P30297
type: Protein
reviewed: true
created: 1993-04-01
modified: 2023-09-13
version: 83
mnemonic: 1A1D_PSES0
old mnemonic: A1AD_PSES0
citation: http://purl.uniprot.org/citations/1821764
recommended name: SIP830A7BA9C67F8261
enzyme: http://purl.uniprot.org/enzyme/3.5.99.7
organism: http://purl.uniprot.org/taxonomy/29439
annotation: SIP723D2971AFEA1B61
annotation: SIP6F7BE937D33F4BA2
annotation: SIP411E409D6AADFD1F
annotation: SIP1899C6C9068B9AAF
annotation: SIP198D276109EA4D6C
annotation: SIP71670DAD9B50DEF0
annotation: PRO_0000184503
annotation: SIPE20A4FFF47E0053A
annotation: SIPA13B80DC94896A1F
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/308
classified with: http://purl.uniprot.org/keywords/378
classified with: http://purl.uniprot.org/keywords/663
classified with: GO_0008660
classified with: GO_0030170
classified with: GO_0018871
classified with: GO_0009310
sequence: P30297-1

P30297-citation-1821764
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

cd06449
type: Resource
database: CDD
comment: ACCD
signature sequence match: CDD cd06449 match 1

MF_00807
type: Resource
database: HAMAP
comment: ACC_deaminase
signature sequence match: HAMAP MF 00807 match 1

IPR027278
type: Resource
database: InterPro
comment: ACCD_DCysDesulf

IPR005965
type: Resource
database: InterPro
comment: ACP_carboxylate_deaminase

IPR020601
type: Resource
database: InterPro
comment: ACP_carboxylate_deaminase_bac

IPR001926
type: Resource
database: InterPro
comment: TrpB-like_PALP

IPR036052
type: Resource
database: InterPro
comment: TrpB-like_PALP_sf

TIGR01274
type: Resource
database: NCBIfam
comment: ACC_deam
signature sequence match: NCBIfam TIGR01274 match 1

PTHR43780
type: Resource
database: PANTHER
comment: 1-AMINOCYCLOPROPANE-1-CARBOXYLATE DEAMINASE-RELATED
signature sequence match: PANTHER PTHR43780 match 1

PTHR43780:SF2
type: Resource
database: PANTHER
comment: 1-AMINOCYCLOPROPANE-1-CARBOXYLATE DEAMINASE-RELATED
signature sequence match: PANTHER PTHR43780:SF2 match 1

PF00291
type: Resource
database: Pfam
comment: PALP
signature sequence match: Pfam PF00291 match 1

PIRSF006278
type: Resource
database: PIRSF
comment: ACCD_DCysDesulf
signature sequence match: PIRSF PIRSF006278 match 1

SSF53686
type: Resource
database: SUPFAM
comment: Tryptophan synthase beta subunit-like PLP-dependent enzymes
signature sequence match: SUPFAM SSF53686 match 1

SIP830A7BA9C67F8261
type: Structured Name
full name: 1-aminocyclopropane-1-carboxylate deaminase
short name: ACC deaminase
short name: ACCD
ec name: 3.5.99.7

acdS
type: Gene

SIP723D2971AFEA1B61
type: Function Annotation
comment: Catalyzes a cyclopropane ring-opening reaction, the irreversible conversion of 1-aminocyclopropane-1-carboxylate (ACC) to ammonia and alpha-ketobutyrate. Allows growth on ACC as a nitrogen source.

SIP1899C6C9068B9AAF
type: Subunit Annotation
comment: Homotrimer.

SIP198D276109EA4D6C
type: Biotechnology Annotation
comment: Introduced by genetic manipulation and expressed in improved ripening tomato by Monsanto. ACC is the immediate precursor of the phytohormone ethylene which is involved in the control of ripening. ACC deaminase reduces ethylene biosynthesis and thus extends the shelf life of fruits and vegetables.

SIP71670DAD9B50DEF0
type: Similarity Annotation
comment: Belongs to the ACC deaminase/D-cysteine desulfhydrase family.

PRO_0000184503
type: Chain Annotation
comment: 1-aminocyclopropane-1-carboxylate deaminase
mass: 36873
range: 22574280230909742tt1tt338

22574280230909742tt1
type: Position
type: ExactPosition
position: 1
reference: P30297-1

22574280230909742tt338
type: Position
type: ExactPosition
position: 338
reference: P30297-1

SIPE20A4FFF47E0053A
type: Active Site Annotation
comment: Nucleophile
range: 22574280230909742tt78tt78

22574280230909742tt78
type: Position
type: ExactPosition
position: 78
reference: P30297-1

SIPA13B80DC94896A1F
type: Modified Residue Annotation
comment: N6-(pyridoxal phosphate)lysine
range: 22574280230909742tt51tt51

22574280230909742tt51
type: Position
type: ExactPosition
position: 51
reference: P30297-1

P30297-1
type: Simple Sequence
modified: 1993-04-01
version: 1
mass: 36873
value: MNLNRFERYPLTFGPSPITPLKRLSQHLGGKVELYAKREDCNSGLAFGGNKTRKLEYLIPEAIEQGCDTLVSIGGIQSNQTRQVAAVAAHLGMKCVLVQENWVNYSDAVYDRVGNIEMSRIMGADVRLDAAGFDIGIRPSWEKAMSDVVEQGGKPFPIPAGCSEHPYGGLGFVGFAEEVRQQEKELGFKFDYIVVCSVTGSTQAGMVVGFAADGRSKNVIGIDASAKPEQTKAQILRIARHTAELVELGREITEEDVVLDTRFAYPEYGLPNEGTLEAIRLCGSLEGVLTDPVYEGKSMHGMIEMVRRGEFPEGSKVLYAHLGGAPALNAYSFLFRNG

http://purl.uniprot.org/uniprot/P30297#attribution-EF49CFF7CB7DF66E7BB7BA4F7CE54F55
manual: true
evidence: ECO_0000255
source: MF_00807

Q00740
type: Protein
reviewed: true
created: 1993-04-01
modified: 2023-09-13
version: 95
mnemonic: 1A1D_PSEUD
old mnemonic: 1A1D_PSESP
citation: http://purl.uniprot.org/citations/1885510
recommended name: SIP830A7BA9C67F8261
enzyme: http://purl.uniprot.org/enzyme/3.5.99.7
organism: http://purl.uniprot.org/taxonomy/74568
annotation: SIPBC4765B974B3F2F4
annotation: SIPF1D064A8127660CA
annotation: SIP535B1DD0544D8C4F
annotation: SIP19A70272D44DBAD6
annotation: SIP18D16278380CA15B
annotation: PRO_0000184505
annotation: SIPF14BCB8942D1449A
annotation: SIP72E5392805704D97
annotation: SIPA3C40E22444300C1
annotation: SIPAE13F843055E3593
annotation: SIP0F15FC1368CCA19D
annotation: SIP54F53033774AF79D
annotation: SIP575BB5736106082A
annotation: SIP158F6D83DFC3D049
annotation: SIPA40B0C07C6744978
annotation: SIPCC4505CEAEB0B68D
annotation: SIPB740BCC687071F4E
annotation: SIPEFC3AC47E696F66C
annotation: SIP8896563A59D102E9
annotation: SIPAB2B90FA08CF5D3D
annotation: SIP541651CB96CD25A4
annotation: SIP3BF5FAFED4E12B3A
annotation: SIP749906341274A1DF
annotation: SIP68286B4EE272286A
annotation: SIP19DF85B8F8340BE6
annotation: SIP3E3CC4950BEE037B
annotation: SIP83B3F3ABDF2C0B87
annotation: SIP22D289E7DDDE169A
annotation: SIP242B6161EFDD2914
annotation: SIP52B3693305CFC9BE
annotation: SIPF88FCEE571077859
annotation: SIP48D8529E015DEC6C
annotation: SIP1F30FF5E34A4983D
annotation: SIP41380600F60D47AF
annotation: SIP603EA80AA8D3DC26
annotation: SIP22087BD08A5D55F8
annotation: SIPFE8DE9F3E4EF60CE
annotation: SIPDD27A3C1762D01DB
annotation: SIPF03510A9B0277E74
annotation: SIPD3F84C96BE4F86D1
annotation: SIPB98C8230E27E4BA6
annotation: SIPC8CA2C5970E161E1
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/2
classified with: http://purl.uniprot.org/keywords/903
classified with: http://purl.uniprot.org/keywords/378
classified with: http://purl.uniprot.org/keywords/663
classified with: GO_0008660
classified with: GO_0030170
classified with: GO_0018871
classified with: GO_0009310
sequence: Q00740-1

Q00740-citation-1885510
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
scope: PROTEIN SEQUENCE
scope: FUNCTION
scope: CATALYTIC ACTIVITY
scope: COFACTOR

1RQX
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.50
chain sequence mapping: PDB 1RQX tt1tt338

PDB 1RQX tt1tt338
chain: A/B/C/D=1-338

1TYZ
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.00
chain sequence mapping: PDB 1TYZ tt1tt338

PDB 1TYZ tt1tt338
chain: A/B/C/D=1-338

1TZ2
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.10
chain sequence mapping: PDB 1TZ2 tt1tt338

PDB 1TZ2 tt1tt338
chain: A/B/C/D=1-338

1TZJ
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 1.99
chain sequence mapping: PDB 1TZJ tt1tt338

PDB 1TZJ tt1tt338
chain: A/B/C/D=1-338

1TZK
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.00
chain sequence mapping: PDB 1TZK tt1tt338

PDB 1TZK tt1tt338
chain: A/B/C/D=1-338

1TZM
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.08
chain sequence mapping: PDB 1TZM tt1tt338

PDB 1TZM tt1tt338
chain: A/B/C/D=1-338

DB02085
type: Resource
database: DrugBank
comment: 1-Aminocyclopropanecarboxylic Acid

DB03053
type: Resource
database: DrugBank
comment: 1-Aminocyclopropylphosphonate

DB01804
type: Resource
database: DrugBank
comment: 2-Ammoniobut-3-Enoate, 2-Amino-3-Butenoate

DB04212
type: Resource
database: DrugBank
comment: 2-Iminiopropanoate

DB04553
type: Resource
database: DrugBank
comment: 2-Oxobutanoic Acid

DB01735
type: Resource
database: DrugBank
comment: 3-Chloroalaninate

http://purl.uniprot.org/brenda/3.5.99.7
type: Resource
database: BRENDA
comment: 5085

SIP830A7BA9C67F8261
type: Structured Name
full name: 1-aminocyclopropane-1-carboxylate deaminase
short name: ACC deaminase
short name: ACCD
ec name: 3.5.99.7

acdS
type: Gene

SIPBC4765B974B3F2F4
type: Function Annotation
comment: Catalyzes a cyclopropane ring-opening reaction, the irreversible conversion of 1-aminocyclopropane-1-carboxylate (ACC) to ammonia and alpha-ketobutyrate. Allows growth on ACC as a nitrogen source.

SIP19A70272D44DBAD6
type: Subunit Annotation
comment: Homotrimer.

SIP18D16278380CA15B
type: Similarity Annotation
comment: Belongs to the ACC deaminase/D-cysteine desulfhydrase family.

PRO_0000184505
type: Chain Annotation
comment: 1-aminocyclopropane-1-carboxylate deaminase
mass: 36672
range: 22852456756293678tt1tt338

22852456756293678tt1
type: Position
type: ExactPosition
position: 1
reference: Q00740-1

22852456756293678tt338
type: Position
type: ExactPosition
position: 338
reference: Q00740-1

SIPF14BCB8942D1449A
type: Active Site Annotation
comment: Nucleophile
range: 22852456756293678tt78tt78

22852456756293678tt78
type: Position
type: ExactPosition
position: 78
reference: Q00740-1

SIP72E5392805704D97
type: Modified Residue Annotation
comment: N6-(pyridoxal phosphate)lysine
range: 22852456756293678tt51tt51

22852456756293678tt51
type: Position
type: ExactPosition
position: 51
reference: Q00740-1

22852456756293678tt3
type: Position
type: ExactPosition
position: 3
reference: Q00740-1

22852456756293678tt5
type: Position
type: ExactPosition
position: 5
reference: Q00740-1

22852456756293678tt12
type: Position
type: ExactPosition
position: 12
reference: Q00740-1

22852456756293678tt15
type: Position
type: ExactPosition
position: 15
reference: Q00740-1

22852456756293678tt18
type: Position
type: ExactPosition
position: 18
reference: Q00740-1

22852456756293678tt20
type: Position
type: ExactPosition
position: 20
reference: Q00740-1

22852456756293678tt22
type: Position
type: ExactPosition
position: 22
reference: Q00740-1

22852456756293678tt27
type: Position
type: ExactPosition
position: 27
reference: Q00740-1

22852456756293678tt30
type: Position
type: ExactPosition
position: 30
reference: Q00740-1

22852456756293678tt38
type: Position
type: ExactPosition
position: 38
reference: Q00740-1

22852456756293678tt39
type: Position
type: ExactPosition
position: 39
reference: Q00740-1

22852456756293678tt41
type: Position
type: ExactPosition
position: 41
reference: Q00740-1

22852456756293678tt50
type: Position
type: ExactPosition
position: 50
reference: Q00740-1

22852456756293678tt56
type: Position
type: ExactPosition
position: 56
reference: Q00740-1

22852456756293678tt59
type: Position
type: ExactPosition
position: 59
reference: Q00740-1

22852456756293678tt64
type: Position
type: ExactPosition
position: 64
reference: Q00740-1

22852456756293678tt69
type: Position
type: ExactPosition
position: 69
reference: Q00740-1

22852456756293678tt75
type: Position
type: ExactPosition
position: 75
reference: Q00740-1

22852456756293678tt79
type: Position
type: ExactPosition
position: 79
reference: Q00740-1

22852456756293678tt91
type: Position
type: ExactPosition
position: 91
reference: Q00740-1

22852456756293678tt94
type: Position
type: ExactPosition
position: 94
reference: Q00740-1

22852456756293678tt100
type: Position
type: ExactPosition
position: 100
reference: Q00740-1

22852456756293678tt108
type: Position
type: ExactPosition
position: 108
reference: Q00740-1

22852456756293678tt112
type: Position
type: ExactPosition
position: 112
reference: Q00740-1

22852456756293678tt114
type: Position
type: ExactPosition
position: 114
reference: Q00740-1

22852456756293678tt121
type: Position
type: ExactPosition
position: 121
reference: Q00740-1

22852456756293678tt125
type: Position
type: ExactPosition
position: 125
reference: Q00740-1

22852456756293678tt128
type: Position
type: ExactPosition
position: 128
reference: Q00740-1

22852456756293678tt135
type: Position
type: ExactPosition
position: 135
reference: Q00740-1

22852456756293678tt138
type: Position
type: ExactPosition
position: 138
reference: Q00740-1

22852456756293678tt140
type: Position
type: ExactPosition
position: 140
reference: Q00740-1

22852456756293678tt150
type: Position
type: ExactPosition
position: 150
reference: Q00740-1

22852456756293678tt155
type: Position
type: ExactPosition
position: 155
reference: Q00740-1

22852456756293678tt157
type: Position
type: ExactPosition
position: 157
reference: Q00740-1

22852456756293678tt160
type: Position
type: ExactPosition
position: 160
reference: Q00740-1

22852456756293678tt162
type: Position
type: ExactPosition
position: 162
reference: Q00740-1

22852456756293678tt166
type: Position
type: ExactPosition
position: 166
reference: Q00740-1

22852456756293678tt169
type: Position
type: ExactPosition
position: 169
reference: Q00740-1

22852456756293678tt171
type: Position
type: ExactPosition
position: 171
reference: Q00740-1

22852456756293678tt186
type: Position
type: ExactPosition
position: 186
reference: Q00740-1

22852456756293678tt191
type: Position
type: ExactPosition
position: 191
reference: Q00740-1

22852456756293678tt200
type: Position
type: ExactPosition
position: 200
reference: Q00740-1

22852456756293678tt201
type: Position
type: ExactPosition
position: 201
reference: Q00740-1

22852456756293678tt211
type: Position
type: ExactPosition
position: 211
reference: Q00740-1

22852456756293678tt212
type: Position
type: ExactPosition
position: 212
reference: Q00740-1

22852456756293678tt214
type: Position
type: ExactPosition
position: 214
reference: Q00740-1

22852456756293678tt216
type: Position
type: ExactPosition
position: 216
reference: Q00740-1

22852456756293678tt218
type: Position
type: ExactPosition
position: 218
reference: Q00740-1

22852456756293678tt219
type: Position
type: ExactPosition
position: 219
reference: Q00740-1

22852456756293678tt223
type: Position
type: ExactPosition
position: 223
reference: Q00740-1

22852456756293678tt228
type: Position
type: ExactPosition
position: 228
reference: Q00740-1

22852456756293678tt246
type: Position
type: ExactPosition
position: 246
reference: Q00740-1

22852456756293678tt254
type: Position
type: ExactPosition
position: 254
reference: Q00740-1

22852456756293678tt256
type: Position
type: ExactPosition
position: 256
reference: Q00740-1

22852456756293678tt258
type: Position
type: ExactPosition
position: 258
reference: Q00740-1

22852456756293678tt260
type: Position
type: ExactPosition
position: 260
reference: Q00740-1

22852456756293678tt273
type: Position
type: ExactPosition
position: 273
reference: Q00740-1

22852456756293678tt286
type: Position
type: ExactPosition
position: 286
reference: Q00740-1

22852456756293678tt292
type: Position
type: ExactPosition
position: 292
reference: Q00740-1

22852456756293678tt294
type: Position
type: ExactPosition
position: 294
reference: Q00740-1

22852456756293678tt295
type: Position
type: ExactPosition
position: 295
reference: Q00740-1

22852456756293678tt307
type: Position
type: ExactPosition
position: 307
reference: Q00740-1

22852456756293678tt316
type: Position
type: ExactPosition
position: 316
reference: Q00740-1

22852456756293678tt321
type: Position
type: ExactPosition
position: 321
reference: Q00740-1

22852456756293678tt325
type: Position
type: ExactPosition
position: 325
reference: Q00740-1

22852456756293678tt331
type: Position
type: ExactPosition
position: 331
reference: Q00740-1

22852456756293678tt333
type: Position
type: ExactPosition
position: 333
reference: Q00740-1

22852456756293678tt335
type: Position
type: ExactPosition
position: 335
reference: Q00740-1

Q00740-1
type: Simple Sequence
modified: 1993-04-01
version: 1
mass: 36672
value: MNLQRFPRYPLTFGPTPIQPLARLSKHLGGKVHLYAKREDCNSGLAFGGNKTRKLEYLIPEALAQGCDTLVSIGGIQSNQTRQVAAVAAHLGMKCVLVQENWVNYSDAVYDRVGNIQMSRILGADVRLVPDGFDIGFRRSWEDALESVRAAGGKPYAIPAGCSDHPLGGLGFVGFAEEVRAQEAELGFKFDYVVVCSVTGSTQAGMVVGFAADGRADRVIGVDASAKPAQTREQITRIARQTAEKVGLERDIMRADVVLDERFAGPEYGLPNEGTLEAIRLCARTEGMLTDPVYEGKSMHGMIEMVRNGEFPEGSRVLYAHLGGVPALNGYSFIFRDG

http://purl.uniprot.org/uniprot/Q00740#attribution-EF49CFF7CB7DF66E7BB7BA4F7CE54F55
manual: true
evidence: ECO_0000255
source: MF_00807

http://purl.uniprot.org/uniprot/Q00740#attribution-0E16B74B1383D2C53B33CF8BBA9F02B8
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/1885510

P56653
type: Protein
reviewed: true
created: 1999-07-15
modified: 2023-09-13
version: 80
mnemonic: 4HBT_PSEUC
old mnemonic: 4HBT_PSESP
replaces: A5JTM7
citation: http://purl.uniprot.org/citations/1351742
citation: SIP1B3FB6BEB7CEBA2C
citation: http://purl.uniprot.org/citations/1610806
citation: http://purl.uniprot.org/citations/12220180
citation: http://purl.uniprot.org/citations/16962159
citation: http://purl.uniprot.org/citations/9837940
citation: http://purl.uniprot.org/citations/11997398
conflicting sequence: SIPC6EB3AC5AA69714D
recommended name: SIP4F1424A54E398A8E
enzyme: http://purl.uniprot.org/enzyme/3.1.2.23
organism: http://purl.uniprot.org/taxonomy/72586
annotation: SIP8AD48F5EAB20D83A
annotation: SIP7EC029B86F83FC6B
annotation: SIP365A9CBED74B94A1
annotation: SIP8C65E206B512DB76
annotation: SIPD187308FF169172E
annotation: SIPCBBACBE04C8801E7
annotation: SIP00E8E0121E3FE046
annotation: PRO_0000087760
annotation: SIPD65316FF484A22A0
annotation: SIP4A411906F1E3268D
annotation: SIP2C8EB2A6F8082768
annotation: SIPE376BAABB2E98989
annotation: SIPF59BCC1C6CE69682
annotation: SIPFAC0CEAE5AA03B9A
annotation: SIP10B38E7FCF2F514F
annotation: SIP82EAE4C53F0DCF84
annotation: SIPCF118F5728FB921A
annotation: SIPA7EF9EAE20498DAA
annotation: SIP9A0176507FDF4D8B
annotation: SIP3722DEE29EC1D16D
annotation: SIPAB818D637F2C130C
annotation: SIP9E917BCB46E3B4AC
annotation: SIP7BF377EEB9CF0F01
annotation: SIPB85F6FE2833CB11C
annotation: SIP2E2CD558358D0A8B
annotation: SIPD63573C3B3C8B4C9
annotation: SIP967EF7DD3DAF19E8
annotation: SIP7A12E0F23967A4B9
annotation: SIPF7BAC6DEEAD6F017
annotation: SIPA6A6F702FA30FCE2
annotation: SIPC359B66178CCA8B2
annotation: SIP3377C92C107E8F64
annotation: SIPCAA4ACB11954BC90
annotation: SIPD36C24D496365E55
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/2
classified with: http://purl.uniprot.org/keywords/378
classified with: GO_0018739
sequence: MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS

P56653-citation-1351742
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

SIP1B3FB6BEB7CEBA2C
type: Submission Citation
title: The nucleotide sequence upstream and downstream of 4-CBA-CoA ORFs in 9.5 kb Pseudomonas sp. strain CBS 3 chromosomal DNA.
author: Zhang W.
author: Dunaway-Mariano D.
date: 2007-04
submitted to: EMBL/GenBank/DDBJ

P56653-citation-SIP1B3FB6BEB7CEBA2C
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

P56653-citation-1610806
type: Citation Statement
scope: FUNCTION
scope: CATALYTIC ACTIVITY
scope: ACTIVITY REGULATION
scope: BIOPHYSICOCHEMICAL PROPERTIES
scope: PATHWAY
scope: SUBUNIT

P56653-citation-12220180
type: Citation Statement
scope: BIOPHYSICOCHEMICAL PROPERTIES
scope: MUTAGENESIS OF ASP-17; ARG-88; ARG-89; LYS-90; ARG-126 AND ARG-128

P56653-citation-16962159
type: Citation Statement
scope: BIOPHYSICOCHEMICAL PROPERTIES
scope: MUTAGENESIS OF ASP-32

P56653-citation-9837940
type: Citation Statement
scope: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS)
scope: SUBUNIT
scope: ACTIVE SITE

P56653-citation-11997398
type: Citation Statement
scope: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) OF WILD-TYPE IN COMPLEX WITH INHIBITOR AND MUTANT ASN-17 IN COMPLEX WITH SUBSTRATE
scope: SUBUNIT
scope: ACTIVE SITE

1BVQ
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.00
chain sequence mapping: PDB 1BVQ tt1tt141

PDB 1BVQ tt1tt141
chain: A=1-141

1LO7
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 1.50
chain sequence mapping: PDB 1LO7 tt1tt141

PDB 1LO7 tt1tt141
chain: A=1-141

1LO8
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 1.80
chain sequence mapping: PDB 1LO8 tt1tt141

PDB 1LO8 tt1tt141
chain: A=1-141

1LO9
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.80
chain sequence mapping: PDB 1LO9 tt1tt141

PDB 1LO9 tt1tt141
chain: A=1-141

DB01652
type: Resource
database: DrugBank
comment: 4-hydroxybenzoyl-CoA

DB04067
type: Resource
database: DrugBank
comment: 4-hydroxybenzyl coenzyme A

DB03613
type: Resource
database: DrugBank
comment: 4-hydroxyphenacyl coenzyme A

http://purl.uniprot.org/brenda/3.1.2.23
type: Resource
database: BRENDA
comment: 5085

cd00586
type: Resource
database: CDD
comment: 4HBT
signature sequence match: CDD cd00586 match 1

http://purl.uniprot.org/gene3d/3.10.129.10
type: Resource
database: Gene3D
comment: Hotdog Thioesterase
signature sequence match: Gene3D 3.10.129.10 match 1

IPR008272
type: Resource
database: InterPro
comment: HB-CoA_thioesterase_AS

IPR029069
type: Resource
database: InterPro
comment: HotDog_dom_sf

PF13279
type: Resource
database: Pfam
comment: 4HBT_2
signature sequence match: Pfam PF13279 match 1

SSF54637
type: Resource
database: SUPFAM
comment: Thioesterase/thiol ester dehydrase-isomerase
signature sequence match: SUPFAM SSF54637 match 1

PS01328
type: Resource
database: PROSITE
comment: 4HBCOA_THIOESTERASE
signature sequence match: PROSITE PS01328 match 1

SIP4F1424A54E398A8E
type: Structured Name
full name: 4-hydroxybenzoyl-CoA thioesterase
ec name: 3.1.2.23

SIP8AD48F5EAB20D83A
type: Function Annotation
comment: Hydrolyzes 4-hydroxybenzoate-CoA, and to a lesser extent benzoyl-CoA and 4-chlorobenzoate-CoA. Not active against aliphatic acyl-CoA thioesters, including palmitoyl-CoA, hexanoyl-CoA and acetyl-CoA.

SIP365A9CBED74B94A1
type: Activity Regulation Annotation
comment: Unaffected by EDTA, Mg(2+), Mn(2+), Fe(2+), Ca(2+), Co(2+) and Zn(2+).

SIP8C65E206B512DB76
type: Kinetics Annotation
measured affinity: 6 uM for 4-hydroxybenzoyl-CoA
measured affinity: 550 uM for 4-chlorobenzoyl-CoA
measured affinity: 200 uM for benzoyl-CoA
measured affinity: 270 uM for 4-hydroxybenzoyl-pantetheine
measured affinity: 56 uM for 4-methoxybenzoyl-CoA
measured affinity: 230 uM for 4-methylbenzoyl-CoA
measured affinity: 510 uM for benzoyl-CoA
measured affinity: 520 uM for 4-fluorobenzoyl-CoA
measured affinity: 300 uM for 4-trifluorobenzoyl-CoA

SIPCBBACBE04C8801E7
type: Subunit Annotation
comment: Homotetramer.

SIP00E8E0121E3FE046
type: Similarity Annotation
comment: Belongs to the 4-hydroxybenzoyl-CoA thioesterase family.

PRO_0000087760
type: Chain Annotation
comment: 4-hydroxybenzoyl-CoA thioesterase
mass: 16105
range: 22576505090814766tt1tt141

22576505090814766tt1
type: Position
type: ExactPosition
position: 1
reference: MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS

22576505090814766tt141
type: Position
type: ExactPosition
position: 141
reference: MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS

22576505090814766tt17
type: Position
type: ExactPosition
position: 17
reference: MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS

22576505090814766tt47
type: Position
type: ExactPosition
position: 47
reference: MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS

22576505090814766tt59
type: Position
type: ExactPosition
position: 59
reference: MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS

22576505090814766tt61
type: Position
type: ExactPosition
position: 61
reference: MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS

22576505090814766tt90
type: Position
type: ExactPosition
position: 90
reference: MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS

SIPF59BCC1C6CE69682
type: Mutagenesis Annotation
comment: Reduces catalytic activity. Little effect on substrate binding.
substitution: E
range: 22576505090814766tt17tt17

SIPFAC0CEAE5AA03B9A
type: Mutagenesis Annotation
comment: Drastically reduces catalytic activity. No effect on substrate binding.
substitution: N
range: 22576505090814766tt17tt17

SIP10B38E7FCF2F514F
type: Mutagenesis Annotation
comment: Drastically reduces catalytic activity.
substitution: S
range: 22576505090814766tt17tt17

SIP82EAE4C53F0DCF84
type: Mutagenesis Annotation
comment: Substrate turnover rate is decreased.
substitution: S
range: 22576505090814766tt32tt32

22576505090814766tt32
type: Position
type: ExactPosition
position: 32
reference: MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS

SIPCF118F5728FB921A
type: Mutagenesis Annotation
comment: No significant effect on catalytic activity or substrate binding.
substitution: A
range: 22576505090814766tt88tt88

22576505090814766tt88
type: Position
type: ExactPosition
position: 88
reference: MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS

SIPA7EF9EAE20498DAA
type: Mutagenesis Annotation
comment: No significant effect on catalytic activity or substrate binding.
substitution: L
range: 22576505090814766tt89tt89

22576505090814766tt89
type: Position
type: ExactPosition
position: 89
reference: MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS

SIP9A0176507FDF4D8B
type: Mutagenesis Annotation
comment: Decreases substrate binding affinity.
substitution: A
range: 22576505090814766tt90tt90

SIP3722DEE29EC1D16D
type: Mutagenesis Annotation
comment: No significant effect on catalytic activity or substrate binding.
substitution: L
range: 22576505090814766tt126tt126

22576505090814766tt126
type: Position
type: ExactPosition
position: 126
reference: MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS

SIPAB818D637F2C130C
type: Mutagenesis Annotation
comment: No significant effect on catalytic activity or substrate binding.
substitution: A
range: 22576505090814766tt128tt128

22576505090814766tt128
type: Position
type: ExactPosition
position: 128
reference: MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS

SIP9E917BCB46E3B4AC
type: Sequence Conflict Annotation
conflicting sequence: SIPC6EB3AC5AA69714D
substitution: L
range: 22576505090814766tt107tt107

22576505090814766tt107
type: Position
type: ExactPosition
position: 107
reference: MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS

22576505090814766tt4
type: Position
type: ExactPosition
position: 4
reference: MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS

22576505090814766tt10
type: Position
type: ExactPosition
position: 10
reference: MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS

22576505090814766tt13
type: Position
type: ExactPosition
position: 13
reference: MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS

22576505090814766tt15
type: Position
type: ExactPosition
position: 15
reference: MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS

22576505090814766tt20
type: Position
type: ExactPosition
position: 20
reference: MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS

22576505090814766tt22
type: Position
type: ExactPosition
position: 22
reference: MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS

22576505090814766tt25
type: Position
type: ExactPosition
position: 25
reference: MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS

22576505090814766tt41
type: Position
type: ExactPosition
position: 41
reference: MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS

22576505090814766tt54
type: Position
type: ExactPosition
position: 54
reference: MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS

22576505090814766tt56
type: Position
type: ExactPosition
position: 56
reference: MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS

22576505090814766tt58
type: Position
type: ExactPosition
position: 58
reference: MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS

22576505090814766tt68
type: Position
type: ExactPosition
position: 68
reference: MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS

22576505090814766tt77
type: Position
type: ExactPosition
position: 77
reference: MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS

22576505090814766tt87
type: Position
type: ExactPosition
position: 87
reference: MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS

22576505090814766tt101
type: Position
type: ExactPosition
position: 101
reference: MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS

22576505090814766tt123
type: Position
type: ExactPosition
position: 123
reference: MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS

22576505090814766tt130
type: Position
type: ExactPosition
position: 130
reference: MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS

22576505090814766tt134
type: Position
type: ExactPosition
position: 134
reference: MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS

22576505090814766tt140
type: Position
type: ExactPosition
position: 140
reference: MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS

substrate

MARSITMQQRIEFGDCDPAGIVWFPNYHRWLDAASRNYFIKCGLPPWRQTVVERGIVGTPIVSCNASFVCTASYDDVLTIETCIKEWRRKSFVQRHSVSRTTPGGDVQLVMRADEIRVFAMNDGERLRAIEVPADYIELCS
type: Simple Sequence
modified: 1999-07-15
version: 1
mass: 16105

http://purl.uniprot.org/uniprot/P56653#attribution-70E422B2EA93C07430AB73C11927325A
creator: UniProtKB
manual: true
evidence: ECO_0000314
source: http://purl.uniprot.org/citations/1610806

http://purl.uniprot.org/uniprot/P56653#attribution-B62A1D4D8A501CB3F9944BCA9C033012
manual: true
evidence: ECO_0000255
source: PRU10041

http://purl.uniprot.org/uniprot/P56653#attribution-1EAF11C815DDE117761B11D6CF421F56
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/11997398

http://purl.uniprot.org/uniprot/P56653#attribution-A48373DBD8F124B96BBF17443754931F
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/12220180

http://purl.uniprot.org/uniprot/P56653#attribution-9BCC8A110D803976C46301E9195B55F9
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/1610806

http://purl.uniprot.org/uniprot/P56653#attribution-2ADFB8C22303EA635D1658F5D983D45A
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/16962159

http://purl.uniprot.org/uniprot/P56653#attribution-67C8DBA34598E7B1388A993336EF03DC
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/9837940

http://purl.uniprot.org/uniprot/P56653#attribution-080ED3982FEC43F7C43A0689717F3D20
manual: true
evidence: ECO_0000305

P49172
type: Protein
reviewed: true
created: 1996-02-01
modified: 2023-09-13
version: 97
mnemonic: 4OT_PSEUF
old mnemonic: 4OT_PSESP
old mnemonic: DMPI_PSEPU
old mnemonic: DMPI_PSESP
citation: http://purl.uniprot.org/citations/1732207
citation: http://purl.uniprot.org/citations/8547259
recommended name: SIP31C078FAE2095590
alternative name: SIPC1BAFE50128FA6FD
enzyme: http://purl.uniprot.org/enzyme/5.3.2.6
organism: http://purl.uniprot.org/taxonomy/79676
encoded in: pVI150
annotation: SIP5BFFEB0CF919391B
annotation: SIPA679D84CAD49ABE4
annotation: SIPA4B0262F890C6075
annotation: SIP868F6DE8089330A7
annotation: SIP335DB7F849FAC066
annotation: SIPA5627E035CDF8732
annotation: PRO_0000209513
annotation: SIPAE9FECDEB7E39322
annotation: SIPBBAC3140E0D4B5F5
annotation: SIP4D6E48954202BD08
annotation: SIPFBC98EDC37034F1A
annotation: SIP31751C4DF68F600A
annotation: SIP22BFFF8048F895A4
annotation: SIPB61E28D15534C79D
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/2
classified with: http://purl.uniprot.org/keywords/58
classified with: http://purl.uniprot.org/keywords/413
classified with: http://purl.uniprot.org/keywords/614
classified with: GO_0016853
classified with: GO_0042203
sequence: MPIAQLYIIEGRTDEQKETLIRQVSEAMANSLDAPLERVRVLITEMPKNHFGIGGEPASKVRR

P49172-citation-1732207
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

P49172-citation-8547259
type: Citation Statement
scope: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS)

1OTF
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 1.90
chain sequence mapping: PDB 1OTF tt2tt63

PDB 1OTF tt2tt63
chain: A/B/C/D/E/F=2-63

http://purl.uniprot.org/brenda/5.3.2.6
type: Resource
database: BRENDA
comment: 5085

cd00491
type: Resource
database: CDD
comment: 4Oxalocrotonate_Tautomerase
signature sequence match: CDD cd00491 match 1

http://purl.uniprot.org/gene3d/3.30.429.10
type: Resource
database: Gene3D
comment: Macrophage Migration Inhibitory Factor
signature sequence match: Gene3D 3.30.429.10 match 1

IPR018191
type: Resource
database: InterPro
comment: 4-OT

IPR004370
type: Resource
database: InterPro
comment: 4-OT-like_dom

IPR014347
type: Resource
database: InterPro
comment: Tautomerase/MIF_sf

TIGR00013
type: Resource
database: NCBIfam
comment: taut
signature sequence match: NCBIfam TIGR00013 match 1

PTHR35530:SF1
type: Resource
database: PANTHER
comment: 2-HYDROXYMUCONATE TAUTOMERASE
signature sequence match: PANTHER PTHR35530:SF1 match 1

PTHR35530
type: Resource
database: PANTHER
comment: TAUTOMERASE-RELATED
signature sequence match: PANTHER PTHR35530 match 1

PF01361
type: Resource
database: Pfam
comment: Tautomerase
signature sequence match: Pfam PF01361 match 1

SSF55331
type: Resource
database: SUPFAM
comment: Tautomerase/MIF
signature sequence match: SUPFAM SSF55331 match 1

SIP31C078FAE2095590
type: Structured Name
full name: 2-hydroxymuconate tautomerase
ec name: 5.3.2.6

SIPC1BAFE50128FA6FD
type: Structured Name
full name: 4-oxalocrotonate tautomerase
short name: 4-OT

pVI150
type: Plasmid

dmpI
type: Gene

SIP5BFFEB0CF919391B
type: Function Annotation
comment: Catalyzes the ketonization of 2-hydroxymuconate stereoselectively to yield 2-oxo-3-hexenedioate.

SIP868F6DE8089330A7
type: Subunit Annotation
comment: Homohexamer.

SIP335DB7F849FAC066
type: Similarity Annotation
comment: Belongs to the 4-oxalocrotonate tautomerase family.

SIPA5627E035CDF8732
type: Initiator Methionine Annotation
comment: Removed
range: 22575418380333614tt1tt1

22575418380333614tt1
type: Position
type: ExactPosition
position: 1
reference: MPIAQLYIIEGRTDEQKETLIRQVSEAMANSLDAPLERVRVLITEMPKNHFGIGGEPASKVRR

PRO_0000209513
type: Chain Annotation
comment: 2-hydroxymuconate tautomerase
mass: 6974
range: 22575418380333614tt2tt63

22575418380333614tt2
type: Position
type: ExactPosition
position: 2
reference: MPIAQLYIIEGRTDEQKETLIRQVSEAMANSLDAPLERVRVLITEMPKNHFGIGGEPASKVRR

22575418380333614tt63
type: Position
type: ExactPosition
position: 63
reference: MPIAQLYIIEGRTDEQKETLIRQVSEAMANSLDAPLERVRVLITEMPKNHFGIGGEPASKVRR

SIPAE9FECDEB7E39322
type: Active Site Annotation
comment: Proton acceptor; via imino nitrogen
range: 22575418380333614tt2tt2

22575418380333614tt3
type: Position
type: ExactPosition
position: 3
reference: MPIAQLYIIEGRTDEQKETLIRQVSEAMANSLDAPLERVRVLITEMPKNHFGIGGEPASKVRR

22575418380333614tt10
type: Position
type: ExactPosition
position: 10
reference: MPIAQLYIIEGRTDEQKETLIRQVSEAMANSLDAPLERVRVLITEMPKNHFGIGGEPASKVRR

22575418380333614tt14
type: Position
type: ExactPosition
position: 14
reference: MPIAQLYIIEGRTDEQKETLIRQVSEAMANSLDAPLERVRVLITEMPKNHFGIGGEPASKVRR

22575418380333614tt32
type: Position
type: ExactPosition
position: 32
reference: MPIAQLYIIEGRTDEQKETLIRQVSEAMANSLDAPLERVRVLITEMPKNHFGIGGEPASKVRR

22575418380333614tt36
type: Position
type: ExactPosition
position: 36
reference: MPIAQLYIIEGRTDEQKETLIRQVSEAMANSLDAPLERVRVLITEMPKNHFGIGGEPASKVRR

22575418380333614tt38
type: Position
type: ExactPosition
position: 38
reference: MPIAQLYIIEGRTDEQKETLIRQVSEAMANSLDAPLERVRVLITEMPKNHFGIGGEPASKVRR

22575418380333614tt40
type: Position
type: ExactPosition
position: 40
reference: MPIAQLYIIEGRTDEQKETLIRQVSEAMANSLDAPLERVRVLITEMPKNHFGIGGEPASKVRR

22575418380333614tt46
type: Position
type: ExactPosition
position: 46
reference: MPIAQLYIIEGRTDEQKETLIRQVSEAMANSLDAPLERVRVLITEMPKNHFGIGGEPASKVRR

22575418380333614tt48
type: Position
type: ExactPosition
position: 48
reference: MPIAQLYIIEGRTDEQKETLIRQVSEAMANSLDAPLERVRVLITEMPKNHFGIGGEPASKVRR

22575418380333614tt50
type: Position
type: ExactPosition
position: 50
reference: MPIAQLYIIEGRTDEQKETLIRQVSEAMANSLDAPLERVRVLITEMPKNHFGIGGEPASKVRR

22575418380333614tt51
type: Position
type: ExactPosition
position: 51
reference: MPIAQLYIIEGRTDEQKETLIRQVSEAMANSLDAPLERVRVLITEMPKNHFGIGGEPASKVRR

22575418380333614tt53
type: Position
type: ExactPosition
position: 53
reference: MPIAQLYIIEGRTDEQKETLIRQVSEAMANSLDAPLERVRVLITEMPKNHFGIGGEPASKVRR

MPIAQLYIIEGRTDEQKETLIRQVSEAMANSLDAPLERVRVLITEMPKNHFGIGGEPASKVRR
type: Simple Sequence
modified: 2007-01-23
version: 2
mass: 7105

http://purl.uniprot.org/uniprot/P49172#attribution-609619A7DF3E53CE5E48EDEF8B8F9DD1
manual: true
evidence: ECO_0000250

http://purl.uniprot.org/uniprot/P49172#attribution-080ED3982FEC43F7C43A0689717F3D20
manual: true
evidence: ECO_0000305

Q52060
type: Protein
reviewed: true
created: 2011-02-08
modified: 2023-09-13
version: 102
mnemonic: ACDH_PSEUF
citation: http://purl.uniprot.org/citations/1732207
citation: http://purl.uniprot.org/citations/8419288
citation: http://purl.uniprot.org/citations/12764229
recommended name: SIPC3C24560DD252485
alternative name: SIP116DF1E75A719A69
enzyme: http://purl.uniprot.org/enzyme/1.2.1.10
organism: http://purl.uniprot.org/taxonomy/79676
encoded in: pVI150
annotation: SIP0515E0E80A6B39EB
annotation: SIPBD4C15B79DFAA551
annotation: SIPAF74C3C336094E34
annotation: SIP1D401DD7C8555AAA
annotation: SIPE79D3B1755FB87A8
annotation: SIP058ABD837794DB8F
annotation: SIPD958E4C428C4B4D9
annotation: PRO_0000404201
annotation: SIPF0C213D977B6D1AE
annotation: SIP73C2C0826E3680E3
annotation: SIPA64B65308E6E786D
annotation: SIPF6F086DE5A5172CA
annotation: SIPEE5BDBB326875295
annotation: SIP3E6FC8C376FBE3BD
annotation: SIP03805FC44CAA9BED
annotation: SIP6850177D3525EAA4
annotation: SIPC0AF7FBEA376C64B
annotation: SIP6B777DD732424B3B
annotation: SIPDDCB6268F0851E9B
annotation: SIP31709DCDE0D3A06F
annotation: SIP33E16B39E0A613DE
annotation: SIPC61139DDC156AED7
annotation: SIP48840AAEC49D9818
annotation: SIP2390A9F6180EF002
annotation: SIP9D11158043BAE0D8
annotation: SIPB2F2B626D4F9630A
annotation: SIP6EFC4BC4A2A3D3E5
annotation: SIPA11BF2F1BF5C856F
annotation: SIP62FD9B13EFE48A8C
annotation: SIP03AAA13BA2E43ADC
annotation: SIP7CF2A722485FF01D
annotation: SIPE110FDD49DCC43BF
annotation: SIP42A604BFD90A39B0
annotation: SIP918A66E9783FF804
annotation: SIPFE2AF5264E3F4A55
annotation: SIPA1168C2E352F5CCE
annotation: SIPC83113A0A78BE9F7
annotation: SIPEBED6A9513C9C001
annotation: SIPDAEEC58E55108BE0
annotation: SIP792E37F5592B2424
annotation: SIP3277BC31D8774356
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/2
classified with: http://purl.uniprot.org/keywords/58
classified with: http://purl.uniprot.org/keywords/903
classified with: http://purl.uniprot.org/keywords/520
classified with: http://purl.uniprot.org/keywords/560
classified with: http://purl.uniprot.org/keywords/614
classified with: GO_0008774
classified with: GO_0051287
classified with: GO_0050661
classified with: GO_0019439
classified with: GO_0019336
sequence: Q52060-1

Q52060-citation-1732207
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
scope: FUNCTION

CF600
type: Strain

Q52060-citation-8419288
type: Citation Statement
scope: PROTEIN SEQUENCE OF 1-6
scope: CATALYTIC ACTIVITY
scope: SUBSTRATE SPECIFICITY
scope: ACTIVITY REGULATION
scope: SUBUNIT
scope: INTERACTION WITH DMPG
scope: PH DEPENDENCE

Q52060-citation-12764229
type: Citation Statement
scope: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) IN COMPLEX WITH DMPG AND NAD
scope: SUBUNIT
scope: REACTION MECHANISM

1NVM
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 1.70
chain sequence mapping: PDB 1NVM tt1tt312
chain sequence mapping: PDB 1NVM tt1tt345

PDB 1NVM tt1tt312
chain: B/D/F/H=1-312

Q52060
type: Resource
database: IntAct
comment: 1

http://purl.uniprot.org/brenda/1.2.1.10
type: Resource
database: BRENDA
comment: 5085

http://purl.uniprot.org/gene3d/3.40.50.720
type: Resource
database: Gene3D
comment: NAD(P)-binding Rossmann-like Domain
signature sequence match: Gene3D 3.40.50.720 match 1
signature sequence match: Gene3D 3.40.50.720 match 2

MF_01657
type: Resource
database: HAMAP
comment: Ac_ald_DH_ac
signature sequence match: HAMAP MF 01657 match 1

IPR003361
type: Resource
database: InterPro
comment: Acetaldehyde_dehydrogenase

IPR015426
type: Resource
database: InterPro
comment: Acetylaldehyde_DH_C

IPR036291
type: Resource
database: InterPro
comment: NAD(P)-bd_dom_sf

IPR000534
type: Resource
database: InterPro
comment: Semialdehyde_DH_NAD-bd

TIGR03215
type: Resource
database: NCBIfam
comment: ac_ald_DH_ac
signature sequence match: NCBIfam TIGR03215 match 1

PF09290
type: Resource
database: Pfam
comment: AcetDehyd-dimer
signature sequence match: Pfam PF09290 match 1

PF01118
type: Resource
database: Pfam
comment: Semialdhyde_dh
signature sequence match: Pfam PF01118 match 1

PIRSF015689
type: Resource
database: PIRSF
comment: Actaldh_dh_actl
signature sequence match: PIRSF PIRSF015689 match 1

SM00859
type: Resource
database: SMART
comment: Semialdhyde_dh
signature sequence match: SMART SM00859 match 1

SSF55347
type: Resource
database: SUPFAM
comment: Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain
signature sequence match: SUPFAM SSF55347 match 1

SSF51735
type: Resource
database: SUPFAM
comment: NAD(P)-binding Rossmann-fold domains
signature sequence match: SUPFAM SSF51735 match 1

SIPC3C24560DD252485
type: Structured Name
full name: Acetaldehyde dehydrogenase
ec name: 1.2.1.10

SIP116DF1E75A719A69
type: Structured Name
full name: Acetaldehyde dehydrogenase [acetylating]

pVI150
type: Plasmid

dmpF
type: Gene

SIP0515E0E80A6B39EB
type: Function Annotation
comment: Catalyzes the conversion of acetaldehyde to acetyl-CoA, using NAD(+) and coenzyme A. Can also act on propanal and butanal to form propanoyl-CoA and butanoyl-CoA, respectively. Is the final enzyme in the meta-cleavage pathway for the degradation of aromatic compounds such as phenols, cresols and catechols. NADP(+) can replace NAD(+) but the rate of reaction is much slower.

SIPAF74C3C336094E34
type: Activity Regulation Annotation
comment: Is not activated by Mn(2+), Mg(2+), Ca(2+), Zn(2+) or Co(2+).

SIP1D401DD7C8555AAA
type: PH Dependence Annotation
comment: Activity increases gradually over the pH range 6.5-8.5.

SIP058ABD837794DB8F
type: Subunit Annotation
comment: Heterotetramer composed of two DmpG (aldolase) and two DmpF (dehydrogenase) subunits, which allows a direct channeling of acetaldehyde between the two active sites.

SIPD958E4C428C4B4D9
type: Similarity Annotation
comment: Belongs to the acetaldehyde dehydrogenase family.

