLDYIGEAINQLEHSLQCAYFAQRSGADNEMVLAALLHDLGHYCNDTSFEDMGGYGVWQHEKVGADYLRGLGFSERVACLIEGHVAAKRYLVSSKASYLKNLSDASRKTLEYQGGPMDEGERRLFEEREDFKDCLKIRAWDEKGKQTDLKVPGPEHYRKMMEEHLSENQN

19193372271523118tt91
type: Position
type: ExactPosition
position: 91
reference: MSLSNSSKVSVLISLLEKSRDLDYIGEAINQLEHSLQCAYFAQRSGADNEMVLAALLHDLGHYCNDTSFEDMGGYGVWQHEKVGADYLRGLGFSERVACLIEGHVAAKRYLVSSKASYLKNLSDASRKTLEYQGGPMDEGERRLFEEREDFKDCLKIRAWDEKGKQTDLKVPGPEHYRKMMEEHLSENQN

19193372271523118tt95
type: Position
type: ExactPosition
position: 95
reference: MSLSNSSKVSVLISLLEKSRDLDYIGEAINQLEHSLQCAYFAQRSGADNEMVLAALLHDLGHYCNDTSFEDMGGYGVWQHEKVGADYLRGLGFSERVACLIEGHVAAKRYLVSSKASYLKNLSDASRKTLEYQGGPMDEGERRLFEEREDFKDCLKIRAWDEKGKQTDLKVPGPEHYRKMMEEHLSENQN

19193372271523118tt102
type: Position
type: ExactPosition
position: 102
reference: MSLSNSSKVSVLISLLEKSRDLDYIGEAINQLEHSLQCAYFAQRSGADNEMVLAALLHDLGHYCNDTSFEDMGGYGVWQHEKVGADYLRGLGFSERVACLIEGHVAAKRYLVSSKASYLKNLSDASRKTLEYQGGPMDEGERRLFEEREDFKDCLKIRAWDEKGKQTDLKVPGPEHYRKMMEEHLSENQN

19193372271523118tt114
type: Position
type: ExactPosition
position: 114
reference: MSLSNSSKVSVLISLLEKSRDLDYIGEAINQLEHSLQCAYFAQRSGADNEMVLAALLHDLGHYCNDTSFEDMGGYGVWQHEKVGADYLRGLGFSERVACLIEGHVAAKRYLVSSKASYLKNLSDASRKTLEYQGGPMDEGERRLFEEREDFKDCLKIRAWDEKGKQTDLKVPGPEHYRKMMEEHLSENQN

19193372271523118tt116
type: Position
type: ExactPosition
position: 116
reference: MSLSNSSKVSVLISLLEKSRDLDYIGEAINQLEHSLQCAYFAQRSGADNEMVLAALLHDLGHYCNDTSFEDMGGYGVWQHEKVGADYLRGLGFSERVACLIEGHVAAKRYLVSSKASYLKNLSDASRKTLEYQGGPMDEGERRLFEEREDFKDCLKIRAWDEKGKQTDLKVPGPEHYRKMMEEHLSENQN

19193372271523118tt121
type: Position
type: ExactPosition
position: 121
reference: MSLSNSSKVSVLISLLEKSRDLDYIGEAINQLEHSLQCAYFAQRSGADNEMVLAALLHDLGHYCNDTSFEDMGGYGVWQHEKVGADYLRGLGFSERVACLIEGHVAAKRYLVSSKASYLKNLSDASRKTLEYQGGPMDEGERRLFEEREDFKDCLKIRAWDEKGKQTDLKVPGPEHYRKMMEEHLSENQN

19193372271523118tt124
type: Position
type: ExactPosition
position: 124
reference: MSLSNSSKVSVLISLLEKSRDLDYIGEAINQLEHSLQCAYFAQRSGADNEMVLAALLHDLGHYCNDTSFEDMGGYGVWQHEKVGADYLRGLGFSERVACLIEGHVAAKRYLVSSKASYLKNLSDASRKTLEYQGGPMDEGERRLFEEREDFKDCLKIRAWDEKGKQTDLKVPGPEHYRKMMEEHLSENQN

19193372271523118tt129
type: Position
type: ExactPosition
position: 129
reference: MSLSNSSKVSVLISLLEKSRDLDYIGEAINQLEHSLQCAYFAQRSGADNEMVLAALLHDLGHYCNDTSFEDMGGYGVWQHEKVGADYLRGLGFSERVACLIEGHVAAKRYLVSSKASYLKNLSDASRKTLEYQGGPMDEGERRLFEEREDFKDCLKIRAWDEKGKQTDLKVPGPEHYRKMMEEHLSENQN

19193372271523118tt130
type: Position
type: ExactPosition
position: 130
reference: MSLSNSSKVSVLISLLEKSRDLDYIGEAINQLEHSLQCAYFAQRSGADNEMVLAALLHDLGHYCNDTSFEDMGGYGVWQHEKVGADYLRGLGFSERVACLIEGHVAAKRYLVSSKASYLKNLSDASRKTLEYQGGPMDEGERRLFEEREDFKDCLKIRAWDEKGKQTDLKVPGPEHYRKMMEEHLSENQN

19193372271523118tt132
type: Position
type: ExactPosition
position: 132
reference: MSLSNSSKVSVLISLLEKSRDLDYIGEAINQLEHSLQCAYFAQRSGADNEMVLAALLHDLGHYCNDTSFEDMGGYGVWQHEKVGADYLRGLGFSERVACLIEGHVAAKRYLVSSKASYLKNLSDASRKTLEYQGGPMDEGERRLFEEREDFKDCLKIRAWDEKGKQTDLKVPGPEHYRKMMEEHLSENQN

19193372271523118tt139
type: Position
type: ExactPosition
position: 139
reference: MSLSNSSKVSVLISLLEKSRDLDYIGEAINQLEHSLQCAYFAQRSGADNEMVLAALLHDLGHYCNDTSFEDMGGYGVWQHEKVGADYLRGLGFSERVACLIEGHVAAKRYLVSSKASYLKNLSDASRKTLEYQGGPMDEGERRLFEEREDFKDCLKIRAWDEKGKQTDLKVPGPEHYRKMMEEHLSENQN

19193372271523118tt147
type: Position
type: ExactPosition
position: 147
reference: MSLSNSSKVSVLISLLEKSRDLDYIGEAINQLEHSLQCAYFAQRSGADNEMVLAALLHDLGHYCNDTSFEDMGGYGVWQHEKVGADYLRGLGFSERVACLIEGHVAAKRYLVSSKASYLKNLSDASRKTLEYQGGPMDEGERRLFEEREDFKDCLKIRAWDEKGKQTDLKVPGPEHYRKMMEEHLSENQN

19193372271523118tt151
type: Position
type: ExactPosition
position: 151
reference: MSLSNSSKVSVLISLLEKSRDLDYIGEAINQLEHSLQCAYFAQRSGADNEMVLAALLHDLGHYCNDTSFEDMGGYGVWQHEKVGADYLRGLGFSERVACLIEGHVAAKRYLVSSKASYLKNLSDASRKTLEYQGGPMDEGERRLFEEREDFKDCLKIRAWDEKGKQTDLKVPGPEHYRKMMEEHLSENQN

19193372271523118tt163
type: Position
type: ExactPosition
position: 163
reference: MSLSNSSKVSVLISLLEKSRDLDYIGEAINQLEHSLQCAYFAQRSGADNEMVLAALLHDLGHYCNDTSFEDMGGYGVWQHEKVGADYLRGLGFSERVACLIEGHVAAKRYLVSSKASYLKNLSDASRKTLEYQGGPMDEGERRLFEEREDFKDCLKIRAWDEKGKQTDLKVPGPEHYRKMMEEHLSENQN

19193372271523118tt174
type: Position
type: ExactPosition
position: 174
reference: MSLSNSSKVSVLISLLEKSRDLDYIGEAINQLEHSLQCAYFAQRSGADNEMVLAALLHDLGHYCNDTSFEDMGGYGVWQHEKVGADYLRGLGFSERVACLIEGHVAAKRYLVSSKASYLKNLSDASRKTLEYQGGPMDEGERRLFEEREDFKDCLKIRAWDEKGKQTDLKVPGPEHYRKMMEEHLSENQN

19193372271523118tt177
type: Position
type: ExactPosition
position: 177
reference: MSLSNSSKVSVLISLLEKSRDLDYIGEAINQLEHSLQCAYFAQRSGADNEMVLAALLHDLGHYCNDTSFEDMGGYGVWQHEKVGADYLRGLGFSERVACLIEGHVAAKRYLVSSKASYLKNLSDASRKTLEYQGGPMDEGERRLFEEREDFKDCLKIRAWDEKGKQTDLKVPGPEHYRKMMEEHLSENQN

19193372271523118tt178
type: Position
type: ExactPosition
position: 178
reference: MSLSNSSKVSVLISLLEKSRDLDYIGEAINQLEHSLQCAYFAQRSGADNEMVLAALLHDLGHYCNDTSFEDMGGYGVWQHEKVGADYLRGLGFSERVACLIEGHVAAKRYLVSSKASYLKNLSDASRKTLEYQGGPMDEGERRLFEEREDFKDCLKIRAWDEKGKQTDLKVPGPEHYRKMMEEHLSENQN

19193372271523118tt187
type: Position
type: ExactPosition
position: 187
reference: MSLSNSSKVSVLISLLEKSRDLDYIGEAINQLEHSLQCAYFAQRSGADNEMVLAALLHDLGHYCNDTSFEDMGGYGVWQHEKVGADYLRGLGFSERVACLIEGHVAAKRYLVSSKASYLKNLSDASRKTLEYQGGPMDEGERRLFEEREDFKDCLKIRAWDEKGKQTDLKVPGPEHYRKMMEEHLSENQN

1
subclass of: CHEBI_24875

2
subclass of: CHEBI_24875

MSLSNSSKVSVLISLLEKSRDLDYIGEAINQLEHSLQCAYFAQRSGADNEMVLAALLHDLGHYCNDTSFEDMGGYGVWQHEKVGADYLRGLGFSERVACLIEGHVAAKRYLVSSKASYLKNLSDASRKTLEYQGGPMDEGERRLFEEREDFKDCLKIRAWDEKGKQTDLKVPGPEHYRKMMEEHLSENQN
type: Simple Sequence
modified: 2009-11-24
version: 1
mass: 21586

http://purl.uniprot.org/uniprot/D0E8I5#attribution-84572DB01BA29A33EAE6BF7132DFD354
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/22564006

http://purl.uniprot.org/uniprot/D0E8I5#attribution-5CEB0854D0C55A0F6CB2DF6C44917731
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/24198335

http://purl.uniprot.org/uniprot/D0E8I5#attribution-B834A7A8FEA29A0337A583B5EEC829B0
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/24706911

http://purl.uniprot.org/uniprot/D0E8I5#attribution-32714BBED54ECA152375A0DF92C00999
manual: true
evidence: ECO_0000303
source: http://purl.uniprot.org/citations/19788654

http://purl.uniprot.org/uniprot/D0E8I5#attribution-31158EFF3C787E7F4608E09A767223D6
manual: true
evidence: ECO_0000303
source: http://purl.uniprot.org/citations/22564006

http://purl.uniprot.org/uniprot/D0E8I5#attribution-823E3DB896D07F38843D55A9D2BAE903
manual: true
evidence: ECO_0000303
source: http://purl.uniprot.org/citations/24706911

http://purl.uniprot.org/uniprot/D0E8I5#attribution-DDE27361CC69342234D5508DE3D236A4
manual: true
evidence: ECO_0000305
source: http://purl.uniprot.org/citations/19788654

http://purl.uniprot.org/uniprot/D0E8I5#attribution-B4D65745AA1855259F2FBC89F714233A
manual: true
evidence: ECO_0000312
source: ACU83550.1

http://purl.uniprot.org/uniprot/D0E8I5#attribution-8F30092BCFB11C4B516ADB5CDD86F19F
manual: true
evidence: ECO_0007744
source: 4MLM

http://purl.uniprot.org/uniprot/D0E8I5#attribution-72E52367B7A97935D9C86DC70410AC71
manual: true
evidence: ECO_0007744
source: 4MLN

http://purl.uniprot.org/uniprot/D0E8I5#attribution-5383043452BED6A155D8A4B9018F966A
manual: true
evidence: ECO_0007744
source: 4N6W

http://purl.uniprot.org/uniprot/D0E8I5#attribution-940FA40DFD7A0B12D3987ECE2F761744
manual: true
evidence: ECO_0007744
source: 4N71