PRO_0000404201
type: Chain Annotation
comment: Acetaldehyde dehydrogenase
mass: 32685
range: 22857962787057710tt1tt312

22857962787057710tt1
type: Position
type: ExactPosition
position: 1
reference: Q52060-1

22857962787057710tt312
type: Position
type: ExactPosition
position: 312
reference: Q52060-1

SIPF0C213D977B6D1AE
type: Active Site Annotation
comment: Acyl-thioester intermediate
range: 22857962787057710tt132tt132

22857962787057710tt132
type: Position
type: ExactPosition
position: 132
reference: Q52060-1

22857962787057710tt12
type: Position
type: ExactPosition
position: 12
reference: Q52060-1

22857962787057710tt15
type: Position
type: ExactPosition
position: 15
reference: Q52060-1

22857962787057710tt163
type: Position
type: ExactPosition
position: 163
reference: Q52060-1

22857962787057710tt171
type: Position
type: ExactPosition
position: 171
reference: Q52060-1

22857962787057710tt290
type: Position
type: ExactPosition
position: 290
reference: Q52060-1

22857962787057710tt5
type: Position
type: ExactPosition
position: 5
reference: Q52060-1

22857962787057710tt10
type: Position
type: ExactPosition
position: 10
reference: Q52060-1

22857962787057710tt14
type: Position
type: ExactPosition
position: 14
reference: Q52060-1

22857962787057710tt26
type: Position
type: ExactPosition
position: 26
reference: Q52060-1

22857962787057710tt28
type: Position
type: ExactPosition
position: 28
reference: Q52060-1

22857962787057710tt36
type: Position
type: ExactPosition
position: 36
reference: Q52060-1

22857962787057710tt43
type: Position
type: ExactPosition
position: 43
reference: Q52060-1

22857962787057710tt50
type: Position
type: ExactPosition
position: 50
reference: Q52060-1

22857962787057710tt55
type: Position
type: ExactPosition
position: 55
reference: Q52060-1

22857962787057710tt58
type: Position
type: ExactPosition
position: 58
reference: Q52060-1

22857962787057710tt59
type: Position
type: ExactPosition
position: 59
reference: Q52060-1

22857962787057710tt65
type: Position
type: ExactPosition
position: 65
reference: Q52060-1

22857962787057710tt67
type: Position
type: ExactPosition
position: 67
reference: Q52060-1

22857962787057710tt71
type: Position
type: ExactPosition
position: 71
reference: Q52060-1

22857962787057710tt72
type: Position
type: ExactPosition
position: 72
reference: Q52060-1

22857962787057710tt77
type: Position
type: ExactPosition
position: 77
reference: Q52060-1

22857962787057710tt81
type: Position
type: ExactPosition
position: 81
reference: Q52060-1

22857962787057710tt94
type: Position
type: ExactPosition
position: 94
reference: Q52060-1

22857962787057710tt99
type: Position
type: ExactPosition
position: 99
reference: Q52060-1

22857962787057710tt102
type: Position
type: ExactPosition
position: 102
reference: Q52060-1

22857962787057710tt114
type: Position
type: ExactPosition
position: 114
reference: Q52060-1

22857962787057710tt117
type: Position
type: ExactPosition
position: 117
reference: Q52060-1

22857962787057710tt118
type: Position
type: ExactPosition
position: 118
reference: Q52060-1

22857962787057710tt123
type: Position
type: ExactPosition
position: 123
reference: Q52060-1

22857962787057710tt125
type: Position
type: ExactPosition
position: 125
reference: Q52060-1

22857962787057710tt128
type: Position
type: ExactPosition
position: 128
reference: Q52060-1

22857962787057710tt145
type: Position
type: ExactPosition
position: 145
reference: Q52060-1

22857962787057710tt150
type: Position
type: ExactPosition
position: 150
reference: Q52060-1

22857962787057710tt160
type: Position
type: ExactPosition
position: 160
reference: Q52060-1

22857962787057710tt161
type: Position
type: ExactPosition
position: 161
reference: Q52060-1

22857962787057710tt166
type: Position
type: ExactPosition
position: 166
reference: Q52060-1

22857962787057710tt169
type: Position
type: ExactPosition
position: 169
reference: Q52060-1

22857962787057710tt172
type: Position
type: ExactPosition
position: 172
reference: Q52060-1

22857962787057710tt185
type: Position
type: ExactPosition
position: 185
reference: Q52060-1

22857962787057710tt190
type: Position
type: ExactPosition
position: 190
reference: Q52060-1

22857962787057710tt199
type: Position
type: ExactPosition
position: 199
reference: Q52060-1

22857962787057710tt207
type: Position
type: ExactPosition
position: 207
reference: Q52060-1

22857962787057710tt217
type: Position
type: ExactPosition
position: 217
reference: Q52060-1

22857962787057710tt220
type: Position
type: ExactPosition
position: 220
reference: Q52060-1

22857962787057710tt235
type: Position
type: ExactPosition
position: 235
reference: Q52060-1

22857962787057710tt241
type: Position
type: ExactPosition
position: 241
reference: Q52060-1

22857962787057710tt245
type: Position
type: ExactPosition
position: 245
reference: Q52060-1

22857962787057710tt248
type: Position
type: ExactPosition
position: 248
reference: Q52060-1

22857962787057710tt252
type: Position
type: ExactPosition
position: 252
reference: Q52060-1

22857962787057710tt258
type: Position
type: ExactPosition
position: 258
reference: Q52060-1

22857962787057710tt260
type: Position
type: ExactPosition
position: 260
reference: Q52060-1

22857962787057710tt261
type: Position
type: ExactPosition
position: 261
reference: Q52060-1

22857962787057710tt263
type: Position
type: ExactPosition
position: 263
reference: Q52060-1

22857962787057710tt264
type: Position
type: ExactPosition
position: 264
reference: Q52060-1

22857962787057710tt266
type: Position
type: ExactPosition
position: 266
reference: Q52060-1

22857962787057710tt268
type: Position
type: ExactPosition
position: 268
reference: Q52060-1

22857962787057710tt277
type: Position
type: ExactPosition
position: 277
reference: Q52060-1

22857962787057710tt282
type: Position
type: ExactPosition
position: 282
reference: Q52060-1

22857962787057710tt284
type: Position
type: ExactPosition
position: 284
reference: Q52060-1

22857962787057710tt289
type: Position
type: ExactPosition
position: 289
reference: Q52060-1

22857962787057710tt310
type: Position
type: ExactPosition
position: 310
reference: Q52060-1

Q52060-1
type: Simple Sequence
modified: 1996-11-01
version: 1
mass: 32685
value: MNQKLKVAIIGSGNIGTDLMIKVLRNAKYLEMGAMVGIDAASDGLARAQRMGVTTTYAGVEGLIKLPEFADIDFVFDATSASAHVQNEALLRQAKPGIRLIDLTPAAIGPYCVPVVNLEEHLGKLNVNMVTCGGQATIPMVAAVSRVAKVHYAEIVASISSKSAGPGTRANIDEFTETTSKAIEVIGGAAKGKAIIIMNPAEPPLIMRDTVYVLSAAADQAAVAASVAEMVQAVQAYVPGYRLKQQVQFDVIPESAPLNIPGLGRFSGLKTSVFLEVEGAAHYLPAYAGNLDIMTSAALATAERMAQSMLNA

http://purl.uniprot.org/uniprot/Q52060#attribution-44F726B6481B3054005E8DE690D0A2B6
creator: UniProtKB
manual: true
evidence: ECO_0000314
source: http://purl.uniprot.org/citations/8419288

http://purl.uniprot.org/uniprot/Q52060#attribution-A474DB4F1AC35E93635077AA1A324865
creator: UniProtKB
manual: true
evidence: ECO_0000314
source: http://purl.uniprot.org/citations/12764229

http://purl.uniprot.org/uniprot/Q52060#attribution-F248E7B692F3F3A167EED240EFCEF57F
manual: true
evidence: ECO_0000255
source: MF_01657

http://purl.uniprot.org/uniprot/Q52060#attribution-2896F89442AE0BAB4AA8721FBD8C1C43
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/12764229

http://purl.uniprot.org/uniprot/Q52060#attribution-5BE3D9FC39CCB10D8A4B1CB4C5034062
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/1732207

http://purl.uniprot.org/uniprot/Q52060#attribution-09B7B99FA8656CCA3E840206F13D3A3B
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/8419288

http://purl.uniprot.org/uniprot/Q52060#attribution-080ED3982FEC43F7C43A0689717F3D20
manual: true
evidence: ECO_0000305

Q06365
type: Protein
reviewed: true
created: 1998-12-15
modified: 2023-05-03
version: 71
mnemonic: ALYP_PSESO
citation: http://purl.uniprot.org/citations/8336113
recommended name: SIP6312F6455255EC4D
alternative name: SIP7C24EF6D04A27264
alternative name: SIPFE005BE86183C4F2
enzyme: http://purl.uniprot.org/enzyme/4.2.2.3
organism: http://purl.uniprot.org/taxonomy/86038
annotation: SIP0F9828FDDF10AC41
annotation: SIPAFBEAC7865D5EDE6
annotation: SIP1DA8836C6EB06127
annotation: PRO_0000024924
existence: Inferred from Homology Existence
classified with: http://purl.uniprot.org/keywords/456
classified with: http://purl.uniprot.org/keywords/732
classified with: GO_0045135
sequence: Q06365-1

Q06365-citation-8336113
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

PL6
type: Resource
database: CAZy
comment: Polysaccharide Lyase Family 6

cd14251
type: Resource
database: CDD
comment: PL-6
signature sequence match: CDD cd14251 match 1

http://purl.uniprot.org/gene3d/2.160.20.10
type: Resource
database: Gene3D
comment: Single-stranded right-handed beta-helix, Pectin lyase-like
signature sequence match: Gene3D 2.160.20.10 match 1

IPR012334
type: Resource
database: InterPro
comment: Pectin_lyas_fold

IPR011050
type: Resource
database: InterPro
comment: Pectin_lyase_fold/virulence

IPR039513
type: Resource
database: InterPro
comment: PL-6

PF14592
type: Resource
database: Pfam
comment: Chondroitinas_B
signature sequence match: Pfam PF14592 match 1

SSF51126
type: Resource
database: SUPFAM
comment: Pectin lyase-like
signature sequence match: SUPFAM SSF51126 match 1

SIP6312F6455255EC4D
type: Structured Name
full name: Alginate lyase
ec name: 4.2.2.3

SIP7C24EF6D04A27264
type: Structured Name
full name: Poly(beta-D-mannuronate) lyase

SIPFE005BE86183C4F2
type: Structured Name
full name: Poly(mana) alginate lyase

aly
type: Gene
alternative label: alyP

SIPAFBEAC7865D5EDE6
type: Similarity Annotation
comment: Belongs to the polysaccharide lyase 6 family.

22852482459120942tt1
type: Position
type: ExactPosition
position: 1
reference: Q06365-1

PRO_0000024924
type: Chain Annotation
comment: Alginate lyase
mass: 44512
range: 22852482459120942nbtt398

22852482459120942tt398
type: Position
type: ExactPosition
position: 398
reference: Q06365-1

Q06365-1
type: Simple Sequence
modified: 1996-11-01
version: 1
precursor: true
mass: 44512
value: MPGDKVIMKSGEWKSQFIHFKGKGTAEKPITLTAETKGSVLLTGNSNLKIDGEWLVVDGLSFKNGFSLKDDVVVFTKTTTNSRLTNTSIENYNPVDKTLDYKWVSLYGHHNRVDHCSITGKNHQGTTLVVWLDDKPNYHQIDHNYFGPRPELGANGGETIRIGTSAFSMNDSYRTVQNNIFDKCDGEVEIISIKSGFNKILNNLFYECAGTVTFRHGNNSEVSNNYFIANNVTNSGGVRIIGENQKVYGNYLYKVAGRTLRSAISVMNAYEKPALNDYWQVKNADIQNNIIVGAREAFVLGSGKDNDRTLAPDGVNISNNYIINPTTLLVTQDEPKNLKMQNNQVEGASIVTGFVKMGNDLQMSDGIWQKKTEIKKPFWLATAIGPEWKKDHRSFIFK

http://purl.uniprot.org/uniprot/Q06365#attribution-DC036A124EC8E88DF072B29F6D9BB9FE
manual: true
evidence: ECO_0000255

http://purl.uniprot.org/uniprot/Q06365#attribution-080ED3982FEC43F7C43A0689717F3D20
manual: true
evidence: ECO_0000305

P0A2Y4
type: Protein
reviewed: true
created: 2005-03-15
modified: 2023-09-13
version: 72
mnemonic: AROA_PSES2
replaces: P56952
citation: SIP35597ACFE246013D
recommended name: SIP9B7B8EEDD6AC7691
alternative name: SIP7BCBDA8035E4F966
enzyme: http://purl.uniprot.org/enzyme/2.5.1.19
organism: http://purl.uniprot.org/taxonomy/308
annotation: SIP5ED583DB6730D48C
annotation: SIP24846A69D61BE2EB
annotation: SIPD2122A9173D4BA3A
annotation: SIP613165B98C268932
annotation: SIPD854721C1B6F9840
annotation: SIP96A3AB72C8AA8395
annotation: SIPDBE294CD578ED56C
annotation: PRO_0000088280
annotation: SIP6E95F9058123A812
annotation: SIP64E8ED84288A7333
annotation: SIPA2140D079E8EAD1D
annotation: SIP0A2DE141DFBA38C9
annotation: SIP337F931FEE0963A3
annotation: SIP1B01616C5EC17239
annotation: SIP08E808DE4CC63E69
annotation: SIP5CDC350B74DB7FDB
annotation: SIPBE8410FB3EFAF6E4
annotation: SIPA8B3CA58814DC386
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/57
classified with: http://purl.uniprot.org/keywords/963
classified with: http://purl.uniprot.org/keywords/903
classified with: http://purl.uniprot.org/keywords/359
classified with: http://purl.uniprot.org/keywords/808
classified with: GO_0005737
classified with: GO_0003866
classified with: GO_0008652
classified with: GO_0009073
classified with: GO_0009423
classified with: GO_0009635
sequence: P0A2Y4-1

SIP35597ACFE246013D
type: Patent Citation
title: Glyphosate-tolerant 5-enolpyruvylshikimate-3-phosphate synthases.
author: Barry G.F.
author: Kishore G.M.
author: Padgette S.R.
author: Stallings W.C.
date: 1997-05-27

P0A2Y4-citation-SIP35597ACFE246013D
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
scope: PROTEIN SEQUENCE OF 2-16

cd01556
type: Resource
database: CDD
comment: EPSP_synthase
signature sequence match: CDD cd01556 match 1

http://purl.uniprot.org/gene3d/3.65.10.10
type: Resource
database: Gene3D
comment: Enolpyruvate transferase domain
signature sequence match: Gene3D 3.65.10.10 match 1
signature sequence match: Gene3D 3.65.10.10 match 2

MF_00210
type: Resource
database: HAMAP
comment: EPSP_synth
signature sequence match: HAMAP MF 00210 match 1

IPR001986
type: Resource
database: InterPro
comment: Enolpyruvate_Tfrase_dom

IPR036968
type: Resource
database: InterPro
comment: Enolpyruvate_Tfrase_sf

IPR006264
type: Resource
database: InterPro
comment: EPSP_synthase

IPR023193
type: Resource
database: InterPro
comment: EPSP_synthase_CS

IPR013792
type: Resource
database: InterPro
comment: RNA3'P_cycl/enolpyr_Trfase_a/b

TIGR01356
type: Resource
database: NCBIfam
comment: aroA
signature sequence match: NCBIfam TIGR01356 match 1

PTHR21090
type: Resource
database: PANTHER
comment: AROM/DEHYDROQUINATE SYNTHASE
signature sequence match: PANTHER PTHR21090 match 1

PTHR21090:SF5
type: Resource
database: PANTHER
comment: PENTAFUNCTIONAL AROM POLYPEPTIDE
signature sequence match: PANTHER PTHR21090:SF5 match 1

PF00275
type: Resource
database: Pfam
comment: EPSP_synthase
signature sequence match: Pfam PF00275 match 1

PIRSF000505
type: Resource
database: PIRSF
comment: EPSPS
signature sequence match: PIRSF PIRSF000505 match 1

SSF55205
type: Resource
database: SUPFAM
comment: EPT/RTPC-like
signature sequence match: SUPFAM SSF55205 match 1

PS00104
type: Resource
database: PROSITE
comment: EPSP_SYNTHASE_1
signature sequence match: PROSITE PS00104 match 1

PS00885
type: Resource
database: PROSITE
comment: EPSP_SYNTHASE_2
signature sequence match: PROSITE PS00885 match 1

SIP9B7B8EEDD6AC7691
type: Structured Name
full name: 3-phosphoshikimate 1-carboxyvinyltransferase
ec name: 2.5.1.19

SIP7BCBDA8035E4F966
type: Structured Name
full name: 5-enolpyruvylshikimate-3-phosphate synthase
short name: EPSP synthase
short name: EPSPS

aroA
type: Gene

SIP5ED583DB6730D48C
type: Function Annotation
comment: Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate.

SIP613165B98C268932
type: Subunit Annotation
comment: Monomer.

SIP96A3AB72C8AA8395
type: Annotation
comment: Resistant to the antibiotic glyphosate.

SIPDBE294CD578ED56C
type: Similarity Annotation
comment: Belongs to the EPSP synthase family.

PRO_0000088280
type: Chain Annotation
comment: 3-phosphoshikimate 1-carboxyvinyltransferase
mass: 47297
range: 22571054712566830tt1tt449

22571054712566830tt1
type: Position
type: ExactPosition
position: 1
reference: P0A2Y4-1

22571054712566830tt449
type: Position
type: ExactPosition
position: 449
reference: P0A2Y4-1

SIP6E95F9058123A812
type: Region Annotation
comment: Disordered
range: 22571054712566830tt1tt23

22571054712566830tt23
type: Position
type: ExactPosition
position: 23
reference: P0A2Y4-1

SIP64E8ED84288A7333
type: Region Annotation
comment: Phosphoenolpyruvate
range: 22571054712566830tt98tt101

22571054712566830tt98
type: Position
type: ExactPosition
position: 98
reference: P0A2Y4-1

22571054712566830tt101
type: Position
type: ExactPosition
position: 101
reference: P0A2Y4-1

SIPA2140D079E8EAD1D
type: Active Site Annotation
comment: Proton acceptor
range: 22571054712566830tt326tt326

22571054712566830tt326
type: Position
type: ExactPosition
position: 326
reference: P0A2Y4-1

SIP0A2DE141DFBA38C9
type: Active Site Annotation
comment: Proton donor
range: 22571054712566830tt354tt354

22571054712566830tt354
type: Position
type: ExactPosition
position: 354
reference: P0A2Y4-1

22571054712566830tt28
type: Position
type: ExactPosition
position: 28
reference: P0A2Y4-1

22571054712566830tt29
type: Position
type: ExactPosition
position: 29
reference: P0A2Y4-1

22571054712566830tt33
type: Position
type: ExactPosition
position: 33
reference: P0A2Y4-1

22571054712566830tt128
type: Position
type: ExactPosition
position: 128
reference: P0A2Y4-1

22571054712566830tt353
type: Position
type: ExactPosition
position: 353
reference: P0A2Y4-1

22571054712566830tt357
type: Position
type: ExactPosition
position: 357
reference: P0A2Y4-1

22571054712566830tt402
type: Position
type: ExactPosition
position: 402
reference: P0A2Y4-1

P0A2Y4-1
type: Simple Sequence
modified: 2005-03-15
version: 1
mass: 47297
value: MSHSASPKPATARRSEALTGEIRIPGDKSISHRSFMFGGLASGETRITGLLEGEDVINTGRAMQAMGAKIRKEGDVWIINGVGNGCLLQPEAALDFGNAGTGARLTMGLVGTYDMKTSFIGDASLSKRPMGRVLNPLREMGVQVEAADGDRMPLTLIGPKTANPITYRVPMASAQVKSAVLLAGLNTPGVTTVIEPVMTRDHTEKMLQGFGADLTVETDKDGVRHIRITGQGKLVGQTIDVPGDPSSTAFPLVAALLVEGSDVTIRNVLMNPTRTGLILTLQEMGADIEVLNARLAGGEDVADLRVRASKLKGVVVPPERAPSMIDEYPVLAIAASFAEGETVMDGLDELRVKESDRLAAVARGLEANGVDCTEGEMSLTVRGRPDGKGLGGGTVATHLDHRIAMSFLVMGLAAEKPVTVDDSNMIATSFPEFMDMMPGLGAKIELSIL

http://purl.uniprot.org/uniprot/P0A2Y4#attribution-0A561F4F067256E9CA6406750D5E4A5F
manual: true
evidence: ECO_0000255
source: MF_00210

http://purl.uniprot.org/uniprot/P0A2Y4#attribution-080ED3982FEC43F7C43A0689717F3D20
manual: true
evidence: ECO_0000305

P10182
type: Protein
reviewed: true
created: 1989-07-01
modified: 2023-09-13
version: 102
mnemonic: ASPQ_PSES7
old mnemonic: ASPQ_PSESP
citation: http://purl.uniprot.org/citations/8068664
citation: http://purl.uniprot.org/citations/619999
citation: http://purl.uniprot.org/citations/9020792
citation: http://purl.uniprot.org/citations/10684596
conflicting sequence: SIPC8BC812CE7E9D4B4
recommended name: SIPD87756D7FEBCFCFD
alternative name: SIP753887D11BCB0194
alternative name: SIP2CCF2CFF0992EA89
enzyme: http://purl.uniprot.org/enzyme/3.5.1.38
organism: http://purl.uniprot.org/taxonomy/313
annotation: SIPE8F6A7881E874169
annotation: SIPA74DBC5428ABAB2D
annotation: SIP53D6978A1B0ED1DE
annotation: SIPC45A5B8CFC10BE43
annotation: SIPF41BA78C1170023A
annotation: PRO_0000171089
annotation: SIPB65FD4840891480D
annotation: SIP01B9D40BB725A062
annotation: SIP4ADB3D389944403C
annotation: SIP0F14209CFC3A5526
annotation: SIP16B8DB373625623F
annotation: SIPE26164CA2452FD80
annotation: SIP21100A7DC69D6870
annotation: SIPA438626B6144E533
annotation: SIPA7DD44088150A537
annotation: SIP1C25B4A12D48975B
annotation: SIP2A5719BCE8512E26
annotation: SIP5A3688C84BAC259C
annotation: SIPE0387D47B484ED36
annotation: SIPD3DFB2E7484B4B3A
annotation: SIP38DF699E132F5C74
annotation: SIP69323C6D698030C1
annotation: SIPD415F3328CE7C39D
annotation: SIP0E2978DBBCA31ECF
annotation: SIPD1BF11C9C90853F4
annotation: SIP4D47111D9F9B46D6
annotation: SIP9DB6482FC3B4ACE0
annotation: SIPA9A3B7D96748433C
annotation: SIP4DAEF5E6FD9ACA47
annotation: SIPB16186B5C70BB5F2
annotation: SIPF94F3C42274E40A3
annotation: SIP4EAF1AD6AFF48347
annotation: SIP34E9AC92D3C94A73
annotation: SIP643A6E97B0AAC4C9
annotation: SIPDA1D3CC6311900BF
annotation: SIPD338AC0E970B8374
annotation: SIP9C29B5CF6F284B3A
annotation: SIP47E7B11663B43D23
annotation: SIP0109FB2D7AB3099A
annotation: SIP1B3FDB572C67F4AF
annotation: SIPD3953690E0B87EF1
annotation: SIPE847BFA255507A1C
annotation: SIP39E699654643E7CE
annotation: SIP71726FDDE3DFB30D
annotation: SIP82B0EE58F4A38362
annotation: SIPB209B8797EADC3F4
annotation: SIPFD95ACA79001C8CF
annotation: SIP9C0E073CB4E7792F
annotation: SIP34115368E348747C
annotation: SIPED3BC7643CD2CF82
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/2
classified with: http://purl.uniprot.org/keywords/903
classified with: http://purl.uniprot.org/keywords/378
classified with: http://purl.uniprot.org/keywords/574
classified with: GO_0042597
classified with: GO_0004067
classified with: GO_0050417
classified with: GO_0004359
classified with: GO_0006528
sequence: P10182-1

P10182-citation-8068664
type: Citation Statement
scope: PROTEIN SEQUENCE
scope: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS)

P10182-citation-619999
type: Citation Statement
scope: PROTEIN SEQUENCE OF 1-26

P10182-citation-9020792
type: Citation Statement
scope: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS)

P10182-citation-10684596
type: Citation Statement
scope: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 8-337 IN COMPLEXES WITH 6-DIAZO-5-OXO-L-NORLEUCINE AND 5-DIAZO-4-OXO-L-NORVALINE
scope: ACTIVE SITE
scope: SUBUNIT

1DJO
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.00
chain sequence mapping: PDB 1DJO tt8tt337

PDB 1DJO tt8tt337
chain: A/B=8-337

1DJP
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 1.90
chain sequence mapping: PDB 1DJP tt8tt337

PDB 1DJP tt8tt337
chain: A/B=8-337

3PGA
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.00
chain sequence mapping: PDB 3PGA tt8tt337

PDB 3PGA tt8tt337
chain: 1/2/3/4=8-337

4PGA
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 1.70
chain sequence mapping: PDB 4PGA tt1tt337

PDB 4PGA tt1tt337
chain: A/B=1-337

http://purl.uniprot.org/brenda/3.5.1.38
type: Resource
database: BRENDA
comment: 5085

cd00411
type: Resource
database: CDD
comment: L-asparaginase_like
signature sequence match: CDD cd00411 match 1

http://purl.uniprot.org/gene3d/3.40.50.1170
type: Resource
database: Gene3D
comment: L-asparaginase, N-terminal domain
signature sequence match: Gene3D 3.40.50.1170 match 1

IPR004550
type: Resource
database: InterPro
comment: AsnASE_II

IPR036152
type: Resource
database: InterPro
comment: Asp/glu_Ase-like_sf

IPR006034
type: Resource
database: InterPro
comment: Asparaginase/glutaminase-like

IPR020827
type: Resource
database: InterPro
comment: Asparaginase/glutaminase_AS1

IPR027475
type: Resource
database: InterPro
comment: Asparaginase/glutaminase_AS2

IPR040919
type: Resource
database: InterPro
comment: Asparaginase_C

IPR027473
type: Resource
database: InterPro
comment: L-asparaginase_C

IPR027474
type: Resource
database: InterPro
comment: L-asparaginase_N

IPR037152
type: Resource
database: InterPro
comment: L-asparaginase_N_sf

TIGR00520
type: Resource
database: NCBIfam
comment: asnASE_II
signature sequence match: NCBIfam TIGR00520 match 1

PTHR11707:SF28
type: Resource
database: PANTHER
comment: 60 KDA LYSOPHOSPHOLIPASE
signature sequence match: PANTHER PTHR11707:SF28 match 1

PTHR11707
type: Resource
database: PANTHER
comment: L-ASPARAGINASE
signature sequence match: PANTHER PTHR11707 match 1

PF00710
type: Resource
database: Pfam
comment: Asparaginase
signature sequence match: Pfam PF00710 match 1

PF17763
type: Resource
database: Pfam
comment: Asparaginase_C
signature sequence match: Pfam PF17763 match 1

PIRSF001220
type: Resource
database: PIRSF
comment: L-ASNase_gatD
signature sequence match: PIRSF PIRSF001220 match 1

PIRSF500176
type: Resource
database: PIRSF
comment: L_ASNase
signature sequence match: PIRSF PIRSF500176 match 1

PR00139
type: Resource
database: PRINTS
comment: ASNGLNASE

SM00870
type: Resource
database: SMART
comment: Asparaginase
signature sequence match: SMART SM00870 match 1

SSF53774
type: Resource
database: SUPFAM
comment: Glutaminase/Asparaginase
signature sequence match: SUPFAM SSF53774 match 1

PS00144
type: Resource
database: PROSITE
comment: ASN_GLN_ASE_1
signature sequence match: PROSITE PS00144 match 1

PS00917
type: Resource
database: PROSITE
comment: ASN_GLN_ASE_2
signature sequence match: PROSITE PS00917 match 1

PS51732
type: Resource
database: PROSITE
comment: ASN_GLN_ASE_3
signature sequence match: PROSITE PS51732 match 1

SIPC8BC812CE7E9D4B4
type: External Sequence
comment: AA sequence
citation: http://purl.uniprot.org/citations/619999

SIPD87756D7FEBCFCFD
type: Structured Name
full name: Glutaminase-asparaginase
ec name: 3.5.1.38

SIP753887D11BCB0194
type: Structured Name
full name: L-ASNase/L-GLNase

SIP2CCF2CFF0992EA89
type: Structured Name
full name: L-asparagine/L-glutamine amidohydrolase
short name: PGA

ansB
type: Gene

SIP53D6978A1B0ED1DE
type: Subunit Annotation
comment: Homotetramer.

SIPF41BA78C1170023A
type: Similarity Annotation
comment: Belongs to the asparaginase 1 family.

PRO_0000171089
type: Chain Annotation
comment: Glutaminase-asparaginase
mass: 36200
range: 22572081190810158tt1tt337

22572081190810158tt1
type: Position
type: ExactPosition
position: 1
reference: P10182-1

22572081190810158tt337
type: Position
type: ExactPosition
position: 337
reference: P10182-1

SIPB65FD4840891480D
type: Domain Extent Annotation
comment: Asparaginase/glutaminase
range: 22572081190810158tt10tt337

22572081190810158tt10
type: Position
type: ExactPosition
position: 10
reference: P10182-1

SIP01B9D40BB725A062
type: Active Site Annotation
comment: Acyl-ester intermediate
range: 22572081190810158tt20tt20

22572081190810158tt20
type: Position
type: ExactPosition
position: 20
reference: P10182-1

22572081190810158tt67
type: Position
type: ExactPosition
position: 67
reference: P10182-1

22572081190810158tt100
type: Position
type: ExactPosition
position: 100
reference: P10182-1

22572081190810158tt101
type: Position
type: ExactPosition
position: 101
reference: P10182-1

SIP16B8DB373625623F
type: Sequence Conflict Annotation
conflicting sequence: SIPC8BC812CE7E9D4B4
substitution: H
range: 22572081190810158tt7tt7

22572081190810158tt7
type: Position
type: ExactPosition
position: 7
reference: P10182-1

SIPE26164CA2452FD80
type: Sequence Conflict Annotation
conflicting sequence: SIPC8BC812CE7E9D4B4
substitution: R
range: 22572081190810158tt12tt12

22572081190810158tt12
type: Position
type: ExactPosition
position: 12
reference: P10182-1

22572081190810158tt11
type: Position
type: ExactPosition
position: 11
reference: P10182-1

22572081190810158tt19
type: Position
type: ExactPosition
position: 19
reference: P10182-1

22572081190810158tt22
type: Position
type: ExactPosition
position: 22
reference: P10182-1

22572081190810158tt26
type: Position
type: ExactPosition
position: 26
reference: P10182-1

22572081190810158tt29
type: Position
type: ExactPosition
position: 29
reference: P10182-1

22572081190810158tt31
type: Position
type: ExactPosition
position: 31
reference: P10182-1

22572081190810158tt37
type: Position
type: ExactPosition
position: 37
reference: P10182-1

22572081190810158tt41
type: Position
type: ExactPosition
position: 41
reference: P10182-1

22572081190810158tt46
type: Position
type: ExactPosition
position: 46
reference: P10182-1

22572081190810158tt51
type: Position
type: ExactPosition
position: 51
reference: P10182-1

22572081190810158tt54
type: Position
type: ExactPosition
position: 54
reference: P10182-1

22572081190810158tt56
type: Position
type: ExactPosition
position: 56
reference: P10182-1

22572081190810158tt65
type: Position
type: ExactPosition
position: 65
reference: P10182-1

22572081190810158tt69
type: Position
type: ExactPosition
position: 69
reference: P10182-1

22572081190810158tt72
type: Position
type: ExactPosition
position: 72
reference: P10182-1

22572081190810158tt87
type: Position
type: ExactPosition
position: 87
reference: P10182-1

22572081190810158tt92
type: Position
type: ExactPosition
position: 92
reference: P10182-1

22572081190810158tt97
type: Position
type: ExactPosition
position: 97
reference: P10182-1

22572081190810158tt102
type: Position
type: ExactPosition
position: 102
reference: P10182-1

22572081190810158tt103
type: Position
type: ExactPosition
position: 103
reference: P10182-1

22572081190810158tt113
type: Position
type: ExactPosition
position: 113
reference: P10182-1

22572081190810158tt120
type: Position
type: ExactPosition
position: 120
reference: P10182-1

22572081190810158tt123
type: Position
type: ExactPosition
position: 123
reference: P10182-1

22572081190810158tt136
type: Position
type: ExactPosition
position: 136
reference: P10182-1

22572081190810158tt147
type: Position
type: ExactPosition
position: 147
reference: P10182-1

22572081190810158tt150
type: Position
type: ExactPosition
position: 150
reference: P10182-1

22572081190810158tt152
type: Position
type: ExactPosition
position: 152
reference: P10182-1

22572081190810158tt157
type: Position
type: ExactPosition
position: 157
reference: P10182-1

22572081190810158tt159
type: Position
type: ExactPosition
position: 159
reference: P10182-1

22572081190810158tt164
type: Position
type: ExactPosition
position: 164
reference: P10182-1

22572081190810158tt167
type: Position
type: ExactPosition
position: 167
reference: P10182-1

22572081190810158tt168
type: Position
type: ExactPosition
position: 168
reference: P10182-1

22572081190810158tt170
type: Position
type: ExactPosition
position: 170
reference: P10182-1

22572081190810158tt171
type: Position
type: ExactPosition
position: 171
reference: P10182-1

22572081190810158tt173
type: Position
type: ExactPosition
position: 173
reference: P10182-1

22572081190810158tt175
type: Position
type: ExactPosition
position: 175
reference: P10182-1

22572081190810158tt178
type: Position
type: ExactPosition
position: 178
reference: P10182-1

22572081190810158tt182
type: Position
type: ExactPosition
position: 182
reference: P10182-1

22572081190810158tt184
type: Position
type: ExactPosition
position: 184
reference: P10182-1

22572081190810158tt189
type: Position
type: ExactPosition
position: 189
reference: P10182-1

22572081190810158tt193
type: Position
type: ExactPosition
position: 193
reference: P10182-1

22572081190810158tt196
type: Position
type: ExactPosition
position: 196
reference: P10182-1

22572081190810158tt199
type: Position
type: ExactPosition
position: 199
reference: P10182-1

22572081190810158tt201
type: Position
type: ExactPosition
position: 201
reference: P10182-1

22572081190810158tt203
type: Position
type: ExactPosition
position: 203
reference: P10182-1

22572081190810158tt208
type: Position
type: ExactPosition
position: 208
reference: P10182-1

22572081190810158tt210
type: Position
type: ExactPosition
position: 210
reference: P10182-1

22572081190810158tt215
type: Position
type: ExactPosition
position: 215
reference: P10182-1

22572081190810158tt217
type: Position
type: ExactPosition
position: 217
reference: P10182-1

22572081190810158tt224
type: Position
type: ExactPosition
position: 224
reference: P10182-1

22572081190810158tt228
type: Position
type: ExactPosition
position: 228
reference: P10182-1

22572081190810158tt236
type: Position
type: ExactPosition
position: 236
reference: P10182-1

22572081190810158tt243
type: Position
type: ExactPosition
position: 243
reference: P10182-1

22572081190810158tt247
type: Position
type: ExactPosition
position: 247
reference: P10182-1

22572081190810158tt254
type: Position
type: ExactPosition
position: 254
reference: P10182-1

22572081190810158tt255
type: Position
type: ExactPosition
position: 255
reference: P10182-1

22572081190810158tt257
type: Position
type: ExactPosition
position: 257
reference: P10182-1

22572081190810158tt261
type: Position
type: ExactPosition
position: 261
reference: P10182-1

22572081190810158tt263
type: Position
type: ExactPosition
position: 263
reference: P10182-1

22572081190810158tt264
type: Position
type: ExactPosition
position: 264
reference: P10182-1

22572081190810158tt272
type: Position
type: ExactPosition
position: 272
reference: P10182-1

22572081190810158tt276
type: Position
type: ExactPosition
position: 276
reference: P10182-1

22572081190810158tt282
type: Position
type: ExactPosition
position: 282
reference: P10182-1

22572081190810158tt291
type: Position
type: ExactPosition
position: 291
reference: P10182-1

22572081190810158tt293
type: Position
type: ExactPosition
position: 293
reference: P10182-1

22572081190810158tt297
type: Position
type: ExactPosition
position: 297
reference: P10182-1

22572081190810158tt300
type: Position
type: ExactPosition
position: 300
reference: P10182-1

22572081190810158tt310
type: Position
type: ExactPosition
position: 310
reference: P10182-1

22572081190810158tt320
type: Position
type: ExactPosition
position: 320
reference: P10182-1

22572081190810158tt321
type: Position
type: ExactPosition
position: 321
reference: P10182-1

22572081190810158tt323
type: Position
type: ExactPosition
position: 323
reference: P10182-1

22572081190810158tt327
type: Position
type: ExactPosition
position: 327
reference: P10182-1

22572081190810158tt336
type: Position
type: ExactPosition
position: 336
reference: P10182-1

substrate

P10182-1
type: Simple Sequence
modified: 1995-11-01
version: 2
mass: 36200
value: KEVENQQKLANVVILATGGTIAGAGASAANSATYQAAKVGVDKLIAGVPELADLANVRGEQVMQIASESITNDDLLKLGKRVAELADSNDVDGIVITHGTDTLEETAYFLDLTLNTDKPIVVVGSMRPGTAMSADGMLNLYNAVAVASNKDSRGKGVLVTMNDEIQSGRDVSKSINIKTEAFKSAWGPLGMVVEGKSYWFRLPAKRHTVNSEFDIKQISSLPQVDIAYSYGNVTDTAYKALAQNGAKALIHAGTGNGSVSSRLTPALQTLRKTGTQIIRSSHVNQGGFVLRNAEQPDDKNDWVVAHDLNPEKARILVELAMVKTQDSKELQRIFWEY

http://purl.uniprot.org/uniprot/P10182#attribution-609619A7DF3E53CE5E48EDEF8B8F9DD1
manual: true
evidence: ECO_0000250

http://purl.uniprot.org/uniprot/P10182#attribution-DEE029FAFEC36C9A3DB22B3BAA55AD10
manual: true
evidence: ECO_0000255
source: PRU01068

http://purl.uniprot.org/uniprot/P10182#attribution-9F7733B5CDF7129FA0002D840D9B58EF
manual: true
evidence: ECO_0000255
source: PRU10099

http://purl.uniprot.org/uniprot/P10182#attribution-95ED5DAF9A98D5C5CE5584DB38EEB18E
manual: true
evidence: ECO_0000255
source: PRU10100

http://purl.uniprot.org/uniprot/P10182#attribution-E4E7F5AAED3EB276BBF423890489D827
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/10684596

http://purl.uniprot.org/uniprot/P10182#attribution-080ED3982FEC43F7C43A0689717F3D20
manual: true
evidence: ECO_0000305

P80193
type: Protein
reviewed: true
created: 1993-04-01
modified: 2023-09-13
version: 86
mnemonic: BODG_PSESK
citation: http://purl.uniprot.org/citations/8504802
recommended name: SIP65CBB0DC505D6F22
alternative name: SIPB838CAC2D7DD63E1
alternative name: SIP232E50EC1518A8CA
enzyme: http://purl.uniprot.org/enzyme/1.14.11.1
organism: http://purl.uniprot.org/taxonomy/29440
annotation: SIPC05048A9ED1D16E4
annotation: SIP7F97848D00AD0F15
annotation: SIPE2493CA6E408B349
annotation: SIPFC995A8DA840EDAF
annotation: SIP8372E74C0666A623
annotation: SIPE1D16FD6C2D6E574
annotation: SIPF542588C00EF86D0
annotation: SIPEF00E12F2F52AD89
annotation: PRO_0000207089
annotation: SIP0922A8F93DA4F423
annotation: SIP96E55547C6B71ADF
annotation: SIP3AF6721E4200CC14
annotation: SIP6D9AAC385E93E829
annotation: SIP8CBED19C41AAAD56
annotation: SIP86165586DBBAB96B
annotation: SIPE0FF3748D000E8D5
annotation: SIP8845258F8C5396FD
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/124
classified with: http://purl.uniprot.org/keywords/963
classified with: http://purl.uniprot.org/keywords/223
classified with: http://purl.uniprot.org/keywords/903
classified with: http://purl.uniprot.org/keywords/408
classified with: http://purl.uniprot.org/keywords/479
classified with: http://purl.uniprot.org/keywords/862
classified with: GO_0005737
classified with: GO_0008336
classified with: GO_0005506
classified with: GO_0045329
sequence: P80193-1

P80193-citation-8504802
type: Citation Statement
scope: PROTEIN SEQUENCE

http://purl.uniprot.org/brenda/1.14.11.1
type: Resource
database: BRENDA
comment: 5085

cd00250
type: Resource
database: CDD
comment: CAS_like
signature sequence match: CDD cd00250 match 1

http://purl.uniprot.org/gene3d/3.60.130.10
type: Resource
database: Gene3D
comment: Clavaminate synthase-like
signature sequence match: Gene3D 3.60.130.10 match 1

IPR012775
type: Resource
database: InterPro
comment: GBBH-like

IPR010376
type: Resource
database: InterPro
comment: GBBH-like_N

IPR038492
type: Resource
database: InterPro
comment: GBBH-like_N_sf

IPR042098
type: Resource
database: InterPro
comment: TauD-like_sf

IPR003819
type: Resource
database: InterPro
comment: TauD/TfdA-like

TIGR02409
type: Resource
database: NCBIfam
comment: carnitine_bodg
signature sequence match: NCBIfam TIGR02409 match 1

PTHR10696
type: Resource
database: PANTHER
comment: GAMMA-BUTYROBETAINE HYDROXYLASE-RELATED
signature sequence match: PANTHER PTHR10696 match 1

PTHR10696:SF51
type: Resource
database: PANTHER
comment: TRIMETHYLLYSINE DIOXYGENASE, MITOCHONDRIAL
signature sequence match: PANTHER PTHR10696:SF51 match 1

PF06155
type: Resource
database: Pfam
comment: GBBH-like_N
signature sequence match: Pfam PF06155 match 1

PF02668
type: Resource
database: Pfam
comment: TauD
signature sequence match: Pfam PF02668 match 1

SSF51197
type: Resource
database: SUPFAM
comment: Clavaminate synthase-like
signature sequence match: SUPFAM SSF51197 match 1

SIP65CBB0DC505D6F22
type: Structured Name
full name: Gamma-butyrobetaine dioxygenase
ec name: 1.14.11.1

SIPB838CAC2D7DD63E1
type: Structured Name
full name: Gamma-butyrobetaine hydroxylase
short name: Gamma-BBH

SIP232E50EC1518A8CA
type: Structured Name
full name: Gamma-butyrobetaine,2-oxoglutarate dioxygenase

SIPC05048A9ED1D16E4
type: Function Annotation
comment: Catalyzes the formation of L-carnitine from gamma-butyrobetaine.

SIPE2493CA6E408B349
type: Cofactor Annotation
comment: Binds 1 Fe(2+) ion per subunit.
cofactor: CHEBI_29033

SIPE1D16FD6C2D6E574
type: Subunit Annotation
comment: Homodimer.

SIPEF00E12F2F52AD89
type: Similarity Annotation
comment: Belongs to the gamma-BBH/TMLD family.

PRO_0000207089
type: Chain Annotation
comment: Gamma-butyrobetaine dioxygenase
mass: 43322
range: 22579777772270382tt1tt383

22579777772270382tt1
type: Position
type: ExactPosition
position: 1
reference: P80193-1

22579777772270382tt383
type: Position
type: ExactPosition
position: 383
reference: P80193-1

22579777772270382tt46
type: Position
type: ExactPosition
position: 46
reference: P80193-1

22579777772270382tt48
type: Position
type: ExactPosition
position: 48
reference: P80193-1

22579777772270382tt51
type: Position
type: ExactPosition
position: 51
reference: P80193-1

22579777772270382tt91
type: Position
type: ExactPosition
position: 91
reference: P80193-1

22579777772270382tt209
type: Position
type: ExactPosition
position: 209
reference: P80193-1

22579777772270382tt211
type: Position
type: ExactPosition
position: 211
reference: P80193-1

22579777772270382tt350
type: Position
type: ExactPosition
position: 350
reference: P80193-1

SIP8845258F8C5396FD
type: Natural Variant Annotation
comment: In 50% of the chains.
range: 22579777772270382tt1tt1

SIP39D6F52CB3C50ABA
subclass of: CHEBI_24875
comment: catalytic

P80193-1
type: Simple Sequence
modified: 1993-04-01
version: 1
mass: 43322
value: NAIADYRTFPLISPLASAASFASGVSVTWADGRVSPFHNLWLRDNCPCGDCVYEVTREQVFLVADVPEDIQVQAVTIGDDGRLVVQWDDGHASAYHPGWLRAHAYDAQSLAEREAARPHKHRWMQGLSLPVYDHGAVMQDDDTLLEWLLAVRDVGLTQLHGVPTEPGALIPLAKRISFIRESNFGVLFDVRSKADADSNAYTAFNLPLHTDLPTRELQPGLQFLHCLVNDATGGNSTFVDGFAIAEALRIEAPAAYRLLCETPVEFRNKDRHSDYRCTAPVIALDSSGEVREIRLANFLRAPFQMDAQRMPDYYLAYRRFIQMTREPRFCFTRRLEAGQLWCFDNRRVLHARDAFDPASGDRHFQGCYVDRDELLSRILVLQR

http://purl.uniprot.org/uniprot/P80193#attribution-609619A7DF3E53CE5E48EDEF8B8F9DD1
manual: true
evidence: ECO_0000250

http://purl.uniprot.org/uniprot/P80193#attribution-080ED3982FEC43F7C43A0689717F3D20
manual: true
evidence: ECO_0000305

Q52438
type: Protein
reviewed: true
created: 1998-07-15
modified: 2023-05-03
version: 90
mnemonic: BPHA1_PSES1
old mnemonic: BPA1_PSES1
citation: http://purl.uniprot.org/citations/8048958
recommended name: SIPA2AEEEBA7DB312AD
alternative name: SIP06137D0A1F2452C9
enzyme: http://purl.uniprot.org/enzyme/1.14.12.18
organism: http://purl.uniprot.org/taxonomy/307
annotation: SIP01D03B8AA712D150
annotation: SIPF2D101D838EC6979
annotation: SIPE759C12E3B16DA7B
annotation: SIP0A0CF39F0ADC5130
annotation: SIPE2F6CE910E998203
annotation: SIPDCDFEE77EA44CC72
annotation: PRO_0000085049
annotation: SIP4C2D4E8BCB3C0DCD
annotation: SIPE3E4022FF86D9789
annotation: SIP7C14B195638DDE23
annotation: SIP6519F34676AA2DD3
annotation: SIP4A57C31E2A622D51
annotation: SIPA1403E4761424108
annotation: SIP867CB5A57F56DEF9
existence: Inferred from Homology Existence
classified with: http://purl.uniprot.org/keywords/1
classified with: http://purl.uniprot.org/keywords/58
classified with: http://purl.uniprot.org/keywords/223
classified with: http://purl.uniprot.org/keywords/520
classified with: GO_0051537
classified with: GO_0018687
classified with: GO_0005506
classified with: GO_0019439
sequence: Q52438-1

Q52438-citation-8048958
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

http://purl.uniprot.org/brenda/1.14.12.18
type: Resource
database: BRENDA
comment: 5085

cd08881
type: Resource
database: CDD
comment: RHO_alpha_C_NDO-like
signature sequence match: CDD cd08881 match 1

cd03472
type: Resource
database: CDD
comment: Rieske_RO_Alpha_BPDO_like
signature sequence match: CDD cd03472 match 1

http://purl.uniprot.org/gene3d/2.102.10.10
type: Resource
database: Gene3D
comment: Rieske [2Fe-2S] iron-sulphur domain
signature sequence match: Gene3D 2.102.10.10 match 1

IPR043266
type: Resource
database: InterPro
comment: RHO_NdoB-like_C

IPR017941
type: Resource
database: InterPro
comment: Rieske_2Fe-2S

IPR036922
type: Resource
database: InterPro
comment: Rieske_2Fe-2S_sf

IPR015881
type: Resource
database: InterPro
comment: Ring-hydroxy_dOase_2Fe2S_BS

IPR015879
type: Resource
database: InterPro
comment: Ring_hydroxy_dOase_asu_C_dom

IPR001663
type: Resource
database: InterPro
comment: Rng_hydr_dOase-A

PTHR43756:SF1
type: Resource
database: PANTHER
comment: 3-PHENYLPROPIONATE/CINNAMIC ACID DIOXYGENASE SUBUNIT ALPHA
signature sequence match: PANTHER PTHR43756:SF1 match 1

PTHR43756
type: Resource
database: PANTHER
comment: CHOLINE MONOOXYGENASE, CHLOROPLASTIC
signature sequence match: PANTHER PTHR43756 match 1

PF00355
type: Resource
database: Pfam
comment: Rieske
signature sequence match: Pfam PF00355 match 1

PF00848
type: Resource
database: Pfam
comment: Ring_hydroxyl_A
signature sequence match: Pfam PF00848 match 1

PR00090
type: Resource
database: PRINTS
comment: RNGDIOXGNASE

SSF55961
type: Resource
database: SUPFAM
comment: Bet v1-like
signature sequence match: SUPFAM SSF55961 match 1

SSF50022
type: Resource
database: SUPFAM
comment: ISP domain
signature sequence match: SUPFAM SSF50022 match 1

PS51296
type: Resource
database: PROSITE
comment: RIESKE
signature sequence match: PROSITE PS51296 match 1

PS00570
type: Resource
database: PROSITE
comment: RING_HYDROXYL_ALPHA
signature sequence match: PROSITE PS00570 match 1

SIPA2AEEEBA7DB312AD
type: Structured Name
full name: Biphenyl dioxygenase subunit alpha
ec name: 1.14.12.18

SIP06137D0A1F2452C9
type: Structured Name
full name: Biphenyl 2,3-dioxygenase

bphA1
type: Gene

SIPF2D101D838EC6979
type: Cofactor Annotation
comment: Binds 1 [2Fe-2S] cluster per subunit.
cofactor: CHEBI_190135

SIPE759C12E3B16DA7B
type: Cofactor Annotation
comment: Binds 1 Fe cation per subunit.
cofactor: CHEBI_24875

SIPE2F6CE910E998203
type: Subunit Annotation
comment: Heterohexamer consisting of 3 BphA1 subunits and 3 BphA2 subunits. A ferredoxin (BphA3) and a ferredoxin reductase (BphA4) must be present to obtain activity (By similarity).

SIPDCDFEE77EA44CC72
type: Similarity Annotation
comment: Belongs to the bacterial ring-hydroxylating dioxygenase alpha subunit family.

PRO_0000085049
type: Chain Annotation
comment: Biphenyl dioxygenase subunit alpha
mass: 51876
range: 22857962853972014tt1tt458

22857962853972014tt1
type: Position
type: ExactPosition
position: 1
reference: Q52438-1

22857962853972014tt458
type: Position
type: ExactPosition
position: 458
reference: Q52438-1

SIP4C2D4E8BCB3C0DCD
type: Domain Extent Annotation
comment: Rieske
range: 22857962853972014tt59tt157

22857962853972014tt59
type: Position
type: ExactPosition
position: 59
reference: Q52438-1

22857962853972014tt157
type: Position
type: ExactPosition
position: 157
reference: Q52438-1

22857962853972014tt101
type: Position
type: ExactPosition
position: 101
reference: Q52438-1

22857962853972014tt103
type: Position
type: ExactPosition
position: 103
reference: Q52438-1

22857962853972014tt121
type: Position
type: ExactPosition
position: 121
reference: Q52438-1

22857962853972014tt124
type: Position
type: ExactPosition
position: 124
reference: Q52438-1

22857962853972014tt234
type: Position
type: ExactPosition
position: 234
reference: Q52438-1

22857962853972014tt240
type: Position
type: ExactPosition
position: 240
reference: Q52438-1

Q52438-1
type: Simple Sequence
modified: 1996-11-01
version: 1
mass: 51876
value: MGIYSEREVQAVPMTFKRRWTDEAIRALVDQDKGLIDPRIYADQDLYEIELERIFARSWLLLGHEAHIPKTGDYLTTYMGEDPVIMVRQKDGSIKVFLNQCRHRGMRICRSDAGNAKAFTCTYHGWAYDIAGNLVNVPYEKEAFCDKKEGDCGFDKADWGPLQARVETYKGLIFANWDAEAPDLKTYLSDAMPYMDVMLDRTEAGTTVVGGMQKWVIPCNWKFAAEQFCSDMYHAGTMAHLSGVLSSLPPEMDLTQVQMSKNGSQFRAAWGGHGSGWFINDAAILMAVMGPKITQYWTQGPAAEKAAKRLNQMPTQTMFGQHMTVFPTCSFLPGINTIRSWHPRGPNEVECGPSWSSMPMRPEDIKEEFRRQNIRTFNAGGTFEQDDGENWVEIQRGLRGHKAKSAPLCAQMGLNVPNKSNPDFPGKTAYVYAEEAARGMYHHWARMMSEPNWETLKP

http://purl.uniprot.org/uniprot/Q52438#attribution-609619A7DF3E53CE5E48EDEF8B8F9DD1
manual: true
evidence: ECO_0000250

http://purl.uniprot.org/uniprot/Q52438#attribution-FDD5DF45D0B154FC8B4221196591E241
manual: true
evidence: ECO_0000255
source: PRU00628

http://purl.uniprot.org/uniprot/Q52438#attribution-080ED3982FEC43F7C43A0689717F3D20
manual: true
evidence: ECO_0000305

Q52439
type: Protein
reviewed: true
created: 1998-07-15
modified: 2023-05-03
version: 72
mnemonic: BPHA2_PSES1
old mnemonic: BPA2_PSES1
citation: http://purl.uniprot.org/citations/8048958
recommended name: SIP4AF980A9BED57661
alternative name: SIP06137D0A1F2452C9
enzyme: http://purl.uniprot.org/enzyme/1.14.12.18
organism: http://purl.uniprot.org/taxonomy/307
annotation: SIPE3928CAC77EF96C8
annotation: SIP0A3519AD0B7C950C
annotation: SIP21E62A48F23C5235
annotation: SIP6E724EBA10764501
annotation: SIPB098E2E3119F06A8
annotation: PRO_0000085070
existence: Inferred from Homology Existence
classified with: http://purl.uniprot.org/keywords/58
classified with: http://purl.uniprot.org/keywords/223
classified with: http://purl.uniprot.org/keywords/520
classified with: GO_0018687
classified with: GO_0019439
classified with: GO_0071704
sequence: MTRVAMAQKTAELIQEFAWPAQPVSPQLQHEVEQFYYREAQLLDHHNYNAWFDLLEQDIHYWMPIRTTRIKRENDKEYIPAGANAHFDENHETMRGRIRQRTSELNWAEDPLSRTRHIVSNVIVRETDTPATLEVASAFLVYRNRLERQVDIFAGERRDVLRRANNGLGFKIARRTILIDQSTILANNLSVFF

Q52439-citation-8048958
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

cd00667
type: Resource
database: CDD
comment: ring_hydroxylating_dioxygenases_beta
signature sequence match: CDD cd00667 match 1

IPR032710
type: Resource
database: InterPro
comment: NTF2-like_dom_sf

IPR000391
type: Resource
database: InterPro
comment: Rng_hydr_dOase-bsu

PTHR41534:SF2
type: Resource
database: PANTHER
comment: 3-PHENYLPROPIONATE/CINNAMIC ACID DIOXYGENASE SUBUNIT BETA
signature sequence match: PANTHER PTHR41534:SF2 match 1

PTHR41534
type: Resource
database: PANTHER
comment: BLR3401 PROTEIN
signature sequence match: PANTHER PTHR41534 match 1

PF00866
type: Resource
database: Pfam
comment: Ring_hydroxyl_B
signature sequence match: Pfam PF00866 match 1

SSF54427
type: Resource
database: SUPFAM
comment: NTF2-like
signature sequence match: SUPFAM SSF54427 match 1

SIP4AF980A9BED57661
type: Structured Name
full name: Biphenyl dioxygenase subunit beta
ec name: 1.14.12.18

SIP06137D0A1F2452C9
type: Structured Name
full name: Biphenyl 2,3-dioxygenase

bphA2
type: Gene

SIPE3928CAC77EF96C8
type: Function Annotation
comment: The beta subunit may be responsible for the substrate specificity of the enzyme.

SIP6E724EBA10764501
type: Subunit Annotation
comment: Heterohexamer consisting of 3 BphA1 subunits and 3 BphA2 subunits. A ferredoxin (BphA3) and a ferredoxin reductase (BphA4) must be present to obtain activity.

SIPB098E2E3119F06A8
type: Similarity Annotation
comment: Belongs to the bacterial ring-hydroxylating dioxygenase beta subunit family.

PRO_0000085070
type: Chain Annotation
comment: Biphenyl dioxygenase subunit beta
mass: 22779
range: 22857962853972270tt1tt193

22857962853972270tt1
type: Position
type: ExactPosition
position: 1
reference: MTRVAMAQKTAELIQEFAWPAQPVSPQLQHEVEQFYYREAQLLDHHNYNAWFDLLEQDIHYWMPIRTTRIKRENDKEYIPAGANAHFDENHETMRGRIRQRTSELNWAEDPLSRTRHIVSNVIVRETDTPATLEVASAFLVYRNRLERQVDIFAGERRDVLRRANNGLGFKIARRTILIDQSTILANNLSVFF

22857962853972270tt193
type: Position
type: ExactPosition
position: 193
reference: MTRVAMAQKTAELIQEFAWPAQPVSPQLQHEVEQFYYREAQLLDHHNYNAWFDLLEQDIHYWMPIRTTRIKRENDKEYIPAGANAHFDENHETMRGRIRQRTSELNWAEDPLSRTRHIVSNVIVRETDTPATLEVASAFLVYRNRLERQVDIFAGERRDVLRRANNGLGFKIARRTILIDQSTILANNLSVFF

MTRVAMAQKTAELIQEFAWPAQPVSPQLQHEVEQFYYREAQLLDHHNYNAWFDLLEQDIHYWMPIRTTRIKRENDKEYIPAGANAHFDENHETMRGRIRQRTSELNWAEDPLSRTRHIVSNVIVRETDTPATLEVASAFLVYRNRLERQVDIFAGERRDVLRRANNGLGFKIARRTILIDQSTILANNLSVFF
type: Simple Sequence
modified: 1996-11-01
version: 1
mass: 22779

http://purl.uniprot.org/uniprot/Q52439#attribution-080ED3982FEC43F7C43A0689717F3D20
manual: true
evidence: ECO_0000305

Q52440
type: Protein
reviewed: true
created: 1998-07-15
modified: 2023-05-03
version: 72
mnemonic: BPHA3_PSES1
old mnemonic: BPA3_PSES1
citation: http://purl.uniprot.org/citations/8048958
recommended name: SIPA00E1541C59154DD
organism: http://purl.uniprot.org/taxonomy/307
annotation: SIP6C389D1C94C7E21E
annotation: SIPCA96F91F52CDB60B
annotation: SIP0E0BF7CF85DB62EC
annotation: PRO_0000201687
annotation: SIPE89C69134FED7653
annotation: SIPFFF74F3B327DFA80
annotation: SIPCF09F016D26AF145
annotation: SIP8787E7D4FFDAE783
annotation: SIP64B92165CF716D8A
annotation: SIP678C4DEA3AC31CAE
annotation: SIP554BC89334C3D751
annotation: SIP9D5FC3E7FB892863
annotation: SIP123EA2C8C706A571
annotation: SIPB2F4587BCAA9C4EC
annotation: SIP67F7696A73D67636
annotation: SIP397092E8DD897F38
annotation: SIPCA8F5405EC143DF8
annotation: SIP4C511418913FDCA8
annotation: SIP8E8F28FC9E2CDA45
annotation: SIP533AB6680D155864
annotation: SIP46070EF209A0256B
annotation: SIP1894208C607B51B9
annotation: SIPBC0391A287C36439
annotation: SIP7FB72DB91C81916B
annotation: SIP11F8BF43E4907102
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/1
classified with: http://purl.uniprot.org/keywords/2
classified with: http://purl.uniprot.org/keywords/58
classified with: http://purl.uniprot.org/keywords/249
classified with: GO_0051537
classified with: GO_0046872
classified with: GO_0019439
sequence: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

Q52440-citation-8048958
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

2E4P
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.00
chain sequence mapping: PDB 2E4P tt1tt109

PDB 2E4P tt1tt109
chain: A/B=1-109

2E4Q
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 1.80
chain sequence mapping: PDB 2E4Q tt1tt109

PDB 2E4Q tt1tt109
chain: A/C=1-109

2YVJ
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 1.90
chain sequence mapping: PDB 2YVJ tt1tt109

PDB 2YVJ tt1tt109
chain: B=1-109

cd03528
type: Resource
database: CDD
comment: Rieske_RO_ferredoxin
signature sequence match: CDD cd03528 match 1

PTHR21496:SF23
type: Resource
database: PANTHER
comment: 3-PHENYLPROPIONATE/CINNAMIC ACID DIOXYGENASE FERREDOXIN SUBUNIT
signature sequence match: PANTHER PTHR21496:SF23 match 1

PTHR21496
type: Resource
database: PANTHER
comment: FERREDOXIN-RELATED
signature sequence match: PANTHER PTHR21496 match 1

SIPA00E1541C59154DD
type: Structured Name
full name: Biphenyl dioxygenase ferredoxin subunit

bphA3
type: Gene

SIP6C389D1C94C7E21E
type: Function Annotation
comment: This protein seems to be a 2Fe-2S ferredoxin.

SIPCA96F91F52CDB60B
type: Subunit Annotation
comment: This dioxygenase system consists of four proteins: the two subunits of the hydroxylase component (BphA1 and BphA2), a ferredoxin (BphA3) and a ferredoxin reductase (BphA4).

SIP0E0BF7CF85DB62EC
type: Similarity Annotation
comment: Belongs to the bacterial ring-hydroxylating dioxygenase ferredoxin component family.

PRO_0000201687
type: Chain Annotation
comment: Biphenyl dioxygenase ferredoxin subunit
mass: 11913
range: 22857962854035502tt1tt109

22857962854035502tt1
type: Position
type: ExactPosition
position: 1
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt109
type: Position
type: ExactPosition
position: 109
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

SIPE89C69134FED7653
type: Domain Extent Annotation
comment: Rieske
range: 22857962854035502tt4tt100

22857962854035502tt4
type: Position
type: ExactPosition
position: 4
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt100
type: Position
type: ExactPosition
position: 100
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt43
type: Position
type: ExactPosition
position: 43
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt45
type: Position
type: ExactPosition
position: 45
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt63
type: Position
type: ExactPosition
position: 63
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt66
type: Position
type: ExactPosition
position: 66
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt8
type: Position
type: ExactPosition
position: 8
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt9
type: Position
type: ExactPosition
position: 9
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt11
type: Position
type: ExactPosition
position: 11
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt17
type: Position
type: ExactPosition
position: 17
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt22
type: Position
type: ExactPosition
position: 22
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt25
type: Position
type: ExactPosition
position: 25
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt32
type: Position
type: ExactPosition
position: 32
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt35
type: Position
type: ExactPosition
position: 35
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt42
type: Position
type: ExactPosition
position: 42
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt44
type: Position
type: ExactPosition
position: 44
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt46
type: Position
type: ExactPosition
position: 46
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt51
type: Position
type: ExactPosition
position: 51
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt53
type: Position
type: ExactPosition
position: 53
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt55
type: Position
type: ExactPosition
position: 55
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt57
type: Position
type: ExactPosition
position: 57
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt60
type: Position
type: ExactPosition
position: 60
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt62
type: Position
type: ExactPosition
position: 62
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt64
type: Position
type: ExactPosition
position: 64
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt69
type: Position
type: ExactPosition
position: 69
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt71
type: Position
type: ExactPosition
position: 71
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt72
type: Position
type: ExactPosition
position: 72
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt74
type: Position
type: ExactPosition
position: 74
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt77
type: Position
type: ExactPosition
position: 77
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt81
type: Position
type: ExactPosition
position: 81
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt91
type: Position
type: ExactPosition
position: 91
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt94
type: Position
type: ExactPosition
position: 94
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt97
type: Position
type: ExactPosition
position: 97
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt102
type: Position
type: ExactPosition
position: 102
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

22857962854035502tt104
type: Position
type: ExactPosition
position: 104
reference: MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA

MTFTKACSVDEVPPGEALQVSHDAQKVAIFNVDGEFFATQDQCTHGEWSLSEGGYLDGDVVECSLHMGKFCVRTGKVKSPPPCEPLKVYPIRIEGRDVLVDFSRAALHA
type: Simple Sequence
modified: 1996-11-01
version: 1
mass: 11913

http://purl.uniprot.org/uniprot/Q52440#attribution-FDD5DF45D0B154FC8B4221196591E241
manual: true
evidence: ECO_0000255
source: PRU00628

http://purl.uniprot.org/uniprot/Q52440#attribution-080ED3982FEC43F7C43A0689717F3D20
manual: true
evidence: ECO_0000305

P50206
type: Protein
reviewed: true
created: 1996-10-01
modified: 2023-09-13
version: 87
mnemonic: BPHB_PSES1
citation: http://purl.uniprot.org/citations/8048958
recommended name: SIP4758C8A635B7CAA5
alternative name: SIP6524A4412653F9C4
alternative name: SIP129ACE5A97F83313
alternative name: SIPE426E9E77F06E505
alternative name: SIPCE90861E864593F0
enzyme: http://purl.uniprot.org/enzyme/1.3.1.56
organism: http://purl.uniprot.org/taxonomy/307
annotation: SIP1BC43856A96AA0F5
annotation: SIPE1E78113522DDC9B
annotation: SIP7559DB2C2ACF8539
annotation: PRO_0000054534
annotation: SIP8FE670C5D012F316
annotation: SIP6B057CC6B9CB1972
annotation: SIPE48136005C37490C
existence: Inferred from Homology Existence
classified with: http://purl.uniprot.org/keywords/58
classified with: http://purl.uniprot.org/keywords/520
classified with: http://purl.uniprot.org/keywords/560
classified with: GO_0018509
classified with: GO_0019439
sequence: P50206-1

P50206-citation-8048958
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

cd05348
type: Resource
database: CDD
comment: BphB-like_SDR_c
signature sequence match: CDD cd05348 match 1

IPR017711
type: Resource
database: InterPro
comment: BphB_TodD

IPR020904
type: Resource
database: InterPro
comment: Sc_DH/Rdtase_CS

IPR002347
type: Resource
database: InterPro
comment: SDR_fam

TIGR03325
type: Resource
database: NCBIfam
comment: BphB_TodD
signature sequence match: NCBIfam TIGR03325 match 1

PTHR43008
type: Resource
database: PANTHER
comment: BENZIL REDUCTASE
signature sequence match: PANTHER PTHR43008 match 1

PTHR43008:SF4
type: Resource
database: PANTHER
comment: CHAIN DEHYDROGENASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G08710)-RELATED
signature sequence match: PANTHER PTHR43008:SF4 match 1

PF00106
type: Resource
database: Pfam
comment: adh_short
signature sequence match: Pfam PF00106 match 1

PR00081
type: Resource
database: PRINTS
comment: GDHRDH

PR00080
type: Resource
database: PRINTS
comment: SDRFAMILY

PS00061
type: Resource
database: PROSITE
comment: ADH_SHORT
signature sequence match: PROSITE PS00061 match 1

SIP4758C8A635B7CAA5
type: Structured Name
full name: Cis-2,3-dihydrobiphenyl-2,3-diol dehydrogenase
ec name: 1.3.1.56

SIP6524A4412653F9C4
type: Structured Name
full name: 2,3-dihydro-2,3-dihydroxybiphenyl dehydrogenase

SIP129ACE5A97F83313
type: Structured Name
full name: 2,3-dihydroxy-4-phenylhexa-4,6-diene dehydrogenase

SIPE426E9E77F06E505
type: Structured Name
full name: Biphenyl-2,3-dihydro-2,3-diol dehydrogenase

SIPCE90861E864593F0
type: Structured Name
full name: Biphenyl-cis-diol dehydrogenase

bphB
type: Gene

SIP7559DB2C2ACF8539
type: Similarity Annotation
comment: Belongs to the short-chain dehydrogenases/reductases (SDR) family.

PRO_0000054534
type: Chain Annotation
comment: Cis-2,3-dihydrobiphenyl-2,3-diol dehydrogenase
mass: 28933
range: 22576479253575214tt1tt276

22576479253575214tt1
type: Position
type: ExactPosition
position: 1
reference: P50206-1

22576479253575214tt276
type: Position
type: ExactPosition
position: 276
reference: P50206-1

SIP8FE670C5D012F316
type: Active Site Annotation
comment: Proton acceptor
range: 22576479253575214tt155tt155

22576479253575214tt155
type: Position
type: ExactPosition
position: 155
reference: P50206-1

22576479253575214tt9
type: Position
type: ExactPosition
position: 9
reference: P50206-1

22576479253575214tt33
type: Position
type: ExactPosition
position: 33
reference: P50206-1

22576479253575214tt142
type: Position
type: ExactPosition
position: 142
reference: P50206-1

substrate

P50206-1
type: Simple Sequence
modified: 1996-10-01
version: 1
mass: 28933
value: MQLNNEVALVTGGGSGLGRAIVDRFVAEGARVAVLDKSAARLQELQAAHGAKVLGIEGDVRVLADHQKAARECVAAFGKIDCLIPNAGIWDYSMPLVDIPDDRIDAAFDEVFHINVKGYLLAVKACLPALVQSRGSVVFTISNAGFYPNGGGPLYTGAKHAVVGMVRELAYELAPHVRVNGVAPGGMSTDLRGPASLGMANQAISSVPLGEMLTSVLPVGRMPVRAEYTGAYVFFATRGDTFPTTGALLNHDGGMGVRGFFEATGGKDLPQKLRLS

http://purl.uniprot.org/uniprot/P50206#attribution-609619A7DF3E53CE5E48EDEF8B8F9DD1
manual: true
evidence: ECO_0000250

http://purl.uniprot.org/uniprot/P50206#attribution-71E089CAE4A445B16847142C8E7D2A2D
manual: true
evidence: ECO_0000255
source: PRU10001

http://purl.uniprot.org/uniprot/P50206#attribution-080ED3982FEC43F7C43A0689717F3D20
manual: true
evidence: ECO_0000305

P17297
type: Protein
reviewed: true
created: 1990-08-01
modified: 2023-09-13
version: 118
mnemonic: BPHC_PSES1
old mnemonic: BPHC_PSESP
replaces: Q52441
citation: http://purl.uniprot.org/citations/2540155
citation: http://purl.uniprot.org/citations/8048958
citation: http://purl.uniprot.org/citations/8636975
citation: http://purl.uniprot.org/citations/11293547
citation: http://purl.uniprot.org/citations/12206778
recommended name: SIP71D827B41363451F
alternative name: SIP82DDA4E41BF35265
alternative name: SIP42943C32D384CDA0
enzyme: http://purl.uniprot.org/enzyme/1.13.11.39
organism: http://purl.uniprot.org/taxonomy/307
annotation: SIP4C01CD3067F28F43
annotation: SIP44DE6E27C604F3C0
annotation: SIPA2A011D1315505F2
annotation: SIP37888890934B62BA
annotation: SIPEACD4BF77725A69C
annotation: SIPD982965DDE65EF9B
annotation: PRO_0000085035
annotation: SIPB8B251CFF7781ADD
annotation: SIPCEEAEFF4C862277B
annotation: SIP62E56634E65711A3
annotation: SIP23F664AD50DB553F
annotation: SIPA41099D39791EBFC
annotation: SIPCAF30E78E9294106
annotation: SIPC0E28F2EFEEE8AB6
annotation: SIP9037F526D86BA860
annotation: SIPB84CCC9583E3DE37
annotation: SIP3A80E32663117B63
annotation: SIP8A9D8E212ABB06E8
annotation: SIPDE70A6757ED17E41
annotation: SIP4D3B13AF750B0978
annotation: SIP025A1DF7F71400C5
annotation: SIP738FDFA608D24B2B
annotation: SIP6C701C3C191877EB
annotation: SIPA881074BF2A2A9D6
annotation: SIP9D79EF8629ED5343
annotation: SIPD60420294A121A55
annotation: SIP8DB59AFF4BEBBDA7
annotation: SIP10992BAB4E0DDF87
annotation: SIPAC63ACD1BBE786F3
annotation: SIP9F4B85A36FEBC8F9
annotation: SIPBD54CE11515BDDB9
annotation: SIP55B1CE0DDCD3E15B
annotation: SIPD73053F79A56CCF9
annotation: SIPC2173FE5C21CBE3D
annotation: SIP42595BA7DC04C308
annotation: SIPC15F53AA8E91CACF
annotation: SIP5041678A22D501C6
annotation: SIPE5AA963D4D6CEAD4
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/2
classified with: http://purl.uniprot.org/keywords/58
classified with: http://purl.uniprot.org/keywords/223
classified with: http://purl.uniprot.org/keywords/408
classified with: http://purl.uniprot.org/keywords/479
classified with: http://purl.uniprot.org/keywords/677
classified with: GO_0018583
classified with: GO_0008198
classified with: GO_0019439
classified with: GO_0042178
sequence: P17297-1

P17297-citation-2540155
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

P17297-citation-8048958
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-16

P17297-citation-8636975
type: Citation Statement
scope: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS)

P17297-citation-11293547
type: Citation Statement
scope: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS)

P17297-citation-12206778
type: Citation Statement
scope: X-RAY CRYSTALLOGRAPHY (1.45 ANGSTROMS) OF NATIVE ENZYME; SUBSTRATE COMPLEXES AND H145A MUTANT

A32312
type: Resource
database: PIR
comment: DAPSPC

1DHY
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.30
chain sequence mapping: PDB 1DHY tt2tt293

PDB 1DHY tt2tt293
chain: A=2-293

1EIL
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.00
chain sequence mapping: PDB 1EIL tt2tt293

PDB 1EIL tt2tt293
chain: A=2-293

1EIQ
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.00
chain sequence mapping: PDB 1EIQ tt2tt293

PDB 1EIQ tt2tt293
chain: A=2-293

1EIR
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.00
chain sequence mapping: PDB 1EIR tt2tt293

PDB 1EIR tt2tt293
chain: A=2-293

1KW3
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 1.45
chain sequence mapping: PDB 1KW3 tt2tt293

PDB 1KW3 tt2tt293
chain: B=2-293

1KW6
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 1.45
chain sequence mapping: PDB 1KW6 tt2tt293

PDB 1KW6 tt2tt293
chain: B=2-293

1KW8
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.00
chain sequence mapping: PDB 1KW8 tt2tt293

PDB 1KW8 tt2tt293
chain: B=2-293

1KW9
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 1.95
chain sequence mapping: PDB 1KW9 tt2tt293

PDB 1KW9 tt2tt293
chain: B=2-293

1KWB
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.00
chain sequence mapping: PDB 1KWB tt2tt293

PDB 1KWB tt2tt293
chain: B=2-293

1KWC
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.10
chain sequence mapping: PDB 1KWC tt2tt293

PDB 1KWC tt2tt293
chain: B=2-293

DB02923
type: Resource
database: DrugBank
comment: Biphenyl-2,3-Diol

cd07237
type: Resource
database: CDD
comment: BphC1-RGP6_C_like
signature sequence match: CDD cd07237 match 1

cd07252
type: Resource
database: CDD
comment: BphC1-RGP6_N_like
signature sequence match: CDD cd07252 match 1

http://purl.uniprot.org/gene3d/3.10.180.10
type: Resource
database: Gene3D
comment: 2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1
signature sequence match: Gene3D 3.10.180.10 match 1
signature sequence match: Gene3D 3.10.180.10 match 2

IPR017626
type: Resource
database: InterPro
comment: DiOHbiphenyl_dOase

IPR029068
type: Resource
database: InterPro
comment: Glyas_Bleomycin-R_OHBP_Dase

IPR004360
type: Resource
database: InterPro
comment: Glyas_Fos-R_dOase_dom

IPR037523
type: Resource
database: InterPro
comment: VOC

IPR000486
type: Resource
database: InterPro
comment: Xdiol_ring_cleave_dOase_1/2

TIGR03213
type: Resource
database: NCBIfam
comment: 23dbph12diox
signature sequence match: NCBIfam TIGR03213 match 1

PF00903
type: Resource
database: Pfam
comment: Glyoxalase
signature sequence match: Pfam PF00903 match 1

SSF54593
type: Resource
database: SUPFAM
comment: Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase
signature sequence match: SUPFAM SSF54593 match 1
signature sequence match: SUPFAM SSF54593 match 2

PS00082
type: Resource
database: PROSITE
comment: EXTRADIOL_DIOXYGENAS
signature sequence match: PROSITE PS00082 match 1

PS51819
type: Resource
database: PROSITE
comment: VOC
signature sequence match: PROSITE PS51819 match 1
signature sequence match: PROSITE PS51819 match 2

SIP71D827B41363451F
type: Structured Name
full name: Biphenyl-2,3-diol 1,2-dioxygenase
ec name: 1.13.11.39

SIP82DDA4E41BF35265
type: Structured Name
full name: 2,3-dihydroxybiphenyl dioxygenase
short name: DHBD

SIP42943C32D384CDA0
type: Structured Name
full name: 23OHBP oxygenase

bphC
type: Gene

SIP37888890934B62BA
type: Subunit Annotation
comment: Homooctamer.

SIPEACD4BF77725A69C
type: Similarity Annotation
comment: Belongs to the extradiol ring-cleavage dioxygenase family.

SIPD982965DDE65EF9B
type: Initiator Methionine Annotation
comment: Removed
range: 22572111272425262tt1tt1

22572111272425262tt1
type: Position
type: ExactPosition
position: 1
reference: P17297-1

PRO_0000085035
type: Chain Annotation
comment: Biphenyl-2,3-diol 1,2-dioxygenase
mass: 32114
range: 22572111272425262tt2tt293

22572111272425262tt2
type: Position
type: ExactPosition
position: 2
reference: P17297-1

22572111272425262tt293
type: Position
type: ExactPosition
position: 293
reference: P17297-1

SIPB8B251CFF7781ADD
type: Domain Extent Annotation
comment: VOC 1
range: 22572111272425262tt5tt119

22572111272425262tt5
type: Position
type: ExactPosition
position: 5
reference: P17297-1

22572111272425262tt119
type: Position
type: ExactPosition
position: 119
reference: P17297-1

SIPCEEAEFF4C862277B
type: Domain Extent Annotation
comment: VOC 2
range: 22572111272425262tt143tt265

22572111272425262tt143
type: Position
type: ExactPosition
position: 143
reference: P17297-1

22572111272425262tt265
type: Position
type: ExactPosition
position: 265
reference: P17297-1

22572111272425262tt146
type: Position
type: ExactPosition
position: 146
reference: P17297-1

22572111272425262tt210
type: Position
type: ExactPosition
position: 210
reference: P17297-1

22572111272425262tt261
type: Position
type: ExactPosition
position: 261
reference: P17297-1

22572111272425262tt14
type: Position
type: ExactPosition
position: 14
reference: P17297-1

22572111272425262tt16
type: Position
type: ExactPosition
position: 16
reference: P17297-1

22572111272425262tt25
type: Position
type: ExactPosition
position: 25
reference: P17297-1

22572111272425262tt30
type: Position
type: ExactPosition
position: 30
reference: P17297-1

22572111272425262tt35
type: Position
type: ExactPosition
position: 35
reference: P17297-1

22572111272425262tt38
type: Position
type: ExactPosition
position: 38
reference: P17297-1

22572111272425262tt47
type: Position
type: ExactPosition
position: 47
reference: P17297-1

22572111272425262tt49
type: Position
type: ExactPosition
position: 49
reference: P17297-1

22572111272425262tt54
type: Position
type: ExactPosition
position: 54
reference: P17297-1

22572111272425262tt59
type: Position
type: ExactPosition
position: 59
reference: P17297-1

22572111272425262tt66
type: Position
type: ExactPosition
position: 66
reference: P17297-1

22572111272425262tt70
type: Position
type: ExactPosition
position: 70
reference: P17297-1

22572111272425262tt83
type: Position
type: ExactPosition
position: 83
reference: P17297-1

22572111272425262tt87
type: Position
type: ExactPosition
position: 87
reference: P17297-1

22572111272425262tt89
type: Position
type: ExactPosition
position: 89
reference: P17297-1

22572111272425262tt92
type: Position
type: ExactPosition
position: 92
reference: P17297-1

22572111272425262tt98
type: Position
type: ExactPosition
position: 98
reference: P17297-1

22572111272425262tt101
type: Position
type: ExactPosition
position: 101
reference: P17297-1

22572111272425262tt107
type: Position
type: ExactPosition
position: 107
reference: P17297-1

22572111272425262tt109
type: Position
type: ExactPosition
position: 109
reference: P17297-1

22572111272425262tt111
type: Position
type: ExactPosition
position: 111
reference: P17297-1

22572111272425262tt113
type: Position
type: ExactPosition
position: 113
reference: P17297-1

22572111272425262tt118
type: Position
type: ExactPosition
position: 118
reference: P17297-1

22572111272425262tt131
type: Position
type: ExactPosition
position: 131
reference: P17297-1

22572111272425262tt133
type: Position
type: ExactPosition
position: 133
reference: P17297-1

22572111272425262tt140
type: Position
type: ExactPosition
position: 140
reference: P17297-1

22572111272425262tt142
type: Position
type: ExactPosition
position: 142
reference: P17297-1

22572111272425262tt150
type: Position
type: ExactPosition
position: 150
reference: P17297-1

22572111272425262tt154
type: Position
type: ExactPosition
position: 154
reference: P17297-1

22572111272425262tt163
type: Position
type: ExactPosition
position: 163
reference: P17297-1

22572111272425262tt168
type: Position
type: ExactPosition
position: 168
reference: P17297-1

22572111272425262tt178
type: Position
type: ExactPosition
position: 178
reference: P17297-1

22572111272425262tt181
type: Position
type: ExactPosition
position: 181
reference: P17297-1

22572111272425262tt194
type: Position
type: ExactPosition
position: 194
reference: P17297-1

22572111272425262tt196
type: Position
type: ExactPosition
position: 196
reference: P17297-1

22572111272425262tt200
type: Position
type: ExactPosition
position: 200
reference: P17297-1

22572111272425262tt205
type: Position
type: ExactPosition
position: 205
reference: P17297-1

22572111272425262tt217
type: Position
type: ExactPosition
position: 217
reference: P17297-1

22572111272425262tt218
type: Position
type: ExactPosition
position: 218
reference: P17297-1

22572111272425262tt230
type: Position
type: ExactPosition
position: 230
reference: P17297-1

22572111272425262tt238
type: Position
type: ExactPosition
position: 238
reference: P17297-1

22572111272425262tt244
type: Position
type: ExactPosition
position: 244
reference: P17297-1

22572111272425262tt247
type: Position
type: ExactPosition
position: 247
reference: P17297-1

22572111272425262tt252
type: Position
type: ExactPosition
position: 252
reference: P17297-1

22572111272425262tt259
type: Position
type: ExactPosition
position: 259
reference: P17297-1

22572111272425262tt264
type: Position
type: ExactPosition
position: 264
reference: P17297-1

22572111272425262tt277
type: Position
type: ExactPosition
position: 277
reference: P17297-1

22572111272425262tt279
type: Position
type: ExactPosition
position: 279
reference: P17297-1

22572111272425262tt281
type: Position
type: ExactPosition
position: 281
reference: P17297-1

22572111272425262tt285
type: Position
type: ExactPosition
position: 285
reference: P17297-1

P17297-1
type: Simple Sequence
modified: 2007-01-23
version: 3
mass: 32245
value: MSIERLGYLGFAVKDVPAWDHFLTKSVGLMAAGSAGDAALYRADQRAWRIAVQPGELDDLAYAGLEVDDAAALERMADKLRQAGVAFTRGDEALMQQRKVMGLLCLQDPFGLPLEIYYGPAEIFHEPFLPSAPVSGFVTGDQGIGHFVRCVPDTAKAMAFYTEVLGFVLSDIIDIQMGPETSVPAHFLHCNGRHHTIALAAFPIPKRIHHFMLQANTIDDVGYAFDRLDAAGRITSLLGRHTNDQTLSFYADTPSPMIEVEFGWGPRTVDSSWTVARHSRTAMWGHKSVRGQR

http://purl.uniprot.org/uniprot/P17297#attribution-05C16B64E576893F0FB5E6F0F0FB0983
manual: true
evidence: ECO_0000255
source: PRU01163

http://purl.uniprot.org/uniprot/P17297#attribution-080ED3982FEC43F7C43A0689717F3D20
manual: true
evidence: ECO_0000305

P17548
type: Protein
reviewed: true
created: 1990-08-01
modified: 2023-09-13
version: 82
mnemonic: BPHD_PSES1
old mnemonic: BPHD_PSESP
citation: http://purl.uniprot.org/citations/2540155
conflicting sequence: SIP6563484F39D4EAD0
recommended name: SIP626AB1CCD6EEEFD1
alternative name: SIPDA13AB3CD1959653
enzyme: http://purl.uniprot.org/enzyme/3.7.1.8
organism: http://purl.uniprot.org/taxonomy/307
annotation: SIP88AF1266A9F24A12
annotation: SIPE3E69450BAF1B28B
annotation: SIP6BE3C4C3F9858781
annotation: SIP59B5AB953DFB5240
annotation: SIP78B8FB0402779CF9
annotation: SIP9F2E2989493AA5CE
annotation: PRO_0000207053
annotation: SIPAAD9654A0CE86AD8
annotation: SIP5156ED7AA8D498FB
annotation: SIP5A0A647EB15D2D4C
annotation: SIP5A484653235C4C82
annotation: SIP4310D1022D34034C
annotation: SIPF6FA32A87B3F359C
annotation: SIPC147F76E28AE2E43
annotation: SIPA5AE0BB1B11B996B
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/58
classified with: http://purl.uniprot.org/keywords/903
classified with: http://purl.uniprot.org/keywords/378
classified with: GO_0018774
classified with: GO_0018771
classified with: GO_0019439
sequence: P17548-1

P17548-citation-2540155
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
scope: PROTEIN SEQUENCE OF 2-33
scope: FUNCTION

EMBL AAA25751.1
comment: Frameshift.

B32312
type: Resource
database: PIR
comment: ESPSSK

psesl-bphD
type: Resource
database: ESTHER
comment: Carbon-carbon_bond_hydrolase

http://purl.uniprot.org/gene3d/3.40.50.1820
type: Resource
database: Gene3D
comment: alpha/beta hydrolase
signature sequence match: Gene3D 3.40.50.1820 match 1

MF_01688
type: Resource
database: HAMAP
comment: Biphenyl_BphD
signature sequence match: HAMAP MF 01688 match 1

IPR029058
type: Resource
database: InterPro
comment: AB_hydrolase

IPR000073
type: Resource
database: InterPro
comment: AB_hydrolase_1

IPR000639
type: Resource
database: InterPro
comment: Epox_hydrolase-like

IPR017727
type: Resource
database: InterPro
comment: HOPD_hydrolase_BphD

TIGR03343
type: Resource
database: NCBIfam
comment: biphenyl_bphD
signature sequence match: NCBIfam TIGR03343 match 1

PTHR43689:SF8
type: Resource
database: PANTHER
comment: ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN
signature sequence match: PANTHER PTHR43689:SF8 match 1

PTHR43689
type: Resource
database: PANTHER
comment: HYDROLASE
signature sequence match: PANTHER PTHR43689 match 1

PF00561
type: Resource
database: Pfam
comment: Abhydrolase_1
signature sequence match: Pfam PF00561 match 1

PR00111
type: Resource
database: PRINTS
comment: ABHYDROLASE

PR00412
type: Resource
database: PRINTS
comment: EPOXHYDRLASE

SSF53474
type: Resource
database: SUPFAM
comment: alpha/beta-Hydrolases
signature sequence match: SUPFAM SSF53474 match 1

SIP626AB1CCD6EEEFD1
type: Structured Name
full name: 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase
short name: HOPDA hydrolase
ec name: 3.7.1.8

SIPDA13AB3CD1959653
type: Structured Name
full name: 2,6-dioxo-6-phenylhexa-3-enoate hydrolase

bphD
type: Gene

SIP88AF1266A9F24A12
type: Function Annotation
comment: Catalyzes an unusual C-C bond hydrolysis of 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid (HOPDA) to produce benzoic acid and 2-hydroxy-2,4-pentadienoic acid (HPD).

SIP59B5AB953DFB5240
type: Subunit Annotation
comment: Homodimer.

SIP78B8FB0402779CF9
type: Similarity Annotation
comment: Belongs to the AB hydrolase superfamily. BphD family.

PRO_0000207053
type: Chain Annotation
comment: 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase
mass: 31589
range: 22572111322429486tt1tt286

22572111322429486tt1
type: Position
type: ExactPosition
position: 1
reference: P17548-1

22572111322429486tt286
type: Position
type: ExactPosition
position: 286
reference: P17548-1

SIPAAD9654A0CE86AD8
type: Active Site Annotation
comment: Proton acceptor
range: 22572111322429486tt265tt265

22572111322429486tt265
type: Position
type: ExactPosition
position: 265
reference: P17548-1

22572111322429486tt42
type: Position
type: ExactPosition
position: 42
reference: P17548-1

22572111322429486tt43
type: Position
type: ExactPosition
position: 43
reference: P17548-1

22572111322429486tt51
type: Position
type: ExactPosition
position: 51
reference: P17548-1

22572111322429486tt111
type: Position
type: ExactPosition
position: 111
reference: P17548-1

22572111322429486tt180
type: Position
type: ExactPosition
position: 180
reference: P17548-1

22572111322429486tt190
type: Position
type: ExactPosition
position: 190
reference: P17548-1

22572111322429486tt266
type: Position
type: ExactPosition
position: 266
reference: P17548-1

SIPA5AE0BB1B11B996B
type: Site Annotation
comment: Transition state stabilizer
range: 22572111322429486tt112tt112

22572111322429486tt112
type: Position
type: ExactPosition
position: 112
reference: P17548-1

substrate

P17548-1
type: Simple Sequence
modified: 2009-05-05
version: 2
mass: 31589
value: MSELNESSTSKFVTINEKGLSNFRIHLNDAGQGERVIMLHGGGPGAGGWSNYYRNIGPFVEAGYRVLLPDAPGFNKSDTVVMDEQRGLVNARSVKGMMDVLGIEKAHLVGNSMGGAGALNFALEYPERTGKLILMGPGGLGNSLFTAMPMEGIKLLFKLYAEPSLETLKQMLNVFLFDQSVITDELLQGRWANIQRNPEHLKNFILSAQKVPLSAWDVSARLGEIKAKTLVTWGRDDRFVPLDHGLKLIANMQDAHVHVFPRCAHWAQWEHADAFNRLTLDFLANG

http://purl.uniprot.org/uniprot/P17548#attribution-0EFB0BE05ED7E465A7A4A1E854B43307
manual: true
evidence: ECO_0000255
source: MF_01688

http://purl.uniprot.org/uniprot/P17548#attribution-BB9CD8943AB394A5D8BF28C170F76AB5
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/2540155

http://purl.uniprot.org/uniprot/P17548#attribution-080ED3982FEC43F7C43A0689717F3D20
manual: true
evidence: ECO_0000305

A5JTM6
type: Protein
reviewed: true
created: 2010-11-30
modified: 2023-09-13
version: 45
mnemonic: CBACL_PSEUC
citation: http://purl.uniprot.org/citations/1351742
citation: SIP1B3FB6BEB7CEBA2C
citation: http://purl.uniprot.org/citations/1418673
citation: http://purl.uniprot.org/citations/1610806
citation: http://purl.uniprot.org/citations/9398293
recommended name: SIPCEBE075D2BC4DB72
enzyme: http://purl.uniprot.org/enzyme/6.2.1.33
organism: http://purl.uniprot.org/taxonomy/72586
annotation: SIPFC81CD7FA98C77F7
annotation: SIP33B1984604FDD6EC
annotation: SIP127AADDBDCD6661E
annotation: SIPB5CFF32C8E8ECE24
annotation: SIP9184E5FF7F22882B
annotation: SIPB4AA2028808A396D
annotation: SIP5491808D96A1B8F6
annotation: SIP2FB97639E52C8F7A
annotation: SIPC6441625F3912602
annotation: SIP303629B1C1FA129E
annotation: PRO_0000401169
annotation: SIP51CD200FFF629255
annotation: SIPB6E13D55301BB1FD
annotation: SIP3256A3C0AD3D10C0
annotation: SIPC3B24FA3238E3BAB
annotation: SIP678C914FD30B3D9D
annotation: SIP9D8683BD357FD0F5
annotation: SIP6796498505A936BE
annotation: SIPA272E6E200A8332D
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/67
classified with: http://purl.uniprot.org/keywords/903
classified with: http://purl.uniprot.org/keywords/436
classified with: GO_0018861
classified with: GO_0005524
sequence: A5JTM6-1

A5JTM6-citation-1351742
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

A5JTM6-citation-SIP1B3FB6BEB7CEBA2C
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

A5JTM6-citation-1418673
type: Citation Statement
scope: PROTEIN SEQUENCE OF 1-16
scope: FUNCTION
scope: CATALYTIC ACTIVITY
scope: COFACTOR
scope: ACTIVITY REGULATION
scope: BIOPHYSICOCHEMICAL PROPERTIES
scope: SUBUNIT

A5JTM6-citation-1610806
type: Citation Statement
scope: FUNCTION
scope: CATALYTIC ACTIVITY
scope: COFACTOR
scope: BIOPHYSICOCHEMICAL PROPERTIES
scope: PATHWAY
scope: SUBUNIT

A5JTM6-citation-9398293
type: Citation Statement
scope: FUNCTION
scope: CATALYTIC ACTIVITY
scope: BIOPHYSICOCHEMICAL PROPERTIES
scope: MUTAGENESIS OF GLY-163; GLY-166; PRO-168; LYS-169 AND GLU-306

cd05923
type: Resource
database: CDD
comment: CBAL
signature sequence match: CDD cd05923 match 1

IPR025110
type: Resource
database: InterPro
comment: AMP-bd_C

IPR045851
type: Resource
database: InterPro
comment: AMP-bd_C_sf

IPR020845
type: Resource
database: InterPro
comment: AMP-binding_CS

IPR000873
type: Resource
database: InterPro
comment: AMP-dep_Synth/Lig_com

PTHR43201
type: Resource
database: PANTHER
comment: ACYL-COA SYNTHETASE
signature sequence match: PANTHER PTHR43201 match 1

PTHR43201:SF8
type: Resource
database: PANTHER
comment: FATTY ACID COA SYNTHETASE FAMILY
signature sequence match: PANTHER PTHR43201:SF8 match 1

PF00501
type: Resource
database: Pfam
comment: AMP-binding
signature sequence match: Pfam PF00501 match 1

PF13193
type: Resource
database: Pfam
comment: AMP-binding_C
signature sequence match: Pfam PF13193 match 1

SSF56801
type: Resource
database: SUPFAM
comment: Acetyl-CoA synthetase-like
signature sequence match: SUPFAM SSF56801 match 1

PS00455
type: Resource
database: PROSITE
comment: AMP_BINDING
signature sequence match: PROSITE PS00455 match 1

SIPCEBE075D2BC4DB72
type: Structured Name
full name: 4-chlorobenzoate--CoA ligase
short name: 4-CBA:CoA ligase
ec name: 6.2.1.33

SIPFC81CD7FA98C77F7
type: Function Annotation
comment: Catalyzes the formation of chlorobenzoyl-CoA via a 2 step reaction. First 4-chlorobenzoyl is adenylated by ATP, followed by acyl transfer from the 4-chlorobenzoyl-AMP intermediate to CoA. Benzoate, 4-bromobenzoate, 4-iodobenzoate and 4-methylbenzoate also act as substrates. Inactive towards 4-aminobenzoate, 4-hydroxybenzoate, 2-aminobenzoate, 2,3-dihydroxybenzoate, 4-coumarate and the aliphatic carboxylic acids palmate, caproate, laurate and butyrate. Negligible activity is detected when ATP is replaced by UTP, CTP or GTP as cosubstrate.

SIPB5CFF32C8E8ECE24
type: Activity Regulation Annotation
comment: Unaffected by 5,5'-dithiobis-(2-nitrobenzoic acid), 4-chloromercuribenzoate and sodium azide. Inhibited by Cu(2+), Fe(2+) and Zn(2+). Unaffected by Na(+), K(+) and Li(+).