P0DV53
type: Protein
reviewed: true
created: 2022-05-25
modified: 2023-06-28
version: 6
mnemonic: PROT_UNKP
citation: SIP9A1699B052A88F3B
citation: http://purl.uniprot.org/citations/35025633
recommended name: SIPA3B9AE729870090A
alternative name: SIP68517FD9E621AB56
enzyme: http://purl.uniprot.org/enzyme/3.4.21.-
organism: http://purl.uniprot.org/taxonomy/2725
annotation: SIP51F5B43CEC02FE78
annotation: SIP33DA4D1E21167162
annotation: SIPB59F75468D29A8A9
annotation: PRO_0000455580
annotation: SIP9B7B60653A5DAEB6
annotation: SIP676693EA8E5862B6
annotation: SIPD63C3653FC5CD085
annotation: SIP63EA69D52CB3B56C
annotation: SIP672A34E266CDA914
annotation: SIP253FD8C1FF4E4B1D
annotation: SIPCEDC8B830DDBFCC4
annotation: SIPFC4D211821A7A369
annotation: SIPDDD047E46C3E00B4
annotation: SIP54948C4371C3097D
annotation: SIP9B9031C556AA25C6
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/51
classified with: http://purl.uniprot.org/keywords/433
classified with: http://purl.uniprot.org/keywords/677
classified with: http://purl.uniprot.org/keywords/720
classified with: GO_0008236
classified with: GO_0051607
classified with: GO_0006508
sequence: P0DV53-1

P0DV53-citation-SIP9A1699B052A88F3B
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]

P0DV53-citation-35025633
type: Citation Statement
scope: IDENTIFICATION
scope: FUNCTION
scope: BIOPHYSICOCHEMICAL PROPERTIES

http://purl.uniprot.org/gene3d/3.40.50.300
type: Resource
database: Gene3D
comment: P-loop containing nucleotide triphosphate hydrolases
signature sequence match: Gene3D 3.40.50.300 match 1

http://purl.uniprot.org/gene3d/3.80.10.10
type: Resource
database: Gene3D
comment: Ribonuclease Inhibitor
signature sequence match: Gene3D 3.80.10.10 match 1

http://purl.uniprot.org/gene3d/1.10.10.10
type: Resource
database: Gene3D
comment: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
signature sequence match: Gene3D 1.10.10.10 match 1

IPR032171
type: Resource
database: InterPro
comment: COR

IPR045439
type: Resource
database: InterPro
comment: EAD11

IPR001611
type: Resource
database: InterPro
comment: Leu-rich_rpt

IPR003591
type: Resource
database: InterPro
comment: Leu-rich_rpt_typical-subtyp

IPR032675
type: Resource
database: InterPro
comment: LRR_dom_sf

IPR027417
type: Resource
database: InterPro
comment: P-loop_NTPase

IPR036388
type: Resource
database: InterPro
comment: WH-like_DNA-bd_sf

PTHR46652:SF7
type: Resource
database: PANTHER
comment: LEUCINE-RICH REPEAT AND IQ DOMAIN-CONTAINING PROTEIN 1
signature sequence match: PANTHER PTHR46652:SF7 match 1

PTHR46652
type: Resource
database: PANTHER
comment: LEUCINE-RICH REPEAT AND IQ DOMAIN-CONTAINING PROTEIN 1-RELATED
signature sequence match: PANTHER PTHR46652 match 1

PF16095
type: Resource
database: Pfam
comment: COR
signature sequence match: Pfam PF16095 match 1

PF19964
type: Resource
database: Pfam
comment: EAD11
signature sequence match: Pfam PF19964 match 1

PF08477
type: Resource
database: Pfam
comment: Roc
signature sequence match: Pfam PF08477 match 1

PR00449
type: Resource
database: PRINTS
comment: RASTRNSFRMNG

SM00369
type: Resource
database: SMART
comment: LRR_TYP
signature sequence match: SMART SM00369 match 1
signature sequence match: SMART SM00369 match 2
signature sequence match: SMART SM00369 match 3

SSF52058
type: Resource
database: SUPFAM
comment: L domain-like
signature sequence match: SUPFAM SSF52058 match 1

SSF52540
type: Resource
database: SUPFAM
comment: P-loop containing nucleoside triphosphate hydrolases
signature sequence match: SUPFAM SSF52540 match 1

PS51450
type: Resource
database: PROSITE
comment: LRR
signature sequence match: PROSITE PS51450 match 1
signature sequence match: PROSITE PS51450 match 2
signature sequence match: PROSITE PS51450 match 3
signature sequence match: PROSITE PS51450 match 4
signature sequence match: PROSITE PS51450 match 5
signature sequence match: PROSITE PS51450 match 6

SIPA3B9AE729870090A
type: Structured Name
full name: Roc-COR-CHAT protease
ec name: 3.4.21.-

SIP68517FD9E621AB56
type: Structured Name
full name: Protease Ga0307981_100051430

http://purl.uniprot.org/uniprot/P0DV53#gene-MD5904A9D3A917C15035537D096D0C82E87
type: Gene
orf name: Ga0307981_100051430

SIP51F5B43CEC02FE78
type: Function Annotation
comment: A dedicated protease for substrate gasdermin bGSDM; cleaves the bGSDM precursor, releasing the pore-forming moiety, which integrates into the membrane and triggers cell death. Probably involved in defense against bacteriophages. Expression of bGSDM and this neighboring protease is highly toxic in E.coli.

SIP33DA4D1E21167162
type: PH Dependence Annotation
comment: Active between pH 6.0 and 10.2.

SIPB59F75468D29A8A9
type: Annotation
comment: Might be a Bacteriodetes scaffold.

PRO_0000455580
type: Chain Annotation
comment: Roc-COR-CHAT protease
mass: 122028
range: 22571068199088942tt1tt1062

22571068199088942tt1
type: Position
type: ExactPosition
position: 1
reference: P0DV53-1

22571068199088942tt1062
type: Position
type: ExactPosition
position: 1062
reference: P0DV53-1

SIP9B7B60653A5DAEB6
type: Repeat Annotation
comment: LRR 1
range: 22571068199088942tt70tt94

22571068199088942tt70
type: Position
type: ExactPosition
position: 70
reference: P0DV53-1

22571068199088942tt94
type: Position
type: ExactPosition
position: 94
reference: P0DV53-1

SIP676693EA8E5862B6
type: Repeat Annotation
comment: LRR 2
range: 22571068199088942tt95tt116

22571068199088942tt95
type: Position
type: ExactPosition
position: 95
reference: P0DV53-1

22571068199088942tt116
type: Position
type: ExactPosition
position: 116
reference: P0DV53-1

SIPD63C3653FC5CD085
type: Repeat Annotation
comment: LRR 3
range: 22571068199088942tt115tt141

22571068199088942tt115
type: Position
type: ExactPosition
position: 115
reference: P0DV53-1

22571068199088942tt141
type: Position
type: ExactPosition
position: 141
reference: P0DV53-1

SIP63EA69D52CB3B56C
type: Repeat Annotation
comment: LRR 4
range: 22571068199088942tt142tt159

22571068199088942tt142
type: Position
type: ExactPosition
position: 142
reference: P0DV53-1

22571068199088942tt159
type: Position
type: ExactPosition
position: 159
reference: P0DV53-1

SIP672A34E266CDA914
type: Repeat Annotation
comment: LRR 5
range: 22571068199088942tt160tt180

22571068199088942tt160
type: Position
type: ExactPosition
position: 160
reference: P0DV53-1

22571068199088942tt180
type: Position
type: ExactPosition
position: 180
reference: P0DV53-1

SIP253FD8C1FF4E4B1D
type: Repeat Annotation
comment: LRR 6
range: 22571068199088942tt181tt203

22571068199088942tt181
type: Position
type: ExactPosition
position: 181
reference: P0DV53-1

22571068199088942tt203
type: Position
type: ExactPosition
position: 203
reference: P0DV53-1

SIPCEDC8B830DDBFCC4
type: Repeat Annotation
comment: LRR 7
range: 22571068199088942tt204tt226

22571068199088942tt204
type: Position
type: ExactPosition
position: 204
reference: P0DV53-1

22571068199088942tt226
type: Position
type: ExactPosition
position: 226
reference: P0DV53-1

SIPFC4D211821A7A369
type: Repeat Annotation
comment: LRR 8
range: 22571068199088942tt228tt249

22571068199088942tt228
type: Position
type: ExactPosition
position: 228
reference: P0DV53-1

22571068199088942tt249
type: Position
type: ExactPosition
position: 249
reference: P0DV53-1

SIPDDD047E46C3E00B4
type: Region Annotation
comment: Disordered
range: 22571068199088942tt836tt856

22571068199088942tt836
type: Position
type: ExactPosition
position: 836
reference: P0DV53-1

22571068199088942tt856
type: Position
type: ExactPosition
position: 856
reference: P0DV53-1

22571068199088942tt931
type: Position
type: ExactPosition
position: 931
reference: P0DV53-1

22571068199088942tt980
type: Position
type: ExactPosition
position: 980
reference: P0DV53-1

P0DV53-1
type: Simple Sequence
modified: 2022-05-25
version: 1
mass: 122028
value: MPQPAIIAKLEQQLRITIAPFDAPDLKAFMRYGRKGDDNSQYFLKDGKLTGLKLRTLGLKDTSFLEERELAGLQGLYLAENDFSSLQLPGHLQQLRLLHLADNKELKTLEFAGSMPLLEEIDLSDSGIQTLQLPACPALQKLDVSRSKLEAFSFASACPALWWLDLSGNGELRKLKMPAGFKALQYLYLYKSGIQELQINGKLPKLVVLDLEGNQLKQWPEKLLLPEGLETLYLEGNPIENIPETIRGSGERHNSVEDVRQYLLSIIDEDKVEYLHQAKMILVGNGEVGKTSIRLKLLDSKATLPKKKDRTQGLDIVPYELKALSPDLTGLEDAIDFQLNIWDFGGQGKYREVQQLFFSPQSLYLFVTACDDTPEDKESYVTFDYWMSLVHALSYDREQERSSPVIHVVNKIDKERMDIDQTAHNRFGNVEEFHAISCKHLTNFEALRKAIPRVLPKVGQGIFRDQYNEDWLGVMEELQRRQGEHHITYQEYRDEVCKDRLNDGEARAWLRILDRIGTVIYFGENEKLKDWIILNPNWVKQAVFEVIDSGERNPVPQWRFEKQIWSHYSEKEREKLFELLQAYDLAYKQQNAFGEQEFVVPALLEHESPNYQDLLPQEELPLKLRFAFKPILPAGTVNKLMVRLKDYIYRGLMWKDNVVFHHPDSNAYVQVEEDWQEHFIYLSVYGKQPSVIYETVTSTLKDINNDFKNAKFLKELEFTVEGFDGEEWMKKRLLKKAGADFFNFLWEKPGIHYKEEDEKNIMDQVKELIAKNRVGDALEMLKSLVPAHLEAEVLQLISRYSKLQRDSRMGILANNDENVERNRIVSSILNLASEAERDNDHTGLSDSSDQEDETFTNVTDQTKKILFICSSPSGKNLLDFGKEFKSIGIARQLADSRDDYAEPIIKTSVEADDLLHIMTKYQPDILHISLHSSKSKGLYFENNAGQAEPISAEDFKDIIETYASDPDGKGRIETIVLSCCDSEAYGRAITNFADHIVVTKDLFPDKAAVVYAKDFYRMLFNNKDIGFAHRSATSAIKRKKYPSDGFAHPIHEIPFLIKNDDK

http://purl.uniprot.org/uniprot/P0DV53#attribution-ECA57F230B06CF76DEEC761ACB1A4F5F
manual: true
evidence: ECO_0000250
source: P0DV49

http://purl.uniprot.org/uniprot/P0DV53#attribution-E5B7AD25E5784C10C6B9C27D9D877F4E
manual: true
evidence: ECO_0000255

http://purl.uniprot.org/uniprot/P0DV53#attribution-0B651BC8E59F675277B344ADBF95177B
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/35025633

http://purl.uniprot.org/uniprot/P0DV53#attribution-B47C40293467ADABBAA01A15B03AFA9D
manual: true
evidence: ECO_0000303
source: http://purl.uniprot.org/citations/35025633

http://purl.uniprot.org/uniprot/P0DV53#attribution-ABABDC682BFF06AC4515EA431132DCEB
manual: true
evidence: ECO_0000305

http://purl.uniprot.org/uniprot/P0DV53#attribution-5E284D80F5D71292CB5C6BE1AC9E3949
manual: true
evidence: ECO_0000305
source: http://purl.uniprot.org/citations/35025633

Q6J4G7
type: Protein
reviewed: true
created: 2022-02-23
modified: 2023-05-03
version: 53
mnemonic: PRRG_UNKP
citation: http://purl.uniprot.org/citations/15466697
citation: http://purl.uniprot.org/citations/32647827
citation: http://purl.uniprot.org/citations/34226545
recommended name: SIP42C4B6AE57E2652F
organism: http://purl.uniprot.org/taxonomy/2725
annotation: SIP21FCEB69CA83E603
annotation: SIP0B2BF6A03C5A3A8E
annotation: SIPF0B1734AA43ED8D9
annotation: SIPCEC6FB16F682B658
annotation: SIPBDEB9F58A574BFCC
annotation: SIPBDC970BAAB16FDDA
annotation: PRO_5004274813
annotation: SIP3CD2F0C34EC4ACEC
annotation: SIPA63413516B798145
annotation: SIPCB645C0F62730651
annotation: SIP2B97070787A49668
annotation: SIP1D6BC88A264DFC79
annotation: SIP60973735F90F2995
annotation: SIP81EA2EE090CD97DA
annotation: SIPC5E4C09BBE28123E
annotation: SIPFA62F2741EAC5C35
annotation: SIP34D5756301356131
annotation: SIP9A215ED340D7476A
annotation: SIP4C8D55C99577A312
annotation: SIPD71C060983EAD637
annotation: SIP3186B2D9CC8EDC5D
annotation: SIP46CFA5B5BD77012D
annotation: SIP3E01CE2019664743
annotation: SIP49EB13DD82140F30
annotation: SIP5E9EFB1DC1948909
annotation: SIPE222A0E9D8F67F8A
annotation: SIPB20D8C289E1F51F8
annotation: SIP6E3CAF1E66475A2B
annotation: SIP53476D8C99A286B0
annotation: SIPA2D26D3E997CDD44
annotation: SIP41E8F8CA0CD603B4
annotation: SIP13A92B8EA3B24A78
annotation: SIP7B79BC44DE689D5C
annotation: SIP7273470DD9C26CCB
annotation: SIP3553B96ACFEB5F5F
annotation: SIP41EC7DDA05B3E5B6
annotation: SIPA3FA1F63770687CE
annotation: SIP25EC3A830751A00A
annotation: SIP6F3A639B9C480253
annotation: SIP0985CAE51F4774AF
annotation: SIPF03027DE13F21D4E
annotation: SIP68FF1B09516C6D1B
annotation: SIP6B32B9FC9A239620
annotation: SIP2B9B2A6F3D2E56FD
annotation: SIP09A4E10FA2B42ACB
annotation: SIP7E17763ADF2FA52F
annotation: SIPD48A8A1A74C7BC05
annotation: SIPB6C03C61183451D3
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/2
classified with: http://purl.uniprot.org/keywords/1003
classified with: http://purl.uniprot.org/keywords/681
classified with: http://purl.uniprot.org/keywords/732
classified with: http://purl.uniprot.org/keywords/1133
classified with: http://purl.uniprot.org/keywords/813
classified with: GO_0005886
classified with: GO_0010461
classified with: GO_0009881
classified with: GO_0007602
sequence: Q6J4G7-1

Q6J4G7-citation-15466697
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

Q6J4G7-citation-32647827
type: Citation Statement
scope: SUBUNIT

Q6J4G7-citation-34226545
type: Citation Statement
scope: STRUCTURE BY ELECTRON MICROSCOPY (2.93 ANGSTROMS) OF 19-250 IN COVALENT COMPLEX WITH RETINAL
scope: FUNCTION
scope: SUBUNIT
scope: PTM
scope: SUBCELLULAR LOCATION
scope: MUTAGENESIS OF LYS-58; TRP-59; TYR-96; GLU-143; SER-180; TYR-209; TYR-224 AND GLU-246

7B03
type: Structure Resource
database: PDB
method: Electron Microscopy
resolution: 2.93
chain sequence mapping: PDB 7B03 tt19tt250

PDB 7B03 tt19tt250
chain: A/B/C/D/E=19-250

cd15242
type: Resource
database: CDD
comment: 7tm_Proteorhodopsin
signature sequence match: CDD cd15242 match 1

http://purl.uniprot.org/gene3d/1.20.1070.10
type: Resource
database: Gene3D
comment: Rhodopsin 7-helix transmembrane proteins
signature sequence match: Gene3D 1.20.1070.10 match 1

IPR001425
type: Resource
database: InterPro
comment: Arc/bac/fun_rhodopsins

IPR017402
type: Resource
database: InterPro
comment: Proteorhodopsin

IPR018229
type: Resource
database: InterPro
comment: Rhodopsin_retinal_BS

PTHR28286:SF2
type: Resource
database: PANTHER
comment: BACTERIORHODOPSIN /OPSIN, NOPA (EUROFUNG)-RELATED
signature sequence match: PANTHER PTHR28286:SF2 match 1

PF01036
type: Resource
database: Pfam
comment: Bac_rhodopsin
signature sequence match: Pfam PF01036 match 1

PIRSF038142
type: Resource
database: PIRSF
comment: Rhodopsin_bac_prd
signature sequence match: PIRSF PIRSF038142 match 1

SM01021
type: Resource
database: SMART
comment: Bac_rhodopsin
signature sequence match: SMART SM01021 match 1

SSF81321
type: Resource
database: SUPFAM
comment: Family A G protein-coupled receptor-like
signature sequence match: SUPFAM SSF81321 match 1

PS00950
type: Resource
database: PROSITE
comment: BACTERIAL_OPSIN_1
signature sequence match: PROSITE PS00950 match 1

SIP42C4B6AE57E2652F
type: Structured Name
full name: Green-light absorbing proteorhodopsin
short name: GPR

SIP21FCEB69CA83E603
type: Function Annotation
comment: Light-driven proton pump.

SIP0B2BF6A03C5A3A8E
type: Subunit Annotation
comment: Homopentamer (PubMed:34226545, PubMed:32647827). GPR protomers assemble into a pentamer around a central pore with a C5 symmetry axis (PubMed:34226545).

SIPCEC6FB16F682B658
type: PTM Annotation
comment: Contains one covalently linked retinal chromophore per subunit.

SIPBDEB9F58A574BFCC
type: Similarity Annotation
comment: Belongs to the archaeal/bacterial/fungal opsin family.

22859165446125358tt1
type: Position
type: ExactPosition
position: 1
reference: Q6J4G7-1

22859165446125358tt18
type: Position
type: ExactPosition
position: 18
reference: Q6J4G7-1

PRO_5004274813
type: Chain Annotation
comment: Green-light absorbing proteorhodopsin
mass: 25469
range: 22859165446125358tt19tt250

22859165446125358tt19
type: Position
type: ExactPosition
position: 19
reference: Q6J4G7-1

22859165446125358tt250
type: Position
type: ExactPosition
position: 250
reference: Q6J4G7-1

SIP3CD2F0C34EC4ACEC
type: Topological Domain Annotation
comment: Extracellular
range: 22859165446125358tt19tt29

22859165446125358tt29
type: Position
type: ExactPosition
position: 29
reference: Q6J4G7-1

SIPA63413516B798145
type: Transmembrane Annotation
comment: Helical
range: 22859165446125358tt30tt53

22859165446125358tt30
type: Position
type: ExactPosition
position: 30
reference: Q6J4G7-1

22859165446125358tt53
type: Position
type: ExactPosition
position: 53
reference: Q6J4G7-1

SIPCB645C0F62730651
type: Topological Domain Annotation
comment: Cytoplasmic
range: 22859165446125358tt54tt58

22859165446125358tt54
type: Position
type: ExactPosition
position: 54
reference: Q6J4G7-1

22859165446125358tt58
type: Position
type: ExactPosition
position: 58
reference: Q6J4G7-1

SIP2B97070787A49668
type: Transmembrane Annotation
comment: Helical
range: 22859165446125358tt59tt87

22859165446125358tt59
type: Position
type: ExactPosition
position: 59
reference: Q6J4G7-1

22859165446125358tt87
type: Position
type: ExactPosition
position: 87
reference: Q6J4G7-1

SIP1D6BC88A264DFC79
type: Topological Domain Annotation
comment: Extracellular
range: 22859165446125358tt88tt90

22859165446125358tt88
type: Position
type: ExactPosition
position: 88
reference: Q6J4G7-1

22859165446125358tt90
type: Position
type: ExactPosition
position: 90
reference: Q6J4G7-1

SIP60973735F90F2995
type: Transmembrane Annotation
comment: Helical
range: 22859165446125358tt91tt118

22859165446125358tt91
type: Position
type: ExactPosition
position: 91
reference: Q6J4G7-1

22859165446125358tt118
type: Position
type: ExactPosition
position: 118
reference: Q6J4G7-1

SIP81EA2EE090CD97DA
type: Topological Domain Annotation
comment: Cytoplasmic
range: 22859165446125358tt119tt121

22859165446125358tt119
type: Position
type: ExactPosition
position: 119
reference: Q6J4G7-1

22859165446125358tt121
type: Position
type: ExactPosition
position: 121
reference: Q6J4G7-1

SIPC5E4C09BBE28123E
type: Transmembrane Annotation
comment: Helical
range: 22859165446125358tt122tt144

22859165446125358tt122
type: Position
type: ExactPosition
position: 122
reference: Q6J4G7-1

22859165446125358tt144
type: Position
type: ExactPosition
position: 144
reference: Q6J4G7-1

SIPFA62F2741EAC5C35
type: Topological Domain Annotation
comment: Extracellular
range: 22859165446125358tt145tt147

22859165446125358tt145
type: Position
type: ExactPosition
position: 145
reference: Q6J4G7-1

22859165446125358tt147
type: Position
type: ExactPosition
position: 147
reference: Q6J4G7-1

SIP34D5756301356131
type: Transmembrane Annotation
comment: Helical
range: 22859165446125358tt148tt177

22859165446125358tt148
type: Position
type: ExactPosition
position: 148
reference: Q6J4G7-1

22859165446125358tt177
type: Position
type: ExactPosition
position: 177
reference: Q6J4G7-1

SIP9A215ED340D7476A
type: Topological Domain Annotation
comment: Cytoplasmic
range: 22859165446125358tt178tt180

22859165446125358tt178
type: Position
type: ExactPosition
position: 178
reference: Q6J4G7-1

22859165446125358tt180
type: Position
type: ExactPosition
position: 180
reference: Q6J4G7-1

SIP4C8D55C99577A312
type: Transmembrane Annotation
comment: Helical
range: 22859165446125358tt181tt208

22859165446125358tt181
type: Position
type: ExactPosition
position: 181
reference: Q6J4G7-1

22859165446125358tt208
type: Position
type: ExactPosition
position: 208
reference: Q6J4G7-1

SIPD71C060983EAD637
type: Topological Domain Annotation
comment: Extracellular
range: 22859165446125358tt209tt218

22859165446125358tt209
type: Position
type: ExactPosition
position: 209
reference: Q6J4G7-1

22859165446125358tt218
type: Position
type: ExactPosition
position: 218
reference: Q6J4G7-1

SIP3186B2D9CC8EDC5D
type: Transmembrane Annotation
comment: Helical
range: 22859165446125358tt219tt249

22859165446125358tt219
type: Position
type: ExactPosition
position: 219
reference: Q6J4G7-1

22859165446125358tt249
type: Position
type: ExactPosition
position: 249
reference: Q6J4G7-1

SIP46CFA5B5BD77012D
type: Topological Domain Annotation
comment: Cytoplasmic
range: 22859165446125358tt250tt250

SIP3E01CE2019664743
type: Site Annotation
comment: Primary proton acceptor
range: 22859165446125358tt98tt98

22859165446125358tt98
type: Position
type: ExactPosition
position: 98
reference: Q6J4G7-1