SIP9184E5FF7F22882B
type: Kinetics Annotation
measured affinity: 8.5 uM for 4-chlorobenzoate
measured affinity: 70 uM for CoA
measured affinity: 104 uM for ATP (with magnesium as cofactor)
measured affinity: 43 uM for ATP (with manganese as cofactor)
measured affinity: 59 uM for ATP (with cobalt as cofactor)
measured affinity: 15 uM for 4-bromobenzoate
measured affinity: 17 uM for 4-iodobenzoate
measured affinity: 700 uM for benzoate
measured affinity: 130 uM for 4-methylbenzoate

SIPB4AA2028808A396D
type: PH Dependence Annotation
comment: Optimum pH is 8.4. 85% of activity remains at pH 9.0, 54% of activity remains at pH 7.0.

SIP5491808D96A1B8F6
type: Temperature Dependence Annotation
comment: Optimum temperature is 35 degrees Celsius.

SIPC6441625F3912602
type: Subunit Annotation
comment: Homodimer.

SIP303629B1C1FA129E
type: Similarity Annotation
comment: Belongs to the ATP-dependent AMP-binding enzyme family.

PRO_0000401169
type: Chain Annotation
comment: 4-chlorobenzoate--CoA ligase
mass: 57151
range: 18354466844390958tt1tt528

18354466844390958tt1
type: Position
type: ExactPosition
position: 1
reference: A5JTM6-1

18354466844390958tt528
type: Position
type: ExactPosition
position: 528
reference: A5JTM6-1

18354466844390958tt161
type: Position
type: ExactPosition
position: 161
reference: A5JTM6-1

18354466844390958tt169
type: Position
type: ExactPosition
position: 169
reference: A5JTM6-1

18354466844390958tt300
type: Position
type: ExactPosition
position: 300
reference: A5JTM6-1

18354466844390958tt305
type: Position
type: ExactPosition
position: 305
reference: A5JTM6-1

18354466844390958tt409
type: Position
type: ExactPosition
position: 409
reference: A5JTM6-1

SIPC3B24FA3238E3BAB
type: Mutagenesis Annotation
comment: Significantly inhibits the adenylation part of the reaction.
substitution: I
range: 18354466844390958tt163tt163

18354466844390958tt163
type: Position
type: ExactPosition
position: 163
reference: A5JTM6-1

SIP678C914FD30B3D9D
type: Mutagenesis Annotation
comment: Significantly inhibits the adenylation part of the reaction.
substitution: I
range: 18354466844390958tt166tt166

18354466844390958tt166
type: Position
type: ExactPosition
position: 166
reference: A5JTM6-1

SIP9D8683BD357FD0F5
type: Mutagenesis Annotation
comment: No catalytic activity.
substitution: A
range: 18354466844390958tt168tt168

18354466844390958tt168
type: Position
type: ExactPosition
position: 168
reference: A5JTM6-1

SIP6796498505A936BE
type: Mutagenesis Annotation
comment: Significantly inhibits the adenylation part of the reaction.
substitution: M
range: 18354466844390958tt169tt169

SIPA272E6E200A8332D
type: Mutagenesis Annotation
comment: Significantly inhibits the adenylation part of the reaction.
substitution: Q
range: 18354466844390958tt306tt306

18354466844390958tt306
type: Position
type: ExactPosition
position: 306
reference: A5JTM6-1

A5JTM6-1
type: Simple Sequence
modified: 2007-06-26
version: 1
mass: 57151
value: MQTVHEMLRRAVSRVPHRWAIVDAARSTFDICRTGETSRNEGSATARLWPQPARPLAVVSGNSVEAVIAVLALHRLQAVPALMNPRLKPAEISELVARGEMARAVVANDAGVMEAIRTRVPSVCVLALDDLVSGSRVPEVAGKSLPPPPCEPEQAGFVFYTSGTTGLPKGAVIPQRAAESRVLFMATQAGLRHGSHNVVLGLMPLYHTIGFFAVLVAAMAFDGTYVVVEEFDAGNVLKLIERERVTAMFATPTHLDALTTAVEQAGARLESLEHVTFAGATMPDTVLERVNRFIPGEKVNIYGTTEAMNSLYMRAVRIAGTVMRPGFFSEVRIVRVGGDVDDGCPTVKRASWRWRRRMRPFQATLTNLRLLQKSFRKAGTGRAICVRDGSGNIVVLGRVDDMIISGGENIHPSEVERILAAAPGVAEVVVIGVKDERWGQSVVACVVLQPGASASAERLDAFCRASALADFKRPRRYVFLDELPKSAMNKVLRRQLMQHVSATSSAAVVPAPAVKQRTYAPSGRAIAR

http://purl.uniprot.org/uniprot/A5JTM6#attribution-A4F426C0AE6FF0054E952F05514A7399
creator: UniProtKB
manual: true
evidence: ECO_0000314
source: http://purl.uniprot.org/citations/1418673

http://purl.uniprot.org/uniprot/A5JTM6#attribution-609619A7DF3E53CE5E48EDEF8B8F9DD1
manual: true
evidence: ECO_0000250

http://purl.uniprot.org/uniprot/A5JTM6#attribution-63354F0DE400DC8EDCB7771F87402F6C
manual: true
evidence: ECO_0000255

http://purl.uniprot.org/uniprot/A5JTM6#attribution-0AD3424D481C7FC7123BC795FCA41ABF
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/1418673

http://purl.uniprot.org/uniprot/A5JTM6#attribution-52A33D937FBDF6FE83A720940977CC24
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/1610806

http://purl.uniprot.org/uniprot/A5JTM6#attribution-999E7165EE0C6C4F71BB4CD42EF0B2B1
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/9398293

http://purl.uniprot.org/uniprot/A5JTM6#attribution-6E0F5C938A81EDA1F76E95397D236654
manual: true
evidence: ECO_0000303
source: http://purl.uniprot.org/citations/1610806

http://purl.uniprot.org/uniprot/A5JTM6#attribution-B46D5B095E4E0B3BC450257B8FD9ED78
manual: true
evidence: ECO_0000303
source: http://purl.uniprot.org/citations/9398293

http://purl.uniprot.org/uniprot/A5JTM6#attribution-DE456E336AB38AC98E24008396E8DFDF
manual: true
evidence: ECO_0000305

http://purl.uniprot.org/uniprot/A5JTM6#attribution-259347A2937235F99FCFBDB16155273F
manual: true
evidence: ECO_0000312
source: ABQ44579.1

A5JTM5
type: Protein
reviewed: true
created: 2010-11-30
modified: 2023-09-13
version: 63
mnemonic: CBADH_PSEUC
citation: http://purl.uniprot.org/citations/1351742
citation: SIP1B3FB6BEB7CEBA2C
citation: http://purl.uniprot.org/citations/7579994
citation: http://purl.uniprot.org/citations/1610806
citation: http://purl.uniprot.org/citations/8218302
citation: http://purl.uniprot.org/citations/8718880
citation: http://purl.uniprot.org/citations/9063883
citation: http://purl.uniprot.org/citations/11747444
citation: http://purl.uniprot.org/citations/12899635
citation: http://purl.uniprot.org/citations/16388585
citation: http://purl.uniprot.org/citations/8679561
citation: http://purl.uniprot.org/citations/11695894
recommended name: SIP6E422562F2191833
enzyme: http://purl.uniprot.org/enzyme/3.8.1.7
organism: http://purl.uniprot.org/taxonomy/72586
annotation: SIP13CC26A3F42300BA
annotation: SIPA7BE788A9E15E481
annotation: SIP7947C43E6F685378
annotation: SIP144C34EEA41E261C
annotation: SIP1ABB2AB519964882
annotation: SIP471147B6B6CF310F
annotation: SIPD5A25FD0081B438E
annotation: SIP8571FEB4BF148B66
annotation: SIP609DCB093442B320
annotation: SIP42DC221D75708BC0
annotation: PRO_0000401147
annotation: SIP116AE435C5011537
annotation: SIP4EEFB2C39BAB2969
annotation: SIP72250D18A1144401
annotation: SIP9960A69EAC48615F
annotation: SIPE7471EE58645A777
annotation: SIP64238AFE02999D81
annotation: SIP45C1479F2C34131B
annotation: SIP656DCB951EFC968E
annotation: SIP3441CE34937C24BE
annotation: SIP4BEA8E3DE3E3F94E
annotation: SIPEA4DB60636EB881A
annotation: SIP6FFB876436901148
annotation: SIP7D837730B37639E6
annotation: SIPB379360C4B51478D
annotation: SIPAC4B8EB7F6193159
annotation: SIP7FCF3FE724E07A19
annotation: SIPD33F937A70B5EF21
annotation: SIP61BC28D472736170
annotation: SIPDE3185066BF78A87
annotation: SIP365BD86C72979A75
annotation: SIP0E8CEF697B4DB8C8
annotation: SIP6C74DB5C8A2A9FE6
annotation: SIPE29CCDAFF9C5D68A
annotation: SIP2EF8208755A33776
annotation: SIP6E0E79F47FC5F05F
annotation: SIP94FC8F3FA108F600
annotation: SIP8C20DF1903DCDF34
annotation: SIP07E806EEEB999D3A
annotation: SIPB3D6F502CB08744D
annotation: SIPD1C431BF7CE54D0D
annotation: SIPE142112DD8EE0E2D
annotation: SIP50F0CD968E7AFAFC
annotation: SIPAA587BC6DBA4D7C9
annotation: SIP183E206360EAF090
annotation: SIPA2B724F14F4713BC
annotation: SIPD56CA2C388E5EDA9
annotation: SIPD9103B1547679BB1
annotation: SIP6ED74020467F6DEA
annotation: SIP5314A3BDE3FD6F67
annotation: SIP40D71AED75F0057D
annotation: SIP80207CF1BCB98B4D
annotation: SIP56AA8867394630F0
annotation: SIP7B1DE0A1E776A1F6
annotation: SIPD2D45012292FA539
annotation: SIP793699EEE1F0B1E8
annotation: SIP1AA9DCE5B2E2B0BD
annotation: SIP37D8C500FCCFCC74
annotation: SIP493AE5B3EEF82348

A5JTM5-citation-1351742
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

A5JTM5-citation-SIP1B3FB6BEB7CEBA2C
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

CBS-3
type: Strain

A5JTM5-citation-7579994
type: Citation Statement
scope: PROTEIN SEQUENCE OF 1-40
scope: FUNCTION
scope: CATALYTIC ACTIVITY
scope: ACTIVITY REGULATION
scope: BIOPHYSICOCHEMICAL PROPERTIES
scope: SUBUNIT
scope: INDUCTION

A5JTM5-citation-1610806
type: Citation Statement
scope: FUNCTION
scope: CATALYTIC ACTIVITY
scope: ACTIVITY REGULATION
scope: BIOPHYSICOCHEMICAL PROPERTIES
scope: PATHWAY
scope: SUBUNIT

A5JTM5-citation-8218302
type: Citation Statement
scope: BIOPHYSICOCHEMICAL PROPERTIES

A5JTM5-citation-8718880
type: Citation Statement
scope: ACTIVITY REGULATION
scope: ACTIVE SITES
scope: MUTAGENESIS OF GLU-34; GLU-43; ASP-45; ASP-46; GLU-68; HIS-90; HIS-94; ASP-123; ASP-129; TRP-137; GLU-163; GLU-175; TRP-179 AND HIS-208

A5JTM5-citation-9063883
type: Citation Statement
scope: BIOPHYSICOCHEMICAL PROPERTIES
scope: MUTAGENESIS OF PHE-64; HIS-81; PHE-82; TRP-89; HIS-90; GLY-114; TRP-137 AND ASP-145

A5JTM5-citation-11747444
type: Citation Statement
scope: BIOPHYSICOCHEMICAL PROPERTIES
scope: MUTAGENESIS OF ARG-24; PHE-64; ARG-67; GLY-113; GLY-114 AND ARG-257

A5JTM5-citation-12899635
type: Citation Statement
scope: BIOPHYSICOCHEMICAL PROPERTIES
scope: MUTAGENESIS OF GLY-63; PHE-64; TYR-65; ALA-112; GLY-113 AND GLY-115

A5JTM5-citation-16388585
type: Citation Statement
scope: BIOPHYSICOCHEMICAL PROPERTIES
scope: MUTAGENESIS OF ARG-216 AND GLU-232

A5JTM5-citation-8679561
type: Citation Statement
scope: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) OF MUTANT GLN-90 IN COMPLEX WITH 4-HYDROXYBENZOYL COENZYME A
scope: SUBUNIT
scope: ACTIVE SITE
scope: SUBSTRATE-BINDING SITES

A5JTM5-citation-11695894
type: Citation Statement
scope: X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) OF MUTANT GLN-90 IN COMPLEX WITH 4-HYDROXYBENZOYL COENZYME A
scope: BIOPHYSICOCHEMICAL PROPERTIES
scope: MUTAGENESIS OF HIS-90

1JXZ
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 1.90
chain sequence mapping: PDB 1JXZ tt1tt269

PDB 1JXZ tt1tt269
chain: A/B/C=1-269

1NZY
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 1.80
chain sequence mapping: PDB 1NZY tt1tt269

PDB 1NZY tt1tt269
chain: A/B/C=1-269

http://purl.uniprot.org/brenda/3.8.1.7
type: Resource
database: BRENDA
comment: 5085

cd06558
type: Resource
database: CDD
comment: crotonase-like
signature sequence match: CDD cd06558 match 1

http://purl.uniprot.org/gene3d/1.10.12.10
type: Resource
database: Gene3D
comment: Lyase 2-enoyl-coa Hydratase, Chain A, domain 2
signature sequence match: Gene3D 1.10.12.10 match 1

IPR029045
type: Resource
database: InterPro
comment: ClpP/crotonase-like_dom_sf

IPR018376
type: Resource
database: InterPro
comment: Enoyl-CoA_hyd/isom_CS

IPR001753
type: Resource
database: InterPro
comment: Enoyl-CoA_hydra/iso

IPR014748
type: Resource
database: InterPro
comment: Enoyl-CoA_hydra_C

PTHR43684:SF4
type: Resource
database: PANTHER
comment: ENOYL-COA HYDRATASE/ISOMERASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_1G01890)
signature sequence match: PANTHER PTHR43684:SF4 match 1

PF00378
type: Resource
database: Pfam
comment: ECH_1
signature sequence match: Pfam PF00378 match 1

SSF52096
type: Resource
database: SUPFAM
comment: ClpP/crotonase
signature sequence match: SUPFAM SSF52096 match 1

PS00166
type: Resource
database: PROSITE
comment: ENOYL_COA_HYDRATASE
signature sequence match: PROSITE PS00166 match 1

SIP6E422562F2191833
type: Structured Name
full name: 4-chlorobenzoyl coenzyme A dehalogenase
short name: 4-CBA-CoA dehalogenase
short name: 4-CBCoA dehalogenase
short name: 4-chlorobenzoyl-CoA dehalogenase
ec name: 3.8.1.7

SIP13CC26A3F42300BA
type: Function Annotation
comment: Dehalogenates 4-chlorobenzoyl-CoA, 4-iodobenzoyl-CoA and 4-bromobenzoyl-CoA, but not 4-fluorobenzoyl-CoA. Inactive towards crotonyl-CoA, alpha-methylcrotonyl-CoA and beta-methylcrotonyl-CoA.

SIP7947C43E6F685378
type: Activity Regulation Annotation
comment: Inactivated by 1 mM Ag(+) and by 5 mM Cu(2+). Partially inhibited by 5 mM Zn(2+), Mn(2+), Co(2+), Fe(2+) and Ni(2+). Unaffected by 10 mM Na(+), K(+) and Li(+) and by 0.5 mM Mg(2+), Mn(2+), Fe(2+), Ca(2+), Co(2+) and Zn(2+). Inhibited by the sulfhydryl blocking agent 5,5'-dithio-bis-(2-nitrobenzoate), SDS and N-bromosuccinimide. Unaffected by sodium azide and EDTA. Inactivated following treatment with diethyl pyrocarbonate; this inactivation is reversible by treatment with hydroxylamine.

SIP144C34EEA41E261C
type: Kinetics Annotation
measured affinity: 3.7 uM for 4-chlorobenzoyl-CoA
measured affinity: 4.2 uM for 4-bromobenzoyl-CoA
measured affinity: 6.5 uM for 4-iodobenzoyl-CoA
measured affinity: 10.4 uM for 2,4-dichlorobenzoyl-CoA
measured affinity: 42 uM for 3,4-dichlorobenzoyl-CoA
measured affinity: 30 uM for 4-chloro-2-nitrobenzoyl-CoA
measured affinity: 5.5 uM for 4-chloro-3-nitrobenzoyl-CoA
measured activity: 2.2 umol/min/mg enzyme with 4-chlorobenzoyl-CoA as substrate

SIP1ABB2AB519964882
type: PH Dependence Annotation
comment: Optimum pH is 10.0. Half maximum activity remains at pH 9.0 and 11.5. Stable from pH 6.5 to 11.0, activity is lost following 10 minutes incubation at pH 4.5 or 12.0.

SIP471147B6B6CF310F
type: Temperature Dependence Annotation
comment: Optimum temperature is 60 degrees Celsius. Retains 60% of maximum activity at 30 degrees Celsius and 65 degrees Celsius. After 15 minutes preincubation at 60 degrees Celsius 70% of the initial activity remains, 15 minutes preincubation at 65 degrees Celsius results in a total loss of activity.

SIP8571FEB4BF148B66
type: Subunit Annotation
comment: Homotetramer (PubMed:1610806). Homotetramer, homooctamer and larger multimers (PubMed:7579994). Homotrimer (PubMed:8679561).

SIP609DCB093442B320
type: Induction Annotation
comment: By 4-chlorobenzoyl-CoA, 4-iodobenzoyl-CoA or 4-bromobenzoyl-CoA.

SIP42DC221D75708BC0
type: Similarity Annotation
comment: Belongs to the enoyl-CoA hydratase/isomerase family.

PRO_0000401147
type: Chain Annotation
comment: 4-chlorobenzoyl coenzyme A dehalogenase
mass: 29802
range: 18354466844390702tt1tt269

18354466844390702tt1
type: Position
type: ExactPosition
position: 1
reference: A5JTM5-1

18354466844390702tt269
type: Position
type: ExactPosition
position: 269
reference: A5JTM5-1

SIP116AE435C5011537
type: Active Site Annotation
comment: Proton acceptor
range: 18354466844390702tt90tt90

18354466844390702tt90
type: Position
type: ExactPosition
position: 90
reference: A5JTM5-1

SIP4EEFB2C39BAB2969
type: Active Site Annotation
comment: Nucleophile
range: 18354466844390702tt145tt145

18354466844390702tt145
type: Position
type: ExactPosition
position: 145
reference: A5JTM5-1

SIP72250D18A1144401
type: Binding Site Annotation
comment: in other chain
ligand: SIP11C6EFF6FEA76027
range: 18354466844390702tt24tt24

18354466844390702tt24
type: Position
type: ExactPosition
position: 24
reference: A5JTM5-1

SIP9960A69EAC48615F
type: Binding Site Annotation
comment: in other chain
ligand: SIP11C6EFF6FEA76027
range: 18354466844390702tt62tt67

18354466844390702tt62
type: Position
type: ExactPosition
position: 62
reference: A5JTM5-1

18354466844390702tt67
type: Position
type: ExactPosition
position: 67
reference: A5JTM5-1

SIPE7471EE58645A777
type: Binding Site Annotation
comment: in other chain
ligand: SIP11C6EFF6FEA76027
range: 18354466844390702tt114tt114

18354466844390702tt114
type: Position
type: ExactPosition
position: 114
reference: A5JTM5-1

18354466844390702tt257
type: Position
type: ExactPosition
position: 257
reference: A5JTM5-1

SIP45C1479F2C34131B
type: Mutagenesis Annotation
comment: Does not strongly affect catalytic activity, but reduces substrate CoA binding.
substitution: K
substitution: L
range: 18354466844390702tt24tt24

SIP656DCB951EFC968E
type: Mutagenesis Annotation
comment: Forms inclusion bodies.
substitution: T
range: 18354466844390702tt34tt34

18354466844390702tt34
type: Position
type: ExactPosition
position: 34
reference: A5JTM5-1

SIP3441CE34937C24BE
type: Mutagenesis Annotation
comment: No effect on catalytic activity.
substitution: A
range: 18354466844390702tt43tt43

18354466844390702tt43
type: Position
type: ExactPosition
position: 43
reference: A5JTM5-1

SIP4BEA8E3DE3E3F94E
type: Mutagenesis Annotation
comment: No effect on catalytic activity.
substitution: A
range: 18354466844390702tt45tt45

18354466844390702tt45
type: Position
type: ExactPosition
position: 45
reference: A5JTM5-1

SIPEA4DB60636EB881A
type: Mutagenesis Annotation
comment: No effect on catalytic activity.
substitution: A
range: 18354466844390702tt46tt46

18354466844390702tt46
type: Position
type: ExactPosition
position: 46
reference: A5JTM5-1

SIP6FFB876436901148
type: Mutagenesis Annotation
comment: Yields insoluble protein.
substitution: A
substitution: I
substitution: P
range: 18354466844390702tt63tt63

18354466844390702tt63
type: Position
type: ExactPosition
position: 63
reference: A5JTM5-1

SIP7D837730B37639E6
type: Mutagenesis Annotation
comment: 30-fold reduction in catalytic activity, substrate benzoyl group binding is unaffected.
substitution: A
range: 18354466844390702tt64tt64

18354466844390702tt64
type: Position
type: ExactPosition
position: 64
reference: A5JTM5-1

SIPB379360C4B51478D
type: Mutagenesis Annotation
comment: Retains catalytic activity, but substrate benzoyl group binding is decreased.
substitution: L
range: 18354466844390702tt64tt64

SIPAC4B8EB7F6193159
type: Mutagenesis Annotation
comment: Severely reduces catalytic activity. Arylated intermediate does not accumulate.
substitution: P
range: 18354466844390702tt64tt64

SIP7FCF3FE724E07A19
type: Mutagenesis Annotation
comment: Catalytic activity is almost as efficient as wild type.
substitution: D
range: 18354466844390702tt65tt65

18354466844390702tt65
type: Position
type: ExactPosition
position: 65
reference: A5JTM5-1

SIPD33F937A70B5EF21
type: Mutagenesis Annotation
comment: Reduces substrate CoA binding.
substitution: K
range: 18354466844390702tt67tt67

SIP61BC28D472736170
type: Mutagenesis Annotation
comment: Forms inclusion bodies.
substitution: L
range: 18354466844390702tt67tt67

SIPDE3185066BF78A87
type: Mutagenesis Annotation
comment: No effect on catalytic activity.
substitution: T
range: 18354466844390702tt68tt68

18354466844390702tt68
type: Position
type: ExactPosition
position: 68
reference: A5JTM5-1

SIP365BD86C72979A75
type: Mutagenesis Annotation
comment: Loss of catalytic activity, substrate benzoyl group binding is not affected.
substitution: Q
range: 18354466844390702tt81tt81

18354466844390702tt81
type: Position
type: ExactPosition
position: 81
reference: A5JTM5-1

SIP0E8CEF697B4DB8C8
type: Mutagenesis Annotation
comment: Retains catalytic activity, but substrate benzoyl group binding is decreased.
substitution: L
range: 18354466844390702tt82tt82

18354466844390702tt82
type: Position
type: ExactPosition
position: 82
reference: A5JTM5-1

SIP6C74DB5C8A2A9FE6
type: Mutagenesis Annotation
comment: Retains catalytic activity, but substrate benzoyl group binding is decreased.
substitution: F
range: 18354466844390702tt89tt89

18354466844390702tt89
type: Position
type: ExactPosition
position: 89
reference: A5JTM5-1

SIPE29CCDAFF9C5D68A
type: Mutagenesis Annotation
comment: Reduced activity and substrate benzoyl group binding.
substitution: Y
range: 18354466844390702tt89tt89

SIP2EF8208755A33776
type: Mutagenesis Annotation
comment: Complete loss of catalytic activity (PubMed:8718880, PubMed:9063883). Significantly reduced activity (PubMed:11695894). Substrate binding is not significantly affected. Reduced arylated intermediate formation.
substitution: Q
range: 18354466844390702tt90tt90

SIP6E0E79F47FC5F05F
type: Mutagenesis Annotation
comment: No effect on catalytic activity.
substitution: Q
range: 18354466844390702tt94tt94

18354466844390702tt94
type: Position
type: ExactPosition
position: 94
reference: A5JTM5-1

SIP94FC8F3FA108F600
type: Mutagenesis Annotation
comment: Yields insoluble protein.
substitution: G
range: 18354466844390702tt112tt112

18354466844390702tt112
type: Position
type: ExactPosition
position: 112
reference: A5JTM5-1

SIP8C20DF1903DCDF34
type: Mutagenesis Annotation
comment: Protein precipitates upon purification.
substitution: S
range: 18354466844390702tt112tt112

SIP07E806EEEB999D3A
type: Mutagenesis Annotation
comment: Catalytic activity is almost as efficient as wild type.
substitution: V
range: 18354466844390702tt112tt112

SIPB3D6F502CB08744D
type: Mutagenesis Annotation
comment: Strongly reduced catalytic activity and substrate benzoyl group binding. Arylated intermediate does not accumulate.
substitution: A
range: 18354466844390702tt113tt113

18354466844390702tt113
type: Position
type: ExactPosition
position: 113
reference: A5JTM5-1

SIPD1C431BF7CE54D0D
type: Mutagenesis Annotation
comment: Strongly reduced catalytic activity. Arylated intermediate does not accumulate.
substitution: N
substitution: S
range: 18354466844390702tt113tt113

SIPE142112DD8EE0E2D
type: Mutagenesis Annotation
comment: Protein precipitates upon purification.
substitution: V
range: 18354466844390702tt113tt113

SIP50F0CD968E7AFAFC
type: Mutagenesis Annotation
comment: Strongly reduced catalytic activity and substrate benzoyl group binding.
substitution: A
range: 18354466844390702tt114tt114

SIPAA587BC6DBA4D7C9
type: Mutagenesis Annotation
comment: Unstable.
substitution: P
range: 18354466844390702tt114tt114

SIP183E206360EAF090
type: Mutagenesis Annotation
comment: Yields insoluble protein.
substitution: L
substitution: N
substitution: S
substitution: V
range: 18354466844390702tt115tt115

18354466844390702tt115
type: Position
type: ExactPosition
position: 115
reference: A5JTM5-1

SIPA2B724F14F4713BC
type: Mutagenesis Annotation
comment: No effect on catalytic activity.
substitution: T
range: 18354466844390702tt123tt123

18354466844390702tt123
type: Position
type: ExactPosition
position: 123
reference: A5JTM5-1

SIPD56CA2C388E5EDA9
type: Mutagenesis Annotation
comment: No effect on catalytic activity.
substitution: T
range: 18354466844390702tt129tt129

18354466844390702tt129
type: Position
type: ExactPosition
position: 129
reference: A5JTM5-1

SIPD9103B1547679BB1
type: Mutagenesis Annotation
comment: Low catalytic activity, but KM unaffected (PubMed:8718880). Retains catalytic activity, but substrate benzoyl group binding is decreased (PubMed:9063883).
substitution: F
range: 18354466844390702tt137tt137

18354466844390702tt137
type: Position
type: ExactPosition
position: 137
reference: A5JTM5-1

SIP6ED74020467F6DEA
type: Mutagenesis Annotation
comment: Complete loss of catalytic activity, but not substrate binding.
substitution: A
range: 18354466844390702tt145tt145

SIP5314A3BDE3FD6F67
type: Mutagenesis Annotation
comment: No effect on catalytic activity.
substitution: T
range: 18354466844390702tt163tt163

18354466844390702tt163
type: Position
type: ExactPosition
position: 163
reference: A5JTM5-1

SIP40D71AED75F0057D
type: Mutagenesis Annotation
comment: No effect on catalytic activity.
substitution: D
range: 18354466844390702tt175tt175

18354466844390702tt175
type: Position
type: ExactPosition
position: 175
reference: A5JTM5-1

SIP80207CF1BCB98B4D
type: Mutagenesis Annotation
comment: No effect on catalytic activity.
substitution: F
range: 18354466844390702tt179tt179

18354466844390702tt179
type: Position
type: ExactPosition
position: 179
reference: A5JTM5-1

SIP56AA8867394630F0
type: Mutagenesis Annotation
comment: No effect on catalytic activity.
substitution: Q
range: 18354466844390702tt208tt208

18354466844390702tt208
type: Position
type: ExactPosition
position: 208
reference: A5JTM5-1

SIP7B1DE0A1E776A1F6
type: Mutagenesis Annotation
comment: Yields insoluble protein.
substitution: E
substitution: K
substitution: L
range: 18354466844390702tt216tt216

18354466844390702tt216
type: Position
type: ExactPosition
position: 216
reference: A5JTM5-1

SIPD2D45012292FA539
type: Mutagenesis Annotation
comment: Yields insoluble protein.
substitution: A
substitution: N
substitution: Q
substitution: R
range: 18354466844390702tt232tt232

18354466844390702tt232
type: Position
type: ExactPosition
position: 232
reference: A5JTM5-1

SIP793699EEE1F0B1E8
type: Mutagenesis Annotation
comment: Reduced catalytic activity, increased substrate binding.
substitution: D
range: 18354466844390702tt232tt232

SIP1AA9DCE5B2E2B0BD
type: Mutagenesis Annotation
comment: Retains catalytic activity and substrate CoA binding.
substitution: K
range: 18354466844390702tt257tt257

SIP37D8C500FCCFCC74
type: Mutagenesis Annotation
comment: Significantly reduces catalytic activity and substrate CoA binding.
substitution: L
range: 18354466844390702tt257tt257

18354466844390702tt3
type: Position
type: ExactPosition
position: 3
reference: A5JTM5-1

18354466844390702tt10
type: Position
type: ExactPosition
position: 10
reference: A5JTM5-1

18354466844390702tt13
type: Position
type: ExactPosition
position: 13
reference: A5JTM5-1

18354466844390702tt18
type: Position
type: ExactPosition
position: 18
reference: A5JTM5-1

18354466844390702tt21
type: Position
type: ExactPosition
position: 21
reference: A5JTM5-1

18354466844390702tt23
type: Position
type: ExactPosition
position: 23
reference: A5JTM5-1

18354466844390702tt29
type: Position
type: ExactPosition
position: 29
reference: A5JTM5-1

18354466844390702tt44
type: Position
type: ExactPosition
position: 44
reference: A5JTM5-1

18354466844390702tt50
type: Position
type: ExactPosition
position: 50
reference: A5JTM5-1

18354466844390702tt56
type: Position
type: ExactPosition
position: 56
reference: A5JTM5-1

18354466844390702tt59
type: Position
type: ExactPosition
position: 59
reference: A5JTM5-1

18354466844390702tt61
type: Position
type: ExactPosition
position: 61
reference: A5JTM5-1

18354466844390702tt66
type: Position
type: ExactPosition
position: 66
reference: A5JTM5-1

18354466844390702tt71
type: Position
type: ExactPosition
position: 71
reference: A5JTM5-1

18354466844390702tt73
type: Position
type: ExactPosition
position: 73
reference: A5JTM5-1

18354466844390702tt74
type: Position
type: ExactPosition
position: 74
reference: A5JTM5-1

18354466844390702tt98
type: Position
type: ExactPosition
position: 98
reference: A5JTM5-1

18354466844390702tt99
type: Position
type: ExactPosition
position: 99
reference: A5JTM5-1

18354466844390702tt101
type: Position
type: ExactPosition
position: 101
reference: A5JTM5-1

18354466844390702tt103
type: Position
type: ExactPosition
position: 103
reference: A5JTM5-1

18354466844390702tt107
type: Position
type: ExactPosition
position: 107
reference: A5JTM5-1

18354466844390702tt109
type: Position
type: ExactPosition
position: 109
reference: A5JTM5-1

18354466844390702tt121
type: Position
type: ExactPosition
position: 121
reference: A5JTM5-1

18354466844390702tt122
type: Position
type: ExactPosition
position: 122
reference: A5JTM5-1

18354466844390702tt128
type: Position
type: ExactPosition
position: 128
reference: A5JTM5-1

18354466844390702tt132
type: Position
type: ExactPosition
position: 132
reference: A5JTM5-1

18354466844390702tt134
type: Position
type: ExactPosition
position: 134
reference: A5JTM5-1

18354466844390702tt140
type: Position
type: ExactPosition
position: 140
reference: A5JTM5-1

18354466844390702tt148
type: Position
type: ExactPosition
position: 148
reference: A5JTM5-1

18354466844390702tt167
type: Position
type: ExactPosition
position: 167
reference: A5JTM5-1

18354466844390702tt173
type: Position
type: ExactPosition
position: 173
reference: A5JTM5-1

18354466844390702tt178
type: Position
type: ExactPosition
position: 178
reference: A5JTM5-1

18354466844390702tt183
type: Position
type: ExactPosition
position: 183
reference: A5JTM5-1

18354466844390702tt186
type: Position
type: ExactPosition
position: 186
reference: A5JTM5-1

18354466844390702tt188
type: Position
type: ExactPosition
position: 188
reference: A5JTM5-1

18354466844390702tt204
type: Position
type: ExactPosition
position: 204
reference: A5JTM5-1

18354466844390702tt207
type: Position
type: ExactPosition
position: 207
reference: A5JTM5-1

18354466844390702tt220
type: Position
type: ExactPosition
position: 220
reference: A5JTM5-1

18354466844390702tt225
type: Position
type: ExactPosition
position: 225
reference: A5JTM5-1

18354466844390702tt241
type: Position
type: ExactPosition
position: 241
reference: A5JTM5-1

18354466844390702tt245
type: Position
type: ExactPosition
position: 245
reference: A5JTM5-1

18354466844390702tt253
type: Position
type: ExactPosition
position: 253
reference: A5JTM5-1

SIP11C6EFF6FEA76027
subclass of: substrate
comment: ligand shared between two oligomeric partners

A5JTM5-1
type: Simple Sequence
modified: 2007-06-26
version: 1
mass: 29802
value: MYEAIGHRVEDGVAEITIKLPRHRNALSVKAMQEVTDALNRAEEDDSVGAVMITGAEDAFCAGFYLREIPLDKGVAGVRDHFRIGALWWHQMIHKIIRVKRPVLAAINGVAAGGGLGISLASDMAICADSAKFVCAWHTIGIGNDTATSYSLARIVGMRRAMELMLTNRTLYPEEAKDWGLVSRVYPKDDFREVAWKVARELAAAPTHLQVMAKERFHAGWMQPVEECTEFEIQNVIASVTHPHFMPCLTEFLDGHRADRPQVELPAGV

http://purl.uniprot.org/uniprot/A5JTM5#attribution-CE2051493E3509DECD79F5306DBA6848
creator: UniProtKB
manual: true
evidence: ECO_0000314
source: http://purl.uniprot.org/citations/7579994

http://purl.uniprot.org/uniprot/A5JTM5#attribution-9BD82EDBA11B5738907FB4A9D8E9030E
manual: true
evidence: ECO_0000250
source: O85078

http://purl.uniprot.org/uniprot/A5JTM5#attribution-3A2B987566E213BD3BB7A62C079D4161
manual: true
evidence: ECO_0000255

http://purl.uniprot.org/uniprot/A5JTM5#attribution-45AA31476E7C7ED913A4042E6E389599
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/11695894

http://purl.uniprot.org/uniprot/A5JTM5#attribution-5DC17EBCAA748026D665FB659821E408
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/11747444

http://purl.uniprot.org/uniprot/A5JTM5#attribution-0E6E6D2D16F91D01BFCDE8F824FF9B2B
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/12899635

http://purl.uniprot.org/uniprot/A5JTM5#attribution-261DE5EDBF225C49BD16BC0CD1196E5E
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/1610806

http://purl.uniprot.org/uniprot/A5JTM5#attribution-6596C1435CAC61706FB12DABABDE67D9
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/16388585

http://purl.uniprot.org/uniprot/A5JTM5#attribution-469AE044B87CC99AFBA1B05092096048
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/7579994

http://purl.uniprot.org/uniprot/A5JTM5#attribution-F0CE7A4811D90900D8FA409E3E333470
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/8218302

http://purl.uniprot.org/uniprot/A5JTM5#attribution-C3026F639E55D772E54573E93D2C060D
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/8679561

http://purl.uniprot.org/uniprot/A5JTM5#attribution-A27300A0A411DC55290098FDCA48A4DC
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/8718880

http://purl.uniprot.org/uniprot/A5JTM5#attribution-184F0D2ADFF96FB359B8A514A69FB5FE
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/9063883

http://purl.uniprot.org/uniprot/A5JTM5#attribution-291C707A01E6FC55ACD081F9A059DFA0
manual: true
evidence: ECO_0000303
source: http://purl.uniprot.org/citations/1610806

http://purl.uniprot.org/uniprot/A5JTM5#attribution-726E62FBCE8F6F7C353F0EFE25D395D4
manual: true
evidence: ECO_0000303
source: http://purl.uniprot.org/citations/7579994

http://purl.uniprot.org/uniprot/A5JTM5#attribution-2516CBE0764E2D2D5023E3EC6AE5E7F5
manual: true
evidence: ECO_0000305

http://purl.uniprot.org/uniprot/A5JTM5#attribution-09FCD8A7145D57E4697C8B84F75214AE
manual: true
evidence: ECO_0000312
source: ABQ44578.1

P06621
type: Protein
reviewed: true
created: 1988-01-01
modified: 2023-05-03
version: 112
mnemonic: CBPG_PSES6
old mnemonic: CBPG_PSESP
citation: http://purl.uniprot.org/citations/6396165
citation: SIPC6E659A3A04F6A95
citation: http://purl.uniprot.org/citations/3838935
citation: http://purl.uniprot.org/citations/9083113
recommended name: SIPBB444374D5C7FFD5
alternative name: SIPEF642D078F43944A
alternative name: SIP2958E8CB51B35344
alternative name: SIP1499401E81109998
alternative name: SIPEB79CC1D3EC41D5E
enzyme: http://purl.uniprot.org/enzyme/3.4.17.11
organism: http://purl.uniprot.org/taxonomy/312
annotation: SIPD273515959700130
annotation: SIP30F0E7AA95608AE0
annotation: SIP4E0C717A9D0CD62D
annotation: SIP2496A67AF46A58E6
annotation: SIP6C3EA9352DACE190
annotation: SIPFF4F8F0BD0032EE4
annotation: SIP8B75ABBC1330277C
annotation: PRO_0000026808
annotation: SIP0D75CD1DB3338851
annotation: SIP7D33E3EF8CFB3D95
annotation: SIPC6B4E4F4572E8F08
annotation: SIP1B83275DDDA4C03C
annotation: SIP3AFBED2B897DC412
annotation: SIPDC0758F3EDF01533
annotation: SIP9252731284D9112D
annotation: SIPB55AA4DB8BD490AA
annotation: SIP4ED43848769F99E5
annotation: SIP0194D7ADFCDEC0BB
annotation: SIP7659B576B1873ADC
annotation: SIPD7E2EC991A09705B
annotation: SIP3D8EAB0AFA2979B7
annotation: SIPC7E12D7EACCBAB2E
annotation: SIP26E243AE1EC7A032
annotation: SIPA710792BD7C77827
annotation: SIP0CC58D45A14D4363
annotation: SIP4CC703E661F18795
annotation: SIP069B370002F6958C
annotation: SIP111C8251FD64E5A1
annotation: SIPE271C71F43AE3781
annotation: SIPBF98B13C060C5A33
annotation: SIP8DFD8183A71BE4C0
annotation: SIPF874AD01715E2231
annotation: SIPB258B0913C4EA772
annotation: SIP4C536A2CB0F2CF86
annotation: SIPC397C870D8871347
annotation: SIP1F920B3FFD254218
annotation: SIPE38D3EABF63BDABB
annotation: SIPD44EB46338BC56AD
annotation: SIP508F9216FF8687D7
annotation: SIP5DE150079FB788B1
annotation: SIP2CACE70A8941D16C
annotation: SIP0B9AAC7D8C885F48
annotation: SIP7C88F4D792225357
annotation: SIP1F4829D90A705466
annotation: SIP4F7F3BFDD4FF4EAB
annotation: SIP729B4B503DAA8159
annotation: SIP570D534646A6F3B7
annotation: SIP9C3F9FC5D60CA2C8
annotation: SIP3E83BFA3F7E0BF89
annotation: SIP92BB14982DDDD3FE
annotation: SIPE9BA99034F96EB71
annotation: SIP58019CE6374CD943
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/2
classified with: http://purl.uniprot.org/keywords/121
classified with: http://purl.uniprot.org/keywords/903
classified with: http://purl.uniprot.org/keywords/479
classified with: http://purl.uniprot.org/keywords/482
classified with: http://purl.uniprot.org/keywords/582
classified with: http://purl.uniprot.org/keywords/732
classified with: http://purl.uniprot.org/keywords/862
classified with: GO_0004180

P06621-citation-6396165
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
scope: PARTIAL PROTEIN SEQUENCE

P06621-citation-SIPC6E659A3A04F6A95
type: Citation Statement
scope: ERRATUM OF PUBMED:6396165

P06621-citation-3838935
type: Citation Statement
scope: CHARACTERIZATION

P06621-citation-9083113
type: Citation Statement
scope: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 23-415 IN COMPLEX WITH ZINC
scope: COFACTOR
scope: ACTIVE SITE

A24955
type: Resource
database: PIR
comment: YXPSF2

1CG2
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.50
chain sequence mapping: PDB 1CG2 tt23tt415

PDB 1CG2 tt23tt415
chain: A/B/C/D=23-415

6XJ5
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 3.11
chain sequence mapping: PDB 6XJ5 tt24tt415

PDB 6XJ5 tt24tt415
chain: A/B/C/D/E/F/G/H=24-415

7M6U
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.59
chain sequence mapping: PDB 7M6U tt25tt415

PDB 7M6U tt25tt415
chain: A/B/C/D=25-415

DB00563
type: Resource
database: DrugBank
comment: Methotrexate

http://purl.uniprot.org/brenda/3.4.17.11
type: Resource
database: BRENDA
comment: 5085

cd03885
type: Resource
database: CDD
comment: M20_CPDG2
signature sequence match: CDD cd03885 match 1

http://purl.uniprot.org/gene3d/3.40.630.10
type: Resource
database: Gene3D
comment: Zn peptidases
signature sequence match: Gene3D 3.40.630.10 match 1

IPR001261
type: Resource
database: InterPro
comment: ArgE/DapE_CS

IPR036264
type: Resource
database: InterPro
comment: Bact_exopeptidase_dim_dom

IPR017150
type: Resource
database: InterPro
comment: Pept_M20_glutamate_carboxypep

IPR002933
type: Resource
database: InterPro
comment: Peptidase_M20

IPR011650
type: Resource
database: InterPro
comment: Peptidase_M20_dimer

PTHR43808
type: Resource
database: PANTHER
comment: ACETYLORNITHINE DEACETYLASE
signature sequence match: PANTHER PTHR43808 match 1

PTHR43808:SF10
type: Resource
database: PANTHER
comment: BLL3749 PROTEIN
signature sequence match: PANTHER PTHR43808:SF10 match 1

PF07687
type: Resource
database: Pfam
comment: M20_dimer
signature sequence match: Pfam PF07687 match 1

PF01546
type: Resource
database: Pfam
comment: Peptidase_M20
signature sequence match: Pfam PF01546 match 1

PIRSF037238
type: Resource
database: PIRSF
comment: Carboxypeptidase_G2
signature sequence match: PIRSF PIRSF037238 match 1

SSF55031
type: Resource
database: SUPFAM
comment: Bacterial exopeptidase dimerisation domain
signature sequence match: SUPFAM SSF55031 match 1

SSF53187
type: Resource
database: SUPFAM
comment: Zn-dependent exopeptidases
signature sequence match: SUPFAM SSF53187 match 1

PS00758
type: Resource
database: PROSITE
comment: ARGE_DAPE_CPG2_1
signature sequence match: PROSITE PS00758 match 1

PS00759
type: Resource
database: PROSITE
comment: ARGE_DAPE_CPG2_2
signature sequence match: PROSITE PS00759 match 1

SIPBB444374D5C7FFD5
type: Structured Name
full name: Carboxypeptidase G2
short name: CPDG2
ec name: 3.4.17.11

SIPEF642D078F43944A
type: Structured Name
full name: Folate hydrolase G2

SIP2958E8CB51B35344
type: Structured Name
full name: Glutamate carboxypeptidase

SIP1499401E81109998
type: Structured Name
full name: Pteroylmonoglutamic acid hydrolase G2

SIPEB79CC1D3EC41D5E
type: Structured Name
international nonproprietary name: Glucarpidase

cpg2
type: Gene

SIPD273515959700130
type: Function Annotation
comment: Catalyzes the hydrolysis of reduced and non-reduced folates to pteroates and L-glutamate. This enzyme has a broad specificity.

SIP4E0C717A9D0CD62D
type: Cofactor Annotation
comment: Binds 2 Zn(2+) ions per subunit.
cofactor: CHEBI_29105

SIP2496A67AF46A58E6
type: Subunit Annotation
comment: Homodimer.

SIP6C3EA9352DACE190
type: Pharmaceutical Annotation
comment: Used clinically as a folate-depleting, antitumor agent.

SIPFF4F8F0BD0032EE4
type: Similarity Annotation
comment: Belongs to the peptidase M20A family.