SIP49EB13DD82140F30
type: Site Annotation
comment: Responsible for spectral tuning
range: 22859165446125358tt106tt106

22859165446125358tt106
type: Position
type: ExactPosition
position: 106
reference: Q6J4G7-1

SIP5E9EFB1DC1948909
type: Site Annotation
comment: Primary proton donor
range: 22859165446125358tt109tt109

22859165446125358tt109
type: Position
type: ExactPosition
position: 109
reference: Q6J4G7-1

SIPE222A0E9D8F67F8A
type: Site Annotation
comment: Proton release group
range: 22859165446125358tt143tt143

22859165446125358tt143
type: Position
type: ExactPosition
position: 143
reference: Q6J4G7-1

SIPB20D8C289E1F51F8
type: Modified Residue Annotation
comment: N6-(retinylidene)lysine
range: 22859165446125358tt232tt232

22859165446125358tt232
type: Position
type: ExactPosition
position: 232
reference: Q6J4G7-1

SIP6E3CAF1E66475A2B
type: Mutagenesis Annotation
comment: Reduced GPR photoactivity by about 30%.
substitution: A
range: 22859165446125358tt58tt58

SIP53476D8C99A286B0
type: Mutagenesis Annotation
comment: Reduced GPR photoactivity by about 50%.
substitution: A
range: 22859165446125358tt59tt59

SIPA2D26D3E997CDD44
type: Mutagenesis Annotation
comment: Reduced GPR photoactivity by about 50%.
substitution: F
range: 22859165446125358tt96tt96

22859165446125358tt96
type: Position
type: ExactPosition
position: 96
reference: Q6J4G7-1

SIP41E8F8CA0CD603B4
type: Mutagenesis Annotation
comment: Reduced GPR photoactivity by about 50%.
substitution: A
range: 22859165446125358tt143tt143

SIP13A92B8EA3B24A78
type: Mutagenesis Annotation
comment: Increased GPR photoactivity.
substitution: A
range: 22859165446125358tt180tt180

SIP7B79BC44DE689D5C
type: Mutagenesis Annotation
comment: Reduced GPR photoactivity by about 50%.
substitution: F
range: 22859165446125358tt209tt209

SIP7273470DD9C26CCB
type: Mutagenesis Annotation
comment: Reduced GPR photoactivity by about 50%.
substitution: F
range: 22859165446125358tt224tt224

22859165446125358tt224
type: Position
type: ExactPosition
position: 224
reference: Q6J4G7-1

SIP3553B96ACFEB5F5F
type: Mutagenesis Annotation
comment: Increased GPR photoactivity.
substitution: A
range: 22859165446125358tt246tt246

22859165446125358tt246
type: Position
type: ExactPosition
position: 246
reference: Q6J4G7-1

22859165446125358tt27
type: Position
type: ExactPosition
position: 27
reference: Q6J4G7-1

22859165446125358tt31
type: Position
type: ExactPosition
position: 31
reference: Q6J4G7-1

22859165446125358tt50
type: Position
type: ExactPosition
position: 50
reference: Q6J4G7-1

22859165446125358tt51
type: Position
type: ExactPosition
position: 51
reference: Q6J4G7-1

22859165446125358tt57
type: Position
type: ExactPosition
position: 57
reference: Q6J4G7-1

22859165446125358tt86
type: Position
type: ExactPosition
position: 86
reference: Q6J4G7-1

22859165446125358tt92
type: Position
type: ExactPosition
position: 92
reference: Q6J4G7-1

22859165446125358tt115
type: Position
type: ExactPosition
position: 115
reference: Q6J4G7-1

22859165446125358tt123
type: Position
type: ExactPosition
position: 123
reference: Q6J4G7-1

22859165446125358tt149
type: Position
type: ExactPosition
position: 149
reference: Q6J4G7-1

22859165446125358tt168
type: Position
type: ExactPosition
position: 168
reference: Q6J4G7-1

22859165446125358tt171
type: Position
type: ExactPosition
position: 171
reference: Q6J4G7-1

22859165446125358tt176
type: Position
type: ExactPosition
position: 176
reference: Q6J4G7-1

22859165446125358tt196
type: Position
type: ExactPosition
position: 196
reference: Q6J4G7-1

22859165446125358tt197
type: Position
type: ExactPosition
position: 197
reference: Q6J4G7-1

22859165446125358tt199
type: Position
type: ExactPosition
position: 199
reference: Q6J4G7-1

22859165446125358tt200
type: Position
type: ExactPosition
position: 200
reference: Q6J4G7-1

22859165446125358tt220
type: Position
type: ExactPosition
position: 220
reference: Q6J4G7-1

22859165446125358tt234
type: Position
type: ExactPosition
position: 234
reference: Q6J4G7-1

22859165446125358tt248
type: Position
type: ExactPosition
position: 248
reference: Q6J4G7-1

Q6J4G7-1
type: Simple Sequence
modified: 2004-07-05
version: 1
precursor: true
mass: 27308
value: MGKLLLILGSVIALPTFAAGGGDLDASDYTGVSFWLVTAALLASTVFFFVERDRVSAKWKTSLTVSGLVTGIAFWHYMYMRGVWIETGDSPTVFRYIDWLLTVPLLICEFYLILAAATNVAGSLFKKLLVGSLVMLVFGYMGEAGIMAAWPAFIIGCLAWVYMIYELWAGEGKSACNTASPAVQSAYNTMMYIIIFGWAIYPVGYFTGYLMGDGGSALNLNLIYNLADFVNKILFGLIIWNVAVKESSNA

http://purl.uniprot.org/uniprot/Q6J4G7#attribution-FCDA54EE02A944C5909465C8F9F3F16F
manual: true
evidence: ECO_0000255

http://purl.uniprot.org/uniprot/Q6J4G7#attribution-DED3B5159BC66691D6659EF4F9F268E3
manual: true
evidence: ECO_0000255
source: PIRSR038142-1

http://purl.uniprot.org/uniprot/Q6J4G7#attribution-A8512395A13B7BA61D0F89C9FE0CF219
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/32647827

http://purl.uniprot.org/uniprot/Q6J4G7#attribution-592B038BDCB00D2C9E66098165C59A3B
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/34226545

http://purl.uniprot.org/uniprot/Q6J4G7#attribution-AC00640395A3EFDA6BA3F2DDBB880D64
manual: true
evidence: ECO_0000303
source: http://purl.uniprot.org/citations/34226545

http://purl.uniprot.org/uniprot/Q6J4G7#attribution-CC9E613609F5D1C6B1ED8679A65F170F
manual: true
evidence: ECO_0000305

http://purl.uniprot.org/uniprot/Q6J4G7#attribution-D9AEE2D52F4BB9BB87D2E53EEABBF3C0
manual: true
evidence: ECO_0000305
source: http://purl.uniprot.org/citations/34226545

http://purl.uniprot.org/uniprot/Q6J4G7#attribution-6063C7E143BAF99513DB30A8C1361F4D
manual: true
evidence: ECO_0007744
source: 7B03

Q1EIR0
type: Protein
reviewed: true
created: 2017-07-05
modified: 2023-09-13
version: 47
mnemonic: PURNU_UNKP
old mnemonic: POLOX_UNKP
citation: http://purl.uniprot.org/citations/16740638
recommended name: SIP862EED5F6B3C33E7
alternative name: SIP9A939F057506D54C
alternative name: SIPDC7255FC95F9B830
alternative name: SIP97C0005871E8D567
alternative name: SIP512AD54CB025B1CF
alternative name: SIPD8500904ECB08A70
enzyme: http://purl.uniprot.org/enzyme/3.5.4.4
enzyme: http://purl.uniprot.org/enzyme/1.10.3.-
enzyme: http://purl.uniprot.org/enzyme/2.4.2.1
enzyme: http://purl.uniprot.org/enzyme/2.4.2.28
organism: http://purl.uniprot.org/taxonomy/2725
annotation: SIPAF59236459A4D875
annotation: SIPFE0D6B5178B081DF
annotation: SIP6BD44785188F07DA
annotation: SIPC053B90DB6BC1F17
annotation: SIPA30503BDADB976D8
annotation: SIP16ED7B723E3E08A1
annotation: SIP12355F149355EF96
annotation: SIP6A44CA89F9B1B3CA
annotation: SIPAEB637397CB0FE9A
annotation: SIPD5288C7D80C7E263
annotation: SIPA485D9A76F57F619
annotation: PRO_0000440780
annotation: SIP246A7538DEB79131
annotation: SIP863EBCD2765EB183
annotation: SIP3C46ABA9EED56665
annotation: SIPED7528B65F64E500
annotation: SIPDBF27077869D2399
annotation: SIP13D9E4FEC9293A69
annotation: SIP7064F1BD3AF20845
annotation: SIP0EDA44A8D9583DDD
annotation: SIPE932079C452B68AD
annotation: SIP8A2C98933441363C
annotation: SIPBC390927452B79C1
annotation: SIP02A8FF110DCCF527
annotation: SIPD0CB7AE1A8822BB9
annotation: SIPFEEACE20D843D5AA
annotation: SIP570FC8F6B6D5E010
annotation: SIP62B006F8633FE4DD
annotation: SIP3C5CA50C31D64DEB
annotation: SIPE0B9B5DA06B26B8E
annotation: SIP6D0E47CDDA64EB6D
annotation: SIPE0505DED6A18BE98
annotation: SIPADC6A217C925609A
annotation: SIP049915E7A3C42E93
annotation: SIP84E353A6703F67EA
annotation: SIP9CE6DBC98F1671FC
annotation: SIPCE5A2BCBFA460936
annotation: SIP03304ECD00C308DE
annotation: SIP7923C4480B9313FE
annotation: SIPC19C46350C1C759B
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/186
classified with: http://purl.uniprot.org/keywords/378
classified with: http://purl.uniprot.org/keywords/479
classified with: http://purl.uniprot.org/keywords/560
classified with: http://purl.uniprot.org/keywords/808
classified with: http://purl.uniprot.org/keywords/862
classified with: GO_0046936
classified with: GO_0004000
classified with: GO_0005507
classified with: GO_0016682
classified with: GO_0042803
classified with: GO_0017061
sequence: Q1EIR0-1

Q1EIR0-citation-16740638
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
scope: FUNCTION
scope: CATALYTIC ACTIVITY
scope: SUBSTRATE SPECIFICITY
scope: BIOPHYSICOCHEMICAL PROPERTIES
scope: COFACTOR
scope: SUBUNIT
scope: 3D-STRUCTURE MODELING
scope: MUTAGENESIS OF ASN-36; TYR-40; MET-68; HIS-73; CYS-75; ASN-114; CYS-118; HIS-135; CYS-172; CYS-175; HIS-190; HIS-207; HIS-233; CYS-234; CYS-237 AND HIS-239

cd16833
type: Resource
database: CDD
comment: YfiH
signature sequence match: CDD cd16833 match 1

http://purl.uniprot.org/gene3d/3.60.140.10
type: Resource
database: Gene3D
comment: CNF1/YfiH-like putative cysteine hydrolases
signature sequence match: Gene3D 3.60.140.10 match 1

IPR003730
type: Resource
database: InterPro
comment: Cu_polyphenol_OxRdtase

IPR038371
type: Resource
database: InterPro
comment: Cu_polyphenol_OxRdtase_sf

IPR011324
type: Resource
database: InterPro
comment: Cytotoxic_necrot_fac-like_cat

TIGR00726
type: Resource
database: NCBIfam
comment: peptidoglycan editing factor PgeF
signature sequence match: NCBIfam TIGR00726 match 1

PTHR30616:SF2
type: Resource
database: PANTHER
comment: PURINE NUCLEOSIDE PHOSPHORYLASE LACC1
signature sequence match: PANTHER PTHR30616:SF2 match 1

PTHR30616
type: Resource
database: PANTHER
comment: UNCHARACTERIZED PROTEIN YFIH
signature sequence match: PANTHER PTHR30616 match 1

PF02578
type: Resource
database: Pfam
comment: Cu-oxidase_4
signature sequence match: Pfam PF02578 match 1

SSF64438
type: Resource
database: SUPFAM
comment: CNF1/YfiH-like putative cysteine hydrolases
signature sequence match: SUPFAM SSF64438 match 1

SIP862EED5F6B3C33E7
type: Structured Name
full name: Adenosine deaminase RL5
ec name: 3.5.4.4

SIP9A939F057506D54C
type: Structured Name
full name: Laccase RL5

SIPDC7255FC95F9B830
type: Structured Name
full name: Multicopper oxidase RL5

SIP97C0005871E8D567
type: Structured Name
full name: Polyphenol oxidase
ec name: 1.10.3.-

SIP512AD54CB025B1CF
type: Structured Name
full name: Purine nucleoside phosphorylase RL5
ec name: 2.4.2.1

SIPD8500904ECB08A70
type: Structured Name
full name: S-methyl-5'-thioadenosine phosphorylase RL5
ec name: 2.4.2.28

rl5
type: Gene

SIPAF59236459A4D875
type: Function Annotation
comment: Purine nucleoside enzyme that catalyzes the phosphorolysis of adenosine and inosine nucleosides, yielding D-ribose 1-phosphate and the respective free bases, adenine and hypoxanthine (By similarity). Also catalyzes the phosphorolysis of S-methyl-5'-thioadenosine into adenine and S-methyl-5-thio-alpha-D-ribose 1-phosphate (By similarity). Also has adenosine deaminase activity (By similarity). Also acts as a multicopper oxidase able to oxidize a wide variety of polyphenols and related compounds in vitro (PubMed:16740638). Displays substrate preference as follows: syringaldazine > 2,6-dimethoxyphenol > veratryl alcohol > guaiacol > tetramethylbenzidine > 4-methoxybenzyl alcohol > 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) >> phenol red > 1-hydroxybenzotriazole (PubMed:16740638). Cannot use 3,4-dimetoxybenzyl alcohol and violuric acid as substrates (PubMed:16740638). As this enzyme is derived from a rumen microbial community, it may have a role in the digestion of complex plant materials such as ryegrass lignin (PubMed:16740638).