22571007532478766tt1
type: Position
type: ExactPosition
position: 1
reference: P06621-1

22571007532478766tt22
type: Position
type: ExactPosition
position: 22
reference: P06621-1

PRO_0000026808
type: Chain Annotation
comment: Carboxypeptidase G2
mass: 41695
range: 22571007532478766tt23tt415

22571007532478766tt23
type: Position
type: ExactPosition
position: 23
reference: P06621-1

22571007532478766tt415
type: Position
type: ExactPosition
position: 415
reference: P06621-1

22571007532478766tt114
type: Position
type: ExactPosition
position: 114
reference: P06621-1

SIP7D33E3EF8CFB3D95
type: Active Site Annotation
comment: Proton acceptor
range: 22571007532478766tt175tt175

22571007532478766tt175
type: Position
type: ExactPosition
position: 175
reference: P06621-1

22571007532478766tt112
type: Position
type: ExactPosition
position: 112
reference: P06621-1

22571007532478766tt141
type: Position
type: ExactPosition
position: 141
reference: P06621-1

22571007532478766tt176
type: Position
type: ExactPosition
position: 176
reference: P06621-1

22571007532478766tt200
type: Position
type: ExactPosition
position: 200
reference: P06621-1

22571007532478766tt385
type: Position
type: ExactPosition
position: 385
reference: P06621-1

22571007532478766tt30
type: Position
type: ExactPosition
position: 30
reference: P06621-1

22571007532478766tt51
type: Position
type: ExactPosition
position: 51
reference: P06621-1

22571007532478766tt60
type: Position
type: ExactPosition
position: 60
reference: P06621-1

22571007532478766tt76
type: Position
type: ExactPosition
position: 76
reference: P06621-1

22571007532478766tt80
type: Position
type: ExactPosition
position: 80
reference: P06621-1

22571007532478766tt85
type: Position
type: ExactPosition
position: 85
reference: P06621-1

22571007532478766tt92
type: Position
type: ExactPosition
position: 92
reference: P06621-1

22571007532478766tt100
type: Position
type: ExactPosition
position: 100
reference: P06621-1

22571007532478766tt107
type: Position
type: ExactPosition
position: 107
reference: P06621-1

22571007532478766tt121
type: Position
type: ExactPosition
position: 121
reference: P06621-1

22571007532478766tt124
type: Position
type: ExactPosition
position: 124
reference: P06621-1

22571007532478766tt128
type: Position
type: ExactPosition
position: 128
reference: P06621-1

22571007532478766tt130
type: Position
type: ExactPosition
position: 130
reference: P06621-1

22571007532478766tt133
type: Position
type: ExactPosition
position: 133
reference: P06621-1

22571007532478766tt135
type: Position
type: ExactPosition
position: 135
reference: P06621-1

22571007532478766tt137
type: Position
type: ExactPosition
position: 137
reference: P06621-1

22571007532478766tt142
type: Position
type: ExactPosition
position: 142
reference: P06621-1

22571007532478766tt143
type: Position
type: ExactPosition
position: 143
reference: P06621-1

22571007532478766tt158
type: Position
type: ExactPosition
position: 158
reference: P06621-1

22571007532478766tt164
type: Position
type: ExactPosition
position: 164
reference: P06621-1

22571007532478766tt173
type: Position
type: ExactPosition
position: 173
reference: P06621-1

22571007532478766tt177
type: Position
type: ExactPosition
position: 177
reference: P06621-1

22571007532478766tt178
type: Position
type: ExactPosition
position: 178
reference: P06621-1

22571007532478766tt182
type: Position
type: ExactPosition
position: 182
reference: P06621-1

22571007532478766tt183
type: Position
type: ExactPosition
position: 183
reference: P06621-1

22571007532478766tt192
type: Position
type: ExactPosition
position: 192
reference: P06621-1

22571007532478766tt194
type: Position
type: ExactPosition
position: 194
reference: P06621-1

22571007532478766tt198
type: Position
type: ExactPosition
position: 198
reference: P06621-1

22571007532478766tt208
type: Position
type: ExactPosition
position: 208
reference: P06621-1

22571007532478766tt213
type: Position
type: ExactPosition
position: 213
reference: P06621-1

22571007532478766tt215
type: Position
type: ExactPosition
position: 215
reference: P06621-1

22571007532478766tt224
type: Position
type: ExactPosition
position: 224
reference: P06621-1

22571007532478766tt230
type: Position
type: ExactPosition
position: 230
reference: P06621-1

22571007532478766tt232
type: Position
type: ExactPosition
position: 232
reference: P06621-1

22571007532478766tt234
type: Position
type: ExactPosition
position: 234
reference: P06621-1

22571007532478766tt236
type: Position
type: ExactPosition
position: 236
reference: P06621-1

22571007532478766tt240
type: Position
type: ExactPosition
position: 240
reference: P06621-1

22571007532478766tt251
type: Position
type: ExactPosition
position: 251
reference: P06621-1

22571007532478766tt252
type: Position
type: ExactPosition
position: 252
reference: P06621-1

22571007532478766tt254
type: Position
type: ExactPosition
position: 254
reference: P06621-1

22571007532478766tt257
type: Position
type: ExactPosition
position: 257
reference: P06621-1

22571007532478766tt260
type: Position
type: ExactPosition
position: 260
reference: P06621-1

22571007532478766tt261
type: Position
type: ExactPosition
position: 261
reference: P06621-1

22571007532478766tt270
type: Position
type: ExactPosition
position: 270
reference: P06621-1

22571007532478766tt279
type: Position
type: ExactPosition
position: 279
reference: P06621-1

22571007532478766tt291
type: Position
type: ExactPosition
position: 291
reference: P06621-1

22571007532478766tt292
type: Position
type: ExactPosition
position: 292
reference: P06621-1

22571007532478766tt306
type: Position
type: ExactPosition
position: 306
reference: P06621-1

22571007532478766tt315
type: Position
type: ExactPosition
position: 315
reference: P06621-1

22571007532478766tt322
type: Position
type: ExactPosition
position: 322
reference: P06621-1

22571007532478766tt330
type: Position
type: ExactPosition
position: 330
reference: P06621-1

22571007532478766tt346
type: Position
type: ExactPosition
position: 346
reference: P06621-1

22571007532478766tt352
type: Position
type: ExactPosition
position: 352
reference: P06621-1

22571007532478766tt355
type: Position
type: ExactPosition
position: 355
reference: P06621-1

22571007532478766tt356
type: Position
type: ExactPosition
position: 356
reference: P06621-1

22571007532478766tt358
type: Position
type: ExactPosition
position: 358
reference: P06621-1

22571007532478766tt363
type: Position
type: ExactPosition
position: 363
reference: P06621-1

22571007532478766tt366
type: Position
type: ExactPosition
position: 366
reference: P06621-1

22571007532478766tt367
type: Position
type: ExactPosition
position: 367
reference: P06621-1

22571007532478766tt369
type: Position
type: ExactPosition
position: 369
reference: P06621-1

22571007532478766tt380
type: Position
type: ExactPosition
position: 380
reference: P06621-1

22571007532478766tt382
type: Position
type: ExactPosition
position: 382
reference: P06621-1

22571007532478766tt386
type: Position
type: ExactPosition
position: 386
reference: P06621-1

22571007532478766tt388
type: Position
type: ExactPosition
position: 388
reference: P06621-1

22571007532478766tt391
type: Position
type: ExactPosition
position: 391
reference: P06621-1

22571007532478766tt393
type: Position
type: ExactPosition
position: 393
reference: P06621-1

22571007532478766tt394
type: Position
type: ExactPosition
position: 394
reference: P06621-1

22571007532478766tt396
type: Position
type: ExactPosition
position: 396
reference: P06621-1

22571007532478766tt397
type: Position
type: ExactPosition
position: 397
reference: P06621-1

22571007532478766tt412
type: Position
type: ExactPosition
position: 412
reference: P06621-1

2
subclass of: CHEBI_29105

1
subclass of: CHEBI_29105

P06621-1
type: Simple Sequence
modified: 1988-01-01
version: 1
precursor: true
mass: 43932
value: MRPSIHRTAIAAVLATAFVAGTALAQKRDNVLFQAATDEQPAVIKTLEKLVNIETGTGDAEGIAAAGNFLEAELKNLGFTVTRSKSAGLVVGDNIVGKIKGRGGKNLLLMSHMDTVYLKGILAKAPFRVEGDKAYGPGIADDKGGNAVILHTLKLLKEYGVRDYGTITVLFNTDEEKGSFGSRDLIQEEAKLADYVLSFEPTSAGDEKLSLGTSGIAYVQVNITGKASHAGAAPELGVNALVEASDLVLRTMNIDDKAKNLRFNWTIAKAGNVSNIIPASATLNADVRYARNEDFDAAMKTLEERAQQKKLPEADVKVIVTRGRPAFNAGEGGKKLVDKAVAYYKEAGGTLGVEERTGGGTDAAYAALSGKPVIESLGLPGFGYHSDKAEYVDISAIPRRLYMAARLIMDLGAGK

http://purl.uniprot.org/uniprot/P06621#attribution-609619A7DF3E53CE5E48EDEF8B8F9DD1
manual: true
evidence: ECO_0000250

http://purl.uniprot.org/uniprot/P06621#attribution-308AEA4B1190EFCD85C1415B5DF7D8E4
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/9083113

http://purl.uniprot.org/uniprot/P06621#attribution-080ED3982FEC43F7C43A0689717F3D20
manual: true
evidence: ECO_0000305

P96977
type: Protein
reviewed: true
created: 1998-07-15
modified: 2023-05-03
version: 57
mnemonic: CHRR_PSEUG
old mnemonic: CHRR_PSESP
citation: SIP654B9FD9FD2CF1FF
recommended name: SIP4EF0FE8EF6F42DEE
enzyme: http://purl.uniprot.org/enzyme/1.-.-.-
organism: http://purl.uniprot.org/taxonomy/79677
annotation: SIP59E46FDF5A5A6961
annotation: SIP80640135AE99DEDC
annotation: PRO_0000205509
existence: Inferred from Homology Existence
classified with: http://purl.uniprot.org/keywords/288
classified with: http://purl.uniprot.org/keywords/560
classified with: GO_0016491
sequence: P96977-1

SIP654B9FD9FD2CF1FF
type: Submission Citation
author: Suzuki T.
date: 1996-02
submitted to: EMBL/GenBank/DDBJ

P96977-citation-SIP654B9FD9FD2CF1FF
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

cd02146
type: Resource
database: CDD
comment: NfsA-like
signature sequence match: CDD cd02146 match 1

http://purl.uniprot.org/gene3d/3.40.109.10
type: Resource
database: Gene3D
comment: NADH Oxidase
signature sequence match: Gene3D 3.40.109.10 match 1

IPR016446
type: Resource
database: InterPro
comment: Flavin_OxRdtase_Frp

IPR029479
type: Resource
database: InterPro
comment: Nitroreductase

IPR000415
type: Resource
database: InterPro
comment: Nitroreductase-like

PTHR43425:SF3
type: Resource
database: PANTHER
comment: FMN REDUCTASE (NADPH)
signature sequence match: PANTHER PTHR43425:SF3 match 1

PTHR43425
type: Resource
database: PANTHER
comment: OXYGEN-INSENSITIVE NADPH NITROREDUCTASE
signature sequence match: PANTHER PTHR43425 match 1

PF00881
type: Resource
database: Pfam
comment: Nitroreductase
signature sequence match: Pfam PF00881 match 1

PIRSF005426
type: Resource
database: PIRSF
comment: Frp
signature sequence match: PIRSF PIRSF005426 match 1

SSF55469
type: Resource
database: SUPFAM
comment: FMN-dependent nitroreductase-like
signature sequence match: SUPFAM SSF55469 match 1

SIP4EF0FE8EF6F42DEE
type: Structured Name
full name: CR(VI) reductase
ec name: 1.-.-.-

chrR
type: Gene

SIP80640135AE99DEDC
type: Similarity Annotation
comment: Belongs to the flavin oxidoreductase frp family.

PRO_0000205509
type: Chain Annotation
comment: CR(VI) reductase
mass: 27540
range: 22580903187789614tt1tt243

22580903187789614tt1
type: Position
type: ExactPosition
position: 1
reference: P96977-1

22580903187789614tt243
type: Position
type: ExactPosition
position: 243
reference: P96977-1

P96977-1
type: Simple Sequence
modified: 1997-05-01
version: 1
mass: 27540
value: MVKELLRNHSSVRIYDGNPISKEIIEELIATAQMAATSHFVQAYSVIWVTDEEKKEKLGMLSGNPRQYETSGGAFVFCVDFKRLQSAGKLEGVDIVADSAENVLVGVADVSLFAQNFVVAAESMGYGICYIGGVRNKPEEISELFNLPEYVFPLFGLTIGVPARRNEVKPRLPVAAVLHENEYNTEKYEELLPAYNDTMEAYYNNRSSNRKIDNWTKQMADFLIEQRRPHIKDFLAKKGFNWK

http://purl.uniprot.org/uniprot/P96977#attribution-609619A7DF3E53CE5E48EDEF8B8F9DD1
manual: true
evidence: ECO_0000250

http://purl.uniprot.org/uniprot/P96977#attribution-080ED3982FEC43F7C43A0689717F3D20
manual: true
evidence: ECO_0000305

P17262
type: Protein
reviewed: true
created: 1990-08-01
modified: 2023-09-13
version: 95
mnemonic: DMPB_PSEUF
old mnemonic: DMPB_PSEPU
old mnemonic: DMPB_PSESP
citation: http://purl.uniprot.org/citations/2620833
citation: http://purl.uniprot.org/citations/2194577
recommended name: SIPAE6BB3ABA6E5EDDD
alternative name: SIP1AA450B16B071666
alternative name: SIP9AD16B8F141FBA49
enzyme: http://purl.uniprot.org/enzyme/1.13.11.2
organism: http://purl.uniprot.org/taxonomy/79676
encoded in: pVI150
annotation: SIPBBB7469141831193
annotation: SIPAE0E2758BE6B33B4
annotation: SIPFE522E06418852AA
annotation: SIP45F47F4BD50DBE23
annotation: SIP220B6481128035CF
annotation: PRO_0000085029
annotation: SIP4C37FD74A90775BC
annotation: SIPBAC09F4D08B24292
annotation: SIP105913888C659B9D
annotation: SIP1103B924FC716139
annotation: SIP471A94397AB893F6
existence: Inferred from Homology Existence
classified with: http://purl.uniprot.org/keywords/58
classified with: http://purl.uniprot.org/keywords/223
classified with: http://purl.uniprot.org/keywords/408
classified with: http://purl.uniprot.org/keywords/479
classified with: http://purl.uniprot.org/keywords/614
classified with: http://purl.uniprot.org/keywords/677
classified with: GO_0018577
classified with: GO_0008198
classified with: GO_0043640
sequence: P17262-1

P17262-citation-2620833
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

P17262-citation-2194577
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 287-307

cd07243
type: Resource
database: CDD
comment: 2_3_CTD_C
signature sequence match: CDD cd07243 match 1

cd07265
type: Resource
database: CDD
comment: 2_3_CTD_N
signature sequence match: CDD cd07265 match 1

IPR017624
type: Resource
database: InterPro
comment: Catechol_2-3_dOase

TIGR03211
type: Resource
database: NCBIfam
comment: catechol_2_3
signature sequence match: NCBIfam TIGR03211 match 1

SIPAE6BB3ABA6E5EDDD
type: Structured Name
full name: Metapyrocatechase
short name: MPC
ec name: 1.13.11.2

SIP1AA450B16B071666
type: Structured Name
full name: CatO2ase

SIP9AD16B8F141FBA49
type: Structured Name
full name: Catechol 2,3-dioxygenase
short name: C23O

pVI150
type: Plasmid

dmpB
type: Gene

SIP45F47F4BD50DBE23
type: Subunit Annotation
comment: Homotetramer.

SIP220B6481128035CF
type: Similarity Annotation
comment: Belongs to the extradiol ring-cleavage dioxygenase family.

PRO_0000085029
type: Chain Annotation
comment: Metapyrocatechase
mass: 35255
range: 22572111272227374tt1tt307

22572111272227374tt1
type: Position
type: ExactPosition
position: 1
reference: P17262-1

22572111272227374tt307
type: Position
type: ExactPosition
position: 307
reference: P17262-1

SIP4C37FD74A90775BC
type: Domain Extent Annotation
comment: VOC 1
range: 22572111272227374tt7tt122

22572111272227374tt7
type: Position
type: ExactPosition
position: 7
reference: P17262-1

22572111272227374tt122
type: Position
type: ExactPosition
position: 122
reference: P17262-1

SIPBAC09F4D08B24292
type: Domain Extent Annotation
comment: VOC 2
range: 22572111272227374tt150tt269

22572111272227374tt150
type: Position
type: ExactPosition
position: 150
reference: P17262-1

22572111272227374tt269
type: Position
type: ExactPosition
position: 269
reference: P17262-1

22572111272227374tt153
type: Position
type: ExactPosition
position: 153
reference: P17262-1

22572111272227374tt214
type: Position
type: ExactPosition
position: 214
reference: P17262-1

22572111272227374tt265
type: Position
type: ExactPosition
position: 265
reference: P17262-1

P17262-1
type: Simple Sequence
modified: 1990-08-01
version: 1
mass: 35255
value: MKKGVMRPGHVQLRVLNLESALAHYRDLLGLIEMDRDEQGRVYLKAWTEVDKFSVVLREADQPGMDFMGFKVIDEDCLNRLTQDLLNYGCLIETIPAGELKGCGRRVRFQTPSGHFFELYADKEYTGKWGLEEINPEAWPRNLKGMRAVRFDHCLLYGDELQATYALFTEVLGFYLAEQVIDDDGTRVAQFLSLSTKAHDVAFIHCPEKGKFHHVSFFLETWEDVLRAADLISMTDTSIDIGPTRHGLTHGKTIYFFDPSGNRNEVFCGGDYNYQDHKPVTWLAKDLGKAIFYHDRVLNERFLTVLT

http://purl.uniprot.org/uniprot/P17262#attribution-609619A7DF3E53CE5E48EDEF8B8F9DD1
manual: true
evidence: ECO_0000250

http://purl.uniprot.org/uniprot/P17262#attribution-05C16B64E576893F0FB5E6F0F0FB0983
manual: true
evidence: ECO_0000255
source: PRU01163

http://purl.uniprot.org/uniprot/P17262#attribution-080ED3982FEC43F7C43A0689717F3D20
manual: true
evidence: ECO_0000305

P19059
type: Protein
reviewed: true
created: 1990-11-01
modified: 2023-09-13
version: 89
mnemonic: DMPC_PSEUF
old mnemonic: DMPC_PSEPU
old mnemonic: DMPC_PSESP
citation: http://purl.uniprot.org/citations/2194577
recommended name: SIPCDB369B7BAE04F28
enzyme: http://purl.uniprot.org/enzyme/1.2.1.85
organism: http://purl.uniprot.org/taxonomy/79676
encoded in: pVI150
annotation: SIP3636CD9FFF259954
annotation: SIP0737CAEFC439FD6B
annotation: SIP06928ECB92FC99CE
annotation: SIP8C59C79F418631D7
annotation: PRO_0000056588
annotation: SIP209CC18EDA800FD1
annotation: SIP95C25E518CAC995F
existence: Inferred from Homology Existence
classified with: http://purl.uniprot.org/keywords/58
classified with: http://purl.uniprot.org/keywords/520
classified with: http://purl.uniprot.org/keywords/560
classified with: http://purl.uniprot.org/keywords/614
classified with: GO_0016620
classified with: GO_0043640
sequence: P19059-1

P19059-citation-2194577
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

cd07093
type: Resource
database: CDD
comment: ALDH_F8_HMSADH
signature sequence match: CDD cd07093 match 1

IPR016161
type: Resource
database: InterPro
comment: Ald_DH/histidinol_DH

IPR016163
type: Resource
database: InterPro
comment: Ald_DH_C

IPR016160
type: Resource
database: InterPro
comment: Ald_DH_CS_CYS

IPR029510
type: Resource
database: InterPro
comment: Ald_DH_CS_GLU

IPR016162
type: Resource
database: InterPro
comment: Ald_DH_N

IPR015590
type: Resource
database: InterPro
comment: Aldehyde_DH_dom

IPR017628
type: Resource
database: InterPro
comment: OHmuconic_semiald_DH

TIGR03216
type: Resource
database: NCBIfam
comment: OH_muco_semi_DH
signature sequence match: NCBIfam TIGR03216 match 1

PTHR43720
type: Resource
database: PANTHER
comment: 2-AMINOMUCONIC SEMIALDEHYDE DEHYDROGENASE
signature sequence match: PANTHER PTHR43720 match 1

PTHR43720:SF2
type: Resource
database: PANTHER
comment: 2-AMINOMUCONIC SEMIALDEHYDE DEHYDROGENASE
signature sequence match: PANTHER PTHR43720:SF2 match 1

PF00171
type: Resource
database: Pfam
comment: Aldedh
signature sequence match: Pfam PF00171 match 1

SSF53720
type: Resource
database: SUPFAM
comment: ALDH-like
signature sequence match: SUPFAM SSF53720 match 1

PS00070
type: Resource
database: PROSITE
comment: ALDEHYDE_DEHYDR_CYS
signature sequence match: PROSITE PS00070 match 1

PS00687
type: Resource
database: PROSITE
comment: ALDEHYDE_DEHYDR_GLU
signature sequence match: PROSITE PS00687 match 1

SIPCDB369B7BAE04F28
type: Structured Name
full name: 2-hydroxymuconic semialdehyde dehydrogenase
short name: HMSD
ec name: 1.2.1.85

pVI150
type: Plasmid

dmpC
type: Gene

SIP3636CD9FFF259954
type: Function Annotation
comment: 2-hydroxymuconic acid semialdehyde can be converted to 2-hydroxypent-2,4-dienoate either directly by the action of 2-hydroxymuconic semialdehyde hydrolase (HMSH) or by the action of three sequential enzymes, the first of which is HMSD.

SIP8C59C79F418631D7
type: Similarity Annotation
comment: Belongs to the aldehyde dehydrogenase family.

PRO_0000056588
type: Chain Annotation
comment: 2-hydroxymuconic semialdehyde dehydrogenase
mass: 51683
range: 22572119828543790tt1tt486

22572119828543790tt1
type: Position
type: ExactPosition
position: 1
reference: P19059-1

22572119828543790tt486
type: Position
type: ExactPosition
position: 486
reference: P19059-1

22572119828543790tt254
type: Position
type: ExactPosition
position: 254
reference: P19059-1

22572119828543790tt288
type: Position
type: ExactPosition
position: 288
reference: P19059-1

P19059-1
type: Simple Sequence
modified: 1990-11-01
version: 1
mass: 51683
value: MKEIKHFINGAFVGSASGRTFEDVNPANGQVIARVHEAGRAEVDAAVQAARAALKGPWGKMSVSERAEILHRVADGITARFDEFLEAECLDTGKPKSLASHIDIPRGAANFKVFADLLKNVATEAFEMATPDGSGAINYAVRRPKGVIGVISPWNLPLLLMTWKVGPALACGNTVVVKPSEETPLTTALLGEVMQAAGVPAGVYNVVHGFGPDSAGAFLTEHPDVNAITFTGETRTGEAIMRAAAKGVRPVSFELGGKNAGIVFADCDLDKAIEGSMRSVFANGGQVCLGTERLYVERPIFDEFVARLKAGAESLVIGTPDDPQANFGPLISLQHREKVLSYYQKAVDEGATVVTGGGVPEMPAELAGGAWVQPTIWTGLADGAAVVTEEIFGPCCHIRPFDREEEAVELANSLPYGLAATIWTENTSRAHRVAGQLEAGIVWVNSWFLRDLRTAFGGSKQSGIGREGGVHSLEFYTELKNICVKL

http://purl.uniprot.org/uniprot/P19059#attribution-609619A7DF3E53CE5E48EDEF8B8F9DD1
manual: true
evidence: ECO_0000250

http://purl.uniprot.org/uniprot/P19059#attribution-080ED3982FEC43F7C43A0689717F3D20
manual: true
evidence: ECO_0000305

P19076
type: Protein
reviewed: true
created: 1990-11-01
modified: 2023-05-03
version: 81
mnemonic: DMPD_PSEUF
old mnemonic: DMPD_PSEPU
old mnemonic: DMPD_PSESP
citation: http://purl.uniprot.org/citations/2194577
recommended name: SIP46B7E04B9132AA15
alternative name: SIPD35032829507AD3E
enzyme: http://purl.uniprot.org/enzyme/3.7.1.9
organism: http://purl.uniprot.org/taxonomy/79676
encoded in: pVI150
annotation: SIPE812E4CF3AB94FC0
annotation: SIP8E879FF7B2A58D73
annotation: SIP152C40ED42944A7E
annotation: SIPF3B95C444360A96B
annotation: PRO_0000207055
annotation: SIP882D1CD1A3C5B81E
annotation: SIPBA130E1C1B88D7A5
annotation: SIPA45BF72C4857060F
annotation: SIP49CE84C1E46726B2
existence: Inferred from Homology Existence
classified with: http://purl.uniprot.org/keywords/58
classified with: http://purl.uniprot.org/keywords/614
classified with: http://purl.uniprot.org/keywords/719
classified with: GO_0018775
classified with: GO_0052689
classified with: GO_0043640
sequence: P19076-1

P19076-citation-2194577
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

psesp-dmpd
type: Resource
database: ESTHER
comment: Carbon-carbon_bond_hydrolase

PTHR43798:SF31
type: Resource
database: PANTHER
comment: AB HYDROLASE SUPERFAMILY PROTEIN YCLE
signature sequence match: PANTHER PTHR43798:SF31 match 1

PTHR43798
type: Resource
database: PANTHER
comment: MONOACYLGLYCEROL LIPASE
signature sequence match: PANTHER PTHR43798 match 1

SIP46B7E04B9132AA15
type: Structured Name
full name: 2-hydroxymuconate semialdehyde hydrolase
short name: HMSH
ec name: 3.7.1.9

SIPD35032829507AD3E
type: Structured Name
full name: 2-hydroxymuconic semialdehyde hydrolase

pVI150
type: Plasmid

dmpD
type: Gene

SIPE812E4CF3AB94FC0
type: Function Annotation
comment: Catalyzes the conversion of 2-hydroxymuconate semialdehyde to 2-hydroxypent-2,4-dienoate.

SIPF3B95C444360A96B
type: Similarity Annotation
comment: Belongs to the DmpD/TodF/XylF esterase family.

PRO_0000207055
type: Chain Annotation
comment: 2-hydroxymuconate semialdehyde hydrolase
mass: 30965
range: 22572119828674094tt1tt283

22572119828674094tt1
type: Position
type: ExactPosition
position: 1
reference: P19076-1

22572119828674094tt283
type: Position
type: ExactPosition
position: 283
reference: P19076-1

SIP882D1CD1A3C5B81E
type: Domain Extent Annotation
comment: AB hydrolase-1
range: 22572119828674094tt32tt262

22572119828674094tt32
type: Position
type: ExactPosition
position: 32
reference: P19076-1

22572119828674094tt262
type: Position
type: ExactPosition
position: 262
reference: P19076-1

22572119828674094tt107
type: Position
type: ExactPosition
position: 107
reference: P19076-1

22572119828674094tt228
type: Position
type: ExactPosition
position: 228
reference: P19076-1

22572119828674094tt256
type: Position
type: ExactPosition
position: 256
reference: P19076-1

P19076-1
type: Simple Sequence
modified: 1990-11-01
version: 1
mass: 30965
value: MNAPQNSPEIGREIIAAGIRTNLHDSGAGFPLMMIHGSGPGVTAWANWRLVMPELAKSRRVIAPDMLGFGYSERPADAQYNRDVWVDHAVGVLDALEIEQADLVGNSFGGGIALALAIRHPERVRRLVLMGSAGVSFPITEGLDAVWGYNPSFAEMRRLLDIFAFDRNLVNDELAELRYQASIRPGFHESFAAMFPAPRQRWVDGLASAEAAIRALPHETLVIHGREDQIIPLQTSLTLADWIARAQLHVFGQCGHWTQIEHAARFASLVGDFLAEADAAAIS

http://purl.uniprot.org/uniprot/P19076#attribution-609619A7DF3E53CE5E48EDEF8B8F9DD1
manual: true
evidence: ECO_0000250

http://purl.uniprot.org/uniprot/P19076#attribution-46BAF9912BAC37AEF4C6D095313B9117
manual: true
evidence: ECO_0000255

http://purl.uniprot.org/uniprot/P19076#attribution-080ED3982FEC43F7C43A0689717F3D20
manual: true
evidence: ECO_0000305

P49156
type: Protein
reviewed: true
created: 1996-02-01
modified: 2023-09-13
version: 70
mnemonic: DMPH_PSEUF
old mnemonic: DMPH_PSEPU
old mnemonic: DMPH_PSESP
citation: http://purl.uniprot.org/citations/1732207
recommended name: SIPCB758D6F9BB61D35
enzyme: http://purl.uniprot.org/enzyme/4.1.1.77
organism: http://purl.uniprot.org/taxonomy/79676
encoded in: pVI150
annotation: SIP37359C5B6B36F672
annotation: SIP10E1DB09EF6586D6
annotation: SIPB116A7EA5AF12A21
annotation: PRO_0000079939
existence: Inferred from Homology Existence
classified with: http://purl.uniprot.org/keywords/58
classified with: http://purl.uniprot.org/keywords/456
classified with: http://purl.uniprot.org/keywords/614
classified with: GO_0047437
classified with: GO_0043640
sequence: P49156-1

P49156-citation-1732207
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

http://purl.uniprot.org/gene3d/3.90.850.10
type: Resource
database: Gene3D
comment: Fumarylacetoacetase-like, C-terminal domain
signature sequence match: Gene3D 3.90.850.10 match 1

IPR017630
type: Resource
database: InterPro
comment: 4-oxalocrotonate_decarboxylase

IPR036663
type: Resource
database: InterPro
comment: Fumarylacetoacetase_C_sf

TIGR03218
type: Resource
database: NCBIfam
comment: catechol_dmpH
signature sequence match: NCBIfam TIGR03218 match 1

PTHR30143:SF0
type: Resource
database: PANTHER
comment: 2-KETO-4-PENTENOATE HYDRATASE
signature sequence match: PANTHER PTHR30143:SF0 match 1

PTHR30143
type: Resource
database: PANTHER
comment: ACID HYDRATASE
signature sequence match: PANTHER PTHR30143 match 1

SSF56529
type: Resource
database: SUPFAM
comment: FAH
signature sequence match: SUPFAM SSF56529 match 1

SIPCB758D6F9BB61D35
type: Structured Name
full name: 4-oxalocrotonate decarboxylase
short name: 4-OD
ec name: 4.1.1.77

pVI150
type: Plasmid

dmpH
type: Gene

SIPB116A7EA5AF12A21
type: Similarity Annotation
comment: Belongs to the hydratase/decarboxylase family.

PRO_0000079939
type: Chain Annotation
comment: 4-oxalocrotonate decarboxylase
mass: 28427
range: 22575418380203566tt1tt264

22575418380203566tt1
type: Position
type: ExactPosition
position: 1
reference: P49156-1

22575418380203566tt264
type: Position
type: ExactPosition
position: 264
reference: P49156-1

P49156-1
type: Simple Sequence
modified: 1996-02-01
version: 1
mass: 28427
value: MNRTLTRDQVLALAEHIENAELDVHDIPKVTNDYPDMTFADAYDVQWEIRRRKEARGNKVVGLKMGLTSWAKMAQMGVETPIYGFLVDYFSVPDGGVVDTSKLIHPKIEAEISFVTKAPLHGPGCHIGQVLAATDFVIPTVEVIDSRYENFKFDLISVVADNASSTRFITGGQMANVADLDLRTLGVVMEKNGEVVELGAGAAVLGHPASSVAMLANLLAERGEHIPAGSFIMTGGITAAVPVAPGDNITVRYQGLCSVSARFI

http://purl.uniprot.org/uniprot/P49156#attribution-080ED3982FEC43F7C43A0689717F3D20
manual: true
evidence: ECO_0000305

P19729
type: Protein
reviewed: true
created: 1991-02-01
modified: 2023-09-13
version: 68
mnemonic: DMPK_PSEUF
old mnemonic: DMPK_PSEPU
old mnemonic: DMPK_PSESP
citation: http://purl.uniprot.org/citations/2254258
citation: SIPAF0C52FCB7DC8F06
citation: http://purl.uniprot.org/citations/2254259
citation: http://purl.uniprot.org/citations/8995386
recommended name: SIPB779B97DBE2D1AD2
alternative name: SIP5C258063F6453F82
alternative name: SIP8C6A2712A0BB7C0F
organism: http://purl.uniprot.org/taxonomy/79676
encoded in: pVI150
annotation: SIP24993B045B8B4E7F
annotation: SIPEAB183E7B9F35E1C
annotation: SIPA926BF039FF2E567
annotation: SIPCBE1A11D36AC633D
annotation: SIPC3BE56A4EFAD2F03
annotation: SIPBFB9280C529364D6
annotation: PRO_0000079940
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/58
classified with: http://purl.uniprot.org/keywords/614
classified with: GO_0018662
classified with: GO_0019336
sequence: MTVTNTPTPTFDQLTRYIRVRSEPEAKFVEFDFAIGHPELFVELVLPQDAFVKFCQHNRVVAMDEAMAKAVDDDMVKWRFGDVGRRLPKDPG

P19729-citation-2254258
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
scope: FUNCTION
scope: PATHWAY
scope: DISRUPTION PHENOTYPE

CF600
type: Strain

SIPAF0C52FCB7DC8F06
type: Submission Citation
author: Takeo M.
author: Maeda Y.
author: Okada H.
author: Miyama K.
author: Mori K.
author: Ike M.
author: Fujita M.
date: 1994-03
submitted to: EMBL/GenBank/DDBJ

P19729-citation-SIPAF0C52FCB7DC8F06
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

BH
type: Strain

P19729-citation-2254259
type: Citation Statement
scope: FUNCTION

P19729-citation-8995386
type: Citation Statement
scope: FUNCTION
scope: SUBUNIT
scope: INTERACTION WITH DMPL AND DMPN
scope: MASS SPECTROMETRY

http://purl.uniprot.org/brenda/1.14.13.244
type: Resource
database: BRENDA
comment: 16277

IPR010353
type: Resource
database: InterPro
comment: DmpK

PF06099
type: Resource
database: Pfam
comment: Phenol_hyd_sub
signature sequence match: Pfam PF06099 match 1

PIRSF000039
type: Resource
database: PIRSF
comment: Phenol_monooxy_K
signature sequence match: PIRSF PIRSF000039 match 1

SIPB779B97DBE2D1AD2
type: Structured Name
full name: Phenol 2-monooxygenase, auxiliary component DmpK

SIP5C258063F6453F82
type: Structured Name
full name: Phenol 2-monooxygenase P0 component

SIP8C6A2712A0BB7C0F
type: Structured Name
full name: Phenol hydroxylase P0 protein

pVI150
type: Plasmid

dmpK
type: Gene
alternative label: pheA1

SIP24993B045B8B4E7F
type: Function Annotation
comment: DmpK is an auxiliary protein associated with the multicomponent phenol hydroxylase DmpLMNOP and it may be involved in the post-translational incorporation of iron into the oxygenase component of the phenol hydroxylase (PubMed:8995386). Required for growth on phenol but not for in vitro phenol hydroxylase activity (PubMed:2254258, PubMed:2254259).

SIPA926BF039FF2E567
type: Subunit Annotation
comment: Homotrimer or homotetramer (PubMed:8995386). Interacts with the phenol hydroxylase components DmpL (P1 component) and DmpN (P3 component) (PubMed:8995386).

SIPCBE1A11D36AC633D
type: Mass Spectrometry Annotation
measured value: 10451
method: ESI

SIPC3BE56A4EFAD2F03
type: Disruption Phenotype Annotation
comment: Cells lacking this gene cannot grow on phenol.

SIPBFB9280C529364D6
type: Initiator Methionine Annotation
comment: Removed
range: 22572119945787694tt1tt1

22572119945787694tt1
type: Position
type: ExactPosition
position: 1
reference: MTVTNTPTPTFDQLTRYIRVRSEPEAKFVEFDFAIGHPELFVELVLPQDAFVKFCQHNRVVAMDEAMAKAVDDDMVKWRFGDVGRRLPKDPG

PRO_0000079940
type: Chain Annotation
comment: Phenol 2-monooxygenase, auxiliary component DmpK
mass: 10455
range: 22572119945787694tt2tt92

22572119945787694tt2
type: Position
type: ExactPosition
position: 2
reference: MTVTNTPTPTFDQLTRYIRVRSEPEAKFVEFDFAIGHPELFVELVLPQDAFVKFCQHNRVVAMDEAMAKAVDDDMVKWRFGDVGRRLPKDPG

22572119945787694tt92
type: Position
type: ExactPosition
position: 92
reference: MTVTNTPTPTFDQLTRYIRVRSEPEAKFVEFDFAIGHPELFVELVLPQDAFVKFCQHNRVVAMDEAMAKAVDDDMVKWRFGDVGRRLPKDPG

MTVTNTPTPTFDQLTRYIRVRSEPEAKFVEFDFAIGHPELFVELVLPQDAFVKFCQHNRVVAMDEAMAKAVDDDMVKWRFGDVGRRLPKDPG
type: Simple Sequence
modified: 1991-02-01
version: 1
mass: 10586

http://purl.uniprot.org/uniprot/P19729#attribution-E7A1CB48C427DF76824484E9BB49D5AF
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/2254258

http://purl.uniprot.org/uniprot/P19729#attribution-E06BAF8C5982F03936E71B04F9F695C8
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/2254259

http://purl.uniprot.org/uniprot/P19729#attribution-07F2D3190ABD5B1838F105A1977F1CA6
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/8995386

http://purl.uniprot.org/uniprot/P19729#attribution-EAECA9725E4E460BC14B627E888B6588
manual: true
evidence: ECO_0000303
source: http://purl.uniprot.org/citations/2254258

http://purl.uniprot.org/uniprot/P19729#attribution-DA9616B946418E639F33F60BFD375F6C
manual: true
evidence: ECO_0000305

P19730
type: Protein
reviewed: true
created: 1991-02-01
modified: 2023-09-13
version: 87
mnemonic: DMPL_PSEUF
old mnemonic: DMPL_PSEPU
old mnemonic: DMPL_PSESP
citation: http://purl.uniprot.org/citations/2254258
citation: SIPAF0C52FCB7DC8F06
citation: http://purl.uniprot.org/citations/2254259
citation: http://purl.uniprot.org/citations/8995386
citation: http://purl.uniprot.org/citations/12186554
recommended name: SIPB44BFC2E312E0755
alternative name: SIP5F9FFF69C0742230
alternative name: SIPFC8AB3EBBDBB8743
enzyme: http://purl.uniprot.org/enzyme/1.14.13.244
organism: http://purl.uniprot.org/taxonomy/79676
encoded in: pVI150
annotation: SIP3BEE63CBD53D262A
annotation: SIP2F584E043A92B325
annotation: SIP4FF97CA23A9452E1
annotation: SIPCA3DD4A5EF970D63
annotation: SIP53B5B2E0DE50D2CA
annotation: SIP1A720585FC356281
annotation: SIP634A4E013FE02C79
annotation: PRO_0000079942
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/58
classified with: http://purl.uniprot.org/keywords/503
classified with: http://purl.uniprot.org/keywords/520
classified with: http://purl.uniprot.org/keywords/614
classified with: GO_0005727
classified with: GO_0018662
classified with: GO_0046191
sequence: P19730-1

P19730-citation-2254258
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
scope: FUNCTION
scope: PATHWAY
scope: DISRUPTION PHENOTYPE

CF600
type: Strain

P19730-citation-SIPAF0C52FCB7DC8F06
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

BH
type: Strain

P19730-citation-2254259
type: Citation Statement
scope: FUNCTION
scope: CATALYTIC ACTIVITY
scope: SUBUNIT

P19730-citation-8995386
type: Citation Statement
scope: INTERACTION WITH DMPK

P19730-citation-12186554
type: Citation Statement
scope: FUNCTION
scope: ACTIVITY REGULATION
scope: SUBUNIT

cd01058
type: Resource
database: CDD
comment: AAMH_B
signature sequence match: CDD cd01058 match 1

http://purl.uniprot.org/gene3d/1.10.620.20
type: Resource
database: Gene3D
comment: Ribonucleotide Reductase, subunit A
signature sequence match: Gene3D 1.10.620.20 match 1

IPR009078
type: Resource
database: InterPro
comment: Ferritin-like_SF

IPR012078
type: Resource
database: InterPro
comment: MP_mOase_hydro

IPR003430
type: Resource
database: InterPro
comment: Phenol_Hydrox

IPR012348
type: Resource
database: InterPro
comment: RNR-like

PF02332
type: Resource
database: Pfam
comment: Phenol_Hydrox
signature sequence match: Pfam PF02332 match 1

PIRSF000040
type: Resource
database: PIRSF
comment: MMOH_comp
signature sequence match: PIRSF PIRSF000040 match 1

SSF47240
type: Resource
database: SUPFAM
comment: Ferritin-like
signature sequence match: SUPFAM SSF47240 match 1

SIPB44BFC2E312E0755
type: Structured Name
full name: Phenol 2-monooxygenase, oxygenase component DmpL
ec name: 1.14.13.244

SIP5F9FFF69C0742230
type: Structured Name
full name: Phenol 2-monooxygenase P1 component

SIPFC8AB3EBBDBB8743
type: Structured Name
full name: Phenol hydroxylase P1 protein

pVI150
type: Plasmid

dmpL
type: Gene
alternative label: pheA2

SIP3BEE63CBD53D262A
type: Function Annotation
comment: Part of a multicomponent enzyme which catalyzes the degradation of phenol and some of its methylated derivatives (PubMed:2254259). DmpL, DmpN and DmpO form the oxygenase component of the complex (PubMed:12186554). Required for growth on phenol and for in vitro phenol hydroxylase activity (PubMed:2254258, PubMed:2254259).

SIP4FF97CA23A9452E1
type: Activity Regulation Annotation
comment: Requires DmpM for efficient turnover. The activity of DmpLNO oxygenase is inhibited by dithiothreitol (DTT) by a mechanism apparently involving H(2)O(2) generation.

SIP53B5B2E0DE50D2CA
type: Subunit Annotation
comment: The multicomponent enzyme phenol hydroxylase is formed by DmpL (P1 component), DmpM (P2 component), DmpN (P3 component), DmpO (P4 component) and DmpP (P5 component) (PubMed:2254259). The oxygenase component is a dimer composed of three subunits, DmpL, DmpN and DmpO (DmpLNO) (PubMed:12186554). DmpL interacts with the auxiliary protein DmpK (P0 component) (PubMed:8995386).

SIP1A720585FC356281
type: Disruption Phenotype Annotation
comment: Cells lacking this gene cannot grow on phenol.

SIP634A4E013FE02C79
type: Similarity Annotation
comment: Belongs to the TmoE/XamoE family.

PRO_0000079942
type: Chain Annotation
comment: Phenol 2-monooxygenase, oxygenase component DmpL
mass: 38208
range: 22572119945850926tt1tt331

22572119945850926tt1
type: Position
type: ExactPosition
position: 1
reference: P19730-1

22572119945850926tt331
type: Position
type: ExactPosition
position: 331
reference: P19730-1

P19730-1
type: Simple Sequence
modified: 1991-02-01
version: 1
mass: 38208
value: MSVEIKTNTVDPIRQTYGNLQRRFGDKPASRYQEASYDIEAVTNFHYRPLWDPQHELHDPTRTAIRMTDWHKVTDPRQFYYGAYVQTRARMQEATEHAYGFCEKRELLSRLPAELQAKLLRCLVPLRHAELGANMNNSSIAGDSIAATVTQMHIYQAMDRLGMGQYLSRIGLLLDGGTGEALDQAKAYWLDDPIWQGLRRYVEDSFVIRDWFELGLAQNLVLDGLLQPLMYQRFDQWLTENGGSDVAMLTEFMRDWYGESTRWVDAMFKTVLAENDANREQVQAWLEVWEPRAYEALLPLAEEATGIAALDEVRSAFATRLQKIGLKSREE

http://purl.uniprot.org/uniprot/P19730#attribution-A59DCF55C712C29915A710504C9BB97F
creator: UniProtKB
manual: true
evidence: ECO_0000314
source: http://purl.uniprot.org/citations/2254258

http://purl.uniprot.org/uniprot/P19730#attribution-5334FADD9DB3C2F83FCFA70B661C8579
creator: UniProtKB
manual: true
evidence: ECO_0000314
source: http://purl.uniprot.org/citations/225425

http://purl.uniprot.org/uniprot/P19730#attribution-003A13815F122FA327A78C944CE2B657
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/12186554

http://purl.uniprot.org/uniprot/P19730#attribution-E7A1CB48C427DF76824484E9BB49D5AF
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/2254258

http://purl.uniprot.org/uniprot/P19730#attribution-9FDC666876D0F03E2C6C87D589D6C5CB
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/2254259

http://purl.uniprot.org/uniprot/P19730#attribution-07F2D3190ABD5B1838F105A1977F1CA6
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/8995386

http://purl.uniprot.org/uniprot/P19730#attribution-EAECA9725E4E460BC14B627E888B6588
manual: true
evidence: ECO_0000303
source: http://purl.uniprot.org/citations/2254258

http://purl.uniprot.org/uniprot/P19730#attribution-DA9616B946418E639F33F60BFD375F6C
manual: true
evidence: ECO_0000305

P19731
type: Protein
reviewed: true
created: 1991-02-01
modified: 2023-09-13
version: 96
mnemonic: DMPM_PSEUF
old mnemonic: DMPM_PSEPU
old mnemonic: DMPM_PSESP
citation: http://purl.uniprot.org/citations/2254258
citation: SIPAF0C52FCB7DC8F06
citation: http://purl.uniprot.org/citations/2254259
citation: http://purl.uniprot.org/citations/10451366
citation: http://purl.uniprot.org/citations/12186554
citation: http://purl.uniprot.org/citations/9012665
recommended name: SIP4C6F8354AF4DB202
alternative name: SIPD6FEE65239B485F9
alternative name: SIP47A3ECCF22C927FE
enzyme: http://purl.uniprot.org/enzyme/1.14.13.244
organism: http://purl.uniprot.org/taxonomy/79676
encoded in: pVI150
annotation: SIPD850B5BAAFC3A9CA
annotation: SIP147D58A794748B54
annotation: SIPEE3DF021A988B232
annotation: SIP9482CC9F8F01D18A
annotation: SIP5338F299923C4E03
annotation: SIP4D67D18C5FA39DFA
annotation: SIP0C1E5BEFA12ADB0E
annotation: SIP7077523B758D4771
annotation: PRO_0000079943
annotation: SIPC00EC0FF5C5103B8
annotation: SIPDE5AA1AF972EA627
annotation: SIPEEBD73A2EB3602C8
annotation: SIP519ABBACB67D1070
annotation: SIPC1324C0EC5185481
annotation: SIP5C86DC4B4DCA2B61
annotation: SIP94E2E661C25F3E9A
annotation: SIP5C0D9C0F780D57DC
annotation: SIP99F6D38F1AEF0469
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/2
classified with: http://purl.uniprot.org/keywords/58
classified with: http://purl.uniprot.org/keywords/503
classified with: http://purl.uniprot.org/keywords/520
classified with: http://purl.uniprot.org/keywords/614
classified with: GO_0018662
classified with: GO_0019336
sequence: MSSLVYIAFQDNDNARYVVEAIIQDNPHAVVQHHPAMIRIEAEKRLEIRRETVEENLGRAWDVQEMLVDVITIGGNVDEDDDRFVLEWKN

P19731-citation-2254258
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
scope: FUNCTION
scope: PATHWAY
scope: DISRUPTION PHENOTYPE

CF600
type: Strain

P19731-citation-SIPAF0C52FCB7DC8F06
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

BH
type: Strain

P19731-citation-2254259
type: Citation Statement
scope: FUNCTION
scope: CATALYTIC ACTIVITY
scope: SUBUNIT

P19731-citation-10451366
type: Citation Statement
scope: FUNCTION
scope: SUBUNIT
scope: MASS SPECTROMETRY

P19731-citation-12186554
type: Citation Statement
scope: FUNCTION

P19731-citation-9012665
type: Citation Statement
scope: STRUCTURE BY NMR

PDB 1HQI tt1tt90
chain: A=1-90

http://purl.uniprot.org/gene3d/3.90.56.10
type: Resource
database: Gene3D
comment: Monooxygenase component MmoB/DmpM
signature sequence match: Gene3D 3.90.56.10 match 1

IPR003454
type: Resource
database: InterPro
comment: MOase_MmoB_DmpM

IPR036889
type: Resource
database: InterPro
comment: mOase_MmoB_DmpM_sf

PF02406
type: Resource
database: Pfam
comment: MmoB_DmpM
signature sequence match: Pfam PF02406 match 1

SSF56029
type: Resource
database: SUPFAM
comment: Monooxygenase (hydroxylase) regulatory protein
signature sequence match: SUPFAM SSF56029 match 1

SIP4C6F8354AF4DB202
type: Structured Name
full name: Phenol 2-monooxygenase, stimulatory component DmpM
ec name: 1.14.13.244

SIPD6FEE65239B485F9
type: Structured Name
full name: Phenol 2-monooxygenase P2 component

SIP47A3ECCF22C927FE
type: Structured Name
full name: Phenol hydroxylase P2 protein

pVI150
type: Plasmid

dmpM
type: Gene
alternative label: pheA3

SIPD850B5BAAFC3A9CA
type: Function Annotation
comment: Part of a multicomponent enzyme which catalyzes the degradation of phenol and some of its methylated derivatives (PubMed:2254259). DmpM is a regulatory subunit that stimulates the phenol hydroxylase activity of the complex (PubMed:10451366, PubMed:12186554). The steady-state rate of phenol hydroxylase turnover is dependent on the DmpM concentration, with a maximum observed rate at about 1.5 DmpM per oxygenase monomer. Higher concentrations of DmpM inhibit phenol hydroxylase activity (PubMed:12186554). May act by altering the redox potential of the oxygenase (PubMed:12186554). Required for growth on phenol and for in vitro phenol hydroxylase activity (PubMed:2254258, PubMed:2254259).

SIP9482CC9F8F01D18A
type: Subunit Annotation
comment: Active as a monomer (PubMed:10451366). Formation of dimers inactivates the protein (PubMed:10451366). The multicomponent enzyme phenol hydroxylase is formed by DmpL (P1 component), DmpM (P2 component), DmpN (P3 component), DmpO (P4 component) and DmpP (P5 component) (PubMed:2254259).

SIP5338F299923C4E03
type: Mass Spectrometry Annotation
measured value: 10361
method: ESI

SIP4D67D18C5FA39DFA
type: Disruption Phenotype Annotation
comment: Cells lacking this gene cannot grow on phenol.

SIP0C1E5BEFA12ADB0E
type: Similarity Annotation
comment: Belongs to the TmoD/XamoD family.

SIP7077523B758D4771
type: Initiator Methionine Annotation
comment: Removed
range: 22572119945851182tt1tt1

22572119945851182tt1
type: Position
type: ExactPosition
position: 1
reference: MSSLVYIAFQDNDNARYVVEAIIQDNPHAVVQHHPAMIRIEAEKRLEIRRETVEENLGRAWDVQEMLVDVITIGGNVDEDDDRFVLEWKN

PRO_0000079943
type: Chain Annotation
comment: Phenol 2-monooxygenase, stimulatory component DmpM
mass: 10360
range: 22572119945851182tt2tt90

22572119945851182tt2
type: Position
type: ExactPosition
position: 2
reference: MSSLVYIAFQDNDNARYVVEAIIQDNPHAVVQHHPAMIRIEAEKRLEIRRETVEENLGRAWDVQEMLVDVITIGGNVDEDDDRFVLEWKN

22572119945851182tt90
type: Position
type: ExactPosition
position: 90
reference: MSSLVYIAFQDNDNARYVVEAIIQDNPHAVVQHHPAMIRIEAEKRLEIRRETVEENLGRAWDVQEMLVDVITIGGNVDEDDDRFVLEWKN

22572119945851182tt8
type: Position
type: ExactPosition
position: 8
reference: MSSLVYIAFQDNDNARYVVEAIIQDNPHAVVQHHPAMIRIEAEKRLEIRRETVEENLGRAWDVQEMLVDVITIGGNVDEDDDRFVLEWKN

22572119945851182tt10
type: Position
type: ExactPosition
position: 10
reference: MSSLVYIAFQDNDNARYVVEAIIQDNPHAVVQHHPAMIRIEAEKRLEIRRETVEENLGRAWDVQEMLVDVITIGGNVDEDDDRFVLEWKN

22572119945851182tt14
type: Position
type: ExactPosition
position: 14
reference: MSSLVYIAFQDNDNARYVVEAIIQDNPHAVVQHHPAMIRIEAEKRLEIRRETVEENLGRAWDVQEMLVDVITIGGNVDEDDDRFVLEWKN

22572119945851182tt25
type: Position
type: ExactPosition
position: 25
reference: MSSLVYIAFQDNDNARYVVEAIIQDNPHAVVQHHPAMIRIEAEKRLEIRRETVEENLGRAWDVQEMLVDVITIGGNVDEDDDRFVLEWKN

22572119945851182tt26
type: Position
type: ExactPosition
position: 26
reference: MSSLVYIAFQDNDNARYVVEAIIQDNPHAVVQHHPAMIRIEAEKRLEIRRETVEENLGRAWDVQEMLVDVITIGGNVDEDDDRFVLEWKN

22572119945851182tt28
type: Position
type: ExactPosition
position: 28
reference: MSSLVYIAFQDNDNARYVVEAIIQDNPHAVVQHHPAMIRIEAEKRLEIRRETVEENLGRAWDVQEMLVDVITIGGNVDEDDDRFVLEWKN

22572119945851182tt43
type: Position
type: ExactPosition
position: 43
reference: MSSLVYIAFQDNDNARYVVEAIIQDNPHAVVQHHPAMIRIEAEKRLEIRRETVEENLGRAWDVQEMLVDVITIGGNVDEDDDRFVLEWKN

22572119945851182tt48
type: Position
type: ExactPosition
position: 48
reference: MSSLVYIAFQDNDNARYVVEAIIQDNPHAVVQHHPAMIRIEAEKRLEIRRETVEENLGRAWDVQEMLVDVITIGGNVDEDDDRFVLEWKN

22572119945851182tt50
type: Position
type: ExactPosition
position: 50
reference: MSSLVYIAFQDNDNARYVVEAIIQDNPHAVVQHHPAMIRIEAEKRLEIRRETVEENLGRAWDVQEMLVDVITIGGNVDEDDDRFVLEWKN

22572119945851182tt58
type: Position
type: ExactPosition
position: 58
reference: MSSLVYIAFQDNDNARYVVEAIIQDNPHAVVQHHPAMIRIEAEKRLEIRRETVEENLGRAWDVQEMLVDVITIGGNVDEDDDRFVLEWKN

22572119945851182tt60
type: Position
type: ExactPosition
position: 60
reference: MSSLVYIAFQDNDNARYVVEAIIQDNPHAVVQHHPAMIRIEAEKRLEIRRETVEENLGRAWDVQEMLVDVITIGGNVDEDDDRFVLEWKN

22572119945851182tt62
type: Position
type: ExactPosition
position: 62
reference: MSSLVYIAFQDNDNARYVVEAIIQDNPHAVVQHHPAMIRIEAEKRLEIRRETVEENLGRAWDVQEMLVDVITIGGNVDEDDDRFVLEWKN

22572119945851182tt63
type: Position
type: ExactPosition
position: 63
reference: MSSLVYIAFQDNDNARYVVEAIIQDNPHAVVQHHPAMIRIEAEKRLEIRRETVEENLGRAWDVQEMLVDVITIGGNVDEDDDRFVLEWKN

22572119945851182tt68
type: Position
type: ExactPosition
position: 68
reference: MSSLVYIAFQDNDNARYVVEAIIQDNPHAVVQHHPAMIRIEAEKRLEIRRETVEENLGRAWDVQEMLVDVITIGGNVDEDDDRFVLEWKN

22572119945851182tt69
type: Position
type: ExactPosition
position: 69
reference: MSSLVYIAFQDNDNARYVVEAIIQDNPHAVVQHHPAMIRIEAEKRLEIRRETVEENLGRAWDVQEMLVDVITIGGNVDEDDDRFVLEWKN

22572119945851182tt72
type: Position
type: ExactPosition
position: 72
reference: MSSLVYIAFQDNDNARYVVEAIIQDNPHAVVQHHPAMIRIEAEKRLEIRRETVEENLGRAWDVQEMLVDVITIGGNVDEDDDRFVLEWKN

22572119945851182tt84
type: Position
type: ExactPosition
position: 84
reference: MSSLVYIAFQDNDNARYVVEAIIQDNPHAVVQHHPAMIRIEAEKRLEIRRETVEENLGRAWDVQEMLVDVITIGGNVDEDDDRFVLEWKN

22572119945851182tt88
type: Position
type: ExactPosition
position: 88
reference: MSSLVYIAFQDNDNARYVVEAIIQDNPHAVVQHHPAMIRIEAEKRLEIRRETVEENLGRAWDVQEMLVDVITIGGNVDEDDDRFVLEWKN

MSSLVYIAFQDNDNARYVVEAIIQDNPHAVVQHHPAMIRIEAEKRLEIRRETVEENLGRAWDVQEMLVDVITIGGNVDEDDDRFVLEWKN
type: Simple Sequence
modified: 1991-02-01
version: 1
mass: 10491

http://purl.uniprot.org/uniprot/P19731#attribution-6A06DCE7A96DC7A0DC7960B041F107E1
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/10451366

http://purl.uniprot.org/uniprot/P19731#attribution-003A13815F122FA327A78C944CE2B657
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/12186554

http://purl.uniprot.org/uniprot/P19731#attribution-E7A1CB48C427DF76824484E9BB49D5AF
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/2254258

http://purl.uniprot.org/uniprot/P19731#attribution-9FDC666876D0F03E2C6C87D589D6C5CB
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/2254259

http://purl.uniprot.org/uniprot/P19731#attribution-EAECA9725E4E460BC14B627E888B6588
manual: true
evidence: ECO_0000303
source: http://purl.uniprot.org/citations/2254258

http://purl.uniprot.org/uniprot/P19731#attribution-DA9616B946418E639F33F60BFD375F6C
manual: true
evidence: ECO_0000305

P19732
type: Protein
reviewed: true
created: 1991-02-01
modified: 2023-09-13
version: 86
mnemonic: DMPN_PSEUF
old mnemonic: DMPN_PSEPU
old mnemonic: DMPN_PSESP
citation: http://purl.uniprot.org/citations/2254258
citation: SIPAF0C52FCB7DC8F06
citation: http://purl.uniprot.org/citations/2254259
citation: http://purl.uniprot.org/citations/8995386
citation: http://purl.uniprot.org/citations/12186554
recommended name: SIP97047AAE3676F1F4
alternative name: SIP6DD3D4EB227E1EC8
alternative name: SIP944C84C93444EFC0
enzyme: http://purl.uniprot.org/enzyme/1.14.13.244
organism: http://purl.uniprot.org/taxonomy/79676
encoded in: pVI150
annotation: SIPBA22657CAF24374D
annotation: SIP32DA4BD27BD7A062
annotation: SIPA3CD4938E91192CD
annotation: SIP7CC76701A9A8E04A
annotation: SIP1EB4D303085D4725
annotation: SIPB8D292C011AC2068
annotation: SIPE59C058C47AD1AA3
annotation: SIP495A09443387DD7C
annotation: PRO_0000079945
annotation: SIPB7D2AE229C305446
annotation: SIPF6B8E67AC2591646
annotation: SIPCD619CEAF66B6F0F
annotation: SIP3846CCD636EC5B93
annotation: SIP7D0362AD7AD12D47
annotation: SIPB302E6C722F64B06
annotation: SIPAD0F31E2F3DE7356
annotation: SIP1DCE7223C8050FC3
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/58
classified with: http://purl.uniprot.org/keywords/408
classified with: http://purl.uniprot.org/keywords/479
classified with: http://purl.uniprot.org/keywords/503
classified with: http://purl.uniprot.org/keywords/520
classified with: http://purl.uniprot.org/keywords/614
classified with: GO_0046872
classified with: GO_0018662
classified with: GO_0019336
sequence: P19732-1

P19732-citation-2254258
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
scope: FUNCTION
scope: PATHWAY
scope: DISRUPTION PHENOTYPE

CF600
type: Strain

P19732-citation-SIPAF0C52FCB7DC8F06
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

BH
type: Strain

P19732-citation-2254259
type: Citation Statement
scope: FUNCTION
scope: CATALYTIC ACTIVITY
scope: SUBUNIT

P19732-citation-8995386
type: Citation Statement
scope: INTERACTION WITH DMPK

P19732-citation-12186554
type: Citation Statement
scope: FUNCTION
scope: COFACTOR
scope: ACTIVITY REGULATION
scope: SUBUNIT

cd01057
type: Resource
database: CDD
comment: AAMH_A
signature sequence match: CDD cd01057 match 1

IPR007029
type: Resource
database: InterPro
comment: YHS_dom

PF04945
type: Resource
database: Pfam
comment: YHS
signature sequence match: Pfam PF04945 match 1

SIP97047AAE3676F1F4
type: Structured Name
full name: Phenol 2-monooxygenase, oxygenase component DmpN
ec name: 1.14.13.244

SIP6DD3D4EB227E1EC8
type: Structured Name
full name: Phenol 2-monooxygenase P3 component

SIP944C84C93444EFC0
type: Structured Name
full name: Phenol hydroxylase P3 protein

pVI150
type: Plasmid

dmpN
type: Gene
alternative label: pheA4

SIPBA22657CAF24374D
type: Function Annotation
comment: Part of a multicomponent enzyme which catalyzes the degradation of phenol and some of its methylated derivatives (PubMed:2254259). DmpL, DmpN and DmpO form the oxygenase component of the complex (PubMed:12186554). Required for growth on phenol and for in vitro phenol hydroxylase activity (PubMed:2254258, PubMed:2254259).

SIPA3CD4938E91192CD
type: Cofactor Annotation
comment: Binds 2 Fe(2+) ions per subunit.
cofactor: CHEBI_29033

SIP7CC76701A9A8E04A
type: Activity Regulation Annotation
comment: Requires DmpM for efficient turnover. The activity of DmpLNO oxygenase is inhibited by dithiothreitol (DTT) by a mechanism apparently involving H(2)O(2) generation.

SIPB8D292C011AC2068
type: Subunit Annotation
comment: The multicomponent enzyme phenol hydroxylase is formed by DmpL (P1 component), DmpM (P2 component), DmpN (P3 component), DmpO (P4 component) and DmpP (P5 component) (PubMed:2254259). The oxygenase component is a dimer composed of three subunits, DmpL, DmpN and DmpO (DmpLNO) (PubMed:12186554). DmpN interacts with the auxiliary protein DmpK (P0 component) (PubMed:8995386).

SIPE59C058C47AD1AA3
type: Disruption Phenotype Annotation
comment: Cells lacking this gene cannot grow on phenol.

SIP495A09443387DD7C
type: Similarity Annotation
comment: Belongs to the TmoA/XamoA family.

PRO_0000079945
type: Chain Annotation
comment: Phenol 2-monooxygenase, oxygenase component DmpN
mass: 60523
range: 22572119945851438tt1tt517

22572119945851438tt1
type: Position
type: ExactPosition
position: 1
reference: P19732-1

22572119945851438tt517
type: Position
type: ExactPosition
position: 517
reference: P19732-1

22572119945851438tt109
type: Position
type: ExactPosition
position: 109
reference: P19732-1

22572119945851438tt139
type: Position
type: ExactPosition
position: 139
reference: P19732-1

22572119945851438tt142
type: Position
type: ExactPosition
position: 142
reference: P19732-1

22572119945851438tt200
type: Position
type: ExactPosition
position: 200
reference: P19732-1

22572119945851438tt234
type: Position
type: ExactPosition
position: 234
reference: P19732-1

22572119945851438tt237
type: Position
type: ExactPosition
position: 237
reference: P19732-1

1
subclass of: CHEBI_24875
comment: catalytic

2
subclass of: CHEBI_24875
comment: catalytic

P19732-1
type: Simple Sequence
modified: 1991-02-01
version: 1
mass: 60523
value: MATHNKKRLNLKDKYRYLTRDLAWETTYQKKEDVFPLEHFEGIKITDWDKWEDPFRLTMDTYWKYQAEKEKKLYAIFDAFAQNNGHQNISDARYVNALKLFLTAVSPLEYQAFQGFSRVGRQFSGAGARVACQMQAIDELRHVQTQVHAMSHYNKHFDGLHDFAHMYDRVWYLSVPKSYMDDARTAGPFEFLTAVSFSFEYVLTNLLFVPFMSGAAYNGDMATVTFGFSAQSDEARHMTLGLEVIKFMLEQHEDNVPIIQRWIDKWFWRGYRLLTLIGMMMDYMLPNKVMSWSEAWGVYFEQAGGALFKDLERYGIRPPKYVEQTTIGKEHITHQVWGALYQYSKATSFHTWIPGDEELNWLSEKYPDTFDKYYRPRFEFWREQQAKGERFYNDTLPHLCQVCQLPVIFTEPDDPTKLSLRSLVHEGERYQFCSDGCCDIFKNEPVKYIQAWLPVHQIYQGNCEGGDVETVVQKYYHIKSGVDNLEYLGSPEHQRWLALKGQTPPTAAPADKSLGAA

http://purl.uniprot.org/uniprot/P19732#attribution-0068A75D15C6A9186487E8C1A7C3968B
manual: true
evidence: ECO_0000250
source: Q00456

http://purl.uniprot.org/uniprot/P19732#attribution-003A13815F122FA327A78C944CE2B657
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/12186554

http://purl.uniprot.org/uniprot/P19732#attribution-E7A1CB48C427DF76824484E9BB49D5AF
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/2254258

http://purl.uniprot.org/uniprot/P19732#attribution-9FDC666876D0F03E2C6C87D589D6C5CB
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/2254259

http://purl.uniprot.org/uniprot/P19732#attribution-07F2D3190ABD5B1838F105A1977F1CA6
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/8995386

http://purl.uniprot.org/uniprot/P19732#attribution-EAECA9725E4E460BC14B627E888B6588
manual: true
evidence: ECO_0000303
source: http://purl.uniprot.org/citations/2254258

http://purl.uniprot.org/uniprot/P19732#attribution-DA9616B946418E639F33F60BFD375F6C
manual: true
evidence: ECO_0000305

P19733
type: Protein
reviewed: true
created: 1991-02-01
modified: 2023-09-13
version: 71
mnemonic: DMPO_PSEUF
old mnemonic: DMPO_PSEPU
old mnemonic: DMPO_PSESP
citation: http://purl.uniprot.org/citations/2254258
citation: SIPAF0C52FCB7DC8F06
citation: http://purl.uniprot.org/citations/2254259
citation: http://purl.uniprot.org/citations/12186554
recommended name: SIPF9452319E7F7716D
alternative name: SIP21BAF447F2BA0ADF
alternative name: SIP4485719B7FA18D9B
enzyme: http://purl.uniprot.org/enzyme/1.14.13.244
organism: http://purl.uniprot.org/taxonomy/79676
encoded in: pVI150
annotation: SIP18768C7B0D8F39F6
annotation: SIP2B0FF55D0F7D19E4
annotation: SIPC00D490DBEBB0866
annotation: SIP8C85EE3F920F6C7A
annotation: SIPD18290192105E99A
annotation: SIPCDFFEF55997E3677
annotation: PRO_0000079946
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/58
classified with: http://purl.uniprot.org/keywords/503
classified with: http://purl.uniprot.org/keywords/520
classified with: http://purl.uniprot.org/keywords/614
classified with: GO_0018662
classified with: GO_0019336
sequence: MTVNSIGEYTATPRDVQANFNGMQLLYLYWEEHLMYCSALAFLVAPGMPFAEFLEQVLKPAIHAHPDSAKIDFSQALWQLNDQPFTPDYAASLEANGIDHKSMLRLNTPGLNGIQGSCS

P19733-citation-2254258
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
scope: FUNCTION
scope: PATHWAY
scope: DISRUPTION PHENOTYPE

CF600
type: Strain

P19733-citation-SIPAF0C52FCB7DC8F06
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

BH
type: Strain

P19733-citation-2254259
type: Citation Statement
scope: FUNCTION
scope: CATALYTIC ACTIVITY
scope: SUBUNIT

P19733-citation-12186554
type: Citation Statement
scope: FUNCTION
scope: ACTIVITY REGULATION
scope: SUBUNIT

http://purl.uniprot.org/gene3d/3.10.20.560
type: Resource
database: Gene3D
comment: Phenol hydroxylase
signature sequence match: Gene3D 3.10.20.560 match 1

IPR006756
type: Resource
database: InterPro
comment: Phenol_hydroxylase

IPR043010
type: Resource
database: InterPro
comment: Phenol_hydroxylase_sf

PF04663
type: Resource
database: Pfam
comment: Phenol_monoox
signature sequence match: Pfam PF04663 match 1

SIPF9452319E7F7716D
type: Structured Name
full name: Phenol 2-monooxygenase, oxygenase component DmpO
ec name: 1.14.13.244

SIP21BAF447F2BA0ADF
type: Structured Name
full name: Phenol 2-monooxygenase P4 component

SIP4485719B7FA18D9B
type: Structured Name
full name: Phenol hydroxylase P4 protein

pVI150
type: Plasmid

dmpO
type: Gene
alternative label: pheA5

SIP18768C7B0D8F39F6
type: Function Annotation
comment: Part of a multicomponent enzyme which catalyzes the degradation of phenol and some of its methylated derivatives (PubMed:2254259). DmpL, DmpN and DmpO form the oxygenase component of the complex (PubMed:12186554). Required for growth on phenol and for in vitro phenol hydroxylase activity (PubMed:2254258, PubMed:2254259).

SIPC00D490DBEBB0866
type: Activity Regulation Annotation
comment: Requires DmpM for efficient turnover. The activity of DmpLNO oxygenase is inhibited by dithiothreitol (DTT) by a mechanism apparently involving H(2)O(2) generation.

SIPD18290192105E99A
type: Subunit Annotation
comment: The multicomponent enzyme phenol hydroxylase is formed by DmpL (P1 component), DmpM (P2 component), DmpN (P3 component), DmpO (P4 component) and DmpP (P5 component) (PubMed:2254259). The oxygenase component is a dimer composed of three subunits, DmpL, DmpN and DmpO (DmpLNO) (PubMed:12186554).

SIPCDFFEF55997E3677
type: Disruption Phenotype Annotation
comment: Cells lacking this gene cannot grow on phenol.

PRO_0000079946
type: Chain Annotation
comment: Phenol 2-monooxygenase, oxygenase component DmpO
mass: 13207
range: 22572119945851694tt1tt119

22572119945851694tt1
type: Position
type: ExactPosition
position: 1
reference: MTVNSIGEYTATPRDVQANFNGMQLLYLYWEEHLMYCSALAFLVAPGMPFAEFLEQVLKPAIHAHPDSAKIDFSQALWQLNDQPFTPDYAASLEANGIDHKSMLRLNTPGLNGIQGSCS

22572119945851694tt119
type: Position
type: ExactPosition
position: 119
reference: MTVNSIGEYTATPRDVQANFNGMQLLYLYWEEHLMYCSALAFLVAPGMPFAEFLEQVLKPAIHAHPDSAKIDFSQALWQLNDQPFTPDYAASLEANGIDHKSMLRLNTPGLNGIQGSCS

MTVNSIGEYTATPRDVQANFNGMQLLYLYWEEHLMYCSALAFLVAPGMPFAEFLEQVLKPAIHAHPDSAKIDFSQALWQLNDQPFTPDYAASLEANGIDHKSMLRLNTPGLNGIQGSCS
type: Simple Sequence
modified: 1991-02-01
version: 1
mass: 13207

http://purl.uniprot.org/uniprot/P19733#attribution-003A13815F122FA327A78C944CE2B657
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/12186554

http://purl.uniprot.org/uniprot/P19733#attribution-E7A1CB48C427DF76824484E9BB49D5AF
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/2254258

http://purl.uniprot.org/uniprot/P19733#attribution-9FDC666876D0F03E2C6C87D589D6C5CB
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/2254259

http://purl.uniprot.org/uniprot/P19733#attribution-EAECA9725E4E460BC14B627E888B6588
manual: true
evidence: ECO_0000303
source: http://purl.uniprot.org/citations/2254258

http://purl.uniprot.org/uniprot/P19733#attribution-DA9616B946418E639F33F60BFD375F6C
manual: true
evidence: ECO_0000305

P19734
type: Protein
reviewed: true
created: 1991-02-01
modified: 2023-09-13
version: 105
mnemonic: DMPP_PSEUF
old mnemonic: DMPP_PSEPU
old mnemonic: DMPP_PSESP
citation: http://purl.uniprot.org/citations/2254258
citation: http://purl.uniprot.org/citations/2254259
recommended name: SIP364BDC301D452CE8
alternative name: SIP26814276E209B620
alternative name: SIP21AA6CE7A53D8333
enzyme: http://purl.uniprot.org/enzyme/1.14.13.244
organism: http://purl.uniprot.org/taxonomy/79676
encoded in: pVI150
annotation: SIP5A896127FA23B2DF
annotation: SIP8F01FDA5D134722B
annotation: SIPFA735A8BAADC2FC3
annotation: SIP76988E2BB7B22757
annotation: SIP8C3C352BE2B439E9
annotation: SIP4B1D0E9B87FCF3BE
annotation: SIP58D44BD8AB15B8D2
annotation: SIP5FBA1675256D3232
annotation: PRO_0000189407
annotation: SIP93C822EA187DA34E
annotation: SIPBD51EAAF93889803
annotation: SIP09468F97B6CD8AC4
annotation: SIPAA2A6372BB426D8F
annotation: SIP01678D942B9552F1
annotation: SIPA1B68DE6E25E48D1
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/1
classified with: http://purl.uniprot.org/keywords/58
classified with: http://purl.uniprot.org/keywords/903
classified with: http://purl.uniprot.org/keywords/249
classified with: http://purl.uniprot.org/keywords/274
classified with: http://purl.uniprot.org/keywords/503
classified with: http://purl.uniprot.org/keywords/520
classified with: http://purl.uniprot.org/keywords/614
classified with: GO_0005727
classified with: GO_0051537
classified with: GO_0046872
classified with: GO_0018662
classified with: GO_0046191
sequence: P19734-1

P19734-citation-2254258
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
scope: FUNCTION
scope: PATHWAY
scope: DISRUPTION PHENOTYPE

CF600
type: Strain

P19734-citation-2254259
type: Citation Statement
scope: PROTEIN SEQUENCE OF 2-5
scope: FUNCTION
scope: CATALYTIC ACTIVITY
scope: COFACTOR
scope: SUBUNIT

cd00207
type: Resource
database: CDD
comment: fer2
signature sequence match: CDD cd00207 match 1

cd06211
type: Resource
database: CDD
comment: phenol_2-monooxygenase_like
signature sequence match: CDD cd06211 match 1

http://purl.uniprot.org/gene3d/3.40.50.80
type: Resource
database: Gene3D
comment: Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module
signature sequence match: Gene3D 3.40.50.80 match 1

http://purl.uniprot.org/gene3d/2.40.30.10
type: Resource
database: Gene3D
comment: Translation factors
signature sequence match: Gene3D 2.40.30.10 match 1

IPR036010
type: Resource
database: InterPro
comment: 2Fe-2S_ferredoxin-like_sf

IPR001041
type: Resource
database: InterPro
comment: 2Fe-2S_ferredoxin-type

IPR006058
type: Resource
database: InterPro
comment: 2Fe2S_fd_BS

IPR012675
type: Resource
database: InterPro
comment: Beta-grasp_dom_sf

IPR008333
type: Resource
database: InterPro
comment: Cbr1-like_FAD-bd_dom

IPR017927
type: Resource
database: InterPro
comment: FAD-bd_FR_type

IPR039261
type: Resource
database: InterPro
comment: FNR_nucleotide-bd

IPR001433
type: Resource
database: InterPro
comment: OxRdtase_FAD/NAD-bd

IPR017938
type: Resource
database: InterPro
comment: Riboflavin_synthase-like_b-brl

PTHR47354
type: Resource
database: PANTHER
comment: NADH OXIDOREDUCTASE HCR
signature sequence match: PANTHER PTHR47354 match 1

PTHR47354:SF5
type: Resource
database: PANTHER
comment: PROTEIN RFBI
signature sequence match: PANTHER PTHR47354:SF5 match 1

PF00970
type: Resource
database: Pfam
comment: FAD_binding_6
signature sequence match: Pfam PF00970 match 1

PF00111
type: Resource
database: Pfam
comment: Fer2
signature sequence match: Pfam PF00111 match 1

PF00175
type: Resource
database: Pfam
comment: NAD_binding_1
signature sequence match: Pfam PF00175 match 1

PR00410
type: Resource
database: PRINTS
comment: PHEHYDRXLASE

SSF54292
type: Resource
database: SUPFAM
comment: 2Fe-2S ferredoxin-like
signature sequence match: SUPFAM SSF54292 match 1

SSF52343
type: Resource
database: SUPFAM
comment: Ferredoxin reductase-like, C-terminal NADP-linked domain
signature sequence match: SUPFAM SSF52343 match 1

SSF63380
type: Resource
database: SUPFAM
comment: Riboflavin synthase domain-like
signature sequence match: SUPFAM SSF63380 match 1

PS00197
type: Resource
database: PROSITE
comment: 2FE2S_FER_1
signature sequence match: PROSITE PS00197 match 1

PS51085
type: Resource
database: PROSITE
comment: 2FE2S_FER_2
signature sequence match: PROSITE PS51085 match 1

PS51384
type: Resource
database: PROSITE
comment: FAD_FR
signature sequence match: PROSITE PS51384 match 1

SIP364BDC301D452CE8
type: Structured Name
full name: Phenol 2-monooxygenase, reductase component DmpP
ec name: 1.14.13.244

SIP26814276E209B620
type: Structured Name
full name: Phenol 2-monooxygenase P5 component

SIP21AA6CE7A53D8333
type: Structured Name
full name: Phenol hydroxylase P5 protein

pVI150
type: Plasmid

dmpP
type: Gene

SIP5A896127FA23B2DF
type: Function Annotation
comment: Part of a multicomponent enzyme which catalyzes the degradation of phenol and some of its methylated derivatives (PubMed:2254259). DmpP probably transfers electrons from NADH, via FAD and the iron-sulfur center, to the oxygenase component of the complex (PubMed:2254259). Required for growth on phenol and for in vitro phenol hydroxylase activity (PubMed:2254258, PubMed:2254259).

SIPFA735A8BAADC2FC3
type: Cofactor Annotation
comment: Binds 1 FAD per subunit.
cofactor: CHEBI_57692

SIP76988E2BB7B22757
type: Cofactor Annotation
comment: Binds 1 [2Fe-2S] cluster per subunit.
cofactor: CHEBI_190135

SIP4B1D0E9B87FCF3BE
type: Subunit Annotation
comment: The multicomponent enzyme phenol hydroxylase is formed by DmpL (P1 component), DmpM (P2 component), DmpN (P3 component), DmpO (P4 component) and DmpP (P5 component).

SIP58D44BD8AB15B8D2
type: Disruption Phenotype Annotation
comment: Cells lacking this gene cannot grow on phenol.

SIP5FBA1675256D3232
type: Initiator Methionine Annotation
comment: Removed
range: 22572119945851950tt1tt1

22572119945851950tt1
type: Position
type: ExactPosition
position: 1
reference: P19734-1

PRO_0000189407
type: Chain Annotation
comment: Phenol 2-monooxygenase, reductase component DmpP
mass: 38346
range: 22572119945851950tt2tt353

22572119945851950tt2
type: Position
type: ExactPosition
position: 2
reference: P19734-1

22572119945851950tt353
type: Position
type: ExactPosition
position: 353
reference: P19734-1

SIP93C822EA187DA34E
type: Domain Extent Annotation
comment: 2Fe-2S ferredoxin-type
range: 22572119945851950tt3tt93

22572119945851950tt3
type: Position
type: ExactPosition
position: 3
reference: P19734-1

22572119945851950tt93
type: Position
type: ExactPosition
position: 93
reference: P19734-1

SIPBD51EAAF93889803
type: Domain Extent Annotation
comment: FAD-binding FR-type
range: 22572119945851950tt102tt201

22572119945851950tt102
type: Position
type: ExactPosition
position: 102
reference: P19734-1

22572119945851950tt201
type: Position
type: ExactPosition
position: 201
reference: P19734-1

22572119945851950tt37
type: Position
type: ExactPosition
position: 37
reference: P19734-1

22572119945851950tt42
type: Position
type: ExactPosition
position: 42
reference: P19734-1

22572119945851950tt45
type: Position
type: ExactPosition
position: 45
reference: P19734-1

22572119945851950tt77
type: Position
type: ExactPosition
position: 77
reference: P19734-1

P19734-1
type: Simple Sequence
modified: 2007-01-23
version: 3
mass: 38478
value: MSYNVTIEPTGEVIEVEDGQTILQAALRQGVWLPFACGHGTCATCKVQVVEGEVDIGEASPFALMDIERDERKVLACCAIPLSDLVIEADVDADPDFLGHPVEDYRGVVSALVDLSPTIKGLHIKLDRPMPFQAGQYVNLALPGIDGTRAFSLANPPSRNDEVELHVRLVEGGAATGFIHKQLKVGDAVELSGPYGQFFVRDSQAGDLIFIAGGSGLSSPQSMILDLLERGDTRRITLFQGARNRAELYNCELFEELAARHPNFSYVPALNQANDDPEWQGFKGFVHDAAKAHFDGRFGGQKAYLCGPPPMIDAAITTLMQGRLFERDIFMERFYTAADGAGESSRSALFKRI

http://purl.uniprot.org/uniprot/P19734#attribution-A59DCF55C712C29915A710504C9BB97F
creator: UniProtKB
manual: true
evidence: ECO_0000314
source: http://purl.uniprot.org/citations/2254258

http://purl.uniprot.org/uniprot/P19734#attribution-8CD922394DDD74D49CEE1846EB030829
creator: UniProtKB
manual: true
evidence: ECO_0000314
source: http://purl.uniprot.org/citations/2254259

http://purl.uniprot.org/uniprot/P19734#attribution-4C287B5EE055C53751B747804F0BDCE6
manual: true
evidence: ECO_0000255
source: PRU00465

http://purl.uniprot.org/uniprot/P19734#attribution-C1CFAB25154B3C2AEBB8832C3B268CDC
manual: true
evidence: ECO_0000255
source: PRU00716

http://purl.uniprot.org/uniprot/P19734#attribution-E7A1CB48C427DF76824484E9BB49D5AF
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/2254258

http://purl.uniprot.org/uniprot/P19734#attribution-9FDC666876D0F03E2C6C87D589D6C5CB
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/2254259

http://purl.uniprot.org/uniprot/P19734#attribution-EAECA9725E4E460BC14B627E888B6588
manual: true
evidence: ECO_0000303
source: http://purl.uniprot.org/citations/2254258

http://purl.uniprot.org/uniprot/P19734#attribution-DA9616B946418E639F33F60BFD375F6C
manual: true
evidence: ECO_0000305

P33160
type: Protein
reviewed: true
created: 1993-10-01
modified: 2023-05-03
version: 122
mnemonic: FDH_PSESR
old mnemonic: FDH_PSESP
citation: http://purl.uniprot.org/citations/1954846
citation: http://purl.uniprot.org/citations/2357236
citation: http://purl.uniprot.org/citations/1597184
citation: http://purl.uniprot.org/citations/12144528
citation: http://purl.uniprot.org/citations/8484798
citation: http://purl.uniprot.org/citations/8114093
citation: SIPCACDB8762C58F134
citation: SIP513C82697291997D
conflicting sequence: SIPE71BA964542D970A
recommended name: SIPD7FC11992EABA9EE
alternative name: SIPCBFB018EED044498
enzyme: http://purl.uniprot.org/enzyme/1.17.1.9
organism: http://purl.uniprot.org/taxonomy/33067
annotation: SIPBEF5FB995478CD5A
annotation: SIP8687E3671742809E
annotation: SIPA340E0901765BE46
annotation: SIP4CB576D98ACDA2AC
annotation: SIP5A4BDB4542601631
annotation: SIP256FA1F2A44BC0B9
annotation: SIP213D047F3CFD4169
annotation: PRO_0000076027
annotation: SIPFC67483608F12510
annotation: SIP3E9F2A90AA06FB04
annotation: SIP199B1D0DBD4FD8CE
annotation: SIPBAF683526D045DCB
annotation: SIP8822C66A68EBF82C
annotation: SIPEC0B345AEC23487F
annotation: SIP10C7FA1892A3BE1A
annotation: SIPABFFC32EA4A62E7B
annotation: SIPCC0B4D01AEAB3AF7
annotation: SIPABD30E7A3632919B
annotation: SIP6DF349E7A735A301
annotation: SIP92208FB2D8D23E87
annotation: SIP2AC3B3D298CB6E13
annotation: SIP91984A2CA60D9F20
annotation: SIP33317973A2709BBC
annotation: SIP470826FCECA81D28
annotation: SIP74DDE6FFF95B3CC0
annotation: SIPE50D0F7D8F0D334E
annotation: SIP4CD439A1B525A9AC
annotation: SIP30D43F6B71FD6BDF
annotation: SIPA98A2ED943FF2ED9
annotation: SIP2578F3467E75FC1D
annotation: SIP5DADEAE0B15D7B8A
annotation: SIP7271B4032D1ABCA0
annotation: SIPCCA98F3678BD24BB
annotation: SIP9B8AA7C0F25ED132
annotation: SIPA284F787B719E436
annotation: SIP2A4F28A775DE40F1
annotation: SIPE6EB2FA8CC6249A7
annotation: SIP698BEA7B35D535E3
annotation: SIP6078D8BC014CB202
annotation: SIPD78D01E52E4974B4
annotation: SIP204D790009A36FC6
annotation: SIPA3A9E33367D2A881
annotation: SIPD7F67D25E809E95D
annotation: SIPD1FEBB19938CA09D
annotation: SIPB445EF92906F9923
annotation: SIP1169DEF9F0CD5308
annotation: SIP8C2B1CCC20B81AAA
annotation: SIP216D7C55DE41577A
annotation: SIP8E94957AC84FF3A7
annotation: SIP740398597D0CF523
annotation: SIP36279C6AC6034750
annotation: SIP94E711F0335D83B6
annotation: SIP45B8FD749BCD6DFF
annotation: SIP0F1930AF55EB64D9
annotation: SIP9E9F3B4160A559AF
annotation: SIPD53BBBF53340213C
annotation: SIPD71606D75A18C5A7
annotation: SIPB3C1810BB01F27A5
annotation: SIP06B6E36EEBC8B066
annotation: SIP0AF86FEC393367A6

P33160-citation-1954846
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

P33160-citation-2357236
type: Citation Statement
scope: PROTEIN SEQUENCE OF 2-394

P33160-citation-1597184
type: Citation Statement
scope: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS)

P33160-citation-12144528
type: Citation Statement
scope: CATALYTIC ACTIVITY
scope: BIOPHYSICOCHEMICAL PROPERTIES

P33160-citation-8484798
type: Citation Statement
scope: FUNCTION
scope: CATALYTIC ACTIVITY
scope: BIOPHYSICOCHEMICAL PROPERTIES
scope: MUTAGENESIS OF CYS-256

P33160-citation-8114093
type: Citation Statement
scope: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) OF 2-394 IN COMPLEX WITH NAD

P33160-citation-SIPCACDB8762C58F134
type: Citation Statement
scope: X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS)
scope: SUBUNIT

P33160-citation-SIP513C82697291997D
type: Citation Statement
scope: X-RAY CRYSTALLOGRAPHY (2.28 ANGSTROMS) IN COMPLEX WITH NAD AND FORMATE

2GO1
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.10
chain sequence mapping: PDB 2GO1 tt1tt401

PDB 2GO1 tt1tt401
chain: A=1-401

2GUG
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.28
chain sequence mapping: PDB 2GUG tt1tt401

PDB 2GUG tt1tt401
chain: A/B/C/D=1-401

2NAC
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 1.80
chain sequence mapping: PDB 2NAC tt2tt394

PDB 2NAC tt2tt394
chain: A/B=2-394

2NAD
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.05
chain sequence mapping: PDB 2NAD tt2tt394

PDB 2NAD tt2tt394
chain: A/B=2-394

6JUJ
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.18
chain sequence mapping: PDB 6JUJ tt1tt401

PDB 6JUJ tt1tt401
chain: A/B=1-401

6JUK
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.29
chain sequence mapping: PDB 6JUK tt1tt401

PDB 6JUK tt1tt401
chain: A/B=1-401

6JWG
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.08
chain sequence mapping: PDB 6JWG tt1tt401

PDB 6JWG tt1tt401
chain: A/B=1-401

6JX1
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.23
chain sequence mapping: PDB 6JX1 tt1tt401

PDB 6JX1 tt1tt401
chain: A/B=1-401

http://purl.uniprot.org/brenda/1.17.1.9
type: Resource
database: BRENDA
comment: 5085

cd05302
type: Resource
database: CDD
comment: FDH
signature sequence match: CDD cd05302 match 1

MF_03210
type: Resource
database: HAMAP
comment: Formate_dehydrogenase
signature sequence match: HAMAP MF 03210 match 1

IPR006139
type: Resource
database: InterPro
comment: D-isomer_2_OHA_DH_cat_dom

IPR029753
type: Resource
database: InterPro
comment: D-isomer_DH_CS

IPR029752
type: Resource
database: InterPro
comment: D-isomer_DH_CS1

IPR006140
type: Resource
database: InterPro
comment: D-isomer_DH_NAD-bd

IPR033689
type: Resource
database: InterPro
comment: FDH_NAD-dep

PTHR42938
type: Resource
database: PANTHER
comment: FORMATE DEHYDROGENASE 1
signature sequence match: PANTHER PTHR42938 match 1

PTHR42938:SF9
type: Resource
database: PANTHER
comment: FORMATE DEHYDROGENASE 1
signature sequence match: PANTHER PTHR42938:SF9 match 1

PF00389
type: Resource
database: Pfam
comment: 2-Hacid_dh
signature sequence match: Pfam PF00389 match 1

PF02826
type: Resource
database: Pfam
comment: 2-Hacid_dh_C
signature sequence match: Pfam PF02826 match 1

SSF52283
type: Resource
database: SUPFAM
comment: Formate/glycerate dehydrogenase catalytic domain-like
signature sequence match: SUPFAM SSF52283 match 1

PS00065
type: Resource
database: PROSITE
comment: D_2_HYDROXYACID_DH_1
signature sequence match: PROSITE PS00065 match 1

PS00670
type: Resource
database: PROSITE
comment: D_2_HYDROXYACID_DH_2
signature sequence match: PROSITE PS00670 match 1

PS00671
type: Resource
database: PROSITE
comment: D_2_HYDROXYACID_DH_3
signature sequence match: PROSITE PS00671 match 1

SIPD7FC11992EABA9EE
type: Structured Name
full name: Formate dehydrogenase
short name: FDH
ec name: 1.17.1.9

SIPCBFB018EED044498
type: Structured Name
full name: NAD-dependent formate dehydrogenase

SIPBEF5FB995478CD5A
type: Function Annotation
comment: Catalyzes the NAD(+)-dependent oxidation of formate to carbon dioxide. Formate oxidation is the final step in the methanol oxidation pathway in methylotrophic microorganisms. Has a role in the detoxification of exogenous formate in non-methylotrophic organisms.

SIPA340E0901765BE46
type: Kinetics Annotation
comment: kcat is 10 sec(-1) with formate as substrate.
measured affinity: 7.5 mM for formate
measured affinity: 7.0 mM for formate
measured affinity: 0.11 mM for NAD(+)
measured affinity: 60 uM for NAD(+)

SIP4CB576D98ACDA2AC
type: Subunit Annotation
comment: Homodimer.

SIP256FA1F2A44BC0B9
type: Similarity Annotation
comment: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. FDH subfamily.

SIP213D047F3CFD4169
type: Initiator Methionine Annotation
comment: Removed
range: 22574293098836014tt1tt1

22574293098836014tt1
type: Position
type: ExactPosition
position: 1
reference: P33160-1

PRO_0000076027
type: Chain Annotation
comment: Formate dehydrogenase
mass: 44005
range: 22574293098836014tt2tt401

22574293098836014tt2
type: Position
type: ExactPosition
position: 2
reference: P33160-1

22574293098836014tt401
type: Position
type: ExactPosition
position: 401
reference: P33160-1

22574293098836014tt123
type: Position
type: ExactPosition
position: 123
reference: P33160-1

22574293098836014tt147
type: Position
type: ExactPosition
position: 147
reference: P33160-1

22574293098836014tt148
type: Position
type: ExactPosition
position: 148
reference: P33160-1

22574293098836014tt202
type: Position
type: ExactPosition
position: 202
reference: P33160-1

22574293098836014tt203
type: Position
type: ExactPosition
position: 203
reference: P33160-1

22574293098836014tt222
type: Position
type: ExactPosition
position: 222
reference: P33160-1

22574293098836014tt257
type: Position
type: ExactPosition
position: 257
reference: P33160-1

22574293098836014tt261
type: Position
type: ExactPosition
position: 261
reference: P33160-1

22574293098836014tt283
type: Position
type: ExactPosition
position: 283
reference: P33160-1

22574293098836014tt309
type: Position
type: ExactPosition
position: 309
reference: P33160-1

22574293098836014tt333
type: Position
type: ExactPosition
position: 333
reference: P33160-1

22574293098836014tt336
type: Position
type: ExactPosition
position: 336
reference: P33160-1

22574293098836014tt381
type: Position
type: ExactPosition
position: 381
reference: P33160-1

SIP6DF349E7A735A301
type: Site Annotation
comment: Important for catalytic activity
range: 22574293098836014tt285tt285

22574293098836014tt285
type: Position
type: ExactPosition
position: 285
reference: P33160-1

SIP92208FB2D8D23E87
type: Site Annotation
comment: Important for catalytic activity
range: 22574293098836014tt333tt333

SIP2AC3B3D298CB6E13
type: Mutagenesis Annotation
comment: High resistance to inactivation by Hg(2+), Increased stability at 25 degree Celsius and decreased thermostability at 45 degree Celsius.
substitution: S
substitution: M
range: 22574293098836014tt256tt256

22574293098836014tt256
type: Position
type: ExactPosition
position: 256
reference: P33160-1

SIP91984A2CA60D9F20
type: Sequence Conflict Annotation
conflicting sequence: SIPE71BA964542D970A
substitution: S
range: 22574293098836014tt78tt78

22574293098836014tt78
type: Position
type: ExactPosition
position: 78
reference: P33160-1

SIP33317973A2709BBC
type: Sequence Conflict Annotation
conflicting sequence: SIPE71BA964542D970A
substitution: VT
range: 22574293098836014tt139tt140

22574293098836014tt139
type: Position
type: ExactPosition
position: 139
reference: P33160-1

22574293098836014tt140
type: Position
type: ExactPosition
position: 140
reference: P33160-1

SIP470826FCECA81D28
type: Sequence Conflict Annotation
conflicting sequence: SIPE71BA964542D970A
substitution: V
range: 22574293098836014tt146tt146

22574293098836014tt146
type: Position
type: ExactPosition
position: 146
reference: P33160-1

SIP74DDE6FFF95B3CC0
type: Sequence Conflict Annotation
conflicting sequence: SIPE71BA964542D970A
substitution: HV
range: 22574293098836014tt216tt217

22574293098836014tt216
type: Position
type: ExactPosition
position: 216
reference: P33160-1

22574293098836014tt217
type: Position
type: ExactPosition
position: 217
reference: P33160-1

SIPE50D0F7D8F0D334E
type: Sequence Conflict Annotation
conflicting sequence: SIPE71BA964542D970A
substitution: D
range: 22574293098836014tt328tt328

22574293098836014tt328
type: Position
type: ExactPosition
position: 328
reference: P33160-1

22574293098836014tt3
type: Position
type: ExactPosition
position: 3
reference: P33160-1

22574293098836014tt7
type: Position
type: ExactPosition
position: 7
reference: P33160-1

22574293098836014tt21
type: Position
type: ExactPosition
position: 21
reference: P33160-1

22574293098836014tt23
type: Position
type: ExactPosition
position: 23
reference: P33160-1

22574293098836014tt54
type: Position
type: ExactPosition
position: 54
reference: P33160-1

22574293098836014tt56
type: Position
type: ExactPosition
position: 56
reference: P33160-1

22574293098836014tt57
type: Position
type: ExactPosition
position: 57
reference: P33160-1

22574293098836014tt59
type: Position
type: ExactPosition
position: 59
reference: P33160-1

22574293098836014tt61
type: Position
type: ExactPosition
position: 61
reference: P33160-1

22574293098836014tt66
type: Position
type: ExactPosition
position: 66
reference: P33160-1

22574293098836014tt70
type: Position
type: ExactPosition
position: 70
reference: P33160-1

22574293098836014tt75
type: Position
type: ExactPosition
position: 75
reference: P33160-1

22574293098836014tt83
type: Position
type: ExactPosition
position: 83
reference: P33160-1

22574293098836014tt88
type: Position
type: ExactPosition
position: 88
reference: P33160-1

22574293098836014tt92
type: Position
type: ExactPosition
position: 92
reference: P33160-1

22574293098836014tt97
type: Position
type: ExactPosition
position: 97
reference: P33160-1

22574293098836014tt106
type: Position
type: ExactPosition
position: 106
reference: P33160-1

22574293098836014tt111
type: Position
type: ExactPosition
position: 111
reference: P33160-1

22574293098836014tt117
type: Position
type: ExactPosition
position: 117
reference: P33160-1

22574293098836014tt130
type: Position
type: ExactPosition
position: 130
reference: P33160-1

22574293098836014tt135
type: Position
type: ExactPosition
position: 135
reference: P33160-1

22574293098836014tt142
type: Position
type: ExactPosition
position: 142
reference: P33160-1

22574293098836014tt144
type: Position
type: ExactPosition
position: 144
reference: P33160-1

22574293098836014tt163
type: Position
type: ExactPosition
position: 163
reference: P33160-1

22574293098836014tt166
type: Position
type: ExactPosition
position: 166
reference: P33160-1

22574293098836014tt174
type: Position
type: ExactPosition
position: 174
reference: P33160-1

22574293098836014tt180
type: Position
type: ExactPosition
position: 180
reference: P33160-1

22574293098836014tt184
type: Position
type: ExactPosition
position: 184
reference: P33160-1

22574293098836014tt194
type: Position
type: ExactPosition
position: 194
reference: P33160-1

22574293098836014tt198
type: Position
type: ExactPosition
position: 198
reference: P33160-1

22574293098836014tt211
type: Position
type: ExactPosition
position: 211
reference: P33160-1

22574293098836014tt212
type: Position
type: ExactPosition
position: 212
reference: P33160-1

22574293098836014tt214
type: Position
type: ExactPosition
position: 214
reference: P33160-1

22574293098836014tt221
type: Position
type: ExactPosition
position: 221
reference: P33160-1

22574293098836014tt228
type: Position
type: ExactPosition
position: 228
reference: P33160-1

22574293098836014tt234
type: Position
type: ExactPosition
position: 234
reference: P33160-1

22574293098836014tt237
type: Position
type: ExactPosition
position: 237
reference: P33160-1

22574293098836014tt241
type: Position
type: ExactPosition
position: 241
reference: P33160-1

22574293098836014tt242
type: Position
type: ExactPosition
position: 242
reference: P33160-1

22574293098836014tt245
type: Position
type: ExactPosition
position: 245
reference: P33160-1

22574293098836014tt246
type: Position
type: ExactPosition
position: 246
reference: P33160-1

22574293098836014tt248
type: Position
type: ExactPosition
position: 248
reference: P33160-1

22574293098836014tt250
type: Position
type: ExactPosition
position: 250
reference: P33160-1