SIP16ED7B723E3E08A1
type: Cofactor Annotation
comment: Binds 4 Cu cations per subunit.
cofactor: CHEBI_23378

SIP12355F149355EF96
type: Kinetics Annotation
comment: kcat is 18 sec(-1) with 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) as substrate. kcat is 660 sec(-1) with syringaldazine as substrate. kcat is 1175 sec(-1) with 2,6-dimethoxyphenol as substrate (at pH 4.5 and 40 degrees Celsius).
measured affinity: 26 uM for 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (at pH 4.5 and 40 degrees Celsius)
measured affinity: 0.43 uM for syringaldazine (at pH 4.5 and 40 degrees Celsius)
measured affinity: 0.45 uM for 2,6-dimethoxyphenol (at pH 4.5 and 40 degrees Celsius)

SIP6A44CA89F9B1B3CA
type: PH Dependence Annotation
comment: Optimum pH is 4.0-5.0. Activity is very high over a broad pH range from 4.0 to 9.0. Exhibits more than 70% activity at pH 3.5 and 9.5.

SIPAEB637397CB0FE9A
type: Temperature Dependence Annotation
comment: Optimum temperature is about 60 degrees Celsius. Is fully stable at this temperature.

SIPD5288C7D80C7E263
type: Subunit Annotation
comment: Homodimer.

SIPA485D9A76F57F619
type: Similarity Annotation
comment: Belongs to the purine nucleoside phosphorylase YfiH/LACC1 family.

PRO_0000440780
type: Chain Annotation
comment: Adenosine deaminase RL5
mass: 28282
range: 22853646766190638tt1tt262

22853646766190638tt1
type: Position
type: ExactPosition
position: 1
reference: Q1EIR0-1

22853646766190638tt262
type: Position
type: ExactPosition
position: 262
reference: Q1EIR0-1

22853646766190638tt36
type: Position
type: ExactPosition
position: 36
reference: Q1EIR0-1

22853646766190638tt40
type: Position
type: ExactPosition
position: 40
reference: Q1EIR0-1

22853646766190638tt68
type: Position
type: ExactPosition
position: 68
reference: Q1EIR0-1

22853646766190638tt73
type: Position
type: ExactPosition
position: 73
reference: Q1EIR0-1

22853646766190638tt75
type: Position
type: ExactPosition
position: 75
reference: Q1EIR0-1

22853646766190638tt114
type: Position
type: ExactPosition
position: 114
reference: Q1EIR0-1

22853646766190638tt118
type: Position
type: ExactPosition
position: 118
reference: Q1EIR0-1

22853646766190638tt135
type: Position
type: ExactPosition
position: 135
reference: Q1EIR0-1

22853646766190638tt172
type: Position
type: ExactPosition
position: 172
reference: Q1EIR0-1

22853646766190638tt175
type: Position
type: ExactPosition
position: 175
reference: Q1EIR0-1

22853646766190638tt234
type: Position
type: ExactPosition
position: 234
reference: Q1EIR0-1

22853646766190638tt237
type: Position
type: ExactPosition
position: 237
reference: Q1EIR0-1

SIPD0CB7AE1A8822BB9
type: Mutagenesis Annotation
comment: Loss of 1.0 of Cu/mol of protein. Loss of catalytic activity.
substitution: A
range: 22853646766190638tt36tt36

SIPFEEACE20D843D5AA
type: Mutagenesis Annotation
comment: Loss of 1.0 of Cu/mol of protein. Loss of catalytic activity.
substitution: A
range: 22853646766190638tt40tt40

SIP570FC8F6B6D5E010
type: Mutagenesis Annotation
comment: No effect on Cu content. No effect on catalytic activity.
substitution: A
range: 22853646766190638tt68tt68

SIP62B006F8633FE4DD
type: Mutagenesis Annotation
comment: Loss of 1.0 of Cu/mol of protein. No effect on secondary structure. Loss of catalytic activity.
substitution: A
range: 22853646766190638tt73tt73

SIP3C5CA50C31D64DEB
type: Mutagenesis Annotation
comment: Loss of 1.0 of Cu/mol of protein. No effect on secondary structure. Loss of catalytic activity.
substitution: Q
range: 22853646766190638tt75tt75

SIPE0B9B5DA06B26B8E
type: Mutagenesis Annotation
comment: Loss of 1.0 of Cu/mol of protein. Loss of catalytic activity.
substitution: A
range: 22853646766190638tt114tt114

SIP6D0E47CDDA64EB6D
type: Mutagenesis Annotation
comment: Loss of 1.0 of Cu/mol of protein. No effect on secondary structure. Loss of catalytic activity.
substitution: Q
range: 22853646766190638tt118tt118

SIPE0505DED6A18BE98
type: Mutagenesis Annotation
comment: Loss of 1.0 of Cu/mol of protein. No effect on secondary structure. Loss of catalytic activity.
substitution: A
range: 22853646766190638tt135tt135

SIPADC6A217C925609A
type: Mutagenesis Annotation
comment: Loss of 1.0 of Cu/mol of protein. Loss of catalytic activity.
substitution: Q
range: 22853646766190638tt172tt172

SIP049915E7A3C42E93
type: Mutagenesis Annotation
comment: Loss of 1.0 of Cu/mol of protein. Loss of catalytic activity.
substitution: Q
range: 22853646766190638tt175tt175

SIP84E353A6703F67EA
type: Mutagenesis Annotation
comment: Binds only 1.0 of Cu/mol of protein. Causes major change in secondary structure. Displays about 10% of wild-type catalytic activity.
substitution: A
range: 22853646766190638tt190tt190

22853646766190638tt190
type: Position
type: ExactPosition
position: 190
reference: Q1EIR0-1

SIP9CE6DBC98F1671FC
type: Mutagenesis Annotation
comment: Binds only 1.0 of Cu/mol of protein. Causes major change in secondary structure. Displays about 10% of wild-type catalytic activity.
substitution: A
range: 22853646766190638tt207tt207

22853646766190638tt207
type: Position
type: ExactPosition
position: 207
reference: Q1EIR0-1

SIPCE5A2BCBFA460936
type: Mutagenesis Annotation
comment: Loss of 1.0 of Cu/mol of protein. No effect on secondary structure. Loss of catalytic activity.
substitution: A
range: 22853646766190638tt233tt233

22853646766190638tt233
type: Position
type: ExactPosition
position: 233
reference: Q1EIR0-1

SIP03304ECD00C308DE
type: Mutagenesis Annotation
comment: No effect on Cu content. No effect on catalytic activity.
substitution: Q
range: 22853646766190638tt234tt234

SIP7923C4480B9313FE
type: Mutagenesis Annotation
comment: Loss of 1.0 of Cu/mol of protein. Loss of catalytic activity.
substitution: Q
range: 22853646766190638tt237tt237

SIPC19C46350C1C759B
type: Mutagenesis Annotation
comment: Binds only 1.0 of Cu/mol of protein. Causes major change in secondary structure. Loss of catalytic activity.
substitution: A
range: 22853646766190638tt239tt239

22853646766190638tt239
type: Position
type: ExactPosition
position: 239
reference: Q1EIR0-1

3
subclass of: CHEBI_23378

1
subclass of: CHEBI_23378
comment: catalytic

2
subclass of: CHEBI_23378

Q1EIR0-1
type: Simple Sequence
modified: 2006-07-11
version: 1
mass: 28282
value: MIELEKLDFAKSVEGVEAFSTTRGQVDGRNAYSGVNLCDYVGDDALRVLDARLTLAMQLGVDLDDLVMPRQTHSCRVAVIDERFRALDIDEQEAALEGVDALVTRLQGIVIGVNTADCVPIVLVDSQAGIVAVSHAGWRGTVGRIAKAVVEEMCRQGATVDRIQAAMGPSICQDCFEVGDEVVEAFKKAHFNLNDIVVRNPATGKAHIDLRAANRAVLVAAGVPAANIVESQHCSRCEHTSFFSARRLGINSGRTFTGIYRK

http://purl.uniprot.org/uniprot/Q1EIR0#attribution-AD98228EF3442FD1FD791F4C2F525B68
creator: UniProtKB
manual: true
evidence: ECO_0000314
source: http://purl.uniprot.org/citations/16740638

http://purl.uniprot.org/uniprot/Q1EIR0#attribution-38B331324D2A238C720EC6B54475EF21
manual: true
evidence: ECO_0000250
source: P84138

http://purl.uniprot.org/uniprot/Q1EIR0#attribution-D4ECC7FF9913DAF8D1E3F29CC8ABB609
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/16740638

http://purl.uniprot.org/uniprot/Q1EIR0#attribution-201A5768114D6BA6B2D692217432FF75
manual: true
evidence: ECO_0000303
source: http://purl.uniprot.org/citations/16740638

http://purl.uniprot.org/uniprot/Q1EIR0#attribution-B3BC3192911C5B6154EA27C798DC8F1F
manual: true
evidence: ECO_0000305

http://purl.uniprot.org/uniprot/Q1EIR0#attribution-D52E28BD61F2F0ABF7C5004DBCD2F3FC
manual: true
evidence: ECO_0000305
source: http://purl.uniprot.org/citations/16740638

http://purl.uniprot.org/uniprot/Q1EIR0#attribution-2E4C96FF3AD0B9727CFBD65EDF516B74
manual: true
evidence: ECO_0000312
source: CAK32504.1