22574293098836014tt254
type: Position
type: ExactPosition
position: 254
reference: P33160-1

22574293098836014tt260
type: Position
type: ExactPosition
position: 260
reference: P33160-1

22574293098836014tt264
type: Position
type: ExactPosition
position: 264
reference: P33160-1

22574293098836014tt265
type: Position
type: ExactPosition
position: 265
reference: P33160-1

22574293098836014tt267
type: Position
type: ExactPosition
position: 267
reference: P33160-1

22574293098836014tt268
type: Position
type: ExactPosition
position: 268
reference: P33160-1

22574293098836014tt271
type: Position
type: ExactPosition
position: 271
reference: P33160-1

22574293098836014tt278
type: Position
type: ExactPosition
position: 278
reference: P33160-1

22574293098836014tt282
type: Position
type: ExactPosition
position: 282
reference: P33160-1

22574293098836014tt286
type: Position
type: ExactPosition
position: 286
reference: P33160-1

22574293098836014tt288
type: Position
type: ExactPosition
position: 288
reference: P33160-1

22574293098836014tt291
type: Position
type: ExactPosition
position: 291
reference: P33160-1

22574293098836014tt299
type: Position
type: ExactPosition
position: 299
reference: P33160-1

22574293098836014tt302
type: Position
type: ExactPosition
position: 302
reference: P33160-1

22574293098836014tt312
type: Position
type: ExactPosition
position: 312
reference: P33160-1

22574293098836014tt315
type: Position
type: ExactPosition
position: 315
reference: P33160-1

22574293098836014tt321
type: Position
type: ExactPosition
position: 321
reference: P33160-1

22574293098836014tt323
type: Position
type: ExactPosition
position: 323
reference: P33160-1

22574293098836014tt335
type: Position
type: ExactPosition
position: 335
reference: P33160-1

22574293098836014tt337
type: Position
type: ExactPosition
position: 337
reference: P33160-1

22574293098836014tt339
type: Position
type: ExactPosition
position: 339
reference: P33160-1

22574293098836014tt358
type: Position
type: ExactPosition
position: 358
reference: P33160-1

22574293098836014tt364
type: Position
type: ExactPosition
position: 364
reference: P33160-1

22574293098836014tt366
type: Position
type: ExactPosition
position: 366
reference: P33160-1

22574293098836014tt367
type: Position
type: ExactPosition
position: 367
reference: P33160-1

22574293098836014tt370
type: Position
type: ExactPosition
position: 370
reference: P33160-1

22574293098836014tt371
type: Position
type: ExactPosition
position: 371
reference: P33160-1

22574293098836014tt373
type: Position
type: ExactPosition
position: 373
reference: P33160-1

22574293098836014tt377
type: Position
type: ExactPosition
position: 377
reference: P33160-1

substrate

P33160-1
type: Simple Sequence
modified: 2007-01-23
version: 3
mass: 44136
value: MAKVLCVLYDDPVDGYPKTYARDDLPKIDHYPGGQTLPTPKAIDFTPGQLLGSVSGELGLRKYLESNGHTLVVTSDKDGPDSVFERELVDADVVISQPFWPAYLTPERIAKAKNLKLALTAGIGSDHVDLQSAIDRNVTVAEVTYCNSISVAEHVVMMILSLVRNYLPSHEWARKGGWNIADCVSHAYDLEAMHVGTVAAGRIGLAVLRRLAPFDVHLHYTDRHRLPESVEKELNLTWHATREDMYPVCDVVTLNCPLHPETEHMINDETLKLFKRGAYIVNTARGKLCDRDAVARALESGRLAGYAGDVWFPQPAPKDHPWRTMPYNGMTPHISGTTLTAQARYAAGTREILECFFEGRPIRDEYLIVQGGALAGTGAHSYSKGNATGGSEEAAKFKKAV

http://purl.uniprot.org/uniprot/P33160#attribution-C3F9B66FA54A1C34D5B62D8EB56D0EE5
manual: true
evidence: ECO_0000255
source: MF_03210

http://purl.uniprot.org/uniprot/P33160#attribution-F64D3F566334650ED4E5D7B5DD5894EC
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/12144528

http://purl.uniprot.org/uniprot/P33160#attribution-D584BC8BB92235959E38F2E2B5A3B282
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/2357236

http://purl.uniprot.org/uniprot/P33160#attribution-EDDF4C4EC4D6932B657EE042C7E2917D
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/8114093

http://purl.uniprot.org/uniprot/P33160#attribution-257745979ACA1A39F33BE0B079B58539
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/8484798

http://purl.uniprot.org/uniprot/P33160#attribution-08D64494A5D3460DA2D5D186C7C1B869
manual: true
evidence: ECO_0000269
source: SIPCACDB8762C58F134

http://purl.uniprot.org/uniprot/P33160#attribution-31F613BC51565631919606A75CC86BA0
manual: true
evidence: ECO_0000269
source: SIP513C82697291997D

http://purl.uniprot.org/uniprot/P33160#attribution-349B3697E6BF3725825DB0C16BC35470
manual: true
evidence: ECO_0000303
source: http://purl.uniprot.org/citations/1954846

http://purl.uniprot.org/uniprot/P33160#attribution-0DACBCD2EC4C6C27AA03C6741749D81E
manual: true
evidence: ECO_0000303
source: http://purl.uniprot.org/citations/8484798

http://purl.uniprot.org/uniprot/P33160#attribution-080ED3982FEC43F7C43A0689717F3D20
manual: true
evidence: ECO_0000305

http://purl.uniprot.org/uniprot/P33160#attribution-B601E629E7E9A46563D5173F05A1505E
manual: true
evidence: ECO_0000305
source: http://purl.uniprot.org/citations/8114093

P36267
type: Protein
reviewed: true
created: 1994-06-01
modified: 2023-09-13
version: 83
mnemonic: GGT_PSEUA
old mnemonic: GGT_PSESP
citation: http://purl.uniprot.org/citations/7765305
recommended name: SIP5814DD39DF5FDD94
alternative name: SIPE62BBD2764217A68
enzyme: http://purl.uniprot.org/enzyme/3.4.19.13
enzyme: http://purl.uniprot.org/enzyme/2.3.2.2
component: SIP56E7B0811389079B
component: SIP07175B9753FDCF75
organism: http://purl.uniprot.org/taxonomy/69010
annotation: SIP3C2D4D10BA2A5884
annotation: SIP387D58BD49F20918
annotation: SIP625D282780B9AB96
annotation: SIPA12E158C1440B38E
annotation: SIP4C4A65CACB4602E5
annotation: SIPE8E3032E680C3728
annotation: SIPBBE97F990C39ADD5
annotation: SIP0B88E58CE8099945
annotation: SIPA572E605BF5FEC06
annotation: PRO_0000011054
annotation: PRO_0000011055
annotation: SIPE5383BB95AFDCC8F
annotation: SIP67E6A646BF0B8D2A
annotation: SIPD459C8773B986726
annotation: SIP653400F582A7ADB8
annotation: SIPDE03465C0F98D1D6
annotation: SIPC43A9A2E411C6876
annotation: SIPEFD19B83CB2EBF52
annotation: SIPE07500F756F9DD8F
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/12
classified with: http://purl.uniprot.org/keywords/903
classified with: http://purl.uniprot.org/keywords/317
classified with: http://purl.uniprot.org/keywords/574
classified with: http://purl.uniprot.org/keywords/645
classified with: http://purl.uniprot.org/keywords/732
classified with: http://purl.uniprot.org/keywords/865
classified with: GO_0042597
classified with: GO_0036374
classified with: GO_0102953
classified with: GO_0103068
classified with: GO_0006750
classified with: GO_0006751
classified with: GO_0006508
sequence: P36267-1

P36267-citation-7765305
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
scope: PARTIAL PROTEIN SEQUENCE
scope: CATALYTIC ACTIVITY
scope: SUBUNIT
scope: AUTOCATALYTIC CLEAVAGE

A14
type: Strain

IPR043138
type: Resource
database: InterPro
comment: GGT_lsub_C

IPR000101
type: Resource
database: InterPro
comment: GGT_peptidase

IPR043137
type: Resource
database: InterPro
comment: GGT_ssub

IPR029055
type: Resource
database: InterPro
comment: Ntn_hydrolases_N

TIGR00066
type: Resource
database: NCBIfam
comment: g_glut_trans
signature sequence match: NCBIfam TIGR00066 match 1

PTHR43199
type: Resource
database: PANTHER
comment: GLUTATHIONE HYDROLASE
signature sequence match: PANTHER PTHR43199 match 1

PTHR43199:SF1
type: Resource
database: PANTHER
comment: GLUTATHIONE HYDROLASE PROENZYME
signature sequence match: PANTHER PTHR43199:SF1 match 1

PF01019
type: Resource
database: Pfam
comment: G_glu_transpept
signature sequence match: Pfam PF01019 match 1

PR01210
type: Resource
database: PRINTS
comment: GGTRANSPTASE

SSF56235
type: Resource
database: SUPFAM
comment: N-terminal nucleophile aminohydrolases (Ntn hydrolases)
signature sequence match: SUPFAM SSF56235 match 1

PS00462
type: Resource
database: PROSITE
comment: G_GLU_TRANSPEPTIDASE
signature sequence match: PROSITE PS00462 match 1

SIP5814DD39DF5FDD94
type: Structured Name
full name: Glutathione hydrolase proenzyme
ec name: 3.4.19.13

SIPE62BBD2764217A68
type: Structured Name
full name: Gamma-glutamyltranspeptidase proenzyme
ec name: 2.3.2.2

SIPF255954FA6753FF9
type: Structured Name
full name: Glutathione hydrolase large chain

SIP4CAE9024EB378157
type: Structured Name
full name: Glutathione hydrolase small chain

ggt
type: Gene

SIP4C4A65CACB4602E5
type: Subunit Annotation
comment: This enzyme consists of two polypeptide chains, which are synthesized in precursor form from a single polypeptide.

SIPBBE97F990C39ADD5
type: PTM Annotation
comment: Cleaved by autocatalysis into a large and a small subunit.

SIP0B88E58CE8099945
type: Similarity Annotation
comment: Belongs to the gamma-glutamyltransferase family.

22574306000516910tt1
type: Position
type: ExactPosition
position: 1
reference: P36267-1

22574306000516910tt24
type: Position
type: ExactPosition
position: 24
reference: P36267-1

PRO_0000011054
type: Chain Annotation
comment: Glutathione hydrolase large chain
mass: 37356
range: 22574306000516910tt25tt375

22574306000516910tt25
type: Position
type: ExactPosition
position: 25
reference: P36267-1

22574306000516910tt375
type: Position
type: ExactPosition
position: 375
reference: P36267-1

PRO_0000011055
type: Chain Annotation
comment: Glutathione hydrolase small chain
mass: 21401
range: 22574306000516910tt376tt575

22574306000516910tt376
type: Position
type: ExactPosition
position: 376
reference: P36267-1

22574306000516910tt575
type: Position
type: ExactPosition
position: 575
reference: P36267-1

SIPE5383BB95AFDCC8F
type: Active Site Annotation
comment: Nucleophile
range: 22574306000516910tt376tt376

22574306000516910tt100
type: Position
type: ExactPosition
position: 100
reference: P36267-1

22574306000516910tt394
type: Position
type: ExactPosition
position: 394
reference: P36267-1

22574306000516910tt396
type: Position
type: ExactPosition
position: 396
reference: P36267-1

22574306000516910tt415
type: Position
type: ExactPosition
position: 415
reference: P36267-1

22574306000516910tt418
type: Position
type: ExactPosition
position: 418
reference: P36267-1

22574306000516910tt447
type: Position
type: ExactPosition
position: 447
reference: P36267-1

22574306000516910tt448
type: Position
type: ExactPosition
position: 448
reference: P36267-1

22574306000516910tt468
type: Position
type: ExactPosition
position: 468
reference: P36267-1

22574306000516910tt469
type: Position
type: ExactPosition
position: 469
reference: P36267-1

P36267-1
type: Simple Sequence
modified: 1994-06-01
version: 1
precursor: true
mass: 61301
value: MKNQTFSKALLATALSCALFNVHAASQAPVGAENGMVVTAQHIASKVGVEVLKSGGNAIDAAVAVGYALAVVYPAAGNIGGGGFMTIQLADGRKTFLDFREKAPLAATANMYLDKDGNVIKGASTTGYLAVGVPGTVSGMEYAREKYGTKTRQQLISPAITLADKGFVLEQGDVDMLWTSTKDFEKDRANSGAIFMNKGQPFQPGERLVQKDLARTLRLISAKGTDGFYKGEVADKLVASMKAGGGIITQADLDQYKTRELAPVECDYRGYHVVSAPPPSSGGVVICEIMNILEGYPMKELGYHSAQGVHYTIEAMRHAYVDRNSYLGDPDFVKNPLAHLLDKDYAAKIRAAINPQKAGISQEIKPGVPPHEGSNTTHYSIVDKDGNAVSVTYTLNDWFGAKVMANGTGVLLNDEMDDFTSKVGVPNMYGLIQGEANAIGPGRRPLSSMSPTIVTKDGKTVMVVGTPGGSRIITATLLTMLNMIDYGMNLQEAVDAPRFHQQWMPESTNIEAFALSPDTQKILESWGQKFAGPQPANHIAAILVGAPSLGGKPIGKNRFYGANDPRRNTGLALGY

http://purl.uniprot.org/uniprot/P36267#attribution-609619A7DF3E53CE5E48EDEF8B8F9DD1
manual: true
evidence: ECO_0000250

http://purl.uniprot.org/uniprot/P36267#attribution-366416A3EDC37B265327D6A3A8BCE673
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/7765305

http://purl.uniprot.org/uniprot/P36267#attribution-080ED3982FEC43F7C43A0689717F3D20
manual: true
evidence: ECO_0000305

P82997
type: Protein
reviewed: true
created: 2004-05-10
modified: 2023-05-03
version: 31
mnemonic: GSTE_PSEUF
old mnemonic: GTE_PSEUF
citation: http://purl.uniprot.org/citations/11900268
recommended name: SIP2A4294605FDC2406
enzyme: http://purl.uniprot.org/enzyme/2.5.1.18
organism: http://purl.uniprot.org/taxonomy/79676
annotation: SIPC1DC1D45F5235D17
annotation: SIPDAFB0604EA4DC8F6
annotation: SIP22FEDCB7F66BB530
annotation: SIPB606D9C50E0EF888
annotation: SIP07CA1416383E2841
annotation: PRO_0000185978
annotation: SIP518187E35C982FD0
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/963
classified with: http://purl.uniprot.org/keywords/903
classified with: http://purl.uniprot.org/keywords/808
classified with: GO_0005737
classified with: GO_0004364
sequence: MLKLHGFSVSNYYNMVKLALLEKG

P82997-citation-11900268
type: Citation Statement
scope: PROTEIN SEQUENCE
scope: FUNCTION
scope: CATALYTIC ACTIVITY
scope: SUBUNIT
scope: SUBCELLULAR LOCATION

SIP2A4294605FDC2406
type: Structured Name
full name: Glutathione S-transferase
ec name: 2.5.1.18

SIPC1DC1D45F5235D17
type: Function Annotation
comment: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.

SIP22FEDCB7F66BB530
type: Subunit Annotation
comment: Monomer and homodimer.

SIP07CA1416383E2841
type: Similarity Annotation
comment: Belongs to the GST superfamily.

PRO_0000185978
type: Chain Annotation
comment: Glutathione S-transferase
mass: 2756
range: 22579786496423726tt1tf24

22579786496423726tt1
type: Position
type: ExactPosition
position: 1
reference: MLKLHGFSVSNYYNMVKLALLEKG

22579786496423726tt24
type: Position
type: ExactPosition
position: 24
reference: MLKLHGFSVSNYYNMVKLALLEKG

MLKLHGFSVSNYYNMVKLALLEKG
type: Simple Sequence
modified: 2002-03-01
version: 1
fragment: single
mass: 2756

http://purl.uniprot.org/uniprot/P82997#attribution-437CC58AF65A3367D07CB6E0538365AC
creator: UniProtKB
manual: true
evidence: ECO_0000303
source: http://purl.uniprot.org/citations/11900268

http://purl.uniprot.org/uniprot/P82997#attribution-A12D7194EF673A940D19340690B1D9AE
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/11900268

http://purl.uniprot.org/uniprot/P82997#attribution-B36C7F24D1A634934DC08F9F896036BD
manual: true
evidence: ECO_0000303
source: http://purl.uniprot.org/citations/11900268

http://purl.uniprot.org/uniprot/P82997#attribution-4DF7F78D37E1C5CA36CDA4F3B6EB0DDC
manual: true
evidence: ECO_0000305

P24069
type: Protein
reviewed: true
created: 1992-03-01
modified: 2023-09-13
version: 80
mnemonic: HAD1_PSEUC
old mnemonic: HAD1_PSESP
citation: http://purl.uniprot.org/citations/1995594
recommended name: SIPBCF280C9ECE26528
alternative name: SIP63767CB22D48ED3D
alternative name: SIP00ED9F2D368342D3
alternative name: SIPE864E0A5CB971F9C
alternative name: SIP4FD3C1FA7731F2B4
enzyme: http://purl.uniprot.org/enzyme/3.8.1.2
organism: http://purl.uniprot.org/taxonomy/72586
annotation: SIPF888C3CB7EDB976A
annotation: SIPFD6FC817FE26F984
annotation: SIP59BF16A88F7E296A
annotation: SIP934417CE378AC96C
annotation: SIP2C28AD910C9F2035
annotation: PRO_0000079160
annotation: SIPF5A383A1D0E44053
annotation: SIP33ED2F8CEF75E770
annotation: SIP9B63A1AEC4A786D8
annotation: SIPA818625833BCD43D
annotation: SIPEA838ED6698AF756
annotation: SIPF82244DCAB977CC9
annotation: SIPAF38404A099631B9
annotation: SIP6DB9AA88120CD0EB
existence: Inferred from Homology Existence
classified with: http://purl.uniprot.org/keywords/378
classified with: GO_0018784
sequence: P24069-1

P24069-citation-1995594
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

cd02588
type: Resource
database: CDD
comment: HAD_L2-DEX
signature sequence match: CDD cd02588 match 1

http://purl.uniprot.org/gene3d/3.40.50.1000
type: Resource
database: Gene3D
comment: HAD superfamily/HAD-like
signature sequence match: Gene3D 3.40.50.1000 match 1

IPR006328
type: Resource
database: InterPro
comment: 2-HAD

IPR036412
type: Resource
database: InterPro
comment: HAD-like_sf

IPR006439
type: Resource
database: InterPro
comment: HAD-SF_hydro_IA

IPR023214
type: Resource
database: InterPro
comment: HAD_sf

IPR023198
type: Resource
database: InterPro
comment: PGP-like_dom2

TIGR01493
type: Resource
database: NCBIfam
comment: HAD-SF-IA-v2
signature sequence match: NCBIfam TIGR01493 match 1

TIGR01509
type: Resource
database: NCBIfam
comment: HAD-SF-IA-v3
signature sequence match: NCBIfam TIGR01509 match 1

TIGR01428
type: Resource
database: NCBIfam
comment: HAD_type_II
signature sequence match: NCBIfam TIGR01428 match 1

PTHR43316:SF3
type: Resource
database: PANTHER
comment: HALOACID DEHALOGENASE, TYPE II (AFU_ORTHOLOGUE AFUA_2G07750)-RELATED
signature sequence match: PANTHER PTHR43316:SF3 match 1

PTHR43316
type: Resource
database: PANTHER
comment: HYDROLASE, HALOACID DELAHOGENASE-RELATED
signature sequence match: PANTHER PTHR43316 match 1

PF00702
type: Resource
database: Pfam
comment: Hydrolase
signature sequence match: Pfam PF00702 match 1

PR00413
type: Resource
database: PRINTS
comment: HADHALOGNASE

SFLDF00045
type: Resource
database: SFLD
comment: 2-haloacid_dehalogenase
signature sequence match: SFLD SFLDF00045 match 1

SFLDS00003
type: Resource
database: SFLD
comment: Haloacid_Dehalogenase
signature sequence match: SFLD SFLDS00003 match 1

SSF56784
type: Resource
database: SUPFAM
comment: HAD-like
signature sequence match: SUPFAM SSF56784 match 1

SIPBCF280C9ECE26528
type: Structured Name
full name: (S)-2-haloacid dehalogenase 1
ec name: 3.8.1.2

SIP63767CB22D48ED3D
type: Structured Name
full name: 2-haloalkanoic acid dehalogenase I

SIP00ED9F2D368342D3
type: Structured Name
full name: DEHCI

SIPE864E0A5CB971F9C
type: Structured Name
full name: Halocarboxylic acid halidohydrolase I

SIP4FD3C1FA7731F2B4
type: Structured Name
full name: L-2-haloacid dehalogenase I

SIPF888C3CB7EDB976A
type: Function Annotation
comment: Catalyzes the hydrolytic dehalogenation of small (S)-2-haloalkanoic acids to yield the corresponding (R)-2-hydroxyalkanoic acids. Acts on acids of short chain lengths, C(2) to C(4), with inversion of configuration at C-2. Active with 2-halogenated carboxylic acids and converts only the S-isomer (or L-isomer) of 2-chloropropionic acid with inversion of configuration to produce R-lactate (or D-isomer).

SIP934417CE378AC96C
type: Biotechnology Annotation
comment: (S)-2-haloacid dehalogenases may be used for the biodegradation of halogenated substances and their derivatives which are widely used as pesticides, herbicides and other industrial products.

SIP2C28AD910C9F2035
type: Similarity Annotation
comment: Belongs to the HAD-like hydrolase superfamily. S-2-haloalkanoic acid dehalogenase family.

PRO_0000079160
type: Chain Annotation
comment: (S)-2-haloacid dehalogenase 1
mass: 25430
range: 22573197865400622tt1tt227

22573197865400622tt1
type: Position
type: ExactPosition
position: 1
reference: P24069-1

22573197865400622tt227
type: Position
type: ExactPosition
position: 227
reference: P24069-1

SIPF5A383A1D0E44053
type: Region Annotation
comment: Important for catalytic activity
range: 22573197865400622tt175tt180

22573197865400622tt175
type: Position
type: ExactPosition
position: 175
reference: P24069-1

22573197865400622tt180
type: Position
type: ExactPosition
position: 180
reference: P24069-1

SIP33ED2F8CEF75E770
type: Active Site Annotation
comment: Nucleophile
range: 22573197865400622tt10tt10

22573197865400622tt10
type: Position
type: ExactPosition
position: 10
reference: P24069-1

22573197865400622tt11
type: Position
type: ExactPosition
position: 11
reference: P24069-1

22573197865400622tt12
type: Position
type: ExactPosition
position: 12
reference: P24069-1

22573197865400622tt41
type: Position
type: ExactPosition
position: 41
reference: P24069-1

22573197865400622tt118
type: Position
type: ExactPosition
position: 118
reference: P24069-1

22573197865400622tt119
type: Position
type: ExactPosition
position: 119
reference: P24069-1

SIPF82244DCAB977CC9
type: Site Annotation
comment: Important for catalytic activity
range: 22573197865400622tt14tt14

22573197865400622tt14
type: Position
type: ExactPosition
position: 14
reference: P24069-1

SIPAF38404A099631B9
type: Site Annotation
comment: Important for catalytic activity
range: 22573197865400622tt151tt151

22573197865400622tt151
type: Position
type: ExactPosition
position: 151
reference: P24069-1

SIP6DB9AA88120CD0EB
type: Site Annotation
comment: Important for catalytic activity
range: 22573197865400622tt157tt157

22573197865400622tt157
type: Position
type: ExactPosition
position: 157
reference: P24069-1

P24069-1
type: Simple Sequence
modified: 1993-10-01
version: 2
mass: 25430
value: MDPIRACVFDAYGTLLDVNTAVMKHAHDIGGCAEELSSLWRQRQLEYSWTRTLMGRYADFWQLTTEALDFALESFGLLERTDLKNRLLDAYHELSAYPDAVGTLGALKAAGFTTAILSNGNNEMLRGALRAGNLTEALDQCISVDEIKIYKPDPRVYQFACDRLDVRPSEVCFVSSNAWDIGGAGAFGFNTVRINRINKPQEYSFAPQRHQLSSLSELPQLLLRLTQ

http://purl.uniprot.org/uniprot/P24069#attribution-2D9B71DEEB9ACEBEC646A8E8FC2CD453
manual: true
evidence: ECO_0000250
source: Q53464

http://purl.uniprot.org/uniprot/P24069#attribution-D50559789A995BB19F56570F25C417B1
manual: true
evidence: ECO_0000303
source: http://purl.uniprot.org/citations/1995594

http://purl.uniprot.org/uniprot/P24069#attribution-F056FFA9203390C71E154C607B49960E
manual: true
evidence: ECO_0000305

P24070
type: Protein
reviewed: true
created: 1992-03-01
modified: 2023-09-13
version: 77
mnemonic: HAD2_PSEUC
old mnemonic: HAD2_PSESP
citation: http://purl.uniprot.org/citations/1995594
recommended name: SIP58D89DAEAC9D127E
alternative name: SIP96D6D729521FA318
alternative name: SIP4E8863489CBC5255
alternative name: SIP57687814DE4C9320
alternative name: SIPB86D39376C7C1F0B
enzyme: http://purl.uniprot.org/enzyme/3.8.1.2
organism: http://purl.uniprot.org/taxonomy/72586
annotation: SIP04298C9B2E5E6E44
annotation: SIPB62390534F189146
annotation: SIP446F6B4FDAEA239B
annotation: SIPABE506F8A7E387B3
annotation: SIPD3721E6771530C3A
annotation: PRO_0000079161
annotation: SIPDCF21D58F4DD003F
annotation: SIPCA9DE1809B9F3B34
annotation: SIP789C2326AEA655E2
annotation: SIP3AB39ECDBE92F892
annotation: SIP85A3E57D2C2D3A3B
annotation: SIP4CFC55BB328AA203
annotation: SIP7D917155B11A2F9D
annotation: SIPF4C2D90F9B1A8C41
existence: Inferred from Homology Existence
classified with: http://purl.uniprot.org/keywords/378
classified with: GO_0018784
sequence: P24070-1

P24070-citation-1995594
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

IPR041492
type: Resource
database: InterPro
comment: HAD_2

TIGR01549
type: Resource
database: NCBIfam
comment: HAD-SF-IA-v1
signature sequence match: NCBIfam TIGR01549 match 1

PF13419
type: Resource
database: Pfam
comment: HAD_2
signature sequence match: Pfam PF13419 match 1

SFLDG01135
type: Resource
database: SFLD
comment: C1.5.6:_HAD__Beta-PGM__Phospha
signature sequence match: SFLD SFLDG01135 match 1

SIP58D89DAEAC9D127E
type: Structured Name
full name: (S)-2-haloacid dehalogenase 2
ec name: 3.8.1.2

SIP96D6D729521FA318
type: Structured Name
full name: 2-haloalkanoic acid dehalogenase II

SIP4E8863489CBC5255
type: Structured Name
full name: DEHCII

SIP57687814DE4C9320
type: Structured Name
full name: Halocarboxylic acid halidohydrolase II

SIPB86D39376C7C1F0B
type: Structured Name
full name: L-2-haloacid dehalogenase II

SIP04298C9B2E5E6E44
type: Function Annotation
comment: Catalyzes the hydrolytic dehalogenation of small (S)-2-haloalkanoic acids to yield the corresponding (R)-2-hydroxyalkanoic acids. Acts on acids of short chain lengths, C(2) to C(4), with inversion of configuration at C-2. Active with 2-halogenated carboxylic acids and converts only the S-isomer (or L-isomer) of 2-chloropropionic acid with inversion of configuration to produce R-lactate (or D-isomer).

SIPABE506F8A7E387B3
type: Biotechnology Annotation
comment: (S)-2-haloacid dehalogenases may be used for the biodegradation of halogenated substances and their derivatives which are widely used as pesticides, herbicides and other industrial products.

SIPD3721E6771530C3A
type: Similarity Annotation
comment: Belongs to the HAD-like hydrolase superfamily. S-2-haloalkanoic acid dehalogenase family.

PRO_0000079161
type: Chain Annotation
comment: (S)-2-haloacid dehalogenase 2
mass: 25712
range: 22573197865463854tt1tt229

22573197865463854tt1
type: Position
type: ExactPosition
position: 1
reference: P24070-1

22573197865463854tt229
type: Position
type: ExactPosition
position: 229
reference: P24070-1

SIPDCF21D58F4DD003F
type: Region Annotation
comment: Important for catalytic activity
range: 22573197865463854tt175tt180

22573197865463854tt175
type: Position
type: ExactPosition
position: 175
reference: P24070-1

22573197865463854tt180
type: Position
type: ExactPosition
position: 180
reference: P24070-1

SIPCA9DE1809B9F3B34
type: Active Site Annotation
comment: Nucleophile
range: 22573197865463854tt10tt10

22573197865463854tt10
type: Position
type: ExactPosition
position: 10
reference: P24070-1

22573197865463854tt11
type: Position
type: ExactPosition
position: 11
reference: P24070-1

22573197865463854tt12
type: Position
type: ExactPosition
position: 12
reference: P24070-1

22573197865463854tt41
type: Position
type: ExactPosition
position: 41
reference: P24070-1

22573197865463854tt118
type: Position
type: ExactPosition
position: 118
reference: P24070-1

22573197865463854tt119
type: Position
type: ExactPosition
position: 119
reference: P24070-1

SIP4CFC55BB328AA203
type: Site Annotation
comment: Important for catalytic activity
range: 22573197865463854tt14tt14

22573197865463854tt14
type: Position
type: ExactPosition
position: 14
reference: P24070-1

SIP7D917155B11A2F9D
type: Site Annotation
comment: Important for catalytic activity
range: 22573197865463854tt151tt151

22573197865463854tt151
type: Position
type: ExactPosition
position: 151
reference: P24070-1

SIPF4C2D90F9B1A8C41
type: Site Annotation
comment: Important for catalytic activity
range: 22573197865463854tt157tt157

22573197865463854tt157
type: Position
type: ExactPosition
position: 157
reference: P24070-1

P24070-1
type: Simple Sequence
modified: 1992-03-01
version: 1
mass: 25712
value: MQEIRGVVFDLYGTLCDVHSVAQLCGQYFPERGTEISLMWRQKQLEYSWLRSLMGQYVSFPQATEDALVFVCNALNLKLREDTRIALCNEYLNIKPYREVRSALESLRSGAVPLAILSNGSAHSIQSVVGNAGIEHFFSHLISADEVSVSKPSPAAYELAEKRLKVVRSKLLFVSSNAWDASGARHFGFQVCWVNRSRNTFEQLGERPDHVISGLDELPNLLNFASADR

http://purl.uniprot.org/uniprot/P24070#attribution-2D9B71DEEB9ACEBEC646A8E8FC2CD453
manual: true
evidence: ECO_0000250
source: Q53464

http://purl.uniprot.org/uniprot/P24070#attribution-D50559789A995BB19F56570F25C417B1
manual: true
evidence: ECO_0000303
source: http://purl.uniprot.org/citations/1995594

http://purl.uniprot.org/uniprot/P24070#attribution-F056FFA9203390C71E154C607B49960E
manual: true
evidence: ECO_0000305

O06652
type: Protein
reviewed: true
created: 2004-03-01
modified: 2023-05-03
version: 52
mnemonic: HADDL_PSES4
old mnemonic: HDDL_PSES4
citation: http://purl.uniprot.org/citations/9209038
recommended name: SIP3972A79D22EABEE8
alternative name: SIP698768577824A62E
alternative name: SIP83BDDFF6849C8FC4
enzyme: http://purl.uniprot.org/enzyme/3.8.1.10
organism: http://purl.uniprot.org/taxonomy/59918
annotation: SIPAE7D1156F3EE2DD1
annotation: SIP2169EE3646B07673
annotation: SIPB8E43B68173418D0
annotation: SIPF891322D8A73E1FE
annotation: SIPEA6A7672F2E642AA
annotation: PRO_0000079172
annotation: SIP6F80A3EBB7BD250C
annotation: SIPA9CED1A980B90C0B
annotation: SIPBFFD5D4E674E20EF
annotation: SIP3ED21539FB40A723
annotation: SIPC230AC0A148FB4E6
annotation: SIP8A43AD98A46B52A9
annotation: SIP5727C0291424F173
annotation: SIP736002E08D5614A0
annotation: SIP2FB3AF9162E23F10
annotation: SIP7CFC3C6D7271EB51
annotation: SIP32C299D00551310F
annotation: SIPA41AC780AC7FCA78
annotation: SIPBF83325BCE1C5AD6
annotation: SIPC4EE547FFFBA42C1
annotation: SIP191AA3378107D4FB
annotation: SIPED69B315AA534717
annotation: SIPC30B8DCD2C0279B5
annotation: SIP9156FB113C3AE5E6
annotation: SIP1A7CD5F2333E6259
annotation: SIPB5BADCD650DB2C3B
annotation: SIPD026B2486CCF60B5
annotation: SIP35FB0FF4D5BD138F
annotation: SIPED25FD0484190CD4
annotation: SIP29C2DEB2D2EC0FA4
annotation: SIPE24282F727157D30
annotation: SIPC73BF874CC3504C1
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/378
classified with: GO_0018784
classified with: GO_0018778
sequence: O06652-1

O06652-citation-9209038
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
scope: MUTAGENESIS

http://purl.uniprot.org/brenda/3.8.1.10
type: Resource
database: BRENDA
comment: 5085

IPR019714
type: Resource
database: InterPro
comment: 2-haloacid_dehalogenase_DehI

PF10778
type: Resource
database: Pfam
comment: DehI
signature sequence match: Pfam PF10778 match 1

SIP3972A79D22EABEE8
type: Structured Name
full name: 2-haloacid dehalogenase, configuration-inverting
ec name: 3.8.1.10

SIP698768577824A62E
type: Structured Name
full name: DL-2-haloacid dehalogenase

SIP83BDDFF6849C8FC4
type: Structured Name
full name: DL-DEXi

SIPAE7D1156F3EE2DD1
type: Function Annotation
comment: Dehalogenates both (S)- and (R)-2-haloalkanoic acids to the corresponding (R)- and (S)-hydroxyalkanoic acids, respectively, with inversion of configuration at C-2. Acts on 2-haloalkanoic acids whose carbon chain lengths are five or less.

SIPF891322D8A73E1FE
type: Subunit Annotation
comment: Homodimer.

SIPEA6A7672F2E642AA
type: Similarity Annotation
comment: Belongs to the HAD-like hydrolase superfamily. S-2-haloalkanoic acid dehalogenase family.

PRO_0000079172
type: Chain Annotation
comment: 2-haloacid dehalogenase, configuration-inverting
mass: 34239
range: 22289532555964974tt1tt307

22289532555964974tt1
type: Position
type: ExactPosition
position: 1
reference: O06652-1

22289532555964974tt307
type: Position
type: ExactPosition
position: 307
reference: O06652-1

SIP6F80A3EBB7BD250C
type: Mutagenesis Annotation
comment: 60% activity of wild-type.
substitution: F
range: 22289532555964974tt26tt26

22289532555964974tt26
type: Position
type: ExactPosition
position: 26
reference: O06652-1

SIPA9CED1A980B90C0B
type: Mutagenesis Annotation
comment: Less than 10% activity.
substitution: N
range: 22289532555964974tt28tt28

22289532555964974tt28
type: Position
type: ExactPosition
position: 28
reference: O06652-1

SIPBFFD5D4E674E20EF
type: Mutagenesis Annotation
comment: Almost no effect.
substitution: A
range: 22289532555964974tt34tt34

22289532555964974tt34
type: Position
type: ExactPosition
position: 34
reference: O06652-1

SIP3ED21539FB40A723
type: Mutagenesis Annotation
comment: 40% activity of wild-type.
substitution: F
range: 22289532555964974tt37tt37

22289532555964974tt37
type: Position
type: ExactPosition
position: 37
reference: O06652-1

SIPC230AC0A148FB4E6
type: Mutagenesis Annotation
comment: Almost no effect.
substitution: A
range: 22289532555964974tt43tt43

22289532555964974tt43
type: Position
type: ExactPosition
position: 43
reference: O06652-1

SIP8A43AD98A46B52A9
type: Mutagenesis Annotation
comment: Complete loss of activity.
substitution: A
range: 22289532555964974tt65tt65

22289532555964974tt65
type: Position
type: ExactPosition
position: 65
reference: O06652-1

SIP5727C0291424F173
type: Mutagenesis Annotation
comment: Complete loss of activity.
substitution: D
substitution: N
substitution: Q
range: 22289532555964974tt69tt69

22289532555964974tt69
type: Position
type: ExactPosition
position: 69
reference: O06652-1

SIP736002E08D5614A0
type: Mutagenesis Annotation
comment: 50% activity of wild-type.
substitution: N
range: 22289532555964974tt73tt73

22289532555964974tt73
type: Position
type: ExactPosition
position: 73
reference: O06652-1

SIP2FB3AF9162E23F10
type: Mutagenesis Annotation
comment: Almost no effect.
substitution: A
range: 22289532555964974tt76tt76

22289532555964974tt76
type: Position
type: ExactPosition
position: 76
reference: O06652-1

SIP7CFC3C6D7271EB51
type: Mutagenesis Annotation
comment: 15% activity of wild-type.
substitution: Q
range: 22289532555964974tt102tt102

22289532555964974tt102
type: Position
type: ExactPosition
position: 102
reference: O06652-1

SIP32C299D00551310F
type: Mutagenesis Annotation
comment: Almost no effect.
substitution: F
range: 22289532555964974tt115tt115

22289532555964974tt115
type: Position
type: ExactPosition
position: 115
reference: O06652-1

SIPA41AC780AC7FCA78
type: Mutagenesis Annotation
comment: Less than 10% activity.
substitution: D
range: 22289532555964974tt117tt117

22289532555964974tt117
type: Position
type: ExactPosition
position: 117
reference: O06652-1

SIPBF83325BCE1C5AD6
type: Mutagenesis Annotation
comment: Almost no effect.
substitution: R
range: 22289532555964974tt119tt119

22289532555964974tt119
type: Position
type: ExactPosition
position: 119
reference: O06652-1

SIPC4EE547FFFBA42C1
type: Mutagenesis Annotation
comment: Less than 10% activity.
substitution: F
range: 22289532555964974tt120tt120

22289532555964974tt120
type: Position
type: ExactPosition
position: 120
reference: O06652-1

SIP191AA3378107D4FB
type: Mutagenesis Annotation
comment: 60% activity of wild-type.
substitution: A
range: 22289532555964974tt125tt125

22289532555964974tt125
type: Position
type: ExactPosition
position: 125
reference: O06652-1

SIPED69B315AA534717
type: Mutagenesis Annotation
comment: No effect.
substitution: Q
range: 22289532555964974tt129tt129

22289532555964974tt129
type: Position
type: ExactPosition
position: 129
reference: O06652-1

SIPC30B8DCD2C0279B5
type: Mutagenesis Annotation
comment: 50% activity of wild-type.
substitution: A
range: 22289532555964974tt134tt134

22289532555964974tt134
type: Position
type: ExactPosition
position: 134
reference: O06652-1

SIP9156FB113C3AE5E6
type: Mutagenesis Annotation
comment: No effect.
substitution: Q
range: 22289532555964974tt161tt161

22289532555964974tt161
type: Position
type: ExactPosition
position: 161
reference: O06652-1

SIP1A7CD5F2333E6259
type: Mutagenesis Annotation
comment: No effect.
substitution: N
range: 22289532555964974tt167tt167

22289532555964974tt167
type: Position
type: ExactPosition
position: 167
reference: O06652-1

SIPB5BADCD650DB2C3B
type: Mutagenesis Annotation
comment: No effect.
substitution: A
range: 22289532555964974tt193tt193

22289532555964974tt193
type: Position
type: ExactPosition
position: 193
reference: O06652-1

SIPD026B2486CCF60B5
type: Mutagenesis Annotation
comment: Complete loss of activity.
substitution: A
substitution: E
substitution: G
substitution: N
substitution: S
range: 22289532555964974tt194tt194

22289532555964974tt194
type: Position
type: ExactPosition
position: 194
reference: O06652-1

SIP35FB0FF4D5BD138F
type: Mutagenesis Annotation
comment: 60% activity of wild-type.
substitution: F
range: 22289532555964974tt201tt201

22289532555964974tt201
type: Position
type: ExactPosition
position: 201
reference: O06652-1

SIPED25FD0484190CD4
type: Mutagenesis Annotation
comment: Less than 10% activity.
substitution: A
range: 22289532555964974tt219tt219

22289532555964974tt219
type: Position
type: ExactPosition
position: 219
reference: O06652-1

SIP29C2DEB2D2EC0FA4
type: Mutagenesis Annotation
comment: Less than 10% activity.
substitution: N
range: 22289532555964974tt250tt250

22289532555964974tt250
type: Position
type: ExactPosition
position: 250
reference: O06652-1

SIPE24282F727157D30
type: Mutagenesis Annotation
comment: Almost no effect.
substitution: A
range: 22289532555964974tt256tt256

22289532555964974tt256
type: Position
type: ExactPosition
position: 256
reference: O06652-1

SIPC73BF874CC3504C1
type: Mutagenesis Annotation
comment: Almost no effect.
substitution: D
range: 22289532555964974tt271tt271

22289532555964974tt271
type: Position
type: ExactPosition
position: 271
reference: O06652-1

O06652-1
type: Simple Sequence
modified: 1997-07-01
version: 1
mass: 34239
value: MSHRPILKNFPQVDHHQASGKLGDLYNDIHDTLRVPWVAFGIRVMSQFEHFVPAAWEALKPQISTRYAEEGADKVREAAIIPGSAPANPTPALLANGWSEEEIAKLKATLDGLNYGNPKYLILISAWNEAWHGRDAGGGAGKRLDSVQSERLPYGLPQGVEKFHLIDPEAADDQVQCLLRDIRDAFLHHGPASDYRVLAAWPDYLEIAFRDTLKPVALTTEFELTTSRIRKIAREHVRGFDGAGGVAWRDMADRMTPEEIAGLTGVLFMYNRFIADITVAIIRLKQAFGSAEDATENKFRVWPTEKG

http://purl.uniprot.org/uniprot/O06652#attribution-2FC579F523232917B70D579F775AF905
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/9209038

http://purl.uniprot.org/uniprot/O06652#attribution-080ED3982FEC43F7C43A0689717F3D20
manual: true
evidence: ECO_0000305

Q53464
type: Protein
reviewed: true
created: 1998-07-15
modified: 2023-09-13
version: 94
mnemonic: HAD_PSEUY
old mnemonic: HAD_PSESP
citation: http://purl.uniprot.org/citations/7944368
citation: http://purl.uniprot.org/citations/7490277
citation: http://purl.uniprot.org/citations/8702766
citation: http://purl.uniprot.org/citations/9614112
citation: SIPA752891D43F5EA25
recommended name: SIPA7C1300272FED769
alternative name: SIP3D59EBE54E0716E0
alternative name: SIPC64FAB9B98A18FEC
alternative name: SIP849A1E5A8104FAEF
alternative name: SIPD1739D6691864016
enzyme: http://purl.uniprot.org/enzyme/3.8.1.2
organism: http://purl.uniprot.org/taxonomy/66693
annotation: SIP06F66052B12937ED
annotation: SIP61EAB1C569A68CC8
annotation: SIP4DDF55EFEB7D9B27
annotation: SIP10534D330CE49D98
annotation: SIP32EFE82DD171611D
annotation: SIP38B1DBBD719652E9
annotation: SIP68EA00AF95C69373
annotation: PRO_0000079166
annotation: SIPA7C659687E06504B
annotation: SIP034D53D6B5CACA53
annotation: SIP658DE3D2BFCC5829
annotation: SIPD7FD659C087DC9EB
annotation: SIPEA70D04551326E77
annotation: SIP9503DED07FA1F1E4
annotation: SIP91EE13622101AD00
annotation: SIP0502B2F929E6E8CC
annotation: SIP00D6C1FB35FD3EF6
annotation: SIP895C764A65A84066
annotation: SIP94715D37A7EFF6E3
annotation: SIPD4841EAEC0EE7B25
annotation: SIP1EA67E8EEFF9601B
annotation: SIPA4950A95EF40C633
annotation: SIP5C57C615B6CBCC58
annotation: SIP80550AAE758B686A
annotation: SIP3D7AAC8A8AE17F2E
annotation: SIPDD18BE88CE100C1B
annotation: SIPE68D5E44AC6CBE7E
annotation: SIP9EC63D5DA247615A
annotation: SIPA09403BF61C8A244
annotation: SIPDC2145B592B71814
annotation: SIPA2E2B4802AFBE946
annotation: SIPAE670ED31C98A328
annotation: SIPB983A97D9763E123
annotation: SIP084695DDD443D1E6
annotation: SIP72B65A429F2C8C45
annotation: SIP7FF28AEDCC867CE6
annotation: SIP93DF1BE6E6E3EF44
annotation: SIPD8DD23DBC14B8569
annotation: SIP4859401825F721BE
annotation: SIP6154884E1CBFDAF0
annotation: SIP66F244A115170BDC
annotation: SIP5832BE97F0FA66C7
annotation: SIP050FE0FA69CB2441
annotation: SIPA079677AA5644938
annotation: SIPB3ACDECEA500D4EF
annotation: SIP6AD5C32DCE58139F
annotation: SIPA1DA8E78CAAF860B
annotation: SIPB4F739FA4AE7F874
annotation: SIPFD81E5979544CBDF
annotation: SIPF8EF3440E978FC95
annotation: SIPD5246D80CDFC4E44
annotation: SIP1DDC71F5829F6DE4
annotation: SIPD5E08BCB9862E7DB
annotation: SIP0CB15A5E5E4C221B
annotation: SIP5CFA8D2B46B64BEE
annotation: SIP308D66B7703DE65C
annotation: SIPD7ED5B0B42007C3D
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/2
classified with: http://purl.uniprot.org/keywords/378
classified with: GO_0018784

Q53464-citation-7944368
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

Q53464-citation-7490277
type: Citation Statement
scope: FUNCTION
scope: CATALYTIC ACTIVITY
scope: BIOPHYSICOCHEMICAL PROPERTIES
scope: ACTIVE SITE
scope: MUTAGENESIS OF ASP-10; THR-14; ARG-41; SER-118; LYS-151; TYR-157; SER-175; ASN-177 AND ASP-180

Q53464-citation-8702766
type: Citation Statement
scope: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS)
scope: SUBUNIT

Q53464-citation-9614112
type: Citation Statement
scope: X-RAY CRYSTALLOGRAPHY (1.83 ANGSTROMS) OF MUTANT ALA-175 IN COMPLEX WITH MONOCHLOROACETATE AND 2-CHLORO-N-BUTYRATE
scope: FUNCTION
scope: CATALYTIC ACTIVITY
scope: ACTIVE SITE
scope: MUTAGENESIS OF SER-175

SIPA752891D43F5EA25
type: Submission Citation
title: The Structure of L-2-Haloacid Dehalogenase Complexed with a Reaction Product Reveals the Mechanism of Intermediate Hydrolysis in Dehalogenase.
author: Li Y.-F.
author: Hata Y.
author: Fujii T.
author: Kurihara T.
author: Esaki N.
date: 1999-05
submitted to: PDB

Q53464-citation-SIPA752891D43F5EA25
type: Citation Statement
scope: X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS) IN COMPLEX WITH LACTATE

1JUD
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.50
chain sequence mapping: PDB 1JUD tt1tt232

PDB 1JUD tt1tt232
chain: A=1-232

1QH9
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.50
chain sequence mapping: PDB 1QH9 tt1tt232

PDB 1QH9 tt1tt232
chain: A=1-232

1ZRM
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.00
chain sequence mapping: PDB 1ZRM tt1tt232

PDB 1ZRM tt1tt232
chain: A=1-232

1ZRN
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 1.83
chain sequence mapping: PDB 1ZRN tt1tt232

PDB 1ZRN tt1tt232
chain: A=1-232

DB03568
type: Resource
database: DrugBank
comment: Butyric Acid

DB03066
type: Resource
database: DrugBank
comment: D-Lactic acid

SIPA7C1300272FED769
type: Structured Name
full name: (S)-2-haloacid dehalogenase
ec name: 3.8.1.2

SIP3D59EBE54E0716E0
type: Structured Name
full name: 2-haloalkanoic acid dehalogenase

SIPC64FAB9B98A18FEC
type: Structured Name
full name: Halocarboxylic acid halidohydrolase

SIP849A1E5A8104FAEF
type: Structured Name
full name: L-2-haloacid dehalogenase

SIPD1739D6691864016
type: Structured Name
full name: L-DEX

SIP06F66052B12937ED
type: Function Annotation
comment: Catalyzes the hydrolytic dehalogenation of small (S)-2-haloalkanoic acids to yield the corresponding (R)-2-hydroxyalkanoic acids (PubMed:7490277, PubMed:9614112). Acts on acids of short chain lengths, C(2) to C(4), with inversion of configuration at C-2 (PubMed:7490277, PubMed:9614112). Active with 2-halogenated carboxylic acids and converts only the S-isomer (or L-isomer) of 2-chloropropionic acid with inversion of configuration to produce R-lactate (or D-isomer) (PubMed:7490277, PubMed:9614112).

SIP10534D330CE49D98
type: Kinetics Annotation
comment: kcat is 47 sec(-1) for (2S)-2-chloropropanoic acid as substrate.
measured affinity: 0.37 mM for (2S)-2-chloropropanoic acid

SIP32EFE82DD171611D
type: Subunit Annotation
comment: Homodimer.

SIP38B1DBBD719652E9
type: Biotechnology Annotation
comment: (S)-2-haloacid dehalogenases may be used for the biodegradation of halogenated substances and their derivatives which are widely used as pesticides, herbicides and other industrial products.

SIP68EA00AF95C69373
type: Similarity Annotation
comment: Belongs to the HAD-like hydrolase superfamily. S-2-haloalkanoic acid dehalogenase family.

PRO_0000079166
type: Chain Annotation
comment: (S)-2-haloacid dehalogenase
mass: 26177
range: 22857967149134894tt1tt232

22857967149134894tt1
type: Position
type: ExactPosition
position: 1
reference: Q53464-1

22857967149134894tt232
type: Position
type: ExactPosition
position: 232
reference: Q53464-1

SIPA7C659687E06504B
type: Region Annotation
comment: Important for catalytic activity
range: 22857967149134894tt175tt180

22857967149134894tt175
type: Position
type: ExactPosition
position: 175
reference: Q53464-1

22857967149134894tt180
type: Position
type: ExactPosition
position: 180
reference: Q53464-1

SIP034D53D6B5CACA53
type: Active Site Annotation
comment: Nucleophile
range: 22857967149134894tt10tt10

22857967149134894tt10
type: Position
type: ExactPosition
position: 10
reference: Q53464-1

22857967149134894tt11
type: Position
type: ExactPosition
position: 11
reference: Q53464-1

22857967149134894tt12
type: Position
type: ExactPosition
position: 12
reference: Q53464-1

22857967149134894tt41
type: Position
type: ExactPosition
position: 41
reference: Q53464-1

22857967149134894tt118
type: Position
type: ExactPosition
position: 118
reference: Q53464-1

22857967149134894tt119
type: Position
type: ExactPosition
position: 119
reference: Q53464-1

SIP9503DED07FA1F1E4
type: Site Annotation
comment: Important for catalytic activity
range: 22857967149134894tt14tt14

22857967149134894tt14
type: Position
type: ExactPosition
position: 14
reference: Q53464-1

SIP91EE13622101AD00
type: Site Annotation
comment: Important for catalytic activity
range: 22857967149134894tt151tt151

22857967149134894tt151
type: Position
type: ExactPosition
position: 151
reference: Q53464-1

SIP0502B2F929E6E8CC
type: Site Annotation
comment: Important for catalytic activity
range: 22857967149134894tt157tt157

22857967149134894tt157
type: Position
type: ExactPosition
position: 157
reference: Q53464-1

SIP00D6C1FB35FD3EF6
type: Mutagenesis Annotation
comment: Loss of catalytic activity.
substitution: E
substitution: S
substitution: A
substitution: G
range: 22857967149134894tt10tt10

SIP895C764A65A84066
type: Mutagenesis Annotation
comment: Severe reduction in catalytic activity.
substitution: N
range: 22857967149134894tt10tt10

SIP94715D37A7EFF6E3
type: Mutagenesis Annotation
comment: Severe reduction in catalytic activity. No effect on susbtrate affinity.
substitution: A
range: 22857967149134894tt14tt14

SIPD4841EAEC0EE7B25
type: Mutagenesis Annotation
comment: Severe reduction in catalytic activity.
substitution: P
substitution: C
range: 22857967149134894tt14tt14

SIP1EA67E8EEFF9601B
type: Mutagenesis Annotation
comment: Mild reduction in catalytic activity.
substitution: S
range: 22857967149134894tt14tt14

SIPA4950A95EF40C633
type: Mutagenesis Annotation
comment: Severe reduction in catalytic activity.
substitution: E
range: 22857967149134894tt41tt41

SIP5C57C615B6CBCC58
type: Mutagenesis Annotation
comment: Severe reduction in catalytic activity. 4-fold decrease in substrate affinity.
substitution: K
range: 22857967149134894tt41tt41

SIP80550AAE758B686A
type: Mutagenesis Annotation
comment: Loss of catalytic activity.
substitution: W
range: 22857967149134894tt41tt41

SIP3D7AAC8A8AE17F2E
type: Mutagenesis Annotation
comment: Severe reduction in catalytic activity. 25-fold decrease in substrate affinity.
substitution: A
range: 22857967149134894tt118tt118

SIPDD18BE88CE100C1B
type: Mutagenesis Annotation
comment: Loss of catalytic activity.
substitution: A
range: 22857967149134894tt151tt151

SIPE68D5E44AC6CBE7E
type: Mutagenesis Annotation
comment: Severe reduction in catalytic activity.
substitution: R
range: 22857967149134894tt151tt151

SIP9EC63D5DA247615A
type: Mutagenesis Annotation
comment: Severe reduction in catalytic activity.
substitution: C
substitution: S
substitution: H
substitution: W
range: 22857967149134894tt157tt157

SIPA09403BF61C8A244
type: Mutagenesis Annotation
comment: Severe reduction in catalytic activity. 1.5-fold decrease in substrate affinity.
substitution: F
range: 22857967149134894tt157tt157

SIPDC2145B592B71814
type: Mutagenesis Annotation
comment: Severe reduction in catalytic activity. 2-fold decrease in substrate affinity.
substitution: A
range: 22857967149134894tt175tt175

SIPA2E2B4802AFBE946
type: Mutagenesis Annotation
comment: Severe reduction in catalytic activity.
substitution: C
range: 22857967149134894tt175tt175

SIPAE670ED31C98A328
type: Mutagenesis Annotation
comment: Moderate reduction in catalytic activity.
substitution: T
range: 22857967149134894tt175tt175

SIPB983A97D9763E123
type: Mutagenesis Annotation
comment: Loss of catalytic activity.
substitution: D
substitution: I
range: 22857967149134894tt177tt177

22857967149134894tt177
type: Position
type: ExactPosition
position: 177
reference: Q53464-1

SIP084695DDD443D1E6
type: Mutagenesis Annotation
comment: Severe reduction in catalytic activity.
substitution: W
substitution: K
substitution: H
substitution: Q
range: 22857967149134894tt177tt177

SIP72B65A429F2C8C45
type: Mutagenesis Annotation
comment: Loss of catalytic activity.
substitution: N
substitution: E
substitution: S
substitution: G
range: 22857967149134894tt180tt180

22857967149134894tt6
type: Position
type: ExactPosition
position: 6
reference: Q53464-1

22857967149134894tt9
type: Position
type: ExactPosition
position: 9
reference: Q53464-1

22857967149134894tt13
type: Position
type: ExactPosition
position: 13
reference: Q53464-1

22857967149134894tt15
type: Position
type: ExactPosition
position: 15
reference: Q53464-1

22857967149134894tt19
type: Position
type: ExactPosition
position: 19
reference: Q53464-1

22857967149134894tt28
type: Position
type: ExactPosition
position: 28
reference: Q53464-1

22857967149134894tt30
type: Position
type: ExactPosition
position: 30
reference: Q53464-1

22857967149134894tt32
type: Position
type: ExactPosition
position: 32
reference: Q53464-1

22857967149134894tt33
type: Position
type: ExactPosition
position: 33
reference: Q53464-1

22857967149134894tt54
type: Position
type: ExactPosition
position: 54
reference: Q53464-1

22857967149134894tt60
type: Position
type: ExactPosition
position: 60
reference: Q53464-1

22857967149134894tt75
type: Position
type: ExactPosition
position: 75
reference: Q53464-1

22857967149134894tt81
type: Position
type: ExactPosition
position: 81
reference: Q53464-1

22857967149134894tt89
type: Position
type: ExactPosition
position: 89
reference: Q53464-1

22857967149134894tt90
type: Position
type: ExactPosition
position: 90
reference: Q53464-1

22857967149134894tt93
type: Position
type: ExactPosition
position: 93
reference: Q53464-1

22857967149134894tt100
type: Position
type: ExactPosition
position: 100
reference: Q53464-1

22857967149134894tt109
type: Position
type: ExactPosition
position: 109
reference: Q53464-1

22857967149134894tt113
type: Position
type: ExactPosition
position: 113
reference: Q53464-1

22857967149134894tt120
type: Position
type: ExactPosition
position: 120
reference: Q53464-1

22857967149134894tt122
type: Position
type: ExactPosition
position: 122
reference: Q53464-1

22857967149134894tt131
type: Position
type: ExactPosition
position: 131
reference: Q53464-1

22857967149134894tt135
type: Position
type: ExactPosition
position: 135
reference: Q53464-1

22857967149134894tt137
type: Position
type: ExactPosition
position: 137
reference: Q53464-1

22857967149134894tt139
type: Position
type: ExactPosition
position: 139
reference: Q53464-1

22857967149134894tt144
type: Position
type: ExactPosition
position: 144
reference: Q53464-1

22857967149134894tt145
type: Position
type: ExactPosition
position: 145
reference: Q53464-1

22857967149134894tt147
type: Position
type: ExactPosition
position: 147
reference: Q53464-1

22857967149134894tt154
type: Position
type: ExactPosition
position: 154
reference: Q53464-1

22857967149134894tt164
type: Position
type: ExactPosition
position: 164
reference: Q53464-1

22857967149134894tt168
type: Position
type: ExactPosition
position: 168
reference: Q53464-1

22857967149134894tt170
type: Position
type: ExactPosition
position: 170
reference: Q53464-1

22857967149134894tt171
type: Position
type: ExactPosition
position: 171
reference: Q53464-1

22857967149134894tt176
type: Position
type: ExactPosition
position: 176
reference: Q53464-1

22857967149134894tt178
type: Position
type: ExactPosition
position: 178
reference: Q53464-1

22857967149134894tt187
type: Position
type: ExactPosition
position: 187
reference: Q53464-1

22857967149134894tt191
type: Position
type: ExactPosition
position: 191
reference: Q53464-1

22857967149134894tt194
type: Position
type: ExactPosition
position: 194
reference: Q53464-1

22857967149134894tt203
type: Position
type: ExactPosition
position: 203
reference: Q53464-1

22857967149134894tt205
type: Position
type: ExactPosition
position: 205
reference: Q53464-1

22857967149134894tt209
type: Position
type: ExactPosition
position: 209
reference: Q53464-1

22857967149134894tt214
type: Position
type: ExactPosition
position: 214
reference: Q53464-1

22857967149134894tt215
type: Position
type: ExactPosition
position: 215
reference: Q53464-1

22857967149134894tt219
type: Position
type: ExactPosition
position: 219
reference: Q53464-1

Q53464-1
type: Simple Sequence
modified: 1996-11-01
version: 1
mass: 26177
value: MDYIKGIAFDLYGTLFDVHSVVGRCDEAFPGRGREISALWRQKQLEYTWLRSLMNRYVNFQQATEDALRFTCRHLGLDLDARTRSTLCDAYLRLAPFSEVPDSLRELKRRGLKLAILSNGSPQSIDAVVSHAGLRDGFDHLLSVDPVQVYKPDNRVYELAEQALGLDRSAILFVSSNAWDATGARYFGFPTCWINRTGNVFEEMGQTPDWEVTSLRAVVELFETAAGKAEKG

http://purl.uniprot.org/uniprot/Q53464#attribution-F43472C01D8891AFA23DEE5B8AA313C5
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/7490277

http://purl.uniprot.org/uniprot/Q53464#attribution-6BEBECC8502EB4DD75735B8FCB9358B3
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/8702766

http://purl.uniprot.org/uniprot/Q53464#attribution-6D8E5EF2FFB069DF7A665612266C8D92
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/9614112

http://purl.uniprot.org/uniprot/Q53464#attribution-CF2E3BB912F48C9A25CE08FADFEDAD26
manual: true
evidence: ECO_0000269
source: SIPA752891D43F5EA25

http://purl.uniprot.org/uniprot/Q53464#attribution-AE805E10D56A43E09589B5D3B178A7CB
manual: true
evidence: ECO_0000303
source: http://purl.uniprot.org/citations/7944368

http://purl.uniprot.org/uniprot/Q53464#attribution-F056FFA9203390C71E154C607B49960E
manual: true
evidence: ECO_0000305

http://purl.uniprot.org/uniprot/Q53464#attribution-E6E9FBE96386B5916FCADE64A60AE55F
manual: true
evidence: ECO_0000305
source: http://purl.uniprot.org/citations/7490277

http://purl.uniprot.org/uniprot/Q53464#attribution-259B847E1BD2E43E21B22D5834424628
manual: true
evidence: ECO_0000305
source: http://purl.uniprot.org/citations/9614112

http://purl.uniprot.org/uniprot/Q53464#attribution-BAE9548DB71F2041DB7B438CAA623184
manual: true
evidence: ECO_0007744
source: 1JUD

http://purl.uniprot.org/uniprot/Q53464#attribution-AC17CEEBC9F2253A62A9DEA397F2BCA7
manual: true
evidence: ECO_0007744
source: 1QH9

http://purl.uniprot.org/uniprot/Q53464#attribution-248530AD2D3612C25624080D9DDB944A
manual: true
evidence: ECO_0007744
source: 1ZRM

http://purl.uniprot.org/uniprot/Q53464#attribution-14A050A989919348BBC56B7626F83D35
manual: true
evidence: ECO_0007744
source: 1ZRN

Q8KY27
type: Protein
reviewed: true
created: 2003-05-16
modified: 2023-05-03
version: 61
mnemonic: HIS7_AMIAM
citation: SIPE501D1D44EB223C4
recommended name: SIPCACD72F38863E391
enzyme: http://purl.uniprot.org/enzyme/4.2.1.19
organism: http://purl.uniprot.org/taxonomy/135579
annotation: SIP4AF44BB5D969B2F3
annotation: SIP757D9B45302B429A
annotation: SIP6DA774902929C994
annotation: SIP30499EC72F8D204B
annotation: PRO_0000158098
existence: Inferred from Homology Existence
classified with: http://purl.uniprot.org/keywords/963
classified with: http://purl.uniprot.org/keywords/368
classified with: http://purl.uniprot.org/keywords/456
classified with: GO_0005737
classified with: GO_0004424
classified with: GO_0000105
sequence: MRNAEVSRNTLETKIAVAINLDGTGVSKLNSGVGFFDHMLDQIARHGMMDISVECQGDLHIDAHHTVEDVGIALGQAFSRALGDKKGIRRYAHAYVPLDEALSRVVLDISGRPGLEFNVEFTRARIGEFDVDLVREFFQGFVNHAALTLHVDNLRGKNAHHQAETIFKAFGRALRAAVELDPRMVGIMPSTKGSL

SIPE501D1D44EB223C4
type: Submission Citation
title: The 3-hexulose-6-phosphate synthase-encoding gene of the obligate methylotroph Aminomonas aminovorus C2A1 is not present on a gene cluster encoding other enzymes of the ribulose monophosphate pathway.
author: Taylor E.J.
author: Smith N.L.
author: Colby J.
author: Black G.W.
date: 2002-07
submitted to: EMBL/GenBank/DDBJ

Q8KY27-citation-SIPE501D1D44EB223C4
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

DSM 15625 / NCIMB 11268 / C2A1
type: Strain

cd07914
type: Resource
database: CDD
comment: IGPD
signature sequence match: CDD cd07914 match 1

MF_00076
type: Resource
database: HAMAP
comment: HisB
signature sequence match: HAMAP MF 00076 match 1

IPR038494
type: Resource
database: InterPro
comment: IGPD_sf

IPR000807
type: Resource
database: InterPro
comment: ImidazoleglycerolP_deHydtase

IPR020565
type: Resource
database: InterPro
comment: ImidazoleglycerP_deHydtase_CS

IPR020568
type: Resource
database: InterPro
comment: Ribosomal_S5_D2-typ_fold

PTHR23133:SF2
type: Resource
database: PANTHER
comment: IMIDAZOLEGLYCEROL-PHOSPHATE DEHYDRATASE
signature sequence match: PANTHER PTHR23133:SF2 match 1

PTHR23133
type: Resource
database: PANTHER
comment: IMIDAZOLEGLYCEROL-PHOSPHATE DEHYDRATASE HIS7
signature sequence match: PANTHER PTHR23133 match 1

PF00475
type: Resource
database: Pfam
comment: IGPD
signature sequence match: Pfam PF00475 match 1

SSF54211
type: Resource
database: SUPFAM
comment: Ribosomal protein S5 domain 2-like
signature sequence match: SUPFAM SSF54211 match 1
signature sequence match: SUPFAM SSF54211 match 2

PS00954
type: Resource
database: PROSITE
comment: IGP_DEHYDRATASE_1
signature sequence match: PROSITE PS00954 match 1

PS00955
type: Resource
database: PROSITE
comment: IGP_DEHYDRATASE_2
signature sequence match: PROSITE PS00955 match 1

SIPCACD72F38863E391
type: Structured Name
full name: Imidazoleglycerol-phosphate dehydratase
short name: IGPD
ec name: 4.2.1.19

hisB
type: Gene

SIP30499EC72F8D204B
type: Similarity Annotation
comment: Belongs to the imidazoleglycerol-phosphate dehydratase family.

PRO_0000158098
type: Chain Annotation
comment: Imidazoleglycerol-phosphate dehydratase
mass: 21446
range: 22861369383728942tt1tt195

22861369383728942tt1
type: Position
type: ExactPosition
position: 1
reference: MRNAEVSRNTLETKIAVAINLDGTGVSKLNSGVGFFDHMLDQIARHGMMDISVECQGDLHIDAHHTVEDVGIALGQAFSRALGDKKGIRRYAHAYVPLDEALSRVVLDISGRPGLEFNVEFTRARIGEFDVDLVREFFQGFVNHAALTLHVDNLRGKNAHHQAETIFKAFGRALRAAVELDPRMVGIMPSTKGSL

22861369383728942tt195
type: Position
type: ExactPosition
position: 195
reference: MRNAEVSRNTLETKIAVAINLDGTGVSKLNSGVGFFDHMLDQIARHGMMDISVECQGDLHIDAHHTVEDVGIALGQAFSRALGDKKGIRRYAHAYVPLDEALSRVVLDISGRPGLEFNVEFTRARIGEFDVDLVREFFQGFVNHAALTLHVDNLRGKNAHHQAETIFKAFGRALRAAVELDPRMVGIMPSTKGSL

MRNAEVSRNTLETKIAVAINLDGTGVSKLNSGVGFFDHMLDQIARHGMMDISVECQGDLHIDAHHTVEDVGIALGQAFSRALGDKKGIRRYAHAYVPLDEALSRVVLDISGRPGLEFNVEFTRARIGEFDVDLVREFFQGFVNHAALTLHVDNLRGKNAHHQAETIFKAFGRALRAAVELDPRMVGIMPSTKGSL
type: Simple Sequence
modified: 2002-10-01
version: 1
mass: 21446

http://purl.uniprot.org/uniprot/Q8KY27#attribution-31CA1A09386620FE1D44FDA80E3806B5
manual: true
evidence: ECO_0000255
source: MF_00076

P51014
type: Protein
reviewed: true
created: 1996-10-01
modified: 2023-09-13
version: 69
mnemonic: HOA_PSES1
old mnemonic: BPHF_PSES1
citation: http://purl.uniprot.org/citations/8021212
recommended name: SIP5C0705BDF7A69FF4
alternative name: SIPCD6CB1FEDBEC70E8
alternative name: SIP35C27E77B78D28BB
enzyme: http://purl.uniprot.org/enzyme/4.1.3.39
organism: http://purl.uniprot.org/taxonomy/307
annotation: SIP37ED35A43DF18FEB
annotation: SIPAE9B6EFA66A1489A
annotation: SIPAABC41350744C17A
annotation: PRO_0000064977
annotation: SIP30B4EE99447F604E
annotation: SIP138142722C00C8DD
annotation: SIPED9B4E5BA0C89181
annotation: SIP449C14F7CB8A8064
annotation: SIP5C5B58466E7036BD
annotation: SIP2E2881148DECBFF1
annotation: SIP54E9957BF543C85C
annotation: SIP386877924D38F4D4
annotation: SIPFB2B49887E5FE034
annotation: SIPE38EA927C4973F42
existence: Inferred from Homology Existence
classified with: http://purl.uniprot.org/keywords/58
classified with: http://purl.uniprot.org/keywords/456
classified with: http://purl.uniprot.org/keywords/464
classified with: http://purl.uniprot.org/keywords/479
classified with: GO_0008701
classified with: GO_0030145
classified with: GO_0019439
sequence: P51014-1

P51014-citation-8021212
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

cd07943
type: Resource
database: CDD
comment: DRE_TIM_HOA
signature sequence match: CDD cd07943 match 1

http://purl.uniprot.org/gene3d/3.20.20.70
type: Resource
database: Gene3D
comment: Aldolase class I
signature sequence match: Gene3D 3.20.20.70 match 1

MF_01656
type: Resource
database: HAMAP
comment: HOA
signature sequence match: HAMAP MF 01656 match 1

IPR017629
type: Resource
database: InterPro
comment: 4OH_2_O-val_aldolase

IPR013785
type: Resource
database: InterPro
comment: Aldolase_TIM

IPR012425
type: Resource
database: InterPro
comment: DmpG_comm

IPR035685
type: Resource
database: InterPro
comment: DRE_TIM_HOA

IPR000891
type: Resource
database: InterPro
comment: PYR_CT

TIGR03217
type: Resource
database: NCBIfam
comment: 4OH_2_O_val_ald
signature sequence match: NCBIfam TIGR03217 match 1

PTHR10277:SF9
type: Resource
database: PANTHER
comment: 2-ISOPROPYLMALATE SYNTHASE 1, CHLOROPLASTIC-RELATED
signature sequence match: PANTHER PTHR10277:SF9 match 1

PTHR10277
type: Resource
database: PANTHER
comment: HOMOCITRATE SYNTHASE-RELATED
signature sequence match: PANTHER PTHR10277 match 1

PF07836
type: Resource
database: Pfam
comment: DmpG_comm
signature sequence match: Pfam PF07836 match 1

PF00682
type: Resource
database: Pfam
comment: HMGL-like
signature sequence match: Pfam PF00682 match 1

SSF51569
type: Resource
database: SUPFAM
comment: Aldolase
signature sequence match: SUPFAM SSF51569 match 1

SSF89000
type: Resource
database: SUPFAM
comment: post-HMGL domain-like
signature sequence match: SUPFAM SSF89000 match 1

PS50991
type: Resource
database: PROSITE
comment: PYR_CT
signature sequence match: PROSITE PS50991 match 1

SIP5C0705BDF7A69FF4
type: Structured Name
full name: 4-hydroxy-2-oxovalerate aldolase
short name: HOA
ec name: 4.1.3.39

SIPCD6CB1FEDBEC70E8
type: Structured Name
full name: 4-hydroxy-2-keto-pentanoic acid aldolase

SIP35C27E77B78D28BB
type: Structured Name
full name: 4-hydroxy-2-oxopentanoate aldolase

bphF
type: Gene

SIPAABC41350744C17A
type: Similarity Annotation
comment: Belongs to the 4-hydroxy-2-oxovalerate aldolase family.

PRO_0000064977
type: Chain Annotation
comment: 4-hydroxy-2-oxovalerate aldolase
mass: 38264
range: 22576483515053102tt1tt352

22576483515053102tt1
type: Position
type: ExactPosition
position: 1
reference: P51014-1

22576483515053102tt352
type: Position
type: ExactPosition
position: 352
reference: P51014-1

SIP30B4EE99447F604E
type: Domain Extent Annotation
comment: Pyruvate carboxyltransferase
range: 22576483515053102tt6tt258

22576483515053102tt6
type: Position
type: ExactPosition
position: 6
reference: P51014-1

22576483515053102tt258
type: Position
type: ExactPosition
position: 258
reference: P51014-1

SIP138142722C00C8DD
type: Active Site Annotation
comment: Proton acceptor
range: 22576483515053102tt18tt18

22576483515053102tt18
type: Position
type: ExactPosition
position: 18
reference: P51014-1

22576483515053102tt14
type: Position
type: ExactPosition
position: 14
reference: P51014-1

22576483515053102tt15
type: Position
type: ExactPosition
position: 15
reference: P51014-1

22576483515053102tt168
type: Position
type: ExactPosition
position: 168
reference: P51014-1

22576483515053102tt197
type: Position
type: ExactPosition
position: 197
reference: P51014-1

22576483515053102tt199
type: Position
type: ExactPosition
position: 199
reference: P51014-1

22576483515053102tt288
type: Position
type: ExactPosition
position: 288
reference: P51014-1

SIPE38EA927C4973F42
type: Site Annotation
comment: Transition state stabilizer
range: 22576483515053102tt14tt14

substrate

P51014-1
type: Simple Sequence
modified: 1996-10-01
version: 1
mass: 38264
value: MTTKKIYISDVTLRDGSHAIRHQYSVEQVRTIAKELDEAKVDSIEVAHGSGLQGSSFNYGFGAHTDLEWIEAVASVVKHAKIATLLLPGIGTIHDLKAAYDAGARIVRVATHCTEADISKQHIEYARHLGMEAVGFLMMSHMSTPQGLAQQAKLMESYGATCCYVVDSGGALSMDDVRMRFRAFKDVLKPETETGIHAHHNLSLGVANSIVAIEEGCDRVDASLAGMGAGAGNAPLEVFIAAAERMGWHHGTELYKLMDAADDIVRPLQDRPVRVDRETLALGYAGVYSSFLRHSEAAAQKYGLKTMDILVELGRRRMVGGQEDMIVDVALDLLKSLEHERIHAQPVSSEAG

http://purl.uniprot.org/uniprot/P51014#attribution-0FFC91602080964BBF1B0641543A1A0F
manual: true
evidence: ECO_0000255
source: MF_01656

P51016
type: Protein
reviewed: true
created: 1996-10-01
modified: 2023-09-13
version: 96
mnemonic: HOA_PSEUF
old mnemonic: DMPG_PSEPU
old mnemonic: DMPG_PSESP
old mnemonic: DMPG_PSEUF
citation: http://purl.uniprot.org/citations/1732207
citation: http://purl.uniprot.org/citations/8419288
citation: http://purl.uniprot.org/citations/12764229
recommended name: SIP5C0705BDF7A69FF4
alternative name: SIPCD6CB1FEDBEC70E8
alternative name: SIP35C27E77B78D28BB
enzyme: http://purl.uniprot.org/enzyme/4.1.3.39
organism: http://purl.uniprot.org/taxonomy/79676
encoded in: pVI150
annotation: SIPF5554B53EF434DC6
annotation: SIP9F231F744F8FAF86
annotation: SIP15BBC8EEBE096285
annotation: SIP453F13B44FCC29D5
annotation: SIPE589747C2824865E
annotation: SIP4997712187F285E4
annotation: SIPE8DD32EAF723AF59
annotation: SIP81890D1FC216C38A
annotation: SIP4FA5DF82F68BA423
annotation: PRO_0000079938
annotation: SIP71AFDB77CE14A961
annotation: SIP2008C5480BFF2D11
annotation: SIPFBCA09B8D01E69DB
annotation: SIP1152BD2B62FA2457
annotation: SIPD3FA13A0E479DCD3
annotation: SIP2A51CAEE039DCAE4
annotation: SIP0FF6819C3C6828B0
annotation: SIP3799E9BFFD57D562
annotation: SIPE59F62A6625868C6
annotation: SIP0BEFFE3DC18706A1
annotation: SIPEAF5B0F7DFB5EA4A
annotation: SIP9939221800500C47
annotation: SIPFD49B34142A9C82B
annotation: SIPF06159FE64838133
annotation: SIP4CAC606791066461
annotation: SIPA596C0E733D914B4
annotation: SIP088A7A7AFA9186EB
annotation: SIP4FB4526393E91447
annotation: SIP37714DA8957F3AAA
annotation: SIP4C1D488943E0DFDE
annotation: SIP803C33298ED135BC
annotation: SIP6B3F4229481169C2
annotation: SIP9AECE20F616ADA2B
annotation: SIP8F9588B7F3D6E956
annotation: SIP8ADCE5535328430A
annotation: SIPE1A2B7C036417837
annotation: SIP8860C139057B1315
annotation: SIP04C38934D905996E
annotation: SIP0D4168BE69F83111
annotation: SIP2C5C69BB3AA18D3B
annotation: SIP62CDA7CC69BFD334
annotation: SIPCF9053B3B32A8C20
annotation: SIPAAF1D181AF0230C1
annotation: SIP6120B4D2F5D01933
annotation: SIP26308C2EB1777745
annotation: SIPC2193AB1B06F577A
annotation: SIP34FF4157AFA985A4
annotation: SIP20A2CC6E74B53749
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/2
classified with: http://purl.uniprot.org/keywords/58
classified with: http://purl.uniprot.org/keywords/903
classified with: http://purl.uniprot.org/keywords/456
classified with: http://purl.uniprot.org/keywords/464
classified with: http://purl.uniprot.org/keywords/479
classified with: http://purl.uniprot.org/keywords/614
classified with: GO_0008701
classified with: GO_0030145
classified with: GO_0019439
classified with: GO_0043640
classified with: GO_0019336
sequence: P51016-1

P51016-citation-1732207
type: Citation Statement
scope: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
scope: FUNCTION

CF600
type: Strain

P51016-citation-8419288
type: Citation Statement
scope: PROTEIN SEQUENCE OF 2-7
scope: CATALYTIC ACTIVITY
scope: SUBSTRATE SPECIFICITY
scope: ACTIVITY REGULATION
scope: SUBUNIT
scope: INTERACTION WITH DMPF
scope: PH DEPENDENCE

P51016-citation-12764229
type: Citation Statement
scope: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) IN COMPLEX WITH DMPF; NAD; MANGANESE AND OXALATE
scope: SUBUNIT
scope: REACTION MECHANISM

PDB 1NVM tt1tt345
chain: A/C/E/G=1-345

P51016
type: Resource
database: IntAct
comment: 1

http://purl.uniprot.org/brenda/4.1.3.39
type: Resource
database: BRENDA
comment: 5085

SIP5C0705BDF7A69FF4
type: Structured Name
full name: 4-hydroxy-2-oxovalerate aldolase
short name: HOA
ec name: 4.1.3.39

SIPCD6CB1FEDBEC70E8
type: Structured Name
full name: 4-hydroxy-2-keto-pentanoic acid aldolase

SIP35C27E77B78D28BB
type: Structured Name
full name: 4-hydroxy-2-oxopentanoate aldolase

pVI150
type: Plasmid

dmpG
type: Gene

SIPF5554B53EF434DC6
type: Function Annotation
comment: Catalyzes the retro-aldol cleavage of 4-hydroxy-2-oxopentanoate to pyruvate and acetaldehyde. Is involved in the meta-cleavage pathway for the degradation of aromatic compounds such as phenols, cresols and catechols.

SIP15BBC8EEBE096285
type: Activity Regulation Annotation
comment: Six- to eight-fold activated by Mn(2+). Retains residual activity after EDTA treatment in vitro. Also activated by NADH and, to a lesser extent, by NAD(+). Strongly inhibited by Zn(2+). Mg(2+) and Ca(2+) have no effect on enzymatic activity.

SIP453F13B44FCC29D5
type: PH Dependence Annotation
comment: Optimum pH is 8.5-9.0.

SIPE8DD32EAF723AF59
type: Subunit Annotation
comment: Heterotetramer composed of two DmpG (aldolase) and two DmpF (dehydrogenase) subunits, which allows a direct channeling of acetaldehyde between the two active sites.

SIP81890D1FC216C38A
type: Similarity Annotation
comment: Belongs to the 4-hydroxy-2-oxovalerate aldolase family.

SIP4FA5DF82F68BA423
type: Initiator Methionine Annotation
comment: Removed
range: 22576483515053614tt1tt1

22576483515053614tt1
type: Position
type: ExactPosition
position: 1
reference: P51016-1

PRO_0000079938
type: Chain Annotation
comment: 4-hydroxy-2-oxovalerate aldolase
mass: 37339
range: 22576483515053614tt2tt345

22576483515053614tt2
type: Position
type: ExactPosition
position: 2
reference: P51016-1

22576483515053614tt345
type: Position
type: ExactPosition
position: 345
reference: P51016-1

SIP71AFDB77CE14A961
type: Domain Extent Annotation
comment: Pyruvate carboxyltransferase
range: 22576483515053614tt9tt261

22576483515053614tt9
type: Position
type: ExactPosition
position: 9
reference: P51016-1

22576483515053614tt261
type: Position
type: ExactPosition
position: 261
reference: P51016-1

SIP2008C5480BFF2D11
type: Active Site Annotation
comment: Proton acceptor
range: 22576483515053614tt21tt21

22576483515053614tt21
type: Position
type: ExactPosition
position: 21
reference: P51016-1

22576483515053614tt17
type: Position
type: ExactPosition
position: 17
reference: P51016-1

22576483515053614tt18
type: Position
type: ExactPosition
position: 18
reference: P51016-1

22576483515053614tt171
type: Position
type: ExactPosition
position: 171
reference: P51016-1

22576483515053614tt200
type: Position
type: ExactPosition
position: 200
reference: P51016-1

22576483515053614tt202
type: Position
type: ExactPosition
position: 202
reference: P51016-1

22576483515053614tt291
type: Position
type: ExactPosition
position: 291
reference: P51016-1

SIP0BEFFE3DC18706A1
type: Site Annotation
comment: Transition state stabilizer
range: 22576483515053614tt17tt17

22576483515053614tt10
type: Position
type: ExactPosition
position: 10
reference: P51016-1

22576483515053614tt13
type: Position
type: ExactPosition
position: 13
reference: P51016-1

22576483515053614tt15
type: Position
type: ExactPosition
position: 15
reference: P51016-1

22576483515053614tt22
type: Position
type: ExactPosition
position: 22
reference: P51016-1

22576483515053614tt23
type: Position
type: ExactPosition
position: 23
reference: P51016-1

22576483515053614tt25
type: Position
type: ExactPosition
position: 25
reference: P51016-1

22576483515053614tt29
type: Position
type: ExactPosition
position: 29
reference: P51016-1

22576483515053614tt42
type: Position
type: ExactPosition
position: 42
reference: P51016-1

22576483515053614tt45
type: Position
type: ExactPosition
position: 45
reference: P51016-1

22576483515053614tt48
type: Position
type: ExactPosition
position: 48
reference: P51016-1

22576483515053614tt60
type: Position
type: ExactPosition
position: 60
reference: P51016-1

22576483515053614tt62
type: Position
type: ExactPosition
position: 62
reference: P51016-1

22576483515053614tt69
type: Position
type: ExactPosition
position: 69
reference: P51016-1

22576483515053614tt77
type: Position
type: ExactPosition
position: 77
reference: P51016-1

22576483515053614tt81
type: Position
type: ExactPosition
position: 81
reference: P51016-1

22576483515053614tt89
type: Position
type: ExactPosition
position: 89
reference: P51016-1

22576483515053614tt96
type: Position
type: ExactPosition
position: 96
reference: P51016-1

22576483515053614tt105
type: Position
type: ExactPosition
position: 105
reference: P51016-1

22576483515053614tt109
type: Position
type: ExactPosition
position: 109
reference: P51016-1

22576483515053614tt115
type: Position
type: ExactPosition
position: 115
reference: P51016-1

22576483515053614tt119
type: Position
type: ExactPosition
position: 119
reference: P51016-1

22576483515053614tt122
type: Position
type: ExactPosition
position: 122
reference: P51016-1

22576483515053614tt123
type: Position
type: ExactPosition
position: 123
reference: P51016-1

22576483515053614tt132
type: Position
type: ExactPosition
position: 132
reference: P51016-1

22576483515053614tt135
type: Position
type: ExactPosition
position: 135
reference: P51016-1

22576483515053614tt142
type: Position
type: ExactPosition
position: 142
reference: P51016-1

22576483515053614tt148
type: Position
type: ExactPosition
position: 148
reference: P51016-1

22576483515053614tt161
type: Position
type: ExactPosition
position: 161
reference: P51016-1

22576483515053614tt164
type: Position
type: ExactPosition
position: 164
reference: P51016-1

22576483515053614tt169
type: Position
type: ExactPosition
position: 169
reference: P51016-1

22576483515053614tt177
type: Position
type: ExactPosition
position: 177
reference: P51016-1

22576483515053614tt190
type: Position
type: ExactPosition
position: 190
reference: P51016-1

22576483515053614tt195
type: Position
type: ExactPosition
position: 195
reference: P51016-1

22576483515053614tt208
type: Position
type: ExactPosition
position: 208
reference: P51016-1

22576483515053614tt217
type: Position
type: ExactPosition
position: 217
reference: P51016-1

22576483515053614tt222
type: Position
type: ExactPosition
position: 222
reference: P51016-1

22576483515053614tt226
type: Position
type: ExactPosition
position: 226
reference: P51016-1

22576483515053614tt227
type: Position
type: ExactPosition
position: 227
reference: P51016-1

22576483515053614tt229
type: Position
type: ExactPosition
position: 229
reference: P51016-1

22576483515053614tt239
type: Position
type: ExactPosition
position: 239
reference: P51016-1

22576483515053614tt249
type: Position
type: ExactPosition
position: 249
reference: P51016-1

22576483515053614tt257
type: Position
type: ExactPosition
position: 257
reference: P51016-1

22576483515053614tt266
type: Position
type: ExactPosition
position: 266
reference: P51016-1

22576483515053614tt269
type: Position
type: ExactPosition
position: 269
reference: P51016-1

22576483515053614tt271
type: Position
type: ExactPosition
position: 271
reference: P51016-1

22576483515053614tt280
type: Position
type: ExactPosition
position: 280
reference: P51016-1

22576483515053614tt288
type: Position
type: ExactPosition
position: 288
reference: P51016-1

22576483515053614tt294
type: Position
type: ExactPosition
position: 294
reference: P51016-1

22576483515053614tt305
type: Position
type: ExactPosition
position: 305
reference: P51016-1

22576483515053614tt309
type: Position
type: ExactPosition
position: 309
reference: P51016-1

22576483515053614tt319
type: Position
type: ExactPosition
position: 319
reference: P51016-1

22576483515053614tt327
type: Position
type: ExactPosition
position: 327
reference: P51016-1

22576483515053614tt340
type: Position
type: ExactPosition
position: 340
reference: P51016-1

substrate

P51016-1
type: Simple Sequence
modified: 1996-10-01
version: 1
mass: 37471
value: MTFNPSKKLYISDVTLRDGSHAIRHQYTLDDVRAIARALDKAKVDSIEVAHGDGLQGSSFNYGFGRHTDLEYIEAVAGEISHAQIATLLLPGIGSVHDLKNAYQAGARVVRVATHCTEADVSKQHIEYARNLGMDTVGFLMMSHMIPAEKLAEQGKLMESYGATCIYMADSGGAMSMNDIRDRMRAFKAVLKPETQVGMHAHHNLSLGVANSIVAVEEGCDRVDASLAGMGAGAGNAPLEVFIAVAERLGWNHGTDLYTLMDAADDIVRPLQDRPVRVDRETLGLGYAGVYSSFLRHAEIAAAKYNLKTLDILVELGHRRMVGGQEDMIVDVALDLLAAHKENRA

http://purl.uniprot.org/uniprot/P51016#attribution-44F726B6481B3054005E8DE690D0A2B6
creator: UniProtKB
manual: true
evidence: ECO_0000314
source: http://purl.uniprot.org/citations/8419288

http://purl.uniprot.org/uniprot/P51016#attribution-A474DB4F1AC35E93635077AA1A324865
creator: UniProtKB
manual: true
evidence: ECO_0000314
source: http://purl.uniprot.org/citations/12764229

http://purl.uniprot.org/uniprot/P51016#attribution-0FFC91602080964BBF1B0641543A1A0F
manual: true
evidence: ECO_0000255
source: MF_01656

http://purl.uniprot.org/uniprot/P51016#attribution-2896F89442AE0BAB4AA8721FBD8C1C43
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/12764229

http://purl.uniprot.org/uniprot/P51016#attribution-5BE3D9FC39CCB10D8A4B1CB4C5034062
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/1732207

http://purl.uniprot.org/uniprot/P51016#attribution-09B7B99FA8656CCA3E840206F13D3A3B
manual: true
evidence: ECO_0000269
source: http://purl.uniprot.org/citations/8419288

P80064
type: Protein
reviewed: true
created: 1992-05-01
modified: 2023-09-13
version: 112
mnemonic: HPPD_PSEUJ
old mnemonic: HPPD_PSESP
citation: http://purl.uniprot.org/citations/1572351
citation: http://purl.uniprot.org/citations/10467142
recommended name: SIP0F45D3AE9FACB9EE
enzyme: http://purl.uniprot.org/enzyme/1.13.11.27
organism: http://purl.uniprot.org/taxonomy/72587
annotation: SIP5E3EC37587EF2FED
annotation: SIP77D138CC4ADC4B86
annotation: SIP35AFEEE5C81349EB
annotation: SIPAAC28D8435016A3C
annotation: SIP3C911FEF13FB3AEB
annotation: PRO_0000088408
annotation: SIP6F59CBC165CCBE86
annotation: SIP422A4AC88A0C6E00
annotation: SIP63D249BE1010D67E
annotation: SIP3C379514B1F59082
annotation: SIP6959E9BB9A9AE9F9
annotation: SIP9950E3184C10979D
annotation: SIPA62C2253606C3AEE
annotation: SIPEABD76600DDEDE7B
annotation: SIP05144865B3AF793D
annotation: SIP14A28FAA94C8D1EA
annotation: SIP4018A70B5E50B2C8
annotation: SIPE67E961F71481FC8
annotation: SIPFAA33B7032B57AD8
annotation: SIPED91C525D973FE78
annotation: SIP62ABA5255FF22CD1
annotation: SIP3898BFAD482F9FEF
annotation: SIP938C0CBFF9DBC7EE
annotation: SIPEAC7641D745E14B9
annotation: SIP334B6860A02D3F38
annotation: SIP2869AC00302D3884
annotation: SIP0B1B643F9972AC46
annotation: SIP692346BDDFED8F8C
annotation: SIPE287ECCFDE50860A
annotation: SIP3416631A34C849CE
annotation: SIPEF0816377638DB62
annotation: SIP5A0F47C9B8F2F058
annotation: SIPCA2A2617A9023939
annotation: SIPBC5396623A75027F
annotation: SIPEB5A125547ED9F77
annotation: SIPC6E304389DF2CB04
annotation: SIP620D120729D27BA8
annotation: SIP45F9ABA5E9AE795D
annotation: SIP699F422456BCB6A9
annotation: SIPF789C3A818964235
annotation: SIP9A8925D47BC25D36
existence: Evidence at Protein Level Existence
classified with: http://purl.uniprot.org/keywords/2
classified with: http://purl.uniprot.org/keywords/223
classified with: http://purl.uniprot.org/keywords/903
classified with: http://purl.uniprot.org/keywords/408
classified with: http://purl.uniprot.org/keywords/479
classified with: http://purl.uniprot.org/keywords/585
classified with: http://purl.uniprot.org/keywords/677
classified with: http://purl.uniprot.org/keywords/828
classified with: GO_0003868
classified with: GO_0046872
classified with: GO_0006559
classified with: GO_0006572
sequence: P80064-1

P80064-citation-1572351
type: Citation Statement
scope: PROTEIN SEQUENCE

P80064-citation-10467142
type: Citation Statement
scope: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS)

1CJX
type: Structure Resource
database: PDB
method: X-Ray Crystallography
resolution: 2.40
chain sequence mapping: PDB 1CJX tt1tt357

PDB 1CJX tt1tt357
chain: A/B/C/D=1-357

http://purl.uniprot.org/brenda/1.13.11.27
type: Resource
database: BRENDA
comment: 5121

cd07250
type: Resource
database: CDD
comment: HPPD_C_like
signature sequence match: CDD cd07250 match 1

cd08342
type: Resource
database: CDD
comment: HPPD_N_like
signature sequence match: CDD cd08342 match 1

IPR005956
type: Resource
database: InterPro
comment: 4OHPhenylPyrv_dOase

IPR041735
type: Resource
database: InterPro
comment: 4OHPhenylPyrv_dOase_C

IPR041736
type: Resource
database: InterPro
comment: 4OHPhenylPyrv_dOase_N

TIGR01263
type: Resource
database: NCBIfam
comment: 4HPPD
signature sequence match: NCBIfam TIGR01263 match 1

PTHR11959
type: Resource
database: PANTHER
comment: 4-HYDROXYPHENYLPYRUVATE DIOXYGENASE
signature sequence match: PANTHER PTHR11959 match 1

PTHR11959:SF1
type: Resource
database: PANTHER
comment: 4-HYDROXYPHENYLPYRUVATE DIOXYGENASE
signature sequence match: PANTHER PTHR11959:SF1 match 1

PF14696
type: Resource
database: Pfam
comment: Glyoxalase_5
signature sequence match: Pfam PF14696 match 1

PIRSF009283
type: Resource
database: PIRSF
comment: HPP_dOase
signature sequence match: PIRSF PIRSF009283 match 1

SIP0F45D3AE9FACB9EE
type: Structured Name
full name: 4-hydroxyphenylpyruvate dioxygenase
short name: 4HPPD
short name: HPD
short name: HPPDase
ec name: 1.13.11.27

hpd
type: Gene

SIP77D138CC4ADC4B86
type: Cofactor Annotation
comment: Binds 1 Fe cation per subunit.
cofactor: CHEBI_24875

SIPAAC28D8435016A3C
type: Subunit Annotation
comment: Homotetramer.

SIP3C911FEF13FB3AEB
type: Similarity Annotation
comment: Belongs to the 4HPPD family.

PRO_0000088408
type: Chain Annotation
comment: 4-hydroxyphenylpyruvate dioxygenase
mass: 40061
range: 22579777755296814tt1tt357

22579777755296814tt1
type: Position
type: ExactPosition
position: 1
reference: P80064-1

22579777755296814tt357
type: Position
type: ExactPosition
position: 357
reference: P80064-1

SIP6F59CBC165CCBE86
type: Domain Extent Annotation
comment: VOC 1
range: 22579777755296814tt12tt129

22579777755296814tt12
type: Position
type: ExactPosition
position: 12
reference: P80064-1

22579777755296814tt129
type: Position
type: ExactPosition
position: 129
reference: P80064-1

SIP422A4AC88A0C6E00
type: Domain Extent Annotation
comment: VOC 2
range: 22579777755296814tt158tt313

22579777755296814tt158
type: Position
type: ExactPosition
position: 158
reference: P80064-1

22579777755296814tt313
type: Position
type: ExactPosition
position: 313
reference: P80064-1

22579777755296814tt161
type: Position
type: ExactPosition
position: 161
reference: P80064-1

22579777755296814tt240
type: Position
type: ExactPosition
position: 240
reference: P80064-1

22579777755296814tt322
type: Position
type: ExactPosition
position: 322
reference: P80064-1

22579777755296814tt10
type: Position
type: ExactPosition
position: 10
reference: P80064-1

22579777755296814tt19
type: Position
type: ExactPosition
position: 19
reference: P80064-1

22579777755296814tt27
type: Position
type: ExactPosition
position: 27
reference: P80064-1

22579777755296814tt32
type: Position
type: ExactPosition
position: 32
reference: P80064-1

22579777755296814tt36
type: Position
type: ExactPosition
position: 36
reference: P80064-1

22579777755296814tt51
type: Position
type: ExactPosition
position: 51
reference: P80064-1

22579777755296814tt54
type: Position
type: ExactPosition
position: 54
reference: P80064-1

22579777755296814tt59
type: Position
type: ExactPosition
position: 59
reference: P80064-1

22579777755296814tt62
type: Position
type: ExactPosition
position: 62
reference: P80064-1

22579777755296814tt64
type: Position
type: ExactPosition
position: 64
reference: P80064-1

22579777755296814tt65
type: Position
type: ExactPosition
position: 65
reference: P80064-1

22579777755296814tt73
type: Position
type: ExactPosition
position: 73
reference: P80064-1