A0A059WYP6
type: Protein
reviewed: true
created: 2019-11-13
modified: 2023-02-22
version: 33
mnemonic: TET50_UNKP
citation: http://purl.uniprot.org/citations/24847883
citation: http://purl.uniprot.org/citations/26097034
citation: http://purl.uniprot.org/citations/28481346
recommended name: SIP0219513550390C3E
alternative name: SIP5E27864609F330BC
enzyme: http://purl.uniprot.org/enzyme/1.14.13.-
organism: http://purl.uniprot.org/taxonomy/2725
annotation: SIP861A10D141F11AC2
annotation: SIP55EE4FAF16967D8B
annotation: SIPBB60DC45708342D6
annotation: SIPC67ABE7167BAD889
annotation: SIP406D12FD06066186
annotation: SIP0C4E62D25E9BA633
annotation: SIP9E2BE0FF1DCBE896
annotation: SIP5B6EE3DC370511A4
annotation: SIP24E7033D9A754574
annotation: SIPF1854F6E1DC7B897
annotation: PRO_0000448491
annotation: SIPE42D29E10C4A45DC
annotation: SIPB7BA3E3BCEDF0D27
annotation: SIPEC99E2F1DB2B3F5D
annotation: SIP9D9C64A6683BC2C6
annotation: SIPC992F38C116198D9
annotation: SIPE88AC07CC148DF11
annotation: SIP65961C600780FB19
annotation: SIPC8E8F3B11EB04CAA
annotation: SIPD7372035AF11AD5B
annotation: SIP831D08B93EF09A2D
annotation: SIP92A86B2B1866DF4E
annotation: SIPF21B8B8989E99AEA
annotation: SIP53FDD08324B806D0
annotation: SIP2489214612C64465
annotation: SIP8778553309BDAE73
annotation: SIP51EE0E581799850D
annotation: SIPC8AEB9D547411B1E
annotation: SIP34829A48CB5E7CC0
annotation: SIP46296796F05F5CA4
annotation: SIPB10DDF6D87DDA599
annotation: SIP960D387C2B9D033D
annotation: SIP7DB9F0314E5E81BE
annotation: SIPE26AC33C28527FAF
annotation: SIPEBDF132D8E138B9E
annotation: SIP403DE2243D086F3D
annotation: SIP44384A7F18F5D790
annotation: SIP54B4C137C55DDD8F
annotation: SIP01F564FB79E7EADB
annotation: SIP90247C24DA1FBA5F
annotation: SIPC79D34D603B8D0EF
annotation: SIPF1C6F138B33FF556
annotation: SIP2AF6D1C81DAB6D78
annotation: SIPE5780BA044A44746
annotation: SIP5C23DC688E176356
annotation: SIP8F01B3CE288E03E4
annotation: SIP5E7B9B5380D22106
annotation: SIP258465D3D6007C89
annotation: SIP21D9AAE524D7B0BF
annotation: SIPD639706F393EE4BB
annotation: SIPF4EF1885CC653B0F
annotation: SIP7D00B9786F1F2970
annotation: SIPF9991204C1EF9D72
annotation: SIPE29B48BCF582776D
annotation: SIP559D7C57474590F7
annotation: SIP20DE473EAC67C8AB
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/2
classified with: http://purl.uniprot.org/keywords/46
classified with: http://purl.uniprot.org/keywords/274
classified with: http://purl.uniprot.org/keywords/521
classified with: http://purl.uniprot.org/keywords/547
classified with: http://purl.uniprot.org/keywords/560
classified with: GO_0071949
classified with: GO_0016491
classified with: GO_0046677
sequence: A0A059WYP6-1

A0A059WYP6-citation-24847883
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

A0A059WYP6-citation-26097034
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
scope: FUNCTION IN INACTIVATING TETRACYCLINE
scope: ANTIBIOTIC RESISTANCE

A0A059WYP6-citation-28481346
type: Citation Statement
scope: X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS) IN COMPLEX WITH FAD ALONE AND IN COMPLEX WITH SUBSTRATE AND INHIBITOR
scope: FUNCTION
scope: SUBSTRATE SPECIFICITY
scope: COFACTOR
scope: ACTIVITY REGULATION
scope: BIOPHYSICOCHEMICAL PROPERTIES
scope: DOMAIN
scope: ANTIBIOTIC RESISTANCE

5TUE
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.10
chain sequence mapping: PDB 5TUE tt1tt388

PDB 5TUE tt1tt388
chain: A/B=1-388

5TUF
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.25
chain sequence mapping: PDB 5TUF tt1tt388

PDB 5TUF tt1tt388
chain: A/B=1-388

5TUI
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 1.75
chain sequence mapping: PDB 5TUI tt1tt388

PDB 5TUI tt1tt388
chain: A/B=1-388

http://purl.uniprot.org/gene3d/3.30.9.10
type: Resource
database: Gene3D
comment: D-Amino Acid Oxidase, subunit A, domain 2
signature sequence match: Gene3D 3.30.9.10 match 1

http://purl.uniprot.org/gene3d/3.50.50.60
type: Resource
database: Gene3D
comment: FAD/NAD(P)-binding domain
signature sequence match: Gene3D 3.50.50.60 match 1

IPR002938
type: Resource
database: InterPro
comment: FAD-bd

IPR036188
type: Resource
database: InterPro
comment: FAD/NAD-bd_sf

PTHR46865
type: Resource
database: PANTHER
comment: OXIDOREDUCTASE-RELATED
signature sequence match: PANTHER PTHR46865 match 1

PTHR46865:SF2
type: Resource
database: PANTHER
comment: POSSIBLE OXIDOREDUCTASE
signature sequence match: PANTHER PTHR46865:SF2 match 1

PF01494
type: Resource
database: Pfam
comment: FAD_binding_3
signature sequence match: Pfam PF01494 match 1

PR00420
type: Resource
database: PRINTS
comment: RNGMNOXGNASE

SSF51905
type: Resource
database: SUPFAM
comment: FAD/NAD(P)-binding domain
signature sequence match: SUPFAM SSF51905 match 1

SIP0219513550390C3E
type: Structured Name
full name: Flavin-dependent monooxygenase

SIP5E27864609F330BC
type: Structured Name
full name: Tetracycline destructase Tet(50)
short name: Tet(50)
ec name: 1.14.13.-

tet(50)
type: Gene

SIP861A10D141F11AC2
type: Function Annotation
comment: An FAD-requiring monooxygenase active on tetracycline antibiotic and some of its derivatives, which leads to their inactivation (PubMed:26097034). Expression in E.coli confers high resistance to tetracycline and oxytetracycline, does not confer resistance to minocycline or tigecycline. Degrades tetracycline and oxytetracycline; the reaction requires NADPH (PubMed:26097034). Degrades and confers resistance to chlortetracycline (PubMed:28481346).

SIPC67ABE7167BAD889
type: Cofactor Annotation
comment: Binds 1 FAD per subunit; it is seen bound in two conformations.
cofactor: CHEBI_57692

SIP406D12FD06066186
type: Activity Regulation Annotation
comment: Inhibited by anhydrotetracycline.

SIP0C4E62D25E9BA633
type: Kinetics Annotation
comment: kcat for tetracycline is 4.3 min(-1), kcat for chlortetracycline is 3.5 min(-1).
measured affinity: 17 uM for tetracycline
measured affinity: 6.3 uM for chlortetracycline

SIP9E2BE0FF1DCBE896
type: Domain Annotation
comment: Consists of an N-terminal FAD-binding domain with a Rossman fold, a substrate-binding domain and a C-terminal helix that bridges the 2 domains close to the antibiotic-binding site. This last helix is flexible, is not found in TetX of Bacteroides species, and may contribute to their different substrate specificities (PubMed:28481346). Binds chlortetracycline in a different orientation (180 degrees rotated on its long axis) compared to tetracycline substrate binding to TetX; binding of chlortetracycline alters conformation FAD and the last helix (PubMed:28481346).

SIP5B6EE3DC370511A4
type: Annotation
comment: Isolated from a grassland soil sample.

SIP24E7033D9A754574
type: Annotation
comment: Tetracycline antibiotics bind to the ribosomal acceptor site (A-site), preventing binding of the aminoacyl-tRNA to the A-site. The hydrophilic side of tetracycline makes many hydrogen-bonding interactions with oxygen atoms of the ribosome's phosphate backbone.

SIPF1854F6E1DC7B897
type: Similarity Annotation
comment: Belongs to the aromatic-ring hydroxylase family.

PRO_0000448491
type: Chain Annotation
comment: Flavin-dependent monooxygenase
mass: 43629
range: -9218586577337837522tt1tt388

-9218586577337837522tt1
type: Position
type: ExactPosition
position: 1
reference: A0A059WYP6-1

-9218586577337837522tt388
type: Position
type: ExactPosition
position: 388
reference: A0A059WYP6-1

-9218586577337837522tt12
type: Position
type: ExactPosition
position: 12
reference: A0A059WYP6-1

-9218586577337837522tt15
type: Position
type: ExactPosition
position: 15
reference: A0A059WYP6-1

-9218586577337837522tt34
type: Position
type: ExactPosition
position: 34
reference: A0A059WYP6-1

-9218586577337837522tt36
type: Position
type: ExactPosition
position: 36
reference: A0A059WYP6-1

-9218586577337837522tt44
type: Position
type: ExactPosition
position: 44
reference: A0A059WYP6-1

-9218586577337837522tt47
type: Position
type: ExactPosition
position: 47
reference: A0A059WYP6-1

-9218586577337837522tt105
type: Position
type: ExactPosition
position: 105
reference: A0A059WYP6-1

-9218586577337837522tt267
type: Position
type: ExactPosition
position: 267
reference: A0A059WYP6-1

-9218586577337837522tt289
type: Position
type: ExactPosition
position: 289
reference: A0A059WYP6-1

-9218586577337837522tt296
type: Position
type: ExactPosition
position: 296
reference: A0A059WYP6-1

-9218586577337837522tt302
type: Position
type: ExactPosition
position: 302
reference: A0A059WYP6-1

-9218586577337837522tt2
type: Position
type: ExactPosition
position: 2
reference: A0A059WYP6-1

-9218586577337837522tt4
type: Position
type: ExactPosition
position: 4
reference: A0A059WYP6-1

-9218586577337837522tt6
type: Position
type: ExactPosition
position: 6
reference: A0A059WYP6-1

-9218586577337837522tt10
type: Position
type: ExactPosition
position: 10
reference: A0A059WYP6-1

-9218586577337837522tt14
type: Position
type: ExactPosition
position: 14
reference: A0A059WYP6-1

-9218586577337837522tt25
type: Position
type: ExactPosition
position: 25
reference: A0A059WYP6-1

-9218586577337837522tt29
type: Position
type: ExactPosition
position: 29
reference: A0A059WYP6-1

-9218586577337837522tt33
type: Position
type: ExactPosition
position: 33
reference: A0A059WYP6-1

-9218586577337837522tt35
type: Position
type: ExactPosition
position: 35
reference: A0A059WYP6-1

-9218586577337837522tt38
type: Position
type: ExactPosition
position: 38
reference: A0A059WYP6-1

-9218586577337837522tt48
type: Position
type: ExactPosition
position: 48
reference: A0A059WYP6-1

-9218586577337837522tt51
type: Position
type: ExactPosition
position: 51
reference: A0A059WYP6-1

-9218586577337837522tt58
type: Position
type: ExactPosition
position: 58
reference: A0A059WYP6-1

-9218586577337837522tt62
type: Position
type: ExactPosition
position: 62
reference: A0A059WYP6-1

-9218586577337837522tt67
type: Position
type: ExactPosition
position: 67
reference: A0A059WYP6-1

-9218586577337837522tt74
type: Position
type: ExactPosition
position: 74
reference: A0A059WYP6-1

-9218586577337837522tt78
type: Position
type: ExactPosition
position: 78
reference: A0A059WYP6-1

-9218586577337837522tt84
type: Position
type: ExactPosition
position: 84
reference: A0A059WYP6-1

-9218586577337837522tt89
type: Position
type: ExactPosition
position: 89
reference: A0A059WYP6-1

-9218586577337837522tt90
type: Position
type: ExactPosition
position: 90
reference: A0A059WYP6-1

-9218586577337837522tt93
type: Position
type: ExactPosition
position: 93
reference: A0A059WYP6-1

-9218586577337837522tt101
type: Position
type: ExactPosition
position: 101
reference: A0A059WYP6-1

-9218586577337837522tt104
type: Position
type: ExactPosition
position: 104
reference: A0A059WYP6-1

-9218586577337837522tt115
type: Position
type: ExactPosition
position: 115
reference: A0A059WYP6-1

-9218586577337837522tt116
type: Position
type: ExactPosition
position: 116
reference: A0A059WYP6-1

-9218586577337837522tt118
type: Position
type: ExactPosition
position: 118
reference: A0A059WYP6-1

-9218586577337837522tt121
type: Position
type: ExactPosition
position: 121
reference: A0A059WYP6-1

-9218586577337837522tt124
type: Position
type: ExactPosition
position: 124
reference: A0A059WYP6-1

-9218586577337837522tt127
type: Position
type: ExactPosition
position: 127
reference: A0A059WYP6-1

-9218586577337837522tt132
type: Position
type: ExactPosition
position: 132
reference: A0A059WYP6-1

-9218586577337837522tt137
type: Position
type: ExactPosition
position: 137
reference: A0A059WYP6-1

-9218586577337837522tt141
type: Position
type: ExactPosition
position: 141
reference: A0A059WYP6-1

-9218586577337837522tt146
type: Position
type: ExactPosition
position: 146
reference: A0A059WYP6-1

-9218586577337837522tt154
type: Position
type: ExactPosition
position: 154
reference: A0A059WYP6-1

-9218586577337837522tt161
type: Position
type: ExactPosition
position: 161
reference: A0A059WYP6-1

-9218586577337837522tt166
type: Position
type: ExactPosition
position: 166
reference: A0A059WYP6-1

-9218586577337837522tt169
type: Position
type: ExactPosition
position: 169
reference: A0A059WYP6-1

-9218586577337837522tt171
type: Position
type: ExactPosition
position: 171
reference: A0A059WYP6-1

-9218586577337837522tt173
type: Position
type: ExactPosition
position: 173
reference: A0A059WYP6-1

-9218586577337837522tt187
type: Position
type: ExactPosition
position: 187
reference: A0A059WYP6-1

-9218586577337837522tt194
type: Position
type: ExactPosition
position: 194
reference: A0A059WYP6-1

-9218586577337837522tt200
type: Position
type: ExactPosition
position: 200
reference: A0A059WYP6-1

-9218586577337837522tt202
type: Position
type: ExactPosition
position: 202
reference: A0A059WYP6-1

-9218586577337837522tt210
type: Position
type: ExactPosition
position: 210
reference: A0A059WYP6-1

-9218586577337837522tt216
type: Position
type: ExactPosition
position: 216
reference: A0A059WYP6-1

-9218586577337837522tt224
type: Position
type: ExactPosition
position: 224
reference: A0A059WYP6-1

-9218586577337837522tt235
type: Position
type: ExactPosition
position: 235
reference: A0A059WYP6-1

-9218586577337837522tt246
type: Position
type: ExactPosition
position: 246
reference: A0A059WYP6-1

-9218586577337837522tt253
type: Position
type: ExactPosition
position: 253
reference: A0A059WYP6-1

-9218586577337837522tt258
type: Position
type: ExactPosition
position: 258
reference: A0A059WYP6-1

-9218586577337837522tt259
type: Position
type: ExactPosition
position: 259
reference: A0A059WYP6-1

-9218586577337837522tt262
type: Position
type: ExactPosition
position: 262
reference: A0A059WYP6-1

-9218586577337837522tt275
type: Position
type: ExactPosition
position: 275
reference: A0A059WYP6-1

-9218586577337837522tt279
type: Position
type: ExactPosition
position: 279
reference: A0A059WYP6-1

-9218586577337837522tt281
type: Position
type: ExactPosition
position: 281
reference: A0A059WYP6-1

-9218586577337837522tt284
type: Position
type: ExactPosition
position: 284
reference: A0A059WYP6-1

-9218586577337837522tt286
type: Position
type: ExactPosition
position: 286
reference: A0A059WYP6-1

-9218586577337837522tt292
type: Position
type: ExactPosition
position: 292
reference: A0A059WYP6-1

-9218586577337837522tt298
type: Position
type: ExactPosition
position: 298
reference: A0A059WYP6-1

-9218586577337837522tt301
type: Position
type: ExactPosition
position: 301
reference: A0A059WYP6-1

-9218586577337837522tt318
type: Position
type: ExactPosition
position: 318
reference: A0A059WYP6-1

-9218586577337837522tt319
type: Position
type: ExactPosition
position: 319
reference: A0A059WYP6-1

-9218586577337837522tt321
type: Position
type: ExactPosition
position: 321
reference: A0A059WYP6-1

-9218586577337837522tt323
type: Position
type: ExactPosition
position: 323
reference: A0A059WYP6-1

-9218586577337837522tt351
type: Position
type: ExactPosition
position: 351
reference: A0A059WYP6-1

-9218586577337837522tt355
type: Position
type: ExactPosition
position: 355
reference: A0A059WYP6-1

-9218586577337837522tt357
type: Position
type: ExactPosition
position: 357
reference: A0A059WYP6-1

-9218586577337837522tt361
type: Position
type: ExactPosition
position: 361
reference: A0A059WYP6-1

-9218586577337837522tt379
type: Position
type: ExactPosition
position: 379
reference: A0A059WYP6-1

A0A059WYP6-1
type: Simple Sequence
modified: 2014-09-03
version: 1
mass: 43629
value: MTKHIKILVIGVGVAGPAVAYWLKRFGFSPVLIEKSAAVRKGGQALDIRGIATHIAKEMGIYDQICNMRTQIKCGRYVDVKGNVLHEEQGETFGFRQDDEVEILRGDLVEILMKAIADIPCEFKQSVIKIEQNEDSVTVTYKDGRVENYDLVIAADGIHSATRGMVFSKNEYQLINLGSYVSAFTIPNYLGLDHMELLCESNHKLVTLQSDSQADKAMAGFMFRSKHVLEDIRDEQEQKHFLHASFQNFGWETQNILNRMPESDDFYFDAITQIKMKSWTKGRIALIGDAAYCPSPLSGQGNNLAFVGAYILAGELKKADGDYIQAFTRYNELLHPFVEANQQFGVWVSESFLLKDDEVSKEIAEARSNKILAMIKSVSNSINLPQYE

http://purl.uniprot.org/uniprot/A0A059WYP6#attribution-3643E26DF670D21B90CF22F583F1B91C
manual: true
evidence: ECO_0000250
source: Q93L51

http://purl.uniprot.org/uniprot/A0A059WYP6#attribution-40EDC338F6A090F55E6EB3CD3ADF06F9
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/26097034

http://purl.uniprot.org/uniprot/A0A059WYP6#attribution-B87DEB5367EE45B6749CA99BECC0D94E
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/28481346

http://purl.uniprot.org/uniprot/A0A059WYP6#attribution-36D5E63425219CD0FA33C0C2A9202C4B
manual: true
evidence: ECO_0000303
source: http://purl.uniprot.org/citations/26097034

http://purl.uniprot.org/uniprot/A0A059WYP6#attribution-4FD6F0CB4D9D232FEB8FE51C9F8FEDB6
manual: true
evidence: ECO_0000305

http://purl.uniprot.org/uniprot/A0A059WYP6#attribution-4EDA16EA9550597AD9C47AADF38A7ECD
manual: true
evidence: ECO_0000305
source: http://purl.uniprot.org/citations/24847883

http://purl.uniprot.org/uniprot/A0A059WYP6#attribution-9A68C307BF7933FD743E787C59D06DE8
manual: true
evidence: ECO_0000305
source: http://purl.uniprot.org/citations/26097034

http://purl.uniprot.org/uniprot/A0A059WYP6#attribution-3046224F0E0C9DAE11EE5ABA232DF3D0
manual: true
evidence: ECO_0000305
source: http://purl.uniprot.org/citations/28481346

http://purl.uniprot.org/uniprot/A0A059WYP6#attribution-02C38133354D3675B5C892EDDC31AF68
manual: true
evidence: ECO_0007744
source: 5TUE

http://purl.uniprot.org/uniprot/A0A059WYP6#attribution-66BB108EDB11569F32FBE90E41D7EDB6
manual: true
evidence: ECO_0007744
source: 5TUF

http://purl.uniprot.org/uniprot/A0A059WYP6#attribution-7E2BDD3A394494B78D3F283E17B45AF4
manual: true
evidence: ECO_0007744
source: 5TUI

A0A0H4TXY1
type: Protein
reviewed: true
created: 2019-11-13
modified: 2023-02-22
version: 26
mnemonic: TET55_UNKP
citation: http://purl.uniprot.org/citations/26097034
citation: http://purl.uniprot.org/citations/28481346
recommended name: SIP0219513550390C3E
alternative name: SIPB7DDE3B7D1552DEE
enzyme: http://purl.uniprot.org/enzyme/1.14.13.-
organism: http://purl.uniprot.org/taxonomy/2725
annotation: SIP5C1B5FE26C48BD1E
annotation: SIP1974E2AE65EE1D31
annotation: SIPBBB857338811F0AE
annotation: SIP778ECC3A707AFE1C
annotation: SIP1734739FC41E9279
annotation: SIP8E4CE87FB69E2D59
annotation: SIP94A0B8222F0D723D
annotation: SIP8B1863A5CFB50C56
annotation: SIP94ABE7F6DFA96CD6
annotation: PRO_0000448492
annotation: SIP93A1A5B0B4BA4F57
annotation: SIPD4D14399B5CBF7A8
annotation: SIPB526295AC7BEA7DE
annotation: SIPFAE3F5090C6D51D5
annotation: SIP20E8EB655D2779F3
annotation: SIP6AD2F9A9F08FEB57
annotation: SIP2F557AA97811CDA0
annotation: SIP331B467DDF61195C
annotation: SIPA1C7352D76E00346
annotation: SIP0976365D18539C35
annotation: SIPDD04EA9F33E5F2E0
annotation: SIP6B7BD4C34D2A041E
annotation: SIPEC6583B500349A34
annotation: SIPBFBE967A471DAACB
annotation: SIPF34222DAC8B97139
annotation: SIP611A66B72EA8EDEA
annotation: SIP01BF8908AFE24817
annotation: SIP83C757A42F968189
annotation: SIPEADE2B4E7FD6128C
annotation: SIPB482256031A8C109
annotation: SIPC325B1DE0865A5C4
annotation: SIPE3216E499A2F918E
annotation: SIP21A6923A108E1A85
annotation: SIP357377D9BF69531D
annotation: SIPB249F59CDB7EED10
annotation: SIP2BD42E2961F5178F
annotation: SIPA5EBA44ECCA2232D
annotation: SIPE88598A96859BA27
annotation: SIPD231D5D64449CF40
annotation: SIPD7F313143E604546
annotation: SIP6FF479FE2A790414
annotation: SIP2D9DDE7EBA9D0C8A
annotation: SIP055A025366FD6861
annotation: SIPCEBF82062C176FEA
annotation: SIP1BD1B9F6BCF6F5F7
annotation: SIP79E63479144BC7BF
annotation: SIPFD15965F5527AFC7
annotation: SIP1CC7445A569C99EF
annotation: SIPC07DFAC394FE2466
annotation: SIP695AF1CD374C028E
annotation: SIP720137E42C26699E
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/2
classified with: http://purl.uniprot.org/keywords/46
classified with: http://purl.uniprot.org/keywords/274
classified with: http://purl.uniprot.org/keywords/521
classified with: http://purl.uniprot.org/keywords/547
classified with: http://purl.uniprot.org/keywords/560
classified with: GO_0071949
classified with: GO_0016491
classified with: GO_0046677
sequence: A0A0H4TXY1-1

A0A0H4TXY1-citation-26097034
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
scope: FUNCTION IN INACTIVATING TETRACYCLINE
scope: ANTIBIOTIC RESISTANCE

A0A0H4TXY1-citation-28481346
type: Citation Statement
scope: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS)
scope: FUNCTION
scope: SUBSTRATE SPECIFICITY
scope: ACTIVITY REGULATION
scope: DOMAIN
scope: ANTIBIOTIC RESISTANCE

5TUL
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.00
chain sequence mapping: PDB 5TUL tt1tt385

PDB 5TUL tt1tt385
chain: A=1-385

SIP0219513550390C3E
type: Structured Name
full name: Flavin-dependent monooxygenase

SIPB7DDE3B7D1552DEE
type: Structured Name
full name: Tetracycline destructase Tet(55)
short name: Tet(55)
ec name: 1.14.13.-

tet(55)
type: Gene

SIP5C1B5FE26C48BD1E
type: Function Annotation
comment: An FAD-requiring monooxygenase active on tetracycline antibiotic and some of its derivatives, which leads to their inactivation (PubMed:26097034). Expression in E.coli confers high resistance to oxytetracycline, slightly less resistance to tetracycline, moderate resistance to minocycline but no resistance to tigecycline. Degrades tetracycline and oxytetracycline; the reaction requires NADPH (PubMed:26097034). Degrades and confers resistance to chlortetracycline (PubMed:28481346).

SIP778ECC3A707AFE1C
type: Cofactor Annotation
comment: Binds 1 FAD per subunit.
cofactor: CHEBI_57692

SIP1734739FC41E9279
type: Activity Regulation Annotation
comment: Inhibited by anhydrotetracycline.

SIP8E4CE87FB69E2D59
type: Domain Annotation
comment: Consists of an N-terminal FAD-binding domain with a Rossman fold, a substrate-binding domain and a C-terminal helix that bridges the 2 domains close to the antibiotic-binding site. This last helix is flexible, is not found in TetX of Bacteroides species, and may contribute to their different substrate specificities.

SIP94A0B8222F0D723D
type: Annotation
comment: Isolated from an agricultural soil sample.

SIP8B1863A5CFB50C56
type: Annotation
comment: Tetracycline antibiotics bind to the ribosomal acceptor site (A-site), preventing binding of the aminoacyl-tRNA to the A-site. The hydrophilic side of tetracycline makes many hydrogen-bonding interactions with oxygen atoms of the ribosome's phosphate backbone.

SIP94ABE7F6DFA96CD6
type: Similarity Annotation
comment: Belongs to the aromatic-ring hydroxylase family.

PRO_0000448492
type: Chain Annotation
comment: Flavin-dependent monooxygenase
mass: 42982
range: -9218585293344848850tt1tt385

-9218585293344848850tt1
type: Position
type: ExactPosition
position: 1
reference: A0A0H4TXY1-1

-9218585293344848850tt385
type: Position
type: ExactPosition
position: 385
reference: A0A0H4TXY1-1

-9218585293344848850tt12
type: Position
type: ExactPosition
position: 12
reference: A0A0H4TXY1-1

-9218585293344848850tt15
type: Position
type: ExactPosition
position: 15
reference: A0A0H4TXY1-1

-9218585293344848850tt34
type: Position
type: ExactPosition
position: 34
reference: A0A0H4TXY1-1

-9218585293344848850tt36
type: Position
type: ExactPosition
position: 36
reference: A0A0H4TXY1-1

-9218585293344848850tt44
type: Position
type: ExactPosition
position: 44
reference: A0A0H4TXY1-1

-9218585293344848850tt47
type: Position
type: ExactPosition
position: 47
reference: A0A0H4TXY1-1

-9218585293344848850tt105
type: Position
type: ExactPosition
position: 105
reference: A0A0H4TXY1-1

-9218585293344848850tt267
type: Position
type: ExactPosition
position: 267
reference: A0A0H4TXY1-1

-9218585293344848850tt289
type: Position
type: ExactPosition
position: 289
reference: A0A0H4TXY1-1

-9218585293344848850tt296
type: Position
type: ExactPosition
position: 296
reference: A0A0H4TXY1-1

-9218585293344848850tt302
type: Position
type: ExactPosition
position: 302
reference: A0A0H4TXY1-1

-9218585293344848850tt6
type: Position
type: ExactPosition
position: 6
reference: A0A0H4TXY1-1

-9218585293344848850tt10
type: Position
type: ExactPosition
position: 10
reference: A0A0H4TXY1-1

-9218585293344848850tt25
type: Position
type: ExactPosition
position: 25
reference: A0A0H4TXY1-1

-9218585293344848850tt29
type: Position
type: ExactPosition
position: 29
reference: A0A0H4TXY1-1

-9218585293344848850tt33
type: Position
type: ExactPosition
position: 33
reference: A0A0H4TXY1-1

-9218585293344848850tt35
type: Position
type: ExactPosition
position: 35
reference: A0A0H4TXY1-1

-9218585293344848850tt38
type: Position
type: ExactPosition
position: 38
reference: A0A0H4TXY1-1

-9218585293344848850tt45
type: Position
type: ExactPosition
position: 45
reference: A0A0H4TXY1-1

-9218585293344848850tt48
type: Position
type: ExactPosition
position: 48
reference: A0A0H4TXY1-1

-9218585293344848850tt51
type: Position
type: ExactPosition
position: 51
reference: A0A0H4TXY1-1

-9218585293344848850tt58
type: Position
type: ExactPosition
position: 58
reference: A0A0H4TXY1-1

-9218585293344848850tt62
type: Position
type: ExactPosition
position: 62
reference: A0A0H4TXY1-1

-9218585293344848850tt67
type: Position
type: ExactPosition
position: 67
reference: A0A0H4TXY1-1

-9218585293344848850tt74
type: Position
type: ExactPosition
position: 74
reference: A0A0H4TXY1-1

-9218585293344848850tt78
type: Position
type: ExactPosition
position: 78
reference: A0A0H4TXY1-1

-9218585293344848850tt84
type: Position
type: ExactPosition
position: 84
reference: A0A0H4TXY1-1

-9218585293344848850tt88
type: Position
type: ExactPosition
position: 88
reference: A0A0H4TXY1-1

-9218585293344848850tt101
type: Position
type: ExactPosition
position: 101
reference: A0A0H4TXY1-1

-9218585293344848850tt104
type: Position
type: ExactPosition
position: 104
reference: A0A0H4TXY1-1

-9218585293344848850tt115
type: Position
type: ExactPosition
position: 115
reference: A0A0H4TXY1-1

-9218585293344848850tt116
type: Position
type: ExactPosition
position: 116
reference: A0A0H4TXY1-1

-9218585293344848850tt118
type: Position
type: ExactPosition
position: 118
reference: A0A0H4TXY1-1

-9218585293344848850tt121
type: Position
type: ExactPosition
position: 121
reference: A0A0H4TXY1-1

-9218585293344848850tt124
type: Position
type: ExactPosition
position: 124
reference: A0A0H4TXY1-1

-9218585293344848850tt127
type: Position
type: ExactPosition
position: 127
reference: A0A0H4TXY1-1

-9218585293344848850tt132
type: Position
type: ExactPosition
position: 132
reference: A0A0H4TXY1-1

-9218585293344848850tt137
type: Position
type: ExactPosition
position: 137
reference: A0A0H4TXY1-1

-9218585293344848850tt141
type: Position
type: ExactPosition
position: 141
reference: A0A0H4TXY1-1

-9218585293344848850tt146
type: Position
type: ExactPosition
position: 146
reference: A0A0H4TXY1-1

-9218585293344848850tt154
type: Position
type: ExactPosition
position: 154
reference: A0A0H4TXY1-1

-9218585293344848850tt161
type: Position
type: ExactPosition
position: 161
reference: A0A0H4TXY1-1

-9218585293344848850tt166
type: Position
type: ExactPosition
position: 166
reference: A0A0H4TXY1-1

-9218585293344848850tt169
type: Position
type: ExactPosition
position: 169
reference: A0A0H4TXY1-1

-9218585293344848850tt171
type: Position
type: ExactPosition
position: 171
reference: A0A0H4TXY1-1

-9218585293344848850tt172
type: Position
type: ExactPosition
position: 172
reference: A0A0H4TXY1-1

-9218585293344848850tt187
type: Position
type: ExactPosition
position: 187
reference: A0A0H4TXY1-1

-9218585293344848850tt193
type: Position
type: ExactPosition
position: 193
reference: A0A0H4TXY1-1

-9218585293344848850tt201
type: Position
type: ExactPosition
position: 201
reference: A0A0H4TXY1-1

-9218585293344848850tt204
type: Position
type: ExactPosition
position: 204
reference: A0A0H4TXY1-1

-9218585293344848850tt210
type: Position
type: ExactPosition
position: 210
reference: A0A0H4TXY1-1

-9218585293344848850tt216
type: Position
type: ExactPosition
position: 216
reference: A0A0H4TXY1-1

-9218585293344848850tt224
type: Position
type: ExactPosition
position: 224
reference: A0A0H4TXY1-1

-9218585293344848850tt235
type: Position
type: ExactPosition
position: 235
reference: A0A0H4TXY1-1

-9218585293344848850tt246
type: Position
type: ExactPosition
position: 246
reference: A0A0H4TXY1-1

-9218585293344848850tt253
type: Position
type: ExactPosition
position: 253
reference: A0A0H4TXY1-1

-9218585293344848850tt259
type: Position
type: ExactPosition
position: 259
reference: A0A0H4TXY1-1

-9218585293344848850tt260
type: Position
type: ExactPosition
position: 260
reference: A0A0H4TXY1-1

-9218585293344848850tt262
type: Position
type: ExactPosition
position: 262
reference: A0A0H4TXY1-1

-9218585293344848850tt277
type: Position
type: ExactPosition
position: 277
reference: A0A0H4TXY1-1

-9218585293344848850tt279
type: Position
type: ExactPosition
position: 279
reference: A0A0H4TXY1-1

-9218585293344848850tt281
type: Position
type: ExactPosition
position: 281
reference: A0A0H4TXY1-1

-9218585293344848850tt284
type: Position
type: ExactPosition
position: 284
reference: A0A0H4TXY1-1

-9218585293344848850tt286
type: Position
type: ExactPosition
position: 286
reference: A0A0H4TXY1-1

-9218585293344848850tt292
type: Position
type: ExactPosition
position: 292
reference: A0A0H4TXY1-1

-9218585293344848850tt298
type: Position
type: ExactPosition
position: 298
reference: A0A0H4TXY1-1

-9218585293344848850tt301
type: Position
type: ExactPosition
position: 301
reference: A0A0H4TXY1-1

-9218585293344848850tt318
type: Position
type: ExactPosition
position: 318
reference: A0A0H4TXY1-1

-9218585293344848850tt319
type: Position
type: ExactPosition
position: 319
reference: A0A0H4TXY1-1

-9218585293344848850tt321
type: Position
type: ExactPosition
position: 321
reference: A0A0H4TXY1-1

-9218585293344848850tt322
type: Position
type: ExactPosition
position: 322
reference: A0A0H4TXY1-1

-9218585293344848850tt350
type: Position
type: ExactPosition
position: 350
reference: A0A0H4TXY1-1

-9218585293344848850tt359
type: Position
type: ExactPosition
position: 359
reference: A0A0H4TXY1-1

-9218585293344848850tt377
type: Position
type: ExactPosition
position: 377
reference: A0A0H4TXY1-1

A0A0H4TXY1-1
type: Simple Sequence
modified: 2015-10-14
version: 1
mass: 42982
value: MPHTKKILVIGASIAGPALCYWLNHYGFQPTLVEKNQSTRKGGYAIDLRGIAVDVAKQMGIYDSVCAMRTSLQCVRYVDAAGNLLFEEHGEKGGFRQGDEVEIVRGDLVDILMKTITDIPCFYDHAIESLTQHDDHVTVQFKNGKTENYDLVIAADGLHSATRRMVFSKDDYHLRNLGCYISVFSIPNYLQLDHCETLLEAKQKLVSITSDKDSTKAFAGFMFRSSNSPNYIRDEASQKDFLRENFTNHGWESNKLLSLMNDANDFYFDAIMQVKMKDWTKGRIALVGDAGYTPSPLSGQGTSLALVGAYILAGELKTATDHVAAFARYNELLKPYVEANQAFGVWVSESFLADEPLSAEQAEERNNIVLGIMKKATHAIELPEY

http://purl.uniprot.org/uniprot/A0A0H4TXY1#attribution-8B9DEADF91F768FA2F29287560A57C3D
manual: true
evidence: ECO_0000250
source: A0A059WYP6

http://purl.uniprot.org/uniprot/A0A0H4TXY1#attribution-3643E26DF670D21B90CF22F583F1B91C
manual: true
evidence: ECO_0000250
source: Q93L51

http://purl.uniprot.org/uniprot/A0A0H4TXY1#attribution-40EDC338F6A090F55E6EB3CD3ADF06F9
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/26097034

http://purl.uniprot.org/uniprot/A0A0H4TXY1#attribution-FDD9B37C704379FBF9BB9021B0631569
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/28481346

http://purl.uniprot.org/uniprot/A0A0H4TXY1#attribution-37D9E9227E357BBA38FF3D3A48C24DA5
manual: true
evidence: ECO_0000303
source: http://purl.uniprot.org/citations/26097034

http://purl.uniprot.org/uniprot/A0A0H4TXY1#attribution-4FD6F0CB4D9D232FEB8FE51C9F8FEDB6
manual: true
evidence: ECO_0000305

http://purl.uniprot.org/uniprot/A0A0H4TXY1#attribution-9A68C307BF7933FD743E787C59D06DE8
manual: true
evidence: ECO_0000305
source: http://purl.uniprot.org/citations/26097034

http://purl.uniprot.org/uniprot/A0A0H4TXY1#attribution-3046224F0E0C9DAE11EE5ABA232DF3D0
manual: true
evidence: ECO_0000305
source: http://purl.uniprot.org/citations/28481346

http://purl.uniprot.org/uniprot/A0A0H4TXY1#attribution-617F234A2C99A6C459AF5B57673EC998
manual: true
evidence: ECO_0007744
source: 5TUL